Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90810.t1 | XP_003592756.1 | 95.3 | 191 | 8 | 1 | 1 | 190 | 1 | 191 | 1.20E-91 | 345.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90810.t1 | B7FM34 | 95.3 | 191 | 8 | 1 | 1 | 190 | 1 | 191 | 8.6e-92 | 345.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene90810 | 0.834416 | 3.04E-56 | -1.69E-46 |
| MS.gene049171 | MS.gene90810 | 0.809481 | 1.85E-50 | -1.69E-46 |
| MS.gene049222 | MS.gene90810 | 0.828109 | 1.08E-54 | -1.69E-46 |
| MS.gene049285 | MS.gene90810 | 0.802817 | 4.69E-49 | -1.69E-46 |
| MS.gene049427 | MS.gene90810 | 0.869093 | 4.01E-66 | -1.69E-46 |
| MS.gene049609 | MS.gene90810 | 0.849768 | 2.62E-60 | -1.69E-46 |
| MS.gene049661 | MS.gene90810 | 0.820775 | 5.74E-53 | -1.69E-46 |
| MS.gene049662 | MS.gene90810 | 0.811464 | 6.91E-51 | -1.69E-46 |
| MS.gene049805 | MS.gene90810 | 0.882622 | 8.93E-71 | -1.69E-46 |
| MS.gene050076 | MS.gene90810 | 0.815359 | 9.63E-52 | -1.69E-46 |
| MS.gene050163 | MS.gene90810 | 0.811573 | 6.54E-51 | -1.69E-46 |
| MS.gene050301 | MS.gene90810 | 0.813959 | 1.97E-51 | -1.69E-46 |
| MS.gene050397 | MS.gene90810 | 0.802554 | 5.31E-49 | -1.69E-46 |
| MS.gene050432 | MS.gene90810 | 0.842736 | 2.15E-58 | -1.69E-46 |
| MS.gene050464 | MS.gene90810 | 0.801485 | 8.81E-49 | -1.69E-46 |
| MS.gene050518 | MS.gene90810 | 0.800222 | 1.59E-48 | -1.69E-46 |
| MS.gene050529 | MS.gene90810 | 0.8001 | 1.69E-48 | -1.69E-46 |
| MS.gene050538 | MS.gene90810 | 0.805826 | 1.11E-49 | -1.69E-46 |
| MS.gene050593 | MS.gene90810 | 0.868912 | 4.59E-66 | -1.69E-46 |
| MS.gene050670 | MS.gene90810 | 0.86929 | 3.46E-66 | -1.69E-46 |
| MS.gene050699 | MS.gene90810 | 0.80122 | 9.98E-49 | -1.69E-46 |
| MS.gene050747 | MS.gene90810 | 0.851754 | 7.22E-61 | -1.69E-46 |
| MS.gene050767 | MS.gene90810 | 0.824528 | 7.69E-54 | -1.69E-46 |
| MS.gene050770 | MS.gene90810 | 0.827057 | 1.93E-54 | -1.69E-46 |
| MS.gene050772 | MS.gene90810 | 0.849364 | 3.39E-60 | -1.69E-46 |
| MS.gene050783 | MS.gene90810 | 0.813777 | 2.16E-51 | -1.69E-46 |
| MS.gene050950 | MS.gene90810 | 0.852058 | 5.92E-61 | -1.69E-46 |
| MS.gene05101 | MS.gene90810 | 0.845471 | 3.98E-59 | -1.69E-46 |
| MS.gene05105 | MS.gene90810 | 0.853578 | 2.18E-61 | -1.69E-46 |
| MS.gene051228 | MS.gene90810 | 0.826741 | 2.30E-54 | -1.69E-46 |
| MS.gene051387 | MS.gene90810 | 0.862329 | 5.47E-64 | -1.69E-46 |
| MS.gene051582 | MS.gene90810 | 0.846982 | 1.54E-59 | -1.69E-46 |
| MS.gene051666 | MS.gene90810 | 0.891221 | 4.84E-74 | -1.69E-46 |
| MS.gene051692 | MS.gene90810 | 0.836365 | 9.76E-57 | -1.69E-46 |
| MS.gene051810 | MS.gene90810 | 0.805303 | 1.42E-49 | -1.69E-46 |
| MS.gene052078 | MS.gene90810 | 0.805261 | 1.45E-49 | -1.69E-46 |
| MS.gene052188 | MS.gene90810 | 0.808442 | 3.09E-50 | -1.69E-46 |
| MS.gene052271 | MS.gene90810 | 0.810189 | 1.30E-50 | -1.69E-46 |
| MS.gene052290 | MS.gene90810 | 0.81132 | 7.43E-51 | -1.69E-46 |
| MS.gene052310 | MS.gene90810 | 0.823344 | 1.46E-53 | -1.69E-46 |
| MS.gene052322 | MS.gene90810 | 0.849034 | 4.19E-60 | -1.69E-46 |
| MS.gene052340 | MS.gene90810 | 0.814703 | 1.35E-51 | -1.69E-46 |
| MS.gene052421 | MS.gene90810 | 0.834148 | 3.54E-56 | -1.69E-46 |
| MS.gene052481 | MS.gene90810 | 0.852505 | 4.42E-61 | -1.69E-46 |
| MS.gene052484 | MS.gene90810 | 0.845465 | 3.99E-59 | -1.69E-46 |
| MS.gene052485 | MS.gene90810 | 0.823845 | 1.11E-53 | -1.69E-46 |
| MS.gene052501 | MS.gene90810 | 0.801992 | 6.93E-49 | -1.69E-46 |
| MS.gene052505 | MS.gene90810 | 0.800714 | 1.27E-48 | -1.69E-46 |
| MS.gene052615 | MS.gene90810 | 0.833166 | 6.23E-56 | -1.69E-46 |
| MS.gene052982 | MS.gene90810 | 0.829443 | 5.13E-55 | -1.69E-46 |
| MS.gene053009 | MS.gene90810 | 0.842062 | 3.25E-58 | -1.69E-46 |
| MS.gene053103 | MS.gene90810 | 0.857732 | 1.34E-62 | -1.69E-46 |
| MS.gene053104 | MS.gene90810 | 0.860592 | 1.86E-63 | -1.69E-46 |
| MS.gene053107 | MS.gene90810 | 0.822854 | 1.90E-53 | -1.69E-46 |
| MS.gene053207 | MS.gene90810 | 0.861195 | 1.22E-63 | -1.69E-46 |
| MS.gene053209 | MS.gene90810 | 0.843068 | 1.76E-58 | -1.69E-46 |
| MS.gene053213 | MS.gene90810 | 0.850062 | 2.17E-60 | -1.69E-46 |
| MS.gene053286 | MS.gene90810 | 0.838839 | 2.27E-57 | -1.69E-46 |
| MS.gene053322 | MS.gene90810 | 0.877205 | 7.57E-69 | -1.69E-46 |
| MS.gene053436 | MS.gene90810 | 0.811257 | 7.67E-51 | -1.69E-46 |
| MS.gene053449 | MS.gene90810 | 0.809952 | 1.47E-50 | -1.69E-46 |
| MS.gene053574 | MS.gene90810 | 0.831577 | 1.54E-55 | -1.69E-46 |
| MS.gene053579 | MS.gene90810 | 0.820089 | 8.25E-53 | -1.69E-46 |
| MS.gene053632 | MS.gene90810 | 0.834932 | 2.25E-56 | -1.69E-46 |
| MS.gene053912 | MS.gene90810 | 0.8089 | 2.47E-50 | -1.69E-46 |
| MS.gene054123 | MS.gene90810 | 0.830666 | 2.58E-55 | -1.69E-46 |
| MS.gene054376 | MS.gene90810 | 0.838976 | 2.09E-57 | -1.69E-46 |
| MS.gene054408 | MS.gene90810 | 0.807879 | 4.07E-50 | -1.69E-46 |
| MS.gene054600 | MS.gene90810 | 0.830705 | 2.53E-55 | -1.69E-46 |
| MS.gene054642 | MS.gene90810 | 0.838399 | 2.94E-57 | -1.69E-46 |
| MS.gene054802 | MS.gene90810 | 0.803551 | 3.30E-49 | -1.69E-46 |
| MS.gene054803 | MS.gene90810 | 0.80259 | 5.22E-49 | -1.69E-46 |
| MS.gene054804 | MS.gene90810 | 0.806618 | 7.54E-50 | -1.69E-46 |
| MS.gene054927 | MS.gene90810 | 0.810479 | 1.13E-50 | -1.69E-46 |
| MS.gene05505 | MS.gene90810 | 0.825561 | 4.38E-54 | -1.69E-46 |
| MS.gene055116 | MS.gene90810 | 0.861846 | 7.70E-64 | -1.69E-46 |
| MS.gene055206 | MS.gene90810 | 0.826836 | 2.18E-54 | -1.69E-46 |
| MS.gene055289 | MS.gene90810 | 0.807717 | 4.41E-50 | -1.69E-46 |
| MS.gene055397 | MS.gene90810 | 0.831952 | 1.25E-55 | -1.69E-46 |
| MS.gene055412 | MS.gene90810 | 0.841453 | 4.71E-58 | -1.69E-46 |
| MS.gene055499 | MS.gene90810 | 0.857475 | 1.59E-62 | -1.69E-46 |
| MS.gene055502 | MS.gene90810 | 0.800241 | 1.58E-48 | -1.69E-46 |
| MS.gene055577 | MS.gene90810 | 0.810324 | 1.22E-50 | -1.69E-46 |
| MS.gene055647 | MS.gene90810 | 0.84791 | 8.57E-60 | -1.69E-46 |
| MS.gene055722 | MS.gene90810 | 0.83416 | 3.52E-56 | -1.69E-46 |
| MS.gene05592 | MS.gene90810 | 0.872224 | 3.75E-67 | -1.69E-46 |
| MS.gene055941 | MS.gene90810 | 0.81114 | 8.12E-51 | -1.69E-46 |
| MS.gene056083 | MS.gene90810 | 0.825034 | 5.84E-54 | -1.69E-46 |
| MS.gene056374 | MS.gene90810 | 0.855476 | 6.15E-62 | -1.69E-46 |
| MS.gene056407 | MS.gene90810 | 0.846436 | 2.18E-59 | -1.69E-46 |
| MS.gene056511 | MS.gene90810 | 0.830007 | 3.74E-55 | -1.69E-46 |
| MS.gene056609 | MS.gene90810 | 0.830841 | 2.34E-55 | -1.69E-46 |
| MS.gene05664 | MS.gene90810 | 0.849159 | 3.87E-60 | -1.69E-46 |
| MS.gene056657 | MS.gene90810 | 0.801246 | 9.86E-49 | -1.69E-46 |
| MS.gene05666 | MS.gene90810 | 0.835373 | 1.74E-56 | -1.69E-46 |
| MS.gene056897 | MS.gene90810 | 0.866188 | 3.42E-65 | -1.69E-46 |
| MS.gene057011 | MS.gene90810 | 0.849598 | 2.92E-60 | -1.69E-46 |
| MS.gene05702 | MS.gene90810 | 0.821011 | 5.06E-53 | -1.69E-46 |
| MS.gene057124 | MS.gene90810 | 0.870136 | 1.83E-66 | -1.69E-46 |
| MS.gene057129 | MS.gene90810 | 0.867872 | 9.94E-66 | -1.69E-46 |
| MS.gene057146 | MS.gene90810 | 0.82706 | 1.93E-54 | -1.69E-46 |
| MS.gene057295 | MS.gene90810 | 0.841939 | 3.50E-58 | -1.69E-46 |
| MS.gene057331 | MS.gene90810 | 0.87247 | 3.10E-67 | -1.69E-46 |
| MS.gene05735 | MS.gene90810 | 0.814869 | 1.24E-51 | -1.69E-46 |
| MS.gene057416 | MS.gene90810 | 0.888626 | 4.99E-73 | -1.69E-46 |
| MS.gene057449 | MS.gene90810 | 0.801666 | 8.09E-49 | -1.69E-46 |
| MS.gene057734 | MS.gene90810 | 0.822227 | 2.65E-53 | -1.69E-46 |
| MS.gene058006 | MS.gene90810 | 0.847716 | 9.70E-60 | -1.69E-46 |
| MS.gene058019 | MS.gene90810 | 0.860049 | 2.71E-63 | -1.69E-46 |
| MS.gene058082 | MS.gene90810 | 0.817627 | 2.99E-52 | -1.69E-46 |
| MS.gene058233 | MS.gene90810 | 0.817909 | 2.58E-52 | -1.69E-46 |
| MS.gene058293 | MS.gene90810 | 0.849081 | 4.07E-60 | -1.69E-46 |
| MS.gene058323 | MS.gene90810 | 0.824201 | 9.18E-54 | -1.69E-46 |
| MS.gene058419 | MS.gene90810 | 0.820585 | 6.34E-53 | -1.69E-46 |
| MS.gene058544 | MS.gene90810 | 0.834742 | 2.51E-56 | -1.69E-46 |
| MS.gene058660 | MS.gene90810 | 0.855416 | 6.40E-62 | -1.69E-46 |
| MS.gene058700 | MS.gene90810 | 0.815049 | 1.13E-51 | -1.69E-46 |
| MS.gene058723 | MS.gene90810 | 0.815029 | 1.14E-51 | -1.69E-46 |
| MS.gene058787 | MS.gene90810 | 0.818161 | 2.26E-52 | -1.69E-46 |
| MS.gene058838 | MS.gene90810 | 0.814223 | 1.72E-51 | -1.69E-46 |
| MS.gene05883 | MS.gene90810 | 0.816546 | 5.23E-52 | -1.69E-46 |
| MS.gene058928 | MS.gene90810 | 0.806179 | 9.33E-50 | -1.69E-46 |
| MS.gene05934 | MS.gene90810 | 0.812799 | 3.54E-51 | -1.69E-46 |
| MS.gene059455 | MS.gene90810 | 0.800566 | 1.36E-48 | -1.69E-46 |
| MS.gene059536 | MS.gene90810 | 0.869947 | 2.11E-66 | -1.69E-46 |
| MS.gene059597 | MS.gene90810 | 0.803001 | 4.29E-49 | -1.69E-46 |
| MS.gene05959 | MS.gene90810 | 0.803984 | 2.68E-49 | -1.69E-46 |
| MS.gene059676 | MS.gene90810 | 0.84218 | 3.03E-58 | -1.69E-46 |
| MS.gene059688 | MS.gene90810 | 0.876521 | 1.31E-68 | -1.69E-46 |
| MS.gene059692 | MS.gene90810 | 0.86735 | 1.46E-65 | -1.69E-46 |
| MS.gene059695 | MS.gene90810 | 0.866855 | 2.10E-65 | -1.69E-46 |
| MS.gene059718 | MS.gene90810 | 0.821671 | 3.57E-53 | -1.69E-46 |
| MS.gene059960 | MS.gene90810 | 0.864689 | 1.01E-64 | -1.69E-46 |
| MS.gene060193 | MS.gene90810 | 0.803151 | 4.00E-49 | -1.69E-46 |
| MS.gene060403 | MS.gene90810 | 0.875236 | 3.61E-68 | -1.69E-46 |
| MS.gene060804 | MS.gene90810 | 0.859656 | 3.56E-63 | -1.69E-46 |
| MS.gene060818 | MS.gene90810 | 0.824629 | 7.28E-54 | -1.69E-46 |
| MS.gene060835 | MS.gene90810 | 0.817895 | 2.60E-52 | -1.69E-46 |
| MS.gene060837 | MS.gene90810 | 0.825219 | 5.28E-54 | -1.69E-46 |
| MS.gene06092 | MS.gene90810 | 0.858328 | 8.88E-63 | -1.69E-46 |
| MS.gene061041 | MS.gene90810 | 0.824566 | 7.53E-54 | -1.69E-46 |
| MS.gene061049 | MS.gene90810 | 0.805035 | 1.62E-49 | -1.69E-46 |
| MS.gene061051 | MS.gene90810 | -0.854502 | 1.18E-61 | -1.69E-46 |
| MS.gene061150 | MS.gene90810 | 0.831529 | 1.59E-55 | -1.69E-46 |
| MS.gene061200 | MS.gene90810 | 0.862632 | 4.42E-64 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90810.t1 | MTR_1g115740 | 95.288 | 191 | 8 | 1 | 1 | 190 | 1 | 191 | 1.92e-115 | 326 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90810.t1 | AT3G12345 | 53.125 | 192 | 74 | 6 | 4 | 185 | 1 | 186 | 9.18e-49 | 157 |
Find 66 sgRNAs with CRISPR-Local
Find 71 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTCGAAGTCTTCTCCGGATT+CGG | 0.259014 | 1.4:-87993118 | None:intergenic |
| TCGGATTTCTGCTTTCTTGC+CGG | 0.273376 | 1.4:-87993181 | None:intergenic |
| GGTGGGTTGAAGGTGGAGTT+AGG | 0.286204 | 1.4:+87993294 | MS.gene90810:CDS |
| GTCTTCTCCGGATTCGGGTT+TGG | 0.296851 | 1.4:-87993112 | None:intergenic |
| TCGAAGTCTTCTCCGGATTC+GGG | 0.304678 | 1.4:-87993117 | None:intergenic |
| CCGCTGAAAGAACCGGTTTC+CGG | 0.306728 | 1.4:+87993273 | MS.gene90810:CDS |
| TCGGGGTCGGGACCGGGTTC+CGG | 0.325614 | 1.4:+87993231 | MS.gene90810:CDS |
| TTAGTTGAAGAAGAATTATC+AGG | 0.339500 | 1.4:-87993513 | None:intergenic |
| GAAGAAGAAGAAAGTGGAAA+TGG | 0.354465 | 1.4:-87993006 | None:intergenic |
| GGCTACATCGGGGTCGGGAC+CGG | 0.376853 | 1.4:+87993224 | MS.gene90810:CDS |
| AATGGTAGAATTGCATTACT+TGG | 0.377104 | 1.4:+87993342 | MS.gene90810:CDS |
| AATTGCATTACTTGGATTAA+CGG | 0.390474 | 1.4:+87993350 | MS.gene90810:CDS |
| TTGTTGGTGGAGCTTGCAAC+TGG | 0.404741 | 1.4:+87993378 | MS.gene90810:CDS |
| AAATCTAACAAGGCTACATC+GGG | 0.409096 | 1.4:+87993213 | MS.gene90810:CDS |
| GGAGGGTGGTGTTTGAGGTT+CGG | 0.426231 | 1.4:-87993076 | None:intergenic |
| GAGTCGGAGGGTGGTGTTTG+AGG | 0.427392 | 1.4:-87993081 | None:intergenic |
| GGGTCTCCGGAGAAAAGATT+TGG | 0.433276 | 1.4:-87993028 | None:intergenic |
| ACCGGTTCTTTCAGCGGCAC+CGG | 0.435063 | 1.4:-87993267 | None:intergenic |
| GCCGGTGCCGCTGAAAGAAC+CGG | 0.443892 | 1.4:+87993266 | MS.gene90810:CDS |
| ACCGGTTTCCGGTGGGTTGA+AGG | 0.446296 | 1.4:+87993284 | MS.gene90810:CDS |
| TCTCCGGATTCGGGTTTGGA+TGG | 0.448986 | 1.4:-87993108 | None:intergenic |
| ACCTTCAACCCACCGGAAAC+CGG | 0.463956 | 1.4:-87993285 | None:intergenic |
| GCTACATCGGGGTCGGGACC+GGG | 0.464069 | 1.4:+87993225 | MS.gene90810:CDS |
| TGGATTAACGGCACTGTTGT+TGG | 0.466065 | 1.4:+87993362 | MS.gene90810:CDS |
| AAGAAGAAGAAAGTGGAAAT+GGG | 0.484348 | 1.4:-87993005 | None:intergenic |
| GATCCATCCAAACCCGAATC+CGG | 0.485651 | 1.4:+87993105 | MS.gene90810:CDS |
| AGATACAGTGAGAGATTGAA+TGG | 0.486900 | 1.4:+87993324 | MS.gene90810:CDS |
| GTGGGTTGAAGGTGGAGTTA+GGG | 0.495100 | 1.4:+87993295 | MS.gene90810:CDS |
| AACAAGGCTACATCGGGGTC+GGG | 0.495458 | 1.4:+87993219 | MS.gene90810:CDS |
| AAAATCTAACAAGGCTACAT+CGG | 0.499533 | 1.4:+87993212 | MS.gene90810:CDS |
| TGAAGAGAAGGAAGGGTCTC+CGG | 0.503944 | 1.4:-87993041 | None:intergenic |
| CTGAAAGAACCGGTTTCCGG+TGG | 0.509695 | 1.4:+87993276 | MS.gene90810:CDS |
| TGCCGGTGCCTCTCTTGTAA+CGG | 0.509867 | 1.4:-87993164 | None:intergenic |
| TGCCTCTCTTGTAACGGATA+CGG | 0.530086 | 1.4:-87993158 | None:intergenic |
| GTGGAGCTTGCAACTGGAAA+AGG | 0.541105 | 1.4:+87993384 | MS.gene90810:CDS |
| ATCCGTTACAAGAGAGGCAC+CGG | 0.544488 | 1.4:+87993162 | MS.gene90810:CDS |
| AAGAAAGCAGAAATCCGAAA+AGG | 0.544738 | 1.4:+87993186 | MS.gene90810:CDS |
| AACTGAATAATCACAGTCCC+AGG | 0.545871 | 1.4:-87993426 | None:intergenic |
| TTTGGATGGATCCGAGTCGG+AGG | 0.550758 | 1.4:-87993094 | None:intergenic |
| GGAAGATACACGCCGGAACC+CGG | 0.552706 | 1.4:-87993243 | None:intergenic |
| TTCCGGCGTGTATCTTCCGC+CGG | 0.553388 | 1.4:+87993248 | MS.gene90810:CDS |
| CCGGAAACCGGTTCTTTCAG+CGG | 0.554515 | 1.4:-87993273 | None:intergenic |
| TAACAAGGCTACATCGGGGT+CGG | 0.556442 | 1.4:+87993218 | MS.gene90810:CDS |
| GCGGTCGTCGAAGTCTTCTC+CGG | 0.559155 | 1.4:-87993124 | None:intergenic |
| GGTTTCCGGTGGGTTGAAGG+TGG | 0.563703 | 1.4:+87993287 | MS.gene90810:CDS |
| TCTTGTAACGGATACGGAGT+TGG | 0.566312 | 1.4:-87993152 | None:intergenic |
| ATTAACGGCACTGTTGTTGG+TGG | 0.571400 | 1.4:+87993365 | MS.gene90810:CDS |
| GATTTGGAAGAAGAAGAAAG+TGG | 0.572368 | 1.4:-87993012 | None:intergenic |
| TTGGATGGATCCGAGTCGGA+GGG | 0.580686 | 1.4:-87993093 | None:intergenic |
| CTCCGTATCCGTTACAAGAG+AGG | 0.587354 | 1.4:+87993156 | MS.gene90810:CDS |
| GTTATCAATTATCACACACC+TGG | 0.587535 | 1.4:+87993408 | MS.gene90810:CDS |
| TGGCTCGTGTGGTGAAGAGA+AGG | 0.589646 | 1.4:-87993053 | None:intergenic |
| TCAAACACCACCCTCCGACT+CGG | 0.590606 | 1.4:+87993083 | MS.gene90810:CDS |
| GGGTGGTGTTTGAGGTTCGG+TGG | 0.598214 | 1.4:-87993073 | None:intergenic |
| GGTTCTTTCAGCGGCACCGG+CGG | 0.605564 | 1.4:-87993264 | None:intergenic |
| GATGGATCCGAGTCGGAGGG+TGG | 0.610065 | 1.4:-87993090 | None:intergenic |
| TGAAAGAACCGGTTTCCGGT+GGG | 0.610132 | 1.4:+87993277 | MS.gene90810:CDS |
| TCGTGTGGTGAAGAGAAGGA+AGG | 0.614529 | 1.4:-87993049 | None:intergenic |
| TTGAGGTTCGGTGGCTCGTG+TGG | 0.617678 | 1.4:-87993064 | None:intergenic |
| TAACTCCACCTTCAACCCAC+CGG | 0.625656 | 1.4:-87993292 | None:intergenic |
| CGTGTGGTGAAGAGAAGGAA+GGG | 0.631699 | 1.4:-87993048 | None:intergenic |
| GGGTTTGGATGGATCCGAGT+CGG | 0.641564 | 1.4:-87993097 | None:intergenic |
| CACCGGCGGAAGATACACGC+CGG | 0.671007 | 1.4:-87993250 | None:intergenic |
| TTATCAATTATCACACACCT+GGG | 0.681683 | 1.4:+87993409 | MS.gene90810:CDS |
| GGATACGGAGTTGGTTAATG+CGG | 0.688257 | 1.4:-87993143 | None:intergenic |
| AATCTAACAAGGCTACATCG+GGG | 0.786766 | 1.4:+87993214 | MS.gene90810:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GATTATTCAGTTATATTTTA+TGG | + | chr1.4:87993437-87993456 | MS.gene90810:CDS | 15.0% |
| ! | AAAAAGAGAAAATCAGTGTT+TGG | + | chr1.4:87993490-87993509 | MS.gene90810:CDS | 25.0% |
| ! | AAAAGGCAAAAAATCTAACA+AGG | + | chr1.4:87993203-87993222 | MS.gene90810:CDS | 25.0% |
| ! | AATTGCATTACTTGGATTAA+CGG | + | chr1.4:87993350-87993369 | MS.gene90810:CDS | 25.0% |
| ! | TTAGTTGAAGAAGAATTATC+AGG | - | chr1.4:87993516-87993535 | None:intergenic | 25.0% |
| !!! | TGTTAGATTTTTTGCCTTTT+CGG | - | chr1.4:87993203-87993222 | None:intergenic | 25.0% |
| AAAATCTAACAAGGCTACAT+CGG | + | chr1.4:87993212-87993231 | MS.gene90810:CDS | 30.0% | |
| AAGAAGAAGAAAGTGGAAAT+GGG | - | chr1.4:87993008-87993027 | None:intergenic | 30.0% | |
| AATGGTAGAATTGCATTACT+TGG | + | chr1.4:87993342-87993361 | MS.gene90810:CDS | 30.0% | |
| TTATCAATTATCACACACCT+GGG | + | chr1.4:87993409-87993428 | MS.gene90810:CDS | 30.0% | |
| AAATCTAACAAGGCTACATC+GGG | + | chr1.4:87993213-87993232 | MS.gene90810:CDS | 35.0% | |
| AAGAAAGCAGAAATCCGAAA+AGG | + | chr1.4:87993186-87993205 | MS.gene90810:CDS | 35.0% | |
| AGATACAGTGAGAGATTGAA+TGG | + | chr1.4:87993324-87993343 | MS.gene90810:CDS | 35.0% | |
| GAAGAAGAAGAAAGTGGAAA+TGG | - | chr1.4:87993009-87993028 | None:intergenic | 35.0% | |
| GATTTGGAAGAAGAAGAAAG+TGG | - | chr1.4:87993015-87993034 | None:intergenic | 35.0% | |
| GTTATCAATTATCACACACC+TGG | + | chr1.4:87993408-87993427 | MS.gene90810:CDS | 35.0% | |
| ! | CTTCTTCCAAATCTTTTCTC+CGG | + | chr1.4:87993022-87993041 | MS.gene90810:CDS | 35.0% |
| AACTGAATAATCACAGTCCC+AGG | - | chr1.4:87993429-87993448 | None:intergenic | 40.0% | |
| AATCTAACAAGGCTACATCG+GGG | + | chr1.4:87993214-87993233 | MS.gene90810:CDS | 40.0% | |
| ATTAACGGCACTGTTGTTGG+TGG | + | chr1.4:87993365-87993384 | MS.gene90810:CDS | 45.0% | |
| TCGGATTTCTGCTTTCTTGC+CGG | - | chr1.4:87993184-87993203 | None:intergenic | 45.0% | |
| TGGATTAACGGCACTGTTGT+TGG | + | chr1.4:87993362-87993381 | MS.gene90810:CDS | 45.0% | |
| ! | GGATACGGAGTTGGTTAATG+CGG | - | chr1.4:87993146-87993165 | None:intergenic | 45.0% |
| !! | TCTTGTAACGGATACGGAGT+TGG | - | chr1.4:87993155-87993174 | None:intergenic | 45.0% |
| !! | TGCCTCTCTTGTAACGGATA+CGG | - | chr1.4:87993161-87993180 | None:intergenic | 45.0% |
| CGTGTGGTGAAGAGAAGGAA+GGG | - | chr1.4:87993051-87993070 | None:intergenic | 50.0% | |
| CTCCGTATCCGTTACAAGAG+AGG | + | chr1.4:87993156-87993175 | MS.gene90810:CDS | 50.0% | |
| GATCCATCCAAACCCGAATC+CGG | + | chr1.4:87993105-87993124 | MS.gene90810:CDS | 50.0% | |
| GGGTCTCCGGAGAAAAGATT+TGG | - | chr1.4:87993031-87993050 | None:intergenic | 50.0% | |
| GTCGAAGTCTTCTCCGGATT+CGG | - | chr1.4:87993121-87993140 | None:intergenic | 50.0% | |
| GTGGAGCTTGCAACTGGAAA+AGG | + | chr1.4:87993384-87993403 | MS.gene90810:CDS | 50.0% | |
| TAACAAGGCTACATCGGGGT+CGG | + | chr1.4:87993218-87993237 | MS.gene90810:CDS | 50.0% | |
| TAACTCCACCTTCAACCCAC+CGG | - | chr1.4:87993295-87993314 | None:intergenic | 50.0% | |
| TCGAAGTCTTCTCCGGATTC+GGG | - | chr1.4:87993120-87993139 | None:intergenic | 50.0% | |
| TCGTGTGGTGAAGAGAAGGA+AGG | - | chr1.4:87993052-87993071 | None:intergenic | 50.0% | |
| TGAAGAGAAGGAAGGGTCTC+CGG | - | chr1.4:87993044-87993063 | None:intergenic | 50.0% | |
| ! | ATCCGTTACAAGAGAGGCAC+CGG | + | chr1.4:87993162-87993181 | MS.gene90810:CDS | 50.0% |
| ! | GTGGGTTGAAGGTGGAGTTA+GGG | + | chr1.4:87993295-87993314 | MS.gene90810:CDS | 50.0% |
| ! | TGAAAGAACCGGTTTCCGGT+GGG | + | chr1.4:87993277-87993296 | MS.gene90810:CDS | 50.0% |
| ! | TTGTTGGTGGAGCTTGCAAC+TGG | + | chr1.4:87993378-87993397 | MS.gene90810:CDS | 50.0% |
| AACAAGGCTACATCGGGGTC+GGG | + | chr1.4:87993219-87993238 | MS.gene90810:CDS | 55.0% | |
| ACCTTCAACCCACCGGAAAC+CGG | - | chr1.4:87993288-87993307 | None:intergenic | 55.0% | |
| GGGTTTGGATGGATCCGAGT+CGG | - | chr1.4:87993100-87993119 | None:intergenic | 55.0% | |
| TCAAACACCACCCTCCGACT+CGG | + | chr1.4:87993083-87993102 | MS.gene90810:CDS | 55.0% | |
| TGCCGGTGCCTCTCTTGTAA+CGG | - | chr1.4:87993167-87993186 | None:intergenic | 55.0% | |
| TGGCTCGTGTGGTGAAGAGA+AGG | - | chr1.4:87993056-87993075 | None:intergenic | 55.0% | |
| TTGGATGGATCCGAGTCGGA+GGG | - | chr1.4:87993096-87993115 | None:intergenic | 55.0% | |
| TTTGGATGGATCCGAGTCGG+AGG | - | chr1.4:87993097-87993116 | None:intergenic | 55.0% | |
| ! | CCGCTGAAAGAACCGGTTTC+CGG | + | chr1.4:87993273-87993292 | MS.gene90810:CDS | 55.0% |
| ! | CCGGAAACCGGTTCTTTCAG+CGG | - | chr1.4:87993276-87993295 | None:intergenic | 55.0% |
| ! | CTGAAAGAACCGGTTTCCGG+TGG | + | chr1.4:87993276-87993295 | MS.gene90810:CDS | 55.0% |
| ! | GGAGGGTGGTGTTTGAGGTT+CGG | - | chr1.4:87993079-87993098 | None:intergenic | 55.0% |
| ! | GTCTTCTCCGGATTCGGGTT+TGG | - | chr1.4:87993115-87993134 | None:intergenic | 55.0% |
| ! | TCTCCGGATTCGGGTTTGGA+TGG | - | chr1.4:87993111-87993130 | None:intergenic | 55.0% |
| !! | GGTGGGTTGAAGGTGGAGTT+AGG | + | chr1.4:87993294-87993313 | MS.gene90810:CDS | 55.0% |
| GAGTCGGAGGGTGGTGTTTG+AGG | - | chr1.4:87993084-87993103 | None:intergenic | 60.0% | |
| GCGGTCGTCGAAGTCTTCTC+CGG | - | chr1.4:87993127-87993146 | None:intergenic | 60.0% | |
| GGAAGATACACGCCGGAACC+CGG | - | chr1.4:87993246-87993265 | None:intergenic | 60.0% | |
| TTCCGGCGTGTATCTTCCGC+CGG | + | chr1.4:87993248-87993267 | MS.gene90810:CDS | 60.0% | |
| ! | ACCGGTTCTTTCAGCGGCAC+CGG | - | chr1.4:87993270-87993289 | None:intergenic | 60.0% |
| ! | GGGTGGTGTTTGAGGTTCGG+TGG | - | chr1.4:87993076-87993095 | None:intergenic | 60.0% |
| !! | ACCGGTTTCCGGTGGGTTGA+AGG | + | chr1.4:87993284-87993303 | MS.gene90810:CDS | 60.0% |
| !! | GGTTTCCGGTGGGTTGAAGG+TGG | + | chr1.4:87993287-87993306 | MS.gene90810:CDS | 60.0% |
| !! | TTGAGGTTCGGTGGCTCGTG+TGG | - | chr1.4:87993067-87993086 | None:intergenic | 60.0% |
| CACCGGCGGAAGATACACGC+CGG | - | chr1.4:87993253-87993272 | None:intergenic | 65.0% | |
| GATGGATCCGAGTCGGAGGG+TGG | - | chr1.4:87993093-87993112 | None:intergenic | 65.0% | |
| ! | GGTTCTTTCAGCGGCACCGG+CGG | - | chr1.4:87993267-87993286 | None:intergenic | 65.0% |
| !! | GCCGGTGCCGCTGAAAGAAC+CGG | + | chr1.4:87993266-87993285 | MS.gene90810:CDS | 65.0% |
| GCTACATCGGGGTCGGGACC+GGG | + | chr1.4:87993225-87993244 | MS.gene90810:CDS | 70.0% | |
| GGCTACATCGGGGTCGGGAC+CGG | + | chr1.4:87993224-87993243 | MS.gene90810:CDS | 70.0% | |
| TCGGGGTCGGGACCGGGTTC+CGG | + | chr1.4:87993231-87993250 | MS.gene90810:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 87992967 | 87993539 | 87992967 | ID=MS.gene90810 |
| chr1.4 | mRNA | 87992967 | 87993539 | 87992967 | ID=MS.gene90810.t1;Parent=MS.gene90810 |
| chr1.4 | exon | 87992967 | 87993539 | 87992967 | ID=MS.gene90810.t1.exon1;Parent=MS.gene90810.t1 |
| chr1.4 | CDS | 87992967 | 87993539 | 87992967 | ID=cds.MS.gene90810.t1;Parent=MS.gene90810.t1 |
| Gene Sequence |
| Protein sequence |
