AlfalfaGEDB | MsG0680032191.01

M. sativa cultivar ZhongmuNo.1 / MsG0680032191.01

KEGG: K01126
GO: GO:0006629 GO:0008081
PFAM: PF03009

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680032191.01.T01	XP_013458000.1	94.872	390	19	1	2	391	3	391	0	761
MsG0680032191.01.T02	XP_013458000.1	94.872	390	19	1	2	391	3	391	0	761

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680032191.01.T01	Q9C907	71.649	388	108	2	4	390	3	389	0	576
MsG0680032191.01.T02	Q9C907	71.649	388	108	2	4	390	3	389	0	576

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680032191.01.T01	A0A072URQ0	94.872	390	19	1	2	391	3	391	0.0	761
MsG0680032191.01.T02	A0A072URQ0	94.872	390	19	1	2	391	3	391	0.0	761

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048887.01	MsG0680032191.01	0.800729	1.257060e-48	5.896552e-46
MsG0080048896.01	MsG0680032191.01	0.819411	1.177953e-52	9.057718e-50
MsG0080049024.01	MsG0680032191.01	0.809478	1.854971e-50	1.090248e-47
MsG0180000017.01	MsG0680032191.01	0.800842	1.191887e-48	5.607134e-46
MsG0180000040.01	MsG0680032191.01	0.819884	9.187283e-53	7.158473e-50
MsG0180000490.01	MsG0680032191.01	0.810117	1.351590e-50	8.079148e-48
MsG0180000491.01	MsG0680032191.01	0.812024	5.220720e-51	3.282366e-48
MsG0180000549.01	MsG0680032191.01	0.838686	2.480950e-57	3.348298e-54
MsG0180000655.01	MsG0680032191.01	0.808755	2.650060e-50	1.527803e-47
MsG0180000976.01	MsG0680032191.01	0.800690	1.280375e-48	5.999961e-46

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680032191.01.T01	MTR_4g109600	94.872	390	19	1	2	391	3	391	0.0	761
MsG0680032191.01.T01	MTR_4g109600	86.957	207	24	1	2	208	3	206	9.76e-127	368
MsG0680032191.01.T01	MTR_4g109600	80.892	157	28	2	236	391	163	318	4.78e-83	256
MsG0680032191.01.T01	MTR_5g094230	27.011	348	209	15	44	375	37	355	5.65e-18	86.3
MsG0680032191.01.T01	MTR_5g094230	23.620	326	215	10	47	364	358	657	4.75e-17	83.6
MsG0680032191.01.T01	MTR_5g094230	27.011	348	209	15	44	375	37	355	6.35e-18	86.3
MsG0680032191.01.T01	MTR_5g094230	23.620	326	215	10	47	364	358	657	4.11e-17	83.6
MsG0680032191.01.T01	MTR_2g450160	24.771	327	203	10	47	362	516	810	8.71e-17	82.8
MsG0680032191.01.T01	MTR_2g450160	26.437	348	209	13	36	361	174	496	6.66e-14	73.9
MsG0680032191.01.T01	MTR_3g055610	24.771	327	214	11	37	355	29	331	2.36e-15	78.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680032191.01.T01	AT1G74210	71.649	388	108	2	4	390	3	389	0.0	576
MsG0680032191.01.T01	AT5G08030	74.788	353	88	1	16	368	31	382	0.0	558
MsG0680032191.01.T01	AT5G08030	74.788	353	88	1	16	368	16	367	0.0	558
MsG0680032191.01.T01	AT1G66970	23.734	316	210	9	47	355	391	682	3.27e-17	84.0
MsG0680032191.01.T01	AT1G66970	23.734	316	210	9	47	355	369	660	3.82e-17	84.0
MsG0680032191.01.T01	AT1G66970	23.175	315	213	8	47	355	175	466	4.36e-17	83.6
MsG0680032191.01.T01	AT3G20520	21.231	325	231	7	47	364	340	646	1.86e-16	81.6
MsG0680032191.01.T01	AT5G55480	21.321	333	232	9	47	372	368	677	1.93e-16	81.6
MsG0680032191.01.T01	AT4G26690	22.082	317	213	9	47	355	363	653	2.71e-16	81.3
MsG0680032191.01.T01	AT1G66980	24.051	316	208	8	47	355	401	691	2.76e-13	72.0

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Find 19 sgRNAs with CRISPR-Local

Find 217 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATAAGAAATTAGTAATGT+TGG	0.432858	6:+35890056	MsG0680032191.01.T01:three_prime_UTR
AATAGCCAATCTATATTGAT+TGG	0.386554	6:+35890089	MsG0680032191.01.T01:three_prime_UTR
ATCAACAAAATGGGAATTGA+TGG	0.404261	6:+35889736	MsG0680032191.01.T01:CDS
ATGCAGTCTTGTCATCTTGA+CGG	0.475282	6:-35889816	None:intergenic
CAATTCCAATCAATATAGAT+TGG	0.497293	6:-35890094	None:intergenic
CAGTAATCATACTCCTTGTA+AGG	0.464985	6:-35889712	None:intergenic
CTTACAAGGAGTATGATTAC+TGG	0.378731	6:+35889713	MsG0680032191.01.T01:CDS
CTTTAGTCAAGATCCTTACA+AGG	0.536799	6:+35889699	MsG0680032191.01.T01:CDS
GATTACTGGATCAACAAAAT+GGG	0.486674	6:+35889727	MsG0680032191.01.T01:CDS
GCCTGTTTACAGATTTCACT+GGG	0.542364	6:+35889758	MsG0680032191.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAACATATGAAGGGAAACA+AGG	-	Chr6:35889589-35889608	None:intergenic	30.0%
AAAAGAACATATGAAGTCGA+AGG	+	Chr6:35887869-35887888	MsG0680032191.01.T01:CDS	30.0%
AAAAGCTACTATGAGTAGAA+AGG	-	Chr6:35888438-35888457	None:intergenic	30.0%
AAAGAACATATGAAGTCGAA+GGG	+	Chr6:35887870-35887889	MsG0680032191.01.T01:CDS	30.0%
AAAGCTACTATGAGTAGAAA+GGG	-	Chr6:35888437-35888456	None:intergenic	30.0%
AAATAAATGAAGGCAAGGTA+AGG	-	Chr6:35886694-35886713	None:intergenic	30.0%
AAGCAATATGTTGTAGGAAT+TGG	+	Chr6:35889450-35889469	MsG0680032191.01.T01:CDS	30.0%
ACACAGATATAATCCAGTTA+GGG	-	Chr6:35886605-35886624	None:intergenic	30.0%
ACATATAAGAACACCATCTT+TGG	-	Chr6:35887786-35887805	None:intergenic	30.0%
ACGAGCAATAGAAAAACAAA+AGG	-	Chr6:35887360-35887379	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr6	gene	35886346	35890197	35886346	ID=MsG0680032191.01;Name=MsG0680032191.01
Chr6	mRNA	35886346	35890183	35886346	ID=MsG0680032191.01.T01;Parent=MsG0680032191.01;Name=MsG0680032191.01.T01;_AED=0.48;_eAED=0.48;_QI=137\|1\|1\|1\|0.75\|0.55\|9\|230\|391
Chr6	exon	35889652	35889940	35889652	ID=MsG0680032191.01.T01:exon:19536;Parent=MsG0680032191.01.T01
Chr6	exon	35890009	35890183	35890009	ID=MsG0680032191.01.T01:exon:19537;Parent=MsG0680032191.01.T01
Chr6	five_prime_UTR	35886346	35886482	35886346	ID=MsG0680032191.01.T01:five_prime_utr;Parent=MsG0680032191.01.T01
Chr6	CDS	35886483	35886498	35886483	ID=MsG0680032191.01.T01:cds;Parent=MsG0680032191.01.T01
Chr6	CDS	35887349	35887527	35887349	ID=MsG0680032191.01.T01:cds;Parent=MsG0680032191.01.T01
Chr6	CDS	35887734	35887917	35887734	ID=MsG0680032191.01.T01:cds;Parent=MsG0680032191.01.T01
Chr6	CDS	35888353	35888427	35888353	ID=MsG0680032191.01.T01:cds;Parent=MsG0680032191.01.T01
Chr6	CDS	35888518	35888627	35888518	ID=MsG0680032191.01.T01:cds;Parent=MsG0680032191.01.T01
Chr6	CDS	35889030	35889248	35889030	ID=MsG0680032191.01.T01:cds;Parent=MsG0680032191.01.T01
Chr6	CDS	35889408	35889566	35889408	ID=MsG0680032191.01.T01:cds;Parent=MsG0680032191.01.T01
Chr6	CDS	35889652	35889885	35889652	ID=MsG0680032191.01.T01:cds;Parent=MsG0680032191.01.T01
Chr6	three_prime_UTR	35889886	35889940	35889886	ID=MsG0680032191.01.T01:three_prime_utr;Parent=MsG0680032191.01.T01
Chr6	three_prime_UTR	35890009	35890183	35890009	ID=MsG0680032191.01.T01:three_prime_utr;Parent=MsG0680032191.01.T01
Chr6	mRNA	35886346	35890197	35886346	ID=MsG0680032191.01.T02;Parent=MsG0680032191.01;Name=MsG0680032191.01.T02;_AED=0.48;_eAED=0.48;_QI=137\|1\|1\|1\|0.85\|0.75\|8\|312\|391
Chr6	exon	35889652	35890197	35889652	ID=MsG0680032191.01.T02:exon:19538;Parent=MsG0680032191.01.T02
Chr6	five_prime_UTR	35886346	35886482	35886346	ID=MsG0680032191.01.T02:five_prime_utr;Parent=MsG0680032191.01.T02
Chr6	CDS	35886483	35886498	35886483	ID=MsG0680032191.01.T02:cds;Parent=MsG0680032191.01.T02
Chr6	CDS	35887349	35887527	35887349	ID=MsG0680032191.01.T02:cds;Parent=MsG0680032191.01.T02
Chr6	CDS	35887734	35887917	35887734	ID=MsG0680032191.01.T02:cds;Parent=MsG0680032191.01.T02
Chr6	CDS	35888353	35888427	35888353	ID=MsG0680032191.01.T02:cds;Parent=MsG0680032191.01.T02
Chr6	CDS	35888518	35888627	35888518	ID=MsG0680032191.01.T02:cds;Parent=MsG0680032191.01.T02
Chr6	CDS	35889030	35889248	35889030	ID=MsG0680032191.01.T02:cds;Parent=MsG0680032191.01.T02
Chr6	CDS	35889408	35889566	35889408	ID=MsG0680032191.01.T02:cds;Parent=MsG0680032191.01.T02
Chr6	CDS	35889652	35889885	35889652	ID=MsG0680032191.01.T02:cds;Parent=MsG0680032191.01.T02
Chr6	three_prime_UTR	35889886	35890197	35889886	ID=MsG0680032191.01.T02:three_prime_utr;Parent=MsG0680032191.01.T02

Gene Sequence

>MsG0680032191.01.T02

ATGTCTTCGTTATCGGGTTTTGCACCTTTTGTTTTTCTATTGCTCGTGATTGGGGGCACGGCTAGGCCTTTGTATCCACTTCCTGGCAAAGGTGACCATGGAAGTAAGCAGCCTCTTCAAACTTTTCGCCCATATAACGTTGCGCATAGGGGCTCAAATGGAGAACTTCCTGAAGAAACTCGCGAGGCATATCTGAGAGCTATTGAGGAAGGTGCAGACTTCATTGAAACAGATATATTATCTTCCAAAGATGGTGTTCTTATATGTTTCCACGACTTTATTCTTGATAATACTACTGACATAGCAAAATACAAAGAGTTTGCTAATCGTAAAAGAACATATGAAGTCGAAGGGGCCAATCACACTGGATTTTTTGTCGTTGATTTCACATTAAAGGAATTAAAGTCGCTGAGGGTGAATCAGAGGTTCAACTTTAGGGATCCACAGTTTAATGGAAAATTTCAAATAATCACTTTTGAAGAGTTCATTACCATAGCATTGGATGCACCCAGAGTTGTTGGAATATATCCTGAGATAAAAAATCCAGTACTTATCAATCAACATGTGAAATGGTCGGACGGCAAACGATTTGAGGACAGGTTTGTTGAAACACTTCGGAAATATGGATACAAGGGTTCATACCTGTCAAAACATTGGTTGAAACAACCTGCATTTATACAGTCATTTGCTCCAACTTCACTTGTGTATATATCAAATCAAACGGACTTGCCTAAGATTTTTTTAATCGATGATGTTCATTTTTCAACACAAGACACTAATCAGTCATATTGGGAAATTACATCTGATACATACCTAGACTACATAAAGCAATATGTTGTAGGAATTGGACCTTCGAAGGATACTATACTAGTACCTCTGGTTAACAAGTATGTGATGACTCCTTCGGATCTTGTTGCCAGGGCACATGCTCATAATCTGCAGGTGCATCCATACACGTACAGAAATGAGAACCACTATTTGCATTTCAACTTTAGTCAAGATCCTTACAAGGAGTATGATTACTGGATCAACAAAATGGGAATTGATGGCCTGTTTACAGATTTCACTGGGAGTCTTCATAATTTTCAGGAATGGACAAACCCTAACCGTCAAGATGACAAGACTGCATCCGAGCTATTACATAAAATTGCTGTCTTAGCATCCGCTTATGAATGA

>MsG0680032191.01.T01

Protein sequence

>MsG0680032191.01.T02

MSSLSGFAPFVFLLLVIGGTARPLYPLPGKGDHGSKQPLQTFRPYNVAHRGSNGELPEETREAYLRAIEEGADFIETDILSSKDGVLICFHDFILDNTTDIAKYKEFANRKRTYEVEGANHTGFFVVDFTLKELKSLRVNQRFNFRDPQFNGKFQIITFEEFITIALDAPRVVGIYPEIKNPVLINQHVKWSDGKRFEDRFVETLRKYGYKGSYLSKHWLKQPAFIQSFAPTSLVYISNQTDLPKIFLIDDVHFSTQDTNQSYWEITSDTYLDYIKQYVVGIGPSKDTILVPLVNKYVMTPSDLVARAHAHNLQVHPYTYRNENHYLHFNFSQDPYKEYDYWINKMGIDGLFTDFTGSLHNFQEWTNPNRQDDKTASELLHKIAVLASAYE*

>MsG0680032191.01.T01