AlfalfaGEDB | MS.gene71204

M. sativa cultivar XinJiangDaYe / MS.gene71204

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF12796.10 PF13857.9 PF13962.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene71204.t1	AET03258.2	87.9	437	53	0	1	437	1067	1503	8.90E-175	623.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene71204.t1	Q8GYH5	39.5	304	173	4	8	303	4	304	1.2e-18	95.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene71204.t1	G7LH09	87.9	437	53	0	1	437	1067	1503	6.4e-175	623.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene71204	MS.gene21734	PPI
MS.gene068249	MS.gene71204	PPI
MS.gene71204	MS.gene30177	PPI
MS.gene71204	MS.gene70008	PPI
MS.gene71204	MS.gene008767	PPI
MS.gene71204	MS.gene23811	PPI
MS.gene71204	MS.gene071113	PPI
MS.gene71204	MS.gene90400	PPI
MS.gene71204	MS.gene25244	PPI
MS.gene71204	MS.gene82740	PPI
MS.gene71204	MS.gene48655	PPI
MS.gene71204	MS.gene09785	PPI
MS.gene71204	MS.gene64441	PPI
MS.gene71204	MS.gene066046	PPI
MS.gene71204	MS.gene007486	PPI
MS.gene71204	MS.gene23810	PPI
MS.gene71204	MS.gene51335	PPI
MS.gene039682	MS.gene71204	PPI
MS.gene71204	MS.gene027073	PPI
MS.gene71204	MS.gene027072	PPI
MS.gene71204	MS.gene65463	PPI
MS.gene71204	MS.gene007485	PPI
MS.gene47479	MS.gene71204	PPI
MS.gene71204	MS.gene23196	PPI
MS.gene71204	MS.gene067705	PPI
MS.gene71204	MS.gene025229	PPI
MS.gene71204	MS.gene30433	PPI
MS.gene71204	MS.gene043742	PPI
MS.gene71204	MS.gene027075	PPI
MS.gene71204	MS.gene027441	PPI
MS.gene71204	MS.gene061932	PPI
MS.gene36842	MS.gene71204	PPI
MS.gene71204	MS.gene050457	PPI
MS.gene71204	MS.gene99973	PPI
MS.gene71204	MS.gene30174	PPI
MS.gene71204	MS.gene008833	PPI
MS.gene066501	MS.gene71204	PPI
MS.gene71204	MS.gene064542	PPI
MS.gene056455	MS.gene71204	PPI
MS.gene71204	MS.gene91775	PPI
MS.gene71204	MS.gene051003	PPI
MS.gene71204	MS.gene25515	PPI
MS.gene71204	MS.gene89986	PPI
MS.gene71204	MS.gene050270	PPI
MS.gene71204	MS.gene08470	PPI
MS.gene69965	MS.gene71204	PPI
MS.gene71204	MS.gene34712	PPI
MS.gene71204	MS.gene058128	PPI
MS.gene26532	MS.gene71204	PPI
MS.gene71204	MS.gene39623	PPI
MS.gene71204	MS.gene007487	PPI
MS.gene71204	MS.gene065848	PPI
MS.gene71204	MS.gene037184	PPI
MS.gene71204	MS.gene004844	PPI
MS.gene71204	MS.gene068249	PPI
MS.gene71204	MS.gene99588	PPI
MS.gene30172	MS.gene71204	PPI
MS.gene71204	MS.gene038519	PPI
MS.gene71204	MS.gene41291	PPI
MS.gene71204	MS.gene27223	PPI
MS.gene71204	MS.gene042783	PPI
MS.gene23852	MS.gene71204	PPI
MS.gene97943	MS.gene71204	PPI
MS.gene67157	MS.gene71204	PPI
MS.gene71204	MS.gene97943	PPI
MS.gene71204	MS.gene050272	PPI
MS.gene71204	MS.gene63085	PPI
MS.gene71204	MS.gene26021	PPI
MS.gene71204	MS.gene050456	PPI
MS.gene010910	MS.gene71204	PPI
MS.gene71204	MS.gene51258	PPI
MS.gene71204	MS.gene32723	PPI
MS.gene71204	MS.gene053363	PPI
MS.gene063222	MS.gene71204	PPI
MS.gene71204	MS.gene066467	PPI
MS.gene71204	MS.gene34651	PPI
MS.gene69687	MS.gene71204	PPI
MS.gene71204	MS.gene32726	PPI
MS.gene035805	MS.gene71204	PPI
MS.gene71204	MS.gene71122	PPI
MS.gene71204	MS.gene22482	PPI
MS.gene71204	MS.gene24922	PPI
MS.gene71204	MS.gene54764	PPI
MS.gene71204	MS.gene052956	PPI
MS.gene71204	MS.gene60920	PPI
MS.gene71204	MS.gene73119	PPI
MS.gene71204	MS.gene92243	PPI
MS.gene23810	MS.gene71204	PPI
MS.gene71204	MS.gene037950	PPI
MS.gene71204	MS.gene34009	PPI
MS.gene71204	MS.gene51262	PPI
MS.gene71204	MS.gene26672	PPI
MS.gene71204	MS.gene018518	PPI
MS.gene71204	MS.gene043662	PPI
MS.gene78281	MS.gene71204	PPI
MS.gene71204	MS.gene014931	PPI
MS.gene71204	MS.gene21888	PPI
MS.gene71204	MS.gene066500	PPI
MS.gene24806	MS.gene71204	PPI
MS.gene071113	MS.gene71204	PPI
MS.gene71204	MS.gene066464	PPI
MS.gene71204	MS.gene07706	PPI
MS.gene71204	MS.gene25024	PPI
MS.gene71204	MS.gene012203	PPI
MS.gene027071	MS.gene71204	PPI
MS.gene71204	MS.gene26019	PPI
MS.gene71204	MS.gene022109	PPI
MS.gene71204	MS.gene30176	PPI
MS.gene71204	MS.gene041770	PPI
MS.gene71204	MS.gene81055	PPI
MS.gene71204	MS.gene65775	PPI
MS.gene066464	MS.gene71204	PPI
MS.gene71204	MS.gene99554	PPI
MS.gene71204	MS.gene80163	PPI
MS.gene71204	MS.gene02469	PPI
MS.gene71204	MS.gene33377	PPI
MS.gene07706	MS.gene71204	PPI
MS.gene027074	MS.gene71204	PPI
MS.gene71204	MS.gene024753	PPI
MS.gene71204	MS.gene045937	PPI
MS.gene71204	MS.gene51278	PPI
MS.gene71204	MS.gene69966	PPI
MS.gene010912	MS.gene71204	PPI
MS.gene71204	MS.gene87218	PPI
MS.gene23811	MS.gene71204	PPI
MS.gene71204	MS.gene85534	PPI
MS.gene51335	MS.gene71204	PPI
MS.gene71204	MS.gene23881	PPI
MS.gene005995	MS.gene71204	PPI
MS.gene71204	MS.gene066501	PPI
MS.gene037950	MS.gene71204	PPI
MS.gene71204	MS.gene062662	PPI
MS.gene71204	MS.gene97379	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene71204.t1	MTR_8g066670	89.016	437	48	0	1	437	1067	1503	0.0	795
MS.gene71204.t1	MTR_8g072510	58.124	437	178	4	1	433	1	436	1.68e-172	491
MS.gene71204.t1	MTR_8g062410	58.685	426	168	3	1	424	1	420	2.89e-158	455
MS.gene71204.t1	MTR_3g016210	57.210	423	162	6	1	404	1	423	4.01e-154	444
MS.gene71204.t1	MTR_7g021090	58.543	398	151	5	1	392	1	390	5.40e-153	440
MS.gene71204.t1	MTR_8g039620	55.742	418	178	4	2	413	6	422	2.50e-152	439
MS.gene71204.t1	MTR_7g451420	67.412	313	100	2	1	312	1	312	2.89e-149	427
MS.gene71204.t1	MTR_8g467780	55.269	427	174	8	1	412	1	425	1.14e-147	428
MS.gene71204.t1	MTR_8g069880	55.455	440	189	6	4	438	2	439	3.45e-146	425
MS.gene71204.t1	MTR_8g073260	53.132	431	183	7	7	427	9	430	6.59e-141	410
MS.gene71204.t1	MTR_8g040930	55.085	354	156	2	52	402	2	355	1.02e-130	382
MS.gene71204.t1	MTR_8g041240	55.085	354	156	2	52	402	2	355	1.02e-130	382
MS.gene71204.t1	MTR_8g068990	53.125	384	163	4	5	385	8	377	3.06e-129	381
MS.gene71204.t1	MTR_1g059930	59.322	354	141	3	6	357	9	361	2.29e-127	375
MS.gene71204.t1	MTR_8g073275	41.892	444	175	10	52	427	2	430	2.51e-97	299
MS.gene71204.t1	MTR_3g105650	37.986	437	230	6	9	436	5	409	4.16e-93	288
MS.gene71204.t1	MTR_1g069110	24.074	432	278	12	12	422	21	423	9.06e-28	115
MS.gene71204.t1	MTR_1g115575	24.672	381	226	9	12	367	138	482	4.54e-18	87.0
MS.gene71204.t1	MTR_4g062100	23.765	324	210	4	9	308	74	384	6.10e-18	86.7
MS.gene71204.t1	MTR_7g117620	25.740	338	203	7	44	359	119	430	8.44e-18	86.3
MS.gene71204.t1	MTR_7g099580	24.834	302	166	8	21	312	24	274	2.59e-17	83.2
MS.gene71204.t1	MTR_2g101320	27.650	217	140	3	40	249	137	343	2.98e-17	84.7
MS.gene71204.t1	MTR_2g101310	26.939	245	148	6	40	265	137	369	1.43e-16	82.4
MS.gene71204.t1	MTR_7g100390	23.467	375	238	9	9	359	53	402	1.90e-16	82.0
MS.gene71204.t1	MTR_2g101310	26.939	245	148	6	40	265	137	369	3.62e-16	80.9
MS.gene71204.t1	MTR_7g099570	26.667	225	149	4	12	229	12	227	3.93e-16	81.3
MS.gene71204.t1	MTR_2g096190	23.765	324	210	6	9	308	70	380	1.25e-15	79.3
MS.gene71204.t1	MTR_5g085830	25.778	225	151	7	43	262	277	490	2.83e-12	69.3
MS.gene71204.t1	MTR_8g105260	23.077	169	128	1	2	170	95	261	2.53e-11	65.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene71204.t1	AT4G10720	38.303	436	235	8	1	423	1	415	1.87e-90	281
MS.gene71204.t1	AT4G10720	37.656	401	216	7	1	391	1	377	3.46e-85	267
MS.gene71204.t1	AT5G15500	39.143	350	192	8	9	349	6	343	6.45e-73	236
MS.gene71204.t1	AT1G14480	33.563	435	241	8	8	426	4	406	7.62e-73	236
MS.gene71204.t1	AT1G14500	32.924	407	238	6	1	396	1	383	1.77e-70	229
MS.gene71204.t1	AT5G54620	38.889	306	180	2	8	306	4	309	6.40e-68	223
MS.gene71204.t1	AT5G54610	37.829	304	178	4	8	303	4	304	7.61e-63	209
MS.gene71204.t1	AT1G14480	32.619	420	222	10	8	426	4	363	1.92e-57	194
MS.gene71204.t1	AT5G15500	34.274	248	142	8	111	349	2	237	5.79e-33	127
MS.gene71204.t1	AT5G51160	27.202	386	225	12	63	412	35	400	1.81e-30	122
MS.gene71204.t1	AT4G03500	24.270	445	210	14	9	367	117	520	7.97e-17	83.2
MS.gene71204.t1	AT4G03500	24.270	445	210	14	9	367	117	520	7.97e-17	83.2
MS.gene71204.t1	AT4G03500	24.045	445	211	14	9	367	140	543	8.14e-17	83.2
MS.gene71204.t1	AT3G12360	23.560	382	229	9	12	367	124	468	1.05e-16	82.8
MS.gene71204.t1	AT3G12360	23.560	382	229	9	12	367	136	480	1.16e-16	82.8
MS.gene71204.t1	AT1G05640	26.822	343	194	12	44	359	191	503	5.29e-16	80.9
MS.gene71204.t1	AT3G09550	23.499	383	228	9	12	367	150	494	6.92e-16	80.5
MS.gene71204.t1	AT3G09550	23.499	383	228	9	12	367	150	494	6.92e-16	80.5
MS.gene71204.t1	AT3G09550	23.499	383	228	9	12	367	157	501	7.44e-16	80.5
MS.gene71204.t1	AT5G02620	23.636	385	243	10	2	359	53	413	3.91e-15	77.8
MS.gene71204.t1	AT5G02620	23.636	385	243	10	2	359	53	413	3.91e-15	77.8
MS.gene71204.t1	AT5G02620	23.636	385	243	10	2	359	53	413	3.91e-15	77.8
MS.gene71204.t1	AT2G31820	23.964	338	209	8	44	359	227	538	4.32e-15	78.2
MS.gene71204.t1	AT1G07710	25.373	335	209	8	2	311	63	381	1.30e-14	76.3
MS.gene71204.t1	AT1G07710	25.373	335	209	8	2	311	63	381	1.30e-14	76.3
MS.gene71204.t1	AT5G54710	24.337	415	227	14	9	367	151	534	3.05e-14	75.5
MS.gene71204.t1	AT4G03450	25.000	360	188	12	19	367	237	525	3.60e-14	75.1
MS.gene71204.t1	AT5G54710	24.337	415	227	14	9	367	106	489	3.63e-14	75.1
MS.gene71204.t1	AT4G03450	25.000	360	188	12	19	367	237	525	4.22e-14	74.7
MS.gene71204.t1	AT4G03460	26.335	281	147	7	102	367	260	495	7.24e-14	73.9
MS.gene71204.t1	AT4G03460	26.335	281	147	7	102	367	292	527	7.95e-14	73.9
MS.gene71204.t1	AT5G60070	26.205	332	200	13	9	311	77	392	1.28e-13	73.2
MS.gene71204.t1	AT1G03670	26.429	280	153	6	99	367	277	514	2.55e-13	72.4
MS.gene71204.t1	AT1G34050	21.411	397	254	10	65	433	174	540	1.27e-12	70.1
MS.gene71204.t1	AT4G03480	24.214	318	198	10	60	367	375	659	2.74e-11	66.2
MS.gene71204.t1	AT4G03480	24.214	318	198	10	60	367	372	656	2.83e-11	65.9
MS.gene71204.t1	AT5G02620	22.549	408	242	11	2	359	53	436	3.86e-11	65.5
MS.gene71204.t1	AT5G02620	22.549	408	242	11	2	359	53	436	3.86e-11	65.5

Find 85 sgRNAs with CRISPR-Local

Find 114 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTTACACATCGCCGCATTTA+AGG	0.149352	8.3:-36372414	MS.gene71204:CDS
ATTGATTTCCCAGCATTTCT+AGG	0.271134	8.3:+36370984	None:intergenic
TCTATATTCTCTAAAATAGA+TGG	0.276484	8.3:+36372461	None:intergenic
TCCCGTTGGTGGAGTTTATC+AGG	0.296151	8.3:-36371063	MS.gene71204:CDS
AACTTTCAGTTTATGAAGAT+TGG	0.324299	8.3:+36372535	None:intergenic
TTAACACTTTATATATAATC+TGG	0.327167	8.3:+36371189	None:intergenic
GGTAGAAATTGTAGAATTAG+AGG	0.347138	8.3:+36371007	None:intergenic
TAGAATCAGGACAAGCAATT+AGG	0.348394	8.3:+36372162	None:intergenic
CAATTAGGAACTTAGCTAAA+AGG	0.389011	8.3:+36372177	None:intergenic
TTACACATCGCCGCATTTAA+GGG	0.411662	8.3:-36372413	MS.gene71204:CDS
TAAATGCGGCGATGTGTAAA+GGG	0.412669	8.3:+36372417	None:intergenic
GTAACTATTCTGATACCAAC+TGG	0.413101	8.3:-36370891	MS.gene71204:CDS
ATCATAGAACTGGCATGTGC+AGG	0.427241	8.3:+36370792	None:intergenic
TTCAGTTTATGAAGATTGGT+TGG	0.427494	8.3:+36372539	None:intergenic
TGTTGAATAGCTACGTGAGT+TGG	0.445717	8.3:+36372314	None:intergenic
ATTTCTACCCCTAGAAATGC+TGG	0.446001	8.3:-36370993	MS.gene71204:CDS
TATGTTGAAAATCATAGAAC+TGG	0.450365	8.3:+36370782	None:intergenic
GGGAGTCAATGCAGATTGAT+AGG	0.457308	8.3:+36371082	None:intergenic
TCATAATTTCAAGGGCAAAC+CGG	0.459972	8.3:+36372384	None:intergenic
TAGAATATAGAACACTAAAT+CGG	0.460464	8.3:-36372016	MS.gene71204:CDS
ACCTGATAAACTCCACCAAC+GGG	0.464482	8.3:+36371062	None:intergenic
TTGATTTCCCAGCATTTCTA+GGG	0.467353	8.3:+36370985	None:intergenic
TCAGTTTATGAAGATTGGTT+GGG	0.468399	8.3:+36372540	None:intergenic
TTAAATGCGGCGATGTGTAA+AGG	0.468604	8.3:+36372416	None:intergenic
GTTGAATAGCTACGTGAGTT+GGG	0.475458	8.3:+36372315	None:intergenic
TAGAGGAGCAACGTAACTCT+TGG	0.476990	8.3:-36371136	MS.gene71204:CDS
CCGCATTTAAGGGGCATCTC+CGG	0.479615	8.3:-36372403	MS.gene71204:CDS
GTCACGTCTTCAATAGAATC+AGG	0.480175	8.3:+36372149	None:intergenic
AAGAATATATTGAGGAATGC+TGG	0.480580	8.3:-36371272	MS.gene71204:CDS
CTGAAAGTTGCAGCTGAAGA+AGG	0.483015	8.3:-36372521	MS.gene71204:CDS
CATTAGAAATGATATATTAG+AGG	0.484616	8.3:-36371153	MS.gene71204:CDS
TAGTCTAGTGGCAGCTCCAG+TGG	0.492179	8.3:-36370859	MS.gene71204:CDS
CCCTTCCTTTGACTCTGACA+AGG	0.496694	8.3:+36372246	None:intergenic
TCTGCATTGACTCCCGTTGG+TGG	0.498713	8.3:-36371074	MS.gene71204:CDS
GTTTCAAATTCATAATTTCA+AGG	0.506445	8.3:+36372375	None:intergenic
AATAACCTTGTCAGAGTCAA+AGG	0.508164	8.3:-36372251	MS.gene71204:CDS
TTTCAAATTCATAATTTCAA+GGG	0.509472	8.3:+36372376	None:intergenic
GCTGGGAAATCAATATTGTC+AGG	0.509963	8.3:-36370975	MS.gene71204:CDS
TTTGGAGAACAAAACAGCAT+TGG	0.514840	8.3:-36371318	MS.gene71204:CDS
TGATAGTCTTTGTTCAAGTG+TGG	0.516858	8.3:+36371217	None:intergenic
CCTCCTCTATGAAGTAATTG+AGG	0.518436	8.3:-36372489	MS.gene71204:CDS
GCATCGATGACTTGTGAGCT+AGG	0.519112	8.3:+36371242	None:intergenic
TAACACTTTATATATAATCT+GGG	0.529640	8.3:+36371190	None:intergenic
TCCTTCATGAACGCATTGCT+CGG	0.529877	8.3:-36370701	MS.gene71204:CDS
TCATAGAACTGGCATGTGCA+GGG	0.530470	8.3:+36370793	None:intergenic
TCAACAAAACCATAAAAGAA+TGG	0.534119	8.3:-36372297	MS.gene71204:CDS
TCCGAGCAATGCGTTCATGA+AGG	0.539709	8.3:+36370700	None:intergenic
AGTCTAGTGGCAGCTCCAGT+GGG	0.540215	8.3:-36370858	MS.gene71204:CDS
CTATATTCTCTAAAATAGAT+GGG	0.541917	8.3:+36372462	None:intergenic
CGGATTAAGCTTCAAAGCAA+AGG	0.559066	8.3:+36372349	None:intergenic
AACACTAAATCGGAAGGATG+AGG	0.559200	8.3:-36372006	MS.gene71204:CDS
GATAGTCTTTGTTCAAGTGT+GGG	0.563804	8.3:+36371218	None:intergenic
GAGTCAAAGGAAGGGAAGGT+TGG	0.564225	8.3:-36372238	MS.gene71204:CDS
TACACATCGCCGCATTTAAG+GGG	0.566105	8.3:-36372412	MS.gene71204:CDS
ACCTTGTCAGAGTCAAAGGA+AGG	0.577293	8.3:-36372247	MS.gene71204:CDS
TTAAGCTTCAAAGCAAAGGA+AGG	0.577787	8.3:+36372353	None:intergenic
TTTCTACCCCTAGAAATGCT+GGG	0.578596	8.3:-36370992	MS.gene71204:CDS
TTATGACTTACAAAATGAAG+AGG	0.585719	8.3:+36372212	None:intergenic
CAACCTCAATTACTTCATAG+AGG	0.585866	8.3:+36372486	None:intergenic
GAACGTTGACAAAGATGACT+AGG	0.588583	8.3:-36371361	MS.gene71204:CDS
GTCAGAGTCAAAGGAAGGGA+AGG	0.589674	8.3:-36372242	MS.gene71204:CDS
AGACTACACATGAACCCAGT+TGG	0.594739	8.3:+36370876	None:intergenic
CAATCTGCATTGACTCCCGT+TGG	0.595166	8.3:-36371077	MS.gene71204:CDS
TGGGTTCATGTGTAGTCTAG+TGG	0.603791	8.3:-36370871	MS.gene71204:CDS
TAGAGATTAAGAATATATTG+AGG	0.607089	8.3:-36371280	MS.gene71204:CDS
AACCTGATAAACTCCACCAA+CGG	0.617140	8.3:+36371061	None:intergenic
CAAACGATAAATGAACCCAC+TGG	0.620048	8.3:+36370843	None:intergenic
ATATAGAACACTAAATCGGA+AGG	0.624256	8.3:-36372012	MS.gene71204:CDS
GCTTTGAAGCTTAATCCGCA+AGG	0.625551	8.3:-36372344	MS.gene71204:CDS
GAACAAAACAGCATTGGACA+TGG	0.630968	8.3:-36371312	MS.gene71204:CDS
AAGTTGCAGCTGAAGAAGGT+AGG	0.633882	8.3:-36372517	MS.gene71204:CDS
AGCGCTATGTAACGAGCCAA+AGG	0.634230	8.3:-36371958	MS.gene71204:intron
CTATTGAAGACGTGACTGTG+AGG	0.637402	8.3:-36372142	MS.gene71204:CDS
CCTTGTCAGAGTCAAAGGAA+GGG	0.639046	8.3:-36372246	MS.gene71204:CDS
CATAGAACTGGCATGTGCAG+GGG	0.646801	8.3:+36370794	None:intergenic
AAATGCGGCGATGTGTAAAG+GGG	0.647457	8.3:+36372418	None:intergenic
TATTGAAGACGTGACTGTGA+GGG	0.655156	8.3:-36372141	MS.gene71204:CDS
CCGGAGATGCCCCTTAAATG+CGG	0.660525	8.3:+36372403	None:intergenic
TGAGTTGGGCTGAATCCTTG+CGG	0.670076	8.3:+36372329	None:intergenic
CCTCAATTACTTCATAGAGG+AGG	0.675416	8.3:+36372489	None:intergenic
AGTCAATGCAGATTGATAGG+TGG	0.682516	8.3:+36371085	None:intergenic
TGATTTCCCAGCATTTCTAG+GGG	0.684424	8.3:+36370986	None:intergenic
TAACTATTCTGATACCAACT+GGG	0.688002	8.3:-36370890	MS.gene71204:CDS
ATTGAAGACGTGACTGTGAG+GGG	0.711152	8.3:-36372140	MS.gene71204:CDS
CACTTGAACAAAGACTATCA+CGG	0.714741	8.3:-36371214	MS.gene71204:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAATATGAATACAAAAAATT+TGG	-	chr8.3:36371892-36371911	MS.gene71204:intron	10.0%
!!	AAGAAATATGATATATTTAA+AGG	+	chr8.3:36371578-36371597	None:intergenic	10.0%
!!	TATATATTATTTAGATTTAG+AGG	+	chr8.3:36371338-36371357	None:intergenic	10.0%
!!	TAACACTTTATATATAATCT+GGG	+	chr8.3:36372041-36372060	None:intergenic	15.0%
!!	TTAACACTTTATATATAATC+TGG	+	chr8.3:36372042-36372061	None:intergenic	15.0%
!!	TTTCAAATTCATAATTTCAA+GGG	+	chr8.3:36370855-36370874	None:intergenic	15.0%
!!	AATTCATTTGACAATAAGAA+TGG	-	chr8.3:36371810-36371829	MS.gene71204:intron	20.0%
!!	CATTAGAAATGATATATTAG+AGG	-	chr8.3:36372075-36372094	MS.gene71204:CDS	20.0%
!!	GTTTCAAATTCATAATTTCA+AGG	+	chr8.3:36370856-36370875	None:intergenic	20.0%
!!	TAGAATATAGAACACTAAAT+CGG	-	chr8.3:36371212-36371231	MS.gene71204:CDS	20.0%
!!	TAGAGATTAAGAATATATTG+AGG	-	chr8.3:36371948-36371967	MS.gene71204:intron	20.0%
!!	TTGTAGTATGTATGTAAAAA+TGG	-	chr8.3:36371631-36371650	MS.gene71204:intron	20.0%
!!	TTTGAAAAATAACAAGTTCA+AGG	-	chr8.3:36371132-36371151	MS.gene71204:CDS	20.0%
!!!	CTATATTCTCTAAAATAGAT+GGG	+	chr8.3:36370769-36370788	None:intergenic	20.0%
!!!	CTTTTAGAGTCTTAATTTAA+AGG	-	chr8.3:36371516-36371535	MS.gene71204:intron	20.0%
!!!	TCTATATTCTCTAAAATAGA+TGG	+	chr8.3:36370770-36370789	None:intergenic	20.0%
!	AACTTTCAGTTTATGAAGAT+TGG	+	chr8.3:36370696-36370715	None:intergenic	25.0%
!	ATAAGATAGTAAATGAGTGT+AGG	+	chr8.3:36371410-36371429	None:intergenic	25.0%
!	ATGTGATAGATGTAATAGTT+TGG	+	chr8.3:36371472-36371491	None:intergenic	25.0%
!	ATTAAGAAAAATGTGAAGAG+AGG	-	chr8.3:36371178-36371197	MS.gene71204:CDS	25.0%
!	GCTATAGTTGACATAAAAAA+GGG	+	chr8.3:36372316-36372335	None:intergenic	25.0%
!	TATACAACTAACTGAATTGA+AGG	+	chr8.3:36371299-36371318	None:intergenic	25.0%
!	TATGTTGAAAATCATAGAAC+TGG	+	chr8.3:36372449-36372468	None:intergenic	25.0%
!	TCAACAAAACCATAAAAGAA+TGG	-	chr8.3:36370931-36370950	MS.gene71204:CDS	25.0%
!	TGCTATAGTTGACATAAAAA+AGG	+	chr8.3:36372317-36372336	None:intergenic	25.0%
!	TTATGACTTACAAAATGAAG+AGG	+	chr8.3:36371019-36371038	None:intergenic	25.0%
!!	AAGAAAACACCATTCTTTTA+TGG	+	chr8.3:36370943-36370962	None:intergenic	25.0%
!!	TTATTTCTTTCTCTCTCTAA+TGG	+	chr8.3:36371728-36371747	None:intergenic	25.0%
!!!	GTTCATTTGTTAACTTTTGT+CGG	-	chr8.3:36371653-36371672	MS.gene71204:intron	25.0%
	AAGAATATATTGAGGAATGC+TGG	-	chr8.3:36371956-36371975	MS.gene71204:intron	30.0%
	ATATAGAACACTAAATCGGA+AGG	-	chr8.3:36371216-36371235	MS.gene71204:CDS	30.0%
	CAATTAGGAACTTAGCTAAA+AGG	+	chr8.3:36371054-36371073	None:intergenic	30.0%
	GGTAGAAATTGTAGAATTAG+AGG	+	chr8.3:36372224-36372243	None:intergenic	30.0%
	TAACTATTCTGATACCAACT+GGG	-	chr8.3:36372338-36372357	MS.gene71204:CDS	30.0%
	TTCAGTTTATGAAGATTGGT+TGG	+	chr8.3:36370692-36370711	None:intergenic	30.0%
!	AGAATGGTGTTTTCTTTTGT+CGG	-	chr8.3:36370947-36370966	MS.gene71204:CDS	30.0%
!	TCAGTTTATGAAGATTGGTT+GGG	+	chr8.3:36370691-36370710	None:intergenic	30.0%
!!	CGATAAGTTGATGATATGAT+AGG	-	chr8.3:36371768-36371787	MS.gene71204:intron	30.0%
	AACAACTTTCTTGTTAGCAC+AGG	-	chr8.3:36371838-36371857	MS.gene71204:intron	35.0%
	AATAACCTTGTCAGAGTCAA+AGG	-	chr8.3:36370977-36370996	MS.gene71204:CDS	35.0%
	ACTGAATTGAAGGTTACCTT+TGG	+	chr8.3:36371289-36371308	None:intergenic	35.0%
	CAACCTCAATTACTTCATAG+AGG	+	chr8.3:36370745-36370764	None:intergenic	35.0%
	CACTTGAACAAAGACTATCA+CGG	-	chr8.3:36372014-36372033	MS.gene71204:CDS	35.0%
	CTGAAGCTACAACATAGTTA+AGG	-	chr8.3:36371676-36371695	MS.gene71204:intron	35.0%
	GATAGTCTTTGTTCAAGTGT+GGG	+	chr8.3:36372013-36372032	None:intergenic	35.0%
	GTAACTATTCTGATACCAAC+TGG	-	chr8.3:36372337-36372356	MS.gene71204:CDS	35.0%
	TAGAATCAGGACAAGCAATT+AGG	+	chr8.3:36371069-36371088	None:intergenic	35.0%
	TCATAATTTCAAGGGCAAAC+CGG	+	chr8.3:36370847-36370866	None:intergenic	35.0%
	TGATAGTCTTTGTTCAAGTG+TGG	+	chr8.3:36372014-36372033	None:intergenic	35.0%
	TGTCAAATGAATTCTTTCGC+TGG	+	chr8.3:36371803-36371822	None:intergenic	35.0%
	TTAAGCTTCAAAGCAAAGGA+AGG	+	chr8.3:36370878-36370897	None:intergenic	35.0%
	TTCTCTCTCTAATGGTAGAT+CGG	+	chr8.3:36371720-36371739	None:intergenic	35.0%
	TTGATTTCCCAGCATTTCTA+GGG	+	chr8.3:36372246-36372265	None:intergenic	35.0%
	TTTGGAGAACAAAACAGCAT+TGG	-	chr8.3:36371910-36371929	MS.gene71204:intron	35.0%
!	ATTGATTTCCCAGCATTTCT+AGG	+	chr8.3:36372247-36372266	None:intergenic	35.0%
!	GTTATCTCTTTTCTATGTCG+AGG	-	chr8.3:36372407-36372426	MS.gene71204:CDS	35.0%
	AACACTAAATCGGAAGGATG+AGG	-	chr8.3:36371222-36371241	MS.gene71204:CDS	40.0%
	AACCTGATAAACTCCACCAA+CGG	+	chr8.3:36372170-36372189	None:intergenic	40.0%
	AACTTTCTTGTTAGCACAGG+TGG	-	chr8.3:36371841-36371860	MS.gene71204:intron	40.0%
	AATGTTGTGTACACCCTATG+TGG	+	chr8.3:36371553-36371572	None:intergenic	40.0%
	AGTCAATGCAGATTGATAGG+TGG	+	chr8.3:36372146-36372165	None:intergenic	40.0%
	ATGTTGTGTACACCCTATGT+GGG	+	chr8.3:36371552-36371571	None:intergenic	40.0%
	ATTTCTACCCCTAGAAATGC+TGG	-	chr8.3:36372235-36372254	MS.gene71204:CDS	40.0%
	CAAACGATAAATGAACCCAC+TGG	+	chr8.3:36372388-36372407	None:intergenic	40.0%
	CCTCAATTACTTCATAGAGG+AGG	+	chr8.3:36370742-36370761	None:intergenic	40.0%
	CCTCCTCTATGAAGTAATTG+AGG	-	chr8.3:36370739-36370758	MS.gene71204:CDS	40.0%
	CGGATTAAGCTTCAAAGCAA+AGG	+	chr8.3:36370882-36370901	None:intergenic	40.0%
	GAACGTTGACAAAGATGACT+AGG	-	chr8.3:36371867-36371886	MS.gene71204:intron	40.0%
	GCTGGGAAATCAATATTGTC+AGG	-	chr8.3:36372253-36372272	MS.gene71204:CDS	40.0%
	GTCACGTCTTCAATAGAATC+AGG	+	chr8.3:36371082-36371101	None:intergenic	40.0%
	GTTGAATAGCTACGTGAGTT+GGG	+	chr8.3:36370916-36370935	None:intergenic	40.0%
	TAAATGCGGCGATGTGTAAA+GGG	+	chr8.3:36370814-36370833	None:intergenic	40.0%
	TATTGAAGACGTGACTGTGA+GGG	-	chr8.3:36371087-36371106	MS.gene71204:CDS	40.0%
	TGTTGAATAGCTACGTGAGT+TGG	+	chr8.3:36370917-36370936	None:intergenic	40.0%
	TTAAATGCGGCGATGTGTAA+AGG	+	chr8.3:36370815-36370834	None:intergenic	40.0%
	TTACACATCGCCGCATTTAA+GGG	-	chr8.3:36370815-36370834	MS.gene71204:CDS	40.0%
	TTTACACATCGCCGCATTTA+AGG	-	chr8.3:36370814-36370833	MS.gene71204:CDS	40.0%
	TTTCTACCCCTAGAAATGCT+GGG	-	chr8.3:36372236-36372255	MS.gene71204:CDS	40.0%
!	GAACAAAACAGCATTGGACA+TGG	-	chr8.3:36371916-36371935	MS.gene71204:intron	40.0%
!	TGATTTCCCAGCATTTCTAG+GGG	+	chr8.3:36372245-36372264	None:intergenic	40.0%
	AAATGCGGCGATGTGTAAAG+GGG	+	chr8.3:36370813-36370832	None:intergenic	45.0%
	AAGTTGCAGCTGAAGAAGGT+AGG	-	chr8.3:36370711-36370730	MS.gene71204:CDS	45.0%
	ACCTGATAAACTCCACCAAC+GGG	+	chr8.3:36372169-36372188	None:intergenic	45.0%
	ACCTTGTCAGAGTCAAAGGA+AGG	-	chr8.3:36370981-36371000	MS.gene71204:CDS	45.0%
	AGACTACACATGAACCCAGT+TGG	+	chr8.3:36372355-36372374	None:intergenic	45.0%
	ATCATAGAACTGGCATGTGC+AGG	+	chr8.3:36372439-36372458	None:intergenic	45.0%
	ATTGAAGACGTGACTGTGAG+GGG	-	chr8.3:36371088-36371107	MS.gene71204:CDS	45.0%
	CCTTGTCAGAGTCAAAGGAA+GGG	-	chr8.3:36370982-36371001	MS.gene71204:CDS	45.0%
	CTATTGAAGACGTGACTGTG+AGG	-	chr8.3:36371086-36371105	MS.gene71204:CDS	45.0%
	CTGAAAGTTGCAGCTGAAGA+AGG	-	chr8.3:36370707-36370726	MS.gene71204:CDS	45.0%
	GGGAGTCAATGCAGATTGAT+AGG	+	chr8.3:36372149-36372168	None:intergenic	45.0%
	TACACATCGCCGCATTTAAG+GGG	-	chr8.3:36370816-36370835	MS.gene71204:CDS	45.0%
	TAGAGGAGCAACGTAACTCT+TGG	-	chr8.3:36372092-36372111	MS.gene71204:CDS	45.0%
	TCATAGAACTGGCATGTGCA+GGG	+	chr8.3:36372438-36372457	None:intergenic	45.0%
	TCCTTCATGAACGCATTGCT+CGG	-	chr8.3:36372527-36372546	MS.gene71204:CDS	45.0%
	TGGGTTCATGTGTAGTCTAG+TGG	-	chr8.3:36372357-36372376	MS.gene71204:CDS	45.0%
!!	GCTTTGAAGCTTAATCCGCA+AGG	-	chr8.3:36370884-36370903	MS.gene71204:CDS	45.0%
	AGCGCTATGTAACGAGCCAA+AGG	-	chr8.3:36371270-36371289	MS.gene71204:CDS	50.0%
	AGGTGTGTACACACCCACAT+AGG	-	chr8.3:36371536-36371555	MS.gene71204:intron	50.0%
	CATAGAACTGGCATGTGCAG+GGG	+	chr8.3:36372437-36372456	None:intergenic	50.0%
	CCCTTCCTTTGACTCTGACA+AGG	+	chr8.3:36370985-36371004	None:intergenic	50.0%
	GAGTCAAAGGAAGGGAAGGT+TGG	-	chr8.3:36370990-36371009	MS.gene71204:CDS	50.0%
	GGTGTGTACACACCCACATA+GGG	-	chr8.3:36371537-36371556	MS.gene71204:intron	50.0%
	GTCAGAGTCAAAGGAAGGGA+AGG	-	chr8.3:36370986-36371005	MS.gene71204:CDS	50.0%
	TCCGAGCAATGCGTTCATGA+AGG	+	chr8.3:36372531-36372550	None:intergenic	50.0%
!	GCATCGATGACTTGTGAGCT+AGG	+	chr8.3:36371989-36372008	None:intergenic	50.0%
!	TCCCGTTGGTGGAGTTTATC+AGG	-	chr8.3:36372165-36372184	MS.gene71204:CDS	50.0%
!	TGAGTTGGGCTGAATCCTTG+CGG	+	chr8.3:36370902-36370921	None:intergenic	50.0%
!!	CAATCTGCATTGACTCCCGT+TGG	-	chr8.3:36372151-36372170	MS.gene71204:CDS	50.0%
	AGTCTAGTGGCAGCTCCAGT+GGG	-	chr8.3:36372370-36372389	MS.gene71204:CDS	55.0%
	CCGGAGATGCCCCTTAAATG+CGG	+	chr8.3:36370828-36370847	None:intergenic	55.0%
	TAGTCTAGTGGCAGCTCCAG+TGG	-	chr8.3:36372369-36372388	MS.gene71204:CDS	55.0%
!!	CCGCATTTAAGGGGCATCTC+CGG	-	chr8.3:36370825-36370844	MS.gene71204:CDS	55.0%
!!	TCTGCATTGACTCCCGTTGG+TGG	-	chr8.3:36372154-36372173	MS.gene71204:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.3	gene	36370683	36372567	36370683	ID=MS.gene71204
chr8.3	mRNA	36370683	36372567	36370683	ID=MS.gene71204.t1;Parent=MS.gene71204
chr8.3	exon	36371959	36372567	36371959	ID=MS.gene71204.t1.exon1;Parent=MS.gene71204.t1
chr8.3	CDS	36371959	36372567	36371959	ID=cds.MS.gene71204.t1;Parent=MS.gene71204.t1
chr8.3	exon	36370683	36371390	36370683	ID=MS.gene71204.t1.exon2;Parent=MS.gene71204.t1
chr8.3	CDS	36370683	36371390	36370683	ID=cds.MS.gene71204.t1;Parent=MS.gene71204.t1

Gene Sequence

>MS.gene71204

ATGAACCCAACCAATCTTCATAAACTGAAAGTTGCAGCTGAAGAAGGTAGGATAGACCTCCTCTATGAAGTAATTGAGGTTGACCCATCTATTTTAGAGAATATAGATTCAATACAGTTTGTCGAAACCCCTTTACACATCGCCGCATTTAAGGGGCATCTCCGGTTTGCCCTTGAAATTATGAATTTGAAACCTTCCTTTGCTTTGAAGCTTAATCCGCAAGGATTCAGCCCAACTCACGTAGCTATTCAACAAAACCATAAAAGAATGGTGTTTTCTTTTGTCGGCATGAACAATAACCTTGTCAGAGTCAAAGGAAGGGAAGGTTGGACTCCTCTTCATTTTGTAAGTCATAATGAAGAAGTTGACCTTTTAGCTAAGTTCCTAATTGCTTGTCCTGATTCTATTGAAGACGTGACTGTGAGGGGTGAGACTGCTCTACATATTGCTTTGAAAAATAACAAGTTCAAGGCTCTTGATCTTCTTGTTTGTTTTATTAAGAAAAATGTGAAGAGAGGTGCTAGAAAGTTAGAATATAGAACACTAAATCGGAAGGATGAGGATGACAACACTATTTTGCACATTTCAGCGCTATGTAACGAGCCAAAGGTAACCTTCAATTCAGTTAGTTGTATAGATATATTTTTGCTTTCCTCTAAATCTAAATAATATATATGTTGTGATCGTAAATATCAAACAATGATATTTGAACACATCAATTTTTCCTACACTCATTTACTATCTTATTTTCTCTATATCATTTTATTTCTAAATTCTATCACATCACCAAACTATTACATCTATCACATCACTCTTTTTTTCTCTTTTTTTCTCTTTTAGAGTCTTAATTTAAAGGTGTGTACACACCCACATAGGGTGTACACAACATTTTCCTTTAAATATATCATATTTCTTAACTGTTAATTTCTAAGTGTATGACTAATTAATTTGTAGTATGTATGTAAAAATGGTTCATTTGTTAACTTTTGTCGGCTGAAGCTACAACATAGTTAAGGTAAATAATTCTTAGACATCCGATCTACCATTAGAGAGAGAAAGAAATAAATAGAGTAAAGTGCTAGAAGCGATAAGTTGATGATATGATAGGAAAAGACTACCAGCGAAAGAATTCATTTGACAATAAGAATGGTCTCTAACAACTTTCTTGTTAGCACAGGTGGTTCGAACGTTGACAAAGATGACTAGGATAAATATGAATACAAAAAATTTGGAGAACAAAACAGCATTGGACATGGCAGTCAATGTAGAGATTAAGAATATATTGAGGAATGCTGGAGCAAAACCTAGCTCACAAGTCATCGATGCTCCCACACTTGAACAAAGACTATCACGGACCCAGATTATATATAAAGTGTTAACTTCTTTATGTCGCATTAGAAATGATATATTAGAGGAGCAACGTAACTCTTGGATGATAGTTGCGACTCTTGTTGCAACAGCCACCTATCAATCTGCATTGACTCCCGTTGGTGGAGTTTATCAGGTTAATGCTAGTGACAATACTGTCAACATTACTTCCTCTAATTCTACAATTTCTACCCCTAGAAATGCTGGGAAATCAATATTGTCAGGAGATGATTTCTTTATATTTTTAATTTCGAATATGCTTCCCTTTTTTATGTCAACTATAGCAGTAACTATTCTGATACCAACTGGGTTCATGTGTAGTCTAGTGGCAGCTCCAGTGGGTTCATTTATCGTTTGTTATCTCTTTTCTATGTCGAGGATATCCCCTGCACATGCCAGTTCTATGATTTTCAACATAGTTTTGAGTTCAATTGTGTTTGTTGAAGCTATTTTGCTCATTTTCATAGTGTATTCTATCCTTCATGAACGCATTGCTCGGAAATTGCGTAGATCATAG

Protein sequence

>MS.gene71204.t1

MNPTNLHKLKVAAEEGRIDLLYEVIEVDPSILENIDSIQFVETPLHIAAFKGHLRFALEIMNLKPSFALKLNPQGFSPTHVAIQQNHKRMVFSFVGMNNNLVRVKGREGWTPLHFVSHNEEVDLLAKFLIACPDSIEDVTVRGETALHIALKNNKFKALDLLVCFIKKNVKRGARKLEYRTLNRKDEDDNTILHISALCNEPKVVRTLTKMTRINMNTKNLENKTALDMAVNVEIKNILRNAGAKPSSQVIDAPTLEQRLSRTQIIYKVLTSLCRIRNDILEEQRNSWMIVATLVATATYQSALTPVGGVYQVNASDNTVNITSSNSTISTPRNAGKSILSGDDFFIFLISNMLPFFMSTIAVTILIPTGFMCSLVAAPVGSFIVCYLFSMSRISPAHASSMIFNIVLSSIVFVEAILLIFIVYSILHERIARKLRRS