AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene80163

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene80163.t1 MTR_3g105650 96.019 427 17 0 1 427 1 427 0.0 862
MS.gene80163.t1 MTR_8g073260 44.469 461 208 9 1 427 4 450 1.74e-120 358
MS.gene80163.t1 MTR_8g072510 44.802 404 188 5 5 382 10 404 2.82e-113 340
MS.gene80163.t1 MTR_3g016210 42.299 435 200 6 3 395 8 433 8.46e-110 330
MS.gene80163.t1 MTR_8g039620 44.665 403 188 6 2 376 11 406 4.70e-108 326
MS.gene80163.t1 MTR_1g059930 52.273 308 139 2 5 311 12 312 6.79e-105 318
MS.gene80163.t1 MTR_8g062410 44.963 407 182 7 3 382 8 399 1.07e-99 305
MS.gene80163.t1 MTR_8g467780 40.698 430 208 7 5 396 11 431 1.45e-99 305
MS.gene80163.t1 MTR_8g069880 41.646 413 201 7 3 386 5 406 7.93e-99 303
MS.gene80163.t1 MTR_7g451420 49.839 311 146 3 3 311 7 309 2.41e-98 297
MS.gene80163.t1 MTR_7g021090 42.677 396 184 8 3 369 7 388 1.48e-97 298
MS.gene80163.t1 MTR_8g068990 42.932 382 168 8 5 358 12 371 1.48e-90 281
MS.gene80163.t1 MTR_8g066670 40.000 405 208 4 5 383 1075 1470 2.26e-88 293
MS.gene80163.t1 MTR_8g040930 43.175 359 164 6 48 376 2 350 4.02e-86 268
MS.gene80163.t1 MTR_8g041240 43.175 359 164 6 48 376 2 350 4.02e-86 268
MS.gene80163.t1 MTR_8g073275 35.232 474 194 14 48 427 2 456 2.03e-70 229
MS.gene80163.t1 MTR_1g115575 24.289 457 292 12 8 427 138 577 1.29e-29 121
MS.gene80163.t1 MTR_8g105260 25.059 427 265 12 40 426 101 512 2.18e-24 105
MS.gene80163.t1 MTR_7g100430 25.000 440 271 14 38 420 124 561 1.51e-21 97.4
MS.gene80163.t1 MTR_2g101320 28.372 215 143 2 33 246 134 338 5.09e-21 95.9
MS.gene80163.t1 MTR_7g117620 26.688 311 189 7 40 330 119 410 1.13e-20 94.7
MS.gene80163.t1 MTR_2g096190 25.228 329 199 8 8 310 73 380 9.47e-20 92.0
MS.gene80163.t1 MTR_1g069110 21.429 448 253 9 8 402 21 422 1.17e-19 91.3
MS.gene80163.t1 MTR_7g099580 31.694 183 112 4 14 191 21 195 5.96e-19 87.8
MS.gene80163.t1 MTR_2g101310 27.103 214 141 3 36 246 137 338 2.41e-17 84.7
MS.gene80163.t1 MTR_2g101310 26.887 212 144 2 36 246 137 338 3.59e-17 84.0
MS.gene80163.t1 MTR_7g099570 29.319 191 121 3 8 193 12 193 4.66e-17 84.0
MS.gene80163.t1 MTR_4g062100 24.848 330 174 9 3 310 107 384 1.06e-15 79.3
MS.gene80163.t1 MTR_7g100390 23.765 324 209 7 8 310 56 362 1.41e-13 72.8
MS.gene80163.t1 MTR_1g032100 20.771 467 273 11 3 426 93 505 1.95e-13 72.4
MS.gene80163.t1 MTR_5g085500 26.347 167 109 4 67 228 1 158 8.64e-13 70.1
MS.gene80163.t1 MTR_5g085830 24.658 219 153 6 39 254 277 486 2.16e-12 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene80163.t1 AT4G10720 40.098 409 223 6 1 391 1 405 3.26e-90 280
MS.gene80163.t1 AT4G10720 44.409 313 172 2 1 311 1 313 1.76e-84 265
MS.gene80163.t1 AT1G14500 35.922 412 237 6 1 386 1 411 2.40e-79 252
MS.gene80163.t1 AT5G15500 36.404 445 252 11 5 423 6 445 1.12e-77 248
MS.gene80163.t1 AT5G54620 34.509 397 246 5 12 402 12 400 1.12e-73 237
MS.gene80163.t1 AT5G54610 36.772 378 228 6 1 370 1 375 3.48e-73 236
MS.gene80163.t1 AT1G14480 36.132 393 228 7 1 375 1 388 1.37e-72 234
MS.gene80163.t1 AT1G14480 32.570 393 199 8 1 375 1 345 4.40e-54 185
MS.gene80163.t1 AT5G15500 31.105 344 206 11 106 423 1 339 3.47e-34 130
MS.gene80163.t1 AT5G51160 31.636 275 162 5 53 311 31 295 6.75e-33 129
MS.gene80163.t1 AT3G09550 27.346 373 225 9 4 349 146 499 1.44e-27 115
MS.gene80163.t1 AT3G09550 27.346 373 225 9 4 349 146 499 1.44e-27 115
MS.gene80163.t1 AT3G09550 27.346 373 225 9 4 349 153 506 1.51e-27 115
MS.gene80163.t1 AT4G03500 26.509 381 209 14 39 372 194 550 1.37e-23 103
MS.gene80163.t1 AT4G03500 27.149 221 131 7 2 210 80 282 4.36e-11 65.5
MS.gene80163.t1 AT4G03500 26.509 381 209 14 39 372 194 550 1.37e-23 103
MS.gene80163.t1 AT4G03500 27.149 221 131 7 2 210 80 282 4.36e-11 65.5
MS.gene80163.t1 AT4G03500 26.509 381 209 14 39 372 217 573 1.84e-23 103
MS.gene80163.t1 AT4G03500 26.697 221 132 7 2 210 103 305 4.34e-11 65.5
MS.gene80163.t1 AT3G12360 25.708 459 282 16 8 427 136 574 1.93e-23 103
MS.gene80163.t1 AT3G12360 25.708 459 282 16 8 427 124 562 2.01e-23 103
MS.gene80163.t1 AT4G03460 29.927 274 159 11 83 346 245 495 8.03e-21 95.5
MS.gene80163.t1 AT4G03460 29.927 274 159 11 83 346 277 527 1.09e-20 95.1
MS.gene80163.t1 AT2G01680 22.174 460 261 12 3 425 96 495 2.51e-20 93.6
MS.gene80163.t1 AT5G02620 23.007 439 276 9 8 403 63 482 5.27e-20 92.8
MS.gene80163.t1 AT5G02620 23.007 439 276 9 8 403 63 482 5.27e-20 92.8
MS.gene80163.t1 AT5G02620 23.007 439 276 9 8 403 63 482 5.27e-20 92.8
MS.gene80163.t1 AT2G31820 22.276 413 261 11 4 377 192 583 6.43e-17 83.6
MS.gene80163.t1 AT5G02620 22.511 462 273 9 8 403 63 505 7.76e-17 83.2
MS.gene80163.t1 AT5G02620 22.511 462 273 9 8 403 63 505 7.76e-17 83.2
MS.gene80163.t1 AT1G34050 23.443 273 185 7 49 311 162 420 2.67e-15 78.6
MS.gene80163.t1 AT5G60070 23.585 318 194 6 3 309 110 389 3.06e-15 78.2
MS.gene80163.t1 AT1G03670 28.182 220 128 5 97 311 279 473 5.39e-15 77.4
MS.gene80163.t1 AT1G05640 22.556 399 204 12 38 339 114 504 5.92e-15 77.4
MS.gene80163.t1 AT1G10340 22.705 414 236 12 2 346 72 470 1.58e-14 75.9
MS.gene80163.t1 AT4G03480 26.354 277 180 7 41 311 357 615 1.33e-13 73.2
MS.gene80163.t1 AT4G03480 26.354 277 180 7 41 311 360 618 1.61e-13 73.2
MS.gene80163.t1 AT5G50140 25.333 225 134 7 5 227 31 223 1.37e-11 66.6
MS.gene80163.t1 AT4G03450 24.606 317 210 9 41 346 227 525 1.72e-11 66.6
MS.gene80163.t1 AT4G14390 22.102 371 248 9 42 395 276 622 2.89e-11 65.9
MS.gene80163.t1 AT4G03450 24.606 317 210 9 41 346 227 525 3.23e-11 65.9
MS.gene80163.t1 AT4G14390 22.102 371 248 9 42 395 295 641 3.44e-11 65.9
MS.gene80163.t1 AT4G11000 28.395 162 92 4 204 345 3 160 3.57e-11 63.5
MS.gene80163.t1 AT4G14390 22.115 416 268 12 8 395 255 642 7.88e-11 64.7

Find 70 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCATATTGCTGCAGCTTC+TGG 0.104530 3.4:-92291992 MS.gene80163:CDS
AAAATGCGAACGTACTTCTT+AGG 0.194723 3.4:-92290289 MS.gene80163:CDS
TGTTTGTTCATCTAAATTCT+TGG 0.276195 3.4:+92290606 None:intergenic
GAACGTACTTCTTAGGGTTT+TGG 0.277692 3.4:-92290282 MS.gene80163:CDS
GACCATACATTCGAACCTTT+TGG 0.281103 3.4:+92290029 None:intergenic
TGCATATTGCTGCAGCTTCT+GGG 0.291052 3.4:-92291991 MS.gene80163:CDS
GCTTGGAAACTAAACGAGTA+TGG 0.326774 3.4:-92291923 MS.gene80163:CDS
GACTAAAACCTTCATTTGCT+TGG 0.341585 3.4:-92291940 MS.gene80163:CDS
ATTTGTGCTGCTGCATTCAA+TGG 0.351754 3.4:+92292103 None:intergenic
TTGCACATTGCTACGCAAAC+TGG 0.361427 3.4:-92291788 MS.gene80163:CDS
TTCTTGAGATCTTGCTTGGT+TGG 0.367274 3.4:-92291652 MS.gene80163:CDS
GATGTTCTTGAGATCTTGCT+TGG 0.381604 3.4:-92291656 MS.gene80163:CDS
GACCCTCTCTCCCTTTGATT+CGG 0.391340 3.4:+92291819 None:intergenic
GCCCAAAAGGTTCGAATGTA+TGG 0.400483 3.4:-92290031 MS.gene80163:CDS
TCTTGTAGTTAAATTCTTGT+CGG 0.420938 3.4:-92291756 MS.gene80163:CDS
TTTGTTTCCTTCTGAAAACT+TGG 0.426086 3.4:+92289988 None:intergenic
ATTGAATGCAGCAGCACAAA+TGG 0.428078 3.4:-92292101 MS.gene80163:CDS
AAATGCGAACGTACTTCTTA+GGG 0.430694 3.4:-92290288 MS.gene80163:CDS
ATAGCACCAAGCTATACATT+TGG 0.447152 3.4:-92290127 MS.gene80163:CDS
TTCGAATGTATGGTCGTGAA+TGG 0.451564 3.4:-92290021 MS.gene80163:CDS
TGATTTCTGCACTATAAACT+TGG 0.455664 3.4:+92290554 None:intergenic
GCTATACAAACTGATTCAAA+TGG 0.456485 3.4:-92292065 MS.gene80163:CDS
AAAGACCTTGTCCGAATCAA+AGG 0.465762 3.4:-92291830 MS.gene80163:CDS
ATACATTACATTAGCCCAAA+AGG 0.466320 3.4:-92290044 MS.gene80163:CDS
TTATAGCGTTCTAAGATGTA+TGG 0.468594 3.4:+92292040 None:intergenic
TTTAGTTTCCAAGCAAATGA+AGG 0.469820 3.4:+92291932 None:intergenic
TCTTGCTTGGTTGGCTTCGA+AGG 0.470106 3.4:-92291643 MS.gene80163:CDS
AAATTCTTGTCGGTTTGTCC+AGG 0.494151 3.4:-92291746 MS.gene80163:CDS
TGTCATATTGTGCAGGCAGT+TGG 0.496735 3.4:-92290661 MS.gene80163:intron
CTCTCCCTTTGATTCGGACA+AGG 0.497968 3.4:+92291825 None:intergenic
GATCGTAGCTCTTCGTGAAG+TGG 0.498077 3.4:+92290478 None:intergenic
TGCAAAGGAGTATCAACAAA+TGG 0.503702 3.4:+92292010 None:intergenic
ATAACACTTATACGTCCTCT+TGG 0.503876 3.4:+92290205 None:intergenic
AGTTTATAGTGCAGAAATCA+AGG 0.506788 3.4:-92290551 MS.gene80163:CDS
TTTGTGCTGCTGCATTCAAT+GGG 0.507115 3.4:+92292104 None:intergenic
TTTAGATGAACAAACAGCAT+TGG 0.510720 3.4:-92290599 MS.gene80163:CDS
AGCTGGTGGAGGAAAATTAG+TGG 0.517201 3.4:-92290317 MS.gene80163:CDS
CTGAATTGGGAGGATGAAGC+AGG 0.520550 3.4:-92291581 MS.gene80163:CDS
TCTTAAACGAGTAACACAAA+TGG 0.523638 3.4:+92290429 None:intergenic
AAGGACATGTTGATTAAAGC+AGG 0.524636 3.4:-92290532 MS.gene80163:CDS
ATTAAAGCAGGAGCTTTACA+TGG 0.524711 3.4:-92290520 MS.gene80163:CDS
TACACTGCCACAAGTATACA+TGG 0.526508 3.4:+92290085 None:intergenic
TGTCCACTTGCTTTATTCTG+TGG 0.533431 3.4:-92290175 MS.gene80163:CDS
ACAAAACAAATATTACAGAA+TGG 0.533575 3.4:-92291876 MS.gene80163:CDS
ACAATTCCAAATGTATAGCT+TGG 0.534815 3.4:+92290121 None:intergenic
TTGAATGCAGCAGCACAAAT+GGG 0.536468 3.4:-92292100 MS.gene80163:CDS
TCGAATGTATGGTCGTGAAT+GGG 0.544937 3.4:-92290020 MS.gene80163:CDS
AAGACCTTGTCCGAATCAAA+GGG 0.554193 3.4:-92291829 MS.gene80163:CDS
GCTTGGTGCTATAACTAGCA+TGG 0.562144 3.4:+92290138 None:intergenic
TATTGAGAAGTGACATGTGC+AGG 0.574312 3.4:+92291549 None:intergenic
GGCTATTCCATGTATACTTG+TGG 0.575896 3.4:-92290092 MS.gene80163:CDS
CTTAACCCTCCAGCTGGTGG+AGG 0.584385 3.4:-92290328 MS.gene80163:CDS
CCTTGTGATGAGAACGTCTG+TGG 0.589242 3.4:+92291615 None:intergenic
CAACCACAGAATAAAGCAAG+TGG 0.589426 3.4:+92290172 None:intergenic
CCACAGACGTTCTCATCACA+AGG 0.592862 3.4:-92291615 MS.gene80163:CDS
AGCTCTTCGTGAAGTGGAGT+AGG 0.598129 3.4:+92290484 None:intergenic
ACAAACAGCATTGGACATAG+TGG 0.603971 3.4:-92290590 MS.gene80163:CDS
GTCCGAATCAAAGGGAGAGA+GGG 0.604625 3.4:-92291821 MS.gene80163:CDS
ACACTTAACCCTCCAGCTGG+TGG 0.610202 3.4:-92290331 MS.gene80163:CDS
GAAGCTGCAGCAATATGCAA+AGG 0.614052 3.4:+92291995 None:intergenic
TGTCCGAATCAAAGGGAGAG+AGG 0.614649 3.4:-92291822 MS.gene80163:CDS
ACTTCTCAATAGCTTCCCAC+AGG 0.615391 3.4:-92291537 MS.gene80163:intron
GTGACATCTTCAATACAACC+TGG 0.619746 3.4:+92291728 None:intergenic
GCAACACTTAACCCTCCAGC+TGG 0.624740 3.4:-92290334 MS.gene80163:CDS
GTTTGCGTAGCAATGTGCAA+AGG 0.652449 3.4:+92291791 None:intergenic
ATGTGTTTATTAATGCCAAG+AGG 0.652670 3.4:-92290220 MS.gene80163:CDS
GAGAAGTGACATGTGCAGGA+TGG 0.692918 3.4:+92291553 None:intergenic
AAAACGGGTTCTGAATTGGG+AGG 0.707905 3.4:-92291591 MS.gene80163:CDS
TACATTTGGAATTGTCACTG+TGG 0.721071 3.4:-92290113 MS.gene80163:CDS
GGTCTTTATTAATGTCTACG+AGG 0.768754 3.4:+92291846 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATATTAGTTTTAAATTG+AGG + chr3.4:92291152-92291171 None:intergenic 10.0%
!! CTTATTTATAAGAGATATTT+AGG + chr3.4:92291265-92291284 None:intergenic 15.0%
!! TATATTAATTAATTCAGCTA+TGG - chr3.4:92291334-92291353 MS.gene80163:intron 15.0%
!!! GTTTGTTATAATTTTTAAAG+TGG - chr3.4:92291081-92291100 MS.gene80163:intron 15.0%
!!! TATTTACTCATGTTTTTAAA+AGG - chr3.4:92290939-92290958 MS.gene80163:intron 15.0%
!!! TTTAAAACTAATATTTTCTC+TGG - chr3.4:92291155-92291174 MS.gene80163:intron 15.0%
!!! TTTTGATTTGACAATTAAAT+TGG - chr3.4:92291047-92291066 MS.gene80163:intron 15.0%
!! ACAAAACAAATATTACAGAA+TGG - chr3.4:92290218-92290237 MS.gene80163:CDS 20.0%
!! AGTAGAAAGAAAAGAAATAT+AGG + chr3.4:92290596-92290615 None:intergenic 20.0%
!! GTAGAAAGAAAAGAAATATA+GGG + chr3.4:92290595-92290614 None:intergenic 20.0%
!!! AATATTTGTTTTGTAAAGCA+AGG + chr3.4:92290212-92290231 None:intergenic 20.0%
!!! ATATGCCTTTAAAATACTTA+GGG - chr3.4:92290663-92290682 MS.gene80163:intron 20.0%
!!! TATATGCCTTTAAAATACTT+AGG - chr3.4:92290662-92290681 MS.gene80163:intron 20.0%
! AAACTAAATTAACTCAGCTA+TGG - chr3.4:92291407-92291426 MS.gene80163:intron 25.0%
! CACATAAATATCTACAAATG+TGG - chr3.4:92290620-92290639 MS.gene80163:CDS 25.0%
! TCTTGTAGTTAAATTCTTGT+CGG - chr3.4:92290338-92290357 MS.gene80163:CDS 25.0%
! TGTTTGTTCATCTAAATTCT+TGG + chr3.4:92291491-92291510 None:intergenic 25.0%
! TTGTTTGATCTTCAATGTTA+TGG + chr3.4:92290903-92290922 None:intergenic 25.0%
!!! AAGTACCCTAAGTATTTTAA+AGG + chr3.4:92290671-92290690 None:intergenic 25.0%
AACTAAGGGTCTCTTTATAA+GGG + chr3.4:92290861-92290880 None:intergenic 30.0%
ACAATTCCAAATGTATAGCT+TGG + chr3.4:92291976-92291995 None:intergenic 30.0%
AGTTTATAGTGCAGAAATCA+AGG - chr3.4:92291543-92291562 MS.gene80163:CDS 30.0%
ATACATTACATTAGCCCAAA+AGG - chr3.4:92292050-92292069 MS.gene80163:CDS 30.0%
ATGTGTTTATTAATGCCAAG+AGG - chr3.4:92291874-92291893 MS.gene80163:CDS 30.0%
TCTTAAACGAGTAACACAAA+TGG + chr3.4:92291668-92291687 None:intergenic 30.0%
TGATTTCTGCACTATAAACT+TGG + chr3.4:92291543-92291562 None:intergenic 30.0%
TTTAGATGAACAAACAGCAT+TGG - chr3.4:92291495-92291514 MS.gene80163:intron 30.0%
! AGTTTTCAGAAGGAAACAAA+TGG - chr3.4:92292109-92292128 MS.gene80163:CDS 30.0%
! GCTATACAAACTGATTCAAA+TGG - chr3.4:92290029-92290048 MS.gene80163:CDS 30.0%
! TTTAGTTTCCAAGCAAATGA+AGG + chr3.4:92290165-92290184 None:intergenic 30.0%
! TTTGTTTCCTTCTGAAAACT+TGG + chr3.4:92292109-92292128 None:intergenic 30.0%
! TTTTAGTCTCATAATCTCAG+TGG + chr3.4:92290142-92290161 None:intergenic 30.0%
! TTTTGTAAAGCAAGGTGTAT+GGG + chr3.4:92290204-92290223 None:intergenic 30.0%
!! TCAATCAAACTACTAGTCTT+TGG + chr3.4:92291289-92291308 None:intergenic 30.0%
!! TTATAGCGTTCTAAGATGTA+TGG + chr3.4:92290057-92290076 None:intergenic 30.0%
!!! GGTACTTTTTTGAAGAGTTT+TGG - chr3.4:92290684-92290703 MS.gene80163:intron 30.0%
!!! TTTTTTGAAGAGTTTTGGAG+AGG - chr3.4:92290689-92290708 MS.gene80163:intron 30.0%
AAAATGCGAACGTACTTCTT+AGG - chr3.4:92291805-92291824 MS.gene80163:CDS 35.0%
AAATGCGAACGTACTTCTTA+GGG - chr3.4:92291806-92291825 MS.gene80163:CDS 35.0%
ACTAAGGGTCTCTTTATAAG+GGG + chr3.4:92290860-92290879 None:intergenic 35.0%
ATAACACTTATACGTCCTCT+TGG + chr3.4:92291892-92291911 None:intergenic 35.0%
ATAGCACCAAGCTATACATT+TGG - chr3.4:92291967-92291986 MS.gene80163:CDS 35.0%
ATTAAAGCAGGAGCTTTACA+TGG - chr3.4:92291574-92291593 MS.gene80163:CDS 35.0%
CAACTAAGGGTCTCTTTATA+AGG + chr3.4:92290862-92290881 None:intergenic 35.0%
GACTAAAACCTTCATTTGCT+TGG - chr3.4:92290154-92290173 MS.gene80163:CDS 35.0%
GGTCTTTATTAATGTCTACG+AGG + chr3.4:92290251-92290270 None:intergenic 35.0%
GTTCTCATCACAAGGAAAAA+CGG - chr3.4:92290487-92290506 MS.gene80163:CDS 35.0%
TAAAGAGACCCTTAGTTGTT+TGG - chr3.4:92290864-92290883 MS.gene80163:intron 35.0%
TTCTCATCACAAGGAAAAAC+GGG - chr3.4:92290488-92290507 MS.gene80163:CDS 35.0%
! AAGGACATGTTGATTAAAGC+AGG - chr3.4:92291562-92291581 MS.gene80163:CDS 35.0%
! ACCATACATTCGAACCTTTT+GGG + chr3.4:92292067-92292086 None:intergenic 35.0%
! ACTTGTGGCAGTGTATTTTT+GGG - chr3.4:92292017-92292036 MS.gene80163:CDS 35.0%
! GATGTATCCAAGTTTTCAGA+AGG - chr3.4:92292099-92292118 MS.gene80163:CDS 35.0%
! GTTTTGTAAAGCAAGGTGTA+TGG + chr3.4:92290205-92290224 None:intergenic 35.0%
! TACATTTGGAATTGTCACTG+TGG - chr3.4:92291981-92292000 MS.gene80163:CDS 35.0%
! TACTTGTGGCAGTGTATTTT+TGG - chr3.4:92292016-92292035 MS.gene80163:CDS 35.0%
!! TGCAAAGGAGTATCAACAAA+TGG + chr3.4:92290087-92290106 None:intergenic 35.0%
AAAGACCTTGTCCGAATCAA+AGG - chr3.4:92290264-92290283 MS.gene80163:CDS 40.0%
AAGACCTTGTCCGAATCAAA+GGG - chr3.4:92290265-92290284 MS.gene80163:CDS 40.0%
ATCTCGAGACCAAACAACTA+AGG + chr3.4:92290876-92290895 None:intergenic 40.0%
ATGGTCATGTCATATTGTGC+AGG - chr3.4:92291426-92291445 MS.gene80163:intron 40.0%
ATTGAATGCAGCAGCACAAA+TGG - chr3.4:92289993-92290012 MS.gene80163:CDS 40.0%
CAACCACAGAATAAAGCAAG+TGG + chr3.4:92291925-92291944 None:intergenic 40.0%
CTAAGGGTCTCTTTATAAGG+GGG + chr3.4:92290859-92290878 None:intergenic 40.0%
GATGTTCTTGAGATCTTGCT+TGG - chr3.4:92290438-92290457 MS.gene80163:CDS 40.0%
GCTTGGAAACTAAACGAGTA+TGG - chr3.4:92290171-92290190 MS.gene80163:CDS 40.0%
GGCTATTCCATGTATACTTG+TGG - chr3.4:92292002-92292021 MS.gene80163:CDS 40.0%
GTGACATCTTCAATACAACC+TGG + chr3.4:92290369-92290388 None:intergenic 40.0%
TAAGGGTCTCTTTATAAGGG+GGG + chr3.4:92290858-92290877 None:intergenic 40.0%
TACACTGCCACAAGTATACA+TGG + chr3.4:92292012-92292031 None:intergenic 40.0%
TATTGAGAAGTGACATGTGC+AGG + chr3.4:92290548-92290567 None:intergenic 40.0%
TCGAATGTATGGTCGTGAAT+GGG - chr3.4:92292074-92292093 MS.gene80163:CDS 40.0%
TCTCGAGACCAAACAACTAA+GGG + chr3.4:92290875-92290894 None:intergenic 40.0%
TTCGAATGTATGGTCGTGAA+TGG - chr3.4:92292073-92292092 MS.gene80163:CDS 40.0%
TTCTTGAGATCTTGCTTGGT+TGG - chr3.4:92290442-92290461 MS.gene80163:CDS 40.0%
TTGAATGCAGCAGCACAAAT+GGG - chr3.4:92289994-92290013 MS.gene80163:CDS 40.0%
! ACAAACAGCATTGGACATAG+TGG - chr3.4:92291504-92291523 MS.gene80163:intron 40.0%
! ATTTGTGCTGCTGCATTCAA+TGG + chr3.4:92289994-92290013 None:intergenic 40.0%
! GAACGTACTTCTTAGGGTTT+TGG - chr3.4:92291812-92291831 MS.gene80163:CDS 40.0%
! GACCATACATTCGAACCTTT+TGG + chr3.4:92292068-92292087 None:intergenic 40.0%
! TGTCCACTTGCTTTATTCTG+TGG - chr3.4:92291919-92291938 MS.gene80163:CDS 40.0%
! TTTGTGCTGCTGCATTCAAT+GGG + chr3.4:92289993-92290012 None:intergenic 40.0%
!! AAATTCTTGTCGGTTTGTCC+AGG - chr3.4:92290348-92290367 MS.gene80163:CDS 40.0%
!! AGGAAAAACGGGTTCTGAAT+TGG - chr3.4:92290499-92290518 MS.gene80163:CDS 40.0%
!! CTTGTGGCAGTGTATTTTTG+GGG - chr3.4:92292018-92292037 MS.gene80163:CDS 40.0%
!! GGAAAAACGGGTTCTGAATT+GGG - chr3.4:92290500-92290519 MS.gene80163:CDS 40.0%
!!! TTTGAAGAGTTTTGGAGAGG+AGG - chr3.4:92290692-92290711 MS.gene80163:intron 40.0%
ACTTCTCAATAGCTTCCCAC+AGG - chr3.4:92290557-92290576 MS.gene80163:CDS 45.0%
AGCTGGTGGAGGAAAATTAG+TGG - chr3.4:92291777-92291796 MS.gene80163:CDS 45.0%
CTTTATAAGGGGGGTCTGAA+GGG + chr3.4:92290849-92290868 None:intergenic 45.0%
GAAGCTGCAGCAATATGCAA+AGG + chr3.4:92290102-92290121 None:intergenic 45.0%
GCCCAAAAGGTTCGAATGTA+TGG - chr3.4:92292063-92292082 MS.gene80163:CDS 45.0%
GTTTGCGTAGCAATGTGCAA+AGG + chr3.4:92290306-92290325 None:intergenic 45.0%
TCTTTATAAGGGGGGTCTGA+AGG + chr3.4:92290850-92290869 None:intergenic 45.0%
TGCATATTGCTGCAGCTTCT+GGG - chr3.4:92290103-92290122 MS.gene80163:CDS 45.0%
TGTCATATTGTGCAGGCAGT+TGG - chr3.4:92291433-92291452 MS.gene80163:intron 45.0%
TTGCACATTGCTACGCAAAC+TGG - chr3.4:92290306-92290325 MS.gene80163:CDS 45.0%
TTGCATATTGCTGCAGCTTC+TGG - chr3.4:92290102-92290121 MS.gene80163:CDS 45.0%
TTTATAAGGGGGGTCTGAAG+GGG + chr3.4:92290848-92290867 None:intergenic 45.0%
! ACTAATTTTCCTCCACCAGC+TGG + chr3.4:92291778-92291797 None:intergenic 45.0%
!! AAAACGGGTTCTGAATTGGG+AGG - chr3.4:92290503-92290522 MS.gene80163:CDS 45.0%
!! GCTTGGTGCTATAACTAGCA+TGG + chr3.4:92291959-92291978 None:intergenic 45.0%
AGCTCTTCGTGAAGTGGAGT+AGG + chr3.4:92291613-92291632 None:intergenic 50.0%
AGGGATGTGTCTCATACCTG+TGG + chr3.4:92290576-92290595 None:intergenic 50.0%
CCACAGACGTTCTCATCACA+AGG - chr3.4:92290479-92290498 MS.gene80163:CDS 50.0%
CCTTGTGATGAGAACGTCTG+TGG + chr3.4:92290482-92290501 None:intergenic 50.0%
CTGAATTGGGAGGATGAAGC+AGG - chr3.4:92290513-92290532 MS.gene80163:CDS 50.0%
GACCCTCTCTCCCTTTGATT+CGG + chr3.4:92290278-92290297 None:intergenic 50.0%
GAGAAGTGACATGTGCAGGA+TGG + chr3.4:92290544-92290563 None:intergenic 50.0%
GATCGTAGCTCTTCGTGAAG+TGG + chr3.4:92291619-92291638 None:intergenic 50.0%
GGGATGTGTCTCATACCTGT+GGG + chr3.4:92290575-92290594 None:intergenic 50.0%
GTCCGAATCAAAGGGAGAGA+GGG - chr3.4:92290273-92290292 MS.gene80163:CDS 50.0%
TGTCCGAATCAAAGGGAGAG+AGG - chr3.4:92290272-92290291 MS.gene80163:CDS 50.0%
! AATTTTCCTCCACCAGCTGG+AGG + chr3.4:92291775-92291794 None:intergenic 50.0%
! ATTTTCCTCCACCAGCTGGA+GGG + chr3.4:92291774-92291793 None:intergenic 50.0%
!! CTCTCCCTTTGATTCGGACA+AGG + chr3.4:92290272-92290291 None:intergenic 50.0%
!! TCTTGCTTGGTTGGCTTCGA+AGG - chr3.4:92290451-92290470 MS.gene80163:CDS 50.0%
ACACTTAACCCTCCAGCTGG+TGG - chr3.4:92291763-92291782 MS.gene80163:CDS 55.0%
GCAACACTTAACCCTCCAGC+TGG - chr3.4:92291760-92291779 MS.gene80163:CDS 55.0%
CTTAACCCTCCAGCTGGTGG+AGG - chr3.4:92291766-92291785 MS.gene80163:CDS 60.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 92289982 92292134 92289982 ID=MS.gene80163
chr3.4 mRNA 92289982 92292134 92289982 ID=MS.gene80163.t1;Parent=MS.gene80163
chr3.4 exon 92291538 92292134 92291538 ID=MS.gene80163.t1.exon1;Parent=MS.gene80163.t1
chr3.4 CDS 92291538 92292134 92291538 ID=cds.MS.gene80163.t1;Parent=MS.gene80163.t1
chr3.4 exon 92289982 92290668 92289982 ID=MS.gene80163.t1.exon2;Parent=MS.gene80163.t1
chr3.4 CDS 92289982 92290668 92289982 ID=cds.MS.gene80163.t1;Parent=MS.gene80163.t1
Gene Sequence

>MS.gene80163

ATGAATGACCCATTGAATGCAGCAGCACAAATGGGAGATATAGACTTGCTATACAAACTGATTCAAATGGATCCATACATCTTAGAACGCTATAATTTAATACCATTTGTTGATACTCCTTTGCATATTGCTGCAGCTTCTGGGCATGTAAGTTTTGCCACTGAGATTATGAGACTAAAACCTTCATTTGCTTGGAAACTAAACGAGTATGGACTCAGTCCCATACACCTTGCTTTACAAAACAAATATTACAGAATGGTGTGTCGCCTCGTAGACATTAATAAAGACCTTGTCCGAATCAAAGGGAGAGAGGGTCTAACTCCTTTGCACATTGCTACGCAAACTGGAAACTTTGATCTTGTAGTTAAATTCTTGTCGGTTTGTCCAGGTTGTATTGAAGATGTCACAGTTCGAAGTGAGACTGCTTTGCATCTTGCTGTGAAATATAAGCAGTTTGATGTTCTTGAGATCTTGCTTGGTTGGCTTCGAAGGACATGCCACAGACGTTCTCATCACAAGGAAAAACGGGTTCTGAATTGGGAGGATGAAGCAGGCAACACCATCCTGCACATGTCACTTCTCAATAGCTTCCCACAGGTATGAGACACATCCCTATATTTCTTTTCTTTCTACTTTTTCACATAAATATCTACAAATGTGGCTGATGCGACTGCAATGCATATATGCCTTTAAAATACTTAGGGTACTTTTTTGAAGAGTTTTGGAGAGGAGGAGAAGAGACTTGTTTTTCTTTTGTATTACGAACACTTTAAAATATTTTTTAAAATCAATCAAAAGTTGAAGTATCGTATGATCATTTATCATTTTTTTGAATAACAAATTTATATTAAAATATAGACTTACCCCTTCAGACCCCCCTTATAAAGAGACCCTTAGTTGTTTGGTCTCGAGATATTTCCATAACATTGAAGATCAAACAAAGTTTTATGTAATCGATATTTACTCATGTTTTTAAAAGGATAATATACATTTATTTGATACTATTTAATAAATATCAAATTTTGAAATTTAATAAAATTTTGTTAAATTAATCAACTCAATGACTTTTGATTTGACAATTAAATTGGTGAAATTCAGTGTTTGTTATAATTTTTAAAGTGGAGTAACTCAGTTGAGAGTTAATCTTAGAGCGATTGCAGTTACGTACCTCAATTTAAAACTAATATTTTCTCTGGTCTTATTTATAAGAGACATTTGAGTCAATAAAAGTTAGATGTATCTGATTAAGATTTTGAATTAGATAGATCAACTTTTGTTGACCTAAATATCTCTTATAAATAAGACCAAAGACTAGTAGTTTGATTGAAAATCAAAATCAATCTAAGTTTCAATATATTAATTAATTCAGCTATGGTGTAGTTTGACATTTTCAAATTATTAATAATTTTTCTCTTCTATTTAAAAAAACTAAATTAACTCAGCTATGGTCATGTCATATTGTGCAGGCAGTTGGATTGTTGATAGATAGCAACATAGACATAAATGCCAAGAATTTAGATGAACAAACAGCATTGGACATAGTGGAGCAGATTCAGAGCCAAGTTTATAGTGCAGAAATCAAGGACATGTTGATTAAAGCAGGAGCTTTACATGGTTTTTCACTTGCTCCTACTCCACTTCACGAAGAGCTACGATCAAAAATCACATTTAACGAGAGAATAGCCATTTGTGTTACTCGTTTAAGAAGAAGAATTTCAAATGACACAAGAAACGCTTTGTTAGTAGTTGCTATTCTTTTTGCAACAAGTGCATATGAAGCAACACTTAACCCTCCAGCTGGTGGAGGAAAATTAGTGGTGATGAAAATGCGAACGTACTTCTTAGGGTTTTGGTCGATTAATACATTTTCATTTTATGTTTCAGTTTTGATGATGTGTTTATTAATGCCAAGAGGACGTATAAGTGTTATAGTAACTTGTCCACTTGCTTTATTCTGTGGTTGTTATATGTTTTCCATGCTAGTTATAGCACCAAGCTATACATTTGGAATTGTCACTGTGGCTATTCCATGTATACTTGTGGCAGTGTATTTTTGGGGAGCTATGATATACATTACATTAGCCCAAAAGGTTCGAATGTATGGTCGTGAATGGGAAGATGTATCCAAGTTTTCAGAAGGAAACAAATGGTAA
Protein sequence

>MS.gene80163.t1

MNDPLNAAAQMGDIDLLYKLIQMDPYILERYNLIPFVDTPLHIAAASGHVSFATEIMRLKPSFAWKLNEYGLSPIHLALQNKYYRMVCRLVDINKDLVRIKGREGLTPLHIATQTGNFDLVVKFLSVCPGCIEDVTVRSETALHLAVKYKQFDVLEILLGWLRRTCHRRSHHKEKRVLNWEDEAGNTILHMSLLNSFPQAVGLLIDSNIDINAKNLDEQTALDIVEQIQSQVYSAEIKDMLIKAGALHGFSLAPTPLHEELRSKITFNERIAICVTRLRRRISNDTRNALLVVAILFATSAYEATLNPPAGGGKLVVMKMRTYFLGFWSINTFSFYVSVLMMCLLMPRGRISVIVTCPLALFCGCYMFSMLVIAPSYTFGIVTVAIPCILVAVYFWGAMIYITLAQKVRMYGREWEDVSKFSEGNKW