Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0555960 | AES90003.1 | 98.824 | 85 | 1 | 0 | 1 | 85 | 1 | 85 | 2.06e-53 | 171 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0555960 | G7JHI6 | 98.824 | 85 | 1 | 0 | 1 | 85 | 1 | 85 | 9.85e-54 | 171 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0008850 | Msa0555960 | 0.804738 | 1.870023e-49 | -8.615850e-47 |
| Msa0018470 | Msa0555960 | 0.801777 | 7.671249e-49 | -8.615850e-47 |
| Msa0047470 | Msa0555960 | 0.805785 | 1.128597e-49 | -8.615850e-47 |
| Msa0068410 | Msa0555960 | 0.800929 | 1.144470e-48 | -8.615850e-47 |
| Msa0096020 | Msa0555960 | 0.818207 | 2.211134e-52 | -8.615850e-47 |
| Msa0103060 | Msa0555960 | 0.810060 | 1.390788e-50 | -8.615850e-47 |
| Msa0158610 | Msa0555960 | 0.809396 | 1.932148e-50 | -8.615850e-47 |
| Msa0197730 | Msa0555960 | 0.823207 | 1.568585e-53 | -8.615850e-47 |
| Msa0198440 | Msa0555960 | 0.808925 | 2.437456e-50 | -8.615850e-47 |
| Msa0210880 | Msa0555960 | 0.825589 | 4.316762e-54 | -8.615850e-47 |
| Msa0216220 | Msa0555960 | 0.807844 | 4.143928e-50 | -8.615850e-47 |
| Msa0224370 | Msa0555960 | 0.818563 | 1.836549e-52 | -8.615850e-47 |
| Msa0224400 | Msa0555960 | 0.833288 | 5.813581e-56 | -8.615850e-47 |
| Msa0225880 | Msa0555960 | 0.818165 | 2.260385e-52 | -8.615850e-47 |
| Msa0230980 | Msa0555960 | 0.804974 | 1.669623e-49 | -8.615850e-47 |
| Msa0377280 | Msa0555960 | 0.802281 | 6.044184e-49 | -8.615850e-47 |
| Msa0378700 | Msa0555960 | 0.805022 | 1.631273e-49 | -8.615850e-47 |
| Msa0401340 | Msa0555960 | 0.821250 | 4.460730e-53 | -8.615850e-47 |
| Msa0404690 | Msa0555960 | 0.804435 | 2.163535e-49 | -8.615850e-47 |
| Msa0416540 | Msa0555960 | 0.809183 | 2.146458e-50 | -8.615850e-47 |
| Msa0448120 | Msa0555960 | 0.803300 | 3.722549e-49 | -8.615850e-47 |
| Msa0456480 | Msa0555960 | 0.817039 | 4.054219e-52 | -8.615850e-47 |
| Msa0467800 | Msa0555960 | 0.803546 | 3.310811e-49 | -8.615850e-47 |
| Msa0481050 | Msa0555960 | 0.812123 | 4.966100e-51 | -8.615850e-47 |
| Msa0243470 | Msa0555960 | 0.801926 | 7.149668e-49 | -8.615850e-47 |
| Msa0253160 | Msa0555960 | 0.804595 | 2.002915e-49 | -8.615850e-47 |
| Msa0260180 | Msa0555960 | 0.804018 | 2.641915e-49 | -8.615850e-47 |
| Msa0260470 | Msa0555960 | 0.815754 | 7.860910e-52 | -8.615850e-47 |
| Msa0265210 | Msa0555960 | 0.817392 | 3.376779e-52 | -8.615850e-47 |
| Msa0265580 | Msa0555960 | 0.827850 | 1.246015e-54 | -8.615850e-47 |
| Msa0270460 | Msa0555960 | 0.812388 | 4.347339e-51 | -8.615850e-47 |
| Msa0278670 | Msa0555960 | 0.807848 | 4.136643e-50 | -8.615850e-47 |
| Msa0295570 | Msa0555960 | 0.804054 | 2.597362e-49 | -8.615850e-47 |
| Msa0303200 | Msa0555960 | 0.813630 | 2.322246e-51 | -8.615850e-47 |
| Msa0303440 | Msa0555960 | 0.833574 | 4.931937e-56 | -8.615850e-47 |
| Msa0303500 | Msa0555960 | 0.840815 | 6.927532e-58 | -8.615850e-47 |
| Msa0304110 | Msa0555960 | 0.823064 | 1.693954e-53 | -8.615850e-47 |
| Msa0318070 | Msa0555960 | 0.814785 | 1.291162e-51 | -8.615850e-47 |
| Msa0336270 | Msa0555960 | 0.813979 | 1.945879e-51 | -8.615850e-47 |
| Msa0341190 | Msa0555960 | 0.816088 | 6.623458e-52 | -8.615850e-47 |
| Msa0362430 | Msa0555960 | 0.812164 | 4.865771e-51 | -8.615850e-47 |
| Msa0500490 | Msa0555960 | 0.816446 | 5.506303e-52 | -8.615850e-47 |
| Msa0514260 | Msa0555960 | 0.800020 | 1.753288e-48 | -8.615850e-47 |
| Msa0521940 | Msa0555960 | -0.809218 | 2.109819e-50 | -8.615850e-47 |
| Msa0545230 | Msa0555960 | 0.802321 | 5.930711e-49 | -8.615850e-47 |
| Msa0555960 | Msa0568610 | 0.814456 | 1.526392e-51 | -8.615850e-47 |
| Msa0555960 | Msa0579340 | 0.812840 | 3.461594e-51 | -8.615850e-47 |
| Msa0555960 | Msa0580540 | 0.845660 | 3.535681e-59 | -8.615850e-47 |
| Msa0555960 | Msa0601920 | -0.802890 | 4.525042e-49 | -8.615850e-47 |
| Msa0555960 | Msa0610360 | 0.994491 | 1.087086e-207 | -8.615850e-47 |
| Msa0555960 | Msa0617120 | 0.820707 | 5.949127e-53 | -8.615850e-47 |
| Msa0555960 | Msa0618520 | 0.810297 | 1.236610e-50 | -8.615850e-47 |
| Msa0555960 | Msa0621780 | 0.849116 | 3.974682e-60 | -8.615850e-47 |
| Msa0555960 | Msa0643020 | -0.817977 | 2.492750e-52 | -8.615850e-47 |
| Msa0555960 | Msa0650650 | 0.819359 | 1.210369e-52 | -8.615850e-47 |
| Msa0555960 | Msa0650740 | 0.816910 | 4.335135e-52 | -8.615850e-47 |
| Msa0555960 | Msa0650890 | 0.990523 | 4.845365e-183 | -8.615850e-47 |
| Msa0555960 | Msa0682730 | -0.816222 | 6.182232e-52 | -8.615850e-47 |
| Msa0555960 | Msa0713210 | 0.800068 | 1.713683e-48 | -8.615850e-47 |
| Msa0555960 | Msa0713220 | 0.800650 | 1.304421e-48 | -8.615850e-47 |
| Msa0555960 | Msa0715510 | -0.825409 | 4.763066e-54 | -8.615850e-47 |
| Msa0555960 | Msa0716600 | -0.801004 | 1.104756e-48 | -8.615850e-47 |
| Msa0555960 | Msa0754350 | 0.833501 | 5.144176e-56 | -8.615850e-47 |
| Msa0555960 | Msa0757990 | -0.801350 | 9.385770e-49 | -8.615850e-47 |
| Msa0555960 | Msa0773260 | 0.808412 | 3.137154e-50 | -8.615850e-47 |
| Msa0555960 | Msa0793060 | 0.801985 | 6.954083e-49 | -8.615850e-47 |
| Msa0555960 | Msa0793100 | 0.807855 | 4.122081e-50 | -8.615850e-47 |
| Msa0555960 | Msa0795710 | 0.848198 | 7.142083e-60 | -8.615850e-47 |
| Msa0555960 | Msa0797000 | 0.803499 | 3.386098e-49 | -8.615850e-47 |
| Msa0555960 | Msa0822370 | 0.836132 | 1.119542e-56 | -8.615850e-47 |
| Msa0555960 | Msa0833450 | 0.840150 | 1.033956e-57 | -8.615850e-47 |
| Msa0555960 | Msa0861800 | 0.824625 | 7.292330e-54 | -8.615850e-47 |
| Msa0555960 | Msa0899700 | 0.800060 | 1.720146e-48 | -8.615850e-47 |
| Msa0555960 | Msa0899730 | 0.822836 | 1.914396e-53 | -8.615850e-47 |
| Msa0555960 | Msa0930950 | 0.811141 | 8.119870e-51 | -8.615850e-47 |
| Msa0555960 | Msa0964650 | 0.817640 | 2.970126e-52 | -8.615850e-47 |
| Msa0555960 | Msa1010470 | 0.809964 | 1.458208e-50 | -8.615850e-47 |
| Msa0555960 | Msa1036020 | -0.811381 | 7.203438e-51 | -8.615850e-47 |
| Msa0555960 | Msa1040730 | -0.819235 | 1.291771e-52 | -8.615850e-47 |
| Msa0555960 | Msa1053690 | 0.822429 | 2.379906e-53 | -8.615850e-47 |
| Msa0555960 | Msa1067910 | 0.811151 | 8.080040e-51 | -8.615850e-47 |
| Msa0555960 | Msa1102200 | 0.812624 | 3.861265e-51 | -8.615850e-47 |
| Msa0555960 | Msa1122720 | 0.832866 | 7.402724e-56 | -8.615850e-47 |
| Msa0555960 | Msa1134560 | 0.810881 | 9.243246e-51 | -8.615850e-47 |
| Msa0555960 | Msa1138600 | 0.805245 | 1.465459e-49 | -8.615850e-47 |
| Msa0555960 | Msa1209790 | 0.809507 | 1.828836e-50 | -8.615850e-47 |
| Msa0555960 | Msa1209800 | 0.807929 | 3.975202e-50 | -8.615850e-47 |
| Msa0555960 | Msa1211260 | 0.829623 | 4.640586e-55 | -8.615850e-47 |
| Msa0555960 | Msa1211300 | 0.823978 | 1.035033e-53 | -8.615850e-47 |
| Msa0555960 | Msa1211390 | 0.860553 | 1.906510e-63 | -8.615850e-47 |
| Msa0555960 | Msa1219780 | -0.830251 | 3.262346e-55 | -8.615850e-47 |
| Msa0555960 | Msa1222030 | -0.806628 | 7.500429e-50 | -8.615850e-47 |
| Msa0555960 | Msa1266710 | -0.843812 | 1.112929e-58 | -8.615850e-47 |
| Msa0555960 | Msa1282210 | 0.805951 | 1.041605e-49 | -8.615850e-47 |
| Msa0555960 | Msa1299370 | 0.820190 | 7.817748e-53 | -8.615850e-47 |
| Msa0555960 | Msa1306200 | 0.814981 | 1.167839e-51 | -8.615850e-47 |
| Msa0555960 | Msa1338590 | 0.836946 | 6.945306e-57 | -8.615850e-47 |
| Msa0555960 | Msa1342400 | 0.816517 | 5.307935e-52 | -8.615850e-47 |
| Msa0555960 | Msa1349810 | 0.836249 | 1.045131e-56 | -8.615850e-47 |
| Msa0555960 | Msa1349850 | 0.809889 | 1.513774e-50 | -8.615850e-47 |
| Msa0555960 | Msa1361190 | 0.806239 | 9.060641e-50 | -8.615850e-47 |
| Msa0555960 | Msa1363690 | -0.811392 | 7.164667e-51 | -8.615850e-47 |
| Msa0555960 | Msa1370930 | -0.821765 | 3.392623e-53 | -8.615850e-47 |
| Msa0555960 | Msa1396550 | -0.824442 | 8.055996e-54 | -8.615850e-47 |
| Msa0555960 | Msa1403950 | 0.831844 | 1.325620e-55 | -8.615850e-47 |
| Msa0555960 | Msa1407210 | -0.834402 | 3.060074e-56 | -8.615850e-47 |
| Msa0555960 | Msa1413900 | 0.826165 | 3.150095e-54 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0555960 | MtrunA17_Chr4g0043181 | 98.824 | 85 | 1 | 0 | 1 | 85 | 1 | 85 | 1.90e-57 | 171 |
| Msa0555960 | MtrunA17_Chr4g0043191 | 80.899 | 89 | 13 | 1 | 1 | 85 | 1 | 89 | 1.86e-36 | 118 |
| Msa0555960 | MtrunA17_Chr8g0376661 | 76.136 | 88 | 17 | 1 | 1 | 84 | 1 | 88 | 9.45e-36 | 116 |
| Msa0555960 | MtrunA17_Chr5g0434291 | 73.034 | 89 | 20 | 1 | 1 | 85 | 1 | 89 | 3.72e-34 | 112 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0555960 | AT1G30260.1 | 57.447 | 47 | 19 | 1 | 1 | 47 | 1 | 46 | 4.53e-14 | 62.0 |
Find 14 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACAATTACCGAAGATGAT+AGG | 0.349387 | 4_1:+53969833 | Msa0555960:CDS |
| GTGTTCTGTGAACTTCCTTC+AGG | 0.374501 | 4_1:-53969957 | None:intergenic |
| GTTGATTTCCCTATCATCTT+CGG | 0.441737 | 4_1:-53969842 | None:intergenic |
| AACAATTACCGAAGATGATA+GGG | 0.480270 | 4_1:+53969834 | Msa0555960:CDS |
| AACTTCCTTCAGGAAGTCCC+TGG | 0.520126 | 4_1:-53969947 | None:intergenic |
| CCTGGTGTTAACAGTTGCAT+TGG | 0.525174 | 4_1:-53969929 | None:intergenic |
| AACAGTTGCATTGGTAGAAG+AGG | 0.536442 | 4_1:-53969920 | None:intergenic |
| CCAATGCAACTGTTAACACC+AGG | 0.562296 | 4_1:+53969929 | Msa0555960:CDS |
| TGGCAAATTCTCGCATTGCA+AGG | 0.562620 | 4_1:+53969743 | Msa0555960:CDS |
| CACAGTATGTAAGTGTGATG+AGG | 0.628878 | 4_1:+53969788 | Msa0555960:CDS |
| TAACACCAGGGACTTCCTGA+AGG | 0.629339 | 4_1:+53969942 | Msa0555960:CDS |
| CTCATCACACTTACATACTG+TGG | 0.654805 | 4_1:-53969787 | None:intergenic |
| ATCACACTTACATACTGTGG+TGG | 0.669826 | 4_1:-53969784 | None:intergenic |
| CAATGCAACTGTTAACACCA+GGG | 0.715445 | 4_1:+53969930 | Msa0555960:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GCATAGAACAACAAAAATGA+TGG | + | chr4_1:53969810-53969829 | Msa0555960:CDS | 30.0% | |
| AAACAATTACCGAAGATGAT+AGG | + | chr4_1:53969833-53969852 | Msa0555960:CDS | 30.0% | |
| AACAATTACCGAAGATGATA+GGG | + | chr4_1:53969834-53969853 | Msa0555960:CDS | 30.0% | |
| ! | GATGAAGCTAATGAACTTTT+TGG | - | chr4_1:53969892-53969911 | None:intergenic | 30.0% |
| ! | GTTGATTTCCCTATCATCTT+CGG | - | chr4_1:53969845-53969864 | None:intergenic | 35.0% |
| ! | GAAGCTAATGAACTTTTTGG+TGG | - | chr4_1:53969889-53969908 | None:intergenic | 35.0% |
| ! | TCGCATTGCAAGGTTTTTCA+TGG | + | chr4_1:53969753-53969772 | Msa0555960:CDS | 40.0% |
| ! | CATTGCAAGGTTTTTCATGG+AGG | + | chr4_1:53969756-53969775 | Msa0555960:CDS | 40.0% |
| ATCACACTTACATACTGTGG+TGG | - | chr4_1:53969787-53969806 | None:intergenic | 40.0% | |
| CTCATCACACTTACATACTG+TGG | - | chr4_1:53969790-53969809 | None:intergenic | 40.0% | |
| CACAGTATGTAAGTGTGATG+AGG | + | chr4_1:53969788-53969807 | Msa0555960:CDS | 40.0% | |
| ! | AACAGTTGCATTGGTAGAAG+AGG | - | chr4_1:53969923-53969942 | None:intergenic | 40.0% |
| CAATGCAACTGTTAACACCA+GGG | + | chr4_1:53969930-53969949 | Msa0555960:CDS | 40.0% | |
| TGGCAAATTCTCGCATTGCA+AGG | + | chr4_1:53969743-53969762 | Msa0555960:CDS | 45.0% | |
| ! | TGCAAGGTTTTTCATGGAGG+TGG | + | chr4_1:53969759-53969778 | Msa0555960:CDS | 45.0% |
| ! | CCTGGTGTTAACAGTTGCAT+TGG | - | chr4_1:53969932-53969951 | None:intergenic | 45.0% |
| CCAATGCAACTGTTAACACC+AGG | + | chr4_1:53969929-53969948 | Msa0555960:CDS | 45.0% | |
| GTGTTCTGTGAACTTCCTTC+AGG | - | chr4_1:53969960-53969979 | None:intergenic | 45.0% | |
| TAACACCAGGGACTTCCTGA+AGG | + | chr4_1:53969942-53969961 | Msa0555960:CDS | 50.0% | |
| AACTTCCTTCAGGAAGTCCC+TGG | - | chr4_1:53969950-53969969 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_1 | gene | 53969742 | 53969999 | 53969742 | ID=Msa0555960;Name=Msa0555960 |
| chr4_1 | mRNA | 53969742 | 53969999 | 53969742 | ID=Msa0555960-mRNA-1;Parent=Msa0555960;Name=Msa0555960-mRNA-1;_AED=0.29;_eAED=0.29;_QI=0|-1|0|1|-1|1|1|0|85 |
| chr4_1 | exon | 53969742 | 53969999 | 53969742 | ID=Msa0555960-mRNA-1:exon:15777;Parent=Msa0555960-mRNA-1 |
| chr4_1 | CDS | 53969742 | 53969999 | 53969742 | ID=Msa0555960-mRNA-1:cds;Parent=Msa0555960-mRNA-1 |
| Gene Sequence |
| Protein sequence |
