AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0216220


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0216220 MtrunA17_Chr2g0317751 78.378 74 14 1 1 74 1 72 9.81e-38 120
Msa0216220 MtrunA17_Chr2g0317741 64.865 74 25 1 1 74 1 73 2.72e-23 84.0
Msa0216220 MtrunA17_Chr8g0385311 44.000 75 40 2 1 74 1 74 1.07e-12 57.0
Msa0216220 MtrunA17_Chr8g0337781 41.892 74 38 3 1 71 1 72 2.12e-12 56.2
Msa0216220 MtrunA17_Chr8g0337061 37.662 77 44 2 1 74 9 84 8.54e-11 52.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 24 sgRNAs with CRISPR-Local

Find 77 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCAAACACTTGTGAGAATT+TGG 0.253219 2_1:+61945321 Msa0216220:CDS
GCCACTAACTAAATGCTCTT+TGG 0.345228 2_1:-61945401 None:intergenic
CAAGCATGGATACATATATA+TGG 0.351007 2_1:+61945491 Msa0216220:three_prime_UTR
GTGCACAAGAAACTGTTAAA+TGG 0.351514 2_1:+61945450 Msa0216220:exon
ACCAAAGAGCATTTAGTTAG+TGG 0.385720 2_1:+61945400 Msa0216220:CDS
ACATTCAAGGGACCATGTTT+CGG 0.416979 2_1:+61945349 Msa0216220:CDS
TCATATTTATCATTAAGTTA+TGG 0.428066 2_1:+61944608 Msa0216220:exon
ATTTAGTTAGTGGCAGGTGC+AGG 0.446134 2_1:+61945410 Msa0216220:CDS
GCAGGGATGACTTTCGTTGT+TGG 0.447075 2_1:+61945428 Msa0216220:CDS
CAACAAAGAGAACGAGGAAG+AGG 0.464218 2_1:-61944659 None:intergenic
AGAAACTGTTAAATGGATCA+TGG 0.482667 2_1:+61945457 Msa0216220:exon
ATGGAGAAGAAATCATTAGT+TGG 0.488503 2_1:+61944627 Msa0216220:CDS
GAATTTGGCTGGTACATTCA+AGG 0.488760 2_1:+61945336 Msa0216220:CDS
AACACTTGTGAGAATTTGGC+TGG 0.492030 2_1:+61945325 Msa0216220:CDS
ATATATGTATCCATGCTTGT+TGG 0.495303 2_1:-61945487 None:intergenic
AAGAGCATTTAGTTAGTGGC+AGG 0.516402 2_1:+61945404 Msa0216220:CDS
AATTTGGCTGGTACATTCAA+GGG 0.536130 2_1:+61945337 Msa0216220:CDS
ATACGTTATGCACTATATTG+TGG 0.546559 2_1:-61945613 None:intergenic
TTAAGTGTTTGTGTCTAGTG+TGG 0.553780 2_1:-61944559 None:intergenic
CAGTTTGAGTTACCGAAACA+TGG 0.557423 2_1:-61945361 None:intergenic
TTTAGTTAGTGGCAGGTGCA+GGG 0.572800 2_1:+61945411 Msa0216220:CDS
TCTGAATGCAGAAGAAATTG+TGG 0.648620 2_1:+61945291 Msa0216220:intron
AGAAGAAATTGTGGTGACTG+AGG 0.707441 2_1:+61945300 Msa0216220:intron
TACGTGCAACAAAGAGAACG+AGG 0.716392 2_1:-61944665 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATAATAATATACTAAGAA+TGG - chr2_1:61944701-61944720 None:intergenic 10.0%
!! TATAAAAAAATAATCAAATG+TGG + chr2_1:61944973-61944992 Msa0216220:intron 10.0%
!! ATATAATAATATACTAAGAA+TGG - chr2_1:61944701-61944720 None:intergenic 10.0%
!! TATAAAAAAATAATCAAATG+TGG + chr2_1:61944973-61944992 Msa0216220:intron 10.0%
!! TCATATTTATCATTAAGTTA+TGG + chr2_1:61944608-61944627 Msa0216220:exon 15.0%
!!! TGTTTTACGTTAATTTTTTT+AGG - chr2_1:61945013-61945032 None:intergenic 15.0%
!! TAATAATACAAATTGAACTT+TGG + chr2_1:61945088-61945107 Msa0216220:intron 15.0%
!! AAAAAAATACTTTCTAATAG+TGG - chr2_1:61945142-61945161 None:intergenic 15.0%
!! TCATATTTATCATTAAGTTA+TGG + chr2_1:61944608-61944627 Msa0216220:exon 15.0%
!!! TGTTTTACGTTAATTTTTTT+AGG - chr2_1:61945013-61945032 None:intergenic 15.0%
!! TAATAATACAAATTGAACTT+TGG + chr2_1:61945088-61945107 Msa0216220:intron 15.0%
!! AAAAAAATACTTTCTAATAG+TGG - chr2_1:61945142-61945161 None:intergenic 15.0%
!!! TGTATAATTTTGATGTCTTG+TGG - chr2_1:61944853-61944872 None:intergenic 25.0%
! TACCTAAATGTTCGAAAAAT+GGG - chr2_1:61945690-61945709 None:intergenic 25.0%
!!! TGTATAATTTTGATGTCTTG+TGG - chr2_1:61944853-61944872 None:intergenic 25.0%
! TACCTAAATGTTCGAAAAAT+GGG - chr2_1:61945690-61945709 None:intergenic 25.0%
ATGGAGAAGAAATCATTAGT+TGG + chr2_1:61944627-61944646 Msa0216220:CDS 30.0%
AAATTGAACTTTGGATAGCA+TGG + chr2_1:61945097-61945116 Msa0216220:intron 30.0%
AATTGAACTTTGGATAGCAT+GGG + chr2_1:61945098-61945117 Msa0216220:intron 30.0%
AGAAACTGTTAAATGGATCA+TGG + chr2_1:61945457-61945476 Msa0216220:exon 30.0%
ATATATGTATCCATGCTTGT+TGG - chr2_1:61945490-61945509 None:intergenic 30.0%
CAAGCATGGATACATATATA+TGG + chr2_1:61945491-61945510 Msa0216220:three_prime_UTR 30.0%
ATACGTTATGCACTATATTG+TGG - chr2_1:61945616-61945635 None:intergenic 30.0%
!! ATCCCATTTTTCGAACATTT+AGG + chr2_1:61945685-61945704 Msa0216220:three_prime_UTR 30.0%
GTACCTAAATGTTCGAAAAA+TGG - chr2_1:61945691-61945710 None:intergenic 30.0%
TTTCGAACATTTAGGTACTA+TGG + chr2_1:61945693-61945712 Msa0216220:three_prime_UTR 30.0%
ATGGAGAAGAAATCATTAGT+TGG + chr2_1:61944627-61944646 Msa0216220:CDS 30.0%
AAATTGAACTTTGGATAGCA+TGG + chr2_1:61945097-61945116 Msa0216220:intron 30.0%
AATTGAACTTTGGATAGCAT+GGG + chr2_1:61945098-61945117 Msa0216220:intron 30.0%
AGAAACTGTTAAATGGATCA+TGG + chr2_1:61945457-61945476 Msa0216220:exon 30.0%
ATATATGTATCCATGCTTGT+TGG - chr2_1:61945490-61945509 None:intergenic 30.0%
CAAGCATGGATACATATATA+TGG + chr2_1:61945491-61945510 Msa0216220:three_prime_UTR 30.0%
ATACGTTATGCACTATATTG+TGG - chr2_1:61945616-61945635 None:intergenic 30.0%
!! ATCCCATTTTTCGAACATTT+AGG + chr2_1:61945685-61945704 Msa0216220:three_prime_UTR 30.0%
GTACCTAAATGTTCGAAAAA+TGG - chr2_1:61945691-61945710 None:intergenic 30.0%
TTAAGTGTTTGTGTCTAGTG+TGG - chr2_1:61944562-61944581 None:intergenic 35.0%
TCTGAATGCAGAAGAAATTG+TGG + chr2_1:61945291-61945310 Msa0216220:intron 35.0%
AATTTGGCTGGTACATTCAA+GGG + chr2_1:61945337-61945356 Msa0216220:CDS 35.0%
!! ACCAAAGAGCATTTAGTTAG+TGG + chr2_1:61945400-61945419 Msa0216220:CDS 35.0%
GTGCACAAGAAACTGTTAAA+TGG + chr2_1:61945450-61945469 Msa0216220:exon 35.0%
TTAAGTGTTTGTGTCTAGTG+TGG - chr2_1:61944562-61944581 None:intergenic 35.0%
TCTGAATGCAGAAGAAATTG+TGG + chr2_1:61945291-61945310 Msa0216220:intron 35.0%
AATTTGGCTGGTACATTCAA+GGG + chr2_1:61945337-61945356 Msa0216220:CDS 35.0%
!! ACCAAAGAGCATTTAGTTAG+TGG + chr2_1:61945400-61945419 Msa0216220:CDS 35.0%
GTGCACAAGAAACTGTTAAA+TGG + chr2_1:61945450-61945469 Msa0216220:exon 35.0%
CGCATGATGTCATTAAGACT+TGG - chr2_1:61944817-61944836 None:intergenic 40.0%
AGAAGAAATTGTGGTGACTG+AGG + chr2_1:61945300-61945319 Msa0216220:intron 40.0%
GGCAAACACTTGTGAGAATT+TGG + chr2_1:61945321-61945340 Msa0216220:CDS 40.0%
AACACTTGTGAGAATTTGGC+TGG + chr2_1:61945325-61945344 Msa0216220:CDS 40.0%
GAATTTGGCTGGTACATTCA+AGG + chr2_1:61945336-61945355 Msa0216220:CDS 40.0%
ACATTCAAGGGACCATGTTT+CGG + chr2_1:61945349-61945368 Msa0216220:CDS 40.0%
! CAGTTTGAGTTACCGAAACA+TGG - chr2_1:61945364-61945383 None:intergenic 40.0%
GCCACTAACTAAATGCTCTT+TGG - chr2_1:61945404-61945423 None:intergenic 40.0%
!! AAGAGCATTTAGTTAGTGGC+AGG + chr2_1:61945404-61945423 Msa0216220:CDS 40.0%
! TGGATTTTCTCCAACAAGCA+TGG + chr2_1:61945477-61945496 Msa0216220:three_prime_UTR 40.0%
CGCATGATGTCATTAAGACT+TGG - chr2_1:61944817-61944836 None:intergenic 40.0%
AGAAGAAATTGTGGTGACTG+AGG + chr2_1:61945300-61945319 Msa0216220:intron 40.0%
GGCAAACACTTGTGAGAATT+TGG + chr2_1:61945321-61945340 Msa0216220:CDS 40.0%
AACACTTGTGAGAATTTGGC+TGG + chr2_1:61945325-61945344 Msa0216220:CDS 40.0%
GAATTTGGCTGGTACATTCA+AGG + chr2_1:61945336-61945355 Msa0216220:CDS 40.0%
ACATTCAAGGGACCATGTTT+CGG + chr2_1:61945349-61945368 Msa0216220:CDS 40.0%
! CAGTTTGAGTTACCGAAACA+TGG - chr2_1:61945364-61945383 None:intergenic 40.0%
GCCACTAACTAAATGCTCTT+TGG - chr2_1:61945404-61945423 None:intergenic 40.0%
!! AAGAGCATTTAGTTAGTGGC+AGG + chr2_1:61945404-61945423 Msa0216220:CDS 40.0%
! TGGATTTTCTCCAACAAGCA+TGG + chr2_1:61945477-61945496 Msa0216220:three_prime_UTR 40.0%
CAACAAAGAGAACGAGGAAG+AGG - chr2_1:61944662-61944681 None:intergenic 45.0%
TACGTGCAACAAAGAGAACG+AGG - chr2_1:61944668-61944687 None:intergenic 45.0%
ATTTAGTTAGTGGCAGGTGC+AGG + chr2_1:61945410-61945429 Msa0216220:CDS 45.0%
TTTAGTTAGTGGCAGGTGCA+GGG + chr2_1:61945411-61945430 Msa0216220:CDS 45.0%
CAACAAAGAGAACGAGGAAG+AGG - chr2_1:61944662-61944681 None:intergenic 45.0%
TACGTGCAACAAAGAGAACG+AGG - chr2_1:61944668-61944687 None:intergenic 45.0%
ATTTAGTTAGTGGCAGGTGC+AGG + chr2_1:61945410-61945429 Msa0216220:CDS 45.0%
TTTAGTTAGTGGCAGGTGCA+GGG + chr2_1:61945411-61945430 Msa0216220:CDS 45.0%
!!! ACTTTAATTTTTTTTAATAA+TGG + chr2_1:61944894-61944913 Msa0216220:intron 5.0%
!!! ACTTTAATTTTTTTTAATAA+TGG + chr2_1:61944894-61944913 Msa0216220:intron 5.0%
GCAGGGATGACTTTCGTTGT+TGG + chr2_1:61945428-61945447 Msa0216220:CDS 50.0%
GCAGGGATGACTTTCGTTGT+TGG + chr2_1:61945428-61945447 Msa0216220:CDS 50.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 61944516 61945712 61944516 ID=Msa0216220;Name=Msa0216220
chr2_1 mRNA 61944516 61945712 61944516 ID=Msa0216220-mRNA-1;Parent=Msa0216220;Name=Msa0216220-mRNA-1;_AED=0.37;_eAED=0.37;_QI=111|1|1|1|0|0|2|244|74
chr2_1 exon 61944516 61944684 61944516 ID=Msa0216220-mRNA-1:exon:17110;Parent=Msa0216220-mRNA-1
chr2_1 exon 61945302 61945712 61945302 ID=Msa0216220-mRNA-1:exon:17111;Parent=Msa0216220-mRNA-1
chr2_1 five_prime_UTR 61944516 61944626 61944516 ID=Msa0216220-mRNA-1:five_prime_utr;Parent=Msa0216220-mRNA-1
chr2_1 CDS 61944627 61944684 61944627 ID=Msa0216220-mRNA-1:cds;Parent=Msa0216220-mRNA-1
chr2_1 CDS 61945302 61945468 61945302 ID=Msa0216220-mRNA-1:cds;Parent=Msa0216220-mRNA-1
chr2_1 three_prime_UTR 61945469 61945712 61945469 ID=Msa0216220-mRNA-1:three_prime_utr;Parent=Msa0216220-mRNA-1
Gene Sequence

>Msa0216220

ATGGAGAAGAAATCATTAGTTGGCATGTGCTTCCTCTTCCTCGTTCTCTTTGTTGCACAAGAAATTGTGGTGACTGAGGCAAACACTTGTGAGAATTTGGCTGGTACATTCAAGGGACCATGTTTCGGTAACTCAAACTGTGACTTTCACTGCAGAACCAAAGAGCATTTAGTTAGTGGCAGGTGCAGGGATGACTTTCGTTGTTGGTGCACAAGAAACTGTTAA

Protein sequence

>Msa0216220

MEKKSLVGMCFLFLVLFVAQEIVVTEANTCENLAGTFKGPCFGNSNCDFHCRTKEHLVSGRCRDDFRCWCTRNC*