AlfalfaGEDB | MS.gene78281

M. sativa cultivar XinJiangDaYe / MS.gene78281

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF12796.10 PF13962.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene78281.t1	XP_003598604.2	52.8	356	149	4	3	342	4	356	1.30E-72	283.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene78281.t1	Q8GYH5	50.2	301	131	6	15	304	11	303	1.3e-17	92.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene78281.t1	G7IYT6	52.8	356	149	4	3	342	4	356	9.5e-73	283.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049171	MS.gene78281	0.826979	2.01E-54	-1.69E-46
MS.gene049222	MS.gene78281	0.832602	8.61E-56	-1.69E-46
MS.gene049232	MS.gene78281	0.82905	6.39E-55	-1.69E-46
MS.gene049285	MS.gene78281	0.833196	6.13E-56	-1.69E-46
MS.gene049320	MS.gene78281	0.824907	6.26E-54	-1.69E-46
MS.gene049552	MS.gene78281	0.812135	4.94E-51	-1.69E-46
MS.gene049609	MS.gene78281	0.834317	3.21E-56	-1.69E-46
MS.gene049656	MS.gene78281	0.827683	1.37E-54	-1.69E-46
MS.gene049657	MS.gene78281	0.836503	9.01E-57	-1.69E-46
MS.gene05010	MS.gene78281	0.819541	1.10E-52	-1.69E-46
MS.gene050170	MS.gene78281	0.844892	5.70E-59	-1.69E-46
MS.gene050323	MS.gene78281	0.819136	1.36E-52	-1.69E-46
MS.gene050496	MS.gene78281	0.82791	1.20E-54	-1.69E-46
MS.gene050504	MS.gene78281	0.826202	3.09E-54	-1.69E-46
MS.gene050577	MS.gene78281	0.849478	3.15E-60	-1.69E-46
MS.gene050612	MS.gene78281	0.800653	1.30E-48	-1.69E-46
MS.gene050675	MS.gene78281	0.802254	6.12E-49	-1.69E-46
MS.gene050699	MS.gene78281	0.853847	1.82E-61	-1.69E-46
MS.gene050747	MS.gene78281	0.853249	2.71E-61	-1.69E-46
MS.gene050772	MS.gene78281	0.834804	2.42E-56	-1.69E-46
MS.gene050868	MS.gene78281	0.824067	9.87E-54	-1.69E-46
MS.gene050871	MS.gene78281	0.824964	6.07E-54	-1.69E-46
MS.gene050883	MS.gene78281	-0.801881	7.30E-49	-1.69E-46
MS.gene050950	MS.gene78281	0.844056	9.57E-59	-1.69E-46
MS.gene050986	MS.gene78281	0.815902	7.28E-52	-1.69E-46
MS.gene051551	MS.gene78281	0.832789	7.73E-56	-1.69E-46
MS.gene051560	MS.gene78281	0.801981	6.96E-49	-1.69E-46
MS.gene052164	MS.gene78281	0.82687	2.14E-54	-1.69E-46
MS.gene052482	MS.gene78281	0.837004	6.71E-57	-1.69E-46
MS.gene052486	MS.gene78281	0.850587	1.54E-60	-1.69E-46
MS.gene052501	MS.gene78281	0.813236	2.84E-51	-1.69E-46
MS.gene052503	MS.gene78281	0.871349	7.31E-67	-1.69E-46
MS.gene052506	MS.gene78281	0.814771	1.30E-51	-1.69E-46
MS.gene052615	MS.gene78281	0.848058	7.80E-60	-1.69E-46
MS.gene052823	MS.gene78281	0.872485	3.07E-67	-1.69E-46
MS.gene052825	MS.gene78281	0.866729	2.30E-65	-1.69E-46
MS.gene052966	MS.gene78281	0.811222	7.80E-51	-1.69E-46
MS.gene053203	MS.gene78281	0.852095	5.78E-61	-1.69E-46
MS.gene053213	MS.gene78281	0.860057	2.69E-63	-1.69E-46
MS.gene053286	MS.gene78281	0.858173	9.88E-63	-1.69E-46
MS.gene053436	MS.gene78281	0.880392	5.70E-70	-1.69E-46
MS.gene053480	MS.gene78281	0.821903	3.15E-53	-1.69E-46
MS.gene053549	MS.gene78281	-0.808811	2.58E-50	-1.69E-46
MS.gene053574	MS.gene78281	0.863815	1.90E-64	-1.69E-46
MS.gene053626	MS.gene78281	0.854026	1.62E-61	-1.69E-46
MS.gene053824	MS.gene78281	0.817688	2.90E-52	-1.69E-46
MS.gene054003	MS.gene78281	0.828838	7.20E-55	-1.69E-46
MS.gene054408	MS.gene78281	0.825365	4.88E-54	-1.69E-46
MS.gene054595	MS.gene78281	0.84497	5.44E-59	-1.69E-46
MS.gene054600	MS.gene78281	0.806726	7.15E-50	-1.69E-46
MS.gene054642	MS.gene78281	0.800769	1.23E-48	-1.69E-46
MS.gene05470	MS.gene78281	0.815411	9.37E-52	-1.69E-46
MS.gene054927	MS.gene78281	0.84062	7.79E-58	-1.69E-46
MS.gene054954	MS.gene78281	0.839484	1.54E-57	-1.69E-46
MS.gene055362	MS.gene78281	0.849614	2.89E-60	-1.69E-46
MS.gene055502	MS.gene78281	0.841668	4.13E-58	-1.69E-46
MS.gene055577	MS.gene78281	0.80768	4.49E-50	-1.69E-46
MS.gene055722	MS.gene78281	0.812862	3.42E-51	-1.69E-46
MS.gene05589	MS.gene78281	0.82626	2.99E-54	-1.69E-46
MS.gene056040	MS.gene78281	0.851513	8.45E-61	-1.69E-46
MS.gene056046	MS.gene78281	0.819557	1.09E-52	-1.69E-46
MS.gene056166	MS.gene78281	0.850736	1.40E-60	-1.69E-46
MS.gene056374	MS.gene78281	0.800349	1.50E-48	-1.69E-46
MS.gene056446	MS.gene78281	0.818738	1.68E-52	-1.69E-46
MS.gene056448	MS.gene78281	0.803885	2.82E-49	-1.69E-46
MS.gene056504	MS.gene78281	0.851755	7.22E-61	-1.69E-46
MS.gene056510	MS.gene78281	0.851189	1.04E-60	-1.69E-46
MS.gene056610	MS.gene78281	0.809598	1.75E-50	-1.69E-46
MS.gene056657	MS.gene78281	0.825283	5.10E-54	-1.69E-46
MS.gene056878	MS.gene78281	0.848147	7.38E-60	-1.69E-46
MS.gene05726	MS.gene78281	-0.827137	1.85E-54	-1.69E-46
MS.gene057416	MS.gene78281	0.819119	1.37E-52	-1.69E-46
MS.gene057831	MS.gene78281	0.830404	2.99E-55	-1.69E-46
MS.gene05787	MS.gene78281	0.830256	3.25E-55	-1.69E-46
MS.gene058157	MS.gene78281	0.818555	1.84E-52	-1.69E-46
MS.gene058338	MS.gene78281	0.803556	3.29E-49	-1.69E-46
MS.gene05837	MS.gene78281	0.849796	2.57E-60	-1.69E-46
MS.gene058419	MS.gene78281	0.835484	1.63E-56	-1.69E-46
MS.gene058544	MS.gene78281	0.842264	2.88E-58	-1.69E-46
MS.gene058838	MS.gene78281	0.805015	1.64E-49	-1.69E-46
MS.gene05883	MS.gene78281	0.807241	5.57E-50	-1.69E-46
MS.gene059022	MS.gene78281	0.835116	2.02E-56	-1.69E-46
MS.gene059023	MS.gene78281	0.825971	3.50E-54	-1.69E-46
MS.gene059303	MS.gene78281	0.810165	1.32E-50	-1.69E-46
MS.gene059677	MS.gene78281	0.810497	1.12E-50	-1.69E-46
MS.gene059718	MS.gene78281	0.836076	1.16E-56	-1.69E-46
MS.gene059756	MS.gene78281	0.833804	4.32E-56	-1.69E-46
MS.gene059801	MS.gene78281	0.805156	1.53E-49	-1.69E-46
MS.gene059837	MS.gene78281	0.841386	4.90E-58	-1.69E-46
MS.gene059865	MS.gene78281	0.817828	2.69E-52	-1.69E-46
MS.gene05996	MS.gene78281	0.813929	2.00E-51	-1.69E-46
MS.gene06000	MS.gene78281	0.814898	1.22E-51	-1.69E-46
MS.gene060482	MS.gene78281	0.836348	9.86E-57	-1.69E-46
MS.gene060485	MS.gene78281	0.804927	1.71E-49	-1.69E-46
MS.gene060595	MS.gene78281	-0.81386	2.07E-51	-1.69E-46
MS.gene060778	MS.gene78281	0.848991	4.31E-60	-1.69E-46
MS.gene06077	MS.gene78281	0.875965	2.03E-68	-1.69E-46
MS.gene060982	MS.gene78281	0.836942	6.96E-57	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene78281	MS.gene25024	PPI
MS.gene78281	MS.gene012203	PPI
MS.gene78281	MS.gene26019	PPI
MS.gene78281	MS.gene050272	PPI
MS.gene78281	MS.gene63085	PPI
MS.gene78281	MS.gene69687	PPI
MS.gene78281	MS.gene26532	PPI
MS.gene78281	MS.gene050456	PPI
MS.gene78281	MS.gene30176	PPI
MS.gene47479	MS.gene78281	PPI
MS.gene78281	MS.gene51258	PPI
MS.gene78281	MS.gene32723	PPI
MS.gene78281	MS.gene010910	PPI
MS.gene78281	MS.gene053363	PPI
MS.gene78281	MS.gene066467	PPI
MS.gene78281	MS.gene024753	PPI
MS.gene78281	MS.gene32726	PPI
MS.gene78281	MS.gene71122	PPI
MS.gene78281	MS.gene24922	PPI
MS.gene78281	MS.gene052956	PPI
MS.gene78281	MS.gene73119	PPI
MS.gene78281	MS.gene92243	PPI
MS.gene78281	MS.gene85534	PPI
MS.gene78281	MS.gene037950	PPI
MS.gene78281	MS.gene027074	PPI
MS.gene78281	MS.gene51262	PPI
MS.gene78281	MS.gene23881	PPI
MS.gene78281	MS.gene26672	PPI
MS.gene78281	MS.gene014931	PPI
MS.gene78281	MS.gene21888	PPI
MS.gene056455	MS.gene78281	PPI
MS.gene78281	MS.gene97379	PPI
MS.gene78281	MS.gene066500	PPI
MS.gene78281	MS.gene23852	PPI
MS.gene78281	MS.gene39623	PPI
MS.gene78281	MS.gene36842	PPI
MS.gene78281	MS.gene066464	PPI
MS.gene78281	MS.gene07706	PPI
MS.gene78281	MS.gene071113	PPI
MS.gene78281	MS.gene90400	PPI
MS.gene78281	MS.gene022109	PPI
MS.gene039682	MS.gene78281	PPI
MS.gene78281	MS.gene041770	PPI
MS.gene78281	MS.gene81055	PPI
MS.gene78281	MS.gene65775	PPI
MS.gene78281	MS.gene99554	PPI
MS.gene78281	MS.gene80163	PPI
MS.gene78281	MS.gene02469	PPI
MS.gene78281	MS.gene33377	PPI
MS.gene78281	MS.gene51335	PPI
MS.gene78281	MS.gene045937	PPI
MS.gene78281	MS.gene027072	PPI
MS.gene23852	MS.gene78281	PPI
MS.gene78281	MS.gene51278	PPI
MS.gene78281	MS.gene69966	PPI
MS.gene78281	MS.gene87218	PPI
MS.gene78281	MS.gene025229	PPI
MS.gene78281	MS.gene30433	PPI
MS.gene78281	MS.gene066501	PPI
MS.gene78281	MS.gene043742	PPI
MS.gene78281	MS.gene062662	PPI
MS.gene78281	MS.gene027441	PPI
MS.gene78281	MS.gene21734	PPI
MS.gene78281	MS.gene30177	PPI
MS.gene78281	MS.gene70008	PPI
MS.gene78281	MS.gene008767	PPI
MS.gene78281	MS.gene008833	PPI
MS.gene69687	MS.gene78281	PPI
MS.gene78281	MS.gene23811	PPI
MS.gene78281	MS.gene25244	PPI
MS.gene78281	MS.gene69965	PPI
MS.gene69965	MS.gene78281	PPI
MS.gene78281	MS.gene82740	PPI
MS.gene78281	MS.gene051003	PPI
MS.gene78281	MS.gene48655	PPI
MS.gene78281	MS.gene09785	PPI
MS.gene78281	MS.gene64441	PPI
MS.gene78281	MS.gene066046	PPI
MS.gene78281	MS.gene007486	PPI
MS.gene78281	MS.gene035805	PPI
MS.gene78281	MS.gene23810	PPI
MS.gene78281	MS.gene08470	PPI
MS.gene78281	MS.gene027073	PPI
MS.gene30172	MS.gene78281	PPI
MS.gene78281	MS.gene34712	PPI
MS.gene78281	MS.gene65463	PPI
MS.gene78281	MS.gene007485	PPI
MS.gene78281	MS.gene007487	PPI
MS.gene78281	MS.gene037184	PPI
MS.gene027071	MS.gene78281	PPI
MS.gene78281	MS.gene23196	PPI
MS.gene78281	MS.gene039682	PPI
MS.gene78281	MS.gene067705	PPI
MS.gene78281	MS.gene71204	PPI
MS.gene78281	MS.gene41291	PPI
MS.gene010910	MS.gene78281	PPI
MS.gene78281	MS.gene027075	PPI
MS.gene78281	MS.gene061932	PPI
MS.gene78281	MS.gene050457	PPI
MS.gene063222	MS.gene78281	PPI
MS.gene78281	MS.gene30174	PPI
MS.gene78281	MS.gene26021	PPI
MS.gene78281	MS.gene99973	PPI
MS.gene010912	MS.gene78281	PPI
MS.gene78281	MS.gene064542	PPI
MS.gene78281	MS.gene91775	PPI
MS.gene78281	MS.gene25515	PPI
MS.gene78281	MS.gene34651	PPI
MS.gene78281	MS.gene056455	PPI
MS.gene78281	MS.gene89986	PPI
MS.gene78281	MS.gene22482	PPI
MS.gene78281	MS.gene050270	PPI
MS.gene78281	MS.gene60920	PPI
MS.gene78281	MS.gene058128	PPI
MS.gene78281	MS.gene34009	PPI
MS.gene78281	MS.gene67157	PPI
MS.gene78281	MS.gene018518	PPI
MS.gene78281	MS.gene043662	PPI
MS.gene78281	MS.gene065848	PPI
MS.gene78281	MS.gene004844	PPI
MS.gene78281	MS.gene068249	PPI
MS.gene78281	MS.gene99588	PPI
MS.gene78281	MS.gene005995	PPI
MS.gene78281	MS.gene54764	PPI
MS.gene78281	MS.gene24806	PPI
MS.gene78281	MS.gene038519	PPI
MS.gene78281	MS.gene27223	PPI
MS.gene78281	MS.gene042783	PPI
MS.gene78281	MS.gene97943	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene78281.t1	MTR_8g039620	66.762	349	108	4	1	342	1	348	4.52e-161	457
MS.gene78281.t1	MTR_3g016210	65.181	359	105	5	1	342	1	356	1.18e-158	452
MS.gene78281.t1	MTR_8g072510	65.407	344	114	4	1	342	1	341	2.65e-152	436
MS.gene78281.t1	MTR_7g021090	63.689	347	109	5	1	342	1	335	6.51e-149	426
MS.gene78281.t1	MTR_1g059930	63.112	347	120	5	1	342	1	344	7.63e-146	419
MS.gene78281.t1	MTR_8g062410	64.451	346	109	5	1	342	1	336	2.69e-142	410
MS.gene78281.t1	MTR_8g073260	60.290	345	126	5	1	342	4	340	3.81e-142	410
MS.gene78281.t1	MTR_7g451420	64.263	319	110	3	1	318	1	316	3.34e-138	395
MS.gene78281.t1	MTR_8g069880	61.834	338	119	4	9	342	7	338	1.95e-136	396
MS.gene78281.t1	MTR_8g068990	59.302	344	117	7	4	342	7	332	1.11e-132	386
MS.gene78281.t1	MTR_8g467780	55.908	347	140	4	7	342	9	353	4.94e-125	367
MS.gene78281.t1	MTR_8g066670	59.942	342	131	3	1	340	1067	1404	2.27e-122	384
MS.gene78281.t1	MTR_8g040930	61.824	296	100	6	52	340	2	291	3.01e-118	347
MS.gene78281.t1	MTR_8g041240	61.824	296	100	6	52	340	2	291	3.01e-118	347
MS.gene78281.t1	MTR_3g105650	50.160	313	144	4	7	311	3	311	1.18e-101	306
MS.gene78281.t1	MTR_8g073275	45.507	345	108	8	52	329	2	333	5.47e-83	259
MS.gene78281.t1	MTR_1g069110	27.795	331	196	11	9	326	18	318	3.24e-29	117
MS.gene78281.t1	MTR_7g099580	25.714	315	181	9	7	316	10	276	3.26e-25	104
MS.gene78281.t1	MTR_7g099570	29.208	202	123	4	2	198	8	194	1.79e-20	92.8
MS.gene78281.t1	MTR_1g115575	26.578	301	164	8	44	311	169	445	1.50e-18	87.0
MS.gene78281.t1	MTR_7g117620	23.875	289	184	7	44	310	119	393	1.51e-15	77.8
MS.gene78281.t1	MTR_1g032100	24.483	290	182	7	45	310	95	371	6.58e-15	75.9
MS.gene78281.t1	MTR_2g101320	28.061	196	115	3	37	230	134	305	3.04e-13	71.2
MS.gene78281.t1	MTR_8g105260	24.742	291	182	8	44	310	101	378	3.34e-13	70.9
MS.gene78281.t1	MTR_7g100430	24.159	327	178	11	42	311	124	437	9.64e-12	66.2
MS.gene78281.t1	MTR_4g062100	22.977	309	210	7	2	310	104	384	1.78e-11	65.5
MS.gene78281.t1	MTR_7g100390	24.567	289	181	8	45	310	88	362	2.88e-11	64.7
MS.gene78281.t1	MTR_2g096190	24.852	169	123	2	2	170	100	264	5.46e-11	63.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene78281.t1	AT4G10720	39.701	335	166	5	8	321	4	323	2.95e-75	238
MS.gene78281.t1	AT4G10720	39.701	335	166	5	8	321	4	323	1.31e-74	237
MS.gene78281.t1	AT5G15500	37.572	346	186	6	5	332	2	335	1.19e-71	230
MS.gene78281.t1	AT5G54620	41.042	307	160	5	15	308	11	309	2.16e-69	223
MS.gene78281.t1	AT1G14480	36.202	337	200	5	8	332	4	337	9.98e-65	211
MS.gene78281.t1	AT1G14500	36.308	325	194	4	8	322	4	325	4.71e-64	209
MS.gene78281.t1	AT5G54610	37.542	301	169	6	15	304	11	303	3.34e-63	207
MS.gene78281.t1	AT1G14480	35.474	327	173	7	8	332	4	294	3.07e-50	172
MS.gene78281.t1	AT5G15500	32.780	241	132	6	110	332	1	229	5.40e-31	120
MS.gene78281.t1	AT5G51160	29.032	279	158	8	62	314	34	298	2.03e-27	112
MS.gene78281.t1	AT4G03500	27.953	254	143	7	71	311	253	479	1.07e-17	84.7
MS.gene78281.t1	AT4G03500	27.953	254	143	7	71	311	253	479	1.07e-17	84.7
MS.gene78281.t1	AT4G03500	27.953	254	143	7	71	311	276	502	1.22e-17	84.3
MS.gene78281.t1	AT3G09550	27.000	300	162	9	45	311	182	457	1.60e-17	84.0
MS.gene78281.t1	AT3G09550	27.000	300	162	9	45	311	182	457	1.60e-17	84.0
MS.gene78281.t1	AT3G09550	27.000	300	162	9	45	311	189	464	1.73e-17	84.0
MS.gene78281.t1	AT3G12360	26.159	302	164	8	44	311	167	443	1.41e-16	81.3
MS.gene78281.t1	AT3G12360	26.159	302	164	8	44	311	155	431	1.60e-16	80.9
MS.gene78281.t1	AT1G34050	24.723	271	162	8	65	314	174	423	7.62e-15	75.9
MS.gene78281.t1	AT5G54700	24.916	297	150	10	24	318	20	245	8.14e-15	75.5
MS.gene78281.t1	AT2G01680	22.093	344	221	7	15	324	58	388	1.85e-14	74.7
MS.gene78281.t1	AT5G54700	27.803	223	121	9	100	318	22	208	2.52e-14	73.6
MS.gene78281.t1	AT2G24600	26.217	267	157	7	79	314	175	432	6.77e-14	73.2
MS.gene78281.t1	AT2G24600	26.217	267	157	7	79	314	175	432	6.77e-14	73.2
MS.gene78281.t1	AT2G24600	24.908	273	153	7	79	314	175	432	1.20e-13	72.4
MS.gene78281.t1	AT2G24600	24.908	273	153	7	79	314	175	432	1.20e-13	72.4
MS.gene78281.t1	AT4G03460	28.910	211	133	4	102	311	292	486	2.38e-13	71.2
MS.gene78281.t1	AT4G03460	28.910	211	133	4	102	311	260	454	2.69e-13	71.2
MS.gene78281.t1	AT5G02620	25.253	297	173	9	45	310	95	373	9.92e-13	69.3
MS.gene78281.t1	AT5G02620	25.253	297	173	9	45	310	95	373	9.92e-13	69.3
MS.gene78281.t1	AT5G02620	25.253	297	173	9	45	310	95	373	9.92e-13	69.3
MS.gene78281.t1	AT1G05640	25.260	289	180	9	44	310	191	465	5.90e-12	67.0
MS.gene78281.t1	AT5G02620	24.684	316	174	9	45	310	95	396	1.25e-11	66.2
MS.gene78281.t1	AT5G02620	24.684	316	174	9	45	310	95	396	1.25e-11	66.2
MS.gene78281.t1	AT2G31820	23.547	327	195	10	21	310	193	501	1.96e-11	65.5
MS.gene78281.t1	AT5G54710	24.262	305	181	9	65	343	187	467	5.00e-11	64.3
MS.gene78281.t1	AT5G54710	24.262	305	181	9	65	343	232	512	5.68e-11	64.3

Find 55 sgRNAs with CRISPR-Local

Find 91 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAGGCCCTTCAAGTACTTCT+TGG	0.237371	6.4:+56907985	MS.gene78281:CDS
TCATAATTTCAGTGGCAAAT+TGG	0.275256	6.4:-56907678	None:intergenic
CTGAAAGCTGCAGCTGTATC+AGG	0.298363	6.4:+56907541	MS.gene78281:CDS
CTTTGCACATCGCTGCATCT+AGG	0.375293	6.4:+56907647	MS.gene78281:CDS
GGGACTCACTATTGATTGAT+AGG	0.376538	6.4:-56908995	None:intergenic
CTCAAGCATTCAATGGATTC+CGG	0.412196	6.4:-56907913	None:intergenic
CCCTTCAAGTACTTCTTGGT+TGG	0.412618	6.4:+56907989	MS.gene78281:CDS
AAAGTGGTCTGCATTCCATT+TGG	0.423506	6.4:-56908272	None:intergenic
TTTGCACATCGCTGCATCTA+GGG	0.428177	6.4:+56907648	MS.gene78281:CDS
AAGCTTTCTCTTTGCTTGTC+CGG	0.429085	6.4:+56907894	MS.gene78281:CDS
TCTCACACTCAAGCATTCAA+TGG	0.429504	6.4:-56907920	None:intergenic
ATTATTACCCGAGGAAATGC+TGG	0.440423	6.4:+56909084	MS.gene78281:CDS
TGCAAAGGAGTTTCAACAAA+TGG	0.447385	6.4:-56907631	None:intergenic
CTTTAGAAGAAATATATTAG+AGG	0.452706	6.4:+56908924	MS.gene78281:CDS
GCTAATTCTTTACTTTAAAG+TGG	0.454482	6.4:-56908288	None:intergenic
TGTCAAGAATGAAAAGTTCC+AGG	0.462154	6.4:+56907966	MS.gene78281:CDS
TTTAGTCACTAAATTCCAAA+TGG	0.468452	6.4:+56908257	MS.gene78281:intron
TTTAGCTTCCAAGAAAATGA+AGG	0.469976	6.4:-56907709	None:intergenic
GGTAATAATAGTAGAATTTG+AGG	0.473471	6.4:-56909070	None:intergenic
AAGAAAATATTATTAAATGT+TGG	0.492021	6.4:+56908796	MS.gene78281:CDS
CGAGAGAGGTGCAAAAGAGC+TGG	0.502941	6.4:+56908026	MS.gene78281:CDS
CCGATCTCCCAGCATTTCCT+CGG	0.503689	6.4:-56909092	None:intergenic
AGAATTAATAAGTGAACCAC+AGG	0.512346	6.4:+56908107	MS.gene78281:CDS
TCAAACATGAACACATATGA+TGG	0.521073	6.4:+56907511	None:intergenic
TCTTGGAAGCTAAATGCTGA+AGG	0.524800	6.4:+56907718	MS.gene78281:CDS
CCGAGGAAATGCTGGGAGAT+CGG	0.529327	6.4:+56909092	MS.gene78281:CDS
ATCCATCTTGCTATGCAAAA+CGG	0.531789	6.4:+56907751	MS.gene78281:CDS
AAAGAACTTGTTAAAGTGCA+AGG	0.532557	6.4:+56907811	MS.gene78281:CDS
GCCAACCAAGAAGTACTTGA+AGG	0.535702	6.4:-56907990	None:intergenic
CTCAAAACAAATTGCGAGAG+AGG	0.539413	6.4:+56908012	MS.gene78281:CDS
GTTAAAGTGCAAGGAAGAGA+AGG	0.540804	6.4:+56907820	MS.gene78281:CDS
ATTTGACTTGCAAAATGAAG+AGG	0.542519	6.4:-56907850	None:intergenic
GCAAAACGGTCAAACAATGA+TGG	0.544713	6.4:+56907765	MS.gene78281:CDS
GTATCACTGACTTCTGAGGC+AGG	0.546924	6.4:-56908826	None:intergenic
CAAGAAGTACTTGAAGGGCC+TGG	0.547051	6.4:-56907984	None:intergenic
CGATCTCCCAGCATTTCCTC+GGG	0.547198	6.4:-56909091	None:intergenic
TTTCAACATCATAATTTCAG+TGG	0.552567	6.4:-56907686	None:intergenic
TTAGACATTGCAACTACAGC+AGG	0.553641	6.4:+56908769	MS.gene78281:CDS
CTAAATCAGAAGGATGAGGC+AGG	0.569601	6.4:+56908060	MS.gene78281:CDS
TTAGCCTGATAAACTCCACC+AGG	0.571244	6.4:-56909018	None:intergenic
TAGAGGAGCAACGTAACACT+TGG	0.572878	6.4:+56908941	MS.gene78281:CDS
TTTGCATAGCAAGATGGATG+GGG	0.573527	6.4:-56907747	None:intergenic
TCTTAGTCTATCTGCAAGTG+TGG	0.575281	6.4:-56908851	None:intergenic
AAACAAAATACTAAATCAGA+AGG	0.579530	6.4:+56908050	MS.gene78281:CDS
AATACTAAATCAGAAGGATG+AGG	0.585811	6.4:+56908056	MS.gene78281:CDS
TTGCACATCGCTGCATCTAG+GGG	0.586343	6.4:+56907649	MS.gene78281:CDS
TTATTACCCGAGGAAATGCT+GGG	0.605752	6.4:+56909085	MS.gene78281:CDS
CTAGATGCAGCGATGTGCAA+AGG	0.614402	6.4:-56907646	None:intergenic
CTTAGTCTATCTGCAAGTGT+GGG	0.615195	6.4:-56908850	None:intergenic
CCAACCAAGAAGTACTTGAA+GGG	0.638063	6.4:-56907989	None:intergenic
GGGAGTATCACTGACTTCTG+AGG	0.643167	6.4:-56908830	None:intergenic
AATTCTACTATTATTACCCG+AGG	0.646609	6.4:+56909075	MS.gene78281:CDS
TAGCCTGATAAACTCCACCA+GGG	0.689510	6.4:-56909017	None:intergenic
AGCCTGATAAACTCCACCAG+GGG	0.703709	6.4:-56909016	None:intergenic
GCCTGATAAACTCCACCAGG+GGG	0.703826	6.4:-56909015	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAGAAAATATTATTAAATGT+TGG	+	chr6.4:56908796-56908815	MS.gene78281:CDS	10.0%
!!	TTTATTTATTTATGATTACT+TGG	+	chr6.4:56908675-56908694	MS.gene78281:intron	10.0%
!!	AAACAAAAGGAAATCATATT+CGG	-	chr6.4:56909153-56909172	None:intergenic	20.0%
!!	AAACAAAATACTAAATCAGA+AGG	+	chr6.4:56908050-56908069	MS.gene78281:CDS	20.0%
!!	AAAGACTTGTATAAATTTGA+AGG	+	chr6.4:56908720-56908739	MS.gene78281:intron	20.0%
!!	AATTAGAAAATAACACATCA+AGG	+	chr6.4:56908192-56908211	MS.gene78281:intron	20.0%
!!!	ATGTGTTATTTTCTAATTGA+GGG	-	chr6.4:56908190-56908209	None:intergenic	20.0%
!!!	CTTTAGAAGAAATATATTAG+AGG	+	chr6.4:56908924-56908943	MS.gene78281:CDS	20.0%
!!!	TTCCTTTTGTTTATCAATTA+TGG	+	chr6.4:56909161-56909180	MS.gene78281:intron	20.0%
!	AAATTTGAAGGCAAAAAACT+TGG	+	chr6.4:56908732-56908751	MS.gene78281:intron	25.0%
!	AATAGTACATTGAAAATACG+TGG	+	chr6.4:56908536-56908555	MS.gene78281:intron	25.0%
!	GAGTTGAAAAAATAAGGAAT+TGG	-	chr6.4:56909131-56909150	None:intergenic	25.0%
!	GGTAATAATAGTAGAATTTG+AGG	-	chr6.4:56909073-56909092	None:intergenic	25.0%
!	TATTCGGAGTTGAAAAAATA+AGG	-	chr6.4:56909137-56909156	None:intergenic	25.0%
!	TCTATATGCTCAAAAATAGA+TGG	-	chr6.4:56907604-56907623	None:intergenic	25.0%
!	TGCCATAATTGATAAACAAA+AGG	-	chr6.4:56909166-56909185	None:intergenic	25.0%
!	TTTAGTCACTAAATTCCAAA+TGG	+	chr6.4:56908257-56908276	MS.gene78281:intron	25.0%
!	TTTCAACATCATAATTTCAG+TGG	-	chr6.4:56907689-56907708	None:intergenic	25.0%
!!	GCTAATTCTTTACTTTAAAG+TGG	-	chr6.4:56908291-56908310	None:intergenic	25.0%
!!	TTTTGTTACAAAATACAACG+TGG	+	chr6.4:56908570-56908589	MS.gene78281:intron	25.0%
!!!	GATGTGTTATTTTCTAATTG+AGG	-	chr6.4:56908191-56908210	None:intergenic	25.0%
!!!	TGTGTTATTTTCTAATTGAG+GGG	-	chr6.4:56908189-56908208	None:intergenic	25.0%
	AAACACTTTGTAAATGACTC+TGG	+	chr6.4:56908220-56908239	MS.gene78281:intron	30.0%
	AATACTAAATCAGAAGGATG+AGG	+	chr6.4:56908056-56908075	MS.gene78281:CDS	30.0%
	AATTCTACTATTATTACCCG+AGG	+	chr6.4:56909075-56909094	MS.gene78281:CDS	30.0%
	AGAATTAATAAGTGAACCAC+AGG	+	chr6.4:56908107-56908126	MS.gene78281:CDS	30.0%
	TAATCTAATGTCATTGCACA+AGG	-	chr6.4:56908136-56908155	None:intergenic	30.0%
	TCATAATTTCAGTGGCAAAT+TGG	-	chr6.4:56907681-56907700	None:intergenic	30.0%
!	CTTCATTTTGCAAGTCAAAT+TGG	+	chr6.4:56907853-56907872	MS.gene78281:CDS	30.0%
!	TGTTGAAACCTTCATTTTCT+TGG	+	chr6.4:56907701-56907720	MS.gene78281:CDS	30.0%
!	TTTAGCTTCCAAGAAAATGA+AGG	-	chr6.4:56907712-56907731	None:intergenic	30.0%
!!	AAAGAACTTGTTAAAGTGCA+AGG	+	chr6.4:56907811-56907830	MS.gene78281:CDS	30.0%
!!	ATTTGACTTGCAAAATGAAG+AGG	-	chr6.4:56907853-56907872	None:intergenic	30.0%
	CATGAACGTTCTCTAACAAA+TGG	+	chr6.4:56908646-56908665	MS.gene78281:intron	35.0%
	TGCAAAGGAGTTTCAACAAA+TGG	-	chr6.4:56907634-56907653	None:intergenic	35.0%
	TGTCAAGAATGAAAAGTTCC+AGG	+	chr6.4:56907966-56907985	MS.gene78281:CDS	35.0%
!	ATCCATCTTGCTATGCAAAA+CGG	+	chr6.4:56907751-56907770	MS.gene78281:CDS	35.0%
!	TGTCACATTTTCTTGTATCG+TGG	-	chr6.4:56908890-56908909	None:intergenic	35.0%
!	TTTTGCAAGTCAAATTGGTG+AGG	+	chr6.4:56907858-56907877	MS.gene78281:CDS	35.0%
!	TTTTGCATAGCAAGATGGAT+GGG	-	chr6.4:56907751-56907770	None:intergenic	35.0%
	AAAGTGGTCTGCATTCCATT+TGG	-	chr6.4:56908275-56908294	None:intergenic	40.0%
	AAGCTTTCTCTTTGCTTGTC+CGG	+	chr6.4:56907894-56907913	MS.gene78281:CDS	40.0%
	ATTATTACCCGAGGAAATGC+TGG	+	chr6.4:56909084-56909103	MS.gene78281:CDS	40.0%
	CTCAAAACAAATTGCGAGAG+AGG	+	chr6.4:56908012-56908031	MS.gene78281:CDS	40.0%
	CTTAGTCTATCTGCAAGTGT+GGG	-	chr6.4:56908853-56908872	None:intergenic	40.0%
	GCAAAACGGTCAAACAATGA+TGG	+	chr6.4:56907765-56907784	MS.gene78281:CDS	40.0%
	GTTAAAGTGCAAGGAAGAGA+AGG	+	chr6.4:56907820-56907839	MS.gene78281:CDS	40.0%
	TCTCACACTCAAGCATTCAA+TGG	-	chr6.4:56907923-56907942	None:intergenic	40.0%
	TCTTAGTCTATCTGCAAGTG+TGG	-	chr6.4:56908854-56908873	None:intergenic	40.0%
	TCTTGGAAGCTAAATGCTGA+AGG	+	chr6.4:56907718-56907737	MS.gene78281:CDS	40.0%
	TTAGACATTGCAACTACAGC+AGG	+	chr6.4:56908769-56908788	MS.gene78281:CDS	40.0%
	TTATTACCCGAGGAAATGCT+GGG	+	chr6.4:56909085-56909104	MS.gene78281:CDS	40.0%
	TTTGCATAGCAAGATGGATG+GGG	-	chr6.4:56907750-56907769	None:intergenic	40.0%
!	ATAGTTTGTCTAACCCCAAC+TGG	+	chr6.4:56909186-56909205	MS.gene78281:intron	40.0%
!	CTCAAGCATTCAATGGATTC+CGG	-	chr6.4:56907916-56907935	None:intergenic	40.0%
!	GGGACTCACTATTGATTGAT+AGG	-	chr6.4:56908998-56909017	None:intergenic	40.0%
!	GTTTTGCATAGCAAGATGGA+TGG	-	chr6.4:56907752-56907771	None:intergenic	40.0%
!	TAGTTTGTCTAACCCCAACT+GGG	+	chr6.4:56909187-56909206	MS.gene78281:intron	40.0%
!!	CCAACCAAGAAGTACTTGAA+GGG	-	chr6.4:56907992-56908011	None:intergenic	40.0%
	CCCTTCAAGTACTTCTTGGT+TGG	+	chr6.4:56907989-56908008	MS.gene78281:CDS	45.0%
	CTAAATCAGAAGGATGAGGC+AGG	+	chr6.4:56908060-56908079	MS.gene78281:CDS	45.0%
	CTAACCCCAACTGGGATAAT+AGG	+	chr6.4:56909195-56909214	MS.gene78281:intron	45.0%
	GGTAGCATAGACTCGATTGT+TGG	+	chr6.4:56908696-56908715	MS.gene78281:intron	45.0%
	TAGAGGAGCAACGTAACACT+TGG	+	chr6.4:56908941-56908960	MS.gene78281:CDS	45.0%
	TAGCCTGATAAACTCCACCA+GGG	-	chr6.4:56909020-56909039	None:intergenic	45.0%
	TCATTGCACAAGGTTACCTG+TGG	-	chr6.4:56908126-56908145	None:intergenic	45.0%
	TTAGCCTGATAAACTCCACC+AGG	-	chr6.4:56909021-56909040	None:intergenic	45.0%
	TTTGCACATCGCTGCATCTA+GGG	+	chr6.4:56907648-56907667	MS.gene78281:CDS	45.0%
!	GACCGTTTTGCATAGCAAGA+TGG	-	chr6.4:56907756-56907775	None:intergenic	45.0%
!!	GCCAACCAAGAAGTACTTGA+AGG	-	chr6.4:56907993-56908012	None:intergenic	45.0%
	ACCCCAACTGGGATAATAGG+CGG	+	chr6.4:56909198-56909217	MS.gene78281:intron	50.0%
	ACCGCCTATTATCCCAGTTG+GGG	-	chr6.4:56909202-56909221	None:intergenic	50.0%
	AGACCGCCTATTATCCCAGT+TGG	-	chr6.4:56909204-56909223	None:intergenic	50.0%
	AGCCTGATAAACTCCACCAG+GGG	-	chr6.4:56909019-56909038	None:intergenic	50.0%
	CAATCAATAGTGAGTCCCCC+TGG	+	chr6.4:56909000-56909019	MS.gene78281:CDS	50.0%
	CAGGCCCTTCAAGTACTTCT+TGG	+	chr6.4:56907985-56908004	MS.gene78281:CDS	50.0%
	CTAGATGCAGCGATGTGCAA+AGG	-	chr6.4:56907649-56907668	None:intergenic	50.0%
	CTGAAAGCTGCAGCTGTATC+AGG	+	chr6.4:56907541-56907560	MS.gene78281:CDS	50.0%
	CTTTGCACATCGCTGCATCT+AGG	+	chr6.4:56907647-56907666	MS.gene78281:CDS	50.0%
	GACCGCCTATTATCCCAGTT+GGG	-	chr6.4:56909203-56909222	None:intergenic	50.0%
	TTGCACATCGCTGCATCTAG+GGG	+	chr6.4:56907649-56907668	MS.gene78281:CDS	50.0%
!!	CAAGAAGTACTTGAAGGGCC+TGG	-	chr6.4:56907987-56908006	None:intergenic	50.0%
!!	GGGAGTATCACTGACTTCTG+AGG	-	chr6.4:56908833-56908852	None:intergenic	50.0%
!!	GTATCACTGACTTCTGAGGC+AGG	-	chr6.4:56908829-56908848	None:intergenic	50.0%
	CCGAGGAAATGCTGGGAGAT+CGG	+	chr6.4:56909092-56909111	MS.gene78281:CDS	55.0%
	CCGATCTCCCAGCATTTCCT+CGG	-	chr6.4:56909095-56909114	None:intergenic	55.0%
	CGATCTCCCAGCATTTCCTC+GGG	-	chr6.4:56909094-56909113	None:intergenic	55.0%
	GCCTGATAAACTCCACCAGG+GGG	-	chr6.4:56909018-56909037	None:intergenic	55.0%
	TCAATAGTGAGTCCCCCTGG+TGG	+	chr6.4:56909003-56909022	MS.gene78281:CDS	55.0%
	TCCCCCTGGTGGAGTTTATC+AGG	+	chr6.4:56909014-56909033	MS.gene78281:CDS	55.0%
!	CGAGAGAGGTGCAAAAGAGC+TGG	+	chr6.4:56908026-56908045	MS.gene78281:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.4	gene	56907517	56909228	56907517	ID=MS.gene78281
chr6.4	mRNA	56907517	56909228	56907517	ID=MS.gene78281.t1;Parent=MS.gene78281
chr6.4	exon	56907517	56908128	56907517	ID=MS.gene78281.t1.exon1;Parent=MS.gene78281.t1
chr6.4	CDS	56907517	56908128	56907517	ID=cds.MS.gene78281.t1;Parent=MS.gene78281.t1
chr6.4	exon	56908262	56908297	56908262	ID=MS.gene78281.t1.exon2;Parent=MS.gene78281.t1
chr6.4	CDS	56908262	56908297	56908262	ID=cds.MS.gene78281.t1;Parent=MS.gene78281.t1
chr6.4	exon	56908742	56909113	56908742	ID=MS.gene78281.t1.exon3;Parent=MS.gene78281.t1
chr6.4	CDS	56908742	56909113	56908742	ID=cds.MS.gene78281.t1;Parent=MS.gene78281.t1
chr6.4	exon	56909217	56909228	56909217	ID=MS.gene78281.t1.exon4;Parent=MS.gene78281.t1
chr6.4	CDS	56909217	56909228	56909217	ID=cds.MS.gene78281.t1;Parent=MS.gene78281.t1

Gene Sequence

>MS.gene78281

ATGAACACATATGATGGTGAGCAGCTGAAAGCTGCAGCTGTATCAGGTGATATAAATCTTCTCTACACTGTAATTCGAAATGATCCATCTATTTTTGAGCATATAGATTTGATACCATTTGTTGAAACTCCTTTGCACATCGCTGCATCTAGGGGTCATCTCCAATTTGCCACTGAAATTATGATGTTGAAACCTTCATTTTCTTGGAAGCTAAATGCTGAAGGATTCAGCCCCATCCATCTTGCTATGCAAAACGGTCAAACAATGATGGTATCTCGCTTTGTTAACATTAATAAAGAACTTGTTAAAGTGCAAGGAAGAGAAGGATTAACTCCTCTTCATTTTGCAAGTCAAATTGGTGAGGTTGATCTTTTAGCAAGCTTTCTCTTTGCTTGTCCGGAATCCATTGAATGCTTGAGTGTGAGACAAGAGACTGCACTGCATATTGCTGTCAAGAATGAAAAGTTCCAGGCCCTTCAAGTACTTCTTGGTTGGCTCAAAACAAATTGCGAGAGAGGTGCAAAAGAGCTGGAAAACAAAATACTAAATCAGAAGGATGAGGCAGGCAATACTATATTGCACATTTCAGCAGAATTAATAAGTGAACCACAGGTAACCTTGTGCAATGACATTAGATTACATGTCTTCTTTATATATTTCTTTTGTTTTCCCCTCAATTAGAAAATAACACATCAAGGAAAAAAAACACTTTGTAAATGACTCTGGTGTTTTTTATTATTTTTAGTCACTAAATTCCAAATGGAATGCAGACCACTTTAAAGTAAAGAATTAGCATGCAAAGAAAAGCACTAGTTTTGATTATTTTTTTTGCATAAAGATGAAGAAAAGTTGTAAATATGTTTTGTAGTAATATAATTCATTTCAGCGTCTTTAAAAAAAAAATTGTAAATAAATAAATAATTTAATATGTTAATAAAAAATTTCTTTAGTCATTATCATAAATGTATAGCTTAGTAAAATTTACTTGAGTTTGACATTAATATTAAATTAAAATAACTAATAGTACATTGAAAATACGTGGTTCTTTTTTTTTTTTGTTACAAAATACAACGTGGTACTTATTTTGTGATATTTTTCTTTTGCAAATGTGTGACACTTAATGTCGTAACATGAACGTTCTCTAACAAATGGTATTTTTTTATTTATTTATGATTACTTGGTAGCATAGACTCGATTGTTGGTAAAGACTTGTATAAATTTGAAGGCAAAAAACTTGGAGAACAAAACAGCATTAGACATTGCAACTACAGCAGGAATCAAGAAAATATTATTAAATGTTGGAGCGAAACCTGCCTCAGAAGTCAGTGATACTCCCACACTTGCAGATAGACTAAGATTAAGATCAAACACCACGATACAAGAAAATGTGACAATTTTTATGAGTCGCTTTAGAAGAAATATATTAGAGGAGCAACGTAACACTTGGTTGATAATTGCGACTCTTGTTGCGACTGCAACCTATCAATCAATAGTGAGTCCCCCTGGTGGAGTTTATCAGGCTAATGCTAGTGACAACACTGTGAACATCACTTCCTCAAATTCTACTATTATTACCCGAGGAAATGCTGGGAGATCGGTATTGTCAACACGCCAATTCCTTATTTTTTCAACTCCGAATATGATTTCCTTTTGTTTATCAATTATGGCAATAGTTTGTCTAACCCCAACTGGGATAATAGGCGGTCTATTAG

Protein sequence

>MS.gene78281.t1

MNTYDGEQLKAAAVSGDINLLYTVIRNDPSIFEHIDLIPFVETPLHIAASRGHLQFATEIMMLKPSFSWKLNAEGFSPIHLAMQNGQTMMVSRFVNINKELVKVQGREGLTPLHFASQIGEVDLLASFLFACPESIECLSVRQETALHIAVKNEKFQALQVLLGWLKTNCERGAKELENKILNQKDEAGNTILHISAELISEPQSLNSKWNADHFKAKNLENKTALDIATTAGIKKILLNVGAKPASEVSDTPTLADRLRLRSNTTIQENVTIFMSRFRRNILEEQRNTWLIIATLVATATYQSIVSPPGGVYQANASDNTVNITSSNSTIITRGNAGRSAVY