Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88520.t1 | XP_013468793.1 | 96.2 | 133 | 3 | 1 | 3 | 135 | 2 | 132 | 4.70E-66 | 260.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88520.t1 | A0A072VXN1 | 96.2 | 133 | 3 | 1 | 3 | 135 | 2 | 132 | 3.4e-66 | 260.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049351 | MS.gene88520 | 0.845339 | 4.32E-59 | -1.69E-46 |
| MS.gene049356 | MS.gene88520 | 0.830647 | 2.61E-55 | -1.69E-46 |
| MS.gene049369 | MS.gene88520 | 0.824079 | 9.80E-54 | -1.69E-46 |
| MS.gene049427 | MS.gene88520 | 0.824294 | 8.73E-54 | -1.69E-46 |
| MS.gene049609 | MS.gene88520 | 0.834413 | 3.04E-56 | -1.69E-46 |
| MS.gene049661 | MS.gene88520 | 0.837495 | 5.02E-57 | -1.69E-46 |
| MS.gene049662 | MS.gene88520 | 0.826812 | 2.21E-54 | -1.69E-46 |
| MS.gene049805 | MS.gene88520 | 0.824611 | 7.35E-54 | -1.69E-46 |
| MS.gene049907 | MS.gene88520 | 0.81361 | 2.35E-51 | -1.69E-46 |
| MS.gene050076 | MS.gene88520 | 0.821863 | 3.22E-53 | -1.69E-46 |
| MS.gene050301 | MS.gene88520 | 0.825531 | 4.45E-54 | -1.69E-46 |
| MS.gene050397 | MS.gene88520 | 0.82921 | 5.85E-55 | -1.69E-46 |
| MS.gene050432 | MS.gene88520 | 0.863217 | 2.91E-64 | -1.69E-46 |
| MS.gene050520 | MS.gene88520 | 0.825788 | 3.87E-54 | -1.69E-46 |
| MS.gene050593 | MS.gene88520 | 0.834653 | 2.65E-56 | -1.69E-46 |
| MS.gene050670 | MS.gene88520 | 0.842401 | 2.64E-58 | -1.69E-46 |
| MS.gene050767 | MS.gene88520 | 0.826158 | 3.16E-54 | -1.69E-46 |
| MS.gene050770 | MS.gene88520 | 0.827303 | 1.69E-54 | -1.69E-46 |
| MS.gene050772 | MS.gene88520 | 0.834394 | 3.07E-56 | -1.69E-46 |
| MS.gene050783 | MS.gene88520 | 0.80042 | 1.45E-48 | -1.69E-46 |
| MS.gene05101 | MS.gene88520 | 0.83259 | 8.67E-56 | -1.69E-46 |
| MS.gene05105 | MS.gene88520 | 0.869114 | 3.95E-66 | -1.69E-46 |
| MS.gene051255 | MS.gene88520 | 0.852911 | 3.38E-61 | -1.69E-46 |
| MS.gene051360 | MS.gene88520 | 0.810186 | 1.31E-50 | -1.69E-46 |
| MS.gene051387 | MS.gene88520 | 0.849299 | 3.53E-60 | -1.69E-46 |
| MS.gene051666 | MS.gene88520 | 0.803469 | 3.43E-49 | -1.69E-46 |
| MS.gene051692 | MS.gene88520 | 0.842823 | 2.04E-58 | -1.69E-46 |
| MS.gene051810 | MS.gene88520 | 0.814408 | 1.56E-51 | -1.69E-46 |
| MS.gene052078 | MS.gene88520 | 0.819675 | 1.03E-52 | -1.69E-46 |
| MS.gene052188 | MS.gene88520 | 0.830844 | 2.34E-55 | -1.69E-46 |
| MS.gene052253 | MS.gene88520 | 0.822351 | 2.48E-53 | -1.69E-46 |
| MS.gene052271 | MS.gene88520 | 0.812991 | 3.21E-51 | -1.69E-46 |
| MS.gene052310 | MS.gene88520 | 0.829367 | 5.36E-55 | -1.69E-46 |
| MS.gene052322 | MS.gene88520 | 0.859762 | 3.30E-63 | -1.69E-46 |
| MS.gene052340 | MS.gene88520 | 0.816596 | 5.10E-52 | -1.69E-46 |
| MS.gene052421 | MS.gene88520 | 0.815702 | 8.08E-52 | -1.69E-46 |
| MS.gene052481 | MS.gene88520 | 0.840421 | 8.78E-58 | -1.69E-46 |
| MS.gene052484 | MS.gene88520 | 0.858245 | 9.41E-63 | -1.69E-46 |
| MS.gene052485 | MS.gene88520 | 0.84929 | 3.56E-60 | -1.69E-46 |
| MS.gene052508 | MS.gene88520 | 0.804791 | 1.82E-49 | -1.69E-46 |
| MS.gene052615 | MS.gene88520 | 0.841494 | 4.59E-58 | -1.69E-46 |
| MS.gene052982 | MS.gene88520 | 0.830788 | 2.41E-55 | -1.69E-46 |
| MS.gene053104 | MS.gene88520 | 0.814094 | 1.83E-51 | -1.69E-46 |
| MS.gene053107 | MS.gene88520 | 0.810299 | 1.24E-50 | -1.69E-46 |
| MS.gene053207 | MS.gene88520 | 0.868264 | 7.43E-66 | -1.69E-46 |
| MS.gene053209 | MS.gene88520 | 0.886254 | 4.02E-72 | -1.69E-46 |
| MS.gene053213 | MS.gene88520 | 0.832671 | 8.27E-56 | -1.69E-46 |
| MS.gene053286 | MS.gene88520 | 0.82384 | 1.12E-53 | -1.69E-46 |
| MS.gene053322 | MS.gene88520 | 0.824059 | 9.91E-54 | -1.69E-46 |
| MS.gene053449 | MS.gene88520 | 0.8191 | 1.39E-52 | -1.69E-46 |
| MS.gene053632 | MS.gene88520 | 0.8034 | 3.55E-49 | -1.69E-46 |
| MS.gene053813 | MS.gene88520 | 0.826697 | 2.35E-54 | -1.69E-46 |
| MS.gene053928 | MS.gene88520 | 0.804902 | 1.73E-49 | -1.69E-46 |
| MS.gene054123 | MS.gene88520 | 0.820133 | 8.06E-53 | -1.69E-46 |
| MS.gene054376 | MS.gene88520 | 0.847126 | 1.41E-59 | -1.69E-46 |
| MS.gene054600 | MS.gene88520 | 0.822421 | 2.39E-53 | -1.69E-46 |
| MS.gene054802 | MS.gene88520 | 0.815102 | 1.10E-51 | -1.69E-46 |
| MS.gene054803 | MS.gene88520 | 0.814862 | 1.24E-51 | -1.69E-46 |
| MS.gene054804 | MS.gene88520 | 0.819115 | 1.38E-52 | -1.69E-46 |
| MS.gene05505 | MS.gene88520 | 0.815917 | 7.23E-52 | -1.69E-46 |
| MS.gene055116 | MS.gene88520 | 0.850875 | 1.28E-60 | -1.69E-46 |
| MS.gene055206 | MS.gene88520 | 0.804569 | 2.03E-49 | -1.69E-46 |
| MS.gene055277 | MS.gene88520 | 0.859028 | 5.49E-63 | -1.69E-46 |
| MS.gene055390 | MS.gene88520 | 0.814365 | 1.60E-51 | -1.69E-46 |
| MS.gene055397 | MS.gene88520 | 0.809501 | 1.83E-50 | -1.69E-46 |
| MS.gene055412 | MS.gene88520 | 0.820686 | 6.02E-53 | -1.69E-46 |
| MS.gene055428 | MS.gene88520 | 0.801531 | 8.62E-49 | -1.69E-46 |
| MS.gene055499 | MS.gene88520 | 0.836986 | 6.78E-57 | -1.69E-46 |
| MS.gene055564 | MS.gene88520 | 0.834554 | 2.80E-56 | -1.69E-46 |
| MS.gene055604 | MS.gene88520 | 0.802858 | 4.60E-49 | -1.69E-46 |
| MS.gene055647 | MS.gene88520 | 0.842279 | 2.85E-58 | -1.69E-46 |
| MS.gene055722 | MS.gene88520 | 0.815399 | 9.43E-52 | -1.69E-46 |
| MS.gene05592 | MS.gene88520 | 0.846779 | 1.75E-59 | -1.69E-46 |
| MS.gene055941 | MS.gene88520 | 0.809959 | 1.46E-50 | -1.69E-46 |
| MS.gene05602 | MS.gene88520 | 0.818205 | 2.21E-52 | -1.69E-46 |
| MS.gene056191 | MS.gene88520 | 0.85148 | 8.64E-61 | -1.69E-46 |
| MS.gene056374 | MS.gene88520 | 0.809439 | 1.89E-50 | -1.69E-46 |
| MS.gene056407 | MS.gene88520 | 0.857238 | 1.87E-62 | -1.69E-46 |
| MS.gene056410 | MS.gene88520 | 0.845227 | 4.63E-59 | -1.69E-46 |
| MS.gene056511 | MS.gene88520 | 0.816064 | 6.71E-52 | -1.69E-46 |
| MS.gene056609 | MS.gene88520 | 0.841214 | 5.44E-58 | -1.69E-46 |
| MS.gene05664 | MS.gene88520 | 0.816236 | 6.14E-52 | -1.69E-46 |
| MS.gene056659 | MS.gene88520 | 0.830699 | 2.54E-55 | -1.69E-46 |
| MS.gene05666 | MS.gene88520 | 0.813532 | 2.44E-51 | -1.69E-46 |
| MS.gene056796 | MS.gene88520 | 0.856021 | 4.26E-62 | -1.69E-46 |
| MS.gene056877 | MS.gene88520 | 0.812654 | 3.80E-51 | -1.69E-46 |
| MS.gene056897 | MS.gene88520 | 0.820552 | 6.46E-53 | -1.69E-46 |
| MS.gene057011 | MS.gene88520 | 0.82257 | 2.21E-53 | -1.69E-46 |
| MS.gene05702 | MS.gene88520 | 0.854472 | 1.20E-61 | -1.69E-46 |
| MS.gene057076 | MS.gene88520 | 0.812351 | 4.43E-51 | -1.69E-46 |
| MS.gene057124 | MS.gene88520 | 0.881204 | 2.92E-70 | -1.69E-46 |
| MS.gene057129 | MS.gene88520 | 0.829065 | 6.34E-55 | -1.69E-46 |
| MS.gene057196 | MS.gene88520 | 0.806649 | 7.42E-50 | -1.69E-46 |
| MS.gene057295 | MS.gene88520 | 0.81921 | 1.31E-52 | -1.69E-46 |
| MS.gene057331 | MS.gene88520 | 0.861724 | 8.39E-64 | -1.69E-46 |
| MS.gene05735 | MS.gene88520 | 0.805005 | 1.64E-49 | -1.69E-46 |
| MS.gene057416 | MS.gene88520 | 0.842557 | 2.41E-58 | -1.69E-46 |
| MS.gene057749 | MS.gene88520 | 0.840274 | 9.60E-58 | -1.69E-46 |
| MS.gene057823 | MS.gene88520 | 0.840496 | 8.40E-58 | -1.69E-46 |
| MS.gene058002 | MS.gene88520 | 0.819834 | 9.43E-53 | -1.69E-46 |
| MS.gene058006 | MS.gene88520 | 0.840041 | 1.10E-57 | -1.69E-46 |
| MS.gene058019 | MS.gene88520 | 0.846522 | 2.06E-59 | -1.69E-46 |
| MS.gene058082 | MS.gene88520 | 0.842555 | 2.41E-58 | -1.69E-46 |
| MS.gene05811 | MS.gene88520 | 0.801 | 1.11E-48 | -1.69E-46 |
| MS.gene058233 | MS.gene88520 | 0.830796 | 2.40E-55 | -1.69E-46 |
| MS.gene058293 | MS.gene88520 | 0.838073 | 3.57E-57 | -1.69E-46 |
| MS.gene058323 | MS.gene88520 | 0.818347 | 2.06E-52 | -1.69E-46 |
| MS.gene058365 | MS.gene88520 | 0.84051 | 8.33E-58 | -1.69E-46 |
| MS.gene058660 | MS.gene88520 | 0.85099 | 1.19E-60 | -1.69E-46 |
| MS.gene058700 | MS.gene88520 | 0.801368 | 9.30E-49 | -1.69E-46 |
| MS.gene058787 | MS.gene88520 | 0.824701 | 7.00E-54 | -1.69E-46 |
| MS.gene05886 | MS.gene88520 | 0.809249 | 2.08E-50 | -1.69E-46 |
| MS.gene058928 | MS.gene88520 | 0.806796 | 6.91E-50 | -1.69E-46 |
| MS.gene058948 | MS.gene88520 | 0.855602 | 5.65E-62 | -1.69E-46 |
| MS.gene058949 | MS.gene88520 | 0.802818 | 4.68E-49 | -1.69E-46 |
| MS.gene058993 | MS.gene88520 | 0.830624 | 2.64E-55 | -1.69E-46 |
| MS.gene05907 | MS.gene88520 | 0.826864 | 2.15E-54 | -1.69E-46 |
| MS.gene059366 | MS.gene88520 | 0.812005 | 5.27E-51 | -1.69E-46 |
| MS.gene059420 | MS.gene88520 | 0.804579 | 2.02E-49 | -1.69E-46 |
| MS.gene059536 | MS.gene88520 | 0.82379 | 1.15E-53 | -1.69E-46 |
| MS.gene059674 | MS.gene88520 | 0.801838 | 7.45E-49 | -1.69E-46 |
| MS.gene059676 | MS.gene88520 | 0.85121 | 1.03E-60 | -1.69E-46 |
| MS.gene059688 | MS.gene88520 | 0.863886 | 1.81E-64 | -1.69E-46 |
| MS.gene059692 | MS.gene88520 | 0.872829 | 2.35E-67 | -1.69E-46 |
| MS.gene059695 | MS.gene88520 | 0.874161 | 8.39E-68 | -1.69E-46 |
| MS.gene059711 | MS.gene88520 | 0.82614 | 3.19E-54 | -1.69E-46 |
| MS.gene059718 | MS.gene88520 | 0.805001 | 1.65E-49 | -1.69E-46 |
| MS.gene059960 | MS.gene88520 | 0.820239 | 7.62E-53 | -1.69E-46 |
| MS.gene059974 | MS.gene88520 | 0.817694 | 2.89E-52 | -1.69E-46 |
| MS.gene060193 | MS.gene88520 | 0.81857 | 1.83E-52 | -1.69E-46 |
| MS.gene060356 | MS.gene88520 | 0.806205 | 9.21E-50 | -1.69E-46 |
| MS.gene06037 | MS.gene88520 | 0.831805 | 1.36E-55 | -1.69E-46 |
| MS.gene060403 | MS.gene88520 | 0.844858 | 5.83E-59 | -1.69E-46 |
| MS.gene060569 | MS.gene88520 | 0.801641 | 8.18E-49 | -1.69E-46 |
| MS.gene060804 | MS.gene88520 | 0.855522 | 5.96E-62 | -1.69E-46 |
| MS.gene060835 | MS.gene88520 | 0.851639 | 7.79E-61 | -1.69E-46 |
| MS.gene060837 | MS.gene88520 | 0.856327 | 3.47E-62 | -1.69E-46 |
| MS.gene06092 | MS.gene88520 | 0.812251 | 4.66E-51 | -1.69E-46 |
| MS.gene060971 | MS.gene88520 | 0.819575 | 1.08E-52 | -1.69E-46 |
| MS.gene061041 | MS.gene88520 | 0.808351 | 3.23E-50 | -1.69E-46 |
| MS.gene061049 | MS.gene88520 | 0.832237 | 1.06E-55 | -1.69E-46 |
| MS.gene061051 | MS.gene88520 | -0.834559 | 2.80E-56 | -1.69E-46 |
| MS.gene061154 | MS.gene88520 | 0.833594 | 4.87E-56 | -1.69E-46 |
| MS.gene061200 | MS.gene88520 | 0.836314 | 1.01E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88520.t1 | MTR_1g076840 | 96.241 | 133 | 3 | 1 | 3 | 135 | 2 | 132 | 5.41e-91 | 259 |
| MS.gene88520.t1 | MTR_6g060170 | 82.474 | 97 | 17 | 0 | 39 | 135 | 2 | 98 | 1.70e-57 | 174 |
| MS.gene88520.t1 | MTR_1g069370 | 60.440 | 91 | 36 | 0 | 45 | 135 | 9 | 99 | 4.59e-38 | 125 |
| MS.gene88520.t1 | MTR_7g099200 | 59.341 | 91 | 37 | 0 | 45 | 135 | 5 | 95 | 2.70e-37 | 123 |
| MS.gene88520.t1 | MTR_6g059920 | 53.763 | 93 | 43 | 0 | 40 | 132 | 33 | 125 | 1.54e-30 | 106 |
| MS.gene88520.t1 | MTR_5g005820 | 51.087 | 92 | 45 | 0 | 40 | 131 | 2 | 93 | 2.45e-26 | 95.1 |
| MS.gene88520.t1 | MTR_6g060000 | 86.047 | 43 | 6 | 0 | 76 | 118 | 19 | 61 | 3.66e-22 | 83.6 |
| MS.gene88520.t1 | MTR_6g060090 | 68.000 | 50 | 14 | 1 | 68 | 117 | 9 | 56 | 2.21e-18 | 73.6 |
| MS.gene88520.t1 | MTR_1g086000 | 32.500 | 80 | 54 | 0 | 37 | 116 | 39 | 118 | 1.41e-12 | 61.2 |
| MS.gene88520.t1 | MTR_6g060030 | 55.172 | 58 | 8 | 1 | 96 | 135 | 7 | 64 | 1.63e-12 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88520.t1 | AT4G10300 | 67.669 | 133 | 39 | 3 | 6 | 135 | 3 | 134 | 1.03e-59 | 181 |
| MS.gene88520.t1 | AT3G04300 | 56.044 | 91 | 40 | 0 | 45 | 135 | 1 | 91 | 1.65e-35 | 118 |
| MS.gene88520.t1 | AT4G28703 | 48.454 | 97 | 41 | 1 | 47 | 134 | 8 | 104 | 4.92e-29 | 102 |
| MS.gene88520.t1 | AT4G10280 | 48.454 | 97 | 47 | 1 | 42 | 135 | 43 | 139 | 1.55e-26 | 97.1 |
| MS.gene88520.t1 | AT4G10290 | 43.617 | 94 | 50 | 1 | 42 | 132 | 15 | 108 | 3.28e-20 | 80.1 |
| MS.gene88520.t1 | AT2G32180 | 30.380 | 79 | 55 | 0 | 41 | 119 | 40 | 118 | 9.89e-12 | 58.9 |
| MS.gene88520.t1 | AT2G32180 | 30.380 | 79 | 55 | 0 | 41 | 119 | 40 | 118 | 9.89e-12 | 58.9 |
| MS.gene88520.t1 | AT2G32650 | 30.380 | 79 | 55 | 0 | 41 | 119 | 40 | 118 | 1.09e-11 | 58.5 |
| MS.gene88520.t1 | AT2G32650 | 30.380 | 79 | 55 | 0 | 41 | 119 | 40 | 118 | 1.09e-11 | 58.5 |
Find 34 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAGTACAACTCATCCCTTT+TGG | 0.324093 | 1.2:+63639575 | None:intergenic |
| TGAAATTTCTGCTGGTGATT+TGG | 0.359064 | 1.2:-63639606 | MS.gene88520:CDS |
| AACAGAGACATGCTATCTTT+TGG | 0.368243 | 1.2:-63639672 | MS.gene88520:CDS |
| CAAAAGGGATGAGTTGTACT+TGG | 0.379437 | 1.2:-63639574 | MS.gene88520:CDS |
| CTTGGTAGCAGTCAAATGTT+TGG | 0.389927 | 1.2:+63640280 | None:intergenic |
| ACAACTCTTCTCCTTGTTGA+AGG | 0.406999 | 1.2:+63640236 | None:intergenic |
| AGCTCACTCAACTTGGTGTT+AGG | 0.443465 | 1.2:-63640154 | MS.gene88520:CDS |
| AACACCAAGTTGAGTGAGCT+TGG | 0.455326 | 1.2:+63640157 | None:intergenic |
| AGTTGAGTGAGCTTGGACTC+AGG | 0.458878 | 1.2:+63640164 | None:intergenic |
| GGCAAAGTTACACTTACCTT+GGG | 0.470688 | 1.2:+63640257 | None:intergenic |
| CACTCCTAGTGGGGCTAATG+AGG | 0.472036 | 1.2:-63639633 | MS.gene88520:CDS |
| TATGTCCATGGAAAATTACT+TGG | 0.480875 | 1.2:+63639701 | None:intergenic |
| CTCAACTTGGTGTTAGGCAG+TGG | 0.492268 | 1.2:-63640148 | MS.gene88520:CDS |
| AGGCAAAGTTACACTTACCT+TGG | 0.501016 | 1.2:+63640256 | None:intergenic |
| TGTACTTGGGATGTGTCTGT+TGG | 0.525073 | 1.2:-63639560 | MS.gene88520:CDS |
| GAGTCCAAGCTCACTCAACT+TGG | 0.526860 | 1.2:-63640161 | MS.gene88520:CDS |
| AGTTACACTTACCTTGGGCT+TGG | 0.544555 | 1.2:+63640262 | None:intergenic |
| TTGGGATGTGTCTGTTGGTG+TGG | 0.547160 | 1.2:-63639555 | MS.gene88520:CDS |
| AGTCAAATGTTTGGTTTGTG+AGG | 0.547845 | 1.2:+63640289 | None:intergenic |
| GTCCATGGAAAATTACTTGG+AGG | 0.553197 | 1.2:+63639704 | None:intergenic |
| GCAGCTGTCACAGAGAAACT+TGG | 0.559175 | 1.2:-63640209 | MS.gene88520:CDS |
| AAGTGAAGGTCACTCCTAGT+GGG | 0.564562 | 1.2:-63639643 | MS.gene88520:CDS |
| TCCATGGAAAATTACTTGGA+GGG | 0.566600 | 1.2:+63639705 | None:intergenic |
| AAAGTGAAGGTCACTCCTAG+TGG | 0.587646 | 1.2:-63639644 | MS.gene88520:CDS |
| GAGGCAGTTGAAATTTCTGC+TGG | 0.589901 | 1.2:-63639614 | MS.gene88520:CDS |
| TGAGTGAGCTTGGACTCAGG+AGG | 0.606187 | 1.2:+63640167 | None:intergenic |
| AAAAGGGATGAGTTGTACTT+GGG | 0.609307 | 1.2:-63639573 | MS.gene88520:CDS |
| ATGGCATCATCATCATGTGT+AGG | 0.613363 | 1.2:-63640323 | MS.gene88520:CDS |
| GTGTAACTTTGCCTTCAACA+AGG | 0.618419 | 1.2:-63640247 | MS.gene88520:CDS |
| TCTGTTGCATCATATGTCCA+TGG | 0.622988 | 1.2:+63639689 | None:intergenic |
| GAGTGAGCTTGGACTCAGGA+GGG | 0.632998 | 1.2:+63640168 | None:intergenic |
| ACTGCCTCATTAGCCCCACT+AGG | 0.662701 | 1.2:+63639629 | None:intergenic |
| TTTGACTGCTACCAAGCCCA+AGG | 0.672230 | 1.2:-63640273 | MS.gene88520:CDS |
| AGTGAAGGTCACTCCTAGTG+GGG | 0.682734 | 1.2:-63639642 | MS.gene88520:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GATTTTTATTTTAATGAAAA+AGG | - | chr1.2:63639839-63639858 | MS.gene88520:intron | 10.0% |
| !! | ACAACATAAAAAATCTTTCA+CGG | - | chr1.2:63640040-63640059 | MS.gene88520:intron | 20.0% |
| !! | TACAGTTAAAGTCATATATA+TGG | + | chr1.2:63639963-63639982 | None:intergenic | 20.0% |
| !!! | AAACGTTTTTTATGATTTGA+CGG | - | chr1.2:63639998-63640017 | MS.gene88520:intron | 20.0% |
| ! | AAGAAATGTGGTATTTACTT+GGG | + | chr1.2:63639733-63639752 | None:intergenic | 25.0% |
| ! | AAGAAGATAATGAAGAAATG+TGG | + | chr1.2:63639745-63639764 | None:intergenic | 25.0% |
| ! | ATATGACTTTAACTGTAGTT+AGG | - | chr1.2:63639966-63639985 | MS.gene88520:intron | 25.0% |
| ! | TGGTGTAAATAAAAACAGAT+GGG | - | chr1.2:63640123-63640142 | MS.gene88520:intron | 25.0% |
| ! | TTAACGAGATTAATACACTA+TGG | - | chr1.2:63639917-63639936 | MS.gene88520:intron | 25.0% |
| ! | TTAGAATAATAGACATGATG+AGG | + | chr1.2:63639776-63639795 | None:intergenic | 25.0% |
| ! | TTGGTGTAAATAAAAACAGA+TGG | - | chr1.2:63640122-63640141 | MS.gene88520:intron | 25.0% |
| !!! | GTTTCTTACATTTTTTCTCA+TGG | - | chr1.2:63639804-63639823 | MS.gene88520:intron | 25.0% |
| GAAGAAATGTGGTATTTACT+TGG | + | chr1.2:63639734-63639753 | None:intergenic | 30.0% | |
| GGTGTAAATAAAAACAGATG+GGG | - | chr1.2:63640124-63640143 | MS.gene88520:intron | 30.0% | |
| TATGTCCATGGAAAATTACT+TGG | + | chr1.2:63640157-63640176 | None:intergenic | 30.0% | |
| !! | ATTTGGTTGTTTTCCCAAAA+GGG | - | chr1.2:63640266-63640285 | MS.gene88520:CDS | 30.0% |
| !!! | TCTTACATTTTTTCTCATGG+TGG | - | chr1.2:63639807-63639826 | MS.gene88520:intron | 30.0% |
| AAAAGGGATGAGTTGTACTT+GGG | - | chr1.2:63640282-63640301 | MS.gene88520:CDS | 35.0% | |
| TCCATGGAAAATTACTTGGA+GGG | + | chr1.2:63640153-63640172 | None:intergenic | 35.0% | |
| TGAAATTTCTGCTGGTGATT+TGG | - | chr1.2:63640249-63640268 | MS.gene88520:CDS | 35.0% | |
| ! | AACAGAGACATGCTATCTTT+TGG | - | chr1.2:63640183-63640202 | MS.gene88520:CDS | 35.0% |
| ! | AAGTACAACTCATCCCTTTT+GGG | + | chr1.2:63640282-63640301 | None:intergenic | 35.0% |
| !! | AGTCAAATGTTTGGTTTGTG+AGG | + | chr1.2:63639569-63639588 | None:intergenic | 35.0% |
| !! | GATTTGGTTGTTTTCCCAAA+AGG | - | chr1.2:63640265-63640284 | MS.gene88520:CDS | 35.0% |
| !! | TCTTTTGGAAGGAAAAGTGA+AGG | - | chr1.2:63640198-63640217 | MS.gene88520:CDS | 35.0% |
| ACAACTCTTCTCCTTGTTGA+AGG | + | chr1.2:63639622-63639641 | None:intergenic | 40.0% | |
| ACTAGAAGAGCAACTAGCAT+AGG | + | chr1.2:63639898-63639917 | None:intergenic | 40.0% | |
| AGGCAAAGTTACACTTACCT+TGG | + | chr1.2:63639602-63639621 | None:intergenic | 40.0% | |
| ATGGCATCATCATCATGTGT+AGG | - | chr1.2:63639532-63639551 | MS.gene88520:CDS | 40.0% | |
| CAAAAGGGATGAGTTGTACT+TGG | - | chr1.2:63640281-63640300 | MS.gene88520:CDS | 40.0% | |
| CTAGAAGAGCAACTAGCATA+GGG | + | chr1.2:63639897-63639916 | None:intergenic | 40.0% | |
| GGCAAAGTTACACTTACCTT+GGG | + | chr1.2:63639601-63639620 | None:intergenic | 40.0% | |
| GTCCATGGAAAATTACTTGG+AGG | + | chr1.2:63640154-63640173 | None:intergenic | 40.0% | |
| GTGTAACTTTGCCTTCAACA+AGG | - | chr1.2:63639608-63639627 | MS.gene88520:CDS | 40.0% | |
| TCTGTTGCATCATATGTCCA+TGG | + | chr1.2:63640169-63640188 | None:intergenic | 40.0% | |
| ! | CAAGTACAACTCATCCCTTT+TGG | + | chr1.2:63640283-63640302 | None:intergenic | 40.0% |
| ! | CTTGGTAGCAGTCAAATGTT+TGG | + | chr1.2:63639578-63639597 | None:intergenic | 40.0% |
| ! | GAGACATGCTATCTTTTGGA+AGG | - | chr1.2:63640187-63640206 | MS.gene88520:CDS | 40.0% |
| ! | TGACTGTGTACGCACATTTT+TGG | - | chr1.2:63640103-63640122 | MS.gene88520:intron | 40.0% |
| AAAGTGAAGGTCACTCCTAG+TGG | - | chr1.2:63640211-63640230 | MS.gene88520:CDS | 45.0% | |
| AAGTGAAGGTCACTCCTAGT+GGG | - | chr1.2:63640212-63640231 | MS.gene88520:CDS | 45.0% | |
| AGTTACACTTACCTTGGGCT+TGG | + | chr1.2:63639596-63639615 | None:intergenic | 45.0% | |
| GAGGCAGTTGAAATTTCTGC+TGG | - | chr1.2:63640241-63640260 | MS.gene88520:CDS | 45.0% | |
| TGTACTTGGGATGTGTCTGT+TGG | - | chr1.2:63640295-63640314 | MS.gene88520:CDS | 45.0% | |
| ! | AACACCAAGTTGAGTGAGCT+TGG | + | chr1.2:63639701-63639720 | None:intergenic | 45.0% |
| ! | GCCCTCCAAGTAATTTTCCA+TGG | - | chr1.2:63640149-63640168 | MS.gene88520:CDS | 45.0% |
| !! | AGCTCACTCAACTTGGTGTT+AGG | - | chr1.2:63639701-63639720 | MS.gene88520:CDS | 45.0% |
| AGTGAAGGTCACTCCTAGTG+GGG | - | chr1.2:63640213-63640232 | MS.gene88520:CDS | 50.0% | |
| AGTTGAGTGAGCTTGGACTC+AGG | + | chr1.2:63639694-63639713 | None:intergenic | 50.0% | |
| GAGTCCAAGCTCACTCAACT+TGG | - | chr1.2:63639694-63639713 | MS.gene88520:CDS | 50.0% | |
| GCAGCTGTCACAGAGAAACT+TGG | - | chr1.2:63639646-63639665 | MS.gene88520:CDS | 50.0% | |
| TTGGGATGTGTCTGTTGGTG+TGG | - | chr1.2:63640300-63640319 | MS.gene88520:CDS | 50.0% | |
| TTTGACTGCTACCAAGCCCA+AGG | - | chr1.2:63639582-63639601 | MS.gene88520:CDS | 50.0% | |
| !! | CTCAACTTGGTGTTAGGCAG+TGG | - | chr1.2:63639707-63639726 | MS.gene88520:CDS | 50.0% |
| ACTGCCTCATTAGCCCCACT+AGG | + | chr1.2:63640229-63640248 | None:intergenic | 55.0% | |
| CACTCCTAGTGGGGCTAATG+AGG | - | chr1.2:63640222-63640241 | MS.gene88520:CDS | 55.0% | |
| GAGTGAGCTTGGACTCAGGA+GGG | + | chr1.2:63639690-63639709 | None:intergenic | 55.0% | |
| TGAGTGAGCTTGGACTCAGG+AGG | + | chr1.2:63639691-63639710 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 63639532 | 63640345 | 63639532 | ID=MS.gene88520 |
| chr1.2 | mRNA | 63639532 | 63640345 | 63639532 | ID=MS.gene88520.t1;Parent=MS.gene88520 |
| chr1.2 | exon | 63640143 | 63640345 | 63640143 | ID=MS.gene88520.t1.exon1;Parent=MS.gene88520.t1 |
| chr1.2 | CDS | 63640143 | 63640345 | 63640143 | ID=cds.MS.gene88520.t1;Parent=MS.gene88520.t1 |
| chr1.2 | exon | 63639532 | 63639736 | 63639532 | ID=MS.gene88520.t1.exon2;Parent=MS.gene88520.t1 |
| chr1.2 | CDS | 63639532 | 63639736 | 63639532 | ID=cds.MS.gene88520.t1;Parent=MS.gene88520.t1 |
| Gene Sequence |
| Protein sequence |
