Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25991.t1 | KEH35030.1 | 99.6 | 264 | 1 | 0 | 1 | 264 | 1 | 264 | 5.90E-119 | 437.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25991.t1 | Q9ZSZ8 | 69.5 | 259 | 66 | 3 | 10 | 264 | 7 | 256 | 8.4e-66 | 252.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25991.t1 | A0A072VA17 | 99.6 | 264 | 1 | 0 | 1 | 264 | 1 | 264 | 4.2e-119 | 437.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene25991 | 0.815147 | 1.07E-51 | -1.69E-46 |
| MS.gene04918 | MS.gene25991 | 0.859795 | 3.23E-63 | -1.69E-46 |
| MS.gene04919 | MS.gene25991 | 0.844153 | 9.02E-59 | -1.69E-46 |
| MS.gene04920 | MS.gene25991 | 0.861148 | 1.26E-63 | -1.69E-46 |
| MS.gene049254 | MS.gene25991 | 0.84224 | 2.92E-58 | -1.69E-46 |
| MS.gene049283 | MS.gene25991 | 0.848709 | 5.16E-60 | -1.69E-46 |
| MS.gene049350 | MS.gene25991 | 0.827974 | 1.16E-54 | -1.69E-46 |
| MS.gene049368 | MS.gene25991 | 0.837459 | 5.13E-57 | -1.69E-46 |
| MS.gene049499 | MS.gene25991 | 0.837295 | 5.65E-57 | -1.69E-46 |
| MS.gene049711 | MS.gene25991 | 0.821396 | 4.13E-53 | -1.69E-46 |
| MS.gene049807 | MS.gene25991 | 0.817705 | 2.87E-52 | -1.69E-46 |
| MS.gene049824 | MS.gene25991 | 0.809571 | 1.77E-50 | -1.69E-46 |
| MS.gene049825 | MS.gene25991 | 0.825093 | 5.66E-54 | -1.69E-46 |
| MS.gene049873 | MS.gene25991 | 0.806474 | 8.08E-50 | -1.69E-46 |
| MS.gene049983 | MS.gene25991 | 0.837506 | 4.99E-57 | -1.69E-46 |
| MS.gene050355 | MS.gene25991 | 0.801895 | 7.26E-49 | -1.69E-46 |
| MS.gene050559 | MS.gene25991 | 0.825578 | 4.34E-54 | -1.69E-46 |
| MS.gene050561 | MS.gene25991 | 0.812804 | 3.53E-51 | -1.69E-46 |
| MS.gene050562 | MS.gene25991 | 0.814464 | 1.52E-51 | -1.69E-46 |
| MS.gene050828 | MS.gene25991 | 0.818113 | 2.32E-52 | -1.69E-46 |
| MS.gene050945 | MS.gene25991 | 0.84748 | 1.13E-59 | -1.69E-46 |
| MS.gene051003 | MS.gene25991 | 0.816933 | 4.28E-52 | -1.69E-46 |
| MS.gene051019 | MS.gene25991 | 0.8322 | 1.08E-55 | -1.69E-46 |
| MS.gene051234 | MS.gene25991 | 0.820248 | 7.58E-53 | -1.69E-46 |
| MS.gene051332 | MS.gene25991 | 0.810087 | 1.37E-50 | -1.69E-46 |
| MS.gene051343 | MS.gene25991 | 0.813031 | 3.14E-51 | -1.69E-46 |
| MS.gene051365 | MS.gene25991 | 0.800536 | 1.38E-48 | -1.69E-46 |
| MS.gene051676 | MS.gene25991 | 0.8244 | 8.24E-54 | -1.69E-46 |
| MS.gene051699 | MS.gene25991 | 0.857635 | 1.43E-62 | -1.69E-46 |
| MS.gene051700 | MS.gene25991 | 0.821791 | 3.35E-53 | -1.69E-46 |
| MS.gene051744 | MS.gene25991 | 0.819293 | 1.25E-52 | -1.69E-46 |
| MS.gene051832 | MS.gene25991 | 0.834066 | 3.71E-56 | -1.69E-46 |
| MS.gene05188 | MS.gene25991 | 0.813657 | 2.29E-51 | -1.69E-46 |
| MS.gene051902 | MS.gene25991 | 0.825772 | 3.91E-54 | -1.69E-46 |
| MS.gene051975 | MS.gene25991 | 0.853483 | 2.32E-61 | -1.69E-46 |
| MS.gene052016 | MS.gene25991 | 0.802868 | 4.57E-49 | -1.69E-46 |
| MS.gene052072 | MS.gene25991 | 0.822167 | 2.74E-53 | -1.69E-46 |
| MS.gene052108 | MS.gene25991 | 0.808881 | 2.49E-50 | -1.69E-46 |
| MS.gene052118 | MS.gene25991 | 0.809683 | 1.68E-50 | -1.69E-46 |
| MS.gene052307 | MS.gene25991 | 0.815386 | 9.49E-52 | -1.69E-46 |
| MS.gene052463 | MS.gene25991 | 0.829166 | 5.99E-55 | -1.69E-46 |
| MS.gene052518 | MS.gene25991 | 0.824272 | 8.83E-54 | -1.69E-46 |
| MS.gene052519 | MS.gene25991 | 0.826927 | 2.07E-54 | -1.69E-46 |
| MS.gene052533 | MS.gene25991 | 0.850559 | 1.57E-60 | -1.69E-46 |
| MS.gene052582 | MS.gene25991 | 0.826068 | 3.32E-54 | -1.69E-46 |
| MS.gene052584 | MS.gene25991 | 0.833615 | 4.82E-56 | -1.69E-46 |
| MS.gene052685 | MS.gene25991 | 0.836697 | 8.04E-57 | -1.69E-46 |
| MS.gene052717 | MS.gene25991 | 0.809271 | 2.05E-50 | -1.69E-46 |
| MS.gene052773 | MS.gene25991 | 0.802729 | 4.89E-49 | -1.69E-46 |
| MS.gene052793 | MS.gene25991 | 0.834213 | 3.41E-56 | -1.69E-46 |
| MS.gene052889 | MS.gene25991 | 0.810186 | 1.31E-50 | -1.69E-46 |
| MS.gene052947 | MS.gene25991 | 0.800769 | 1.23E-48 | -1.69E-46 |
| MS.gene053079 | MS.gene25991 | 0.810247 | 1.27E-50 | -1.69E-46 |
| MS.gene053122 | MS.gene25991 | 0.820322 | 7.29E-53 | -1.69E-46 |
| MS.gene053226 | MS.gene25991 | 0.837468 | 5.11E-57 | -1.69E-46 |
| MS.gene053272 | MS.gene25991 | -0.824646 | 7.21E-54 | -1.69E-46 |
| MS.gene053298 | MS.gene25991 | 0.840142 | 1.04E-57 | -1.69E-46 |
| MS.gene053599 | MS.gene25991 | 0.803005 | 4.29E-49 | -1.69E-46 |
| MS.gene05364 | MS.gene25991 | 0.821284 | 4.38E-53 | -1.69E-46 |
| MS.gene05365 | MS.gene25991 | 0.822222 | 2.66E-53 | -1.69E-46 |
| MS.gene053931 | MS.gene25991 | 0.811628 | 6.36E-51 | -1.69E-46 |
| MS.gene053963 | MS.gene25991 | 0.838371 | 2.99E-57 | -1.69E-46 |
| MS.gene054142 | MS.gene25991 | 0.818954 | 1.50E-52 | -1.69E-46 |
| MS.gene054178 | MS.gene25991 | 0.815668 | 8.22E-52 | -1.69E-46 |
| MS.gene054222 | MS.gene25991 | 0.843053 | 1.78E-58 | -1.69E-46 |
| MS.gene05423 | MS.gene25991 | 0.814378 | 1.59E-51 | -1.69E-46 |
| MS.gene05458 | MS.gene25991 | 0.833818 | 4.29E-56 | -1.69E-46 |
| MS.gene054651 | MS.gene25991 | 0.824565 | 7.53E-54 | -1.69E-46 |
| MS.gene054892 | MS.gene25991 | 0.82578 | 3.89E-54 | -1.69E-46 |
| MS.gene054893 | MS.gene25991 | 0.826023 | 3.40E-54 | -1.69E-46 |
| MS.gene054957 | MS.gene25991 | 0.831029 | 2.10E-55 | -1.69E-46 |
| MS.gene055229 | MS.gene25991 | 0.804544 | 2.05E-49 | -1.69E-46 |
| MS.gene055513 | MS.gene25991 | 0.832819 | 7.60E-56 | -1.69E-46 |
| MS.gene055555 | MS.gene25991 | 0.808352 | 3.23E-50 | -1.69E-46 |
| MS.gene055586 | MS.gene25991 | 0.805295 | 1.43E-49 | -1.69E-46 |
| MS.gene055694 | MS.gene25991 | 0.801073 | 1.07E-48 | -1.69E-46 |
| MS.gene055801 | MS.gene25991 | 0.812738 | 3.65E-51 | -1.69E-46 |
| MS.gene055808 | MS.gene25991 | 0.806173 | 9.35E-50 | -1.69E-46 |
| MS.gene056082 | MS.gene25991 | 0.820837 | 5.55E-53 | -1.69E-46 |
| MS.gene056174 | MS.gene25991 | 0.801644 | 8.17E-49 | -1.69E-46 |
| MS.gene056215 | MS.gene25991 | 0.824348 | 8.48E-54 | -1.69E-46 |
| MS.gene05636 | MS.gene25991 | 0.819465 | 1.15E-52 | -1.69E-46 |
| MS.gene056403 | MS.gene25991 | 0.800389 | 1.47E-48 | -1.69E-46 |
| MS.gene056699 | MS.gene25991 | 0.809397 | 1.93E-50 | -1.69E-46 |
| MS.gene056776 | MS.gene25991 | 0.839423 | 1.60E-57 | -1.69E-46 |
| MS.gene05686 | MS.gene25991 | 0.853276 | 2.66E-61 | -1.69E-46 |
| MS.gene05689 | MS.gene25991 | 0.843106 | 1.72E-58 | -1.69E-46 |
| MS.gene05690 | MS.gene25991 | 0.82813 | 1.07E-54 | -1.69E-46 |
| MS.gene056986 | MS.gene25991 | 0.858026 | 1.09E-62 | -1.69E-46 |
| MS.gene057102 | MS.gene25991 | 0.910169 | 2.53E-82 | -1.69E-46 |
| MS.gene057208 | MS.gene25991 | 0.85028 | 1.88E-60 | -1.69E-46 |
| MS.gene057598 | MS.gene25991 | 0.805195 | 1.50E-49 | -1.69E-46 |
| MS.gene057611 | MS.gene25991 | 0.804298 | 2.31E-49 | -1.69E-46 |
| MS.gene057890 | MS.gene25991 | -0.825256 | 5.17E-54 | -1.69E-46 |
| MS.gene057893 | MS.gene25991 | -0.839714 | 1.34E-57 | -1.69E-46 |
| MS.gene058008 | MS.gene25991 | 0.82612 | 3.23E-54 | -1.69E-46 |
| MS.gene058009 | MS.gene25991 | 0.805274 | 1.44E-49 | -1.69E-46 |
| MS.gene058037 | MS.gene25991 | 0.811993 | 5.30E-51 | -1.69E-46 |
| MS.gene058149 | MS.gene25991 | 0.804903 | 1.73E-49 | -1.69E-46 |
| MS.gene058159 | MS.gene25991 | 0.853558 | 2.21E-61 | -1.69E-46 |
| MS.gene058279 | MS.gene25991 | 0.812903 | 3.35E-51 | -1.69E-46 |
| MS.gene058330 | MS.gene25991 | 0.803324 | 3.68E-49 | -1.69E-46 |
| MS.gene058355 | MS.gene25991 | 0.814565 | 1.44E-51 | -1.69E-46 |
| MS.gene058592 | MS.gene25991 | 0.81574 | 7.92E-52 | -1.69E-46 |
| MS.gene058625 | MS.gene25991 | 0.80327 | 3.78E-49 | -1.69E-46 |
| MS.gene058781 | MS.gene25991 | 0.814991 | 1.16E-51 | -1.69E-46 |
| MS.gene058783 | MS.gene25991 | 0.818407 | 1.99E-52 | -1.69E-46 |
| MS.gene05878 | MS.gene25991 | 0.800573 | 1.35E-48 | -1.69E-46 |
| MS.gene059146 | MS.gene25991 | 0.856952 | 2.27E-62 | -1.69E-46 |
| MS.gene059170 | MS.gene25991 | 0.817122 | 3.88E-52 | -1.69E-46 |
| MS.gene059227 | MS.gene25991 | 0.826877 | 2.13E-54 | -1.69E-46 |
| MS.gene05952 | MS.gene25991 | 0.840494 | 8.40E-58 | -1.69E-46 |
| MS.gene05954 | MS.gene25991 | 0.800583 | 1.35E-48 | -1.69E-46 |
| MS.gene059625 | MS.gene25991 | 0.821146 | 4.71E-53 | -1.69E-46 |
| MS.gene059778 | MS.gene25991 | 0.810975 | 8.82E-51 | -1.69E-46 |
| MS.gene05985 | MS.gene25991 | -0.882763 | 7.93E-71 | -1.69E-46 |
| MS.gene059901 | MS.gene25991 | -0.835859 | 1.31E-56 | -1.69E-46 |
| MS.gene059935 | MS.gene25991 | 0.832433 | 9.48E-56 | -1.69E-46 |
| MS.gene06011 | MS.gene25991 | 0.816581 | 5.14E-52 | -1.69E-46 |
| MS.gene060259 | MS.gene25991 | 0.825345 | 4.93E-54 | -1.69E-46 |
| MS.gene060338 | MS.gene25991 | 0.815583 | 8.58E-52 | -1.69E-46 |
| MS.gene060365 | MS.gene25991 | 0.85368 | 2.04E-61 | -1.69E-46 |
| MS.gene060429 | MS.gene25991 | 0.802644 | 5.09E-49 | -1.69E-46 |
| MS.gene060435 | MS.gene25991 | 0.813337 | 2.69E-51 | -1.69E-46 |
| MS.gene060512 | MS.gene25991 | 0.806308 | 8.76E-50 | -1.69E-46 |
| MS.gene060521 | MS.gene25991 | 0.814092 | 1.84E-51 | -1.69E-46 |
| MS.gene060763 | MS.gene25991 | 0.817121 | 3.89E-52 | -1.69E-46 |
| MS.gene060933 | MS.gene25991 | 0.836838 | 7.40E-57 | -1.69E-46 |
| MS.gene060935 | MS.gene25991 | 0.870805 | 1.11E-66 | -1.69E-46 |
| MS.gene060937 | MS.gene25991 | 0.878532 | 2.60E-69 | -1.69E-46 |
| MS.gene061100 | MS.gene25991 | 0.849645 | 2.83E-60 | -1.69E-46 |
| MS.gene061160 | MS.gene25991 | 0.849431 | 3.25E-60 | -1.69E-46 |
| MS.gene061188 | MS.gene25991 | 0.844386 | 7.81E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25991.t1 | MTR_3g077320 | 96.524 | 374 | 9 | 1 | 1 | 370 | 1 | 374 | 0.0 | 735 |
| MS.gene25991.t1 | MTR_3g077320 | 99.259 | 270 | 2 | 0 | 1 | 270 | 1 | 270 | 0.0 | 558 |
| MS.gene25991.t1 | MTR_0325s0020 | 68.770 | 317 | 94 | 3 | 10 | 322 | 209 | 524 | 2.30e-147 | 427 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25991.t1 | AT3G54170 | 69.097 | 288 | 74 | 4 | 10 | 292 | 7 | 284 | 1.09e-133 | 385 |
Find 94 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGACTCTTCGGATGATTTC+AGG | 0.161909 | 3.3:+65650516 | None:intergenic |
| TTCGATTTCGGAGGTGAAAT+TGG | 0.256929 | 3.3:-65652944 | MS.gene25991:CDS |
| GAAATTGGTGGAAGGCATTC+AGG | 0.288629 | 3.3:-65652929 | MS.gene25991:intron |
| CATCTTCCCCATCTTGCTTT+GGG | 0.292814 | 3.3:+65648032 | None:intergenic |
| CATTAAAACATTTGCAAAAC+GGG | 0.325057 | 3.3:-65650357 | MS.gene25991:CDS |
| GCATCTTCCCCATCTTGCTT+TGG | 0.337652 | 3.3:+65648031 | None:intergenic |
| AGTTTCTTCAACTTTAGATT+TGG | 0.349237 | 3.3:+65651371 | None:intergenic |
| AATCAATCAATTACCTTCTT+CGG | 0.359272 | 3.3:+65652772 | None:intergenic |
| GTTTCAGTCTTTGAATCTCC+TGG | 0.375507 | 3.3:+65646198 | None:intergenic |
| TAATCTTCGTCGTCGTAATC+CGG | 0.402595 | 3.3:+65652814 | None:intergenic |
| TTACATCGTCGAAACGAGTT+GGG | 0.405362 | 3.3:+65653049 | None:intergenic |
| TATAAGCATACCTGCATTGA+TGG | 0.414521 | 3.3:+65649519 | None:intergenic |
| AATGTTGATGAATTATCTTC+AGG | 0.414876 | 3.3:-65650540 | MS.gene25991:CDS |
| GTATGTGCTGGGGCTACTCC+AGG | 0.422901 | 3.3:-65650707 | MS.gene25991:intron |
| TGAAGAGTTCACACGTTTGA+AGG | 0.425230 | 3.3:-65648838 | MS.gene25991:intron |
| GGCGGTGACTGAGGAGGATA+AGG | 0.427854 | 3.3:-65646051 | MS.gene25991:CDS |
| GACGGTGGCTGAGGAGGATA+AGG | 0.427854 | 3.3:-65646081 | MS.gene25991:CDS |
| GCTTTGGGAAGTAAATGTTA+TGG | 0.432063 | 3.3:+65648047 | None:intergenic |
| CAACTCAGAAGTCAATTTGA+AGG | 0.433565 | 3.3:-65647685 | MS.gene25991:intron |
| CTGCTCTCTCAATGACTCTT+CGG | 0.440775 | 3.3:+65650505 | None:intergenic |
| TGACTCTTCGGATGATTTCA+GGG | 0.444705 | 3.3:+65650517 | None:intergenic |
| AAACTTTCAGTCTGCTGTGC+GGG | 0.448301 | 3.3:-65649565 | MS.gene25991:intron |
| ATTACCTTCTTCGGTGCAAA+AGG | 0.449253 | 3.3:+65652781 | None:intergenic |
| CAAGAAGAAAACGAGGAGAT+TGG | 0.450411 | 3.3:-65647985 | MS.gene25991:CDS |
| TCATTAAAACATTTGCAAAA+CGG | 0.457616 | 3.3:-65650358 | MS.gene25991:CDS |
| TACAGGGTTGCAGAAGCATA+TGG | 0.468685 | 3.3:-65647156 | MS.gene25991:intron |
| TTGCAGCCTCCAGAGCTGTA+AGG | 0.469485 | 3.3:-65651211 | MS.gene25991:intron |
| TTTACTTCCCAAAGCAAGAT+GGG | 0.471815 | 3.3:-65648039 | MS.gene25991:CDS |
| CTCATCCTATCAGAATTTAG+TGG | 0.474105 | 3.3:-65648115 | MS.gene25991:intron |
| AATGAGCCTGTTGTATGTGC+TGG | 0.474348 | 3.3:-65650719 | MS.gene25991:CDS |
| AAGGAGGTTGTTACTAGATC+CGG | 0.480093 | 3.3:-65648868 | MS.gene25991:CDS |
| TACTGGTTGTCAGCTTGAAA+AGG | 0.490273 | 3.3:-65650402 | MS.gene25991:intron |
| GTAGCCCCAGCACATACAAC+AGG | 0.493156 | 3.3:+65650713 | None:intergenic |
| CAAGCCGATCCTTACAGCTC+TGG | 0.493375 | 3.3:+65651202 | None:intergenic |
| CGAAGAGTCATTGAGAGAGC+AGG | 0.497852 | 3.3:-65650504 | MS.gene25991:intron |
| ATGAGCCTGTTGTATGTGCT+GGG | 0.502100 | 3.3:-65650718 | MS.gene25991:CDS |
| ACTGCCTCTGGTGTAACAAC+AGG | 0.502296 | 3.3:-65651351 | MS.gene25991:CDS |
| ATTTACTTCCCAAAGCAAGA+TGG | 0.502506 | 3.3:-65648040 | MS.gene25991:CDS |
| AAATTAATTCGCAGCCTCCT+TGG | 0.503053 | 3.3:+65646001 | None:intergenic |
| GAAGCTAGACGAGAAAGGCC+AGG | 0.503947 | 3.3:-65646216 | MS.gene25991:CDS |
| CTTACATCGTCGAAACGAGT+TGG | 0.509237 | 3.3:+65653048 | None:intergenic |
| GGATTTGCAAGATAACATTA+CGG | 0.511867 | 3.3:-65648070 | MS.gene25991:CDS |
| TGAGGAGGATAAGGAGGATA+AGG | 0.515579 | 3.3:-65646072 | MS.gene25991:CDS |
| CAAAAGAACTTAGCGGAGGA+TGG | 0.520918 | 3.3:-65646164 | MS.gene25991:CDS |
| CAGTGATAAGACGGTGGCTG+AGG | 0.530018 | 3.3:-65646111 | MS.gene25991:CDS |
| CAAGAGAAGCTAGACGAGAA+AGG | 0.541633 | 3.3:-65646221 | MS.gene25991:CDS |
| AAAGTTGAAGAAACTGCCTC+TGG | 0.544941 | 3.3:-65651363 | MS.gene25991:CDS |
| AGAACTTAGCGGAGGATGGA+AGG | 0.546938 | 3.3:-65646160 | MS.gene25991:CDS |
| GGAGGATAAGACGGTGGCTG+AGG | 0.547486 | 3.3:-65646090 | MS.gene25991:CDS |
| ACTCAAGCCGCCATCAATGC+AGG | 0.552351 | 3.3:-65649529 | MS.gene25991:intron |
| TGATAAGACGGTGGCTGAGG+AGG | 0.559728 | 3.3:-65646108 | MS.gene25991:CDS |
| GGATAAGACGGTGGCTGAGG+AGG | 0.560217 | 3.3:-65646087 | MS.gene25991:CDS |
| CAAAGTCTGAGATTCATAGT+TGG | 0.562090 | 3.3:-65650757 | MS.gene25991:CDS |
| TCAGAATTTAGTGGAGGAGA+AGG | 0.567099 | 3.3:-65648106 | MS.gene25991:intron |
| GATTTCGGAGGTGAAATTGG+TGG | 0.567451 | 3.3:-65652941 | MS.gene25991:CDS |
| GTGAACTCTTCATGAACAGC+CGG | 0.571799 | 3.3:+65648849 | None:intergenic |
| TCGTCGTCGTAATCCGGCGA+TGG | 0.576592 | 3.3:+65652820 | None:intergenic |
| AAATCAAGCTTCTGAAGGAA+AGG | 0.576681 | 3.3:-65647962 | MS.gene25991:intron |
| GCCTCCAGAGCTGTAAGGAT+CGG | 0.577211 | 3.3:-65651206 | MS.gene25991:CDS |
| TGTGGAGAGATCAAATGAAA+CGG | 0.578417 | 3.3:-65647117 | MS.gene25991:intron |
| GTATTTCCAGAGTGAGCTTG+AGG | 0.596741 | 3.3:-65650783 | MS.gene25991:intron |
| GCCGATCCTTACAGCTCTGG+AGG | 0.599157 | 3.3:+65651205 | None:intergenic |
| GGAGGATAAGACAGTGACCA+AGG | 0.603085 | 3.3:-65646018 | MS.gene25991:CDS |
| GGAGGATAAGGCGGTGACTG+AGG | 0.608632 | 3.3:-65646060 | MS.gene25991:CDS |
| TCGGAGGTGAAATTGGTGGA+AGG | 0.608878 | 3.3:-65652937 | MS.gene25991:CDS |
| ACTCCAGCAAAAGAACTTAG+CGG | 0.619444 | 3.3:-65646171 | MS.gene25991:CDS |
| TACATCGTCGAAACGAGTTG+GGG | 0.620919 | 3.3:+65653050 | None:intergenic |
| TAAGAGTGACAGTGATAAGA+CGG | 0.623426 | 3.3:-65646120 | MS.gene25991:CDS |
| ATTGGAAATCAAGCTTCTGA+AGG | 0.624930 | 3.3:-65647967 | MS.gene25991:CDS |
| GGAGGATAAGGCGGTGACTG+AGG | 0.626582 | 3.3:-65646039 | MS.gene25991:CDS |
| GGATAAGGCGGTGACTGAGG+AGG | 0.629527 | 3.3:-65646036 | MS.gene25991:CDS |
| GGATAAGGCGGTGACTGAGG+AGG | 0.629527 | 3.3:-65646057 | MS.gene25991:CDS |
| CATTCCTGTTGTTACACCAG+AGG | 0.631750 | 3.3:+65651347 | None:intergenic |
| GGTGGCTGAGGAGGATAAGA+CGG | 0.632347 | 3.3:-65646099 | MS.gene25991:CDS |
| TCACTGTCACTCTTACTATG+TGG | 0.638863 | 3.3:+65646128 | None:intergenic |
| GGTGGCTGAGGAGGATAAGG+AGG | 0.640276 | 3.3:-65646078 | MS.gene25991:CDS |
| TGATCTTGTCACTTCGTGAG+AGG | 0.642970 | 3.3:-65651326 | MS.gene25991:intron |
| CAAGATGGGGAAGATGCTGA+TGG | 0.643010 | 3.3:-65648025 | MS.gene25991:CDS |
| GAAACTTTCAGTCTGCTGTG+CGG | 0.644244 | 3.3:-65649566 | MS.gene25991:intron |
| AAGCATACCTGCATTGATGG+CGG | 0.659147 | 3.3:+65649522 | None:intergenic |
| GGGTTGCAGAAGCATATGGA+AGG | 0.661064 | 3.3:-65647152 | MS.gene25991:CDS |
| ATCCTATCAGAATTTAGTGG+AGG | 0.662269 | 3.3:-65648112 | MS.gene25991:intron |
| AAAACGGGAGCAAGAGATAG+CGG | 0.663083 | 3.3:-65650342 | MS.gene25991:CDS |
| TTACTTCCCAAAGCAAGATG+GGG | 0.664236 | 3.3:-65648038 | MS.gene25991:CDS |
| GGAAGGACTGACAAATGATG+TGG | 0.666612 | 3.3:-65647135 | MS.gene25991:CDS |
| AGGTAATAAGAGACCATCGC+CGG | 0.668265 | 3.3:-65652833 | MS.gene25991:CDS |
| AACCCTGCAAGAAGAAAACG+AGG | 0.669091 | 3.3:-65647992 | MS.gene25991:CDS |
| CCAGCAAAAGAACTTAGCGG+AGG | 0.696105 | 3.3:-65646168 | MS.gene25991:CDS |
| GGAGGATAAGGAGGATAAGG+CGG | 0.697058 | 3.3:-65646069 | MS.gene25991:CDS |
| GGTGACTGAGGAGGATAAGG+CGG | 0.699747 | 3.3:-65646048 | MS.gene25991:CDS |
| GGCTGAGGAGGATAAGACGG+TGG | 0.703854 | 3.3:-65646096 | MS.gene25991:CDS |
| TGAGCCTGTTGTATGTGCTG+GGG | 0.705363 | 3.3:-65650717 | MS.gene25991:CDS |
| GGATAAGACAGTGACCAAGG+AGG | 0.735662 | 3.3:-65646015 | MS.gene25991:CDS |
| GAGTGACAGTGATAAGACGG+TGG | 0.746274 | 3.3:-65646117 | MS.gene25991:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 65646004 | 65653079 | 65646004 | ID=MS.gene25991 |
| chr3.3 | mRNA | 65646004 | 65653079 | 65646004 | ID=MS.gene25991.t1;Parent=MS.gene25991 |
| chr3.3 | exon | 65653053 | 65653079 | 65653053 | ID=MS.gene25991.t1.exon1;Parent=MS.gene25991.t1 |
| chr3.3 | CDS | 65653053 | 65653079 | 65653053 | ID=cds.MS.gene25991.t1;Parent=MS.gene25991.t1 |
| chr3.3 | exon | 65652930 | 65652969 | 65652930 | ID=MS.gene25991.t1.exon2;Parent=MS.gene25991.t1 |
| chr3.3 | CDS | 65652930 | 65652969 | 65652930 | ID=cds.MS.gene25991.t1;Parent=MS.gene25991.t1 |
| chr3.3 | exon | 65652786 | 65652853 | 65652786 | ID=MS.gene25991.t1.exon3;Parent=MS.gene25991.t1 |
| chr3.3 | CDS | 65652786 | 65652853 | 65652786 | ID=cds.MS.gene25991.t1;Parent=MS.gene25991.t1 |
| chr3.3 | exon | 65651327 | 65651394 | 65651327 | ID=MS.gene25991.t1.exon4;Parent=MS.gene25991.t1 |
| chr3.3 | CDS | 65651327 | 65651394 | 65651327 | ID=cds.MS.gene25991.t1;Parent=MS.gene25991.t1 |
| chr3.3 | exon | 65651191 | 65651227 | 65651191 | ID=MS.gene25991.t1.exon5;Parent=MS.gene25991.t1 |
| chr3.3 | CDS | 65651191 | 65651227 | 65651191 | ID=cds.MS.gene25991.t1;Parent=MS.gene25991.t1 |
| chr3.3 | exon | 65650708 | 65650795 | 65650708 | ID=MS.gene25991.t1.exon6;Parent=MS.gene25991.t1 |
| chr3.3 | CDS | 65650708 | 65650795 | 65650708 | ID=cds.MS.gene25991.t1;Parent=MS.gene25991.t1 |
| chr3.3 | exon | 65650505 | 65650569 | 65650505 | ID=MS.gene25991.t1.exon7;Parent=MS.gene25991.t1 |
| chr3.3 | CDS | 65650505 | 65650569 | 65650505 | ID=cds.MS.gene25991.t1;Parent=MS.gene25991.t1 |
| chr3.3 | exon | 65650334 | 65650411 | 65650334 | ID=MS.gene25991.t1.exon8;Parent=MS.gene25991.t1 |
| chr3.3 | CDS | 65650334 | 65650411 | 65650334 | ID=cds.MS.gene25991.t1;Parent=MS.gene25991.t1 |
| chr3.3 | exon | 65649530 | 65649577 | 65649530 | ID=MS.gene25991.t1.exon9;Parent=MS.gene25991.t1 |
| chr3.3 | CDS | 65649530 | 65649577 | 65649530 | ID=cds.MS.gene25991.t1;Parent=MS.gene25991.t1 |
| chr3.3 | exon | 65648839 | 65648892 | 65648839 | ID=MS.gene25991.t1.exon10;Parent=MS.gene25991.t1 |
| chr3.3 | CDS | 65648839 | 65648892 | 65648839 | ID=cds.MS.gene25991.t1;Parent=MS.gene25991.t1 |
| chr3.3 | exon | 65647963 | 65648124 | 65647963 | ID=MS.gene25991.t1.exon11;Parent=MS.gene25991.t1 |
| chr3.3 | CDS | 65647963 | 65648124 | 65647963 | ID=cds.MS.gene25991.t1;Parent=MS.gene25991.t1 |
| chr3.3 | exon | 65647686 | 65647755 | 65647686 | ID=MS.gene25991.t1.exon12;Parent=MS.gene25991.t1 |
| chr3.3 | CDS | 65647686 | 65647755 | 65647686 | ID=cds.MS.gene25991.t1;Parent=MS.gene25991.t1 |
| chr3.3 | exon | 65647118 | 65647173 | 65647118 | ID=MS.gene25991.t1.exon13;Parent=MS.gene25991.t1 |
| chr3.3 | CDS | 65647118 | 65647173 | 65647118 | ID=cds.MS.gene25991.t1;Parent=MS.gene25991.t1 |
| chr3.3 | exon | 65646004 | 65646255 | 65646004 | ID=MS.gene25991.t1.exon14;Parent=MS.gene25991.t1 |
| chr3.3 | CDS | 65646004 | 65646255 | 65646004 | ID=cds.MS.gene25991.t1;Parent=MS.gene25991.t1 |
| Gene Sequence |
| Protein sequence |
