AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene70534


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene70534.t1 MTR_1g021965 61.538 52 20 0 18 69 112 163 2.90e-15 71.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 45 sgRNAs with CRISPR-Local

Find 89 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATGCCAACGACGGAAATTA+TGG 0.246928 3.1:-67479388 MS.gene70534:CDS
TTCCACAATCTAAGGAATTA+TGG 0.275297 3.1:+67479229 None:intergenic
TTCTCTCGACTCCAAAGCTT+TGG 0.344968 3.1:+67480288 None:intergenic
CACCTGCCTTCCACAATCTA+AGG 0.356067 3.1:+67479221 None:intergenic
GTTGTTTGCGTGTATTGTTT+TGG 0.356104 3.1:+67480257 None:intergenic
TGCTCTCGAGTGCAAAGCTT+TGG 0.369375 3.1:-67479894 MS.gene70534:CDS
AGCTGCACGAGCCAAAGCTT+TGG 0.379774 3.1:-67480299 MS.gene70534:CDS
GCGGAGAACATCATACTTCC+AGG 0.389492 3.1:-67480469 MS.gene70534:CDS
ACTTCAATTCTGGAAACGTC+TGG 0.392283 3.1:-67479356 MS.gene70534:CDS
TCTCCGCCTTCAATTCTTCA+AGG 0.400192 3.1:+67480485 None:intergenic
CGTGTTTGTCGCTGATGCCT+TGG 0.446247 3.1:-67480446 MS.gene70534:CDS
CCTTCAATTCTTCAAGGAGT+TGG 0.448637 3.1:+67480491 None:intergenic
CCAACTCCTTGAAGAATTGA+AGG 0.465521 3.1:-67480491 MS.gene70534:CDS
TTCCTTAGATTGTGGAAGGC+AGG 0.477616 3.1:-67479223 MS.gene70534:CDS
TTGTAATCATTCCATGAGTC+GGG 0.479069 3.1:+67480412 None:intergenic
TGTCTCTTAATGATCTCATC+AGG 0.486426 3.1:+67479729 None:intergenic
AAGGCCATAATTTCCGTCGT+TGG 0.491561 3.1:+67479384 None:intergenic
TTGAAAAGTTGCCCGACTCA+TGG 0.500599 3.1:-67480423 MS.gene70534:CDS
AGCTCCGCATTCCATTCTGC+TGG 0.500843 3.1:-67479771 MS.gene70534:CDS
AGCTCCGCATTCCATTCTGC+TGG 0.500843 3.1:-67479864 MS.gene70534:CDS
CATTGAAGACACCAGCAGAA+TGG 0.516038 3.1:+67479760 None:intergenic
ACAATCTAAGGAATTATGGC+TGG 0.522622 3.1:+67479233 None:intergenic
AGCCATAATTCCTTAGATTG+TGG 0.526215 3.1:-67479231 MS.gene70534:CDS
CATTGAAGACACCAGCAGAA+TGG 0.535892 3.1:+67479853 None:intergenic
CTTGTAATCATTCCATGAGT+CGG 0.537899 3.1:+67480411 None:intergenic
CTTCAATTCTGGAAACGTCT+GGG 0.550347 3.1:-67479355 MS.gene70534:CDS
TCTGTAATGGCAAATGAAAG+AGG 0.555816 3.1:-67480548 None:intergenic
TACTGATCCTCCTCCGACAG+TGG 0.555859 3.1:+67479313 None:intergenic
GCATCAGCGACAAACACGCC+TGG 0.558250 3.1:+67480451 None:intergenic
ATAATTCCTTAGATTGTGGA+AGG 0.574451 3.1:-67479227 MS.gene70534:CDS
TAGGTGACCACTGTCGGAGG+AGG 0.579910 3.1:-67479320 MS.gene70534:CDS
TTTGCATAGGTGACCACTGT+CGG 0.584337 3.1:-67479326 MS.gene70534:CDS
TGTGGAAGGCAGGTGACTGA+AGG 0.588000 3.1:-67479213 MS.gene70534:CDS
GAGGATCAGTAGCAACAACA+AGG 0.606584 3.1:-67479301 MS.gene70534:CDS
TGACAATAAGAAGAATGTCT+CGG 0.627026 3.1:+67479271 None:intergenic
ATGGCAAATGAAAGAGGACA+AGG 0.655713 3.1:-67480542 None:intergenic
AGATGCGTTCATGCCAACGA+CGG 0.660184 3.1:-67479397 MS.gene70534:CDS
GCATAGGTGACCACTGTCGG+AGG 0.668853 3.1:-67479323 MS.gene70534:CDS
ACAATACACGCAAACAACAG+AGG 0.686335 3.1:-67480252 MS.gene70534:intron
AGGATCAGTAGCAACAACAA+GGG 0.691715 3.1:-67479300 MS.gene70534:CDS
GACACCAGCAGAAAAGAATG+CGG 0.696693 3.1:-67480322 MS.gene70534:CDS
GACACCAGCAGAATGGAATG+CGG 0.699910 3.1:+67479767 None:intergenic
GACACCAGCAGAATGGAATG+CGG 0.699910 3.1:+67479860 None:intergenic
AGGACAAGGAAATCAAAGTG+CGG 0.706319 3.1:-67480528 MS.gene70534:CDS
ACTCCTTGAAGAATTGAAGG+CGG 0.711654 3.1:-67480488 MS.gene70534:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TATAGATATCGAAAAGTGAA+TGG + chr3.1:67479673-67479692 None:intergenic 25.0%
!! ACCTAGGTTTGATTTAAAAT+TGG - chr3.1:67479733-67479752 MS.gene70534:CDS 25.0%
!! CCTAGGTTTGATTTAAAATT+GGG - chr3.1:67479734-67479753 MS.gene70534:CDS 25.0%
!! CTAGGTTTGATTTAAAATTG+GGG - chr3.1:67479735-67479754 MS.gene70534:CDS 25.0%
AATAAAACTGATCATACCCT+CGG + chr3.1:67479770-67479789 None:intergenic 30.0%
AATTATTGCTTGTATGAGTC+AGG - chr3.1:67480040-67480059 MS.gene70534:intron 30.0%
ATAATTCCTTAGATTGTGGA+AGG - chr3.1:67480499-67480518 MS.gene70534:CDS 30.0%
TACGTTATCTGATTTACGTT+TGG + chr3.1:67480266-67480285 None:intergenic 30.0%
TGACAATAAGAAGAATGTCT+CGG + chr3.1:67480458-67480477 None:intergenic 30.0%
TTCCACAATCTAAGGAATTA+TGG + chr3.1:67480500-67480519 None:intergenic 30.0%
! ATAACGTAGAAAGAAGTGTA+TGG - chr3.1:67480278-67480297 MS.gene70534:CDS 30.0%
! CCCAATTTTAAATCAAACCT+AGG + chr3.1:67479737-67479756 None:intergenic 30.0%
! CTAGGTTCGATTTAAAATTG+AGG + chr3.1:67479538-67479557 None:intergenic 30.0%
! TCCAGAATTGAAGTTTTTGA+AGG + chr3.1:67480364-67480383 None:intergenic 30.0%
!! ACAACATGAAAGTTTGTTGT+TGG + chr3.1:67480204-67480223 None:intergenic 30.0%
AATTTCTTTGGCAACCTTGT+AGG - chr3.1:67480228-67480247 MS.gene70534:intron 35.0%
ACAAGGTTGCCAAAGAAATT+TGG + chr3.1:67480228-67480247 None:intergenic 35.0%
ACAATCTAAGGAATTATGGC+TGG + chr3.1:67480496-67480515 None:intergenic 35.0%
AGCCATAATTCCTTAGATTG+TGG - chr3.1:67480495-67480514 MS.gene70534:CDS 35.0%
CAAGGTTGCCAAAGAAATTT+GGG + chr3.1:67480227-67480246 None:intergenic 35.0%
CATGTTGTCCCAAATTTCTT+TGG - chr3.1:67480216-67480235 MS.gene70534:intron 35.0%
CCTTCAATTCTTAAAGGAGT+TGG - chr3.1:67480119-67480138 MS.gene70534:intron 35.0%
CTTGTAATCATTCCATGAGT+CGG + chr3.1:67479318-67479337 None:intergenic 35.0%
GCCTTCAAAAACTTCAATTC+TGG - chr3.1:67480360-67480379 MS.gene70534:CDS 35.0%
GTTGTTGTGTGTGTAAAGAA+AGG + chr3.1:67479712-67479731 None:intergenic 35.0%
TCTGTGATGACAAATGAAAG+AGG + chr3.1:67480175-67480194 None:intergenic 35.0%
TGTCTCTTAATGATCTCATC+AGG + chr3.1:67480000-67480019 None:intergenic 35.0%
TTGTAATCATTCCATGAGTC+GGG + chr3.1:67479317-67479336 None:intergenic 35.0%
! AATTGAGGTTAGTTACTGTG+AGG + chr3.1:67479523-67479542 None:intergenic 35.0%
! ACTCCTTTAAGAATTGAAGG+CGG + chr3.1:67480119-67480138 None:intergenic 35.0%
! ATTGAGGTTAGTTACTGTGA+GGG + chr3.1:67479522-67479541 None:intergenic 35.0%
! CCAACTCCTTTAAGAATTGA+AGG + chr3.1:67480122-67480141 None:intergenic 35.0%
! GTTGTTTGCGTGTATTGTTT+TGG - chr3.1:67479801-67479820 MS.gene70534:intron 35.0%
! GTTGTTTGCGTGTATTGTTT+TGG + chr3.1:67479820-67479801 None:intergenic 35.0%
!! ACCTGAGTCAAATTTGCTTT+TGG - chr3.1:67479634-67479653 MS.gene70534:intron 35.0%
!!! AAATTTGCTTTTGGCTTAGG+AGG - chr3.1:67479643-67479662 MS.gene70534:intron 35.0%
!!! GTCAAATTTGCTTTTGGCTT+AGG - chr3.1:67479640-67479659 MS.gene70534:intron 35.0%
AAGCATATCGCTGCAGATAT+TGG - chr3.1:67479591-67479610 MS.gene70534:intron 40.0%
ACAATACACGCAAACAACAG+AGG - chr3.1:67479474-67479493 MS.gene70534:intron 40.0%
ACAATACACGCAAACAACAG+AGG + chr3.1:67479493-67479474 None:intergenic 40.0%
ACTCCTTGAAGAATTGAAGG+CGG - chr3.1:67479238-67479257 MS.gene70534:CDS 40.0%
AGGACAAGGAAATCAAAGTG+CGG - chr3.1:67479198-67479217 MS.gene70534:CDS 40.0%
AGGATCAGTAGCAACAACAA+GGG - chr3.1:67480426-67480445 MS.gene70534:CDS 40.0%
CACACACATAGCAACAATCT+AGG + chr3.1:67479556-67479575 None:intergenic 40.0%
CCAACTCCTTGAAGAATTGA+AGG - chr3.1:67479235-67479254 MS.gene70534:CDS 40.0%
CCTTCAATTCTTCAAGGAGT+TGG + chr3.1:67479238-67479257 None:intergenic 40.0%
CTAGATTGTTGCTATGTGTG+TGG - chr3.1:67479554-67479573 MS.gene70534:intron 40.0%
GCCAAAAGCAAATTTGACTC+AGG + chr3.1:67479638-67479657 None:intergenic 40.0%
GGATGTTTACTGTTCCTACA+AGG + chr3.1:67480245-67480264 None:intergenic 40.0%
TACACACACAACAACAACCT+AGG - chr3.1:67479717-67479736 MS.gene70534:CDS 40.0%
TCTCCGCCTTCAATTCTTAA+AGG - chr3.1:67480113-67480132 MS.gene70534:intron 40.0%
TGCTATGTGTGTGGAAAGAA+AGG - chr3.1:67479563-67479582 MS.gene70534:intron 40.0%
! ACTTCAATTCTGGAAACGTC+TGG - chr3.1:67480370-67480389 MS.gene70534:CDS 40.0%
! CTTCAATTCTGGAAACGTCT+GGG - chr3.1:67480371-67480390 MS.gene70534:CDS 40.0%
AAGGCCATAATTTCCGTCGT+TGG + chr3.1:67480345-67480364 None:intergenic 45.0%
CATGCCAACGACGGAAATTA+TGG - chr3.1:67480338-67480357 MS.gene70534:CDS 45.0%
CATTGAAGACACCAGCAGAA+TGG + chr3.1:67479416-67479397 None:intergenic 45.0%
GACACCAGCAGAAAAGAATG+CGG - chr3.1:67479404-67479423 MS.gene70534:CDS 45.0%
GAGGATCAGTAGCAACAACA+AGG - chr3.1:67480425-67480444 MS.gene70534:CDS 45.0%
TCTCCGCCTTCAATTCTTCA+AGG + chr3.1:67479244-67479263 None:intergenic 45.0%
TTCCTTAGATTGTGGAAGGC+AGG - chr3.1:67480503-67480522 MS.gene70534:CDS 45.0%
TTCTCTCGACTCCAAAGCTT+TGG + chr3.1:67479441-67479460 None:intergenic 45.0%
TTGAAAAGTTGCCCGACTCA+TGG - chr3.1:67479303-67479322 MS.gene70534:CDS 45.0%
TTTGCATAGGTGACCACTGT+CGG - chr3.1:67480400-67480419 MS.gene70534:CDS 45.0%
! GTCAGGCAACTTTTCAACCA+AGG - chr3.1:67480057-67480076 MS.gene70534:intron 45.0%
! GTCTGGGAACTGTTTTGCAT+AGG - chr3.1:67480387-67480406 MS.gene70534:CDS 45.0%
! TTGCTCTGAGTGCAAAGCTT+TGG - chr3.1:67479925-67479944 MS.gene70534:intron 45.0%
!! AATTGGGGTTAGTTACTCCG+AGG - chr3.1:67479750-67479769 MS.gene70534:CDS 45.0%
!! ATTGGGGTTAGTTACTCCGA+GGG - chr3.1:67479751-67479770 MS.gene70534:CDS 45.0%
!!! TTGCTTTTGGCTTAGGAGGT+TGG - chr3.1:67479647-67479666 MS.gene70534:intron 45.0%
AGATGCGTTCATGCCAACGA+CGG - chr3.1:67480329-67480348 MS.gene70534:CDS 50.0%
CACCTGCCTTCCACAATCTA+AGG + chr3.1:67480508-67480527 None:intergenic 50.0%
CTTCACACTGCTAGACATCC+AGG + chr3.1:67480311-67480330 None:intergenic 50.0%
CTTTCACACGTGTTTGTCGC+TGG + chr3.1:67480085-67480104 None:intergenic 50.0%
GAAGTGTATGGCGACAAACC+TGG - chr3.1:67480290-67480309 MS.gene70534:CDS 50.0%
GACACCAGCAGAATGGAATG+CGG + chr3.1:67479869-67479888 None:intergenic 50.0%
GCGGAGAACATCATACTTCC+AGG - chr3.1:67479257-67479276 MS.gene70534:CDS 50.0%
TGCTCTCGAGTGCAAAGCTT+TGG - chr3.1:67479832-67479851 MS.gene70534:intron 50.0%
! AGCTCCGCATTCTTTTCTGC+TGG + chr3.1:67479411-67479430 None:intergenic 50.0%
! GTCGGGCAACTTTTCAACCA+AGG + chr3.1:67479300-67479319 None:intergenic 50.0%
AGCTCCGCATTCCATTCTGC+TGG - chr3.1:67479862-67479881 MS.gene70534:CDS 55.0%
AGCTGCACGAGCCAAAGCTT+TGG - chr3.1:67479427-67479446 MS.gene70534:intron 55.0%
CGTGTTTGTCGCTGATGCCT+TGG - chr3.1:67479280-67479299 MS.gene70534:CDS 55.0%
TACTGATCCTCCTCCGACAG+TGG + chr3.1:67480416-67480435 None:intergenic 55.0%
TGTGGAAGGCAGGTGACTGA+AGG - chr3.1:67480513-67480532 MS.gene70534:CDS 55.0%
CGTGTTTGTCGCTGGTGCCT+TGG + chr3.1:67480077-67480096 None:intergenic 60.0%
GCATAGGTGACCACTGTCGG+AGG - chr3.1:67480403-67480422 MS.gene70534:CDS 60.0%
GCATCAGCGACAAACACGCC+TGG + chr3.1:67479278-67479297 None:intergenic 60.0%
TAGGTGACCACTGTCGGAGG+AGG - chr3.1:67480406-67480425 MS.gene70534:CDS 60.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 67479191 67480557 67479191 ID=MS.gene70534
chr3.1 mRNA 67479191 67480557 67479191 ID=MS.gene70534.t1;Parent=MS.gene70534
chr3.1 exon 67480253 67480557 67480253 ID=MS.gene70534.t1.exon1;Parent=MS.gene70534.t1
chr3.1 CDS 67480253 67480557 67480253 ID=cds.MS.gene70534.t1;Parent=MS.gene70534.t1
chr3.1 exon 67479851 67479919 67479851 ID=MS.gene70534.t1.exon2;Parent=MS.gene70534.t1
chr3.1 CDS 67479851 67479919 67479851 ID=cds.MS.gene70534.t1;Parent=MS.gene70534.t1
chr3.1 exon 67479698 67479791 67479698 ID=MS.gene70534.t1.exon3;Parent=MS.gene70534.t1
chr3.1 CDS 67479698 67479791 67479698 ID=cds.MS.gene70534.t1;Parent=MS.gene70534.t1
chr3.1 exon 67479191 67479424 67479191 ID=MS.gene70534.t1.exon4;Parent=MS.gene70534.t1
chr3.1 CDS 67479191 67479424 67479191 ID=cds.MS.gene70534.t1;Parent=MS.gene70534.t1
Gene Sequence

>MS.gene70534

ATGAAAGAGGACAAGGAAATCAAAGTGCGGATCAATGAGTATCACCAACTCCTTGAAGAATTGAAGGCGGAGAACATCATACTTCCAGGCGTGTTTGTCGCTGATGCCTTGGTTGAAAAGTTGCCCGACTCATGGAATGATTACAAGCAACAGCTGAAACATAAGCACAAGCAGATGTTTCTTAATGATCTCATCAAACACATAATCATTGAAGACACCAGCAGAAAAGAATGCGGAGCTGCACGAGCCAAAGCTTTGGAGTCGAGAGAAAATCTAATCCAAAACAATACACGCAAACAACAGAGGTATGAAAATAAAAATGATTATGCCCTCACAGTAACTAACCTCAATTTTAAATCGAACCTAGATTGTTGCTATGTGTGTGGAAAGAAAGGTCATAAAGCATATCGCTGCAGATATTGGAAAGTGAATCGAACATTCGAACCTGAGTCAAATTTGCTTTTGGCTTAGGAGGTTGGCCATTCACTTTTCGATATCTATAGTGACATGCTTTATGACCTTTCTTTACACACACAACAACAACCTAGGTTTGATTTAAAATTGGGGTTAGTTACTCCGAGGGTATGATCAGTTTTATTTTCATACCTCTGTTGTTTGCGTGTATTGTTTTGGATTAGATTTGCTCTCGAGTGCAAAGCTTTGGCTCGTGCAGCTCCGCATTCCATTCTGCTGGTGTCTTCAATGTTGTTTGCATGTATTGTTTTTGATTAGATTTGCTCTGAGTGCAAAGCTTTGGCTCGTGCAGCTCCGCATTCCATTCTGCTGGTGTCTTCAATGATTATGTGCCTGATGAGATCATTAAGAGACATCTGTTTGTGCTTATGTTTCAATTATTGCTTGTATGAGTCAGGCAACTTTTCAACCAAGGCACCAGCGACAAACACGTGTGAAAGTATGATGTTCTCCGCCTTCAATTCTTAAAGGAGTTGGTAGTGATAATCTGCACTTTGATTTCTTTGTCCTCTTTCATTTGTCATCACAGATAGTTTCCAACAACAAACTTTCATGTTGTCCCAAATTTCTTTGGCAACCTTGTAGGAACAGTAAACATCCAAACGTAAATCAGATAACGTAGAAAGAAGTGTATGGCGACAAACCTGGATGTCTAGCAGTGTGAAGATGCGTTCATGCCAACGACGGAAATTATGGCCTTCAAAAACTTCAATTCTGGAAACGTCTGGGAACTGTTTTGCATAGGTGACCACTGTCGGAGGAGGATCAGTAGCAACAACAAGGGTAGAGTCCGAGACATTCTTCTTATTGTCATTGTTAGAATCATCTCCAGCCATAATTCCTTAGATTGTGGAAGGCAGGTGACTGAAGGATCACAACCTGCAAAACAATAA

Protein sequence

>MS.gene70534.t1

MKEDKEIKVRINEYHQLLEELKAENIILPGVFVADALVEKLPDSWNDYKQQLKHKHKQMFLNDLIKHIIIEDTSRKECGAARAKALESRENLIQNNTRKQQRFALECKALARAAPHSILLVSSMFSAFHSAGVFNDYVPDEIIKRHLFVLMFQLLLCEDAFMPTTEIMAFKNFNSGNVWELFCIGDHCRRRISSNNKGRVRDILLIVIVRIISSHNSLDCGRQVTEGSQPAKQ