AlfalfaGEDB | MS.gene066467

M. sativa cultivar XinJiangDaYe / MS.gene066467

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF12796.10 PF13606.9 PF13857.9 PF13962.9

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066467.t1	XP_003629044.1	88.7	345	37	1	1	345	1	343	9.10E-109	403.7

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066467.t1	Q8GYH5	50.5	303	141	3	11	306	4	304	4.2e-22	107.1

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066467.t1	G7L7W8	88.7	345	37	1	1	345	1	343	6.5e-109	403.7

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
MS.gene005995	MS.gene066467	PPI
MS.gene008767	MS.gene066467	PPI
MS.gene010910	MS.gene066467	PPI
MS.gene010912	MS.gene066467	PPI
MS.gene012203	MS.gene066467	PPI
MS.gene027071	MS.gene066467	PPI
MS.gene027074	MS.gene066467	PPI
MS.gene035805	MS.gene066467	PPI
MS.gene037950	MS.gene066467	PPI
MS.gene039682	MS.gene066467	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066467.t1	MTR_8g072510	88.406	345	38	1	1	345	1	343	0.0	621
MS.gene066467.t1	MTR_8g039620	71.709	357	95	3	1	351	1	357	3.77e-180	506
MS.gene066467.t1	MTR_3g016210	70.000	360	91	3	1	345	1	358	1.77e-177	500
MS.gene066467.t1	MTR_7g021090	70.605	347	85	3	5	345	2	337	1.27e-175	494
MS.gene066467.t1	MTR_8g062410	74.063	347	79	4	1	345	1	338	1.89e-175	495
MS.gene066467.t1	MTR_1g059930	68.696	345	107	1	1	344	1	345	6.18e-166	470
MS.gene066467.t1	MTR_8g068990	65.706	347	102	3	1	344	1	333	4.12e-161	459
MS.gene066467.t1	MTR_7g451420	68.810	311	97	0	5	315	2	312	3.40e-154	436
MS.gene066467.t1	MTR_8g073260	63.953	344	121	2	1	344	1	341	9.29e-153	437
MS.gene066467.t1	MTR_8g069880	65.089	338	116	2	8	344	3	339	8.95e-149	428

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066467.t1	AT4G10720	42.138	318	176	2	11	320	4	321	6.74e-82	255
MS.gene066467.t1	AT4G10720	42.138	318	176	2	11	320	4	321	1.65e-81	255
MS.gene066467.t1	AT1G14480	40.000	325	179	4	11	322	4	325	5.17e-79	249
MS.gene066467.t1	AT1G14500	38.820	322	188	2	11	323	4	325	2.14e-78	247
MS.gene066467.t1	AT5G15500	42.258	310	171	3	12	313	6	315	9.93e-78	246
MS.gene066467.t1	AT5G54620	40.523	306	175	2	11	309	4	309	1.23e-71	229
MS.gene066467.t1	AT5G54610	36.304	303	184	3	11	306	4	304	2.21e-64	211
MS.gene066467.t1	AT1G14480	35.556	315	164	5	11	322	4	282	6.20e-58	193
MS.gene066467.t1	AT5G15500	36.364	209	125	3	113	313	1	209	1.01e-32	125
MS.gene066467.t1	AT5G51160	28.832	274	167	4	66	318	35	301	2.29e-30	120

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Find 69 sgRNAs with CRISPR-Local

Find 90 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATAAAATATTAAACAAA+AGG	0.410662	8.2:-31605958	MS.gene066467:CDS
AAAGACCTTGTTAGAGTTAA+AGG	0.117136	8.2:-31606196	MS.gene066467:CDS
AAATAAAATATTAAACAAAA+GGG	0.459549	8.2:-31605957	MS.gene066467:CDS
AAATACTAGTATAGATTTGA+AGG	0.484650	8.2:-31605761	MS.gene066467:CDS
AACACCACAAACACAAATAA+TGG	0.318375	8.2:-31606496	MS.gene066467:CDS
AACATTTCCTGGGGTAGAAA+TGG	0.413623	8.2:+31605405	None:intergenic
AACCTGATAAATTCCACCGG+GGG	0.670751	8.2:+31605471	None:intergenic
AAGAGTACATTATTAAGTGT+TGG	0.510773	8.2:-31605685	MS.gene066467:CDS
AAGATCAAAGATCACAATGA+TGG	0.485902	8.2:-31605611	MS.gene066467:CDS
AATAATGTACTCTTGATCTC+TGG	0.495445	8.2:+31605694	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAGACCTTGTTAGAGTTAA+AGG	-	chr8.2:31605556-31605575	MS.gene066467:CDS	30.0%
AACACCACAAACACAAATAA+TGG	-	chr8.2:31605256-31605275	MS.gene066467:CDS	30.0%
AAGATCAAAGATCACAATGA+TGG	-	chr8.2:31606141-31606160	MS.gene066467:CDS	30.0%
AATAATGTACTCTTGATCTC+TGG	+	chr8.2:31606061-31606080	None:intergenic	30.0%
AGATTTGAAGGCAAAGAATA+TGG	-	chr8.2:31606003-31606022	MS.gene066467:CDS	30.0%
ATCTCCATTATTTGTGTTTG+TGG	+	chr8.2:31605263-31605282	None:intergenic	30.0%
ATTAAGATTAAGATCACCTG+TGG	+	chr8.2:31605868-31605887	None:intergenic	30.0%
GCAAAACAACAAAAAACGAA+TGG	-	chr8.2:31605510-31605529	MS.gene066467:CDS	30.0%
GTTCATTCTTAACAGCAATA+TGG	+	chr8.2:31605705-31605724	None:intergenic	30.0%
TCAAGAAAAATACACAGAGA+GGG	-	chr8.2:31605758-31605777	MS.gene066467:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8.2	gene	31605253	31606521	31605253	ID=MS.gene066467
chr8.2	mRNA	31605253	31606521	31605253	ID=MS.gene066467.t1;Parent=MS.gene066467
chr8.2	exon	31605904	31606521	31605904	ID=MS.gene066467.t1.exon1;Parent=MS.gene066467.t1
chr8.2	CDS	31605904	31606521	31605904	ID=cds.MS.gene066467.t1;Parent=MS.gene066467.t1
chr8.2	exon	31605385	31605800	31605385	ID=MS.gene066467.t1.exon2;Parent=MS.gene066467.t1
chr8.2	CDS	31605385	31605800	31605385	ID=cds.MS.gene066467.t1;Parent=MS.gene066467.t1
chr8.2	exon	31605253	31605292	31605253	ID=MS.gene066467.t1.exon3;Parent=MS.gene066467.t1
chr8.2	CDS	31605253	31605292	31605253	ID=cds.MS.gene066467.t1;Parent=MS.gene066467.t1

Gene Sequence

>MS.gene066467

ATGAACACCACAAACACAAATAATGGAGATCGATTGAAAGCTGCAGCTGAAGCAGGTGATATAGATCTCCTCTACGCAGTAATTCAAGATGATCCATACGTTTTGGAGCGTATAGATTCAATACCATTTGTTGAAACTCCTTTACATATTGCTGCATCCATGGGTCATATGGACTTTGCCATTGAAATTATGAATTTGAAACCTTCATTTGCTTTGAAACTAAATCCGCAAGGATTCAGCCCCATCCATCTCGCTATGCAAAACAACAAAAAACGAATGGTGTATCACTTTGTGAGCATCAATAAAGACCTTGTTAGAGTTAAAGGAAGAGAAGGCATAACTCCTCTTCATTTTGCAAGTCAAATTAGTGAGGTTAAAATGTTAGCTAACTTTCTCCGTGTGTGTCCGGAATCCATTGAATACTTGACTGTGAGGCGCGAGACTGCACTCCATATTGCTGTTAAGAATGAACAATATGAGGCTCTTCAAGTACTTGTTAGCTGGCTCAAGAAAAATACACAGAGAGGGGCTCAAAAGCTGGAAAATAAAATATTAAACAAAAGGGATAAGGCAGGCAACACCATTTTGCATATTTCAGCACTAAGTAGTGACCCACAGGTGATCTTAATCTTAATCTTATTTATTAACTTATAAGATATAAAATTGCTAACATGAATGATCTCTAACAATTGTATTATTCATTAATTTCTTGGTAGCACAGGCACTTCTATTGTTGGTAAATACTAGTATAGATTTGAAGGCAAAGAATATGGAGAACAAAACCGCATTAGACATAGCAGACACACCAGAGATCAAGAGTACATTATTAAGTGTTGGAGCAAAACCTAGCTTAGAAGTCACTGATTATCCCACATTTGATCATAGAATAAGATCAAAGATCACAATGATGGATGCTCTGGCAATTTATATATATCGCATTACAAGAGACATATCAGAGGAGCAACGTAACACTTGGTTGATAGTTGCGACTCTTGTTGCAACTGCCATCTATCAATCAGCATTGAGTCCCCCCGGTGGAATTTATCAGGTTAGTGCTAGTGATACTAACGTGAACATCACTTCCTCCAATTTTACCATTTCTACCCCAGGAAATGTTGGGAAATCAGTCTTGTCAGGGTATGAATTCTTTTTGTTTTTATTTGTTAATATGTATTCCTTTTCTGTATCAATTTTAGCTATATTTTTTATGATACCATATGGAAAAATAGGCTTTCTAGTGGTTTCTCCAATGGGTTGGTTTACCGCTAG

Protein sequence

>MS.gene066467.t1

MNTTNTNNGDRLKAAAEAGDIDLLYAVIQDDPYVLERIDSIPFVETPLHIAASMGHMDFAIEIMNLKPSFALKLNPQGFSPIHLAMQNNKKRMVYHFVSINKDLVRVKGREGITPLHFASQISEVKMLANFLRVCPESIEYLTVRRETALHIAVKNEQYEALQVLVSWLKKNTQRGAQKLENKILNKRDKAGNTILHISALSSDPQALLLLVNTSIDLKAKNMENKTALDIADTPEIKSTLLSVGAKPSLEVTDYPTFDHRIRSKITMMDALAIYIYRITRDISEEQRNTWLIVATLVATAIYQSALSPPGGIYQVSASDTNVNITSSNFTISTPGNVGKSVLSGLSSGFSNGLVYR