AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480020363.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020363.01.T01 MTR_4g049340 99.153 118 1 0 1 118 122 239 3.02e-84 246
MsG0480020363.01.T01 MTR_7g085070 63.025 119 43 1 1 118 128 246 7.33e-51 161
MsG0480020363.01.T01 MTR_6g033010 55.357 112 50 0 1 112 127 238 6.66e-45 146
MsG0480020363.01.T01 MTR_6g033010 57.534 73 31 0 1 73 127 199 5.46e-26 96.7
MsG0480020363.01.T01 MTR_6g033010 58.824 68 28 0 1 68 127 194 4.98e-25 94.7
MsG0480020363.01.T01 MTR_7g088310 40.171 117 68 2 3 118 117 232 2.57e-20 82.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020363.01.T01 AT3G04090 55.046 109 49 0 1 109 119 227 2.46e-41 137
MsG0480020363.01.T01 AT5G18290 47.458 118 58 1 5 118 126 243 4.04e-34 118
MsG0480020363.01.T01 AT5G18290 47.458 118 58 1 5 118 126 243 4.04e-34 118
MsG0480020363.01.T01 AT3G56950 38.983 118 66 2 3 117 116 230 1.68e-19 80.5
MsG0480020363.01.T01 AT3G56950 38.983 118 66 2 3 117 116 230 2.43e-19 80.5

Find 27 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTCTTCTTGTTATGCTTA+AGG 0.242398 4:-39823470 MsG0480020363.01.T01:CDS
TTCTGTGGCAACTTTGGTTT+TGG 0.253700 4:-39823413 MsG0480020363.01.T01:CDS
ATTCTTCTTGTTATGCTTAA+GGG 0.255110 4:-39823469 MsG0480020363.01.T01:CDS
ATTATAGGTTCTGGTTATAC+TGG 0.294451 4:-39823388 MsG0480020363.01.T01:CDS
CCCTGCTAATGCATTTGGTT+GGG 0.316346 4:-39823353 MsG0480020363.01.T01:CDS
TCTATTTATGTCACCGGTTA+AGG 0.319094 4:-39823233 MsG0480020363.01.T01:CDS
ATGAACCCTGCTAATGCATT+TGG 0.350303 4:-39823358 MsG0480020363.01.T01:CDS
CAAGCTTTCTTCTCCTTAAC+CGG 0.351841 4:+39823220 None:intergenic
TTGAAAGTTGATTTGCATAC+AGG 0.359134 4:-39823535 MsG0480020363.01.T01:CDS
TTTGGATATGTCCTTTACTT+GGG 0.390759 4:-39823285 MsG0480020363.01.T01:CDS
TTTGCTTTCTGTGGCAACTT+TGG 0.391117 4:-39823419 MsG0480020363.01.T01:CDS
TGGGGCTAGTTCTGCTGCTA+TGG 0.418523 4:-39823266 MsG0480020363.01.T01:CDS
GCCCAACCAAATGCATTAGC+AGG 0.448752 4:+39823352 None:intergenic
GCATTAGCAGGGTTCATAGA+AGG 0.483858 4:+39823364 None:intergenic
GCAGAACTAGCCCCAAGTAA+AGG 0.494786 4:+39823274 None:intergenic
TTGGATATGTCCTTTACTTG+GGG 0.500215 4:-39823284 MsG0480020363.01.T01:CDS
TGCAAATCAACTTTCAAAGA+AGG 0.500886 4:+39823541 None:intergenic
TGAACAATAAGCATAATACT+TGG 0.504932 4:-39823324 MsG0480020363.01.T01:CDS
ACCCTGCTAATGCATTTGGT+TGG 0.505171 4:-39823354 MsG0480020363.01.T01:CDS
CATACAGGTGCTATTGCTGA+AGG 0.523194 4:-39823520 MsG0480020363.01.T01:CDS
GAAGGTTTATTTGCTTTCTG+TGG 0.527749 4:-39823428 MsG0480020363.01.T01:CDS
TACAGGTGCTATTGCTGAAG+GGG 0.547592 4:-39823518 MsG0480020363.01.T01:CDS
AGCAAATAAACCTTCAACCA+AGG 0.552634 4:+39823436 None:intergenic
ATACAGGTGCTATTGCTGAA+GGG 0.571193 4:-39823519 MsG0480020363.01.T01:CDS
GAACAATAAGCATAATACTT+GGG 0.590254 4:-39823323 MsG0480020363.01.T01:CDS
CCCAACCAAATGCATTAGCA+GGG 0.593803 4:+39823353 None:intergenic
GAATACAAAGACATGGTGAA+AGG 0.602834 4:-39823565 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GAACAATAAGCATAATACTT+GGG - Chr4:39823449-39823468 MsG0480020363.01.T01:CDS 25.0%
! TGAACAATAAGCATAATACT+TGG - Chr4:39823448-39823467 MsG0480020363.01.T01:CDS 25.0%
!! ATTCTTCTTGTTATGCTTAA+GGG - Chr4:39823303-39823322 MsG0480020363.01.T01:CDS 25.0%
!! TATTCTTCTTGTTATGCTTA+AGG - Chr4:39823302-39823321 MsG0480020363.01.T01:CDS 25.0%
!!! ACTTTGGTTTTGGTTATTAT+AGG - Chr4:39823369-39823388 MsG0480020363.01.T01:CDS 25.0%
TGCAAATCAACTTTCAAAGA+AGG + Chr4:39823234-39823253 None:intergenic 30.0%
TTTGGATATGTCCTTTACTT+GGG - Chr4:39823487-39823506 MsG0480020363.01.T01:CDS 30.0%
! ATTATAGGTTCTGGTTATAC+TGG - Chr4:39823384-39823403 MsG0480020363.01.T01:CDS 30.0%
! GGGAGCAATTTTATGTTTTT+TGG - Chr4:39823469-39823488 MsG0480020363.01.T01:CDS 30.0%
! TCGTTTTCTATTTATGTCAC+CGG - Chr4:39823533-39823552 MsG0480020363.01.T01:CDS 30.0%
! TTTTGGATATGTCCTTTACT+TGG - Chr4:39823486-39823505 MsG0480020363.01.T01:CDS 30.0%
!! TTGAAAGTTGATTTGCATAC+AGG - Chr4:39823237-39823256 MsG0480020363.01.T01:CDS 30.0%
!!! GTTTTGGTTATTATAGGTTC+TGG - Chr4:39823375-39823394 MsG0480020363.01.T01:CDS 30.0%
AGCAAATAAACCTTCAACCA+AGG + Chr4:39823339-39823358 None:intergenic 35.0%
GAAGGTTTATTTGCTTTCTG+TGG - Chr4:39823344-39823363 MsG0480020363.01.T01:CDS 35.0%
TCCTAAAAATCCTTGGTTGA+AGG - Chr4:39823326-39823345 MsG0480020363.01.T01:CDS 35.0%
TTAAGGGTCCTAAAAATCCT+TGG - Chr4:39823319-39823338 MsG0480020363.01.T01:CDS 35.0%
TTGGATATGTCCTTTACTTG+GGG - Chr4:39823488-39823507 MsG0480020363.01.T01:CDS 35.0%
! ACCTTCAACCAAGGATTTTT+AGG + Chr4:39823330-39823349 None:intergenic 35.0%
! TCTATTTATGTCACCGGTTA+AGG - Chr4:39823539-39823558 MsG0480020363.01.T01:CDS 35.0%
ATGAACCCTGCTAATGCATT+TGG - Chr4:39823414-39823433 MsG0480020363.01.T01:CDS 40.0%
CAAGCTTTCTTCTCCTTAAC+CGG + Chr4:39823555-39823574 None:intergenic 40.0%
TTTGCTTTCTGTGGCAACTT+TGG - Chr4:39823353-39823372 MsG0480020363.01.T01:CDS 40.0%
!! ATACAGGTGCTATTGCTGAA+GGG - Chr4:39823253-39823272 MsG0480020363.01.T01:CDS 40.0%
!! TTCTGTGGCAACTTTGGTTT+TGG - Chr4:39823359-39823378 MsG0480020363.01.T01:CDS 40.0%
ACCCTGCTAATGCATTTGGT+TGG - Chr4:39823418-39823437 MsG0480020363.01.T01:CDS 45.0%
CCCAACCAAATGCATTAGCA+GGG + Chr4:39823422-39823441 None:intergenic 45.0%
CCCTGCTAATGCATTTGGTT+GGG - Chr4:39823419-39823438 MsG0480020363.01.T01:CDS 45.0%
GCATTAGCAGGGTTCATAGA+AGG + Chr4:39823411-39823430 None:intergenic 45.0%
!! CATACAGGTGCTATTGCTGA+AGG - Chr4:39823252-39823271 MsG0480020363.01.T01:CDS 45.0%
!! TACAGGTGCTATTGCTGAAG+GGG - Chr4:39823254-39823273 MsG0480020363.01.T01:CDS 45.0%
GCAGAACTAGCCCCAAGTAA+AGG + Chr4:39823501-39823520 None:intergenic 50.0%
GCCCAACCAAATGCATTAGC+AGG + Chr4:39823423-39823442 None:intergenic 50.0%
!! TGGGGCTAGTTCTGCTGCTA+TGG - Chr4:39823506-39823525 MsG0480020363.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr4 gene 39823219 39823575 39823219 ID=MsG0480020363.01;Name=MsG0480020363.01
Chr4 mRNA 39823219 39823575 39823219 ID=MsG0480020363.01.T01;Parent=MsG0480020363.01;Name=MsG0480020363.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|118
Chr4 exon 39823219 39823575 39823219 ID=MsG0480020363.01.T01:exon:4832;Parent=MsG0480020363.01.T01
Chr4 CDS 39823219 39823575 39823219 ID=MsG0480020363.01.T01:cds;Parent=MsG0480020363.01.T01
Gene Sequence

>MsG0480020363.01.T01

ATGGTGAAAGGTCCTTCTTTGAAAGTTGATTTGCATACAGGTGCTATTGCTGAAGGGGTTTTGACTTTTACTCATAACTTTGTTATTCTTCTTGTTATGCTTAAGGGTCCTAAAAATCCTTGGTTGAAGGTTTATTTGCTTTCTGTGGCAACTTTGGTTTTGGTTATTATAGGTTCTGGTTATACTGGACCTTCTATGAACCCTGCTAATGCATTTGGTTGGGCTTATATGAACAATAAGCATAATACTTGGGAGCAATTTTATGTTTTTTGGATATGTCCTTTACTTGGGGCTAGTTCTGCTGCTATGGTTTATCGTTTTCTATTTATGTCACCGGTTAAGGAGAAGAAAGCTTGA

Protein sequence

>MsG0480020363.01.T01

MVKGPSLKVDLHTGAIAEGVLTFTHNFVILLVMLKGPKNPWLKVYLLSVATLVLVIIGSGYTGPSMNPANAFGWAYMNNKHNTWEQFYVFWICPLLGASSAAMVYRFLFMSPVKEKKA*