Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0650890 | AES90003.1 | 98.824 | 85 | 1 | 0 | 1 | 85 | 1 | 85 | 2.06e-53 | 171 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0008850 | Msa0650890 | 0.809537 | 1.801681e-50 | -8.615850e-47 |
| Msa0018470 | Msa0650890 | 0.805273 | 1.445779e-49 | -8.615850e-47 |
| Msa0039920 | Msa0650890 | 0.802579 | 5.246497e-49 | -8.615850e-47 |
| Msa0047470 | Msa0650890 | 0.817675 | 2.915555e-52 | -8.615850e-47 |
| Msa0053960 | Msa0650890 | 0.806245 | 9.034037e-50 | -8.615850e-47 |
| Msa0064550 | Msa0650890 | 0.801593 | 8.369746e-49 | -8.615850e-47 |
| Msa0068410 | Msa0650890 | 0.808796 | 2.596540e-50 | -8.615850e-47 |
| Msa0087780 | Msa0650890 | 0.800083 | 1.702186e-48 | -8.615850e-47 |
| Msa0096020 | Msa0650890 | 0.831001 | 2.137614e-55 | -8.615850e-47 |
| Msa0100250 | Msa0650890 | 0.803386 | 3.574178e-49 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0650890 | MtrunA17_Chr4g0043181 | 98.824 | 85 | 1 | 0 | 1 | 85 | 1 | 85 | 1.90e-57 | 171 |
| Msa0650890 | MtrunA17_Chr4g0043191 | 80.899 | 89 | 13 | 1 | 1 | 85 | 1 | 89 | 1.86e-36 | 118 |
| Msa0650890 | MtrunA17_Chr8g0376661 | 76.136 | 88 | 17 | 1 | 1 | 84 | 1 | 88 | 9.45e-36 | 116 |
| Msa0650890 | MtrunA17_Chr5g0434291 | 73.034 | 89 | 20 | 1 | 1 | 85 | 1 | 89 | 3.72e-34 | 112 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0650890 | AT1G30260.1 | 57.447 | 47 | 19 | 1 | 1 | 47 | 1 | 46 | 4.53e-14 | 62.0 |
Find 14 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACAATTACCGAAGATGAT+AGG | 0.349387 | 4_3:-58169259 | Msa0650890:CDS |
| AACAATTACCGAAGATGATA+GGG | 0.480270 | 4_3:-58169258 | Msa0650890:CDS |
| AACAGTTGCATTGGTAGAAG+AGG | 0.536442 | 4_3:+58169172 | None:intergenic |
| AACTTCCTTCAGGAAGTCCC+TGG | 0.520126 | 4_3:+58169145 | None:intergenic |
| ATCACACTTACATACTGTGG+TGG | 0.669826 | 4_3:+58169308 | None:intergenic |
| CAATGCAACTGTTAACACCA+GGG | 0.715445 | 4_3:-58169162 | Msa0650890:CDS |
| CACAGTATGTAAGTGTGATG+AGG | 0.628878 | 4_3:-58169304 | Msa0650890:CDS |
| CCAATGCAACTGTTAACACC+AGG | 0.562296 | 4_3:-58169163 | Msa0650890:CDS |
| CCTGGTGTTAACAGTTGCAT+TGG | 0.525174 | 4_3:+58169163 | None:intergenic |
| CTCATCACACTTACATACTG+TGG | 0.654805 | 4_3:+58169305 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GCATAGAACAACAAAAATGA+TGG | - | chr4_3:58169183-58169202 | Msa0650890:CDS | 30.0% | |
| AAACAATTACCGAAGATGAT+AGG | - | chr4_3:58169206-58169225 | Msa0650890:CDS | 30.0% | |
| AACAATTACCGAAGATGATA+GGG | - | chr4_3:58169207-58169226 | Msa0650890:CDS | 30.0% | |
| ATCACACTTACATACTGTGG+TGG | + | chr4_3:58169160-58169179 | None:intergenic | 40.0% | |
| CTCATCACACTTACATACTG+TGG | + | chr4_3:58169163-58169182 | None:intergenic | 40.0% | |
| CACAGTATGTAAGTGTGATG+AGG | - | chr4_3:58169161-58169180 | Msa0650890:CDS | 40.0% | |
| CAATGCAACTGTTAACACCA+GGG | - | chr4_3:58169303-58169322 | Msa0650890:CDS | 40.0% | |
| TGGCAAATTCTCGCATTGCA+AGG | - | chr4_3:58169116-58169135 | Msa0650890:CDS | 45.0% | |
| CCAATGCAACTGTTAACACC+AGG | - | chr4_3:58169302-58169321 | Msa0650890:CDS | 45.0% | |
| GTGTTCTGTGAACTTCCTTC+AGG | + | chr4_3:58169333-58169352 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_3 | gene | 58169115 | 58169372 | 58169115 | ID=Msa0650890;Name=Msa0650890 |
| chr4_3 | mRNA | 58169115 | 58169372 | 58169115 | ID=Msa0650890-mRNA-1;Parent=Msa0650890;Name=Msa0650890-mRNA-1;_AED=0.29;_eAED=0.29;_QI=0|-1|0|1|-1|1|1|0|85 |
| chr4_3 | exon | 58169115 | 58169372 | 58169115 | ID=Msa0650890-mRNA-1:exon:15343;Parent=Msa0650890-mRNA-1 |
| chr4_3 | CDS | 58169115 | 58169372 | 58169115 | ID=Msa0650890-mRNA-1:cds;Parent=Msa0650890-mRNA-1 |
| Gene Sequence |
>Msa0650890 ATGGCAAATTCTCGCATTGCAAGGTTTTTCATGGAGGTGGCACCACCACAGTATGTAAGTGTGATGAGGCATAGAACAACAAAAATGATGGAAACAATTACCGAAGATGATAGGGAAATCAACTCAAATGATAGTGTAATTTCACCACCAAAAAGTTCATTAGCTTCATCACTTGCTGCCTCTTCTACCAATGCAACTGTTAACACCAGGGACTTCCTGAAGGAAGTTCACAGAACACTCTCAAGTTTGAATCACTGA |
| Protein sequence |
>Msa0650890 MANSRIARFFMEVAPPQYVSVMRHRTTKMMETITEDDREINSNDSVISPPKSSLASSLAASSTNATVNTRDFLKEVHRTLSSLNH* |
