Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0004540 | Msa0962240 | 0.824335 | 8.535116e-54 | -8.615850e-47 |
| Msa0029260 | Msa0962240 | 0.807707 | 4.432477e-50 | -8.615850e-47 |
| Msa0033880 | Msa0962240 | 0.821463 | 3.983712e-53 | -8.615850e-47 |
| Msa0055100 | Msa0962240 | 0.804298 | 2.309698e-49 | -8.615850e-47 |
| Msa0068020 | Msa0962240 | 0.818039 | 2.413660e-52 | -8.615850e-47 |
| Msa0091610 | Msa0962240 | 0.824584 | 7.458395e-54 | -8.615850e-47 |
| Msa0101180 | Msa0962240 | 0.806442 | 8.208496e-50 | -8.615850e-47 |
| Msa0115370 | Msa0962240 | 0.831817 | 1.346515e-55 | -8.615850e-47 |
| Msa0121820 | Msa0962240 | 0.805381 | 1.372021e-49 | -8.615850e-47 |
| Msa0126880 | Msa0962240 | 0.807297 | 5.415837e-50 | -8.615850e-47 |
Find 14 sgRNAs with CRISPR-Local
Find 380 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGGAAGCTTGTGTTGCA+AGG | 0.579570 | 6_3:+85277542 | Msa0962240:CDS |
| ACTCTTTCATTGATCAGTGG+TGG | 0.511431 | 6_3:-85270290 | None:intergenic |
| ATCAGTGGTGGACCTTCTCT+AGG | 0.554884 | 6_3:-85270278 | None:intergenic |
| CACTGATCAATGAAAGAGTG+AGG | 0.647069 | 6_3:+85270294 | Msa0962240:CDS |
| CTCACTCTTTCATTGATCAG+TGG | 0.595253 | 6_3:-85270293 | None:intergenic |
| GAGCAATGGAATCTACAAAA+AGG | 0.391170 | 6_3:+85277525 | Msa0962240:CDS |
| GCACATCTCTGTCCTAGAGA+AGG | 0.607548 | 6_3:+85270266 | Msa0962240:CDS |
| GCTTCTGATGACGTCATCTT+CGG | 0.465787 | 6_3:-85277588 | None:intergenic |
| GGAAGCGTTCTGAAGACAAA+AGG | 0.529632 | 6_3:+85277563 | Msa0962240:CDS |
| TAGATCAAGCTGTTACAAGA+TGG | 0.552558 | 6_3:-85272755 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAGGATGTGAATTTGGATT+TGG | + | chr6_3:85270517-85270536 | Msa0962240:intron | 30.0% | |
| AAGGATGTGAATTTGGATTT+GGG | + | chr6_3:85270518-85270537 | Msa0962240:intron | 30.0% | |
| ACAACTATCATCAGACAAAA+AGG | - | chr6_3:85270611-85270630 | None:intergenic | 30.0% | |
| TGATGAGATGTTTGAATTTG+AGG | + | chr6_3:85270665-85270684 | Msa0962240:intron | 30.0% | |
| TAGACCTTCCAAGAAATAAA+TGG | - | chr6_3:85270789-85270808 | None:intergenic | 30.0% | |
| TGGACAACTCATTAGAATAT+TGG | - | chr6_3:85270812-85270831 | None:intergenic | 30.0% | |
| TATTCTAATGAGTTGTCCAT+TGG | + | chr6_3:85270813-85270832 | Msa0962240:intron | 30.0% | |
| GTCCAATACTAATAAATCCA+AGG | + | chr6_3:85270871-85270890 | Msa0962240:intron | 30.0% | |
| CCAATACTAATAAATCCAAG+GGG | + | chr6_3:85270873-85270892 | Msa0962240:intron | 30.0% | |
| CATTGAATAAATGACGTATG+TGG | + | chr6_3:85271000-85271019 | Msa0962240:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_3 | gene | 85270263 | 85277628 | 85270263 | ID=Msa0962240;Name=Msa0962240 |
| chr6_3 | mRNA | 85270263 | 85277628 | 85270263 | ID=Msa0962240-mRNA-1;Parent=Msa0962240;Name=Msa0962240-mRNA-1;_AED=0.85;_eAED=0.85;_QI=0|0|0.33|0.33|1|1|3|0|78 |
| chr6_3 | exon | 85270263 | 85270315 | 85270263 | ID=Msa0962240-mRNA-1:exon:15303;Parent=Msa0962240-mRNA-1 |
| chr6_3 | exon | 85272730 | 85272791 | 85272730 | ID=Msa0962240-mRNA-1:exon:15304;Parent=Msa0962240-mRNA-1 |
| chr6_3 | exon | 85277507 | 85277628 | 85277507 | ID=Msa0962240-mRNA-1:exon:15305;Parent=Msa0962240-mRNA-1 |
| chr6_3 | CDS | 85270263 | 85270315 | 85270263 | ID=Msa0962240-mRNA-1:cds;Parent=Msa0962240-mRNA-1 |
| chr6_3 | CDS | 85272730 | 85272791 | 85272730 | ID=Msa0962240-mRNA-1:cds;Parent=Msa0962240-mRNA-1 |
| chr6_3 | CDS | 85277507 | 85277628 | 85277507 | ID=Msa0962240-mRNA-1:cds;Parent=Msa0962240-mRNA-1 |
| Gene Sequence |
>Msa0962240 ATGGCACATCTCTGTCCTAGAGAAGGTCCACCACTGATCAATGAAAGAGTGAGGAGGGATGCAGTGAGTGGTTTCAATCCATCTTGTAACAGCTTGATCTATGTAAGTAGAAGAGGTTCTGAAGAACAAATGAGAGCAATGGAATCTACAAAAAGGAAGCTTGTGTTGCAAGGAAGCGTTCTGAAGACAAAAGGTTCCGAAGATGACGTCATCAGAAGCACTCTCATCAGAAGCTGA |
| Protein sequence |
>Msa0962240 MAHLCPREGPPLINERVRRDAVSGFNPSCNSLIYVSRRGSEEQMRAMESTKRKLVLQGSVLKTKGSEDDVIRSTLIRS* |
