Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60402.t1 | KEH31552.1 | 100 | 43 | 0 | 0 | 1 | 43 | 1 | 43 | 6.30E-15 | 90.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60402.t1 | Q93ZB1 | 100.0 | 39 | 0 | 0 | 5 | 43 | 32 | 70 | 1.4e-15 | 84.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60402.t1 | A0A072UPD4 | 100.0 | 43 | 0 | 0 | 1 | 43 | 1 | 43 | 4.5e-15 | 90.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene60402.t1 | TF | C2C2-LSD |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene60402 | 0.831127 | 1.99E-55 | -1.69E-46 |
| MS.gene049173 | MS.gene60402 | 0.821572 | 3.76E-53 | -1.69E-46 |
| MS.gene049217 | MS.gene60402 | 0.884058 | 2.65E-71 | -1.69E-46 |
| MS.gene049222 | MS.gene60402 | 0.834188 | 3.46E-56 | -1.69E-46 |
| MS.gene049285 | MS.gene60402 | 0.884105 | 2.55E-71 | -1.69E-46 |
| MS.gene049320 | MS.gene60402 | 0.877838 | 4.56E-69 | -1.69E-46 |
| MS.gene049427 | MS.gene60402 | 0.829531 | 4.89E-55 | -1.69E-46 |
| MS.gene049609 | MS.gene60402 | 0.811897 | 5.56E-51 | -1.69E-46 |
| MS.gene049614 | MS.gene60402 | 0.831566 | 1.55E-55 | -1.69E-46 |
| MS.gene049627 | MS.gene60402 | 0.820468 | 6.75E-53 | -1.69E-46 |
| MS.gene050076 | MS.gene60402 | 0.809621 | 1.73E-50 | -1.69E-46 |
| MS.gene05010 | MS.gene60402 | 0.811692 | 6.17E-51 | -1.69E-46 |
| MS.gene050163 | MS.gene60402 | 0.89116 | 5.12E-74 | -1.69E-46 |
| MS.gene050165 | MS.gene60402 | 0.878147 | 3.55E-69 | -1.69E-46 |
| MS.gene050333 | MS.gene60402 | 0.840623 | 7.78E-58 | -1.69E-46 |
| MS.gene050353 | MS.gene60402 | -0.832987 | 6.91E-56 | -1.69E-46 |
| MS.gene050432 | MS.gene60402 | 0.819781 | 9.70E-53 | -1.69E-46 |
| MS.gene050504 | MS.gene60402 | 0.852835 | 3.56E-61 | -1.69E-46 |
| MS.gene050505 | MS.gene60402 | -0.80982 | 1.57E-50 | -1.69E-46 |
| MS.gene050518 | MS.gene60402 | 0.841745 | 3.94E-58 | -1.69E-46 |
| MS.gene050612 | MS.gene60402 | 0.860401 | 2.12E-63 | -1.69E-46 |
| MS.gene050675 | MS.gene60402 | 0.821197 | 4.59E-53 | -1.69E-46 |
| MS.gene050699 | MS.gene60402 | 0.855683 | 5.35E-62 | -1.69E-46 |
| MS.gene050767 | MS.gene60402 | 0.849901 | 2.40E-60 | -1.69E-46 |
| MS.gene050770 | MS.gene60402 | 0.853869 | 1.80E-61 | -1.69E-46 |
| MS.gene050772 | MS.gene60402 | 0.848839 | 4.75E-60 | -1.69E-46 |
| MS.gene050986 | MS.gene60402 | 0.843255 | 1.57E-58 | -1.69E-46 |
| MS.gene05100 | MS.gene60402 | 0.863567 | 2.27E-64 | -1.69E-46 |
| MS.gene051228 | MS.gene60402 | 0.835701 | 1.44E-56 | -1.69E-46 |
| MS.gene051434 | MS.gene60402 | 0.821124 | 4.77E-53 | -1.69E-46 |
| MS.gene051551 | MS.gene60402 | 0.821221 | 4.53E-53 | -1.69E-46 |
| MS.gene051564 | MS.gene60402 | 0.808058 | 3.73E-50 | -1.69E-46 |
| MS.gene051566 | MS.gene60402 | 0.81514 | 1.08E-51 | -1.69E-46 |
| MS.gene051692 | MS.gene60402 | 0.801638 | 8.19E-49 | -1.69E-46 |
| MS.gene051694 | MS.gene60402 | 0.834013 | 3.83E-56 | -1.69E-46 |
| MS.gene051810 | MS.gene60402 | 0.902594 | 8.26E-79 | -1.69E-46 |
| MS.gene051858 | MS.gene60402 | 0.861645 | 8.87E-64 | -1.69E-46 |
| MS.gene051949 | MS.gene60402 | 0.834783 | 2.45E-56 | -1.69E-46 |
| MS.gene052271 | MS.gene60402 | 0.893517 | 5.84E-75 | -1.69E-46 |
| MS.gene052379 | MS.gene60402 | 0.8743 | 7.52E-68 | -1.69E-46 |
| MS.gene052484 | MS.gene60402 | 0.813296 | 2.75E-51 | -1.69E-46 |
| MS.gene052615 | MS.gene60402 | 0.823172 | 1.60E-53 | -1.69E-46 |
| MS.gene052870 | MS.gene60402 | 0.80633 | 8.67E-50 | -1.69E-46 |
| MS.gene052872 | MS.gene60402 | 0.817735 | 2.83E-52 | -1.69E-46 |
| MS.gene053009 | MS.gene60402 | 0.818479 | 1.92E-52 | -1.69E-46 |
| MS.gene053104 | MS.gene60402 | 0.819529 | 1.11E-52 | -1.69E-46 |
| MS.gene053107 | MS.gene60402 | 0.804496 | 2.10E-49 | -1.69E-46 |
| MS.gene053207 | MS.gene60402 | 0.819229 | 1.30E-52 | -1.69E-46 |
| MS.gene053213 | MS.gene60402 | 0.8714 | 7.03E-67 | -1.69E-46 |
| MS.gene053286 | MS.gene60402 | 0.867911 | 9.65E-66 | -1.69E-46 |
| MS.gene053322 | MS.gene60402 | 0.873053 | 1.98E-67 | -1.69E-46 |
| MS.gene053561 | MS.gene60402 | -0.820915 | 5.33E-53 | -1.69E-46 |
| MS.gene053632 | MS.gene60402 | 0.84184 | 3.72E-58 | -1.69E-46 |
| MS.gene053915 | MS.gene60402 | 0.802249 | 6.14E-49 | -1.69E-46 |
| MS.gene054376 | MS.gene60402 | 0.836964 | 6.87E-57 | -1.69E-46 |
| MS.gene054600 | MS.gene60402 | 0.838012 | 3.70E-57 | -1.69E-46 |
| MS.gene054642 | MS.gene60402 | 0.890557 | 8.85E-74 | -1.69E-46 |
| MS.gene054802 | MS.gene60402 | 0.850503 | 1.63E-60 | -1.69E-46 |
| MS.gene054803 | MS.gene60402 | 0.844399 | 7.75E-59 | -1.69E-46 |
| MS.gene054804 | MS.gene60402 | 0.851629 | 7.83E-61 | -1.69E-46 |
| MS.gene054954 | MS.gene60402 | 0.857035 | 2.15E-62 | -1.69E-46 |
| MS.gene055154 | MS.gene60402 | -0.80983 | 1.56E-50 | -1.69E-46 |
| MS.gene055206 | MS.gene60402 | 0.848988 | 4.31E-60 | -1.69E-46 |
| MS.gene055212 | MS.gene60402 | 0.831999 | 1.21E-55 | -1.69E-46 |
| MS.gene055254 | MS.gene60402 | 0.830795 | 2.40E-55 | -1.69E-46 |
| MS.gene055289 | MS.gene60402 | 0.808098 | 3.66E-50 | -1.69E-46 |
| MS.gene055362 | MS.gene60402 | 0.879701 | 1.01E-69 | -1.69E-46 |
| MS.gene055397 | MS.gene60402 | 0.857608 | 1.45E-62 | -1.69E-46 |
| MS.gene055499 | MS.gene60402 | 0.882925 | 6.92E-71 | -1.69E-46 |
| MS.gene055502 | MS.gene60402 | 0.842889 | 1.96E-58 | -1.69E-46 |
| MS.gene055577 | MS.gene60402 | 0.828685 | 7.83E-55 | -1.69E-46 |
| MS.gene055636 | MS.gene60402 | 0.844971 | 5.43E-59 | -1.69E-46 |
| MS.gene055638 | MS.gene60402 | 0.838028 | 3.67E-57 | -1.69E-46 |
| MS.gene055647 | MS.gene60402 | 0.848341 | 6.52E-60 | -1.69E-46 |
| MS.gene055722 | MS.gene60402 | 0.845715 | 3.42E-59 | -1.69E-46 |
| MS.gene05592 | MS.gene60402 | 0.836421 | 9.45E-57 | -1.69E-46 |
| MS.gene055941 | MS.gene60402 | 0.822464 | 2.34E-53 | -1.69E-46 |
| MS.gene055956 | MS.gene60402 | -0.847952 | 8.35E-60 | -1.69E-46 |
| MS.gene056083 | MS.gene60402 | 0.833936 | 4.00E-56 | -1.69E-46 |
| MS.gene056374 | MS.gene60402 | 0.889169 | 3.08E-73 | -1.69E-46 |
| MS.gene056504 | MS.gene60402 | 0.808196 | 3.49E-50 | -1.69E-46 |
| MS.gene056510 | MS.gene60402 | 0.810237 | 1.27E-50 | -1.69E-46 |
| MS.gene056549 | MS.gene60402 | 0.81431 | 1.64E-51 | -1.69E-46 |
| MS.gene056550 | MS.gene60402 | -0.810734 | 9.95E-51 | -1.69E-46 |
| MS.gene056657 | MS.gene60402 | 0.880362 | 5.84E-70 | -1.69E-46 |
| MS.gene056692 | MS.gene60402 | 0.809618 | 1.73E-50 | -1.69E-46 |
| MS.gene056946 | MS.gene60402 | 0.809042 | 2.30E-50 | -1.69E-46 |
| MS.gene057011 | MS.gene60402 | 0.818509 | 1.89E-52 | -1.69E-46 |
| MS.gene057129 | MS.gene60402 | 0.87933 | 1.36E-69 | -1.69E-46 |
| MS.gene057146 | MS.gene60402 | 0.802759 | 4.82E-49 | -1.69E-46 |
| MS.gene057196 | MS.gene60402 | 0.808795 | 2.60E-50 | -1.69E-46 |
| MS.gene057331 | MS.gene60402 | 0.828457 | 8.90E-55 | -1.69E-46 |
| MS.gene057416 | MS.gene60402 | 0.836985 | 6.79E-57 | -1.69E-46 |
| MS.gene057926 | MS.gene60402 | 0.81104 | 8.54E-51 | -1.69E-46 |
| MS.gene058006 | MS.gene60402 | 0.806981 | 6.32E-50 | -1.69E-46 |
| MS.gene058019 | MS.gene60402 | 0.808559 | 2.92E-50 | -1.69E-46 |
| MS.gene058323 | MS.gene60402 | 0.833763 | 4.43E-56 | -1.69E-46 |
| MS.gene058419 | MS.gene60402 | 0.880147 | 6.97E-70 | -1.69E-46 |
| MS.gene058544 | MS.gene60402 | 0.825 | 5.95E-54 | -1.69E-46 |
| MS.gene058723 | MS.gene60402 | 0.851392 | 9.15E-61 | -1.69E-46 |
| MS.gene058773 | MS.gene60402 | 0.852053 | 5.94E-61 | -1.69E-46 |
| MS.gene05883 | MS.gene60402 | 0.803608 | 3.21E-49 | -1.69E-46 |
| MS.gene059022 | MS.gene60402 | 0.875629 | 2.65E-68 | -1.69E-46 |
| MS.gene059023 | MS.gene60402 | 0.820369 | 7.11E-53 | -1.69E-46 |
| MS.gene059029 | MS.gene60402 | 0.881779 | 1.81E-70 | -1.69E-46 |
| MS.gene05934 | MS.gene60402 | 0.824994 | 5.97E-54 | -1.69E-46 |
| MS.gene059536 | MS.gene60402 | 0.843149 | 1.67E-58 | -1.69E-46 |
| MS.gene059677 | MS.gene60402 | 0.889239 | 2.89E-73 | -1.69E-46 |
| MS.gene059718 | MS.gene60402 | 0.846881 | 1.64E-59 | -1.69E-46 |
| MS.gene059756 | MS.gene60402 | 0.827698 | 1.36E-54 | -1.69E-46 |
| MS.gene059858 | MS.gene60402 | 0.84122 | 5.42E-58 | -1.69E-46 |
| MS.gene059865 | MS.gene60402 | 0.810951 | 8.93E-51 | -1.69E-46 |
| MS.gene059902 | MS.gene60402 | 0.864017 | 1.65E-64 | -1.69E-46 |
| MS.gene059960 | MS.gene60402 | 0.867394 | 1.41E-65 | -1.69E-46 |
| MS.gene060193 | MS.gene60402 | 0.837245 | 5.82E-57 | -1.69E-46 |
| MS.gene060341 | MS.gene60402 | 0.815745 | 7.90E-52 | -1.69E-46 |
| MS.gene06046 | MS.gene60402 | 0.806093 | 9.73E-50 | -1.69E-46 |
| MS.gene060703 | MS.gene60402 | 0.832997 | 6.87E-56 | -1.69E-46 |
| MS.gene060727 | MS.gene60402 | 0.800902 | 1.16E-48 | -1.69E-46 |
| MS.gene060728 | MS.gene60402 | 0.84177 | 3.89E-58 | -1.69E-46 |
| MS.gene060762 | MS.gene60402 | 0.830251 | 3.26E-55 | -1.69E-46 |
| MS.gene06077 | MS.gene60402 | 0.831279 | 1.83E-55 | -1.69E-46 |
| MS.gene060804 | MS.gene60402 | 0.82773 | 1.33E-54 | -1.69E-46 |
| MS.gene060818 | MS.gene60402 | 0.815364 | 9.60E-52 | -1.69E-46 |
| MS.gene06092 | MS.gene60402 | 0.823809 | 1.13E-53 | -1.69E-46 |
| MS.gene061134 | MS.gene60402 | 0.80923 | 2.10E-50 | -1.69E-46 |
| MS.gene061150 | MS.gene60402 | 0.86684 | 2.12E-65 | -1.69E-46 |
| MS.gene061171 | MS.gene60402 | 0.803922 | 2.77E-49 | -1.69E-46 |
| MS.gene061200 | MS.gene60402 | 0.868122 | 8.25E-66 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60402.t1 | MTR_4g098500 | 91.406 | 128 | 11 | 0 | 1 | 128 | 1 | 128 | 4.98e-81 | 234 |
| MS.gene60402.t1 | MTR_4g098500 | 92.000 | 125 | 10 | 0 | 3 | 127 | 29 | 153 | 4.41e-79 | 230 |
| MS.gene60402.t1 | MTR_8g089310 | 74.545 | 110 | 28 | 0 | 5 | 114 | 2 | 111 | 3.77e-57 | 175 |
| MS.gene60402.t1 | MTR_8g089310 | 74.545 | 110 | 28 | 0 | 5 | 114 | 15 | 124 | 4.13e-57 | 176 |
| MS.gene60402.t1 | MTR_8g089310 | 74.545 | 110 | 28 | 0 | 5 | 114 | 49 | 158 | 5.57e-57 | 176 |
| MS.gene60402.t1 | MTR_2g047838 | 60.526 | 114 | 42 | 2 | 5 | 115 | 2 | 115 | 7.54e-47 | 149 |
| MS.gene60402.t1 | MTR_4g088850 | 54.545 | 88 | 35 | 2 | 44 | 128 | 40 | 125 | 1.11e-25 | 94.4 |
| MS.gene60402.t1 | MTR_4g088850 | 57.333 | 75 | 32 | 0 | 44 | 118 | 40 | 114 | 3.65e-24 | 90.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60402.t1 | AT1G32540 | 92.437 | 119 | 9 | 0 | 4 | 122 | 64 | 182 | 2.16e-76 | 224 |
| MS.gene60402.t1 | AT1G32540 | 93.220 | 118 | 8 | 0 | 5 | 122 | 32 | 149 | 2.45e-76 | 223 |
| MS.gene60402.t1 | AT1G32540 | 93.220 | 118 | 8 | 0 | 5 | 122 | 32 | 149 | 2.45e-76 | 223 |
| MS.gene60402.t1 | AT1G32540 | 99.074 | 108 | 1 | 0 | 5 | 112 | 32 | 139 | 1.88e-73 | 216 |
| MS.gene60402.t1 | AT1G32540 | 97.368 | 76 | 2 | 0 | 4 | 79 | 64 | 139 | 1.11e-48 | 153 |
| MS.gene60402.t1 | AT1G32540 | 100.000 | 74 | 0 | 0 | 5 | 78 | 32 | 105 | 3.50e-48 | 150 |
| MS.gene60402.t1 | AT1G32540 | 48.529 | 68 | 34 | 1 | 45 | 111 | 33 | 100 | 1.08e-11 | 57.8 |
| MS.gene60402.t1 | AT1G32540 | 100.000 | 74 | 0 | 0 | 5 | 78 | 32 | 105 | 3.50e-48 | 150 |
| MS.gene60402.t1 | AT1G32540 | 48.529 | 68 | 34 | 1 | 45 | 111 | 33 | 100 | 1.08e-11 | 57.8 |
| MS.gene60402.t1 | AT4G20380 | 61.157 | 121 | 36 | 3 | 5 | 114 | 7 | 127 | 3.73e-40 | 133 |
| MS.gene60402.t1 | AT4G20380 | 45.455 | 77 | 39 | 1 | 46 | 119 | 9 | 85 | 5.87e-15 | 68.2 |
| MS.gene60402.t1 | AT4G20380 | 61.157 | 121 | 36 | 3 | 5 | 114 | 7 | 127 | 3.73e-40 | 133 |
| MS.gene60402.t1 | AT4G20380 | 45.455 | 77 | 39 | 1 | 46 | 119 | 9 | 85 | 5.87e-15 | 68.2 |
| MS.gene60402.t1 | AT4G20380 | 60.656 | 122 | 37 | 3 | 5 | 115 | 2 | 123 | 3.75e-40 | 131 |
| MS.gene60402.t1 | AT4G20380 | 45.455 | 77 | 39 | 1 | 46 | 119 | 4 | 80 | 4.18e-15 | 67.4 |
| MS.gene60402.t1 | AT4G20380 | 61.157 | 121 | 36 | 3 | 5 | 114 | 2 | 122 | 4.89e-40 | 132 |
| MS.gene60402.t1 | AT4G20380 | 45.455 | 77 | 39 | 1 | 46 | 119 | 4 | 80 | 6.42e-15 | 68.2 |
| MS.gene60402.t1 | AT4G20380 | 61.157 | 121 | 36 | 3 | 5 | 114 | 2 | 122 | 4.89e-40 | 132 |
| MS.gene60402.t1 | AT4G20380 | 45.455 | 77 | 39 | 1 | 46 | 119 | 4 | 80 | 6.42e-15 | 68.2 |
| MS.gene60402.t1 | AT4G20380 | 61.157 | 121 | 36 | 3 | 5 | 114 | 2 | 122 | 4.89e-40 | 132 |
| MS.gene60402.t1 | AT4G20380 | 45.455 | 77 | 39 | 1 | 46 | 119 | 4 | 80 | 6.42e-15 | 68.2 |
| MS.gene60402.t1 | AT4G20380 | 61.157 | 121 | 36 | 3 | 5 | 114 | 2 | 122 | 4.89e-40 | 132 |
| MS.gene60402.t1 | AT4G20380 | 45.455 | 77 | 39 | 1 | 46 | 119 | 4 | 80 | 6.42e-15 | 68.2 |
| MS.gene60402.t1 | AT4G20380 | 61.157 | 121 | 36 | 3 | 5 | 114 | 2 | 122 | 4.89e-40 | 132 |
| MS.gene60402.t1 | AT4G20380 | 45.455 | 77 | 39 | 1 | 46 | 119 | 4 | 80 | 6.42e-15 | 68.2 |
| MS.gene60402.t1 | AT4G20380 | 51.020 | 147 | 35 | 3 | 5 | 114 | 2 | 148 | 7.56e-37 | 125 |
| MS.gene60402.t1 | AT4G20380 | 46.053 | 76 | 38 | 1 | 46 | 118 | 4 | 79 | 5.07e-15 | 68.9 |
| MS.gene60402.t1 | AT4G21610 | 46.903 | 113 | 58 | 1 | 18 | 130 | 34 | 144 | 1.08e-28 | 102 |
| MS.gene60402.t1 | AT4G21610 | 60.274 | 73 | 29 | 0 | 43 | 115 | 57 | 129 | 2.20e-26 | 96.7 |
Find 25 sgRNAs with CRISPR-Local
Find 70 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGGTCCAAGTACAGTAAA+AGG | 0.221457 | 8.1:+24405500 | MS.gene60402:CDS |
| CAGAGTCAACTTGTGTGTTC+TGG | 0.355331 | 8.1:+24404300 | MS.gene60402:CDS |
| ATGCTACTTATGTATCAATA+TGG | 0.369271 | 8.1:+24404843 | MS.gene60402:CDS |
| ATTGCAAACTGCACATTTCA+CGG | 0.413666 | 8.1:-24404874 | None:intergenic |
| GTGTATTGTGACAGCAAATC+AGG | 0.446436 | 8.1:+24404791 | MS.gene60402:intron |
| CATACAGAAGTGGCTCCAAC+TGG | 0.466065 | 8.1:-24404345 | None:intergenic |
| GGATGTACATGAGTAAAGTA+TGG | 0.469461 | 8.1:-24404567 | None:intergenic |
| GTCACAGCAGTGCCACCTCC+TGG | 0.473850 | 8.1:+24404387 | MS.gene60402:CDS |
| GAAATGGCTCAGTTAGTTTG+TGG | 0.475051 | 8.1:+24404538 | MS.gene60402:CDS |
| AAAAGGGTAAAGCAGTCAAA+TGG | 0.480658 | 8.1:+24405517 | MS.gene60402:CDS |
| TGTATTCACAGGCACAGAAA+TGG | 0.481941 | 8.1:+24404522 | MS.gene60402:intron |
| TCACACAGTCAATCTAGCTC+TGG | 0.518089 | 8.1:+24404621 | MS.gene60402:CDS |
| AACCTTCTTATGTATCCAGT+TGG | 0.523909 | 8.1:+24404330 | MS.gene60402:CDS |
| AAGGTCCAAGTACAGTAAAA+GGG | 0.533894 | 8.1:+24405501 | MS.gene60402:CDS |
| CTCCAACTGGATACATAAGA+AGG | 0.572621 | 8.1:-24404332 | None:intergenic |
| TATTTCATCATACCAGGAGG+TGG | 0.599736 | 8.1:-24404399 | None:intergenic |
| AACAGCACAGCATACAGAAG+TGG | 0.604154 | 8.1:-24404355 | None:intergenic |
| ATGGCTCAGTTAGTTTGTGG+AGG | 0.620989 | 8.1:+24404541 | MS.gene60402:CDS |
| ACAGTCAATCTAGCTCTGGA+AGG | 0.636792 | 8.1:+24404625 | MS.gene60402:CDS |
| ATGTCAACTGTGGTAACTGC+AGG | 0.637282 | 8.1:+24404820 | MS.gene60402:CDS |
| AACTATACTAACCCCAACTG+AGG | 0.647011 | 8.1:-24404904 | None:intergenic |
| ATTGAACACTCGTCGCTCCG+CGG | 0.692743 | 8.1:-24404588 | None:intergenic |
| ACTTTACTCATGTACATCCG+CGG | 0.709515 | 8.1:+24404571 | MS.gene60402:CDS |
| CAGGTGGCACATGTCAACTG+TGG | 0.714575 | 8.1:+24404810 | MS.gene60402:CDS |
| TATTGTGACAGCAAATCAGG+TGG | 0.775426 | 8.1:+24404794 | MS.gene60402:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAATAGTATATGATTTATT+AGG | - | chr8.1:24405116-24405135 | None:intergenic | 10.0% |
| !! | AAGTATGTGAAAGAAATAAA+TGG | - | chr8.1:24405461-24405480 | None:intergenic | 20.0% |
| !! | AATCATATACTATTATAGCA+AGG | + | chr8.1:24405120-24405139 | MS.gene60402:intron | 20.0% |
| !! | ATTCTTAAAACTACAAAGTA+CGG | - | chr8.1:24404766-24404785 | None:intergenic | 20.0% |
| !! | GTACTTAAATATCAATCTTA+AGG | + | chr8.1:24405173-24405192 | MS.gene60402:intron | 20.0% |
| !!! | TTATTTCTTTCACATACTTT+TGG | + | chr8.1:24405462-24405481 | MS.gene60402:intron | 20.0% |
| ! | AAAATAACTCTATACATGTG+AGG | - | chr8.1:24405233-24405252 | None:intergenic | 25.0% |
| ! | TATTTAAGTACCAAAAACCA+AGG | - | chr8.1:24405164-24405183 | None:intergenic | 25.0% |
| ! | TCAAAATAGAAAAGAGGAAA+AGG | - | chr8.1:24404945-24404964 | None:intergenic | 25.0% |
| ! | TTCTCATCAAAATAGAAAAG+AGG | - | chr8.1:24404951-24404970 | None:intergenic | 25.0% |
| !! | ATGCTACTTATGTATCAATA+TGG | + | chr8.1:24404843-24404862 | MS.gene60402:CDS | 25.0% |
| !!! | TTAAGCGTTTTTGTTTGAAT+TGG | + | chr8.1:24405276-24405295 | MS.gene60402:intron | 25.0% |
| !!! | TTCTACTTTTGTTCAATAAG+TGG | + | chr8.1:24405389-24405408 | MS.gene60402:intron | 25.0% |
| AGAGACCTAATAACTGATAA+GGG | - | chr8.1:24404431-24404450 | None:intergenic | 30.0% | |
| ATATCACAAAGTGAAGATTC+AGG | - | chr8.1:24404485-24404504 | None:intergenic | 30.0% | |
| ATTTACCTTACCAAAAGATG+TGG | - | chr8.1:24405363-24405382 | None:intergenic | 30.0% | |
| GAAGAATATTTCATCATACC+AGG | - | chr8.1:24404408-24404427 | None:intergenic | 30.0% | |
| GTGAATACATCAGGTAAATA+TGG | - | chr8.1:24404511-24404530 | None:intergenic | 30.0% | |
| TAGAGACCTAATAACTGATA+AGG | - | chr8.1:24404432-24404451 | None:intergenic | 30.0% | |
| TATTTACCTGATGTATTCAC+AGG | + | chr8.1:24404511-24404530 | MS.gene60402:intron | 30.0% | |
| TGAAAGAAATAAATGGTGCT+TGG | - | chr8.1:24405454-24405473 | None:intergenic | 30.0% | |
| ! | AAAAACCACATCTTTTGGTA+AGG | + | chr8.1:24405355-24405374 | MS.gene60402:intron | 30.0% |
| ! | TATGCAAAAACCACATCTTT+TGG | + | chr8.1:24405350-24405369 | MS.gene60402:intron | 30.0% |
| !!! | AAACTATGTCTTGTTTTGCA+AGG | - | chr8.1:24404995-24405014 | None:intergenic | 30.0% |
| AAAAGGGTAAAGCAGTCAAA+TGG | + | chr8.1:24405517-24405536 | MS.gene60402:CDS | 35.0% | |
| AAAGGTCCAAGTACAGTAAA+AGG | + | chr8.1:24405500-24405519 | MS.gene60402:CDS | 35.0% | |
| AACCTTCTTATGTATCCAGT+TGG | + | chr8.1:24404330-24404349 | MS.gene60402:CDS | 35.0% | |
| AAGGTCCAAGTACAGTAAAA+GGG | + | chr8.1:24405501-24405520 | MS.gene60402:CDS | 35.0% | |
| ATTGCAAACTGCACATTTCA+CGG | - | chr8.1:24404877-24404896 | None:intergenic | 35.0% | |
| CGAGTTAAATTGCTGTGATT+CGG | + | chr8.1:24404737-24404756 | MS.gene60402:intron | 35.0% | |
| GAAAAGAGGAAAAGGTAGTA+TGG | - | chr8.1:24404937-24404956 | None:intergenic | 35.0% | |
| GAATATTTCATCATACCAGG+AGG | - | chr8.1:24404405-24404424 | None:intergenic | 35.0% | |
| GAGACCTAATAACTGATAAG+GGG | - | chr8.1:24404430-24404449 | None:intergenic | 35.0% | |
| GGATGTACATGAGTAAAGTA+TGG | - | chr8.1:24404570-24404589 | None:intergenic | 35.0% | |
| TCTTCCCCTTATCAGTTATT+AGG | + | chr8.1:24404423-24404442 | MS.gene60402:intron | 35.0% | |
| ! | ATGTTACTGAATGATGATGC+AGG | + | chr8.1:24405054-24405073 | MS.gene60402:intron | 35.0% |
| ! | CAATTTTGTTACCTCAGTTG+GGG | + | chr8.1:24404893-24404912 | MS.gene60402:CDS | 35.0% |
| ! | CTTTACCCTTTTACTGTACT+TGG | - | chr8.1:24405509-24405528 | None:intergenic | 35.0% |
| ! | GCAATTTTGTTACCTCAGTT+GGG | + | chr8.1:24404892-24404911 | MS.gene60402:CDS | 35.0% |
| ! | GGACCTTTTCTATTTCCAAA+AGG | - | chr8.1:24405488-24405507 | None:intergenic | 35.0% |
| ! | TGCAATTTTGTTACCTCAGT+TGG | + | chr8.1:24404891-24404910 | MS.gene60402:CDS | 35.0% |
| !! | CTGCTATATTTTTGTGTGTG+TGG | + | chr8.1:24405424-24405443 | MS.gene60402:intron | 35.0% |
| !! | TGGCCTTTTGGAAATAGAAA+AGG | + | chr8.1:24405482-24405501 | MS.gene60402:intron | 35.0% |
| !! | TTCACATACTTTTGGCCTTT+TGG | + | chr8.1:24405470-24405489 | MS.gene60402:intron | 35.0% |
| AACTATACTAACCCCAACTG+AGG | - | chr8.1:24404907-24404926 | None:intergenic | 40.0% | |
| ACTTTACTCATGTACATCCG+CGG | + | chr8.1:24404571-24404590 | MS.gene60402:CDS | 40.0% | |
| CATTACAGACTCTACAACCT+TGG | + | chr8.1:24405144-24405163 | MS.gene60402:intron | 40.0% | |
| CTCCAACTGGATACATAAGA+AGG | - | chr8.1:24404335-24404354 | None:intergenic | 40.0% | |
| GTGTATTGTGACAGCAAATC+AGG | + | chr8.1:24404791-24404810 | MS.gene60402:intron | 40.0% | |
| TATTGTGACAGCAAATCAGG+TGG | + | chr8.1:24404794-24404813 | MS.gene60402:intron | 40.0% | |
| TATTTCATCATACCAGGAGG+TGG | - | chr8.1:24404402-24404421 | None:intergenic | 40.0% | |
| TGTATTCACAGGCACAGAAA+TGG | + | chr8.1:24404522-24404541 | MS.gene60402:intron | 40.0% | |
| ! | GAAATGGCTCAGTTAGTTTG+TGG | + | chr8.1:24404538-24404557 | MS.gene60402:CDS | 40.0% |
| !! | GACTCTACAACCTTGGTTTT+TGG | + | chr8.1:24405151-24405170 | MS.gene60402:intron | 40.0% |
| !!! | GTTATTTTTCCCTTGCGTGA+CGG | + | chr8.1:24405245-24405264 | MS.gene60402:intron | 40.0% |
| AACAGCACAGCATACAGAAG+TGG | - | chr8.1:24404358-24404377 | None:intergenic | 45.0% | |
| AATACAATCACCGTCACGCA+AGG | - | chr8.1:24405258-24405277 | None:intergenic | 45.0% | |
| ATACAATCACCGTCACGCAA+GGG | - | chr8.1:24405257-24405276 | None:intergenic | 45.0% | |
| ATGTCAACTGTGGTAACTGC+AGG | + | chr8.1:24404820-24404839 | MS.gene60402:CDS | 45.0% | |
| CAGAGTCAACTTGTGTGTTC+TGG | + | chr8.1:24404300-24404319 | MS.gene60402:CDS | 45.0% | |
| TCTGTGCCTGTGAATACATC+AGG | - | chr8.1:24404520-24404539 | None:intergenic | 45.0% | |
| ! | TCACACAGTCAATCTAGCTC+TGG | + | chr8.1:24404621-24404640 | MS.gene60402:CDS | 45.0% |
| !! | ACAGTCAATCTAGCTCTGGA+AGG | + | chr8.1:24404625-24404644 | MS.gene60402:CDS | 45.0% |
| !! | ATGGCTCAGTTAGTTTGTGG+AGG | + | chr8.1:24404541-24404560 | MS.gene60402:CDS | 45.0% |
| CATACAGAAGTGGCTCCAAC+TGG | - | chr8.1:24404348-24404367 | None:intergenic | 50.0% | |
| ATTGAACACTCGTCGCTCCG+CGG | - | chr8.1:24404591-24404610 | None:intergenic | 55.0% | |
| CAGGTGGCACATGTCAACTG+TGG | + | chr8.1:24404810-24404829 | MS.gene60402:CDS | 55.0% | |
| GCAGGCCTCAGCAAGCACGA+CGG | + | chr8.1:24405072-24405091 | MS.gene60402:intron | 65.0% | |
| GTCACAGCAGTGCCACCTCC+TGG | + | chr8.1:24404387-24404406 | MS.gene60402:CDS | 65.0% | |
| ! | CTGCTCCGTCGTGCTTGCTG+AGG | - | chr8.1:24405080-24405099 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 24404288 | 24405552 | 24404288 | ID=MS.gene60402 |
| chr8.1 | mRNA | 24404288 | 24405552 | 24404288 | ID=MS.gene60402.t1;Parent=MS.gene60402 |
| chr8.1 | exon | 24404288 | 24404408 | 24404288 | ID=MS.gene60402.t1.exon1;Parent=MS.gene60402.t1 |
| chr8.1 | CDS | 24404288 | 24404408 | 24404288 | ID=cds.MS.gene60402.t1;Parent=MS.gene60402.t1 |
| chr8.1 | exon | 24404533 | 24404646 | 24404533 | ID=MS.gene60402.t1.exon2;Parent=MS.gene60402.t1 |
| chr8.1 | CDS | 24404533 | 24404646 | 24404533 | ID=cds.MS.gene60402.t1;Parent=MS.gene60402.t1 |
| chr8.1 | exon | 24404805 | 24404914 | 24404805 | ID=MS.gene60402.t1.exon3;Parent=MS.gene60402.t1 |
| chr8.1 | CDS | 24404805 | 24404914 | 24404805 | ID=cds.MS.gene60402.t1;Parent=MS.gene60402.t1 |
| chr8.1 | exon | 24405499 | 24405552 | 24405499 | ID=MS.gene60402.t1.exon4;Parent=MS.gene60402.t1 |
| chr8.1 | CDS | 24405499 | 24405552 | 24405499 | ID=cds.MS.gene60402.t1;Parent=MS.gene60402.t1 |
| Gene Sequence |
| Protein sequence |
