Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0701360 | RHN42672.1 | 97.639 | 466 | 11 | 0 | 1 | 466 | 14 | 479 | 0.0 | 943 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0701360 | sp|F4KGE8|GIL1_ARATH | 29.557 | 406 | 250 | 7 | 79 | 458 | 160 | 555 | 1.76e-42 | 161 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0701360 | A0A396GNK6 | 97.639 | 466 | 11 | 0 | 1 | 466 | 14 | 479 | 0.0 | 943 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0030160 | Msa0701360 | 0.812816 | 3.503915e-51 | -8.615850e-47 |
| Msa0035120 | Msa0701360 | 0.811997 | 5.291965e-51 | -8.615850e-47 |
| Msa0093850 | Msa0701360 | 0.815067 | 1.117660e-51 | -8.615850e-47 |
| Msa0097770 | Msa0701360 | 0.808979 | 2.373301e-50 | -8.615850e-47 |
| Msa0106620 | Msa0701360 | 0.825166 | 5.436574e-54 | -8.615850e-47 |
| Msa0106710 | Msa0701360 | 0.820734 | 5.865026e-53 | -8.615850e-47 |
| Msa0108410 | Msa0701360 | 0.818254 | 2.157763e-52 | -8.615850e-47 |
| Msa0663270 | Msa0701360 | 0.840613 | 7.827180e-58 | -8.615850e-47 |
| Msa0664090 | Msa0701360 | 0.803945 | 2.735820e-49 | -8.615850e-47 |
| Msa0664540 | Msa0701360 | 0.805892 | 1.071668e-49 | -8.615850e-47 |
| Msa0693330 | Msa0701360 | 0.831674 | 1.460057e-55 | -8.615850e-47 |
| Msa0701360 | Msa0701440 | 0.848956 | 4.403653e-60 | -8.615850e-47 |
| Msa0701360 | Msa0702720 | 0.831210 | 1.899770e-55 | -8.615850e-47 |
| Msa0701360 | Msa0705120 | 0.804489 | 2.107868e-49 | -8.615850e-47 |
| Msa0701360 | Msa0705580 | 0.827950 | 1.178693e-54 | -8.615850e-47 |
| Msa0701360 | Msa0713680 | 0.806036 | 9.997267e-50 | -8.615850e-47 |
| Msa0701360 | Msa0715060 | 0.819998 | 8.652113e-53 | -8.615850e-47 |
| Msa0701360 | Msa0822500 | 0.800741 | 1.250054e-48 | -8.615850e-47 |
| Msa0701360 | Msa0833530 | 0.840002 | 1.129924e-57 | -8.615850e-47 |
| Msa0701360 | Msa0871720 | 0.815035 | 1.136060e-51 | -8.615850e-47 |
| Msa0701360 | Msa0934140 | 0.802653 | 5.065369e-49 | -8.615850e-47 |
| Msa0701360 | Msa0936380 | 0.803070 | 4.154433e-49 | -8.615850e-47 |
| Msa0701360 | Msa0987840 | 0.825684 | 4.098073e-54 | -8.615850e-47 |
| Msa0701360 | Msa1023190 | 0.802558 | 5.299874e-49 | -8.615850e-47 |
| Msa0701360 | Msa1034500 | 0.825253 | 5.184656e-54 | -8.615850e-47 |
| Msa0701360 | Msa1061980 | 0.822611 | 2.159058e-53 | -8.615850e-47 |
| Msa0701360 | Msa1079230 | 0.805765 | 1.140003e-49 | -8.615850e-47 |
| Msa0701360 | Msa1089450 | 0.853703 | 2.005075e-61 | -8.615850e-47 |
| Msa0701360 | Msa1111380 | 0.810436 | 1.153710e-50 | -8.615850e-47 |
| Msa0701360 | Msa1145810 | 0.827951 | 1.178067e-54 | -8.615850e-47 |
| Msa0701360 | Msa1155170 | -0.802470 | 5.524466e-49 | -8.615850e-47 |
| Msa0701360 | Msa1162770 | 0.808943 | 2.416007e-50 | -8.615850e-47 |
| Msa0701360 | Msa1173300 | 0.823680 | 1.215932e-53 | -8.615850e-47 |
| Msa0701360 | Msa1204100 | -0.828254 | 9.956234e-55 | -8.615850e-47 |
| Msa0701360 | Msa1209440 | 0.801095 | 1.058282e-48 | -8.615850e-47 |
| Msa0701360 | Msa1219000 | 0.815316 | 9.841831e-52 | -8.615850e-47 |
| Msa0701360 | Msa1225860 | -0.829219 | 5.816421e-55 | -8.615850e-47 |
| Msa0701360 | Msa1226270 | 0.811452 | 6.953565e-51 | -8.615850e-47 |
| Msa0701360 | Msa1265590 | 0.807797 | 4.240302e-50 | -8.615850e-47 |
| Msa0701360 | Msa1275590 | 0.818804 | 1.619361e-52 | -8.615850e-47 |
| Msa0701360 | Msa1277080 | 0.802846 | 4.621931e-49 | -8.615850e-47 |
| Msa0701360 | Msa1329050 | -0.825684 | 4.099821e-54 | -8.615850e-47 |
| Msa0701360 | Msa1332760 | 0.801275 | 9.722187e-49 | -8.615850e-47 |
| Msa0701360 | Msa1335160 | 0.802355 | 5.835139e-49 | -8.615850e-47 |
| Msa0701360 | Msa1341840 | 0.822036 | 2.936074e-53 | -8.615850e-47 |
| Msa0701360 | Msa1345100 | 0.822136 | 2.784058e-53 | -8.615850e-47 |
| Msa0701360 | Msa1348090 | 0.803947 | 2.732705e-49 | -8.615850e-47 |
| Msa0701360 | Msa1360980 | 0.800434 | 1.443630e-48 | -8.615850e-47 |
| Msa0701360 | Msa1369690 | 0.828723 | 7.670200e-55 | -8.615850e-47 |
| Msa0701360 | Msa1371520 | 0.807065 | 6.062463e-50 | -8.615850e-47 |
| Msa0701360 | Msa1418240 | 0.850794 | 1.348265e-60 | -8.615850e-47 |
| Msa0701360 | Msa1425880 | 0.800115 | 1.677023e-48 | -8.615850e-47 |
| Msa0701360 | Msa1440310 | 0.820075 | 8.307758e-53 | -8.615850e-47 |
| Msa0701360 | Msa1461370 | 0.808253 | 3.390953e-50 | -8.615850e-47 |
| Msa0701360 | Msa1461540 | 0.837310 | 5.605431e-57 | -8.615850e-47 |
| Msa0138360 | Msa0701360 | 0.822587 | 2.186950e-53 | -8.615850e-47 |
| Msa0147980 | Msa0701360 | 0.800286 | 1.547432e-48 | -8.615850e-47 |
| Msa0170210 | Msa0701360 | 0.818585 | 1.815010e-52 | -8.615850e-47 |
| Msa0183760 | Msa0701360 | 0.801423 | 9.069595e-49 | -8.615850e-47 |
| Msa0207350 | Msa0701360 | 0.820076 | 8.303687e-53 | -8.615850e-47 |
| Msa0209140 | Msa0701360 | 0.816703 | 4.824205e-52 | -8.615850e-47 |
| Msa0214550 | Msa0701360 | 0.818157 | 2.269295e-52 | -8.615850e-47 |
| Msa0237210 | Msa0701360 | 0.812908 | 3.345781e-51 | -8.615850e-47 |
| Msa0378720 | Msa0701360 | 0.831541 | 1.574263e-55 | -8.615850e-47 |
| Msa0382370 | Msa0701360 | 0.808123 | 3.614964e-50 | -8.615850e-47 |
| Msa0403640 | Msa0701360 | 0.806287 | 8.850915e-50 | -8.615850e-47 |
| Msa0427100 | Msa0701360 | 0.808659 | 2.777971e-50 | -8.615850e-47 |
| Msa0427120 | Msa0701360 | 0.813614 | 2.341576e-51 | -8.615850e-47 |
| Msa0428370 | Msa0701360 | 0.813601 | 2.356657e-51 | -8.615850e-47 |
| Msa0432160 | Msa0701360 | 0.813067 | 3.088408e-51 | -8.615850e-47 |
| Msa0450400 | Msa0701360 | -0.850933 | 1.232112e-60 | -8.615850e-47 |
| Msa0460590 | Msa0701360 | 0.800759 | 1.239625e-48 | -8.615850e-47 |
| Msa0467100 | Msa0701360 | 0.815176 | 1.057391e-51 | -8.615850e-47 |
| Msa0468410 | Msa0701360 | 0.818214 | 2.203427e-52 | -8.615850e-47 |
| Msa0474730 | Msa0701360 | 0.826097 | 3.269917e-54 | -8.615850e-47 |
| Msa0476680 | Msa0701360 | 0.815561 | 8.681829e-52 | -8.615850e-47 |
| Msa0479200 | Msa0701360 | 0.801524 | 8.645186e-49 | -8.615850e-47 |
| Msa0481970 | Msa0701360 | 0.823912 | 1.072800e-53 | -8.615850e-47 |
| Msa0240900 | Msa0701360 | 0.850973 | 1.200564e-60 | -8.615850e-47 |
| Msa0241830 | Msa0701360 | 0.820762 | 5.778202e-53 | -8.615850e-47 |
| Msa0261170 | Msa0701360 | 0.810221 | 1.284140e-50 | -8.615850e-47 |
| Msa0271770 | Msa0701360 | 0.804094 | 2.547725e-49 | -8.615850e-47 |
| Msa0279850 | Msa0701360 | 0.838054 | 3.611066e-57 | -8.615850e-47 |
| Msa0281530 | Msa0701360 | 0.801687 | 8.004110e-49 | -8.615850e-47 |
| Msa0283330 | Msa0701360 | 0.803273 | 3.771168e-49 | -8.615850e-47 |
| Msa0298410 | Msa0701360 | 0.812329 | 4.478087e-51 | -8.615850e-47 |
| Msa0300320 | Msa0701360 | 0.829857 | 4.070663e-55 | -8.615850e-47 |
| Msa0315690 | Msa0701360 | 0.811124 | 8.189218e-51 | -8.615850e-47 |
| Msa0317970 | Msa0701360 | 0.868453 | 6.456177e-66 | -8.615850e-47 |
| Msa0322900 | Msa0701360 | 0.829279 | 5.625826e-55 | -8.615850e-47 |
| Msa0341690 | Msa0701360 | 0.825628 | 4.224894e-54 | -8.615850e-47 |
| Msa0485240 | Msa0701360 | 0.851103 | 1.103563e-60 | -8.615850e-47 |
| Msa0518030 | Msa0701360 | 0.822338 | 2.499478e-53 | -8.615850e-47 |
| Msa0521010 | Msa0701360 | 0.814058 | 1.869134e-51 | -8.615850e-47 |
| Msa0527190 | Msa0701360 | 0.813219 | 2.859125e-51 | -8.615850e-47 |
| Msa0556010 | Msa0701360 | 0.817427 | 3.316759e-52 | -8.615850e-47 |
| Msa0565170 | Msa0701360 | 0.883765 | 3.401779e-71 | -8.615850e-47 |
| Msa0582480 | Msa0701360 | 0.828708 | 7.737272e-55 | -8.615850e-47 |
| Msa0583360 | Msa0701360 | -0.829486 | 5.010680e-55 | -8.615850e-47 |
| Msa0586220 | Msa0701360 | 0.817771 | 2.774460e-52 | -8.615850e-47 |
| Msa0590640 | Msa0701360 | 0.809989 | 1.440308e-50 | -8.615850e-47 |
| Msa0601950 | Msa0701360 | 0.825554 | 4.400474e-54 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0701360 | MtrunA17_Chr8g0379551 | 97.639 | 466 | 11 | 0 | 1 | 466 | 14 | 479 | 0.0 | 943 |
| Msa0701360 | MtrunA17_Chr2g0315531 | 44.766 | 449 | 237 | 5 | 14 | 458 | 9 | 450 | 3.25e-122 | 365 |
| Msa0701360 | MtrunA17_Chr8g0359791 | 48.320 | 387 | 185 | 6 | 78 | 459 | 33 | 409 | 3.14e-117 | 350 |
| Msa0701360 | MtrunA17_Chr4g0011611 | 38.750 | 400 | 215 | 5 | 74 | 459 | 30 | 413 | 4.69e-94 | 290 |
| Msa0701360 | MtrunA17_Chr7g0230481 | 33.414 | 413 | 246 | 6 | 62 | 459 | 125 | 523 | 2.60e-63 | 214 |
| Msa0701360 | MtrunA17_Chr3g0103691 | 28.715 | 397 | 256 | 5 | 79 | 458 | 91 | 477 | 5.44e-50 | 177 |
| Msa0701360 | MtrunA17_Chr5g0432511 | 31.099 | 373 | 231 | 5 | 93 | 465 | 33 | 379 | 1.05e-47 | 169 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0701360 | AT1G29300.1 | 54.054 | 481 | 184 | 9 | 1 | 466 | 1 | 459 | 2.39e-169 | 484 |
| Msa0701360 | AT3G14870.1 | 45.064 | 466 | 245 | 7 | 1 | 459 | 5 | 466 | 4.97e-132 | 390 |
| Msa0701360 | AT3G14870.4 | 45.064 | 466 | 245 | 7 | 1 | 459 | 1 | 462 | 5.20e-132 | 390 |
| Msa0701360 | AT3G14870.5 | 45.064 | 466 | 245 | 7 | 1 | 459 | 1 | 462 | 5.20e-132 | 390 |
| Msa0701360 | AT3G14870.2 | 45.064 | 466 | 245 | 7 | 1 | 459 | 1 | 462 | 5.20e-132 | 390 |
| Msa0701360 | AT3G14870.3 | 45.064 | 466 | 245 | 7 | 1 | 459 | 2 | 463 | 5.43e-132 | 390 |
| Msa0701360 | AT1G53380.2 | 44.017 | 468 | 238 | 5 | 1 | 460 | 1 | 452 | 3.26e-130 | 385 |
| Msa0701360 | AT1G53380.4 | 44.017 | 468 | 238 | 5 | 1 | 460 | 1 | 452 | 3.26e-130 | 385 |
| Msa0701360 | AT1G53380.1 | 44.017 | 468 | 238 | 5 | 1 | 460 | 1 | 452 | 3.26e-130 | 385 |
| Msa0701360 | AT1G53380.3 | 44.017 | 468 | 238 | 5 | 1 | 460 | 1 | 452 | 3.26e-130 | 385 |
| Msa0701360 | AT1G53380.5 | 44.017 | 468 | 238 | 5 | 1 | 460 | 1 | 452 | 3.26e-130 | 385 |
| Msa0701360 | AT2G45260.1 | 40.100 | 399 | 220 | 4 | 74 | 461 | 31 | 421 | 1.01e-96 | 298 |
| Msa0701360 | AT3G60680.1 | 34.367 | 387 | 234 | 5 | 81 | 459 | 120 | 494 | 4.21e-72 | 236 |
| Msa0701360 | AT2G30380.1 | 32.663 | 398 | 217 | 8 | 67 | 459 | 60 | 411 | 1.14e-55 | 191 |
| Msa0701360 | AT4G34080.1 | 36.364 | 275 | 164 | 3 | 74 | 337 | 7 | 281 | 1.62e-53 | 181 |
| Msa0701360 | AT4G33320.1 | 35.448 | 268 | 161 | 4 | 74 | 329 | 7 | 274 | 3.28e-48 | 167 |
| Msa0701360 | AT5G58960.2 | 29.557 | 406 | 250 | 7 | 79 | 458 | 85 | 480 | 5.81e-44 | 161 |
| Msa0701360 | AT5G58960.3 | 29.557 | 406 | 250 | 7 | 79 | 458 | 85 | 480 | 5.81e-44 | 161 |
| Msa0701360 | AT5G58960.1 | 29.557 | 406 | 250 | 7 | 79 | 458 | 160 | 555 | 1.79e-43 | 161 |
| Msa0701360 | AT2G32130.1 | 57.724 | 123 | 52 | 0 | 80 | 202 | 34 | 156 | 6.75e-43 | 149 |
| Msa0701360 | AT4G36100.1 | 43.636 | 110 | 57 | 2 | 74 | 178 | 7 | 116 | 7.13e-21 | 91.7 |
Find 58 sgRNAs with CRISPR-Local
Find 154 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCTTGTTCATTGCTGATTT+TGG | 0.079747 | 4_4:+72791804 | None:intergenic |
| TTCTAATTCCGGCGAGTTTC+GGG | 0.120775 | 4_4:-72790533 | Msa0701360:CDS |
| ATGGACAACTTGATGATATT+TGG | 0.163563 | 4_4:+72790813 | None:intergenic |
| ATTCTAATTCCGGCGAGTTT+CGG | 0.208130 | 4_4:-72790534 | Msa0701360:CDS |
| TCATTTGAAACAGTGAAATT+TGG | 0.218352 | 4_4:+72790955 | None:intergenic |
| ATTTGTCAAAATAGTGATTT+TGG | 0.234573 | 4_4:+72790914 | None:intergenic |
| GATTCTATTGATTCTAATTC+CGG | 0.300156 | 4_4:-72790544 | Msa0701360:CDS |
| CAGAGGAAGTTAGTGAATTC+TGG | 0.306974 | 4_4:-72790754 | Msa0701360:CDS |
| TTCTCCTTGAAGCTTCTTGA+TGG | 0.321429 | 4_4:+72791329 | None:intergenic |
| CTTCTTGATGGTGATTTCAT+AGG | 0.327494 | 4_4:+72791341 | None:intergenic |
| TTCTTATTGATCTCAAAGTA+TGG | 0.329293 | 4_4:+72791139 | None:intergenic |
| GAGTCAGGTTTATCTGTCTT+TGG | 0.330222 | 4_4:-72790461 | Msa0701360:CDS |
| CTGAGATGGCAAAGCGTGTT+TGG | 0.331303 | 4_4:-72790696 | Msa0701360:CDS |
| TCAAGTTGTCCATGCTAAAA+TGG | 0.340049 | 4_4:-72790803 | Msa0701360:CDS |
| GAAACTTATGATTGAAGAAA+TGG | 0.349019 | 4_4:-72791109 | Msa0701360:CDS |
| TCAGTTTCAACTTCATGTTC+AGG | 0.353607 | 4_4:-72790568 | Msa0701360:CDS |
| AAAATGGAGTGTTCTTTGTT+TGG | 0.368125 | 4_4:-72790787 | Msa0701360:CDS |
| AAGAAATGGAATCAGCAAAC+TGG | 0.369176 | 4_4:-72791095 | Msa0701360:CDS |
| GATTCAACATTGAAAGTTCA+AGG | 0.402308 | 4_4:+72791187 | None:intergenic |
| AACTTGGCAAAAGATGAGTT+TGG | 0.414833 | 4_4:+72790844 | None:intergenic |
| TTCTAACCTTGTTCTTCTGT+TGG | 0.416097 | 4_4:+72791619 | None:intergenic |
| GGTTAGAAACAAAGCAGTTA+TGG | 0.418035 | 4_4:-72791604 | Msa0701360:CDS |
| CAGAATTCACTAACTTCCTC+TGG | 0.439916 | 4_4:+72790755 | None:intergenic |
| TGTTTGGACACTGCATTACT+TGG | 0.442751 | 4_4:-72790680 | Msa0701360:CDS |
| AAAACAACGATGACTTGGTT+TGG | 0.448235 | 4_4:+72791026 | None:intergenic |
| ATCATATGCAGAACTTCAAA+TGG | 0.451722 | 4_4:-72791535 | Msa0701360:CDS |
| TAGGTTCTCTGAGGTTTACA+TGG | 0.451972 | 4_4:-72790608 | Msa0701360:CDS |
| ATCACCATCAAGAAGCTTCA+AGG | 0.452355 | 4_4:-72791333 | Msa0701360:CDS |
| CAGTTTCAACTTCATGTTCA+GGG | 0.460825 | 4_4:-72790567 | Msa0701360:CDS |
| TGTATTGAATCATTGTTGTA+AGG | 0.470099 | 4_4:+72791504 | None:intergenic |
| TAAGACAGTGATACAGAGTC+AGG | 0.472174 | 4_4:-72790476 | Msa0701360:CDS |
| TCAGGTTTATCTGTCTTTGG+TGG | 0.472612 | 4_4:-72790458 | Msa0701360:CDS |
| TTCAGGTCAAGAAGAATACT+AGG | 0.473719 | 4_4:-72790627 | Msa0701360:CDS |
| GGTTTGGTGAAAACTGCGTT+TGG | 0.476936 | 4_4:+72791042 | None:intergenic |
| CCATCTCAGAATTGAAAAGA+AGG | 0.510329 | 4_4:-72791446 | Msa0701360:CDS |
| AAAGCAAAACAACGATGACT+TGG | 0.515541 | 4_4:+72791021 | None:intergenic |
| AGAAATGGAATCAGCAAACT+GGG | 0.539408 | 4_4:-72791094 | Msa0701360:CDS |
| TTTGGTGAATATTGTGAAGA+GGG | 0.541008 | 4_4:+72790932 | None:intergenic |
| CAAAATCAGCAATGAACAAG+AGG | 0.553083 | 4_4:-72791803 | Msa0701360:CDS |
| TGGTGAAAACTGCGTTTGGA+TGG | 0.556904 | 4_4:+72791046 | None:intergenic |
| GTGGTGCCTGGTTTCAACAT+TGG | 0.564074 | 4_4:-72790499 | Msa0701360:CDS |
| TAATTCCGGCGAGTTTCGGG+TGG | 0.572386 | 4_4:-72790530 | Msa0701360:CDS |
| AGCAGCTGATCAAGCAGTGG+TGG | 0.584305 | 4_4:-72791481 | Msa0701360:CDS |
| GAAAACCACCCGAAACTCGC+CGG | 0.587240 | 4_4:+72790525 | None:intergenic |
| ATTTGATGGTGATCTGTGAA+AGG | 0.594388 | 4_4:+72791660 | None:intergenic |
| AGTTTCAACTTCATGTTCAG+GGG | 0.594405 | 4_4:-72790566 | Msa0701360:CDS |
| CAACTCACATCTCAAAGTGA+AGG | 0.600233 | 4_4:-72791694 | Msa0701360:CDS |
| TGTTTGGAAATTTGAACCAG+AGG | 0.621071 | 4_4:-72790771 | Msa0701360:CDS |
| ATTTGCAAAGACATTCCAGA+AGG | 0.624146 | 4_4:-72791772 | Msa0701360:CDS |
| TTATGGAGGCTCTAATAGCA+AGG | 0.629498 | 4_4:-72791587 | Msa0701360:CDS |
| AGGAAGTTAGTGAATTCTGG+AGG | 0.632480 | 4_4:-72790751 | Msa0701360:CDS |
| GCTAGCATTATAGTAACTTG+AGG | 0.633614 | 4_4:+72791396 | None:intergenic |
| GTCTTACCAATGTTGAAACC+AGG | 0.633731 | 4_4:+72790493 | None:intergenic |
| TAGAAACAAAGCAGTTATGG+AGG | 0.646650 | 4_4:-72791601 | Msa0701360:CDS |
| GGAAGTTAGTGAATTCTGGA+GGG | 0.667121 | 4_4:-72790750 | Msa0701360:CDS |
| GAAGAATACTAGGTTCTCTG+AGG | 0.672182 | 4_4:-72790617 | Msa0701360:CDS |
| ACAAGCAGCTGATCAAGCAG+TGG | 0.679340 | 4_4:-72791484 | Msa0701360:CDS |
| TAAGCACCAACAGAAGAACA+AGG | 0.710444 | 4_4:-72791625 | Msa0701360:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAAAAATTGCTTCAAATAA+TGG | - | chr4_4:72790528-72790547 | Msa0701360:CDS | 20.0% |
| !!! | TACTATTAGTTTTCATTTGA+TGG | + | chr4_4:72790614-72790633 | None:intergenic | 20.0% |
| !!! | ATTTGTCAAAATAGTGATTT+TGG | + | chr4_4:72791346-72791365 | None:intergenic | 20.0% |
| !!! | ATATTTGGATTTTAGAAACT+TGG | + | chr4_4:72791432-72791451 | None:intergenic | 20.0% |
| !! | ACAAAAATTGCTTCAAATAA+TGG | - | chr4_4:72790528-72790547 | Msa0701360:CDS | 20.0% |
| !!! | TACTATTAGTTTTCATTTGA+TGG | + | chr4_4:72790614-72790633 | None:intergenic | 20.0% |
| !!! | ATTTGTCAAAATAGTGATTT+TGG | + | chr4_4:72791346-72791365 | None:intergenic | 20.0% |
| !!! | ATATTTGGATTTTAGAAACT+TGG | + | chr4_4:72791432-72791451 | None:intergenic | 20.0% |
| ! | TGTATTGAATCATTGTTGTA+AGG | + | chr4_4:72790756-72790775 | None:intergenic | 25.0% |
| ! | TTCTTATTGATCTCAAAGTA+TGG | + | chr4_4:72791121-72791140 | None:intergenic | 25.0% |
| ! | GAAACTTATGATTGAAGAAA+TGG | - | chr4_4:72791148-72791167 | Msa0701360:CDS | 25.0% |
| !! | TCATTTGAAACAGTGAAATT+TGG | + | chr4_4:72791305-72791324 | None:intergenic | 25.0% |
| !!! | ACTATTTTGACAAATTCAAG+AGG | - | chr4_4:72791351-72791370 | Msa0701360:CDS | 25.0% |
| !!! | TTGTTTTCTAAGTACTGTTT+TGG | + | chr4_4:72791392-72791411 | None:intergenic | 25.0% |
| !!! | GATTCTATTGATTCTAATTC+CGG | - | chr4_4:72791713-72791732 | Msa0701360:CDS | 25.0% |
| ! | TGTATTGAATCATTGTTGTA+AGG | + | chr4_4:72790756-72790775 | None:intergenic | 25.0% |
| ! | TTCTTATTGATCTCAAAGTA+TGG | + | chr4_4:72791121-72791140 | None:intergenic | 25.0% |
| ! | GAAACTTATGATTGAAGAAA+TGG | - | chr4_4:72791148-72791167 | Msa0701360:CDS | 25.0% |
| !! | TCATTTGAAACAGTGAAATT+TGG | + | chr4_4:72791305-72791324 | None:intergenic | 25.0% |
| !!! | ACTATTTTGACAAATTCAAG+AGG | - | chr4_4:72791351-72791370 | Msa0701360:CDS | 25.0% |
| !!! | TTGTTTTCTAAGTACTGTTT+TGG | + | chr4_4:72791392-72791411 | None:intergenic | 25.0% |
| !!! | GATTCTATTGATTCTAATTC+CGG | - | chr4_4:72791713-72791732 | Msa0701360:CDS | 25.0% |
| ! | TTCTGCATATGATGCTTTTA+TGG | + | chr4_4:72790715-72790734 | None:intergenic | 30.0% |
| ATCATATGCAGAACTTCAAA+TGG | - | chr4_4:72790722-72790741 | Msa0701360:CDS | 30.0% | |
| !!! | CTCATCAAGTTTTTTTCTGA+GGG | + | chr4_4:72790982-72791001 | None:intergenic | 30.0% |
| !!! | ACTCATCAAGTTTTTTTCTG+AGG | + | chr4_4:72790983-72791002 | None:intergenic | 30.0% |
| GATTCAACATTGAAAGTTCA+AGG | + | chr4_4:72791073-72791092 | None:intergenic | 30.0% | |
| TTTGGTGAATATTGTGAAGA+GGG | + | chr4_4:72791328-72791347 | None:intergenic | 30.0% | |
| ! | TTTTGGTGAATATTGTGAAG+AGG | + | chr4_4:72791329-72791348 | None:intergenic | 30.0% |
| ! | ATGGACAACTTGATGATATT+TGG | + | chr4_4:72791447-72791466 | None:intergenic | 30.0% |
| ! | AAAATGGAGTGTTCTTTGTT+TGG | - | chr4_4:72791470-72791489 | Msa0701360:CDS | 30.0% |
| ! | AGAAGATGTTAGCATTTTTC+AGG | - | chr4_4:72791613-72791632 | Msa0701360:CDS | 30.0% |
| ! | TTCTGCATATGATGCTTTTA+TGG | + | chr4_4:72790715-72790734 | None:intergenic | 30.0% |
| ATCATATGCAGAACTTCAAA+TGG | - | chr4_4:72790722-72790741 | Msa0701360:CDS | 30.0% | |
| !!! | CTCATCAAGTTTTTTTCTGA+GGG | + | chr4_4:72790982-72791001 | None:intergenic | 30.0% |
| !!! | ACTCATCAAGTTTTTTTCTG+AGG | + | chr4_4:72790983-72791002 | None:intergenic | 30.0% |
| GATTCAACATTGAAAGTTCA+AGG | + | chr4_4:72791073-72791092 | None:intergenic | 30.0% | |
| TTTGGTGAATATTGTGAAGA+GGG | + | chr4_4:72791328-72791347 | None:intergenic | 30.0% | |
| ! | TTTTGGTGAATATTGTGAAG+AGG | + | chr4_4:72791329-72791348 | None:intergenic | 30.0% |
| ! | ATGGACAACTTGATGATATT+TGG | + | chr4_4:72791447-72791466 | None:intergenic | 30.0% |
| ! | AAAATGGAGTGTTCTTTGTT+TGG | - | chr4_4:72791470-72791489 | Msa0701360:CDS | 30.0% |
| ! | AGAAGATGTTAGCATTTTTC+AGG | - | chr4_4:72791613-72791632 | Msa0701360:CDS | 30.0% |
| !!! | CTCTTGTTCATTGCTGATTT+TGG | + | chr4_4:72790456-72790475 | None:intergenic | 35.0% |
| CAAAATCAGCAATGAACAAG+AGG | - | chr4_4:72790454-72790473 | Msa0701360:CDS | 35.0% | |
| ATTTGCAAAGACATTCCAGA+AGG | - | chr4_4:72790485-72790504 | Msa0701360:CDS | 35.0% | |
| !!! | TTTTGAGACTAATCACCTTC+TGG | + | chr4_4:72790503-72790522 | None:intergenic | 35.0% |
| !! | ATTTGATGGTGATCTGTGAA+AGG | + | chr4_4:72790600-72790619 | None:intergenic | 35.0% |
| TTCTAACCTTGTTCTTCTGT+TGG | + | chr4_4:72790641-72790660 | None:intergenic | 35.0% | |
| GGTTAGAAACAAAGCAGTTA+TGG | - | chr4_4:72790653-72790672 | Msa0701360:CDS | 35.0% | |
| TAGAAACAAAGCAGTTATGG+AGG | - | chr4_4:72790656-72790675 | Msa0701360:CDS | 35.0% | |
| !! | CCTTCTTTTCAATTCTGAGA+TGG | + | chr4_4:72790814-72790833 | None:intergenic | 35.0% |
| CCATCTCAGAATTGAAAAGA+AGG | - | chr4_4:72790811-72790830 | Msa0701360:CDS | 35.0% | |
| GCTAGCATTATAGTAACTTG+AGG | + | chr4_4:72790864-72790883 | None:intergenic | 35.0% | |
| !! | CTTCTTGATGGTGATTTCAT+AGG | + | chr4_4:72790919-72790938 | None:intergenic | 35.0% |
| AAGAAATGGAATCAGCAAAC+TGG | - | chr4_4:72791162-72791181 | Msa0701360:CDS | 35.0% | |
| AGAAATGGAATCAGCAAACT+GGG | - | chr4_4:72791163-72791182 | Msa0701360:CDS | 35.0% | |
| !! | AAAACAACGATGACTTGGTT+TGG | + | chr4_4:72791234-72791253 | None:intergenic | 35.0% |
| AAAGCAAAACAACGATGACT+TGG | + | chr4_4:72791239-72791258 | None:intergenic | 35.0% | |
| AACTTGGCAAAAGATGAGTT+TGG | + | chr4_4:72791416-72791435 | None:intergenic | 35.0% | |
| ! | TCAAGTTGTCCATGCTAAAA+TGG | - | chr4_4:72791454-72791473 | Msa0701360:CDS | 35.0% |
| !! | AAGAACACTCCATTTTAGCA+TGG | + | chr4_4:72791466-72791485 | None:intergenic | 35.0% |
| TGTTTGGAAATTTGAACCAG+AGG | - | chr4_4:72791486-72791505 | Msa0701360:CDS | 35.0% | |
| GCTAAGAAAAAAGCTGAATC+TGG | + | chr4_4:72791536-72791555 | None:intergenic | 35.0% | |
| !! | TTTCTTAGCTTTTGCTGAGA+TGG | - | chr4_4:72791547-72791566 | Msa0701360:CDS | 35.0% |
| TTCAGGTCAAGAAGAATACT+AGG | - | chr4_4:72791630-72791649 | Msa0701360:CDS | 35.0% | |
| TCAGTTTCAACTTCATGTTC+AGG | - | chr4_4:72791689-72791708 | Msa0701360:CDS | 35.0% | |
| CAGTTTCAACTTCATGTTCA+GGG | - | chr4_4:72791690-72791709 | Msa0701360:CDS | 35.0% | |
| AGTTTCAACTTCATGTTCAG+GGG | - | chr4_4:72791691-72791710 | Msa0701360:CDS | 35.0% | |
| !!! | CTCTTGTTCATTGCTGATTT+TGG | + | chr4_4:72790456-72790475 | None:intergenic | 35.0% |
| CAAAATCAGCAATGAACAAG+AGG | - | chr4_4:72790454-72790473 | Msa0701360:CDS | 35.0% | |
| ATTTGCAAAGACATTCCAGA+AGG | - | chr4_4:72790485-72790504 | Msa0701360:CDS | 35.0% | |
| !!! | TTTTGAGACTAATCACCTTC+TGG | + | chr4_4:72790503-72790522 | None:intergenic | 35.0% |
| !! | ATTTGATGGTGATCTGTGAA+AGG | + | chr4_4:72790600-72790619 | None:intergenic | 35.0% |
| TTCTAACCTTGTTCTTCTGT+TGG | + | chr4_4:72790641-72790660 | None:intergenic | 35.0% | |
| GGTTAGAAACAAAGCAGTTA+TGG | - | chr4_4:72790653-72790672 | Msa0701360:CDS | 35.0% | |
| TAGAAACAAAGCAGTTATGG+AGG | - | chr4_4:72790656-72790675 | Msa0701360:CDS | 35.0% | |
| !! | CCTTCTTTTCAATTCTGAGA+TGG | + | chr4_4:72790814-72790833 | None:intergenic | 35.0% |
| CCATCTCAGAATTGAAAAGA+AGG | - | chr4_4:72790811-72790830 | Msa0701360:CDS | 35.0% | |
| GCTAGCATTATAGTAACTTG+AGG | + | chr4_4:72790864-72790883 | None:intergenic | 35.0% | |
| !! | CTTCTTGATGGTGATTTCAT+AGG | + | chr4_4:72790919-72790938 | None:intergenic | 35.0% |
| AAGAAATGGAATCAGCAAAC+TGG | - | chr4_4:72791162-72791181 | Msa0701360:CDS | 35.0% | |
| AGAAATGGAATCAGCAAACT+GGG | - | chr4_4:72791163-72791182 | Msa0701360:CDS | 35.0% | |
| !! | AAAACAACGATGACTTGGTT+TGG | + | chr4_4:72791234-72791253 | None:intergenic | 35.0% |
| AAAGCAAAACAACGATGACT+TGG | + | chr4_4:72791239-72791258 | None:intergenic | 35.0% | |
| AACTTGGCAAAAGATGAGTT+TGG | + | chr4_4:72791416-72791435 | None:intergenic | 35.0% | |
| ! | TCAAGTTGTCCATGCTAAAA+TGG | - | chr4_4:72791454-72791473 | Msa0701360:CDS | 35.0% |
| !! | AAGAACACTCCATTTTAGCA+TGG | + | chr4_4:72791466-72791485 | None:intergenic | 35.0% |
| TGTTTGGAAATTTGAACCAG+AGG | - | chr4_4:72791486-72791505 | Msa0701360:CDS | 35.0% | |
| GCTAAGAAAAAAGCTGAATC+TGG | + | chr4_4:72791536-72791555 | None:intergenic | 35.0% | |
| !! | TTTCTTAGCTTTTGCTGAGA+TGG | - | chr4_4:72791547-72791566 | Msa0701360:CDS | 35.0% |
| TTCAGGTCAAGAAGAATACT+AGG | - | chr4_4:72791630-72791649 | Msa0701360:CDS | 35.0% | |
| TCAGTTTCAACTTCATGTTC+AGG | - | chr4_4:72791689-72791708 | Msa0701360:CDS | 35.0% | |
| CAGTTTCAACTTCATGTTCA+GGG | - | chr4_4:72791690-72791709 | Msa0701360:CDS | 35.0% | |
| AGTTTCAACTTCATGTTCAG+GGG | - | chr4_4:72791691-72791710 | Msa0701360:CDS | 35.0% | |
| CAACTCACATCTCAAAGTGA+AGG | - | chr4_4:72790563-72790582 | Msa0701360:CDS | 40.0% | |
| TAAGCACCAACAGAAGAACA+AGG | - | chr4_4:72790632-72790651 | Msa0701360:CDS | 40.0% | |
| ! | TTATGGAGGCTCTAATAGCA+AGG | - | chr4_4:72790670-72790689 | Msa0701360:CDS | 40.0% |
| ! | CTAATAGCAAGGCTTTTTGC+TGG | - | chr4_4:72790681-72790700 | Msa0701360:CDS | 40.0% |
| ATCACCATCAAGAAGCTTCA+AGG | - | chr4_4:72790924-72790943 | Msa0701360:CDS | 40.0% | |
| TTCTCCTTGAAGCTTCTTGA+TGG | + | chr4_4:72790931-72790950 | None:intergenic | 40.0% | |
| CAGAATTCACTAACTTCCTC+TGG | + | chr4_4:72791505-72791524 | None:intergenic | 40.0% | |
| CAGAGGAAGTTAGTGAATTC+TGG | - | chr4_4:72791503-72791522 | Msa0701360:CDS | 40.0% | |
| AGGAAGTTAGTGAATTCTGG+AGG | - | chr4_4:72791506-72791525 | Msa0701360:CDS | 40.0% | |
| GGAAGTTAGTGAATTCTGGA+GGG | - | chr4_4:72791507-72791526 | Msa0701360:CDS | 40.0% | |
| TGTTTGGACACTGCATTACT+TGG | - | chr4_4:72791577-72791596 | Msa0701360:CDS | 40.0% | |
| GAAGAATACTAGGTTCTCTG+AGG | - | chr4_4:72791640-72791659 | Msa0701360:CDS | 40.0% | |
| !! | TAGGTTCTCTGAGGTTTACA+TGG | - | chr4_4:72791649-72791668 | Msa0701360:CDS | 40.0% |
| ATTCTAATTCCGGCGAGTTT+CGG | - | chr4_4:72791723-72791742 | Msa0701360:CDS | 40.0% | |
| GTCTTACCAATGTTGAAACC+AGG | + | chr4_4:72791767-72791786 | None:intergenic | 40.0% | |
| TAAGACAGTGATACAGAGTC+AGG | - | chr4_4:72791781-72791800 | Msa0701360:CDS | 40.0% | |
| GAGTCAGGTTTATCTGTCTT+TGG | - | chr4_4:72791796-72791815 | Msa0701360:CDS | 40.0% | |
| TCAGGTTTATCTGTCTTTGG+TGG | - | chr4_4:72791799-72791818 | Msa0701360:CDS | 40.0% | |
| CAACTCACATCTCAAAGTGA+AGG | - | chr4_4:72790563-72790582 | Msa0701360:CDS | 40.0% | |
| TAAGCACCAACAGAAGAACA+AGG | - | chr4_4:72790632-72790651 | Msa0701360:CDS | 40.0% | |
| ! | TTATGGAGGCTCTAATAGCA+AGG | - | chr4_4:72790670-72790689 | Msa0701360:CDS | 40.0% |
| ! | CTAATAGCAAGGCTTTTTGC+TGG | - | chr4_4:72790681-72790700 | Msa0701360:CDS | 40.0% |
| ATCACCATCAAGAAGCTTCA+AGG | - | chr4_4:72790924-72790943 | Msa0701360:CDS | 40.0% | |
| TTCTCCTTGAAGCTTCTTGA+TGG | + | chr4_4:72790931-72790950 | None:intergenic | 40.0% | |
| CAGAATTCACTAACTTCCTC+TGG | + | chr4_4:72791505-72791524 | None:intergenic | 40.0% | |
| CAGAGGAAGTTAGTGAATTC+TGG | - | chr4_4:72791503-72791522 | Msa0701360:CDS | 40.0% | |
| AGGAAGTTAGTGAATTCTGG+AGG | - | chr4_4:72791506-72791525 | Msa0701360:CDS | 40.0% | |
| GGAAGTTAGTGAATTCTGGA+GGG | - | chr4_4:72791507-72791526 | Msa0701360:CDS | 40.0% | |
| TGTTTGGACACTGCATTACT+TGG | - | chr4_4:72791577-72791596 | Msa0701360:CDS | 40.0% | |
| GAAGAATACTAGGTTCTCTG+AGG | - | chr4_4:72791640-72791659 | Msa0701360:CDS | 40.0% | |
| !! | TAGGTTCTCTGAGGTTTACA+TGG | - | chr4_4:72791649-72791668 | Msa0701360:CDS | 40.0% |
| ATTCTAATTCCGGCGAGTTT+CGG | - | chr4_4:72791723-72791742 | Msa0701360:CDS | 40.0% | |
| GTCTTACCAATGTTGAAACC+AGG | + | chr4_4:72791767-72791786 | None:intergenic | 40.0% | |
| TAAGACAGTGATACAGAGTC+AGG | - | chr4_4:72791781-72791800 | Msa0701360:CDS | 40.0% | |
| GAGTCAGGTTTATCTGTCTT+TGG | - | chr4_4:72791796-72791815 | Msa0701360:CDS | 40.0% | |
| TCAGGTTTATCTGTCTTTGG+TGG | - | chr4_4:72791799-72791818 | Msa0701360:CDS | 40.0% | |
| TGGTGAAAACTGCGTTTGGA+TGG | + | chr4_4:72791214-72791233 | None:intergenic | 45.0% | |
| !! | GGTTTGGTGAAAACTGCGTT+TGG | + | chr4_4:72791218-72791237 | None:intergenic | 45.0% |
| TTCTAATTCCGGCGAGTTTC+GGG | - | chr4_4:72791724-72791743 | Msa0701360:CDS | 45.0% | |
| TGGTGAAAACTGCGTTTGGA+TGG | + | chr4_4:72791214-72791233 | None:intergenic | 45.0% | |
| !! | GGTTTGGTGAAAACTGCGTT+TGG | + | chr4_4:72791218-72791237 | None:intergenic | 45.0% |
| TTCTAATTCCGGCGAGTTTC+GGG | - | chr4_4:72791724-72791743 | Msa0701360:CDS | 45.0% | |
| ACAAGCAGCTGATCAAGCAG+TGG | - | chr4_4:72790773-72790792 | Msa0701360:CDS | 50.0% | |
| CTGAGATGGCAAAGCGTGTT+TGG | - | chr4_4:72791561-72791580 | Msa0701360:CDS | 50.0% | |
| !! | CGAGTTTCGGGTGGTTTTCA+CGG | - | chr4_4:72791736-72791755 | Msa0701360:CDS | 50.0% |
| GTGGTGCCTGGTTTCAACAT+TGG | - | chr4_4:72791758-72791777 | Msa0701360:CDS | 50.0% | |
| ACAAGCAGCTGATCAAGCAG+TGG | - | chr4_4:72790773-72790792 | Msa0701360:CDS | 50.0% | |
| CTGAGATGGCAAAGCGTGTT+TGG | - | chr4_4:72791561-72791580 | Msa0701360:CDS | 50.0% | |
| !! | CGAGTTTCGGGTGGTTTTCA+CGG | - | chr4_4:72791736-72791755 | Msa0701360:CDS | 50.0% |
| GTGGTGCCTGGTTTCAACAT+TGG | - | chr4_4:72791758-72791777 | Msa0701360:CDS | 50.0% | |
| AGCAGCTGATCAAGCAGTGG+TGG | - | chr4_4:72790776-72790795 | Msa0701360:CDS | 55.0% | |
| TAATTCCGGCGAGTTTCGGG+TGG | - | chr4_4:72791727-72791746 | Msa0701360:CDS | 55.0% | |
| GAAAACCACCCGAAACTCGC+CGG | + | chr4_4:72791735-72791754 | None:intergenic | 55.0% | |
| !! | GTTTCGGGTGGTTTTCACGG+TGG | - | chr4_4:72791739-72791758 | Msa0701360:CDS | 55.0% |
| AGCAGCTGATCAAGCAGTGG+TGG | - | chr4_4:72790776-72790795 | Msa0701360:CDS | 55.0% | |
| TAATTCCGGCGAGTTTCGGG+TGG | - | chr4_4:72791727-72791746 | Msa0701360:CDS | 55.0% | |
| GAAAACCACCCGAAACTCGC+CGG | + | chr4_4:72791735-72791754 | None:intergenic | 55.0% | |
| !! | GTTTCGGGTGGTTTTCACGG+TGG | - | chr4_4:72791739-72791758 | Msa0701360:CDS | 55.0% |
| ! | GTGGTTTTCACGGTGGTGCC+TGG | - | chr4_4:72791746-72791765 | Msa0701360:CDS | 60.0% |
| ! | GTGGTTTTCACGGTGGTGCC+TGG | - | chr4_4:72791746-72791765 | Msa0701360:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_4 | gene | 72790438 | 72791841 | 72790438 | ID=Msa0701360;Name=Msa0701360 |
| chr4_4 | mRNA | 72790438 | 72791841 | 72790438 | ID=Msa0701360-mRNA-1;Parent=Msa0701360;Name=Msa0701360-mRNA-1;_AED=0.07;_eAED=0.07;_QI=0|-1|0|1|-1|1|1|0|467 |
| chr4_4 | exon | 72790438 | 72791841 | 72790438 | ID=Msa0701360-mRNA-1:exon:22291;Parent=Msa0701360-mRNA-1 |
| chr4_4 | CDS | 72790438 | 72791841 | 72790438 | ID=Msa0701360-mRNA-1:cds;Parent=Msa0701360-mRNA-1 |
| Gene Sequence |
| Protein sequence |
