Alfalfa Gene Editing Database
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
Msa0610360 | AES90003.1 | 98.824 | 85 | 1 | 0 | 1 | 85 | 1 | 85 | 2.06e-53 | 171 |
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|
Gene ID | Type | Classification |
---|
Gene ID | Type | Classification |
---|
Co-expression Network
Gene1 | Gene2 | correlation coefficient | p_value | FDR |
---|---|---|---|---|
Msa0008850 | Msa0610360 | 0.812574 | 3.959262e-51 | -8.615850e-47 |
Msa0018470 | Msa0610360 | 0.801655 | 8.126503e-49 | -8.615850e-47 |
Msa0039920 | Msa0610360 | 0.802676 | 5.009766e-49 | -8.615850e-47 |
Msa0047470 | Msa0610360 | 0.817940 | 2.541353e-52 | -8.615850e-47 |
Msa0053960 | Msa0610360 | 0.805696 | 1.178508e-49 | -8.615850e-47 |
Msa0057740 | Msa0610360 | 0.800921 | 1.148574e-48 | -8.615850e-47 |
Msa0064550 | Msa0610360 | 0.802614 | 5.159339e-49 | -8.615850e-47 |
Msa0068410 | Msa0610360 | 0.807961 | 3.913846e-50 | -8.615850e-47 |
Msa0096020 | Msa0610360 | 0.828375 | 9.310222e-55 | -8.615850e-47 |
Msa0100250 | Msa0610360 | 0.804380 | 2.220966e-49 | -8.615850e-47 |
Msa0103060 | Msa0610360 | 0.819936 | 8.938744e-53 | -8.615850e-47 |
Msa0610360 | Msa0614980 | 0.802174 | 6.358091e-49 | -8.615850e-47 |
Msa0610360 | Msa0617120 | 0.829355 | 5.392720e-55 | -8.615850e-47 |
Msa0610360 | Msa0618520 | 0.820236 | 7.631292e-53 | -8.615850e-47 |
Msa0610360 | Msa0621780 | 0.858388 | 8.526513e-63 | -8.615850e-47 |
Msa0610360 | Msa0643020 | -0.827674 | 1.373471e-54 | -8.615850e-47 |
Msa0610360 | Msa0644770 | -0.811210 | 7.843998e-51 | -8.615850e-47 |
Msa0610360 | Msa0650650 | 0.830287 | 3.197410e-55 | -8.615850e-47 |
Msa0610360 | Msa0650740 | 0.827342 | 1.649310e-54 | -8.615850e-47 |
Msa0610360 | Msa0650890 | 0.997676 | 5.629980e-247 | -8.615850e-47 |
Msa0610360 | Msa0665790 | 0.805566 | 1.254680e-49 | -8.615850e-47 |
Msa0610360 | Msa0682730 | -0.827432 | 1.569491e-54 | -8.615850e-47 |
Msa0610360 | Msa0713210 | 0.801610 | 8.300474e-49 | -8.615850e-47 |
Msa0610360 | Msa0713220 | 0.801116 | 1.048053e-48 | -8.615850e-47 |
Msa0610360 | Msa0715510 | -0.830752 | 2.460034e-55 | -8.615850e-47 |
Msa0610360 | Msa0716600 | -0.804009 | 2.653691e-49 | -8.615850e-47 |
Msa0610360 | Msa0739610 | 0.811252 | 7.682771e-51 | -8.615850e-47 |
Msa0610360 | Msa0754350 | 0.843328 | 1.499200e-58 | -8.615850e-47 |
Msa0610360 | Msa0757990 | -0.806436 | 8.235517e-50 | -8.615850e-47 |
Msa0610360 | Msa0773260 | 0.820564 | 6.417818e-53 | -8.615850e-47 |
Msa0610360 | Msa0773290 | 0.804589 | 2.008716e-49 | -8.615850e-47 |
Msa0610360 | Msa0793060 | 0.809486 | 1.848028e-50 | -8.615850e-47 |
Msa0610360 | Msa0793100 | 0.819142 | 1.356182e-52 | -8.615850e-47 |
Msa0610360 | Msa0795710 | 0.857580 | 1.482049e-62 | -8.615850e-47 |
Msa0610360 | Msa0797000 | 0.808313 | 3.292385e-50 | -8.615850e-47 |
Msa0610360 | Msa0812970 | 0.805692 | 1.180475e-49 | -8.615850e-47 |
Msa0610360 | Msa0822370 | 0.844495 | 7.297125e-59 | -8.615850e-47 |
Msa0610360 | Msa0833450 | 0.851550 | 8.249746e-61 | -8.615850e-47 |
Msa0610360 | Msa0850720 | 0.801898 | 7.244503e-49 | -8.615850e-47 |
Msa0610360 | Msa0850740 | 0.807350 | 5.275964e-50 | -8.615850e-47 |
Msa0610360 | Msa0861800 | 0.834092 | 3.660494e-56 | -8.615850e-47 |
Msa0610360 | Msa0899700 | 0.804757 | 1.852948e-49 | -8.615850e-47 |
Msa0610360 | Msa0899730 | 0.828938 | 6.806466e-55 | -8.615850e-47 |
Msa0610360 | Msa0913120 | 0.814154 | 1.780462e-51 | -8.615850e-47 |
Msa0610360 | Msa0930950 | 0.825096 | 5.648578e-54 | -8.615850e-47 |
Msa0610360 | Msa0934830 | 0.807992 | 3.854551e-50 | -8.615850e-47 |
Msa0610360 | Msa0950410 | 0.805870 | 1.083147e-49 | -8.615850e-47 |
Msa0610360 | Msa0964650 | 0.826643 | 2.423664e-54 | -8.615850e-47 |
Msa0610360 | Msa0986620 | 0.805129 | 1.549336e-49 | -8.615850e-47 |
Msa0610360 | Msa0999590 | 0.803396 | 3.556013e-49 | -8.615850e-47 |
Msa0610360 | Msa1010470 | 0.819733 | 9.947330e-53 | -8.615850e-47 |
Msa0610360 | Msa1036020 | -0.819116 | 1.374989e-52 | -8.615850e-47 |
Msa0610360 | Msa1040730 | -0.826802 | 2.221196e-54 | -8.615850e-47 |
Msa0610360 | Msa1053690 | 0.828546 | 8.463867e-55 | -8.615850e-47 |
Msa0610360 | Msa1067910 | 0.823501 | 1.338942e-53 | -8.615850e-47 |
Msa0610360 | Msa1076280 | -0.800993 | 1.110631e-48 | -8.615850e-47 |
Msa0610360 | Msa1102200 | 0.824305 | 8.673194e-54 | -8.615850e-47 |
Msa0610360 | Msa1122720 | 0.832297 | 1.024623e-55 | -8.615850e-47 |
Msa0610360 | Msa1134560 | 0.819308 | 1.243242e-52 | -8.615850e-47 |
Msa0610360 | Msa1138600 | 0.813001 | 3.191558e-51 | -8.615850e-47 |
Msa0610360 | Msa1182830 | 0.802799 | 4.725711e-49 | -8.615850e-47 |
Msa0610360 | Msa1209790 | 0.820403 | 6.988456e-53 | -8.615850e-47 |
Msa0610360 | Msa1209800 | 0.817252 | 3.630797e-52 | -8.615850e-47 |
Msa0610360 | Msa1211260 | 0.833574 | 4.930782e-56 | -8.615850e-47 |
Msa0610360 | Msa1211300 | 0.828255 | 9.952590e-55 | -8.615850e-47 |
Msa0610360 | Msa1211390 | 0.867389 | 1.418977e-65 | -8.615850e-47 |
Msa0610360 | Msa1219780 | -0.835330 | 1.786622e-56 | -8.615850e-47 |
Msa0610360 | Msa1222030 | -0.813114 | 3.015461e-51 | -8.615850e-47 |
Msa0610360 | Msa1266710 | -0.850133 | 2.068184e-60 | -8.615850e-47 |
Msa0610360 | Msa1282210 | 0.815609 | 8.470665e-52 | -8.615850e-47 |
Msa0610360 | Msa1283120 | 0.800610 | 1.329555e-48 | -8.615850e-47 |
Msa0610360 | Msa1299370 | 0.828697 | 7.783947e-55 | -8.615850e-47 |
Msa0610360 | Msa1302010 | -0.804986 | 1.659829e-49 | -8.615850e-47 |
Msa0610360 | Msa1306200 | 0.819901 | 9.103474e-53 | -8.615850e-47 |
Msa0610360 | Msa1317710 | 0.804324 | 2.281499e-49 | -8.615850e-47 |
Msa0610360 | Msa1338590 | 0.842975 | 1.862091e-58 | -8.615850e-47 |
Msa0610360 | Msa1342400 | 0.832045 | 1.182607e-55 | -8.615850e-47 |
Msa0610360 | Msa1349810 | 0.845065 | 5.122084e-59 | -8.615850e-47 |
Msa0610360 | Msa1349850 | 0.822395 | 2.424490e-53 | -8.615850e-47 |
Msa0610360 | Msa1361190 | 0.814861 | 1.241606e-51 | -8.615850e-47 |
Msa0610360 | Msa1363690 | -0.812555 | 3.997559e-51 | -8.615850e-47 |
Msa0610360 | Msa1370930 | -0.827411 | 1.588321e-54 | -8.615850e-47 |
Msa0610360 | Msa1387520 | 0.804841 | 1.779884e-49 | -8.615850e-47 |
Msa0610360 | Msa1388520 | 0.804192 | 2.431051e-49 | -8.615850e-47 |
Msa0610360 | Msa1394900 | 0.805662 | 1.198105e-49 | -8.615850e-47 |
Msa0610360 | Msa1395580 | 0.802484 | 5.488899e-49 | -8.615850e-47 |
Msa0610360 | Msa1396550 | -0.826541 | 2.562991e-54 | -8.615850e-47 |
Msa0610360 | Msa1403950 | 0.842530 | 2.443815e-58 | -8.615850e-47 |
Msa0610360 | Msa1407210 | -0.839258 | 1.764932e-57 | -8.615850e-47 |
Msa0610360 | Msa1413900 | 0.831056 | 2.072519e-55 | -8.615850e-47 |
Msa0610360 | Msa1440180 | 0.805530 | 1.277098e-49 | -8.615850e-47 |
Msa0610360 | Msa1443550 | 0.807850 | 4.131848e-50 | -8.615850e-47 |
Msa0610360 | Msa1449740 | 0.804013 | 2.648853e-49 | -8.615850e-47 |
Msa0134380 | Msa0610360 | 0.802348 | 5.855569e-49 | -8.615850e-47 |
Msa0158610 | Msa0610360 | 0.808632 | 2.815621e-50 | -8.615850e-47 |
Msa0180600 | Msa0610360 | 0.805759 | 1.143367e-49 | -8.615850e-47 |
Msa0185340 | Msa0610360 | -0.800494 | 1.403658e-48 | -8.615850e-47 |
Msa0187980 | Msa0610360 | 0.801434 | 9.019011e-49 | -8.615850e-47 |
Msa0197730 | Msa0610360 | 0.835282 | 1.837341e-56 | -8.615850e-47 |
Msa0198440 | Msa0610360 | 0.815046 | 1.129551e-51 | -8.615850e-47 |
Msa0200840 | Msa0610360 | 0.814318 | 1.637577e-51 | -8.615850e-47 |
Msa0200870 | Msa0610360 | 0.810334 | 1.214037e-50 | -8.615850e-47 |
Msa0203180 | Msa0610360 | 0.803651 | 3.148764e-49 | -8.615850e-47 |
Msa0210880 | Msa0610360 | 0.835361 | 1.755287e-56 | -8.615850e-47 |
Msa0212730 | Msa0610360 | 0.804250 | 2.363851e-49 | -8.615850e-47 |
Msa0216220 | Msa0610360 | 0.812610 | 3.887146e-51 | -8.615850e-47 |
Msa0224370 | Msa0610360 | 0.825308 | 5.032342e-54 | -8.615850e-47 |
Msa0224400 | Msa0610360 | 0.839787 | 1.285388e-57 | -8.615850e-47 |
Msa0225880 | Msa0610360 | 0.822610 | 2.160174e-53 | -8.615850e-47 |
Msa0230980 | Msa0610360 | 0.813904 | 2.020983e-51 | -8.615850e-47 |
Msa0377280 | Msa0610360 | 0.810839 | 9.438858e-51 | -8.615850e-47 |
Msa0378670 | Msa0610360 | 0.801917 | 7.179486e-49 | -8.615850e-47 |
Msa0378700 | Msa0610360 | 0.812639 | 3.832259e-51 | -8.615850e-47 |
Msa0388200 | Msa0610360 | 0.805035 | 1.620889e-49 | -8.615850e-47 |
Msa0401340 | Msa0610360 | 0.829436 | 5.152017e-55 | -8.615850e-47 |
Msa0404690 | Msa0610360 | 0.812465 | 4.183251e-51 | -8.615850e-47 |
Msa0416540 | Msa0610360 | 0.808512 | 2.986996e-50 | -8.615850e-47 |
Msa0421800 | Msa0610360 | 0.811890 | 5.582131e-51 | -8.615850e-47 |
Msa0428330 | Msa0610360 | 0.800005 | 1.765251e-48 | -8.615850e-47 |
Msa0433670 | Msa0610360 | 0.800762 | 1.237621e-48 | -8.615850e-47 |
Msa0448120 | Msa0610360 | 0.813191 | 2.900488e-51 | -8.615850e-47 |
Msa0456480 | Msa0610360 | 0.831780 | 1.374942e-55 | -8.615850e-47 |
Msa0467800 | Msa0610360 | 0.815828 | 7.568284e-52 | -8.615850e-47 |
Msa0467880 | Msa0610360 | 0.809489 | 1.844999e-50 | -8.615850e-47 |
Msa0481050 | Msa0610360 | 0.819412 | 1.177253e-52 | -8.615850e-47 |
Msa0243470 | Msa0610360 | 0.814619 | 1.404600e-51 | -8.615850e-47 |
Msa0251150 | Msa0610360 | 0.808824 | 2.561853e-50 | -8.615850e-47 |
Msa0253160 | Msa0610360 | 0.805893 | 1.071316e-49 | -8.615850e-47 |
Msa0260180 | Msa0610360 | 0.814023 | 1.903059e-51 | -8.615850e-47 |
Msa0260470 | Msa0610360 | 0.821082 | 4.878379e-53 | -8.615850e-47 |
Msa0265210 | Msa0610360 | 0.821662 | 3.583694e-53 | -8.615850e-47 |
Msa0265580 | Msa0610360 | 0.835239 | 1.884227e-56 | -8.615850e-47 |
Msa0270460 | Msa0610360 | 0.822237 | 2.637932e-53 | -8.615850e-47 |
Msa0278670 | Msa0610360 | 0.814176 | 1.760264e-51 | -8.615850e-47 |
Msa0281940 | Msa0610360 | 0.802173 | 6.360496e-49 | -8.615850e-47 |
Msa0295570 | Msa0610360 | 0.810328 | 1.217346e-50 | -8.615850e-47 |
Msa0303200 | Msa0610360 | 0.816120 | 6.513556e-52 | -8.615850e-47 |
Msa0303440 | Msa0610360 | 0.839692 | 1.360931e-57 | -8.615850e-47 |
Msa0303500 | Msa0610360 | 0.847762 | 9.420636e-60 | -8.615850e-47 |
Msa0304110 | Msa0610360 | 0.827394 | 1.602872e-54 | -8.615850e-47 |
Msa0317360 | Msa0610360 | 0.804324 | 2.281499e-49 | -8.615850e-47 |
Msa0318070 | Msa0610360 | 0.820596 | 6.308287e-53 | -8.615850e-47 |
Msa0333900 | Msa0610360 | 0.806952 | 6.407557e-50 | -8.615850e-47 |
Msa0334020 | Msa0610360 | 0.800723 | 1.260559e-48 | -8.615850e-47 |
Msa0336270 | Msa0610360 | 0.826422 | 2.736624e-54 | -8.615850e-47 |
Msa0341190 | Msa0610360 | 0.823483 | 1.351830e-53 | -8.615850e-47 |
Msa0362430 | Msa0610360 | 0.820200 | 7.777509e-53 | -8.615850e-47 |
Msa0500490 | Msa0610360 | 0.824355 | 8.443622e-54 | -8.615850e-47 |
Msa0514260 | Msa0610360 | 0.813083 | 3.062266e-51 | -8.615850e-47 |
Msa0521570 | Msa0610360 | 0.804597 | 2.001355e-49 | -8.615850e-47 |
Msa0521940 | Msa0610360 | -0.818636 | 1.767782e-52 | -8.615850e-47 |
Msa0545230 | Msa0610360 | 0.810444 | 1.149032e-50 | -8.615850e-47 |
Msa0546080 | Msa0610360 | 0.801141 | 1.035709e-48 | -8.615850e-47 |
Msa0555960 | Msa0610360 | 0.994491 | 1.087086e-207 | -8.615850e-47 |
Msa0568610 | Msa0610360 | 0.823027 | 1.727818e-53 | -8.615850e-47 |
Msa0579340 | Msa0610360 | 0.823357 | 1.446613e-53 | -8.615850e-47 |
Msa0580540 | Msa0610360 | 0.855667 | 5.409337e-62 | -8.615850e-47 |
Msa0601920 | Msa0610360 | -0.812969 | 3.244267e-51 | -8.615850e-47 |
PPI
Gene1 | Gene2 | Type |
---|
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
Msa0610360 | MtrunA17_Chr4g0043181 | 98.824 | 85 | 1 | 0 | 1 | 85 | 1 | 85 | 1.90e-57 | 171 |
Msa0610360 | MtrunA17_Chr4g0043191 | 80.899 | 89 | 13 | 1 | 1 | 85 | 1 | 89 | 1.86e-36 | 118 |
Msa0610360 | MtrunA17_Chr8g0376661 | 76.136 | 88 | 17 | 1 | 1 | 84 | 1 | 88 | 9.45e-36 | 116 |
Msa0610360 | MtrunA17_Chr5g0434291 | 73.034 | 89 | 20 | 1 | 1 | 85 | 1 | 89 | 3.72e-34 | 112 |
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
Msa0610360 | AT1G30260.1 | 57.447 | 47 | 19 | 1 | 1 | 47 | 1 | 46 | 4.53e-14 | 62.0 |
Find 14 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
sgRNA_sequence | on_target_score | Position | Region |
---|---|---|---|
AAACAATTACCGAAGATGAT+AGG | 0.349387 | 4_2:-58062663 | Msa0610360:CDS |
GTGTTCTGTGAACTTCCTTC+AGG | 0.374501 | 4_2:+58062539 | None:intergenic |
GTTGATTTCCCTATCATCTT+CGG | 0.441737 | 4_2:+58062654 | None:intergenic |
AACAATTACCGAAGATGATA+GGG | 0.480270 | 4_2:-58062662 | Msa0610360:CDS |
AACTTCCTTCAGGAAGTCCC+TGG | 0.520126 | 4_2:+58062549 | None:intergenic |
CCTGGTGTTAACAGTTGCAT+TGG | 0.525174 | 4_2:+58062567 | None:intergenic |
AACAGTTGCATTGGTAGAAG+AGG | 0.536442 | 4_2:+58062576 | None:intergenic |
CCAATGCAACTGTTAACACC+AGG | 0.562296 | 4_2:-58062567 | Msa0610360:CDS |
TGGCAAATTCTCGCATTGCA+AGG | 0.562620 | 4_2:-58062753 | Msa0610360:CDS |
CACAGTATGTAAGTGTGATG+AGG | 0.628878 | 4_2:-58062708 | Msa0610360:CDS |
TAACACCAGGGACTTCCTGA+AGG | 0.629339 | 4_2:-58062554 | Msa0610360:CDS |
CTCATCACACTTACATACTG+TGG | 0.654805 | 4_2:+58062709 | None:intergenic |
ATCACACTTACATACTGTGG+TGG | 0.669826 | 4_2:+58062712 | None:intergenic |
CAATGCAACTGTTAACACCA+GGG | 0.715445 | 4_2:-58062566 | Msa0610360:CDS |
CRISPR-GE
badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
---|---|---|---|---|---|
GCATAGAACAACAAAAATGA+TGG | - | chr4_2:58062587-58062606 | Msa0610360:CDS | 30.0% | |
AAACAATTACCGAAGATGAT+AGG | - | chr4_2:58062610-58062629 | Msa0610360:CDS | 30.0% | |
AACAATTACCGAAGATGATA+GGG | - | chr4_2:58062611-58062630 | Msa0610360:CDS | 30.0% | |
! | GATGAAGCTAATGAACTTTT+TGG | + | chr4_2:58062669-58062688 | None:intergenic | 30.0% |
! | GTTGATTTCCCTATCATCTT+CGG | + | chr4_2:58062622-58062641 | None:intergenic | 35.0% |
! | GAAGCTAATGAACTTTTTGG+TGG | + | chr4_2:58062666-58062685 | None:intergenic | 35.0% |
! | TCGCATTGCAAGGTTTTTCA+TGG | - | chr4_2:58062530-58062549 | Msa0610360:CDS | 40.0% |
! | CATTGCAAGGTTTTTCATGG+AGG | - | chr4_2:58062533-58062552 | Msa0610360:CDS | 40.0% |
ATCACACTTACATACTGTGG+TGG | + | chr4_2:58062564-58062583 | None:intergenic | 40.0% | |
CTCATCACACTTACATACTG+TGG | + | chr4_2:58062567-58062586 | None:intergenic | 40.0% | |
CACAGTATGTAAGTGTGATG+AGG | - | chr4_2:58062565-58062584 | Msa0610360:CDS | 40.0% | |
! | AACAGTTGCATTGGTAGAAG+AGG | + | chr4_2:58062700-58062719 | None:intergenic | 40.0% |
CAATGCAACTGTTAACACCA+GGG | - | chr4_2:58062707-58062726 | Msa0610360:CDS | 40.0% | |
TGGCAAATTCTCGCATTGCA+AGG | - | chr4_2:58062520-58062539 | Msa0610360:CDS | 45.0% | |
! | TGCAAGGTTTTTCATGGAGG+TGG | - | chr4_2:58062536-58062555 | Msa0610360:CDS | 45.0% |
! | CCTGGTGTTAACAGTTGCAT+TGG | + | chr4_2:58062709-58062728 | None:intergenic | 45.0% |
CCAATGCAACTGTTAACACC+AGG | - | chr4_2:58062706-58062725 | Msa0610360:CDS | 45.0% | |
GTGTTCTGTGAACTTCCTTC+AGG | + | chr4_2:58062737-58062756 | None:intergenic | 45.0% | |
TAACACCAGGGACTTCCTGA+AGG | - | chr4_2:58062719-58062738 | Msa0610360:CDS | 50.0% | |
AACTTCCTTCAGGAAGTCCC+TGG | + | chr4_2:58062727-58062746 | None:intergenic | 50.0% |
Chromosome | Type | Strat | End | Strand | Name |
---|---|---|---|---|---|
chr4_2 | gene | 58062519 | 58062776 | 58062519 | ID=Msa0610360;Name=Msa0610360 |
chr4_2 | mRNA | 58062519 | 58062776 | 58062519 | ID=Msa0610360-mRNA-1;Parent=Msa0610360;Name=Msa0610360-mRNA-1;_AED=0.29;_eAED=0.29;_QI=0|-1|0|1|-1|1|1|0|85 |
chr4_2 | exon | 58062519 | 58062776 | 58062519 | ID=Msa0610360-mRNA-1:exon:15227;Parent=Msa0610360-mRNA-1 |
chr4_2 | CDS | 58062519 | 58062776 | 58062519 | ID=Msa0610360-mRNA-1:cds;Parent=Msa0610360-mRNA-1 |
Gene Sequence |
Protein sequence |