AlfalfaGEDB | MS.gene97943

M. sativa cultivar XinJiangDaYe / MS.gene97943

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF12796.10 PF13857.9 PF13962.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene97943.t1	XP_013446189.1	82.6	465	79	1	1	463	1	465	7.00E-178	633.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene97943.t1	Q8GYH5	31.3	393	236	5	12	393	10	379	2.4e-17	91.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene97943.t1	A0A072TSN3	82.6	465	79	1	1	463	1	465	5.0e-178	633.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene97943	MS.gene014931	PPI
MS.gene97943	MS.gene062662	PPI
MS.gene97943	MS.gene21888	PPI
MS.gene97943	MS.gene97379	PPI
MS.gene97943	MS.gene066500	PPI
MS.gene005995	MS.gene97943	PPI
MS.gene037950	MS.gene97943	PPI
MS.gene97943	MS.gene008767	PPI
MS.gene97943	MS.gene39623	PPI
MS.gene97943	MS.gene066464	PPI
MS.gene97943	MS.gene07706	PPI
MS.gene97943	MS.gene071113	PPI
MS.gene97943	MS.gene90400	PPI
MS.gene24806	MS.gene97943	PPI
MS.gene97943	MS.gene022109	PPI
MS.gene69687	MS.gene97943	PPI
MS.gene97943	MS.gene041770	PPI
MS.gene97943	MS.gene81055	PPI
MS.gene97943	MS.gene65775	PPI
MS.gene97943	MS.gene99554	PPI
MS.gene97943	MS.gene80163	PPI
MS.gene97943	MS.gene02469	PPI
MS.gene97943	MS.gene33377	PPI
MS.gene066464	MS.gene97943	PPI
MS.gene97943	MS.gene51335	PPI
MS.gene97943	MS.gene045937	PPI
MS.gene97943	MS.gene027072	PPI
MS.gene97943	MS.gene51278	PPI
MS.gene97943	MS.gene69966	PPI
MS.gene97943	MS.gene87218	PPI
MS.gene010912	MS.gene97943	PPI
MS.gene97943	MS.gene025229	PPI
MS.gene97943	MS.gene30433	PPI
MS.gene97943	MS.gene066501	PPI
MS.gene97943	MS.gene043742	PPI
MS.gene51335	MS.gene97943	PPI
MS.gene97943	MS.gene027441	PPI
MS.gene97943	MS.gene21734	PPI
MS.gene97943	MS.gene30177	PPI
MS.gene36842	MS.gene97943	PPI
MS.gene97943	MS.gene70008	PPI
MS.gene97943	MS.gene008833	PPI
MS.gene068249	MS.gene97943	PPI
MS.gene97943	MS.gene23811	PPI
MS.gene97943	MS.gene25244	PPI
MS.gene97943	MS.gene82740	PPI
MS.gene97943	MS.gene051003	PPI
MS.gene97943	MS.gene48655	PPI
MS.gene97943	MS.gene09785	PPI
MS.gene97943	MS.gene64441	PPI
MS.gene97943	MS.gene066046	PPI
MS.gene97943	MS.gene007486	PPI
MS.gene97943	MS.gene23810	PPI
MS.gene97943	MS.gene08470	PPI
MS.gene97943	MS.gene027073	PPI
MS.gene97943	MS.gene34712	PPI
MS.gene97943	MS.gene65463	PPI
MS.gene97943	MS.gene007485	PPI
MS.gene039682	MS.gene97943	PPI
MS.gene97943	MS.gene007487	PPI
MS.gene97943	MS.gene037184	PPI
MS.gene26532	MS.gene97943	PPI
MS.gene97943	MS.gene23196	PPI
MS.gene47479	MS.gene97943	PPI
MS.gene97943	MS.gene067705	PPI
MS.gene97943	MS.gene71204	PPI
MS.gene97943	MS.gene41291	PPI
MS.gene71204	MS.gene97943	PPI
MS.gene97943	MS.gene027075	PPI
MS.gene97943	MS.gene061932	PPI
MS.gene97943	MS.gene050457	PPI
MS.gene97943	MS.gene99973	PPI
MS.gene97943	MS.gene30174	PPI
MS.gene97943	MS.gene26021	PPI
MS.gene97943	MS.gene064542	PPI
MS.gene066501	MS.gene97943	PPI
MS.gene97943	MS.gene91775	PPI
MS.gene97943	MS.gene25515	PPI
MS.gene056455	MS.gene97943	PPI
MS.gene97943	MS.gene34651	PPI
MS.gene97943	MS.gene89986	PPI
MS.gene97943	MS.gene22482	PPI
MS.gene97943	MS.gene050270	PPI
MS.gene97943	MS.gene052956	PPI
MS.gene035805	MS.gene97943	PPI
MS.gene97943	MS.gene60920	PPI
MS.gene97943	MS.gene058128	PPI
MS.gene69965	MS.gene97943	PPI
MS.gene97943	MS.gene34009	PPI
MS.gene97943	MS.gene018518	PPI
MS.gene97943	MS.gene043662	PPI
MS.gene97943	MS.gene065848	PPI
MS.gene97943	MS.gene004844	PPI
MS.gene97943	MS.gene068249	PPI
MS.gene97943	MS.gene99588	PPI
MS.gene97943	MS.gene54764	PPI
MS.gene97943	MS.gene038519	PPI
MS.gene97943	MS.gene27223	PPI
MS.gene97943	MS.gene042783	PPI
MS.gene30172	MS.gene97943	PPI
MS.gene97943	MS.gene25024	PPI
MS.gene97943	MS.gene012203	PPI
MS.gene23852	MS.gene97943	PPI
MS.gene97943	MS.gene26019	PPI
MS.gene071113	MS.gene97943	PPI
MS.gene97943	MS.gene050272	PPI
MS.gene97943	MS.gene63085	PPI
MS.gene027071	MS.gene97943	PPI
MS.gene67157	MS.gene97943	PPI
MS.gene97943	MS.gene050456	PPI
MS.gene97943	MS.gene30176	PPI
MS.gene97943	MS.gene51258	PPI
MS.gene97943	MS.gene32723	PPI
MS.gene97943	MS.gene053363	PPI
MS.gene010910	MS.gene97943	PPI
MS.gene97943	MS.gene066467	PPI
MS.gene97943	MS.gene024753	PPI
MS.gene063222	MS.gene97943	PPI
MS.gene07706	MS.gene97943	PPI
MS.gene027074	MS.gene97943	PPI
MS.gene97943	MS.gene32726	PPI
MS.gene97943	MS.gene71122	PPI
MS.gene97943	MS.gene24922	PPI
MS.gene97943	MS.gene73119	PPI
MS.gene97943	MS.gene92243	PPI
MS.gene97943	MS.gene85534	PPI
MS.gene97943	MS.gene037950	PPI
MS.gene97943	MS.gene51262	PPI
MS.gene23811	MS.gene97943	PPI
MS.gene97943	MS.gene23881	PPI
MS.gene23810	MS.gene97943	PPI
MS.gene97943	MS.gene26672	PPI
MS.gene78281	MS.gene97943	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene97943.t1	MTR_8g069880	91.828	465	36	1	1	463	1	465	0.0	886
MS.gene97943.t1	MTR_8g072510	57.363	455	171	6	3	452	6	442	1.36e-165	474
MS.gene97943.t1	MTR_8g467780	54.810	447	186	5	7	441	11	453	6.86e-151	437
MS.gene97943.t1	MTR_8g039620	55.916	431	183	5	3	428	10	438	5.28e-150	434
MS.gene97943.t1	MTR_8g062410	55.213	422	180	3	3	422	6	420	6.01e-149	432
MS.gene97943.t1	MTR_3g016210	56.968	409	160	4	3	395	6	414	3.26e-148	430
MS.gene97943.t1	MTR_7g021090	58.568	391	150	8	3	388	5	388	1.16e-141	412
MS.gene97943.t1	MTR_8g040930	56.499	377	154	3	50	416	2	378	1.22e-138	403
MS.gene97943.t1	MTR_8g041240	56.499	377	154	3	50	416	2	378	1.22e-138	403
MS.gene97943.t1	MTR_8g068990	58.491	371	137	4	10	377	15	371	7.27e-138	404
MS.gene97943.t1	MTR_8g073260	54.612	412	177	6	1	405	4	412	1.07e-137	403
MS.gene97943.t1	MTR_8g066670	54.462	437	194	5	2	436	1070	1503	7.23e-134	421
MS.gene97943.t1	MTR_1g059930	60.857	350	134	3	7	354	12	360	2.18e-130	384
MS.gene97943.t1	MTR_7g451420	65.161	310	106	2	2	310	4	312	1.16e-129	378
MS.gene97943.t1	MTR_3g105650	40.233	430	209	8	5	423	3	395	5.22e-96	296
MS.gene97943.t1	MTR_8g073275	44.444	414	151	10	50	395	2	404	1.07e-95	296
MS.gene97943.t1	MTR_1g069110	26.709	468	288	12	10	452	21	458	1.21e-37	143
MS.gene97943.t1	MTR_7g099580	25.333	300	173	6	16	310	21	274	1.84e-23	101
MS.gene97943.t1	MTR_2g101320	28.794	257	153	7	32	269	131	376	8.73e-22	99.0
MS.gene97943.t1	MTR_2g101310	30.366	191	121	3	38	227	137	316	5.93e-18	87.0
MS.gene97943.t1	MTR_7g117620	25.080	311	189	6	26	306	97	393	1.18e-17	85.9
MS.gene97943.t1	MTR_7g099570	27.626	257	160	6	7	247	9	255	1.46e-17	85.9
MS.gene97943.t1	MTR_2g101310	30.366	191	121	3	38	227	137	316	2.14e-17	85.1
MS.gene97943.t1	MTR_1g115575	23.343	347	206	9	42	363	169	480	2.76e-17	84.7
MS.gene97943.t1	MTR_1g032100	22.564	390	233	10	6	363	59	411	1.03e-14	76.6
MS.gene97943.t1	MTR_4g062100	24.217	351	214	11	7	306	35	384	3.44e-13	72.0
MS.gene97943.t1	MTR_7g100430	25.538	325	186	11	34	307	118	437	3.55e-13	72.0
MS.gene97943.t1	MTR_7g100390	23.000	300	191	5	43	318	88	371	5.27e-12	68.2
MS.gene97943.t1	MTR_8g105260	21.779	326	208	6	11	306	70	378	2.41e-11	66.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene97943.t1	AT4G10720	37.915	422	226	9	10	411	8	413	1.05e-82	262
MS.gene97943.t1	AT4G10720	36.603	418	204	8	10	411	8	380	1.12e-76	246
MS.gene97943.t1	AT5G15500	35.281	445	251	10	7	436	6	428	1.55e-76	246
MS.gene97943.t1	AT1G14500	34.321	405	223	6	7	394	5	383	2.99e-76	245
MS.gene97943.t1	AT1G14480	34.676	447	251	8	7	439	5	424	2.03e-74	241
MS.gene97943.t1	AT5G54620	37.306	386	211	4	12	389	10	372	3.60e-74	239
MS.gene97943.t1	AT5G54610	32.000	375	225	4	12	379	10	361	2.11e-60	203
MS.gene97943.t1	AT1G14480	31.320	447	223	10	7	439	5	381	2.18e-53	184
MS.gene97943.t1	AT5G15500	31.395	344	199	10	108	436	1	322	2.03e-40	148
MS.gene97943.t1	AT5G51160	27.907	387	218	13	61	410	35	397	8.64e-32	126
MS.gene97943.t1	AT4G03500	29.954	217	138	5	92	307	276	479	9.53e-19	89.7
MS.gene97943.t1	AT4G03500	29.954	217	138	5	92	307	276	479	9.53e-19	89.7
MS.gene97943.t1	AT4G03500	29.954	217	138	5	92	307	299	502	9.69e-19	89.7
MS.gene97943.t1	AT4G03460	30.702	228	121	6	92	307	252	454	5.91e-18	87.0
MS.gene97943.t1	AT4G03460	30.702	228	121	6	92	307	284	486	6.29e-18	87.0
MS.gene97943.t1	AT5G54700	25.269	372	203	13	93	446	17	331	1.80e-17	84.0
MS.gene97943.t1	AT3G09550	23.698	384	222	11	10	363	157	499	4.31e-17	84.3
MS.gene97943.t1	AT3G09550	23.698	384	222	11	10	363	150	492	4.53e-17	84.3
MS.gene97943.t1	AT3G09550	23.698	384	222	11	10	363	150	492	4.53e-17	84.3
MS.gene97943.t1	AT1G10340	23.750	400	233	12	77	430	175	548	5.20e-17	84.0
MS.gene97943.t1	AT1G10340	23.750	400	233	12	77	430	171	544	5.21e-17	84.0
MS.gene97943.t1	AT5G54700	24.348	345	174	8	22	364	20	279	1.77e-16	82.0
MS.gene97943.t1	AT2G24600	24.010	404	227	14	77	430	175	548	2.67e-16	82.0
MS.gene97943.t1	AT2G24600	24.010	404	227	14	77	430	175	548	2.67e-16	82.0
MS.gene97943.t1	AT1G03670	29.386	228	109	8	99	307	279	473	2.93e-16	81.6
MS.gene97943.t1	AT1G34050	23.678	397	243	11	63	430	174	539	3.94e-16	81.3
MS.gene97943.t1	AT2G24600	26.866	268	152	7	77	310	175	432	5.90e-16	80.9
MS.gene97943.t1	AT2G24600	26.866	268	152	7	77	310	175	432	5.90e-16	80.9
MS.gene97943.t1	AT3G12360	26.074	349	176	13	22	307	114	443	8.33e-16	80.5
MS.gene97943.t1	AT3G12360	26.074	349	176	13	22	307	102	431	1.01e-15	80.1
MS.gene97943.t1	AT2G31820	26.154	325	187	13	19	306	193	501	1.11e-15	80.1
MS.gene97943.t1	AT2G01680	23.560	382	226	12	13	363	68	414	2.15e-15	79.0
MS.gene97943.t1	AT5G54710	25.938	320	158	11	21	310	181	451	2.63e-15	79.0
MS.gene97943.t1	AT5G54710	25.938	320	158	11	21	310	226	496	2.90e-15	78.6
MS.gene97943.t1	AT4G03480	24.211	285	179	8	58	327	372	634	3.04e-13	72.4
MS.gene97943.t1	AT4G03480	24.643	280	172	8	58	322	375	630	3.19e-13	72.4
MS.gene97943.t1	AT1G05640	23.513	353	183	10	40	306	114	465	6.62e-13	71.2
MS.gene97943.t1	AT5G02620	27.273	187	122	3	43	228	95	268	1.38e-12	70.1
MS.gene97943.t1	AT5G02620	27.273	187	122	3	43	228	95	268	1.38e-12	70.1
MS.gene97943.t1	AT5G02620	27.273	187	122	3	43	228	95	268	1.38e-12	70.1
MS.gene97943.t1	AT4G03490	23.988	346	160	12	41	307	146	467	7.61e-12	68.2
MS.gene97943.t1	AT4G03490	23.988	346	160	12	41	307	136	457	7.72e-12	67.8
MS.gene97943.t1	AT4G03490	23.988	346	160	12	41	307	146	467	8.16e-12	68.2
MS.gene97943.t1	AT1G07710	23.676	321	207	7	10	306	73	379	3.09e-11	65.9
MS.gene97943.t1	AT1G07710	23.676	321	207	7	10	306	73	379	3.09e-11	65.9

Find 87 sgRNAs with CRISPR-Local

Find 113 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTCAGAAGTTGATTTCTTTC+GGG	0.234667	8.4:-33566092	MS.gene97943:CDS
AAGGACCTTGTCAAAGTTAA+AGG	0.247391	8.4:-33566963	MS.gene97943:CDS
AAAGGTCAATCTCTCCAATT+TGG	0.255210	8.4:+33566907	None:intergenic
TGTCAGAAGTTGATTTCTTT+CGG	0.264542	8.4:-33566093	MS.gene97943:CDS
CTTCATTATGCAAGCCAAAT+TGG	0.271372	8.4:-33566921	MS.gene97943:CDS
TCTATCTCCCTCAAAATAGA+TGG	0.305208	8.4:+33567173	None:intergenic
GGTAAAGGACCTAGAAAATA+TGG	0.309999	8.4:-33565750	MS.gene97943:CDS
ATCACTGTCTTGATTATTGC+GGG	0.317234	8.4:-33565866	MS.gene97943:CDS
TGTTGCCTGCTACATCCTTC+TGG	0.332367	8.4:+33566709	None:intergenic
TAGGGTAGAAATAGTAGAAT+TGG	0.356230	8.4:+33566138	None:intergenic
TTGCATATCGCCGCATTTAA+CGG	0.361953	8.4:-33567125	MS.gene97943:CDS
CCTTGAGAAAATAAGCTAAA+AGG	0.376196	8.4:+33566889	None:intergenic
TACTGCAATTGACATCAAAA+AGG	0.383470	8.4:+33566048	None:intergenic
ATTTCTTTCGGGTTTCATAT+TGG	0.387412	8.4:-33566081	MS.gene97943:CDS
TTTAACTCCAACTGGAAAAG+AGG	0.387649	8.4:-33566017	MS.gene97943:CDS
TTTCAAATTCATAACTTCAA+TGG	0.390293	8.4:+33567088	None:intergenic
GCGCATTACTAATAGCAATA+TGG	0.394004	8.4:+33566817	None:intergenic
AATAATCAAGACAGTGATAC+TGG	0.397084	8.4:+33565871	None:intergenic
CCTAGGAAATGCTGGAAAAT+CGG	0.405154	8.4:-33566119	MS.gene97943:CDS
GTCAAGTATTCAATAGATTC+AGG	0.406411	8.4:+33566861	None:intergenic
TTCATAACTTCAATGGCAAA+AGG	0.408215	8.4:+33567095	None:intergenic
TTTGAAGACAAAGAACTTTG+AGG	0.425247	8.4:-33566467	MS.gene97943:CDS
ATATTACCCGCTTCAGCTGC+AGG	0.439778	8.4:+33567227	None:intergenic
TTTGCATGGCAAGGTGGATA+GGG	0.440017	8.4:+33567027	None:intergenic
AACAACCATGGCTGTGGTAA+AGG	0.443309	8.4:-33565765	MS.gene97943:CDS
AGTATATCAAGATTGGTCAT+TGG	0.445141	8.4:+33567254	None:intergenic
GGGACTTAGTACTGATTGAT+AGG	0.446579	8.4:+33566216	None:intergenic
AACTAAGAAGATCAGTGTTA+AGG	0.448931	8.4:-33565808	MS.gene97943:CDS
CATAGAATATAGATAACTAA+TGG	0.452093	8.4:+33565961	None:intergenic
GGTAGAAATAGTAGAATTGG+AGG	0.454556	8.4:+33566141	None:intergenic
TTTGAACTTAGTTTATATGC+AGG	0.469966	8.4:+33566349	None:intergenic
TGAATCTATTGAATACTTGA+CGG	0.472640	8.4:-33566859	MS.gene97943:CDS
GCTTTCAAGCTAAATAAGAA+AGG	0.474328	8.4:-33567056	MS.gene97943:CDS
AGGACCTTGTCAAAGTTAAA+GGG	0.480217	8.4:-33566962	MS.gene97943:CDS
ATTTCTACCCTAGGAAATGC+TGG	0.480779	8.4:-33566127	MS.gene97943:CDS
TGACAGTTTATCAATAATAG+TGG	0.484355	8.4:+33566324	None:intergenic
TTTAGCTTGAAAGCAAATGA+AGG	0.490253	8.4:+33567065	None:intergenic
CCCTAAAAGTACTAGTTGGC+TGG	0.491416	8.4:-33566785	MS.gene97943:CDS
CTTAGACCTGCAGCTGAAGC+GGG	0.492988	8.4:-33567233	MS.gene97943:CDS
CTAATGGCAAACCAAGTCAC+TGG	0.500982	8.4:+33565977	None:intergenic
AAAAGTACTAGTTGGCTGGC+TGG	0.501290	8.4:-33566781	MS.gene97943:CDS
TTAACTCCAACTGGAAAAGA+GGG	0.507555	8.4:-33566016	MS.gene97943:CDS
AATTCTACTATTTCTACCCT+AGG	0.517193	8.4:-33566136	MS.gene97943:CDS
CAATGTACATCCCAGTGACT+TGG	0.520189	8.4:-33565988	MS.gene97943:CDS
CCTTCCCTTTAACTTTGACA+AGG	0.523374	8.4:+33566958	None:intergenic
TAGGTCCTTTACCACAGCCA+TGG	0.526478	8.4:+33565760	None:intergenic
AGGTCTAAGTATATCAAGAT+TGG	0.530471	8.4:+33567247	None:intergenic
TCATAACTTCAATGGCAAAA+GGG	0.531318	8.4:+33567096	None:intergenic
CAGGACAGGTGCTATAGAGC+TGG	0.534380	8.4:-33566748	MS.gene97943:CDS
CTATCTCCCTCAAAATAGAT+GGG	0.536487	8.4:+33567174	None:intergenic
CAAGTCACTGGGATGTACAT+TGG	0.540352	8.4:+33565989	None:intergenic
ACACGGCATTGAAATGTGTA+GGG	0.543366	8.4:+33565927	None:intergenic
GAAGATCAGTGTTAAGGCAT+CGG	0.551823	8.4:-33565802	MS.gene97943:CDS
GTCAAAGTTAAAGGGAAGGA+AGG	0.554942	8.4:-33566954	MS.gene97943:CDS
AGCATTAGACATAGCAACTA+CGG	0.556154	8.4:-33566437	MS.gene97943:CDS
CTTCAATGGCAAAAGGGAGA+TGG	0.558271	8.4:+33567102	None:intergenic
TATCACTGTCTTGATTATTG+CGG	0.560106	8.4:-33565867	MS.gene97943:CDS
ACTTAGACCTGCAGCTGAAG+CGG	0.565189	8.4:-33567234	MS.gene97943:CDS
AAGAAAATATTATGCAGTGC+CGG	0.569979	8.4:-33566409	MS.gene97943:CDS
CTGAACCAGAAGGATGTAGC+AGG	0.571796	8.4:-33566714	MS.gene97943:CDS
GATAGGTGGCAGTCGCAACA+AGG	0.584419	8.4:+33566233	None:intergenic
AGAAACAACAACCATGGCTG+TGG	0.585849	8.4:-33565771	MS.gene97943:CDS
ATTTGGCTTGCATAATGAAG+AGG	0.588329	8.4:+33566924	None:intergenic
GACGCCCTAAAAGTACTAGT+TGG	0.588930	8.4:-33566789	MS.gene97943:CDS
TTAACCTGATAAACTCCACC+AGG	0.594570	8.4:+33566193	None:intergenic
GGTACTTTAGTGTCCAGAAG+TGG	0.609035	8.4:-33565844	MS.gene97943:CDS
TTAAATGCGGCGATATGCAA+AGG	0.609641	8.4:+33567128	None:intergenic
CCTTGTCAAAGTTAAAGGGA+AGG	0.610782	8.4:-33566958	MS.gene97943:CDS
GTGTTTAGTCGACATGAACA+AGG	0.613716	8.4:-33566982	MS.gene97943:CDS
AAATAAAATACTGAACCAGA+AGG	0.625252	8.4:-33566724	MS.gene97943:CDS
TGTGTGAGCATCGGTGACTT+CGG	0.631735	8.4:+33566372	None:intergenic
ATGCCAGGTAGTTAGACTGT+TGG	0.633087	8.4:-33566506	MS.gene97943:intron
ATATGCAGGTGTGTGAGCAT+CGG	0.640634	8.4:+33566363	None:intergenic
AACACGGCATTGAAATGTGT+AGG	0.642219	8.4:+33565926	None:intergenic
TAATGGCAAACCAAGTCACT+GGG	0.645311	8.4:+33565978	None:intergenic
CTATTGAATACTTGACGGTG+AGG	0.647961	8.4:-33566854	MS.gene97943:CDS
ATAGGTGGCAGTCGCAACAA+GGG	0.651423	8.4:+33566234	None:intergenic
TTCAAGAAGTGATCAACATG+AGG	0.657499	8.4:-33566667	MS.gene97943:intron
GCAAAAGAAAGAAAAGACAA+TGG	0.660962	8.4:-33567009	MS.gene97943:CDS
AGGGAGATGGCCGTTAAATG+CGG	0.663051	8.4:+33567115	None:intergenic
CACGGCATTGAAATGTGTAG+GGG	0.667888	8.4:+33565928	None:intergenic
ACTTAGTACTGATTGATAGG+TGG	0.680031	8.4:+33566219	None:intergenic
TATAGCAGAAACAACAACCA+TGG	0.681000	8.4:-33565777	MS.gene97943:CDS
TAACCTGATAAACTCCACCA+GGG	0.698278	8.4:+33566194	None:intergenic
AACCTGATAAACTCCACCAG+GGG	0.700058	8.4:+33566195	None:intergenic
TCACTGTCTTGATTATTGCG+GGG	0.701834	8.4:-33565865	MS.gene97943:CDS
ACCTGATAAACTCCACCAGG+GGG	0.706222	8.4:+33566196	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATTGTTCATTGATTATTAAT+CGG	-	chr8.4:33566457-33566476	MS.gene97943:CDS	15.0%
!!	CATAGAATATAGATAACTAA+TGG	+	chr8.4:33567029-33567048	None:intergenic	20.0%
!!	TTTCAAATTCATAACTTCAA+TGG	+	chr8.4:33565902-33565921	None:intergenic	20.0%
!	AAAAAACATCTCAACAAACA+CGG	+	chr8.4:33567080-33567099	None:intergenic	25.0%
!	AAATAAAATACTGAACCAGA+AGG	-	chr8.4:33566263-33566282	MS.gene97943:CDS	25.0%
!	TGAATCTATTGAATACTTGA+CGG	-	chr8.4:33566128-33566147	MS.gene97943:CDS	25.0%
!	TGACAGTTTATCAATAATAG+TGG	+	chr8.4:33566666-33566685	None:intergenic	25.0%
!	TTTGAACTTAGTTTATATGC+AGG	+	chr8.4:33566641-33566660	None:intergenic	25.0%
!!!	ATACTTATTTTAACTCCAAC+TGG	-	chr8.4:33566962-33566981	MS.gene97943:CDS	25.0%
!!!	CTTTTTCAATTACAGTATAG+AGG	+	chr8.4:33565792-33565811	None:intergenic	25.0%
!!!	TGAATACTTTTAAAGCAGAT+TGG	-	chr8.4:33566372-33566391	MS.gene97943:CDS	25.0%
	AACTAAGAAGATCAGTGTTA+AGG	-	chr8.4:33567179-33567198	MS.gene97943:CDS	30.0%
	AAGAAAATATTATGCAGTGC+CGG	-	chr8.4:33566578-33566597	MS.gene97943:intron	30.0%
	AATAATCAAGACAGTGATAC+TGG	+	chr8.4:33567119-33567138	None:intergenic	30.0%
	AGGTCTAAGTATATCAAGAT+TGG	+	chr8.4:33565743-33565762	None:intergenic	30.0%
	CCTTGAGAAAATAAGCTAAA+AGG	+	chr8.4:33566101-33566120	None:intergenic	30.0%
	GCAAAAGAAAGAAAAGACAA+TGG	-	chr8.4:33565978-33565997	MS.gene97943:CDS	30.0%
	GCTTTCAAGCTAAATAAGAA+AGG	-	chr8.4:33565931-33565950	MS.gene97943:CDS	30.0%
	GTCAAGTATTCAATAGATTC+AGG	+	chr8.4:33566129-33566148	None:intergenic	30.0%
	TACTGCAATTGACATCAAAA+AGG	+	chr8.4:33566942-33566961	None:intergenic	30.0%
	TAGGGTAGAAATAGTAGAAT+TGG	+	chr8.4:33566852-33566871	None:intergenic	30.0%
	TATCACTGTCTTGATTATTG+CGG	-	chr8.4:33567120-33567139	MS.gene97943:CDS	30.0%
	TCATAACTTCAATGGCAAAA+GGG	+	chr8.4:33565894-33565913	None:intergenic	30.0%
	TTCATAACTTCAATGGCAAA+AGG	+	chr8.4:33565895-33565914	None:intergenic	30.0%
	TTTGAAGACAAAGAACTTTG+AGG	-	chr8.4:33566520-33566539	MS.gene97943:intron	30.0%
!	AAAAAGTCCCATCTATTTTG+AGG	-	chr8.4:33565806-33565825	MS.gene97943:CDS	30.0%
!	TCTGTGAATCCATATTTTCT+AGG	+	chr8.4:33567249-33567268	None:intergenic	30.0%
!	TGTCAGAAGTTGATTTCTTT+CGG	-	chr8.4:33566894-33566913	MS.gene97943:CDS	30.0%
!	TGTCTTTTCTTTCTTTTGCA+TGG	+	chr8.4:33565977-33565996	None:intergenic	30.0%
!!	AAAAGTCCCATCTATTTTGA+GGG	-	chr8.4:33565807-33565826	MS.gene97943:CDS	30.0%
!!	AATTCTACTATTTCTACCCT+AGG	-	chr8.4:33566851-33566870	MS.gene97943:CDS	30.0%
!!	ATTTCTTTCGGGTTTCATAT+TGG	-	chr8.4:33566906-33566925	MS.gene97943:CDS	30.0%
!!	TTTAGCTTGAAAGCAAATGA+AGG	+	chr8.4:33565925-33565944	None:intergenic	30.0%
!!!	CCTTTTAGCTTATTTTCTCA+AGG	-	chr8.4:33566098-33566117	MS.gene97943:CDS	30.0%
	AAAGGTCAATCTCTCCAATT+TGG	+	chr8.4:33566083-33566102	None:intergenic	35.0%
	AAGGACCTTGTCAAAGTTAA+AGG	-	chr8.4:33566024-33566043	MS.gene97943:CDS	35.0%
	AGCATTAGACATAGCAACTA+CGG	-	chr8.4:33566550-33566569	MS.gene97943:intron	35.0%
	AGGACCTTGTCAAAGTTAAA+GGG	-	chr8.4:33566025-33566044	MS.gene97943:CDS	35.0%
	ATCACTGTCTTGATTATTGC+GGG	-	chr8.4:33567121-33567140	MS.gene97943:CDS	35.0%
	CTATCTCCCTCAAAATAGAT+GGG	+	chr8.4:33565816-33565835	None:intergenic	35.0%
	CTTCATTATGCAAGCCAAAT+TGG	-	chr8.4:33566066-33566085	MS.gene97943:CDS	35.0%
	GCGCATTACTAATAGCAATA+TGG	+	chr8.4:33566173-33566192	None:intergenic	35.0%
	GGTAAAGGACCTAGAAAATA+TGG	-	chr8.4:33567237-33567256	MS.gene97943:CDS	35.0%
	GGTAGAAATAGTAGAATTGG+AGG	+	chr8.4:33566849-33566868	None:intergenic	35.0%
	TATAGCAGAAACAACAACCA+TGG	-	chr8.4:33567210-33567229	MS.gene97943:CDS	35.0%
	TCTATCTCCCTCAAAATAGA+TGG	+	chr8.4:33565817-33565836	None:intergenic	35.0%
	TGATTATTAATCGGTATGCC+AGG	-	chr8.4:33566466-33566485	MS.gene97943:CDS	35.0%
	TTAACTCCAACTGGAAAAGA+GGG	-	chr8.4:33566971-33566990	MS.gene97943:CDS	35.0%
	TTCAAGAAGTGATCAACATG+AGG	-	chr8.4:33566320-33566339	MS.gene97943:CDS	35.0%
	TTTAACTCCAACTGGAAAAG+AGG	-	chr8.4:33566970-33566989	MS.gene97943:CDS	35.0%
!	GTCAGAAGTTGATTTCTTTC+GGG	-	chr8.4:33566895-33566914	MS.gene97943:CDS	35.0%
!	TTTCTTTCTTTTGCATGGCA+AGG	+	chr8.4:33565972-33565991	None:intergenic	35.0%
!!	ACTTAGTACTGATTGATAGG+TGG	+	chr8.4:33566771-33566790	None:intergenic	35.0%
!!	ATTTGGCTTGCATAATGAAG+AGG	+	chr8.4:33566066-33566085	None:intergenic	35.0%
!!	TCTTAGCTTTTATCCACTTC+TGG	+	chr8.4:33567159-33567178	None:intergenic	35.0%
	ATACCAACAGTCTAACTACC+TGG	+	chr8.4:33566487-33566506	None:intergenic	40.0%
	ATTTCTACCCTAGGAAATGC+TGG	-	chr8.4:33566860-33566879	MS.gene97943:CDS	40.0%
	CCTAGGAAATGCTGGAAAAT+CGG	-	chr8.4:33566868-33566887	MS.gene97943:CDS	40.0%
	CCTTGTCAAAGTTAAAGGGA+AGG	-	chr8.4:33566029-33566048	MS.gene97943:CDS	40.0%
	CTATTGAATACTTGACGGTG+AGG	-	chr8.4:33566133-33566152	MS.gene97943:CDS	40.0%
	CTGGAAAAAGATTACAGGAC+AGG	-	chr8.4:33566225-33566244	MS.gene97943:CDS	40.0%
	GAAGATCAGTGTTAAGGCAT+CGG	-	chr8.4:33567185-33567204	MS.gene97943:CDS	40.0%
	GTCAAAGTTAAAGGGAAGGA+AGG	-	chr8.4:33566033-33566052	MS.gene97943:CDS	40.0%
	GTGTTTAGTCGACATGAACA+AGG	-	chr8.4:33566005-33566024	MS.gene97943:CDS	40.0%
	TAACCTGATAAACTCCACCA+GGG	+	chr8.4:33566796-33566815	None:intergenic	40.0%
	TAATGGCAAACCAAGTCACT+GGG	+	chr8.4:33567012-33567031	None:intergenic	40.0%
	TCACTGTCTTGATTATTGCG+GGG	-	chr8.4:33567122-33567141	MS.gene97943:CDS	40.0%
	TTAAATGCGGCGATATGCAA+AGG	+	chr8.4:33565862-33565881	None:intergenic	40.0%
	TTAACCTGATAAACTCCACC+AGG	+	chr8.4:33566797-33566816	None:intergenic	40.0%
	TTGCATATCGCCGCATTTAA+CGG	-	chr8.4:33565862-33565881	MS.gene97943:CDS	40.0%
!	AACACGGCATTGAAATGTGT+AGG	+	chr8.4:33567064-33567083	None:intergenic	40.0%
!	ACACGGCATTGAAATGTGTA+GGG	+	chr8.4:33567063-33567082	None:intergenic	40.0%
!	CCAGCCAACTAGTACTTTTA+GGG	+	chr8.4:33566205-33566224	None:intergenic	40.0%
!	CCTTCCCTTTAACTTTGACA+AGG	+	chr8.4:33566032-33566051	None:intergenic	40.0%
!	CGATTTTCCAGCATTTCCTA+GGG	+	chr8.4:33566870-33566889	None:intergenic	40.0%
!!	GGGACTTAGTACTGATTGAT+AGG	+	chr8.4:33566774-33566793	None:intergenic	40.0%
	AACAACCATGGCTGTGGTAA+AGG	-	chr8.4:33567222-33567241	MS.gene97943:CDS	45.0%
	AACCTGATAAACTCCACCAG+GGG	+	chr8.4:33566795-33566814	None:intergenic	45.0%
	AGAAACAACAACCATGGCTG+TGG	-	chr8.4:33567216-33567235	MS.gene97943:CDS	45.0%
	ATATGCAGGTGTGTGAGCAT+CGG	+	chr8.4:33566627-33566646	None:intergenic	45.0%
	ATGCCAGGTAGTTAGACTGT+TGG	-	chr8.4:33566481-33566500	MS.gene97943:CDS	45.0%
	CAAGTCACTGGGATGTACAT+TGG	+	chr8.4:33567001-33567020	None:intergenic	45.0%
	CAATGTACATCCCAGTGACT+TGG	-	chr8.4:33566999-33567018	MS.gene97943:CDS	45.0%
	CTAATGGCAAACCAAGTCAC+TGG	+	chr8.4:33567013-33567032	None:intergenic	45.0%
	CTTCAATGGCAAAAGGGAGA+TGG	+	chr8.4:33565888-33565907	None:intergenic	45.0%
	GCTGGCTGGAAAAAGATTAC+AGG	-	chr8.4:33566220-33566239	MS.gene97943:CDS	45.0%
	GGTACTTTAGTGTCCAGAAG+TGG	-	chr8.4:33567143-33567162	MS.gene97943:CDS	45.0%
	TTTGCATGGCAAGGTGGATA+GGG	+	chr8.4:33565963-33565982	None:intergenic	45.0%
!	CACGGCATTGAAATGTGTAG+GGG	+	chr8.4:33567062-33567081	None:intergenic	45.0%
!	CCGATTTTCCAGCATTTCCT+AGG	+	chr8.4:33566871-33566890	None:intergenic	45.0%
!	CTTTCTTTTGCATGGCAAGG+TGG	+	chr8.4:33565969-33565988	None:intergenic	45.0%
!	GCCAGCCAACTAGTACTTTT+AGG	+	chr8.4:33566206-33566225	None:intergenic	45.0%
!	TTTTGCATGGCAAGGTGGAT+AGG	+	chr8.4:33565964-33565983	None:intergenic	45.0%
!!	AAAAGTACTAGTTGGCTGGC+TGG	-	chr8.4:33566206-33566225	MS.gene97943:CDS	45.0%
!!	CCCTAAAAGTACTAGTTGGC+TGG	-	chr8.4:33566202-33566221	MS.gene97943:CDS	45.0%
!!	GACGCCCTAAAAGTACTAGT+TGG	-	chr8.4:33566198-33566217	MS.gene97943:CDS	45.0%
!!!	TTCGGTGCTAGATTTTGCTC+CGG	+	chr8.4:33566600-33566619	None:intergenic	45.0%
	ACCTGATAAACTCCACCAGG+GGG	+	chr8.4:33566794-33566813	None:intergenic	50.0%
	ACTTAGACCTGCAGCTGAAG+CGG	-	chr8.4:33565753-33565772	MS.gene97943:CDS	50.0%
	AGGGAGATGGCCGTTAAATG+CGG	+	chr8.4:33565875-33565894	None:intergenic	50.0%
	ATAGGTGGCAGTCGCAACAA+GGG	+	chr8.4:33566756-33566775	None:intergenic	50.0%
	ATATTACCCGCTTCAGCTGC+AGG	+	chr8.4:33565763-33565782	None:intergenic	50.0%
	CAATCAGTACTAAGTCCCCC+TGG	-	chr8.4:33566776-33566795	MS.gene97943:CDS	50.0%
	CTGAACCAGAAGGATGTAGC+AGG	-	chr8.4:33566273-33566292	MS.gene97943:CDS	50.0%
	TAGGTCCTTTACCACAGCCA+TGG	+	chr8.4:33567230-33567249	None:intergenic	50.0%
	TGTTGCCTGCTACATCCTTC+TGG	+	chr8.4:33566281-33566300	None:intergenic	50.0%
!!	TGTGTGAGCATCGGTGACTT+CGG	+	chr8.4:33566618-33566637	None:intergenic	50.0%
	CTTAGACCTGCAGCTGAAGC+GGG	-	chr8.4:33565754-33565773	MS.gene97943:CDS	55.0%
	GATAGGTGGCAGTCGCAACA+AGG	+	chr8.4:33566757-33566776	None:intergenic	55.0%
	TCAGTACTAAGTCCCCCTGG+TGG	-	chr8.4:33566779-33566798	MS.gene97943:CDS	55.0%
	TCCCCCTGGTGGAGTTTATC+AGG	-	chr8.4:33566790-33566809	MS.gene97943:CDS	55.0%
!	CAGGACAGGTGCTATAGAGC+TGG	-	chr8.4:33566239-33566258	MS.gene97943:CDS	55.0%
!	GGACTGCCCTCTTTTCCAGT+TGG	+	chr8.4:33566980-33566999	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.4	gene	33565736	33567273	33565736	ID=MS.gene97943
chr8.4	mRNA	33565736	33567273	33565736	ID=MS.gene97943.t1;Parent=MS.gene97943
chr8.4	exon	33566668	33567273	33566668	ID=MS.gene97943.t1.exon1;Parent=MS.gene97943.t1
chr8.4	CDS	33566668	33567273	33566668	ID=cds.MS.gene97943.t1;Parent=MS.gene97943.t1
chr8.4	exon	33565736	33566521	33565736	ID=MS.gene97943.t1.exon2;Parent=MS.gene97943.t1
chr8.4	CDS	33565736	33566521	33565736	ID=cds.MS.gene97943.t1;Parent=MS.gene97943.t1

Gene Sequence

>MS.gene97943

ATGACCAATCTTGATATACTTAGACCTGCAGCTGAAGCGGGTAATATAGAAGACCTCTATACTGTAATTGAAAAAGTCCCATCTATTTTGAGGGAGATAGATTTAGAACAATTTGTTGAAACTCCTTTGCATATCGCCGCATTTAACGGCCATCTCCCTTTTGCCATTGAAGTTATGAATTTGAAACCTTCATTTGCTTTCAAGCTAAATAAGAAAGGATTCAGCCCTATCCACCTTGCCATGCAAAAGAAAGAAAAGACAATGGTGTTGTGTTTAGTCGACATGAACAAGGACCTTGTCAAAGTTAAAGGGAAGGAAGGCTTGACTCCTCTTCATTATGCAAGCCAAATTGGAGAGATTGACCTTTTAGCTTATTTTCTCAAGGCATGTCCTGAATCTATTGAATACTTGACGGTGAGGTGCGAGACTGCACTCCATATTGCTATTAGTAATGCGCAGTTCGACGCCCTAAAAGTACTAGTTGGCTGGCTGGAAAAAGATTACAGGACAGGTGCTATAGAGCTGGAAAATAAAATACTGAACCAGAAGGATGTAGCAGGCAACACTATTTTGCACATTTCAGCTTCAAGAAGTGATCAACATGAGGTAATCGTTATTTTATTGAAAGAATTCAAATGAATACTTTTAAAGCAGATTGGTGATTACTAGTACATTCTTCTGAATATTTTAGTAGCTAACATGAATGATCTCTAACAATTGTATTGTTCATTGATTATTAATCGGTATGCCAGGTAGTTAGACTGTTGGTATATACTAAAATAAATTTGAAGACAAAGAACTTTGAGGACAAAACAGCATTAGACATAGCAACTACGGAGAGCAAGAAAATATTATGCAGTGCCGGAGCAAAATCTAGCACCGAAGTCACCGATGCTCACACACCTGCATATAAACTAAGTTCAAAGACCACTATTATTGATAAACTGTCAATTTATATGCTTCGCATATCACGAAATATAAAAGATGAGCAACGTAACACTATGTTGATAGTTGCAGCCCTTGTTGCGACTGCCACCTATCAATCAGTACTAAGTCCCCCTGGTGGAGTTTATCAGGTTAATGCTAGTGACAAAAATTTGAACATCACTTCCTCCAATTCTACTATTTCTACCCTAGGAAATGCTGGAAAATCGGTCATGTCAGAAGTTGATTTCTTTCGGGTTTCATATTGGAATATAGTTTCCTTTTTGATGTCAATTGCAGTAATACTTATTTTAACTCCAACTGGAAAAGAGGGCAGTCCAATGTACATCCCAGTGACTTGGTTTGCCATTAGTTATCTATATTCTATGCGAGTGATATCCCCTACACATTTCAATGCCGTGTTTGTTGAGATGTTTTTTAATTTAATTACGTTTTCCAGTATCACTGTCTTGATTATTGCGGGGTACTTTAGTGTCCAGAAGTGGATAAAAGCTAAGAAACTAAGAAGATCAGTGTTAAGGCATCGGAATATAGCAGAAACAACAACCATGGCTGTGGTAAAGGACCTAGAAAATATGGATTCACAGATTTAA

Protein sequence

>MS.gene97943.t1

MTNLDILRPAAEAGNIEDLYTVIEKVPSILREIDLEQFVETPLHIAAFNGHLPFAIEVMNLKPSFAFKLNKKGFSPIHLAMQKKEKTMVLCLVDMNKDLVKVKGKEGLTPLHYASQIGEIDLLAYFLKACPESIEYLTVRCETALHIAISNAQFDALKVLVGWLEKDYRTGAIELENKILNQKDVAGNTILHISASRSDQHEVVRLLVYTKINLKTKNFEDKTALDIATTESKKILCSAGAKSSTEVTDAHTPAYKLSSKTTIIDKLSIYMLRISRNIKDEQRNTMLIVAALVATATYQSVLSPPGGVYQVNASDKNLNITSSNSTISTLGNAGKSVMSEVDFFRVSYWNIVSFLMSIAVILILTPTGKEGSPMYIPVTWFAISYLYSMRVISPTHFNAVFVEMFFNLITFSSITVLIIAGYFSVQKWIKAKKLRRSVLRHRNIAETTTMAVVKDLENMDSQI