Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0565170 | RHN42672.1 | 97.425 | 466 | 12 | 0 | 1 | 466 | 14 | 479 | 0.0 | 939 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0565170 | sp|F4KGE8|GIL1_ARATH | 29.557 | 406 | 250 | 7 | 79 | 458 | 160 | 555 | 9.17e-42 | 159 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0565170 | A0A396GNK6 | 97.425 | 466 | 12 | 0 | 1 | 466 | 14 | 479 | 0.0 | 939 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0001960 | Msa0565170 | 0.800587 | 1.344055e-48 | -8.615850e-47 |
| Msa0010110 | Msa0565170 | 0.819085 | 1.397664e-52 | -8.615850e-47 |
| Msa0030160 | Msa0565170 | 0.810944 | 8.959785e-51 | -8.615850e-47 |
| Msa0035120 | Msa0565170 | 0.802828 | 4.661513e-49 | -8.615850e-47 |
| Msa0072720 | Msa0565170 | 0.800928 | 1.145114e-48 | -8.615850e-47 |
| Msa0097770 | Msa0565170 | 0.806026 | 1.004577e-49 | -8.615850e-47 |
| Msa0104670 | Msa0565170 | 0.810641 | 1.042042e-50 | -8.615850e-47 |
| Msa0106620 | Msa0565170 | 0.828996 | 6.588037e-55 | -8.615850e-47 |
| Msa0106710 | Msa0565170 | 0.826137 | 3.199266e-54 | -8.615850e-47 |
| Msa0108410 | Msa0565170 | 0.830292 | 3.187449e-55 | -8.615850e-47 |
| Msa0125650 | Msa0565170 | 0.812476 | 4.159617e-51 | -8.615850e-47 |
| Msa0138360 | Msa0565170 | 0.801655 | 8.128371e-49 | -8.615850e-47 |
| Msa0150860 | Msa0565170 | 0.803453 | 3.460979e-49 | -8.615850e-47 |
| Msa0183760 | Msa0565170 | 0.808567 | 2.906931e-50 | -8.615850e-47 |
| Msa0204960 | Msa0565170 | 0.810514 | 1.110080e-50 | -8.615850e-47 |
| Msa0207350 | Msa0565170 | 0.826303 | 2.920527e-54 | -8.615850e-47 |
| Msa0214550 | Msa0565170 | 0.806200 | 9.230731e-50 | -8.615850e-47 |
| Msa0231190 | Msa0565170 | 0.812099 | 5.027421e-51 | -8.615850e-47 |
| Msa0234090 | Msa0565170 | 0.809454 | 1.877140e-50 | -8.615850e-47 |
| Msa0237210 | Msa0565170 | 0.828606 | 8.187403e-55 | -8.615850e-47 |
| Msa0367870 | Msa0565170 | 0.800525 | 1.383492e-48 | -8.615850e-47 |
| Msa0378720 | Msa0565170 | 0.833947 | 3.980275e-56 | -8.615850e-47 |
| Msa0382370 | Msa0565170 | 0.800219 | 1.597302e-48 | -8.615850e-47 |
| Msa0403640 | Msa0565170 | 0.813592 | 2.367299e-51 | -8.615850e-47 |
| Msa0430750 | Msa0565170 | 0.801657 | 8.118425e-49 | -8.615850e-47 |
| Msa0432160 | Msa0565170 | 0.812969 | 3.245002e-51 | -8.615850e-47 |
| Msa0450400 | Msa0565170 | -0.828846 | 7.164083e-55 | -8.615850e-47 |
| Msa0467100 | Msa0565170 | 0.829924 | 3.919964e-55 | -8.615850e-47 |
| Msa0468410 | Msa0565170 | 0.817437 | 3.298662e-52 | -8.615850e-47 |
| Msa0474730 | Msa0565170 | 0.837088 | 6.389078e-57 | -8.615850e-47 |
| Msa0476680 | Msa0565170 | 0.818263 | 2.147254e-52 | -8.615850e-47 |
| Msa0481970 | Msa0565170 | 0.809989 | 1.440363e-50 | -8.615850e-47 |
| Msa0240900 | Msa0565170 | 0.844879 | 5.751250e-59 | -8.615850e-47 |
| Msa0241830 | Msa0565170 | 0.810707 | 1.008363e-50 | -8.615850e-47 |
| Msa0274980 | Msa0565170 | 0.804410 | 2.189101e-49 | -8.615850e-47 |
| Msa0279850 | Msa0565170 | 0.826885 | 2.122080e-54 | -8.615850e-47 |
| Msa0288080 | Msa0565170 | 0.806868 | 6.674998e-50 | -8.615850e-47 |
| Msa0300270 | Msa0565170 | 0.821907 | 3.145057e-53 | -8.615850e-47 |
| Msa0300320 | Msa0565170 | 0.808319 | 3.283446e-50 | -8.615850e-47 |
| Msa0315690 | Msa0565170 | 0.835984 | 1.220691e-56 | -8.615850e-47 |
| Msa0317970 | Msa0565170 | 0.851945 | 6.373866e-61 | -8.615850e-47 |
| Msa0322900 | Msa0565170 | 0.827612 | 1.421000e-54 | -8.615850e-47 |
| Msa0335800 | Msa0565170 | 0.803035 | 4.224381e-49 | -8.615850e-47 |
| Msa0337700 | Msa0565170 | 0.811357 | 7.290130e-51 | -8.615850e-47 |
| Msa0341690 | Msa0565170 | 0.805893 | 1.071318e-49 | -8.615850e-47 |
| Msa0485240 | Msa0565170 | 0.836900 | 7.135181e-57 | -8.615850e-47 |
| Msa0518030 | Msa0565170 | 0.821965 | 3.049858e-53 | -8.615850e-47 |
| Msa0521010 | Msa0565170 | 0.818548 | 1.850464e-52 | -8.615850e-47 |
| Msa0532960 | Msa0565170 | 0.806056 | 9.898760e-50 | -8.615850e-47 |
| Msa0556010 | Msa0565170 | 0.802467 | 5.532396e-49 | -8.615850e-47 |
| Msa0565170 | Msa0573180 | 0.800088 | 1.698288e-48 | -8.615850e-47 |
| Msa0565170 | Msa0574390 | 0.802681 | 4.998188e-49 | -8.615850e-47 |
| Msa0565170 | Msa0583360 | -0.809000 | 2.348992e-50 | -8.615850e-47 |
| Msa0565170 | Msa0601950 | 0.800305 | 1.534175e-48 | -8.615850e-47 |
| Msa0565170 | Msa0659120 | 0.801803 | 7.578022e-49 | -8.615850e-47 |
| Msa0565170 | Msa0663270 | 0.806811 | 6.860207e-50 | -8.615850e-47 |
| Msa0565170 | Msa0664540 | 0.806716 | 7.186140e-50 | -8.615850e-47 |
| Msa0565170 | Msa0693330 | 0.800518 | 1.388169e-48 | -8.615850e-47 |
| Msa0565170 | Msa0701360 | 0.883765 | 3.401779e-71 | -8.615850e-47 |
| Msa0565170 | Msa0701440 | 0.813912 | 2.012945e-51 | -8.615850e-47 |
| Msa0565170 | Msa0702720 | 0.817425 | 3.320615e-52 | -8.615850e-47 |
| Msa0565170 | Msa0709270 | 0.800258 | 1.568014e-48 | -8.615850e-47 |
| Msa0565170 | Msa0711430 | 0.822607 | 2.164086e-53 | -8.615850e-47 |
| Msa0565170 | Msa0832120 | 0.823108 | 1.653911e-53 | -8.615850e-47 |
| Msa0565170 | Msa0833530 | 0.822719 | 2.037698e-53 | -8.615850e-47 |
| Msa0565170 | Msa0871720 | 0.845737 | 3.369241e-59 | -8.615850e-47 |
| Msa0565170 | Msa0903710 | 0.803816 | 2.910316e-49 | -8.615850e-47 |
| Msa0565170 | Msa0933760 | 0.831989 | 1.220585e-55 | -8.615850e-47 |
| Msa0565170 | Msa0934140 | 0.844478 | 7.372763e-59 | -8.615850e-47 |
| Msa0565170 | Msa0936380 | 0.808454 | 3.072904e-50 | -8.615850e-47 |
| Msa0565170 | Msa0937020 | 0.826083 | 3.295152e-54 | -8.615850e-47 |
| Msa0565170 | Msa0971650 | 0.806246 | 9.029186e-50 | -8.615850e-47 |
| Msa0565170 | Msa0979180 | 0.807258 | 5.519026e-50 | -8.615850e-47 |
| Msa0565170 | Msa1012800 | 0.800913 | 1.152830e-48 | -8.615850e-47 |
| Msa0565170 | Msa1034500 | 0.857344 | 1.740477e-62 | -8.615850e-47 |
| Msa0565170 | Msa1061980 | 0.806178 | 9.332293e-50 | -8.615850e-47 |
| Msa0565170 | Msa1079230 | 0.804696 | 1.908281e-49 | -8.615850e-47 |
| Msa0565170 | Msa1089450 | 0.847907 | 8.592977e-60 | -8.615850e-47 |
| Msa0565170 | Msa1111380 | 0.823479 | 1.355048e-53 | -8.615850e-47 |
| Msa0565170 | Msa1145810 | 0.822692 | 2.067634e-53 | -8.615850e-47 |
| Msa0565170 | Msa1208430 | 0.812268 | 4.617344e-51 | -8.615850e-47 |
| Msa0565170 | Msa1225860 | -0.812796 | 3.539809e-51 | -8.615850e-47 |
| Msa0565170 | Msa1226270 | 0.834941 | 2.239345e-56 | -8.615850e-47 |
| Msa0565170 | Msa1265590 | 0.801437 | 9.008290e-49 | -8.615850e-47 |
| Msa0565170 | Msa1271130 | 0.805381 | 1.372073e-49 | -8.615850e-47 |
| Msa0565170 | Msa1277080 | 0.802484 | 5.488335e-49 | -8.615850e-47 |
| Msa0565170 | Msa1285550 | 0.833233 | 6.000521e-56 | -8.615850e-47 |
| Msa0565170 | Msa1341840 | 0.821431 | 4.052863e-53 | -8.615850e-47 |
| Msa0565170 | Msa1345810 | 0.804170 | 2.457005e-49 | -8.615850e-47 |
| Msa0565170 | Msa1348090 | 0.815668 | 8.214910e-52 | -8.615850e-47 |
| Msa0565170 | Msa1355450 | 0.803722 | 3.043134e-49 | -8.615850e-47 |
| Msa0565170 | Msa1360980 | 0.803092 | 4.110871e-49 | -8.615850e-47 |
| Msa0565170 | Msa1369690 | 0.800200 | 1.610937e-48 | -8.615850e-47 |
| Msa0565170 | Msa1410420 | 0.809167 | 2.163065e-50 | -8.615850e-47 |
| Msa0565170 | Msa1418240 | 0.840262 | 9.665812e-58 | -8.615850e-47 |
| Msa0565170 | Msa1425880 | 0.800891 | 1.164828e-48 | -8.615850e-47 |
| Msa0565170 | Msa1440310 | 0.817128 | 3.872566e-52 | -8.615850e-47 |
| Msa0565170 | Msa1454530 | 0.813155 | 2.953010e-51 | -8.615850e-47 |
| Msa0565170 | Msa1461370 | 0.800890 | 1.165538e-48 | -8.615850e-47 |
| Msa0565170 | Msa1461540 | 0.812235 | 4.694644e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0565170 | MtrunA17_Chr8g0379551 | 97.425 | 466 | 12 | 0 | 1 | 466 | 14 | 479 | 0.0 | 939 |
| Msa0565170 | MtrunA17_Chr2g0315531 | 44.766 | 449 | 237 | 5 | 14 | 458 | 9 | 450 | 1.85e-122 | 365 |
| Msa0565170 | MtrunA17_Chr8g0359791 | 48.320 | 387 | 185 | 6 | 78 | 459 | 33 | 409 | 1.49e-116 | 348 |
| Msa0565170 | MtrunA17_Chr4g0011611 | 39.447 | 398 | 215 | 6 | 74 | 459 | 30 | 413 | 5.34e-94 | 290 |
| Msa0565170 | MtrunA17_Chr7g0230481 | 33.414 | 413 | 246 | 6 | 62 | 459 | 125 | 523 | 1.01e-63 | 215 |
| Msa0565170 | MtrunA17_Chr3g0103691 | 28.715 | 397 | 256 | 5 | 79 | 458 | 91 | 477 | 5.79e-50 | 177 |
| Msa0565170 | MtrunA17_Chr5g0432511 | 31.099 | 373 | 231 | 5 | 93 | 465 | 33 | 379 | 5.17e-48 | 169 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0565170 | AT1G29300.1 | 54.054 | 481 | 184 | 9 | 1 | 466 | 1 | 459 | 7.93e-169 | 483 |
| Msa0565170 | AT3G14870.3 | 44.850 | 466 | 246 | 7 | 1 | 459 | 2 | 463 | 4.46e-131 | 387 |
| Msa0565170 | AT3G14870.4 | 44.850 | 466 | 246 | 7 | 1 | 459 | 1 | 462 | 4.61e-131 | 387 |
| Msa0565170 | AT3G14870.5 | 44.850 | 466 | 246 | 7 | 1 | 459 | 1 | 462 | 4.61e-131 | 387 |
| Msa0565170 | AT3G14870.2 | 44.850 | 466 | 246 | 7 | 1 | 459 | 1 | 462 | 4.61e-131 | 387 |
| Msa0565170 | AT3G14870.1 | 44.850 | 466 | 246 | 7 | 1 | 459 | 5 | 466 | 6.58e-131 | 387 |
| Msa0565170 | AT1G53380.2 | 43.803 | 468 | 239 | 5 | 1 | 460 | 1 | 452 | 3.67e-129 | 382 |
| Msa0565170 | AT1G53380.4 | 43.803 | 468 | 239 | 5 | 1 | 460 | 1 | 452 | 3.67e-129 | 382 |
| Msa0565170 | AT1G53380.1 | 43.803 | 468 | 239 | 5 | 1 | 460 | 1 | 452 | 3.67e-129 | 382 |
| Msa0565170 | AT1G53380.3 | 43.803 | 468 | 239 | 5 | 1 | 460 | 1 | 452 | 3.67e-129 | 382 |
| Msa0565170 | AT1G53380.5 | 43.803 | 468 | 239 | 5 | 1 | 460 | 1 | 452 | 3.67e-129 | 382 |
| Msa0565170 | AT2G45260.1 | 40.100 | 399 | 220 | 4 | 74 | 461 | 31 | 421 | 1.97e-96 | 297 |
| Msa0565170 | AT3G60680.1 | 34.625 | 387 | 233 | 5 | 81 | 459 | 120 | 494 | 1.04e-71 | 235 |
| Msa0565170 | AT2G30380.1 | 32.663 | 398 | 217 | 8 | 67 | 459 | 60 | 411 | 1.09e-55 | 191 |
| Msa0565170 | AT4G34080.1 | 36.364 | 275 | 164 | 3 | 74 | 337 | 7 | 281 | 3.16e-53 | 180 |
| Msa0565170 | AT4G33320.1 | 35.448 | 268 | 161 | 4 | 74 | 329 | 7 | 274 | 5.14e-48 | 167 |
| Msa0565170 | AT5G58960.2 | 29.557 | 406 | 250 | 7 | 79 | 458 | 85 | 480 | 3.10e-43 | 159 |
| Msa0565170 | AT5G58960.3 | 29.557 | 406 | 250 | 7 | 79 | 458 | 85 | 480 | 3.10e-43 | 159 |
| Msa0565170 | AT2G32130.1 | 57.724 | 123 | 52 | 0 | 80 | 202 | 34 | 156 | 6.34e-43 | 149 |
| Msa0565170 | AT5G58960.1 | 29.557 | 406 | 250 | 7 | 79 | 458 | 160 | 555 | 9.32e-43 | 159 |
| Msa0565170 | AT4G36100.1 | 43.636 | 110 | 57 | 2 | 74 | 178 | 7 | 116 | 7.78e-21 | 91.7 |
Find 59 sgRNAs with CRISPR-Local
Find 156 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCTTGTTCATTGCTGATTT+TGG | 0.079747 | 4_1:+66096003 | None:intergenic |
| TTCTAATTCCGGCGAGTTTC+GGG | 0.120775 | 4_1:-66094732 | Msa0565170:CDS |
| ATGGACAACTTGATGATATT+TGG | 0.163563 | 4_1:+66095012 | None:intergenic |
| ATTCTAATTCCGGCGAGTTT+CGG | 0.208130 | 4_1:-66094733 | Msa0565170:CDS |
| TCATTTGAAACAGTGAAATT+TGG | 0.218352 | 4_1:+66095154 | None:intergenic |
| ATTTGTCAAAATAGTGATTT+TGG | 0.234573 | 4_1:+66095113 | None:intergenic |
| CGGAGATGGCAAAGCGTGTT+TGG | 0.284189 | 4_1:-66094895 | Msa0565170:CDS |
| GATTCTATTGATTCTAATTC+CGG | 0.300156 | 4_1:-66094743 | Msa0565170:CDS |
| TCAGTTTCAACTTCATGTTC+AGG | 0.306259 | 4_1:-66094767 | Msa0565170:CDS |
| TTCTCCTTGAAGCTTCTTGA+TGG | 0.321429 | 4_1:+66095528 | None:intergenic |
| CTTCTTGATGGTGATTTCAT+AGG | 0.327494 | 4_1:+66095540 | None:intergenic |
| TTCTTATTGATCTCAAAGTA+TGG | 0.329293 | 4_1:+66095338 | None:intergenic |
| GAGTCAGGTTTATCTGTCTT+TGG | 0.330222 | 4_1:-66094660 | Msa0565170:CDS |
| CAGAGGAAGCTAGTGAATTC+TGG | 0.337395 | 4_1:-66094953 | Msa0565170:CDS |
| TCAAGTTGTCCATGCTAAAA+TGG | 0.340049 | 4_1:-66095002 | Msa0565170:CDS |
| GAAACTTATGATTGAAGAAA+TGG | 0.349019 | 4_1:-66095308 | Msa0565170:CDS |
| AAAATGGAGTGTTCTTTGTT+TGG | 0.368125 | 4_1:-66094986 | Msa0565170:CDS |
| AAGAAATGGAATCAGCAAAC+TGG | 0.369176 | 4_1:-66095294 | Msa0565170:CDS |
| GATTCAACATTGAATGTTCA+AGG | 0.385942 | 4_1:+66095386 | None:intergenic |
| AACTTGGCAAAAGATGAGTT+TGG | 0.414833 | 4_1:+66095043 | None:intergenic |
| TTCTAACCTTGTTCTTCTGT+TGG | 0.416097 | 4_1:+66095818 | None:intergenic |
| GGTTAGAAACAAAGCAGTTA+TGG | 0.418035 | 4_1:-66095803 | Msa0565170:CDS |
| TGTTTGGACACTGCATTACT+TGG | 0.442751 | 4_1:-66094879 | Msa0565170:CDS |
| AAAACAACGATGACTTGGTT+TGG | 0.448235 | 4_1:+66095225 | None:intergenic |
| CAGTTTCAACTTCATGTTCA+GGG | 0.450542 | 4_1:-66094766 | Msa0565170:CDS |
| ATCATATGCAGAACTTCAAA+TGG | 0.451722 | 4_1:-66095734 | Msa0565170:CDS |
| TAGGTTCTCTGAGGTTTACA+TGG | 0.451972 | 4_1:-66094807 | Msa0565170:CDS |
| ATCACCATCAAGAAGCTTCA+AGG | 0.452355 | 4_1:-66095532 | Msa0565170:CDS |
| CAGAATTCACTAGCTTCCTC+TGG | 0.453524 | 4_1:+66094954 | None:intergenic |
| TCCAGGTCAAGAAGAATACT+AGG | 0.462182 | 4_1:-66094826 | Msa0565170:CDS |
| TGTATTGAATCATTGTTGTA+AGG | 0.470099 | 4_1:+66095703 | None:intergenic |
| TAAGACAGTGATACAGAGTC+AGG | 0.472174 | 4_1:-66094675 | Msa0565170:CDS |
| TCAGGTTTATCTGTCTTTGG+TGG | 0.472612 | 4_1:-66094657 | Msa0565170:CDS |
| GGTTTGGTGAAAACTGCGTT+TGG | 0.476936 | 4_1:+66095241 | None:intergenic |
| CCATCTCAGAATTGAAAAGA+AGG | 0.510329 | 4_1:-66095645 | Msa0565170:CDS |
| AAAGCAAAACAACGATGACT+TGG | 0.515541 | 4_1:+66095220 | None:intergenic |
| AGAAATGGAATCAGCAAACT+GGG | 0.539408 | 4_1:-66095293 | Msa0565170:CDS |
| TTTGGTGAATATTGTGAAGA+GGG | 0.541008 | 4_1:+66095131 | None:intergenic |
| ACCTAGTATTCTTCTTGACC+TGG | 0.541417 | 4_1:+66094825 | None:intergenic |
| CAAAATCAGCAATGAACAAG+AGG | 0.553083 | 4_1:-66096002 | Msa0565170:CDS |
| TGGTGAAAACTGCGTTTGGA+TGG | 0.556904 | 4_1:+66095245 | None:intergenic |
| GTGGTGCCTGGTTTCAACAT+TGG | 0.564074 | 4_1:-66094698 | Msa0565170:CDS |
| TAATTCCGGCGAGTTTCGGG+TGG | 0.572386 | 4_1:-66094729 | Msa0565170:CDS |
| ATTTGATGATGATCTGTGAA+AGG | 0.573153 | 4_1:+66095859 | None:intergenic |
| AGCAGCTGATCAAGCAGTGG+TGG | 0.584305 | 4_1:-66095680 | Msa0565170:CDS |
| GAAAACCACCCGAAACTCGC+CGG | 0.587240 | 4_1:+66094724 | None:intergenic |
| AGTTTCAACTTCATGTTCAG+GGG | 0.594405 | 4_1:-66094765 | Msa0565170:CDS |
| CAACTCACATCTCAAAGTGA+AGG | 0.600233 | 4_1:-66095893 | Msa0565170:CDS |
| TGTTTGGAAATTTGAACCAG+AGG | 0.621071 | 4_1:-66094970 | Msa0565170:CDS |
| ATTTGCAAAGACATTCCAGA+AGG | 0.624146 | 4_1:-66095971 | Msa0565170:CDS |
| TTATGGAGGCTCTAATAGCA+AGG | 0.629498 | 4_1:-66095786 | Msa0565170:CDS |
| AGGAAGCTAGTGAATTCTGG+AGG | 0.632294 | 4_1:-66094950 | Msa0565170:CDS |
| GCTAGCATTATAGTAACTTG+AGG | 0.633614 | 4_1:+66095595 | None:intergenic |
| GTCTTACCAATGTTGAAACC+AGG | 0.633731 | 4_1:+66094692 | None:intergenic |
| TAGAAACAAAGCAGTTATGG+AGG | 0.646650 | 4_1:-66095800 | Msa0565170:CDS |
| GAAGAATACTAGGTTCTCTG+AGG | 0.672182 | 4_1:-66094816 | Msa0565170:CDS |
| GGAAGCTAGTGAATTCTGGA+GGG | 0.675270 | 4_1:-66094949 | Msa0565170:CDS |
| ACAAGCAGCTGATCAAGCAG+TGG | 0.679340 | 4_1:-66095683 | Msa0565170:CDS |
| TAAGCACCAACAGAAGAACA+AGG | 0.710444 | 4_1:-66095824 | Msa0565170:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAAAAATTGCTTCAAATAA+TGG | - | chr4_1:66094727-66094746 | Msa0565170:CDS | 20.0% |
| !!! | ATTTGTCAAAATAGTGATTT+TGG | + | chr4_1:66095545-66095564 | None:intergenic | 20.0% |
| !!! | ATATTTGGATTTTAGAAACT+TGG | + | chr4_1:66095631-66095650 | None:intergenic | 20.0% |
| !! | ACAAAAATTGCTTCAAATAA+TGG | - | chr4_1:66094727-66094746 | Msa0565170:CDS | 20.0% |
| !!! | ATTTGTCAAAATAGTGATTT+TGG | + | chr4_1:66095545-66095564 | None:intergenic | 20.0% |
| !!! | ATATTTGGATTTTAGAAACT+TGG | + | chr4_1:66095631-66095650 | None:intergenic | 20.0% |
| ! | TGTATTGAATCATTGTTGTA+AGG | + | chr4_1:66094955-66094974 | None:intergenic | 25.0% |
| ! | TTCTTATTGATCTCAAAGTA+TGG | + | chr4_1:66095320-66095339 | None:intergenic | 25.0% |
| ! | GAAACTTATGATTGAAGAAA+TGG | - | chr4_1:66095347-66095366 | Msa0565170:CDS | 25.0% |
| !! | TCATTTGAAACAGTGAAATT+TGG | + | chr4_1:66095504-66095523 | None:intergenic | 25.0% |
| !!! | ACTATTTTGACAAATTCAAG+AGG | - | chr4_1:66095550-66095569 | Msa0565170:CDS | 25.0% |
| !!! | TTGTTTTCTAAGTACTGTTT+TGG | + | chr4_1:66095591-66095610 | None:intergenic | 25.0% |
| !!! | GATTCTATTGATTCTAATTC+CGG | - | chr4_1:66095912-66095931 | Msa0565170:CDS | 25.0% |
| ! | TGTATTGAATCATTGTTGTA+AGG | + | chr4_1:66094955-66094974 | None:intergenic | 25.0% |
| ! | TTCTTATTGATCTCAAAGTA+TGG | + | chr4_1:66095320-66095339 | None:intergenic | 25.0% |
| ! | GAAACTTATGATTGAAGAAA+TGG | - | chr4_1:66095347-66095366 | Msa0565170:CDS | 25.0% |
| !! | TCATTTGAAACAGTGAAATT+TGG | + | chr4_1:66095504-66095523 | None:intergenic | 25.0% |
| !!! | ACTATTTTGACAAATTCAAG+AGG | - | chr4_1:66095550-66095569 | Msa0565170:CDS | 25.0% |
| !!! | TTGTTTTCTAAGTACTGTTT+TGG | + | chr4_1:66095591-66095610 | None:intergenic | 25.0% |
| !!! | GATTCTATTGATTCTAATTC+CGG | - | chr4_1:66095912-66095931 | Msa0565170:CDS | 25.0% |
| !! | ATTTGATGATGATCTGTGAA+AGG | + | chr4_1:66094799-66094818 | None:intergenic | 30.0% |
| ! | TTCTGCATATGATGCTTTTA+TGG | + | chr4_1:66094914-66094933 | None:intergenic | 30.0% |
| ATCATATGCAGAACTTCAAA+TGG | - | chr4_1:66094921-66094940 | Msa0565170:CDS | 30.0% | |
| !!! | CTCATCAAGTTTTTTTCTGA+GGG | + | chr4_1:66095181-66095200 | None:intergenic | 30.0% |
| !!! | ACTCATCAAGTTTTTTTCTG+AGG | + | chr4_1:66095182-66095201 | None:intergenic | 30.0% |
| GATTCAACATTGAATGTTCA+AGG | + | chr4_1:66095272-66095291 | None:intergenic | 30.0% | |
| TTTGGTGAATATTGTGAAGA+GGG | + | chr4_1:66095527-66095546 | None:intergenic | 30.0% | |
| ! | TTTTGGTGAATATTGTGAAG+AGG | + | chr4_1:66095528-66095547 | None:intergenic | 30.0% |
| ! | ATGGACAACTTGATGATATT+TGG | + | chr4_1:66095646-66095665 | None:intergenic | 30.0% |
| ! | AAAATGGAGTGTTCTTTGTT+TGG | - | chr4_1:66095669-66095688 | Msa0565170:CDS | 30.0% |
| !!! | TGCTTTTTTCTTAGCTTTTG+CGG | - | chr4_1:66095740-66095759 | Msa0565170:CDS | 30.0% |
| !! | ATTTGATGATGATCTGTGAA+AGG | + | chr4_1:66094799-66094818 | None:intergenic | 30.0% |
| ! | TTCTGCATATGATGCTTTTA+TGG | + | chr4_1:66094914-66094933 | None:intergenic | 30.0% |
| ATCATATGCAGAACTTCAAA+TGG | - | chr4_1:66094921-66094940 | Msa0565170:CDS | 30.0% | |
| !!! | CTCATCAAGTTTTTTTCTGA+GGG | + | chr4_1:66095181-66095200 | None:intergenic | 30.0% |
| !!! | ACTCATCAAGTTTTTTTCTG+AGG | + | chr4_1:66095182-66095201 | None:intergenic | 30.0% |
| GATTCAACATTGAATGTTCA+AGG | + | chr4_1:66095272-66095291 | None:intergenic | 30.0% | |
| TTTGGTGAATATTGTGAAGA+GGG | + | chr4_1:66095527-66095546 | None:intergenic | 30.0% | |
| ! | TTTTGGTGAATATTGTGAAG+AGG | + | chr4_1:66095528-66095547 | None:intergenic | 30.0% |
| ! | ATGGACAACTTGATGATATT+TGG | + | chr4_1:66095646-66095665 | None:intergenic | 30.0% |
| ! | AAAATGGAGTGTTCTTTGTT+TGG | - | chr4_1:66095669-66095688 | Msa0565170:CDS | 30.0% |
| !!! | TGCTTTTTTCTTAGCTTTTG+CGG | - | chr4_1:66095740-66095759 | Msa0565170:CDS | 30.0% |
| !!! | CTCTTGTTCATTGCTGATTT+TGG | + | chr4_1:66094655-66094674 | None:intergenic | 35.0% |
| CAAAATCAGCAATGAACAAG+AGG | - | chr4_1:66094653-66094672 | Msa0565170:CDS | 35.0% | |
| ATTTGCAAAGACATTCCAGA+AGG | - | chr4_1:66094684-66094703 | Msa0565170:CDS | 35.0% | |
| !!! | TTTTGAGACTAATCACCTTC+TGG | + | chr4_1:66094702-66094721 | None:intergenic | 35.0% |
| TTCTAACCTTGTTCTTCTGT+TGG | + | chr4_1:66094840-66094859 | None:intergenic | 35.0% | |
| GGTTAGAAACAAAGCAGTTA+TGG | - | chr4_1:66094852-66094871 | Msa0565170:CDS | 35.0% | |
| TAGAAACAAAGCAGTTATGG+AGG | - | chr4_1:66094855-66094874 | Msa0565170:CDS | 35.0% | |
| !! | CCTTCTTTTCAATTCTGAGA+TGG | + | chr4_1:66095013-66095032 | None:intergenic | 35.0% |
| CCATCTCAGAATTGAAAAGA+AGG | - | chr4_1:66095010-66095029 | Msa0565170:CDS | 35.0% | |
| GCTAGCATTATAGTAACTTG+AGG | + | chr4_1:66095063-66095082 | None:intergenic | 35.0% | |
| !! | CTTCTTGATGGTGATTTCAT+AGG | + | chr4_1:66095118-66095137 | None:intergenic | 35.0% |
| AAGAAATGGAATCAGCAAAC+TGG | - | chr4_1:66095361-66095380 | Msa0565170:CDS | 35.0% | |
| AGAAATGGAATCAGCAAACT+GGG | - | chr4_1:66095362-66095381 | Msa0565170:CDS | 35.0% | |
| !! | AAAACAACGATGACTTGGTT+TGG | + | chr4_1:66095433-66095452 | None:intergenic | 35.0% |
| AAAGCAAAACAACGATGACT+TGG | + | chr4_1:66095438-66095457 | None:intergenic | 35.0% | |
| AACTTGGCAAAAGATGAGTT+TGG | + | chr4_1:66095615-66095634 | None:intergenic | 35.0% | |
| ! | TCAAGTTGTCCATGCTAAAA+TGG | - | chr4_1:66095653-66095672 | Msa0565170:CDS | 35.0% |
| !! | AAGAACACTCCATTTTAGCA+TGG | + | chr4_1:66095665-66095684 | None:intergenic | 35.0% |
| TGTTTGGAAATTTGAACCAG+AGG | - | chr4_1:66095685-66095704 | Msa0565170:CDS | 35.0% | |
| GCTAAGAAAAAAGCAGAATC+TGG | + | chr4_1:66095735-66095754 | None:intergenic | 35.0% | |
| ! | AGAAGATGTTAGCATTTTCC+AGG | - | chr4_1:66095812-66095831 | Msa0565170:CDS | 35.0% |
| TCAGTTTCAACTTCATGTTC+AGG | - | chr4_1:66095888-66095907 | Msa0565170:CDS | 35.0% | |
| CAGTTTCAACTTCATGTTCA+GGG | - | chr4_1:66095889-66095908 | Msa0565170:CDS | 35.0% | |
| AGTTTCAACTTCATGTTCAG+GGG | - | chr4_1:66095890-66095909 | Msa0565170:CDS | 35.0% | |
| !!! | CTCTTGTTCATTGCTGATTT+TGG | + | chr4_1:66094655-66094674 | None:intergenic | 35.0% |
| CAAAATCAGCAATGAACAAG+AGG | - | chr4_1:66094653-66094672 | Msa0565170:CDS | 35.0% | |
| ATTTGCAAAGACATTCCAGA+AGG | - | chr4_1:66094684-66094703 | Msa0565170:CDS | 35.0% | |
| !!! | TTTTGAGACTAATCACCTTC+TGG | + | chr4_1:66094702-66094721 | None:intergenic | 35.0% |
| TTCTAACCTTGTTCTTCTGT+TGG | + | chr4_1:66094840-66094859 | None:intergenic | 35.0% | |
| GGTTAGAAACAAAGCAGTTA+TGG | - | chr4_1:66094852-66094871 | Msa0565170:CDS | 35.0% | |
| TAGAAACAAAGCAGTTATGG+AGG | - | chr4_1:66094855-66094874 | Msa0565170:CDS | 35.0% | |
| !! | CCTTCTTTTCAATTCTGAGA+TGG | + | chr4_1:66095013-66095032 | None:intergenic | 35.0% |
| CCATCTCAGAATTGAAAAGA+AGG | - | chr4_1:66095010-66095029 | Msa0565170:CDS | 35.0% | |
| GCTAGCATTATAGTAACTTG+AGG | + | chr4_1:66095063-66095082 | None:intergenic | 35.0% | |
| !! | CTTCTTGATGGTGATTTCAT+AGG | + | chr4_1:66095118-66095137 | None:intergenic | 35.0% |
| AAGAAATGGAATCAGCAAAC+TGG | - | chr4_1:66095361-66095380 | Msa0565170:CDS | 35.0% | |
| AGAAATGGAATCAGCAAACT+GGG | - | chr4_1:66095362-66095381 | Msa0565170:CDS | 35.0% | |
| !! | AAAACAACGATGACTTGGTT+TGG | + | chr4_1:66095433-66095452 | None:intergenic | 35.0% |
| AAAGCAAAACAACGATGACT+TGG | + | chr4_1:66095438-66095457 | None:intergenic | 35.0% | |
| AACTTGGCAAAAGATGAGTT+TGG | + | chr4_1:66095615-66095634 | None:intergenic | 35.0% | |
| ! | TCAAGTTGTCCATGCTAAAA+TGG | - | chr4_1:66095653-66095672 | Msa0565170:CDS | 35.0% |
| !! | AAGAACACTCCATTTTAGCA+TGG | + | chr4_1:66095665-66095684 | None:intergenic | 35.0% |
| TGTTTGGAAATTTGAACCAG+AGG | - | chr4_1:66095685-66095704 | Msa0565170:CDS | 35.0% | |
| GCTAAGAAAAAAGCAGAATC+TGG | + | chr4_1:66095735-66095754 | None:intergenic | 35.0% | |
| ! | AGAAGATGTTAGCATTTTCC+AGG | - | chr4_1:66095812-66095831 | Msa0565170:CDS | 35.0% |
| TCAGTTTCAACTTCATGTTC+AGG | - | chr4_1:66095888-66095907 | Msa0565170:CDS | 35.0% | |
| CAGTTTCAACTTCATGTTCA+GGG | - | chr4_1:66095889-66095908 | Msa0565170:CDS | 35.0% | |
| AGTTTCAACTTCATGTTCAG+GGG | - | chr4_1:66095890-66095909 | Msa0565170:CDS | 35.0% | |
| CAACTCACATCTCAAAGTGA+AGG | - | chr4_1:66094762-66094781 | Msa0565170:CDS | 40.0% | |
| TAAGCACCAACAGAAGAACA+AGG | - | chr4_1:66094831-66094850 | Msa0565170:CDS | 40.0% | |
| ! | TTATGGAGGCTCTAATAGCA+AGG | - | chr4_1:66094869-66094888 | Msa0565170:CDS | 40.0% |
| ! | CTAATAGCAAGGCTTTTTGC+TGG | - | chr4_1:66094880-66094899 | Msa0565170:CDS | 40.0% |
| ATCACCATCAAGAAGCTTCA+AGG | - | chr4_1:66095123-66095142 | Msa0565170:CDS | 40.0% | |
| TTCTCCTTGAAGCTTCTTGA+TGG | + | chr4_1:66095130-66095149 | None:intergenic | 40.0% | |
| !! | TTTCTTAGCTTTTGCGGAGA+TGG | - | chr4_1:66095746-66095765 | Msa0565170:CDS | 40.0% |
| TGTTTGGACACTGCATTACT+TGG | - | chr4_1:66095776-66095795 | Msa0565170:CDS | 40.0% | |
| TCCAGGTCAAGAAGAATACT+AGG | - | chr4_1:66095829-66095848 | Msa0565170:CDS | 40.0% | |
| ACCTAGTATTCTTCTTGACC+TGG | + | chr4_1:66095833-66095852 | None:intergenic | 40.0% | |
| GAAGAATACTAGGTTCTCTG+AGG | - | chr4_1:66095839-66095858 | Msa0565170:CDS | 40.0% | |
| !! | TAGGTTCTCTGAGGTTTACA+TGG | - | chr4_1:66095848-66095867 | Msa0565170:CDS | 40.0% |
| ATTCTAATTCCGGCGAGTTT+CGG | - | chr4_1:66095922-66095941 | Msa0565170:CDS | 40.0% | |
| GTCTTACCAATGTTGAAACC+AGG | + | chr4_1:66095966-66095985 | None:intergenic | 40.0% | |
| TAAGACAGTGATACAGAGTC+AGG | - | chr4_1:66095980-66095999 | Msa0565170:CDS | 40.0% | |
| GAGTCAGGTTTATCTGTCTT+TGG | - | chr4_1:66095995-66096014 | Msa0565170:CDS | 40.0% | |
| TCAGGTTTATCTGTCTTTGG+TGG | - | chr4_1:66095998-66096017 | Msa0565170:CDS | 40.0% | |
| CAACTCACATCTCAAAGTGA+AGG | - | chr4_1:66094762-66094781 | Msa0565170:CDS | 40.0% | |
| TAAGCACCAACAGAAGAACA+AGG | - | chr4_1:66094831-66094850 | Msa0565170:CDS | 40.0% | |
| ! | TTATGGAGGCTCTAATAGCA+AGG | - | chr4_1:66094869-66094888 | Msa0565170:CDS | 40.0% |
| ! | CTAATAGCAAGGCTTTTTGC+TGG | - | chr4_1:66094880-66094899 | Msa0565170:CDS | 40.0% |
| ATCACCATCAAGAAGCTTCA+AGG | - | chr4_1:66095123-66095142 | Msa0565170:CDS | 40.0% | |
| TTCTCCTTGAAGCTTCTTGA+TGG | + | chr4_1:66095130-66095149 | None:intergenic | 40.0% | |
| !! | TTTCTTAGCTTTTGCGGAGA+TGG | - | chr4_1:66095746-66095765 | Msa0565170:CDS | 40.0% |
| TGTTTGGACACTGCATTACT+TGG | - | chr4_1:66095776-66095795 | Msa0565170:CDS | 40.0% | |
| TCCAGGTCAAGAAGAATACT+AGG | - | chr4_1:66095829-66095848 | Msa0565170:CDS | 40.0% | |
| ACCTAGTATTCTTCTTGACC+TGG | + | chr4_1:66095833-66095852 | None:intergenic | 40.0% | |
| GAAGAATACTAGGTTCTCTG+AGG | - | chr4_1:66095839-66095858 | Msa0565170:CDS | 40.0% | |
| !! | TAGGTTCTCTGAGGTTTACA+TGG | - | chr4_1:66095848-66095867 | Msa0565170:CDS | 40.0% |
| ATTCTAATTCCGGCGAGTTT+CGG | - | chr4_1:66095922-66095941 | Msa0565170:CDS | 40.0% | |
| GTCTTACCAATGTTGAAACC+AGG | + | chr4_1:66095966-66095985 | None:intergenic | 40.0% | |
| TAAGACAGTGATACAGAGTC+AGG | - | chr4_1:66095980-66095999 | Msa0565170:CDS | 40.0% | |
| GAGTCAGGTTTATCTGTCTT+TGG | - | chr4_1:66095995-66096014 | Msa0565170:CDS | 40.0% | |
| TCAGGTTTATCTGTCTTTGG+TGG | - | chr4_1:66095998-66096017 | Msa0565170:CDS | 40.0% | |
| TGGTGAAAACTGCGTTTGGA+TGG | + | chr4_1:66095413-66095432 | None:intergenic | 45.0% | |
| !! | GGTTTGGTGAAAACTGCGTT+TGG | + | chr4_1:66095417-66095436 | None:intergenic | 45.0% |
| ! | CAGAATTCACTAGCTTCCTC+TGG | + | chr4_1:66095704-66095723 | None:intergenic | 45.0% |
| CAGAGGAAGCTAGTGAATTC+TGG | - | chr4_1:66095702-66095721 | Msa0565170:CDS | 45.0% | |
| AGGAAGCTAGTGAATTCTGG+AGG | - | chr4_1:66095705-66095724 | Msa0565170:CDS | 45.0% | |
| GGAAGCTAGTGAATTCTGGA+GGG | - | chr4_1:66095706-66095725 | Msa0565170:CDS | 45.0% | |
| TTCTAATTCCGGCGAGTTTC+GGG | - | chr4_1:66095923-66095942 | Msa0565170:CDS | 45.0% | |
| TGGTGAAAACTGCGTTTGGA+TGG | + | chr4_1:66095413-66095432 | None:intergenic | 45.0% | |
| !! | GGTTTGGTGAAAACTGCGTT+TGG | + | chr4_1:66095417-66095436 | None:intergenic | 45.0% |
| ! | CAGAATTCACTAGCTTCCTC+TGG | + | chr4_1:66095704-66095723 | None:intergenic | 45.0% |
| CAGAGGAAGCTAGTGAATTC+TGG | - | chr4_1:66095702-66095721 | Msa0565170:CDS | 45.0% | |
| AGGAAGCTAGTGAATTCTGG+AGG | - | chr4_1:66095705-66095724 | Msa0565170:CDS | 45.0% | |
| GGAAGCTAGTGAATTCTGGA+GGG | - | chr4_1:66095706-66095725 | Msa0565170:CDS | 45.0% | |
| TTCTAATTCCGGCGAGTTTC+GGG | - | chr4_1:66095923-66095942 | Msa0565170:CDS | 45.0% | |
| ACAAGCAGCTGATCAAGCAG+TGG | - | chr4_1:66094972-66094991 | Msa0565170:CDS | 50.0% | |
| !! | CGAGTTTCGGGTGGTTTTCA+CGG | - | chr4_1:66095935-66095954 | Msa0565170:CDS | 50.0% |
| GTGGTGCCTGGTTTCAACAT+TGG | - | chr4_1:66095957-66095976 | Msa0565170:CDS | 50.0% | |
| ACAAGCAGCTGATCAAGCAG+TGG | - | chr4_1:66094972-66094991 | Msa0565170:CDS | 50.0% | |
| !! | CGAGTTTCGGGTGGTTTTCA+CGG | - | chr4_1:66095935-66095954 | Msa0565170:CDS | 50.0% |
| GTGGTGCCTGGTTTCAACAT+TGG | - | chr4_1:66095957-66095976 | Msa0565170:CDS | 50.0% | |
| AGCAGCTGATCAAGCAGTGG+TGG | - | chr4_1:66094975-66094994 | Msa0565170:CDS | 55.0% | |
| CGGAGATGGCAAAGCGTGTT+TGG | - | chr4_1:66095760-66095779 | Msa0565170:CDS | 55.0% | |
| TAATTCCGGCGAGTTTCGGG+TGG | - | chr4_1:66095926-66095945 | Msa0565170:CDS | 55.0% | |
| GAAAACCACCCGAAACTCGC+CGG | + | chr4_1:66095934-66095953 | None:intergenic | 55.0% | |
| !! | GTTTCGGGTGGTTTTCACGG+TGG | - | chr4_1:66095938-66095957 | Msa0565170:CDS | 55.0% |
| AGCAGCTGATCAAGCAGTGG+TGG | - | chr4_1:66094975-66094994 | Msa0565170:CDS | 55.0% | |
| CGGAGATGGCAAAGCGTGTT+TGG | - | chr4_1:66095760-66095779 | Msa0565170:CDS | 55.0% | |
| TAATTCCGGCGAGTTTCGGG+TGG | - | chr4_1:66095926-66095945 | Msa0565170:CDS | 55.0% | |
| GAAAACCACCCGAAACTCGC+CGG | + | chr4_1:66095934-66095953 | None:intergenic | 55.0% | |
| !! | GTTTCGGGTGGTTTTCACGG+TGG | - | chr4_1:66095938-66095957 | Msa0565170:CDS | 55.0% |
| ! | GTGGTTTTCACGGTGGTGCC+TGG | - | chr4_1:66095945-66095964 | Msa0565170:CDS | 60.0% |
| ! | GTGGTTTTCACGGTGGTGCC+TGG | - | chr4_1:66095945-66095964 | Msa0565170:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_1 | gene | 66094637 | 66096040 | 66094637 | ID=Msa0565170;Name=Msa0565170 |
| chr4_1 | mRNA | 66094637 | 66096040 | 66094637 | ID=Msa0565170-mRNA-1;Parent=Msa0565170;Name=Msa0565170-mRNA-1;_AED=0.07;_eAED=0.07;_QI=0|-1|0|1|-1|1|1|0|467 |
| chr4_1 | exon | 66094637 | 66096040 | 66094637 | ID=Msa0565170-mRNA-1:exon:21383;Parent=Msa0565170-mRNA-1 |
| chr4_1 | CDS | 66094637 | 66096040 | 66094637 | ID=Msa0565170-mRNA-1:cds;Parent=Msa0565170-mRNA-1 |
| Gene Sequence |
| Protein sequence |
