Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08403.t1 | KEH31436.1 | 96.8 | 187 | 5 | 1 | 1 | 186 | 34 | 220 | 2.60E-97 | 364.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08403.t1 | Q9SCY2 | 59.2 | 196 | 62 | 5 | 1 | 186 | 1 | 188 | 9.4e-56 | 218.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08403.t1 | A0A072UQ15 | 96.8 | 187 | 5 | 1 | 1 | 186 | 34 | 220 | 1.9e-97 | 364.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene08403 | 0.82324 | 1.54E-53 | -1.69E-46 |
| MS.gene049171 | MS.gene08403 | 0.827679 | 1.37E-54 | -1.69E-46 |
| MS.gene049217 | MS.gene08403 | 0.814788 | 1.29E-51 | -1.69E-46 |
| MS.gene049222 | MS.gene08403 | 0.843132 | 1.69E-58 | -1.69E-46 |
| MS.gene049285 | MS.gene08403 | 0.867193 | 1.64E-65 | -1.69E-46 |
| MS.gene049320 | MS.gene08403 | 0.850933 | 1.23E-60 | -1.69E-46 |
| MS.gene049609 | MS.gene08403 | 0.852852 | 3.52E-61 | -1.69E-46 |
| MS.gene049805 | MS.gene08403 | 0.855691 | 5.32E-62 | -1.69E-46 |
| MS.gene05010 | MS.gene08403 | 0.836651 | 8.26E-57 | -1.69E-46 |
| MS.gene050163 | MS.gene08403 | 0.825195 | 5.35E-54 | -1.69E-46 |
| MS.gene050333 | MS.gene08403 | 0.829351 | 5.40E-55 | -1.69E-46 |
| MS.gene050504 | MS.gene08403 | 0.827072 | 1.91E-54 | -1.69E-46 |
| MS.gene050518 | MS.gene08403 | 0.841518 | 4.53E-58 | -1.69E-46 |
| MS.gene050520 | MS.gene08403 | 0.813043 | 3.13E-51 | -1.69E-46 |
| MS.gene050538 | MS.gene08403 | 0.823777 | 1.15E-53 | -1.69E-46 |
| MS.gene050577 | MS.gene08403 | 0.805105 | 1.57E-49 | -1.69E-46 |
| MS.gene050593 | MS.gene08403 | 0.844388 | 7.80E-59 | -1.69E-46 |
| MS.gene050612 | MS.gene08403 | 0.823316 | 1.48E-53 | -1.69E-46 |
| MS.gene050699 | MS.gene08403 | 0.832949 | 7.06E-56 | -1.69E-46 |
| MS.gene050747 | MS.gene08403 | 0.865673 | 4.98E-65 | -1.69E-46 |
| MS.gene050772 | MS.gene08403 | 0.881357 | 2.57E-70 | -1.69E-46 |
| MS.gene05080 | MS.gene08403 | 0.821035 | 5.00E-53 | -1.69E-46 |
| MS.gene050950 | MS.gene08403 | 0.855439 | 6.30E-62 | -1.69E-46 |
| MS.gene050986 | MS.gene08403 | 0.813744 | 2.19E-51 | -1.69E-46 |
| MS.gene05100 | MS.gene08403 | 0.833379 | 5.52E-56 | -1.69E-46 |
| MS.gene05105 | MS.gene08403 | 0.837216 | 5.93E-57 | -1.69E-46 |
| MS.gene051387 | MS.gene08403 | 0.856378 | 3.35E-62 | -1.69E-46 |
| MS.gene051551 | MS.gene08403 | 0.811524 | 6.71E-51 | -1.69E-46 |
| MS.gene051666 | MS.gene08403 | 0.820346 | 7.20E-53 | -1.69E-46 |
| MS.gene051694 | MS.gene08403 | 0.801177 | 1.02E-48 | -1.69E-46 |
| MS.gene052271 | MS.gene08403 | 0.831437 | 1.67E-55 | -1.69E-46 |
| MS.gene052379 | MS.gene08403 | 0.818149 | 2.28E-52 | -1.69E-46 |
| MS.gene052421 | MS.gene08403 | 0.85092 | 1.24E-60 | -1.69E-46 |
| MS.gene052482 | MS.gene08403 | 0.804788 | 1.83E-49 | -1.69E-46 |
| MS.gene052486 | MS.gene08403 | 0.808156 | 3.56E-50 | -1.69E-46 |
| MS.gene052501 | MS.gene08403 | 0.808108 | 3.64E-50 | -1.69E-46 |
| MS.gene052503 | MS.gene08403 | 0.822826 | 1.92E-53 | -1.69E-46 |
| MS.gene052505 | MS.gene08403 | 0.807591 | 4.69E-50 | -1.69E-46 |
| MS.gene052615 | MS.gene08403 | 0.85372 | 1.98E-61 | -1.69E-46 |
| MS.gene052823 | MS.gene08403 | 0.812347 | 4.44E-51 | -1.69E-46 |
| MS.gene052825 | MS.gene08403 | 0.804581 | 2.02E-49 | -1.69E-46 |
| MS.gene052916 | MS.gene08403 | 0.800159 | 1.64E-48 | -1.69E-46 |
| MS.gene052982 | MS.gene08403 | 0.801261 | 9.79E-49 | -1.69E-46 |
| MS.gene053103 | MS.gene08403 | 0.818745 | 1.67E-52 | -1.69E-46 |
| MS.gene053104 | MS.gene08403 | 0.831245 | 1.86E-55 | -1.69E-46 |
| MS.gene053107 | MS.gene08403 | 0.805715 | 1.17E-49 | -1.69E-46 |
| MS.gene053207 | MS.gene08403 | 0.821637 | 3.63E-53 | -1.69E-46 |
| MS.gene053213 | MS.gene08403 | 0.882559 | 9.41E-71 | -1.69E-46 |
| MS.gene053286 | MS.gene08403 | 0.877841 | 4.55E-69 | -1.69E-46 |
| MS.gene053322 | MS.gene08403 | 0.835141 | 1.99E-56 | -1.69E-46 |
| MS.gene053436 | MS.gene08403 | 0.863714 | 2.04E-64 | -1.69E-46 |
| MS.gene053479 | MS.gene08403 | 0.806353 | 8.57E-50 | -1.69E-46 |
| MS.gene053480 | MS.gene08403 | 0.801407 | 9.14E-49 | -1.69E-46 |
| MS.gene053574 | MS.gene08403 | 0.861977 | 7.02E-64 | -1.69E-46 |
| MS.gene053579 | MS.gene08403 | 0.821805 | 3.32E-53 | -1.69E-46 |
| MS.gene053626 | MS.gene08403 | 0.837437 | 5.20E-57 | -1.69E-46 |
| MS.gene053632 | MS.gene08403 | 0.829651 | 4.57E-55 | -1.69E-46 |
| MS.gene053824 | MS.gene08403 | 0.826779 | 2.25E-54 | -1.69E-46 |
| MS.gene053916 | MS.gene08403 | 0.838559 | 2.68E-57 | -1.69E-46 |
| MS.gene054408 | MS.gene08403 | 0.86156 | 9.41E-64 | -1.69E-46 |
| MS.gene054595 | MS.gene08403 | 0.85057 | 1.56E-60 | -1.69E-46 |
| MS.gene054600 | MS.gene08403 | 0.832905 | 7.24E-56 | -1.69E-46 |
| MS.gene054642 | MS.gene08403 | 0.854484 | 1.19E-61 | -1.69E-46 |
| MS.gene054927 | MS.gene08403 | 0.809859 | 1.54E-50 | -1.69E-46 |
| MS.gene054954 | MS.gene08403 | 0.828992 | 6.60E-55 | -1.69E-46 |
| MS.gene05505 | MS.gene08403 | 0.865254 | 6.75E-65 | -1.69E-46 |
| MS.gene055206 | MS.gene08403 | 0.809775 | 1.60E-50 | -1.69E-46 |
| MS.gene055212 | MS.gene08403 | 0.814569 | 1.44E-51 | -1.69E-46 |
| MS.gene055224 | MS.gene08403 | 0.840565 | 8.05E-58 | -1.69E-46 |
| MS.gene055289 | MS.gene08403 | 0.831338 | 1.77E-55 | -1.69E-46 |
| MS.gene055362 | MS.gene08403 | 0.841354 | 5.00E-58 | -1.69E-46 |
| MS.gene055412 | MS.gene08403 | 0.817602 | 3.03E-52 | -1.69E-46 |
| MS.gene055502 | MS.gene08403 | 0.855435 | 6.32E-62 | -1.69E-46 |
| MS.gene055638 | MS.gene08403 | 0.800862 | 1.18E-48 | -1.69E-46 |
| MS.gene055647 | MS.gene08403 | 0.805647 | 1.21E-49 | -1.69E-46 |
| MS.gene055722 | MS.gene08403 | 0.828677 | 7.87E-55 | -1.69E-46 |
| MS.gene055748 | MS.gene08403 | 0.80121 | 1.00E-48 | -1.69E-46 |
| MS.gene055750 | MS.gene08403 | 0.815933 | 7.17E-52 | -1.69E-46 |
| MS.gene05592 | MS.gene08403 | 0.849071 | 4.09E-60 | -1.69E-46 |
| MS.gene055941 | MS.gene08403 | 0.829489 | 5.00E-55 | -1.69E-46 |
| MS.gene056040 | MS.gene08403 | 0.819816 | 9.52E-53 | -1.69E-46 |
| MS.gene056374 | MS.gene08403 | 0.814459 | 1.52E-51 | -1.69E-46 |
| MS.gene056422 | MS.gene08403 | 0.821193 | 4.60E-53 | -1.69E-46 |
| MS.gene056446 | MS.gene08403 | 0.842367 | 2.70E-58 | -1.69E-46 |
| MS.gene056504 | MS.gene08403 | 0.812465 | 4.18E-51 | -1.69E-46 |
| MS.gene056510 | MS.gene08403 | 0.819009 | 1.45E-52 | -1.69E-46 |
| MS.gene056657 | MS.gene08403 | 0.86307 | 3.24E-64 | -1.69E-46 |
| MS.gene056692 | MS.gene08403 | 0.809303 | 2.02E-50 | -1.69E-46 |
| MS.gene057011 | MS.gene08403 | 0.847934 | 8.45E-60 | -1.69E-46 |
| MS.gene057076 | MS.gene08403 | 0.837046 | 6.55E-57 | -1.69E-46 |
| MS.gene057129 | MS.gene08403 | 0.83617 | 1.09E-56 | -1.69E-46 |
| MS.gene057146 | MS.gene08403 | 0.815166 | 1.06E-51 | -1.69E-46 |
| MS.gene057331 | MS.gene08403 | 0.827685 | 1.36E-54 | -1.69E-46 |
| MS.gene057416 | MS.gene08403 | 0.875075 | 4.10E-68 | -1.69E-46 |
| MS.gene057831 | MS.gene08403 | 0.835005 | 2.16E-56 | -1.69E-46 |
| MS.gene05787 | MS.gene08403 | 0.830687 | 2.55E-55 | -1.69E-46 |
| MS.gene058019 | MS.gene08403 | 0.8471 | 1.43E-59 | -1.69E-46 |
| MS.gene058130 | MS.gene08403 | 0.845392 | 4.18E-59 | -1.69E-46 |
| MS.gene058293 | MS.gene08403 | 0.814701 | 1.35E-51 | -1.69E-46 |
| MS.gene058323 | MS.gene08403 | 0.825727 | 4.00E-54 | -1.69E-46 |
| MS.gene058338 | MS.gene08403 | 0.809233 | 2.09E-50 | -1.69E-46 |
| MS.gene05837 | MS.gene08403 | 0.872508 | 3.01E-67 | -1.69E-46 |
| MS.gene058419 | MS.gene08403 | 0.872011 | 4.41E-67 | -1.69E-46 |
| MS.gene058544 | MS.gene08403 | 0.840255 | 9.71E-58 | -1.69E-46 |
| MS.gene058773 | MS.gene08403 | 0.829117 | 6.16E-55 | -1.69E-46 |
| MS.gene058838 | MS.gene08403 | 0.806546 | 7.80E-50 | -1.69E-46 |
| MS.gene059022 | MS.gene08403 | 0.820099 | 8.20E-53 | -1.69E-46 |
| MS.gene059023 | MS.gene08403 | 0.833189 | 6.15E-56 | -1.69E-46 |
| MS.gene059029 | MS.gene08403 | 0.817504 | 3.19E-52 | -1.69E-46 |
| MS.gene05934 | MS.gene08403 | 0.820999 | 5.10E-53 | -1.69E-46 |
| MS.gene059367 | MS.gene08403 | 0.802893 | 4.52E-49 | -1.69E-46 |
| MS.gene059368 | MS.gene08403 | 0.802526 | 5.38E-49 | -1.69E-46 |
| MS.gene059536 | MS.gene08403 | 0.808188 | 3.50E-50 | -1.69E-46 |
| MS.gene059667 | MS.gene08403 | 0.80474 | 1.87E-49 | -1.69E-46 |
| MS.gene059677 | MS.gene08403 | 0.842422 | 2.61E-58 | -1.69E-46 |
| MS.gene059718 | MS.gene08403 | 0.841411 | 4.83E-58 | -1.69E-46 |
| MS.gene059756 | MS.gene08403 | 0.816 | 6.93E-52 | -1.69E-46 |
| MS.gene059801 | MS.gene08403 | 0.810153 | 1.33E-50 | -1.69E-46 |
| MS.gene059960 | MS.gene08403 | 0.840657 | 7.62E-58 | -1.69E-46 |
| MS.gene060193 | MS.gene08403 | 0.800103 | 1.69E-48 | -1.69E-46 |
| MS.gene06077 | MS.gene08403 | 0.833466 | 5.25E-56 | -1.69E-46 |
| MS.gene060804 | MS.gene08403 | 0.816491 | 5.38E-52 | -1.69E-46 |
| MS.gene060835 | MS.gene08403 | 0.83643 | 9.40E-57 | -1.69E-46 |
| MS.gene060837 | MS.gene08403 | 0.805364 | 1.38E-49 | -1.69E-46 |
| MS.gene06092 | MS.gene08403 | 0.875497 | 2.94E-68 | -1.69E-46 |
| MS.gene061150 | MS.gene08403 | 0.845365 | 4.25E-59 | -1.69E-46 |
| MS.gene061171 | MS.gene08403 | 0.801977 | 6.98E-49 | -1.69E-46 |
| MS.gene061200 | MS.gene08403 | 0.845547 | 3.79E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08403.t1 | MTR_4g094785 | 96.791 | 187 | 5 | 1 | 1 | 186 | 34 | 220 | 1.25e-130 | 367 |
| MS.gene08403.t1 | MTR_8g091410 | 54.386 | 114 | 51 | 1 | 74 | 187 | 76 | 188 | 3.69e-36 | 125 |
| MS.gene08403.t1 | MTR_8g012380 | 50.633 | 79 | 33 | 1 | 103 | 181 | 46 | 118 | 2.69e-20 | 83.2 |
| MS.gene08403.t1 | MTR_5g008680 | 40.909 | 110 | 55 | 4 | 77 | 183 | 381 | 483 | 1.86e-16 | 77.0 |
| MS.gene08403.t1 | MTR_4g068190 | 41.414 | 99 | 50 | 2 | 78 | 176 | 103 | 193 | 9.69e-14 | 67.8 |
| MS.gene08403.t1 | MTR_8g080980 | 41.333 | 75 | 38 | 1 | 107 | 181 | 56 | 124 | 6.36e-12 | 63.9 |
| MS.gene08403.t1 | MTR_7g053470 | 40.000 | 75 | 39 | 1 | 107 | 181 | 52 | 120 | 1.04e-11 | 63.2 |
| MS.gene08403.t1 | MTR_8g012340 | 41.333 | 75 | 38 | 1 | 107 | 181 | 58 | 126 | 2.01e-11 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08403.t1 | AT5G45680 | 59.184 | 196 | 62 | 5 | 1 | 186 | 1 | 188 | 1.83e-72 | 218 |
| MS.gene08403.t1 | AT4G39710 | 53.774 | 106 | 49 | 0 | 82 | 187 | 87 | 192 | 1.83e-36 | 127 |
| MS.gene08403.t1 | AT4G39710 | 53.774 | 106 | 49 | 0 | 82 | 187 | 87 | 192 | 1.83e-36 | 127 |
| MS.gene08403.t1 | AT4G39710 | 55.696 | 79 | 35 | 0 | 82 | 160 | 87 | 165 | 1.60e-27 | 102 |
| MS.gene08403.t1 | AT5G48580 | 51.899 | 79 | 32 | 1 | 103 | 181 | 48 | 120 | 1.24e-20 | 84.7 |
| MS.gene08403.t1 | AT3G25220 | 50.633 | 79 | 33 | 1 | 103 | 181 | 48 | 120 | 6.72e-20 | 82.4 |
| MS.gene08403.t1 | AT4G25340 | 39.130 | 115 | 60 | 4 | 71 | 182 | 351 | 458 | 1.21e-17 | 80.5 |
| MS.gene08403.t1 | AT5G05420 | 47.619 | 84 | 36 | 3 | 101 | 183 | 50 | 126 | 5.76e-17 | 74.3 |
| MS.gene08403.t1 | AT2G43560 | 45.745 | 94 | 43 | 2 | 78 | 171 | 41 | 126 | 3.20e-15 | 70.5 |
| MS.gene08403.t1 | AT2G43560 | 45.745 | 94 | 43 | 2 | 78 | 171 | 97 | 182 | 3.36e-15 | 71.6 |
| MS.gene08403.t1 | AT5G48570 | 34.711 | 121 | 59 | 5 | 71 | 181 | 23 | 133 | 1.50e-12 | 65.9 |
| MS.gene08403.t1 | AT3G60370 | 38.202 | 89 | 49 | 1 | 87 | 175 | 118 | 200 | 1.33e-11 | 62.0 |
| MS.gene08403.t1 | AT3G60370 | 38.202 | 89 | 49 | 1 | 87 | 175 | 147 | 229 | 1.72e-11 | 62.0 |
| MS.gene08403.t1 | AT3G25230 | 40.000 | 75 | 39 | 1 | 107 | 181 | 57 | 125 | 1.80e-11 | 62.8 |
| MS.gene08403.t1 | AT3G25230 | 40.000 | 75 | 39 | 1 | 107 | 181 | 57 | 125 | 2.09e-11 | 62.4 |
| MS.gene08403.t1 | AT3G10060 | 38.679 | 106 | 50 | 4 | 83 | 180 | 99 | 197 | 2.68e-11 | 60.8 |
Find 51 sgRNAs with CRISPR-Local
Find 89 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGAAGCTTCCTCCAGAATT+TGG | 0.175545 | 4.1:-17605282 | MS.gene08403:CDS |
| TATAATGATAGCAAATTAAA+AGG | 0.230024 | 4.1:+17605197 | None:intergenic |
| ATAGGCCTCTCCTTGAGTTT+TGG | 0.272868 | 4.1:-17606286 | MS.gene08403:CDS |
| TCTTATTGTGATAAAATTGT+AGG | 0.291291 | 4.1:-17606172 | MS.gene08403:CDS |
| CTATGGTCCTCAGGCTGTTA+AGG | 0.309962 | 4.1:-17606149 | MS.gene08403:CDS |
| CTTGAGCCATATCCAAATTC+TGG | 0.313714 | 4.1:+17605270 | None:intergenic |
| TGTTAAGGGACAACTTATTA+AGG | 0.315282 | 4.1:-17606134 | MS.gene08403:intron |
| GGAATTCCTCCTATGCTTAC+AGG | 0.339705 | 4.1:-17605402 | MS.gene08403:intron |
| CTTCCTCCAGAATTTGGATA+TGG | 0.340666 | 4.1:-17605276 | MS.gene08403:CDS |
| GCTGTTGCAGTAGGTATTAT+TGG | 0.348854 | 4.1:+17606241 | None:intergenic |
| AGGCAAGTCCCAACTGAATA+TGG | 0.354350 | 4.1:+17606430 | None:intergenic |
| AGAACATGTTGTTAGTGAAT+TGG | 0.362479 | 4.1:+17606375 | None:intergenic |
| GGCACATTATGTGGGGAGAT+TGG | 0.379326 | 4.1:-17605864 | MS.gene08403:CDS |
| GAATTAACTGTAGCTCCTTC+AGG | 0.411871 | 4.1:-17606199 | MS.gene08403:CDS |
| CTGGTGGTTGTTGTTGGACT+TGG | 0.423685 | 4.1:+17606350 | None:intergenic |
| TTAATGTTAGGCACATTATG+TGG | 0.439371 | 4.1:-17605873 | MS.gene08403:intron |
| ACCACCAGTTCTTGTACAAA+AGG | 0.466640 | 4.1:-17606335 | MS.gene08403:CDS |
| TGTGATAAAATTGTAGGCTA+TGG | 0.467135 | 4.1:-17606166 | MS.gene08403:CDS |
| CAAGAACTGGTGGTTGTTGT+TGG | 0.468643 | 4.1:+17606344 | None:intergenic |
| CTGAAAAGAAGAGAAGCAAT+AGG | 0.473925 | 4.1:-17606304 | MS.gene08403:CDS |
| TATGGTCCTCAGGCTGTTAA+GGG | 0.475010 | 4.1:-17606148 | MS.gene08403:CDS |
| CACTAGCACCATATTCAGTT+GGG | 0.486666 | 4.1:-17606438 | MS.gene08403:CDS |
| AATTGTAGGCTATGGTCCTC+AGG | 0.493523 | 4.1:-17606158 | MS.gene08403:CDS |
| CTTACATTTCGTGTTGGTGT+TGG | 0.498679 | 4.1:-17605797 | MS.gene08403:CDS |
| TCAAGAGGAGCTGGTTGTAA+AGG | 0.503208 | 4.1:-17605252 | MS.gene08403:CDS |
| TCACTAGCACCATATTCAGT+TGG | 0.504892 | 4.1:-17606439 | MS.gene08403:CDS |
| GGATATGGCTCAAGAGGAGC+TGG | 0.513862 | 4.1:-17605261 | MS.gene08403:CDS |
| TCATATAGGTTATCAAAGGA+TGG | 0.519848 | 4.1:-17605449 | MS.gene08403:intron |
| GTTATCAAAGGATGGGATGT+AGG | 0.520177 | 4.1:-17605441 | MS.gene08403:CDS |
| CAGGTACCTGTAAGCATAGG+AGG | 0.528701 | 4.1:+17605396 | None:intergenic |
| TAATGTTAGGCACATTATGT+GGG | 0.541025 | 4.1:-17605872 | MS.gene08403:intron |
| ACACCAACACGAAATGTAAG+TGG | 0.551590 | 4.1:+17605800 | None:intergenic |
| GCTTCAGCTGCTGTTGCAGT+AGG | 0.562444 | 4.1:+17606232 | None:intergenic |
| GAATTTGGATATGGCTCAAG+AGG | 0.566038 | 4.1:-17605267 | MS.gene08403:CDS |
| ATTTCGTGTTGGTGTTGGAG+AGG | 0.570960 | 4.1:-17605792 | MS.gene08403:intron |
| TTTGGGGCTGCAAGGAGGTG+AGG | 0.573241 | 4.1:+17606410 | None:intergenic |
| AAGCCACTTACATTTCGTGT+TGG | 0.577359 | 4.1:-17605803 | MS.gene08403:CDS |
| CATATAGGTTATCAAAGGAT+GGG | 0.577493 | 4.1:-17605448 | MS.gene08403:intron |
| TATGTGGGGAGATTGGAGAA+TGG | 0.578143 | 4.1:-17605857 | MS.gene08403:CDS |
| ATGTTGTTAGTGAATTGGAA+TGG | 0.592486 | 4.1:+17606380 | None:intergenic |
| TCACAATAAGAAAGGCCTGA+AGG | 0.595988 | 4.1:+17606184 | None:intergenic |
| TGAATCATATAGGTTATCAA+AGG | 0.598952 | 4.1:-17605453 | MS.gene08403:intron |
| AAACAGGTACCTGTAAGCAT+AGG | 0.604775 | 4.1:+17605393 | None:intergenic |
| AGAGGAGCTGGTTGTAAAGG+AGG | 0.607049 | 4.1:-17605249 | MS.gene08403:CDS |
| ATGTAGAAGACCAAAACTCA+AGG | 0.618115 | 4.1:+17606276 | None:intergenic |
| TTCGACAGCAGCTACAATCG+TGG | 0.627562 | 4.1:-17605827 | MS.gene08403:CDS |
| AATGTTAGGCACATTATGTG+GGG | 0.643650 | 4.1:-17605871 | MS.gene08403:intron |
| GGAGCTACAGTTAATTCACA+TGG | 0.644123 | 4.1:+17606205 | None:intergenic |
| GAGCCATATCCAAATTCTGG+AGG | 0.647151 | 4.1:+17605273 | None:intergenic |
| GAAGACCAAAACTCAAGGAG+AGG | 0.650327 | 4.1:+17606281 | None:intergenic |
| AGTTGTCCCTTAACAGCCTG+AGG | 0.654589 | 4.1:+17606142 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATATATGATAAAAGTAAAA+TGG | - | chr4.1:17605601-17605620 | MS.gene08403:intron | 10.0% |
| !! | ATATTATTCATGAATCATAT+AGG | - | chr4.1:17606194-17606213 | MS.gene08403:CDS | 15.0% |
| !!! | GTAAATTCTATTTTGTTTTA+TGG | - | chr4.1:17606298-17606317 | MS.gene08403:CDS | 15.0% |
| !! | GTTAATAAACCAAAAAGTAA+AGG | - | chr4.1:17606074-17606093 | MS.gene08403:intron | 20.0% |
| !! | TATAATGGTCAAATTAAACA+CGG | + | chr4.1:17605764-17605783 | None:intergenic | 20.0% |
| !! | TCTTATTGTGATAAAATTGT+AGG | - | chr4.1:17605485-17605504 | MS.gene08403:intron | 20.0% |
| !! | TTGACCATTATATTAATGTT+AGG | - | chr4.1:17605772-17605791 | MS.gene08403:intron | 20.0% |
| !!! | ATTTTTTATTCTATGTTAGG+AGG | - | chr4.1:17606342-17606361 | MS.gene08403:CDS | 20.0% |
| !!! | CAATTTTATCACAATAAGAA+AGG | + | chr4.1:17605484-17605503 | None:intergenic | 20.0% |
| !!! | GACATTTTTTATTCTATGTT+AGG | - | chr4.1:17606339-17606358 | MS.gene08403:CDS | 20.0% |
| ! | ACTAACTAACTTCATTTCAT+CGG | + | chr4.1:17605665-17605684 | None:intergenic | 25.0% |
| ! | ATAATGGTCAAATTAAACAC+GGG | + | chr4.1:17605763-17605782 | None:intergenic | 25.0% |
| ! | TGAATCATATAGGTTATCAA+AGG | - | chr4.1:17606204-17606223 | MS.gene08403:CDS | 25.0% |
| ! | TGTAACAAGTAACAACTTAT+AGG | + | chr4.1:17606146-17606165 | None:intergenic | 25.0% |
| ! | TGTGCCTAACATTAATATAA+TGG | + | chr4.1:17605779-17605798 | None:intergenic | 25.0% |
| ! | TTCATGAATAATATTGAGCA+TGG | + | chr4.1:17606188-17606207 | None:intergenic | 25.0% |
| !! | AATTGGAATGGTTAAGTTTT+TGG | + | chr4.1:17605268-17605287 | None:intergenic | 25.0% |
| !! | ATTGGAATGGTTAAGTTTTT+GGG | + | chr4.1:17605267-17605286 | None:intergenic | 25.0% |
| !! | CCTTAAAATCTTTTATATCG+CGG | - | chr4.1:17606004-17606023 | MS.gene08403:intron | 25.0% |
| !! | TATAACTGACTTTGTATAGA+AGG | - | chr4.1:17605576-17605595 | MS.gene08403:intron | 25.0% |
| AGAACATGTTGTTAGTGAAT+TGG | + | chr4.1:17605285-17605304 | None:intergenic | 30.0% | |
| AGGTATCATAATTCTATCCA+TGG | - | chr4.1:17605885-17605904 | MS.gene08403:intron | 30.0% | |
| ATGTTGTTAGTGAATTGGAA+TGG | + | chr4.1:17605280-17605299 | None:intergenic | 30.0% | |
| CATATAGGTTATCAAAGGAT+GGG | - | chr4.1:17606209-17606228 | MS.gene08403:CDS | 30.0% | |
| CTGTTAACTGCAGTAAAAAA+TGG | + | chr4.1:17606046-17606065 | None:intergenic | 30.0% | |
| TAATGTTAGGCACATTATGT+GGG | - | chr4.1:17605785-17605804 | MS.gene08403:intron | 30.0% | |
| TCATATAGGTTATCAAAGGA+TGG | - | chr4.1:17606208-17606227 | MS.gene08403:CDS | 30.0% | |
| TGTGATAAAATTGTAGGCTA+TGG | - | chr4.1:17605491-17605510 | MS.gene08403:intron | 30.0% | |
| TGTTAAGGGACAACTTATTA+AGG | - | chr4.1:17605523-17605542 | MS.gene08403:intron | 30.0% | |
| TTAATGTTAGGCACATTATG+TGG | - | chr4.1:17605784-17605803 | MS.gene08403:intron | 30.0% | |
| TTTACAGTGACTGAAAAAAC+AGG | + | chr4.1:17606283-17606302 | None:intergenic | 30.0% | |
| ! | CCGCGATATAAAAGATTTTA+AGG | + | chr4.1:17606007-17606026 | None:intergenic | 30.0% |
| ! | GTAACGACAATTTAAAACCA+TGG | + | chr4.1:17605905-17605924 | None:intergenic | 30.0% |
| ! | TTGGAATGGTTAAGTTTTTG+GGG | + | chr4.1:17605266-17605285 | None:intergenic | 30.0% |
| !! | CTCATAAGACCTTTACTTTT+TGG | + | chr4.1:17606086-17606105 | None:intergenic | 30.0% |
| AATGTTAGGCACATTATGTG+GGG | - | chr4.1:17605786-17605805 | MS.gene08403:intron | 35.0% | |
| ATGTAGAAGACCAAAACTCA+AGG | + | chr4.1:17605384-17605403 | None:intergenic | 35.0% | |
| CAAATTAAACACGGGACAAA+GGG | + | chr4.1:17605755-17605774 | None:intergenic | 35.0% | |
| CTGAAAAGAAGAGAAGCAAT+AGG | - | chr4.1:17605353-17605372 | MS.gene08403:intron | 35.0% | |
| TCAAATTAAACACGGGACAA+AGG | + | chr4.1:17605756-17605775 | None:intergenic | 35.0% | |
| ! | AGGTCTTATGAGATGTTGAT+TGG | - | chr4.1:17606094-17606113 | MS.gene08403:intron | 35.0% |
| ! | TTCTCCTTTTGTACAAGAAC+TGG | + | chr4.1:17605329-17605348 | None:intergenic | 35.0% |
| AAACAGGTACCTGTAAGCAT+AGG | + | chr4.1:17606267-17606286 | None:intergenic | 40.0% | |
| AAGCCACTTACATTTCGTGT+TGG | - | chr4.1:17605854-17605873 | MS.gene08403:CDS | 40.0% | |
| ACACCAACACGAAATGTAAG+TGG | + | chr4.1:17605860-17605879 | None:intergenic | 40.0% | |
| ACCACCAGTTCTTGTACAAA+AGG | - | chr4.1:17605322-17605341 | MS.gene08403:intron | 40.0% | |
| CACTAGCACCATATTCAGTT+GGG | - | chr4.1:17605219-17605238 | MS.gene08403:CDS | 40.0% | |
| CTTCCTCCAGAATTTGGATA+TGG | - | chr4.1:17606381-17606400 | MS.gene08403:CDS | 40.0% | |
| CTTGAGCCATATCCAAATTC+TGG | + | chr4.1:17606390-17606409 | None:intergenic | 40.0% | |
| GAATTAACTGTAGCTCCTTC+AGG | - | chr4.1:17605458-17605477 | MS.gene08403:intron | 40.0% | |
| GAATTTGGATATGGCTCAAG+AGG | - | chr4.1:17606390-17606409 | MS.gene08403:CDS | 40.0% | |
| GCTGTTGCAGTAGGTATTAT+TGG | + | chr4.1:17605419-17605438 | None:intergenic | 40.0% | |
| GGAGCTACAGTTAATTCACA+TGG | + | chr4.1:17605455-17605474 | None:intergenic | 40.0% | |
| GTTATCAAAGGATGGGATGT+AGG | - | chr4.1:17606216-17606235 | MS.gene08403:CDS | 40.0% | |
| TCACAATAAGAAAGGCCTGA+AGG | + | chr4.1:17605476-17605495 | None:intergenic | 40.0% | |
| TCACTAGCACCATATTCAGT+TGG | - | chr4.1:17605218-17605237 | MS.gene08403:CDS | 40.0% | |
| TTGAAGCTTCCTCCAGAATT+TGG | - | chr4.1:17606375-17606394 | MS.gene08403:CDS | 40.0% | |
| ! | CTTACATTTCGTGTTGGTGT+TGG | - | chr4.1:17605860-17605879 | MS.gene08403:CDS | 40.0% |
| ! | TCCTTTTGTACAAGAACTGG+TGG | + | chr4.1:17605326-17605345 | None:intergenic | 40.0% |
| ! | TTTTATATCGCGGCTGCAAT+AGG | - | chr4.1:17606014-17606033 | MS.gene08403:intron | 40.0% |
| AAGCGCAATTTCGACTGCAT+TGG | + | chr4.1:17605959-17605978 | None:intergenic | 45.0% | |
| AATTGTAGGCTATGGTCCTC+AGG | - | chr4.1:17605499-17605518 | MS.gene08403:intron | 45.0% | |
| AGGCAAGTCCCAACTGAATA+TGG | + | chr4.1:17605230-17605249 | None:intergenic | 45.0% | |
| CAAGAACTGGTGGTTGTTGT+TGG | + | chr4.1:17605316-17605335 | None:intergenic | 45.0% | |
| GAAGACCAAAACTCAAGGAG+AGG | + | chr4.1:17605379-17605398 | None:intergenic | 45.0% | |
| GAGCCATATCCAAATTCTGG+AGG | + | chr4.1:17606387-17606406 | None:intergenic | 45.0% | |
| GGAATTCCTCCTATGCTTAC+AGG | - | chr4.1:17606255-17606274 | MS.gene08403:CDS | 45.0% | |
| TATGGTCCTCAGGCTGTTAA+GGG | - | chr4.1:17605509-17605528 | MS.gene08403:intron | 45.0% | |
| TATGTGGGGAGATTGGAGAA+TGG | - | chr4.1:17605800-17605819 | MS.gene08403:CDS | 45.0% | |
| TCAAGAGGAGCTGGTTGTAA+AGG | - | chr4.1:17606405-17606424 | MS.gene08403:CDS | 45.0% | |
| ! | ATAGGCCTCTCCTTGAGTTT+TGG | - | chr4.1:17605371-17605390 | MS.gene08403:intron | 45.0% |
| ! | CATTGGACGCATTTTCTCTC+TGG | + | chr4.1:17605942-17605961 | None:intergenic | 45.0% |
| ! | GGATGGGATGTAGGCATTTT+AGG | - | chr4.1:17606225-17606244 | MS.gene08403:CDS | 45.0% |
| ! | TGGGATGTAGGCATTTTAGG+AGG | - | chr4.1:17606228-17606247 | MS.gene08403:CDS | 45.0% |
| !! | ATTTCGTGTTGGTGTTGGAG+AGG | - | chr4.1:17605865-17605884 | MS.gene08403:CDS | 45.0% |
| !! | GTAGGCATTTTAGGAGGTGA+TGG | - | chr4.1:17606234-17606253 | MS.gene08403:CDS | 45.0% |
| !!! | GTTAAGTTTTTGGGGCTGCA+AGG | + | chr4.1:17605258-17605277 | None:intergenic | 45.0% |
| AGAGGAGCTGGTTGTAAAGG+AGG | - | chr4.1:17606408-17606427 | MS.gene08403:CDS | 50.0% | |
| AGTTGTCCCTTAACAGCCTG+AGG | + | chr4.1:17605518-17605537 | None:intergenic | 50.0% | |
| CAGGTACCTGTAAGCATAGG+AGG | + | chr4.1:17606264-17606283 | None:intergenic | 50.0% | |
| CTATGGTCCTCAGGCTGTTA+AGG | - | chr4.1:17605508-17605527 | MS.gene08403:intron | 50.0% | |
| GGCACATTATGTGGGGAGAT+TGG | - | chr4.1:17605793-17605812 | MS.gene08403:CDS | 50.0% | |
| TCGAAATTGCGCTTGTGACG+CGG | - | chr4.1:17605965-17605984 | MS.gene08403:intron | 50.0% | |
| TTCGACAGCAGCTACAATCG+TGG | - | chr4.1:17605830-17605849 | MS.gene08403:CDS | 50.0% | |
| ! | CTGGTGGTTGTTGTTGGACT+TGG | + | chr4.1:17605310-17605329 | None:intergenic | 50.0% |
| !!! | AAGTTTTTGGGGCTGCAAGG+AGG | + | chr4.1:17605255-17605274 | None:intergenic | 50.0% |
| GCTTCAGCTGCTGTTGCAGT+AGG | + | chr4.1:17605428-17605447 | None:intergenic | 55.0% | |
| GGATATGGCTCAAGAGGAGC+TGG | - | chr4.1:17606396-17606415 | MS.gene08403:CDS | 55.0% | |
| ! | TTTGGGGCTGCAAGGAGGTG+AGG | + | chr4.1:17605250-17605269 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 17605212 | 17606467 | 17605212 | ID=MS.gene08403 |
| chr4.1 | mRNA | 17605212 | 17606467 | 17605212 | ID=MS.gene08403.t1;Parent=MS.gene08403 |
| chr4.1 | exon | 17606135 | 17606467 | 17606135 | ID=MS.gene08403.t1.exon1;Parent=MS.gene08403.t1 |
| chr4.1 | CDS | 17606135 | 17606467 | 17606135 | ID=cds.MS.gene08403.t1;Parent=MS.gene08403.t1 |
| chr4.1 | exon | 17605793 | 17605885 | 17605793 | ID=MS.gene08403.t1.exon2;Parent=MS.gene08403.t1 |
| chr4.1 | CDS | 17605793 | 17605885 | 17605793 | ID=cds.MS.gene08403.t1;Parent=MS.gene08403.t1 |
| chr4.1 | exon | 17605403 | 17605463 | 17605403 | ID=MS.gene08403.t1.exon3;Parent=MS.gene08403.t1 |
| chr4.1 | CDS | 17605403 | 17605463 | 17605403 | ID=cds.MS.gene08403.t1;Parent=MS.gene08403.t1 |
| chr4.1 | exon | 17605212 | 17605318 | 17605212 | ID=MS.gene08403.t1.exon4;Parent=MS.gene08403.t1 |
| chr4.1 | CDS | 17605212 | 17605318 | 17605212 | ID=cds.MS.gene08403.t1;Parent=MS.gene08403.t1 |
| Gene Sequence |
| Protein sequence |
