AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene48655

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene48655.t1 MTR_8g072510 68.599 414 85 5 4 374 2 413 0.0 538
MS.gene48655.t1 MTR_8g062410 64.270 459 98 5 4 403 2 453 0.0 536
MS.gene48655.t1 MTR_8g039620 60.145 414 116 5 1 366 1 413 2.34e-165 471
MS.gene48655.t1 MTR_7g021090 61.558 398 97 6 1 352 1 388 9.77e-162 461
MS.gene48655.t1 MTR_3g016210 57.207 444 119 5 4 376 2 445 1.87e-160 459
MS.gene48655.t1 MTR_1g059930 63.712 361 114 2 1 345 1 360 9.55e-160 456
MS.gene48655.t1 MTR_7g451420 68.831 308 95 1 9 316 6 312 1.12e-146 419
MS.gene48655.t1 MTR_8g068990 60.270 370 110 6 1 350 1 353 4.34e-146 422
MS.gene48655.t1 MTR_8g073260 53.538 424 143 5 4 374 2 424 4.68e-144 417
MS.gene48655.t1 MTR_8g069880 53.810 420 142 9 12 381 7 424 2.26e-133 390
MS.gene48655.t1 MTR_8g040930 55.710 359 107 6 54 364 1 355 2.00e-128 375
MS.gene48655.t1 MTR_8g041240 55.710 359 107 6 54 364 1 355 2.00e-128 375
MS.gene48655.t1 MTR_8g467780 50.345 435 151 6 10 381 9 441 2.38e-125 370
MS.gene48655.t1 MTR_8g066670 53.350 403 145 3 9 369 1072 1473 8.97e-125 394
MS.gene48655.t1 MTR_3g105650 45.692 383 178 6 10 365 3 382 3.16e-103 312
MS.gene48655.t1 MTR_8g073275 40.000 420 134 7 54 365 1 410 1.26e-82 260
MS.gene48655.t1 MTR_1g069110 26.409 337 203 8 15 339 21 324 2.19e-23 102
MS.gene48655.t1 MTR_7g099580 24.749 299 171 6 24 316 24 274 3.62e-22 97.1
MS.gene48655.t1 MTR_2g101320 28.111 217 139 3 43 252 137 343 4.64e-19 89.7
MS.gene48655.t1 MTR_7g099570 26.728 217 145 3 6 217 3 210 1.66e-18 87.8
MS.gene48655.t1 MTR_2g101310 26.667 255 159 6 39 276 133 376 5.52e-15 77.0
MS.gene48655.t1 MTR_2g101310 26.667 255 159 6 39 276 133 376 1.03e-14 76.3
MS.gene48655.t1 MTR_7g117620 25.087 287 182 7 47 312 119 393 1.93e-14 75.5
MS.gene48655.t1 MTR_1g115575 24.398 332 198 9 47 352 169 473 3.50e-14 74.7
MS.gene48655.t1 MTR_1g032100 24.615 325 200 8 48 343 95 403 2.58e-13 72.0
MS.gene48655.t1 MTR_8g105260 23.864 352 224 9 47 369 101 437 2.75e-13 71.6
MS.gene48655.t1 MTR_2g096190 25.466 161 118 1 7 167 100 258 1.67e-12 69.3
MS.gene48655.t1 MTR_2g096190 24.074 324 210 8 12 312 70 380 4.15e-12 68.2
MS.gene48655.t1 MTR_4g062100 24.551 167 124 1 7 173 104 268 1.87e-12 69.3
MS.gene48655.t1 MTR_4g062100 23.171 328 208 6 12 312 74 384 3.33e-12 68.6
MS.gene48655.t1 MTR_5g085830 23.810 252 170 5 12 251 247 488 1.69e-11 66.6
MS.gene48655.t1 MTR_2g033050 27.027 222 115 9 17 235 207 384 2.97e-11 65.1
MS.gene48655.t1 MTR_7g100390 26.814 317 179 13 45 312 50 362 5.67e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene48655.t1 AT4G10720 40.390 359 203 3 11 361 4 359 1.53e-84 264
MS.gene48655.t1 AT4G10720 40.525 343 190 3 11 346 4 339 2.94e-80 254
MS.gene48655.t1 AT1G14480 37.709 358 208 3 11 356 4 358 8.99e-77 244
MS.gene48655.t1 AT5G15500 37.531 397 216 6 12 381 6 397 3.39e-76 244
MS.gene48655.t1 AT1G14500 35.328 351 215 2 11 353 4 350 2.45e-71 230
MS.gene48655.t1 AT5G54620 40.333 300 173 2 17 310 10 309 5.64e-69 224
MS.gene48655.t1 AT5G54610 37.500 304 182 2 10 307 3 304 1.48e-68 223
MS.gene48655.t1 AT1G14480 34.682 346 192 5 11 356 4 315 2.68e-55 187
MS.gene48655.t1 AT5G15500 35.433 254 146 5 113 353 1 249 1.18e-37 139
MS.gene48655.t1 AT5G51160 28.364 275 170 5 65 319 34 301 4.74e-26 109
MS.gene48655.t1 AT4G03500 31.308 214 132 4 101 313 280 479 3.64e-20 93.2
MS.gene48655.t1 AT4G03500 31.308 214 132 4 101 313 280 479 3.64e-20 93.2
MS.gene48655.t1 AT4G03500 25.397 378 191 11 12 313 140 502 3.79e-20 93.2
MS.gene48655.t1 AT4G03460 31.696 224 113 6 103 313 258 454 3.38e-18 87.0
MS.gene48655.t1 AT4G03460 31.696 224 113 6 103 313 290 486 3.51e-18 87.0
MS.gene48655.t1 AT1G34050 26.336 262 168 6 68 316 174 423 2.21e-17 84.3
MS.gene48655.t1 AT4G03490 28.065 310 162 10 12 313 211 467 3.93e-16 80.9
MS.gene48655.t1 AT4G03490 28.065 310 162 10 12 313 201 457 5.78e-16 80.1
MS.gene48655.t1 AT4G03490 28.065 310 162 10 12 313 211 467 6.29e-16 80.1
MS.gene48655.t1 AT1G10340 28.814 236 129 5 112 316 205 432 2.00e-15 78.6
MS.gene48655.t1 AT1G10340 28.814 236 129 5 112 316 201 428 2.31e-15 78.2
MS.gene48655.t1 AT4G03480 23.782 349 185 14 12 346 357 638 3.12e-15 78.2
MS.gene48655.t1 AT4G03480 23.782 349 185 14 12 346 360 641 3.15e-15 78.2
MS.gene48655.t1 AT5G54700 29.043 303 143 12 27 328 20 251 4.22e-15 77.4
MS.gene48655.t1 AT5G54710 26.749 243 144 7 98 316 264 496 4.81e-14 74.3
MS.gene48655.t1 AT5G54710 26.749 243 144 7 98 316 219 451 5.05e-14 74.3
MS.gene48655.t1 AT2G01680 22.350 349 233 6 47 369 97 433 1.31e-13 72.8
MS.gene48655.t1 AT3G12360 24.020 408 231 12 11 352 77 471 9.97e-13 70.1
MS.gene48655.t1 AT3G12360 24.020 408 231 12 11 352 65 459 1.05e-12 70.1
MS.gene48655.t1 AT1G07710 24.316 329 177 10 7 312 100 379 1.07e-12 70.1
MS.gene48655.t1 AT1G07710 24.316 329 177 10 7 312 100 379 1.07e-12 70.1
MS.gene48655.t1 AT5G60070 25.455 165 120 2 3 166 103 265 2.82e-12 68.6
MS.gene48655.t1 AT1G03670 27.500 240 136 7 91 319 266 478 1.15e-11 67.0
MS.gene48655.t1 AT5G02620 22.312 372 237 8 12 354 60 408 1.63e-11 66.2
MS.gene48655.t1 AT5G02620 22.312 372 237 8 12 354 60 408 1.63e-11 66.2
MS.gene48655.t1 AT5G02620 22.312 372 237 8 12 354 60 408 1.63e-11 66.2
MS.gene48655.t1 AT1G05640 24.306 288 183 9 47 312 191 465 7.76e-11 64.3
MS.gene48655.t1 AT2G31820 27.072 181 120 3 3 180 253 424 7.92e-11 64.3

Find 75 sgRNAs with CRISPR-Local

Find 109 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACTGGTTAAAACAATTTA+TGG 0.118348 4.3:+67743430 None:intergenic
TTTACATATTGCTGCAACTA+TGG 0.135596 4.3:-67743303 MS.gene48655:CDS
TCAAGATGATCCATCCATTT+TGG 0.183666 4.3:-67743360 MS.gene48655:CDS
AGATTTGAAGTTAAAGAATT+TGG 0.216202 4.3:-67742546 MS.gene48655:CDS
CTTCACTTTGCAAGTCAAAT+TGG 0.229112 4.3:-67743098 MS.gene48655:CDS
AGCACAGGCACTTCTATTAT+TGG 0.259513 4.3:-67742585 MS.gene48655:intron
AAGATTATATTAATAAGTTT+TGG 0.269732 4.3:-67742482 MS.gene48655:CDS
TAGTGGCTTCTCCAATGTTT+TGG 0.292968 4.3:-67742100 MS.gene48655:CDS
TATATCTATATTCTCCAAAA+TGG 0.315194 4.3:+67743346 None:intergenic
AAAGACCTTGTTAGAGTTCA+AGG 0.330305 4.3:-67743140 MS.gene48655:CDS
AGAAACATCAATAAAGCTTA+TGG 0.354374 4.3:-67741887 MS.gene48655:CDS
TCATAATCTCAGAGGCAAAC+TGG 0.386434 4.3:+67743273 None:intergenic
GTCTTGTCAGGATATGAATT+CGG 0.400138 4.3:-67742215 MS.gene48655:intron
TAGTGCTGAAATATGCAAAA+TGG 0.401032 4.3:+67742863 None:intergenic
GAGGCCCTTCAAGTACTTGT+TGG 0.407619 4.3:-67742966 MS.gene48655:CDS
AGGCCCTTCAAGTACTTGTT+GGG 0.409985 4.3:-67742965 MS.gene48655:CDS
CTCCGGTCAAAGACAACAAT+TGG 0.410458 4.3:-67742410 MS.gene48655:CDS
GTCAAGTATTCAATGGATTC+CGG 0.412377 4.3:+67743038 None:intergenic
ACAGAAAGGGGCTCAGGATC+TGG 0.416039 4.3:-67742925 MS.gene48655:CDS
TCTTTAACTTCAAATCTAAA+CGG 0.434446 4.3:+67742552 None:intergenic
ATCCAATTGTTGTCTTTGAC+CGG 0.439154 4.3:+67742408 None:intergenic
CTTGAGCTGCAACATTCAAC+TGG 0.447265 4.3:+67743414 None:intergenic
ATTAATATAATCTTGATCTC+TGG 0.448509 4.3:+67742491 None:intergenic
TGTAAAGGAGTTTCAACAAA+TGG 0.448723 4.3:+67743320 None:intergenic
TCATGGAACTGACATGTGTA+GGG 0.460318 4.3:+67742039 None:intergenic
CTTCTATTATTGGTAAGTAC+TGG 0.476448 4.3:-67742575 MS.gene48655:CDS
GCTGGGAAATCAGTCTTGTC+AGG 0.477588 4.3:-67742227 MS.gene48655:CDS
TCAAAAGAAATGCACAGAAA+GGG 0.477992 4.3:-67742938 MS.gene48655:CDS
TGATCTCTGGAGAGCTTGCT+AGG 0.483666 4.3:+67742504 None:intergenic
CTTGTTAGAGTTCAAGGAAG+AGG 0.493464 4.3:-67743134 MS.gene48655:CDS
GCAACTGCGATCTATCAATC+AGG 0.505224 4.3:-67742299 MS.gene48655:CDS
CTCAAAAGAAATGCACAGAA+AGG 0.511597 4.3:-67742939 MS.gene48655:CDS
AAATGCACAGAAAGGGGCTC+AGG 0.513769 4.3:-67742931 MS.gene48655:CDS
TTACATATTGCTGCAACTAT+GGG 0.515504 4.3:-67743302 MS.gene48655:CDS
TGTTAAGAATGGACAGTATG+AGG 0.523009 4.3:-67742985 MS.gene48655:CDS
AGCACTAAATAGTGACCTGC+AGG 0.525438 4.3:-67742847 MS.gene48655:intron
TCTCACAGTCAAGTATTCAA+TGG 0.527118 4.3:+67743031 None:intergenic
CTACATTTGCACATATTCTC+CGG 0.528807 4.3:-67742427 MS.gene48655:CDS
CCCTTCAAGTACTTGTTGGG+TGG 0.529806 4.3:-67742962 MS.gene48655:CDS
GCCACCCAACAAGTACTTGA+AGG 0.535655 4.3:+67742961 None:intergenic
TCTATATTCTCCAAAATGGA+TGG 0.540983 4.3:+67743350 None:intergenic
GCTTATGGTCATATAGATGA+TGG 0.541409 4.3:-67741872 MS.gene48655:CDS
ATTTGACTTGCAAAGTGAAG+AGG 0.547305 4.3:+67743101 None:intergenic
TTGAATGTTGCAGCTCAAGC+AGG 0.548310 4.3:-67743410 MS.gene48655:CDS
CTGCATGTTGCTGTTAAGAA+TGG 0.551550 4.3:-67742996 MS.gene48655:CDS
ATAGACGATGGACAAAATCA+TGG 0.552060 4.3:+67742022 None:intergenic
ATAGTTGCAGCAATATGTAA+AGG 0.555251 4.3:+67743305 None:intergenic
ATCATGGAACTGACATGTGT+AGG 0.555741 4.3:+67742038 None:intergenic
GCTGCAACTCAAACAACTGC+TGG 0.558587 4.3:-67741839 MS.gene48655:CDS
CGGAGAATATGTGCAAATGT+AGG 0.559700 4.3:+67742428 None:intergenic
TATTGAAACCTTCGTTTGCG+TGG 0.561540 4.3:-67743250 MS.gene48655:CDS
CAGAAGTGCAACGTAACACT+TGG 0.562021 4.3:-67742343 MS.gene48655:CDS
GTTAGAGTTCAAGGAAGAGG+AGG 0.564832 4.3:-67743131 MS.gene48655:CDS
AGTGCTAGTGATGAAAATGC+TGG 0.576236 4.3:-67742245 MS.gene48655:CDS
GCATCGGTGACTTCTAAGCT+AGG 0.583244 4.3:+67742452 None:intergenic
CTAACCTGATATATTCCACC+AGG 0.583479 4.3:+67742266 None:intergenic
TGGTCATATAGATGATGGTA+CGG 0.588819 4.3:-67741867 MS.gene48655:CDS
CAAAAGAAATGCACAGAAAG+GGG 0.590009 4.3:-67742937 MS.gene48655:CDS
ATGTGCAAATGTAGGAGCAT+CGG 0.592512 4.3:+67742436 None:intergenic
CATCTTGAATTACTGTGTAG+AGG 0.592515 4.3:+67743375 None:intergenic
CTCTTCCTTGAACTCTAACA+AGG 0.596189 4.3:+67743135 None:intergenic
CTAGCCACAAACCAAAACAT+TGG 0.596259 4.3:+67742089 None:intergenic
CCACCCAACAAGTACTTGAA+GGG 0.597674 4.3:+67742962 None:intergenic
TTTAGCTTCCACGCAAACGA+AGG 0.618675 4.3:+67743242 None:intergenic
GTGCTAGTGATGAAAATGCT+GGG 0.626440 4.3:-67742244 MS.gene48655:CDS
TGGAACAATAGGACTTCTAG+TGG 0.634853 4.3:-67742117 MS.gene48655:intron
CAGAACTGAACCATAGACGA+TGG 0.647697 4.3:+67742010 None:intergenic
AGAGCTTGCTAGGTCTAATG+CGG 0.657680 4.3:+67742514 None:intergenic
TTTCAATATCATAATCTCAG+AGG 0.659451 4.3:+67743265 None:intergenic
CATGGAACTGACATGTGTAG+GGG 0.679813 4.3:+67742040 None:intergenic
GCGTGGAAGCTAAATCCACA+AGG 0.685594 4.3:-67743233 MS.gene48655:CDS
TAACCTGATATATTCCACCA+GGG 0.695982 4.3:+67742267 None:intergenic
TGGATGGGACTGAATCCTTG+TGG 0.698413 4.3:+67743218 None:intergenic
AACCTGATATATTCCACCAG+GGG 0.698635 4.3:+67742268 None:intergenic
ACCTGATATATTCCACCAGG+GGG 0.735156 4.3:+67742269 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGATTATATTAATAAGTTT+TGG - chr4.3:67742781-67742800 MS.gene48655:intron 10.0%
!! AGACAACTTATAAATAAGAT+AGG - chr4.3:67742592-67742611 MS.gene48655:intron 20.0%
!! ATTAATATAATCTTGATCTC+TGG + chr4.3:67742775-67742794 None:intergenic 20.0%
!! TATATCTATATTCTCCAAAA+TGG + chr4.3:67741920-67741939 None:intergenic 20.0%
!!! AATGTGTTCTTTTTTAGTAA+TGG + chr4.3:67742452-67742471 None:intergenic 20.0%
!!! AGATTTGAAGTTAAAGAATT+TGG - chr4.3:67742717-67742736 MS.gene48655:intron 20.0%
!!! CTAGTTATTTATTTTCTATG+TGG - chr4.3:67743193-67743212 MS.gene48655:CDS 20.0%
!!! TCATATTTCTTGTTACTTTT+TGG - chr4.3:67742528-67742547 MS.gene48655:CDS 20.0%
!!! TCTTTAACTTCAAATCTAAA+CGG + chr4.3:67742714-67742733 None:intergenic 20.0%
! AGAAACATCAATAAAGCTTA+TGG - chr4.3:67743376-67743395 MS.gene48655:CDS 25.0%
! CAACTGGTTAAAACAATTTA+TGG + chr4.3:67741836-67741855 None:intergenic 25.0%
! TGCTAAAATTGATACAGAAA+AGG + chr4.3:67743100-67743119 None:intergenic 25.0%
! TTTCAATATCATAATCTCAG+AGG + chr4.3:67742001-67742020 None:intergenic 25.0%
!! AGAATGAAATTTTAAACAGG+AGG - chr4.3:67742361-67742380 MS.gene48655:CDS 25.0%
!! GAATGAAATTTTAAACAGGA+GGG - chr4.3:67742362-67742381 MS.gene48655:CDS 25.0%
!! TGGAGAATGAAATTTTAAAC+AGG - chr4.3:67742358-67742377 MS.gene48655:CDS 25.0%
!! TGTATCGTTCTTTAATTTCT+TGG - chr4.3:67742654-67742673 MS.gene48655:intron 25.0%
!!! AGTTAAAGCATTGTTGAAAT+AGG - chr4.3:67743353-67743372 MS.gene48655:CDS 25.0%
!!! GTTATTTATTTTCTATGTGG+AGG - chr4.3:67743196-67743215 MS.gene48655:CDS 25.0%
ATAGTTGCAGCAATATGTAA+AGG + chr4.3:67741961-67741980 None:intergenic 30.0%
CTTCTATTATTGGTAAGTAC+TGG - chr4.3:67742688-67742707 MS.gene48655:intron 30.0%
GCAAAACGACAAAAAACATA+TGG - chr4.3:67742077-67742096 MS.gene48655:CDS 30.0%
TCAAAAGAAATGCACAGAAA+GGG - chr4.3:67742325-67742344 MS.gene48655:CDS 30.0%
TCTATATTCTCCAAAATGGA+TGG + chr4.3:67741916-67741935 None:intergenic 30.0%
TGTAAAGGAGTTTCAACAAA+TGG + chr4.3:67741946-67741965 None:intergenic 30.0%
TTACATATTGCTGCAACTAT+GGG - chr4.3:67741961-67741980 MS.gene48655:intron 30.0%
TTTACATATTGCTGCAACTA+TGG - chr4.3:67741960-67741979 MS.gene48655:intron 30.0%
! GTTGTCTTTCATGTTTTTCA+AGG + chr4.3:67742579-67742598 None:intergenic 30.0%
! TAGTGCTGAAATATGCAAAA+TGG + chr4.3:67742403-67742422 None:intergenic 30.0%
!! CATAACAGTCATTTTCTTTC+AGG + chr4.3:67742480-67742499 None:intergenic 30.0%
AAAGACCTTGTTAGAGTTCA+AGG - chr4.3:67742123-67742142 MS.gene48655:intron 35.0%
ATAGACGATGGACAAAATCA+TGG + chr4.3:67743244-67743263 None:intergenic 35.0%
ATCCAATTGTTGTCTTTGAC+CGG + chr4.3:67742858-67742877 None:intergenic 35.0%
CAAAAGAAATGCACAGAAAG+GGG - chr4.3:67742326-67742345 MS.gene48655:CDS 35.0%
CAATAATGACATCAGTAACC+AGG + chr4.3:67743284-67743303 None:intergenic 35.0%
CATCTTGAATTACTGTGTAG+AGG + chr4.3:67741891-67741910 None:intergenic 35.0%
CTACATTTGCACATATTCTC+CGG - chr4.3:67742836-67742855 MS.gene48655:intron 35.0%
CTCAAAAGAAATGCACAGAA+AGG - chr4.3:67742324-67742343 MS.gene48655:CDS 35.0%
CTGGTTACTGATGTCATTAT+TGG - chr4.3:67743282-67743301 MS.gene48655:CDS 35.0%
CTTCACTTTGCAAGTCAAAT+TGG - chr4.3:67742165-67742184 MS.gene48655:intron 35.0%
GCTTATGGTCATATAGATGA+TGG - chr4.3:67743391-67743410 MS.gene48655:CDS 35.0%
GTCTTGTCAGGATATGAATT+CGG - chr4.3:67743048-67743067 MS.gene48655:CDS 35.0%
TAACCTGATATATTCCACCA+GGG + chr4.3:67742999-67743018 None:intergenic 35.0%
TCTCACAGTCAAGTATTCAA+TGG + chr4.3:67742235-67742254 None:intergenic 35.0%
TGTTAAGAATGGACAGTATG+AGG - chr4.3:67742278-67742297 MS.gene48655:CDS 35.0%
! AATTTTAAACAGGAGGGATG+AGG - chr4.3:67742368-67742387 MS.gene48655:CDS 35.0%
! ATGATTTTGTCCATCGTCTA+TGG - chr4.3:67743243-67743262 MS.gene48655:CDS 35.0%
! CTTTAATTTCTTGGTAGCAC+AGG - chr4.3:67742663-67742682 MS.gene48655:intron 35.0%
! GTCAAGTATTCAATGGATTC+CGG + chr4.3:67742228-67742247 None:intergenic 35.0%
! TCAAGATGATCCATCCATTT+TGG - chr4.3:67741903-67741922 MS.gene48655:intron 35.0%
! TGACTTTCTCTTTTTGTGTC+CGG - chr4.3:67742206-67742225 MS.gene48655:intron 35.0%
! TGGTCATATAGATGATGGTA+CGG - chr4.3:67743396-67743415 MS.gene48655:CDS 35.0%
!! ATTTGACTTGCAAAGTGAAG+AGG + chr4.3:67742165-67742184 None:intergenic 35.0%
!!! TTCTCCAATGTTTTGGTTTG+TGG - chr4.3:67743170-67743189 MS.gene48655:CDS 35.0%
AACCTGATATATTCCACCAG+GGG + chr4.3:67742998-67743017 None:intergenic 40.0%
AATGGCATAAGATTACCTGC+AGG + chr4.3:67742434-67742453 None:intergenic 40.0%
AGCACAGGCACTTCTATTAT+TGG - chr4.3:67742678-67742697 MS.gene48655:intron 40.0%
AGTGCTAGTGATGAAAATGC+TGG - chr4.3:67743018-67743037 MS.gene48655:CDS 40.0%
ATCATGGAACTGACATGTGT+AGG + chr4.3:67743228-67743247 None:intergenic 40.0%
CGGAGAATATGTGCAAATGT+AGG + chr4.3:67742838-67742857 None:intergenic 40.0%
CTAACCTGATATATTCCACC+AGG + chr4.3:67743000-67743019 None:intergenic 40.0%
CTAGCCACAAACCAAAACAT+TGG + chr4.3:67743177-67743196 None:intergenic 40.0%
CTCTTCCTTGAACTCTAACA+AGG + chr4.3:67742131-67742150 None:intergenic 40.0%
CTTGTTAGAGTTCAAGGAAG+AGG - chr4.3:67742129-67742148 MS.gene48655:intron 40.0%
GTGCTAGTGATGAAAATGCT+GGG - chr4.3:67743019-67743038 MS.gene48655:CDS 40.0%
TATTGAAACCTTCGTTTGCG+TGG - chr4.3:67742013-67742032 MS.gene48655:CDS 40.0%
TCATAATCTCAGAGGCAAAC+TGG + chr4.3:67741993-67742012 None:intergenic 40.0%
TCATGGAACTGACATGTGTA+GGG + chr4.3:67743227-67743246 None:intergenic 40.0%
TCATTATTGGAGTGTGCAGA+AGG - chr4.3:67743295-67743314 MS.gene48655:CDS 40.0%
TGGAACAATAGGACTTCTAG+TGG - chr4.3:67743146-67743165 MS.gene48655:CDS 40.0%
TTTATGATACCACTACCACG+TGG - chr4.3:67743126-67743145 MS.gene48655:CDS 40.0%
! ATGTGCAAATGTAGGAGCAT+CGG + chr4.3:67742830-67742849 None:intergenic 40.0%
! CTGCATGTTGCTGTTAAGAA+TGG - chr4.3:67742267-67742286 MS.gene48655:CDS 40.0%
! TAGTGGCTTCTCCAATGTTT+TGG - chr4.3:67743163-67743182 MS.gene48655:CDS 40.0%
! TTTTGCATAGCGAGATGGAT+GGG + chr4.3:67742063-67742082 None:intergenic 40.0%
ACCTGATATATTCCACCAGG+GGG + chr4.3:67742997-67743016 None:intergenic 45.0%
AGAAGTCCTATTGTTCCACG+TGG + chr4.3:67743144-67743163 None:intergenic 45.0%
AGCACTAAATAGTGACCTGC+AGG - chr4.3:67742416-67742435 MS.gene48655:CDS 45.0%
AGGCCCTTCAAGTACTTGTT+GGG - chr4.3:67742298-67742317 MS.gene48655:CDS 45.0%
CAGAACTGAACCATAGACGA+TGG + chr4.3:67743256-67743275 None:intergenic 45.0%
CATGGAACTGACATGTGTAG+GGG + chr4.3:67743226-67743245 None:intergenic 45.0%
CCACCCAACAAGTACTTGAA+GGG + chr4.3:67742304-67742323 None:intergenic 45.0%
CTCCGGTCAAAGACAACAAT+TGG - chr4.3:67742853-67742872 MS.gene48655:CDS 45.0%
CTTGAGCTGCAACATTCAAC+TGG + chr4.3:67741852-67741871 None:intergenic 45.0%
GCAACTGCGATCTATCAATC+AGG - chr4.3:67742964-67742983 MS.gene48655:CDS 45.0%
GTTAGAGTTCAAGGAAGAGG+AGG - chr4.3:67742132-67742151 MS.gene48655:intron 45.0%
TGGTTCAGTTCTGTTAGTCC+TGG - chr4.3:67743263-67743282 MS.gene48655:CDS 45.0%
TTGAATGTTGCAGCTCAAGC+AGG - chr4.3:67741853-67741872 MS.gene48655:CDS 45.0%
! CAGAAGTGCAACGTAACACT+TGG - chr4.3:67742920-67742939 MS.gene48655:CDS 45.0%
! GTTTTGCATAGCGAGATGGA+TGG + chr4.3:67742064-67742083 None:intergenic 45.0%
! TTTAGCTTCCACGCAAACGA+AGG + chr4.3:67742024-67742043 None:intergenic 45.0%
!! AGAGCTTGCTAGGTCTAATG+CGG + chr4.3:67742752-67742771 None:intergenic 45.0%
!!! TGTCGTTTTGCATAGCGAGA+TGG + chr4.3:67742068-67742087 None:intergenic 45.0%
AAATGCACAGAAAGGGGCTC+AGG - chr4.3:67742332-67742351 MS.gene48655:CDS 50.0%
CCACTACCACGTGGAACAAT+AGG - chr4.3:67743135-67743154 MS.gene48655:CDS 50.0%
CCTATTGTTCCACGTGGTAG+TGG + chr4.3:67743138-67743157 None:intergenic 50.0%
GAGGCCCTTCAAGTACTTGT+TGG - chr4.3:67742297-67742316 MS.gene48655:CDS 50.0%
GCCACCCAACAAGTACTTGA+AGG + chr4.3:67742305-67742324 None:intergenic 50.0%
GCGTGGAAGCTAAATCCACA+AGG - chr4.3:67742030-67742049 MS.gene48655:CDS 50.0%
GCTGCAACTCAAACAACTGC+TGG - chr4.3:67743424-67743443 MS.gene48655:CDS 50.0%
TCCCCCTGGTGGAATATATC+AGG - chr4.3:67742993-67743012 MS.gene48655:CDS 50.0%
TGGATGGGACTGAATCCTTG+TGG + chr4.3:67742048-67742067 None:intergenic 50.0%
! CCCTTCAAGTACTTGTTGGG+TGG - chr4.3:67742301-67742320 MS.gene48655:CDS 50.0%
! GCATCGGTGACTTCTAAGCT+AGG + chr4.3:67742814-67742833 None:intergenic 50.0%
! GCTGGGAAATCAGTCTTGTC+AGG - chr4.3:67743036-67743055 MS.gene48655:CDS 50.0%
! TGATCTCTGGAGAGCTTGCT+AGG + chr4.3:67742762-67742781 None:intergenic 50.0%
ACAGAAAGGGGCTCAGGATC+TGG - chr4.3:67742338-67742357 MS.gene48655:CDS 55.0%
CAATCAGGATTGAGTCCCCC+TGG - chr4.3:67742979-67742998 MS.gene48655:CDS 55.0%
TCAGGATTGAGTCCCCCTGG+TGG - chr4.3:67742982-67743001 MS.gene48655:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 67741820 67743465 67741820 ID=MS.gene48655
chr4.3 mRNA 67741820 67743465 67741820 ID=MS.gene48655.t1;Parent=MS.gene48655
chr4.3 exon 67742848 67743465 67742848 ID=MS.gene48655.t1.exon1;Parent=MS.gene48655.t1
chr4.3 CDS 67742848 67743465 67742848 ID=cds.MS.gene48655.t1;Parent=MS.gene48655.t1
chr4.3 exon 67742216 67742600 67742216 ID=MS.gene48655.t1.exon2;Parent=MS.gene48655.t1
chr4.3 CDS 67742216 67742600 67742216 ID=cds.MS.gene48655.t1;Parent=MS.gene48655.t1
chr4.3 exon 67742011 67742128 67742011 ID=MS.gene48655.t1.exon3;Parent=MS.gene48655.t1
chr4.3 CDS 67742011 67742128 67742011 ID=cds.MS.gene48655.t1;Parent=MS.gene48655.t1
chr4.3 exon 67741820 67741910 67741820 ID=MS.gene48655.t1.exon4;Parent=MS.gene48655.t1
chr4.3 CDS 67741820 67741910 67741820 ID=cds.MS.gene48655.t1;Parent=MS.gene48655.t1
Gene Sequence

>MS.gene48655

ATGAACACAAATACCATAAATTGTTTTAACCAGTTGAATGTTGCAGCTCAAGCAGGTGATATAGATCTCCTCTACACAGTAATTCAAGATGATCCATCCATTTTGGAGAATATAGATATAATACCATTTGTTGAAACTCCTTTACATATTGCTGCAACTATGGGTCATCTCCAGTTTGCCTCTGAGATTATGATATTGAAACCTTCGTTTGCGTGGAAGCTAAATCCACAAGGATTCAGTCCCATCCATCTCGCTATGCAAAACGACAAAAAACATATGGTGTATCGCTTTGTGTACATCAATAAAGACCTTGTTAGAGTTCAAGGAAGAGGAGGCTTAACTCCTCTTCACTTTGCAAGTCAAATTGGTGAGATTGAAATTTTAGCTGACTTTCTCTTTTTGTGTCCGGAATCCATTGAATACTTGACTGTGAGACGCGAGACTGCACTGCATGTTGCTGTTAAGAATGGACAGTATGAGGCCCTTCAAGTACTTGTTGGGTGGCTCAAAAGAAATGCACAGAAAGGGGCTCAGGATCTGGAGAATGAAATTTTAAACAGGAGGGATGAGGAACACAACACCATTTTGCATATTTCAGCACTAAATAGTGACCTGCAGGTAATCTTATGCCATTACTAAAAAAGAACACATTAAGAACCTGAAAGAAAATGACTGTTATGTTTCTTTTTATTTTGTTGTGAATGATGATCATATTTCTTGTTACTTTTTGGTCTTCTACTTCTTCGTCATCTTTTTCCTTGAAAAACATGAAAGACAACTTATAAATAAGATAGGTTGCTTAGTTGCTAGCTAACATGAATCATCTCTAACAATTGTATCGTTCTTTAATTTCTTGGTAGCACAGGCACTTCTATTATTGGTAAGTACTGGCCGTTTAGATTTGAAGTTAAAGAATTTGGAAAACAAAACCGCATTAGACCTAGCAAGCTCTCCAGAGATCAAGATTATATTAATAAGTTTTGGAGCAAAACCTAGCTTAGAAGTCACCGATGCTCCTACATTTGCACATATTCTCCGGTCAAAGACAACAATTGGATATAATATGAGAGTTTTTAAAACTCGCATTAATAGAAACATATCAGAAGTGCAACGTAACACTTGGTTGATAGTTGCGACTCTTGTTGCAACTGCGATCTATCAATCAGGATTGAGTCCCCCTGGTGGAATATATCAGGTTAGTGCTAGTGATGAAAATGCTGGGAAATCAGTCTTGTCAGGATATGAATTCGGTTTGTTTTTAATTGTAAATATGTATTCCTTTTCTGTATCAATTTTAGCAATATTTTTTATGATACCACTACCACGTGGAACAATAGGACTTCTAGTGGCTTCTCCAATGTTTTGGTTTGTGGCTAGTTATTTATTTTCTATGTGGAGGATATCCCCTACACATGTCAGTTCCATGATTTTGTCCATCGTCTATGGTTCAGTTCTGTTAGTCCTGGTTACTGATGTCATTATTGGAGTGTGCAGAAGGCTTATACACAGAATTGCTAAGATATTTAATATGAAAGTTAAAGCATTGTTGAAATAGGAGAAACATCAATAAAGCTTATGGTCATATAGATGATGGTACGGAGATCGCTGCAACTCAAACAACTGCTGGTCGAATTCACAACCAATAA
Protein sequence

>MS.gene48655.t1

MNTNTINCFNQLNVAAQAGDIDLLYTVIQDDPSILENIDIIPFVETPLHIAATMGHLQFASEIMILKPSFAWKLNPQGFSPIHLAMQNDKKHMVYRFVYINKDLVRVQGRGGLTPLHFASQIGEIEILADFLFLCPESIEYLTVRRETALHVAVKNGQYEALQVLVGWLKRNAQKGAQDLENEILNRRDEEHNTILHISALNSDLQALLLLVSTGRLDLKLKNLENKTALDLASSPEIKIILISFGAKPSLEVTDAPTFAHILRSKTTIGYNMRVFKTRINRNISEVQRNTWLIVATLVATAIYQSGLSPPGGIYQVSASDENAGKSVLSGYEFGLLVASPMFWFVASYLFSMWRISPTHVSSMILSIVYGSVLRNINKAYGHIDDGTEIAATQTTAGRIHNQ