AlfalfaGEDB | MS.gene23852

M. sativa cultivar XinJiangDaYe / MS.gene23852

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF12796.10 PF13857.9 PF13962.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23852.t1	XP_003629044.1	78.7	348	72	1	1	348	1	346	1.90E-110	409.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23852.t1	Q8GYH5	40.3	303	172	3	11	306	4	304	2.8e-22	107.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23852.t1	G7L7W8	78.7	348	72	1	1	348	1	346	1.4e-110	409.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene23852	MS.gene014931	PPI
MS.gene23852	MS.gene21888	PPI
MS.gene30172	MS.gene23852	PPI
MS.gene23852	MS.gene97379	PPI
MS.gene23852	MS.gene066500	PPI
MS.gene23852	MS.gene39623	PPI
MS.gene23852	MS.gene36842	PPI
MS.gene027071	MS.gene23852	PPI
MS.gene23852	MS.gene066464	PPI
MS.gene23852	MS.gene07706	PPI
MS.gene23852	MS.gene071113	PPI
MS.gene23852	MS.gene90400	PPI
MS.gene23852	MS.gene022109	PPI
MS.gene010910	MS.gene23852	PPI
MS.gene23852	MS.gene041770	PPI
MS.gene23852	MS.gene81055	PPI
MS.gene23852	MS.gene65775	PPI
MS.gene23852	MS.gene99554	PPI
MS.gene23852	MS.gene80163	PPI
MS.gene063222	MS.gene23852	PPI
MS.gene23852	MS.gene02469	PPI
MS.gene23852	MS.gene33377	PPI
MS.gene23852	MS.gene51335	PPI
MS.gene23852	MS.gene045937	PPI
MS.gene23852	MS.gene027072	PPI
MS.gene69687	MS.gene23852	PPI
MS.gene010912	MS.gene23852	PPI
MS.gene23852	MS.gene51278	PPI
MS.gene23852	MS.gene69966	PPI
MS.gene23852	MS.gene87218	PPI
MS.gene78281	MS.gene23852	PPI
MS.gene23852	MS.gene025229	PPI
MS.gene23852	MS.gene30433	PPI
MS.gene23852	MS.gene066501	PPI
MS.gene23852	MS.gene043742	PPI
MS.gene23852	MS.gene062662	PPI
MS.gene23852	MS.gene027441	PPI
MS.gene23852	MS.gene21734	PPI
MS.gene23852	MS.gene30177	PPI
MS.gene23852	MS.gene70008	PPI
MS.gene23852	MS.gene008767	PPI
MS.gene23852	MS.gene008833	PPI
MS.gene23852	MS.gene67157	PPI
MS.gene23852	MS.gene23811	PPI
MS.gene23852	MS.gene25244	PPI
MS.gene23852	MS.gene69965	PPI
MS.gene23852	MS.gene82740	PPI
MS.gene23852	MS.gene051003	PPI
MS.gene23852	MS.gene48655	PPI
MS.gene23852	MS.gene09785	PPI
MS.gene23852	MS.gene64441	PPI
MS.gene23852	MS.gene066046	PPI
MS.gene23852	MS.gene007486	PPI
MS.gene23852	MS.gene78281	PPI
MS.gene23852	MS.gene035805	PPI
MS.gene23852	MS.gene23810	PPI
MS.gene23852	MS.gene08470	PPI
MS.gene23852	MS.gene027073	PPI
MS.gene23852	MS.gene34712	PPI
MS.gene23852	MS.gene65463	PPI
MS.gene23852	MS.gene007485	PPI
MS.gene47479	MS.gene23852	PPI
MS.gene23852	MS.gene007487	PPI
MS.gene23852	MS.gene037184	PPI
MS.gene23852	MS.gene23196	PPI
MS.gene23852	MS.gene039682	PPI
MS.gene23852	MS.gene067705	PPI
MS.gene23852	MS.gene71204	PPI
MS.gene23852	MS.gene41291	PPI
MS.gene23852	MS.gene027075	PPI
MS.gene23852	MS.gene061932	PPI
MS.gene23852	MS.gene050457	PPI
MS.gene23852	MS.gene99973	PPI
MS.gene23852	MS.gene30174	PPI
MS.gene23852	MS.gene26021	PPI
MS.gene23852	MS.gene064542	PPI
MS.gene23852	MS.gene91775	PPI
MS.gene23852	MS.gene25515	PPI
MS.gene23852	MS.gene34651	PPI
MS.gene23852	MS.gene056455	PPI
MS.gene23852	MS.gene89986	PPI
MS.gene039682	MS.gene23852	PPI
MS.gene23852	MS.gene22482	PPI
MS.gene23852	MS.gene050270	PPI
MS.gene23852	MS.gene60920	PPI
MS.gene23852	MS.gene058128	PPI
MS.gene23852	MS.gene34009	PPI
MS.gene23852	MS.gene018518	PPI
MS.gene23852	MS.gene043662	PPI
MS.gene23852	MS.gene065848	PPI
MS.gene23852	MS.gene004844	PPI
MS.gene23852	MS.gene068249	PPI
MS.gene23852	MS.gene99588	PPI
MS.gene23852	MS.gene005995	PPI
MS.gene23852	MS.gene54764	PPI
MS.gene23852	MS.gene24806	PPI
MS.gene23852	MS.gene038519	PPI
MS.gene23852	MS.gene27223	PPI
MS.gene23852	MS.gene042783	PPI
MS.gene23852	MS.gene25024	PPI
MS.gene23852	MS.gene012203	PPI
MS.gene23852	MS.gene26019	PPI
MS.gene23852	MS.gene97943	PPI
MS.gene23852	MS.gene050272	PPI
MS.gene23852	MS.gene63085	PPI
MS.gene23852	MS.gene69687	PPI
MS.gene23852	MS.gene26532	PPI
MS.gene23852	MS.gene050456	PPI
MS.gene23852	MS.gene30176	PPI
MS.gene056455	MS.gene23852	PPI
MS.gene23852	MS.gene51258	PPI
MS.gene23852	MS.gene32723	PPI
MS.gene23852	MS.gene010910	PPI
MS.gene23852	MS.gene053363	PPI
MS.gene23852	MS.gene066467	PPI
MS.gene23852	MS.gene024753	PPI
MS.gene23852	MS.gene32726	PPI
MS.gene23852	MS.gene71122	PPI
MS.gene69965	MS.gene23852	PPI
MS.gene23852	MS.gene24922	PPI
MS.gene23852	MS.gene052956	PPI
MS.gene23852	MS.gene73119	PPI
MS.gene23852	MS.gene92243	PPI
MS.gene23852	MS.gene85534	PPI
MS.gene23852	MS.gene037950	PPI
MS.gene23852	MS.gene027074	PPI
MS.gene23852	MS.gene51262	PPI
MS.gene23852	MS.gene23881	PPI
MS.gene23852	MS.gene26672	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23852.t1	MTR_8g072510	86.158	354	46	2	1	353	1	352	0.0	610
MS.gene23852.t1	MTR_8g039620	69.482	367	100	3	1	355	1	367	1.06e-177	503
MS.gene23852.t1	MTR_8g062410	72.857	350	84	4	1	348	1	341	8.72e-171	486
MS.gene23852.t1	MTR_3g016210	68.595	363	97	3	1	348	1	361	4.62e-170	483
MS.gene23852.t1	MTR_7g021090	69.143	350	100	4	5	353	2	344	1.29e-167	476
MS.gene23852.t1	MTR_1g059930	67.908	349	111	1	1	348	1	349	1.27e-162	464
MS.gene23852.t1	MTR_8g068990	64.943	348	103	4	1	344	1	333	1.42e-156	450
MS.gene23852.t1	MTR_8g073260	60.388	361	132	3	1	353	1	358	8.96e-149	429
MS.gene23852.t1	MTR_7g451420	67.524	311	101	0	5	315	2	312	3.70e-148	423
MS.gene23852.t1	MTR_8g069880	65.130	347	118	3	8	352	3	348	4.23e-147	426
MS.gene23852.t1	MTR_8g040930	66.779	298	96	1	54	348	1	298	5.19e-138	400
MS.gene23852.t1	MTR_8g041240	66.779	298	96	1	54	348	1	298	5.19e-138	400
MS.gene23852.t1	MTR_8g066670	54.717	424	168	7	1	405	1067	1485	4.36e-133	416
MS.gene23852.t1	MTR_8g467780	58.000	350	136	3	10	348	9	358	5.57e-131	384
MS.gene23852.t1	MTR_3g105650	51.948	308	140	2	12	312	5	311	1.45e-102	311
MS.gene23852.t1	MTR_8g073275	45.304	362	121	7	54	348	1	352	9.01e-88	274
MS.gene23852.t1	MTR_1g069110	27.171	357	217	11	12	357	18	342	6.72e-27	112
MS.gene23852.t1	MTR_7g099580	25.589	297	170	8	24	315	24	274	2.21e-22	97.8
MS.gene23852.t1	MTR_2g101320	28.704	216	136	4	43	250	137	342	8.56e-20	92.0
MS.gene23852.t1	MTR_7g099570	28.866	194	124	3	12	200	9	193	1.54e-19	91.3
MS.gene23852.t1	MTR_2g101310	30.233	215	132	4	43	249	137	341	4.04e-19	90.1
MS.gene23852.t1	MTR_2g101310	30.233	215	132	4	43	249	137	341	4.85e-19	89.4
MS.gene23852.t1	MTR_2g096190	23.867	331	215	6	5	311	63	380	6.51e-16	80.1
MS.gene23852.t1	MTR_1g115575	25.688	327	195	9	15	312	138	445	1.91e-15	78.6
MS.gene23852.t1	MTR_7g117620	24.739	287	182	6	47	311	119	393	2.83e-15	78.2
MS.gene23852.t1	MTR_4g062100	23.636	330	203	8	12	311	74	384	5.76e-15	77.0
MS.gene23852.t1	MTR_8g105260	27.273	209	132	3	47	247	101	297	2.44e-13	72.0
MS.gene23852.t1	MTR_1g032100	22.759	290	187	5	47	311	94	371	4.31e-13	71.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23852.t1	AT4G10720	42.453	318	175	2	11	320	4	321	1.54e-81	256
MS.gene23852.t1	AT4G10720	42.453	318	175	2	11	320	4	321	3.49e-81	256
MS.gene23852.t1	AT1G14500	40.062	322	184	2	11	323	4	325	1.04e-79	252
MS.gene23852.t1	AT1G14480	36.318	402	238	6	11	395	4	404	1.32e-77	247
MS.gene23852.t1	AT5G15500	41.935	310	172	3	12	313	6	315	2.58e-75	242
MS.gene23852.t1	AT5G54620	41.368	307	173	2	10	309	3	309	4.07e-72	233
MS.gene23852.t1	AT5G54610	37.954	303	179	3	11	306	4	304	1.12e-64	213
MS.gene23852.t1	AT1G14480	33.163	392	221	7	11	395	4	361	9.00e-58	194
MS.gene23852.t1	AT5G15500	36.842	209	124	3	113	313	1	209	1.43e-31	123
MS.gene23852.t1	AT5G51160	29.197	274	166	4	66	318	35	301	2.15e-27	113
MS.gene23852.t1	AT4G03460	31.628	215	124	5	103	312	290	486	3.04e-18	87.4
MS.gene23852.t1	AT4G03460	31.628	215	124	5	103	312	258	454	3.30e-18	87.0
MS.gene23852.t1	AT4G03500	30.275	218	128	6	101	312	303	502	9.44e-18	85.9
MS.gene23852.t1	AT4G03500	30.275	218	128	6	101	312	280	479	9.52e-18	85.9
MS.gene23852.t1	AT4G03500	30.275	218	128	6	101	312	280	479	9.52e-18	85.9
MS.gene23852.t1	AT5G54700	27.815	302	149	13	27	327	20	253	2.88e-17	84.0
MS.gene23852.t1	AT1G07710	25.697	323	204	7	12	311	70	379	1.75e-15	78.6
MS.gene23852.t1	AT1G07710	25.697	323	204	7	12	311	70	379	1.75e-15	78.6
MS.gene23852.t1	AT1G34050	26.000	300	187	10	34	315	141	423	2.68e-15	78.2
MS.gene23852.t1	AT2G01680	24.437	311	184	7	47	325	97	388	2.76e-15	78.2
MS.gene23852.t1	AT4G03490	27.812	320	170	14	12	323	211	477	9.66e-15	76.6
MS.gene23852.t1	AT4G03490	27.187	320	172	12	12	323	201	467	1.43e-14	75.9
MS.gene23852.t1	AT4G03490	27.187	320	172	12	12	323	211	477	1.45e-14	75.9
MS.gene23852.t1	AT1G10340	27.848	237	129	7	112	315	201	428	1.27e-13	73.2
MS.gene23852.t1	AT1G10340	27.848	237	129	7	112	315	205	432	1.39e-13	72.8
MS.gene23852.t1	AT1G03670	28.000	225	127	6	104	318	279	478	1.51e-13	72.8
MS.gene23852.t1	AT1G05640	25.694	288	178	8	47	311	191	465	3.96e-13	71.6
MS.gene23852.t1	AT4G03480	23.823	361	187	11	12	358	360	646	4.27e-13	71.6
MS.gene23852.t1	AT4G03480	23.823	361	187	11	12	358	357	643	5.18e-13	71.2
MS.gene23852.t1	AT3G12360	25.000	292	178	6	47	312	155	431	4.05e-12	68.2
MS.gene23852.t1	AT3G12360	25.000	292	178	6	47	312	167	443	4.74e-12	68.2
MS.gene23852.t1	AT3G09550	25.153	326	198	9	15	312	150	457	6.65e-12	67.8
MS.gene23852.t1	AT3G09550	25.153	326	198	9	15	312	150	457	6.65e-12	67.8
MS.gene23852.t1	AT4G03450	26.059	307	172	11	12	312	227	484	6.93e-12	67.8
MS.gene23852.t1	AT3G09550	25.153	326	198	9	15	312	157	464	7.00e-12	67.8
MS.gene23852.t1	AT4G03450	26.059	307	172	11	12	312	227	484	7.32e-12	67.8

Find 98 sgRNAs with CRISPR-Local

Find 194 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGACCTTGTTAGAGTTAA+AGG	0.117136	8.4:-31690550	MS.gene23852:CDS
TCAAGATGATCCATACATTT+TGG	0.169438	8.4:-31690770	MS.gene23852:CDS
AATTGTGGTGGTTGGGATAA+AGG	0.222495	8.4:+31688230	None:intergenic
CTTCATTATGCAAGTGAAAT+TGG	0.222947	8.4:-31690508	MS.gene23852:CDS
CCTTCATGGTCGGCAGCGGC+TGG	0.259348	8.4:+31688065	None:intergenic
TGTTAGCTTACTTTCTCTTT+TGG	0.259432	8.4:-31690474	MS.gene23852:CDS
GTCAAGTATTCAATGGATTC+TGG	0.306997	8.4:+31690448	None:intergenic
GCAAAATAACCAAGAACTTA+TGG	0.309064	8.4:-31690596	MS.gene23852:CDS
TGATCAAATGTGGGATAATC+AGG	0.311341	8.4:+31689938	None:intergenic
AACACCACAAACACAAATAA+TGG	0.318375	8.4:-31690850	MS.gene23852:CDS
CTCTCCATTATTTGTGTTTG+TGG	0.320623	8.4:+31690846	None:intergenic
TGGGGTAGAAATGGTAAAAT+TGG	0.321057	8.4:+31689712	None:intergenic
ACTGATTTCCCAACATTTCC+TGG	0.324820	8.4:+31689692	None:intergenic
GTTCATTCTTAACAGCAATA+TGG	0.326006	8.4:+31690404	None:intergenic
AGCGATACACCATAAGTTCT+TGG	0.327799	8.4:+31690587	None:intergenic
ATTATCCCACATTTGATCAT+AGG	0.334358	8.4:-31689934	MS.gene23852:CDS
ACAAATTACCATCTCCTTGT+TGG	0.371436	8.4:-31688203	MS.gene23852:CDS
AGATTTGAAGGCAAAGAATA+TGG	0.376286	8.4:-31690047	MS.gene23852:CDS
TTGGCTGGCCGCAGCTCCTC+AGG	0.382440	8.4:-31688184	MS.gene23852:CDS
CTTCATGGTCGGCAGCGGCT+GGG	0.383341	8.4:+31688066	None:intergenic
TTTACATATTGCTGCATCCA+TGG	0.394377	8.4:-31690713	MS.gene23852:CDS
TTTCAAATTCATAATTTCAA+TGG	0.401833	8.4:+31690675	None:intergenic
GCGAAGAGGGGCTAAAGATC+TGG	0.403132	8.4:-31690335	MS.gene23852:CDS
TTCATGGTCGGCAGCGGCTG+GGG	0.407412	8.4:+31688067	None:intergenic
AACATTTCCTGGGGTAGAAA+TGG	0.413623	8.4:+31689703	None:intergenic
TAGTGCTGAAATGTGCAAAA+TGG	0.415741	8.4:+31690273	None:intergenic
CTCTTCAAGTACTTGTTAGC+TGG	0.417708	8.4:-31690372	MS.gene23852:CDS
CAACCACCCTCTTGTTGAAT+CGG	0.438278	8.4:-31688145	MS.gene23852:CDS
AGATCAAAGATCACAACTAT+AGG	0.438703	8.4:-31689908	MS.gene23852:CDS
TCTATACGCTCCAAAATGTA+TGG	0.444576	8.4:+31690760	None:intergenic
AATATTAAACAGAAGGGATA+AGG	0.446119	8.4:-31690305	MS.gene23852:CDS
TGTAAAGGAGTTTCAACAAA+TGG	0.448723	8.4:+31690730	None:intergenic
GTTGGGAAATCAGTCTTGTC+GGG	0.453796	8.4:-31689683	MS.gene23852:CDS
TTTAGCTTCAAAGCAAATGA+AGG	0.453815	8.4:+31690652	None:intergenic
TTGAAAGCTGCAGCTGAAGC+AGG	0.454553	8.4:-31690820	MS.gene23852:CDS
ACACAGGCAGTTCTATTGTT+GGG	0.467417	8.4:-31690082	MS.gene23852:intron
GTGGTTGGGATAAAGGAAAA+AGG	0.476299	8.4:+31688237	None:intergenic
ACTCAATGCTGATTGATAGA+TGG	0.480622	8.4:+31689793	None:intergenic
AACACAGGCAGTTCTATTGT+TGG	0.484666	8.4:-31690083	MS.gene23852:intron
TGTTGGGAAATCAGTCTTGT+CGG	0.490536	8.4:-31689684	MS.gene23852:CDS
AATAATATACTCTTGATCTC+TGG	0.490722	8.4:+31689992	None:intergenic
GGAAAACGAAAGTAAGAATG+AGG	0.492469	8.4:+31688112	None:intergenic
TTTCTACCCCAGGAAATGTT+GGG	0.494162	8.4:-31689700	MS.gene23852:CDS
ATTACCATCTCCTTGTTGGC+TGG	0.497421	8.4:-31688199	MS.gene23852:CDS
CTGATTTCCCAACATTTCCT+GGG	0.500204	8.4:+31689693	None:intergenic
CCTCACTGTCAAGTATTCAA+TGG	0.501652	8.4:+31690441	None:intergenic
ATTTCTACCCCAGGAAATGT+TGG	0.503031	8.4:-31689701	MS.gene23852:CDS
AAGTACTAGTATAGATTTGA+AGG	0.503292	8.4:-31690059	MS.gene23852:CDS
GTCTCTTACTCCTTCATGGT+CGG	0.503432	8.4:+31688055	None:intergenic
GTTAGAGTTAAAGGCAGAGA+AGG	0.503819	8.4:-31690541	MS.gene23852:CDS
AGAGGGTGGTTGCATGGTTG+TGG	0.504038	8.4:+31688156	None:intergenic
TGTTAAGAATGAACAATATG+AGG	0.511815	8.4:-31690395	MS.gene23852:CDS
GAAAACGAAAGTAAGAATGA+GGG	0.511944	8.4:+31688113	None:intergenic
GGAGCTGCGGCCAGCCAACA+AGG	0.523073	8.4:+31688189	None:intergenic
TTACATATTGCTGCATCCAT+GGG	0.527095	8.4:-31690712	MS.gene23852:CDS
AAGAGTATATTATTAAGCGT+TGG	0.530456	8.4:-31689983	MS.gene23852:CDS
AAAATAAAATATTAAACAGA+AGG	0.532425	8.4:-31690312	MS.gene23852:CDS
GAAAGTAAGAATGAGGGAGA+TGG	0.543265	8.4:+31688119	None:intergenic
GGTAGAAATGGTAAAATTGG+AGG	0.549602	8.4:+31689715	None:intergenic
CTCTGCCTTTAACTCTAACA+AGG	0.552932	8.4:+31690545	None:intergenic
TCAACAAGAGGGTGGTTGCA+TGG	0.553738	8.4:+31688150	None:intergenic
TCTTATCCTATGATCAAATG+TGG	0.558012	8.4:+31689928	None:intergenic
TACTCCTTCATGGTCGGCAG+CGG	0.558828	8.4:+31688061	None:intergenic
AAATAAAATATTAAACAGAA+GGG	0.562493	8.4:-31690311	MS.gene23852:CDS
CAGAGGAGCAACGTAACACT+TGG	0.565419	8.4:-31689844	MS.gene23852:CDS
ATTTCACTTGCATAATGAAG+AGG	0.565575	8.4:+31690511	None:intergenic
CATGGTTGTGGCGGTGCCTG+AGG	0.567452	8.4:+31688168	None:intergenic
GGCAAAGTCAATATGACCCA+TGG	0.572455	8.4:+31690696	None:intergenic
ATCCCGCAACAGAAAACATA+GGG	0.573766	8.4:+31688091	None:intergenic
TTAGAGTTAAAGGCAGAGAA+GGG	0.575623	8.4:-31690540	MS.gene23852:CDS
ATGGATGCAGCAATATGTAA+AGG	0.578412	8.4:+31690715	None:intergenic
TTTGCATTGCGAGATGGATG+GGG	0.578706	8.4:+31690614	None:intergenic
CCAGCCGCTGCCGACCATGA+AGG	0.582850	8.4:-31688065	None:intergenic
GCGGCCAGCCAACAAGGAGA+TGG	0.585009	8.4:+31688195	None:intergenic
TATCCCGCAACAGAAAACAT+AGG	0.591189	8.4:+31688090	None:intergenic
TGGCGGTGCCTGAGGAGCTG+CGG	0.591353	8.4:+31688176	None:intergenic
CATCTTGAATTACTGTGTAG+AGG	0.592515	8.4:+31690785	None:intergenic
GATCAAATGTGGGATAATCA+GGG	0.593956	8.4:+31689939	None:intergenic
TTAAACAGAAGGGATAAGGC+AGG	0.594049	8.4:-31690301	MS.gene23852:CDS
TAATCAGGGACTGCTAAGCT+AGG	0.602803	8.4:+31689953	None:intergenic
GGGTGGTTGCATGGTTGTGG+CGG	0.604902	8.4:+31688159	None:intergenic
CTAACCTGATAAATTCCACC+GGG	0.607279	8.4:+31689767	None:intergenic
ATGGAGTCCGATTCAACAAG+AGG	0.610452	8.4:+31688138	None:intergenic
AGCACTAAGTAGTGACCCAC+AGG	0.611178	8.4:-31690257	MS.gene23852:intron
ACTAACCTGATAAATTCCAC+CGG	0.617401	8.4:+31689766	None:intergenic
TTATGCAAGTGAAATTGGTG+AGG	0.624400	8.4:-31690503	MS.gene23852:CDS
CTTATCCTATGATCAAATGT+GGG	0.628873	8.4:+31689929	None:intergenic
TGGAGTCCGATTCAACAAGA+GGG	0.657435	8.4:+31688139	None:intergenic
GCTTTGAAGCTAAATCCGCA+AGG	0.665685	8.4:-31690643	MS.gene23852:CDS
TGATTTCCCAACATTTCCTG+GGG	0.666728	8.4:+31689694	None:intergenic
TGGATGGGGCTGAATCCTTG+CGG	0.668802	8.4:+31690628	None:intergenic
AACCTGATAAATTCCACCGG+GGG	0.670751	8.4:+31689769	None:intergenic
TTGGGAAATCAGTCTTGTCG+GGG	0.672939	8.4:-31689682	MS.gene23852:intron
CCATTGAATACTTGACAGTG+AGG	0.697151	8.4:-31690441	MS.gene23852:CDS
ACCTGATAAATTCCACCGGG+GGG	0.705401	8.4:+31689770	None:intergenic
AGTCCGATTCAACAAGAGGG+TGG	0.709184	8.4:+31688142	None:intergenic
CATTACAACAGACATATCAG+AGG	0.718709	8.4:-31689861	MS.gene23852:CDS
TAACCTGATAAATTCCACCG+GGG	0.729886	8.4:+31689768	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAATAAAATATTAAACAGA+AGG	-	chr8.4:31688609-31688628	MS.gene23852:intron	10.0%
!!	AAATAAAATATTAAACAGAA+GGG	-	chr8.4:31688610-31688629	MS.gene23852:intron	10.0%
!!!	TATCTTATTTATATATAAGT+TGG	+	chr8.4:31688762-31688781	None:intergenic	10.0%
!!	TGTATAATTATAAGTGTATA+TGG	-	chr8.4:31690292-31690311	MS.gene23852:CDS	15.0%
!!	TGTATTATTCATTAATTTCT+TGG	-	chr8.4:31688814-31688833	MS.gene23852:intron	15.0%
!!	TTTCAAATTCATAATTTCAA+TGG	+	chr8.4:31688249-31688268	None:intergenic	15.0%
!!!	ATATTTTTTATGATACCATA+TGG	-	chr8.4:31689322-31689341	MS.gene23852:intron	15.0%
!!!	TATTTATATATAAGTTGGTA+GGG	+	chr8.4:31688757-31688776	None:intergenic	15.0%
!!!	TTATTTATATATAAGTTGGT+AGG	+	chr8.4:31688758-31688777	None:intergenic	15.0%
!!	CATAGAAAATAAATAACTAG+CGG	+	chr8.4:31689389-31689408	None:intergenic	20.0%
!!!	ATTTATATATAAGTTGGTAG+GGG	+	chr8.4:31688756-31688775	None:intergenic	20.0%
!!!	CATATCATGTATTTTATTGA+AGG	-	chr8.4:31689963-31689982	MS.gene23852:CDS	20.0%
!!!	CTAGTTATTTATTTTCTATG+TGG	-	chr8.4:31689389-31689408	MS.gene23852:intron	20.0%
!	AAGTACTAGTATAGATTTGA+AGG	-	chr8.4:31688862-31688881	MS.gene23852:intron	25.0%
!	AATAATATACTCTTGATCTC+TGG	+	chr8.4:31688932-31688951	None:intergenic	25.0%
!	AATATTAAACAGAAGGGATA+AGG	-	chr8.4:31688616-31688635	MS.gene23852:intron	25.0%
!	ACATCAACAAATTAAGGTTA+TGG	-	chr8.4:31689753-31689772	MS.gene23852:CDS	25.0%
!	AGAGAGAATATTATTCCATA+TGG	+	chr8.4:31689340-31689359	None:intergenic	25.0%
!	AGCTAAAATTGATACAGAAA+AGG	+	chr8.4:31689302-31689321	None:intergenic	25.0%
!	ATAAGATTAAGATTACCTGT+GGG	+	chr8.4:31688682-31688701	None:intergenic	25.0%
!	TAGTTCATTTCTGTTATACA+TGG	-	chr8.4:31689459-31689478	MS.gene23852:intron	25.0%
!	TGTTAAGAATGAACAATATG+AGG	-	chr8.4:31688526-31688545	MS.gene23852:intron	25.0%
!!	AAGAGTATATTATTAAGCGT+TGG	-	chr8.4:31688938-31688957	MS.gene23852:intron	25.0%
!!	ACTCTTTGTATTGATGTAAA+TGG	+	chr8.4:31689668-31689687	None:intergenic	25.0%
!!!	ACTCACATTAGTTTGTTTAT+CGG	-	chr8.4:31690442-31690461	MS.gene23852:CDS	25.0%
!!!	CACAGTTGACAAATTTTAAT+TGG	-	chr8.4:31690031-31690050	MS.gene23852:CDS	25.0%
!!!	CACATGTCATTTTTTTATGT+GGG	+	chr8.4:31690595-31690614	None:intergenic	25.0%
!!!	GTTATTTATTTTCTATGTGG+AGG	-	chr8.4:31689392-31689411	MS.gene23852:intron	25.0%
!!!	TAATTTGTTTTCAATTGTGG+TGG	+	chr8.4:31690706-31690725	None:intergenic	25.0%
!!!	TGGTAATTTGTTTTCAATTG+TGG	+	chr8.4:31690709-31690728	None:intergenic	25.0%
	AAAAAGGAAAAGAGAAGCTA+GGG	+	chr8.4:31690671-31690690	None:intergenic	30.0%
	AAAAATGACATGTGGCAATA+TGG	-	chr8.4:31690601-31690620	MS.gene23852:CDS	30.0%
	AAAATGACATGTGGCAATAT+GGG	-	chr8.4:31690602-31690621	MS.gene23852:CDS	30.0%
	AAAGACCTTGTTAGAGTTAA+AGG	-	chr8.4:31688371-31688390	MS.gene23852:intron	30.0%
	AAATCTAAAGAGTCCTTGAT+AGG	-	chr8.4:31690536-31690555	MS.gene23852:CDS	30.0%
	AACACCACAAACACAAATAA+TGG	-	chr8.4:31688071-31688090	MS.gene23852:CDS	30.0%
	AGATCAAAGATCACAACTAT+AGG	-	chr8.4:31689013-31689032	MS.gene23852:intron	30.0%
	AGATTTGAAGGCAAAGAATA+TGG	-	chr8.4:31688874-31688893	MS.gene23852:intron	30.0%
	ATTATCCCACATTTGATCAT+AGG	-	chr8.4:31688987-31689006	MS.gene23852:intron	30.0%
	ATTTCACTTGCATAATGAAG+AGG	+	chr8.4:31688413-31688432	None:intergenic	30.0%
	CATAAGATTAAGATTACCTG+TGG	+	chr8.4:31688683-31688702	None:intergenic	30.0%
	CCACATAAAAAAATGACATG+TGG	-	chr8.4:31690593-31690612	MS.gene23852:CDS	30.0%
	CTTATCCTATGATCAAATGT+GGG	+	chr8.4:31688995-31689014	None:intergenic	30.0%
	CTTCATTATGCAAGTGAAAT+TGG	-	chr8.4:31688413-31688432	MS.gene23852:intron	30.0%
	GAAAACGAAAGTAAGAATGA+GGG	+	chr8.4:31690811-31690830	None:intergenic	30.0%
	GCAAAATAACCAAGAACTTA+TGG	-	chr8.4:31688325-31688344	MS.gene23852:intron	30.0%
	GGAGAAACATCAACAAATTA+AGG	-	chr8.4:31689747-31689766	MS.gene23852:CDS	30.0%
	GTTCATTCTTAACAGCAATA+TGG	+	chr8.4:31688520-31688539	None:intergenic	30.0%
	TCAAAAAAAATACGCGAAGA+GGG	-	chr8.4:31688573-31688592	MS.gene23852:intron	30.0%
	TCTTATCCTATGATCAAATG+TGG	+	chr8.4:31688996-31689015	None:intergenic	30.0%
	TGGAATAATATTCTCTCTAG+TGG	-	chr8.4:31689342-31689361	MS.gene23852:intron	30.0%
	TGTAAAGGAGTTTCAACAAA+TGG	+	chr8.4:31688194-31688213	None:intergenic	30.0%
!	CATGATAATGCTATTATGTG+AGG	+	chr8.4:31690274-31690293	None:intergenic	30.0%
!	CATTAATTTCTTGGTAACAC+AGG	-	chr8.4:31688823-31688842	MS.gene23852:intron	30.0%
!	TCAAGATGATCCATACATTT+TGG	-	chr8.4:31688151-31688170	MS.gene23852:CDS	30.0%
!	TTGCTAAGCATTGTTTCAAT+AGG	-	chr8.4:31689726-31689745	MS.gene23852:CDS	30.0%
!	TTTAGCTTCAAAGCAAATGA+AGG	+	chr8.4:31688272-31688291	None:intergenic	30.0%
!!	GAGACATGTGATTTTAGTAA+TGG	+	chr8.4:31688706-31688725	None:intergenic	30.0%
!!	TGTTAGCTTACTTTCTCTTT+TGG	-	chr8.4:31688447-31688466	MS.gene23852:intron	30.0%
!!!	CCACATGTCATTTTTTTATG+TGG	+	chr8.4:31690596-31690615	None:intergenic	30.0%
!!!	TCATTTTTTTATGTGGGTGT+AGG	+	chr8.4:31690589-31690608	None:intergenic	30.0%
	ACAAATTACCATCTCCTTGT+TGG	-	chr8.4:31690718-31690737	MS.gene23852:CDS	35.0%
	ACTAACCTGATAAATTCCAC+CGG	+	chr8.4:31689158-31689177	None:intergenic	35.0%
	AGAGACAATTAGTGAAGTGT+GGG	+	chr8.4:31690629-31690648	None:intergenic	35.0%
	AGGCGACAAGAACTATATAA+AGG	+	chr8.4:31688728-31688747	None:intergenic	35.0%
	ATGGATGCAGCAATATGTAA+AGG	+	chr8.4:31688209-31688228	None:intergenic	35.0%
	CAAAAAAAATACGCGAAGAG+GGG	-	chr8.4:31688574-31688593	MS.gene23852:intron	35.0%
	CAAATTAAGGTTATGGTCAC+CGG	-	chr8.4:31689760-31689779	MS.gene23852:CDS	35.0%
	CATCTTGAATTACTGTGTAG+AGG	+	chr8.4:31688139-31688158	None:intergenic	35.0%
	CATTACAACAGACATATCAG+AGG	-	chr8.4:31689060-31689079	MS.gene23852:intron	35.0%
	CTATTTGTCACAATTCAAGG+AGG	+	chr8.4:31690093-31690112	None:intergenic	35.0%
	CTCAAAAAAAATACGCGAAG+AGG	-	chr8.4:31688572-31688591	MS.gene23852:intron	35.0%
	CTCTCCATTATTTGTGTTTG+TGG	+	chr8.4:31688078-31688097	None:intergenic	35.0%
	GAAAAAGGAAAAGAGAAGCT+AGG	+	chr8.4:31690672-31690691	None:intergenic	35.0%
	GAAACAATGCTTAGCAAACA+TGG	+	chr8.4:31689723-31689742	None:intergenic	35.0%
	GACAAATAGTGCATTGTGAT+TGG	-	chr8.4:31690104-31690123	MS.gene23852:intron	35.0%
	GATCAAATGTGGGATAATCA+GGG	+	chr8.4:31688985-31689004	None:intergenic	35.0%
	GCACTATTTGTCACAATTCA+AGG	+	chr8.4:31690096-31690115	None:intergenic	35.0%
	GGAAAACGAAAGTAAGAATG+AGG	+	chr8.4:31690812-31690831	None:intergenic	35.0%
	GGTAGAAATGGTAAAATTGG+AGG	+	chr8.4:31689209-31689228	None:intergenic	35.0%
	TCTATACGCTCCAAAATGTA+TGG	+	chr8.4:31688164-31688183	None:intergenic	35.0%
	TGATCAAATGTGGGATAATC+AGG	+	chr8.4:31688986-31689005	None:intergenic	35.0%
	TGGGGTAGAAATGGTAAAAT+TGG	+	chr8.4:31689212-31689231	None:intergenic	35.0%
	TTACATATTGCTGCATCCAT+GGG	-	chr8.4:31688209-31688228	MS.gene23852:CDS	35.0%
	TTAGAGTTAAAGGCAGAGAA+GGG	-	chr8.4:31688381-31688400	MS.gene23852:intron	35.0%
	TTATGCAAGTGAAATTGGTG+AGG	-	chr8.4:31688418-31688437	MS.gene23852:intron	35.0%
	TTTACATATTGCTGCATCCA+TGG	-	chr8.4:31688208-31688227	MS.gene23852:CDS	35.0%
!	AATTTTACCATTTCTACCCC+AGG	-	chr8.4:31689211-31689230	MS.gene23852:intron	35.0%
!	ACTCAATGCTGATTGATAGA+TGG	+	chr8.4:31689131-31689150	None:intergenic	35.0%
!	AGTTGAGTTGTCATGTGAAA+TGG	-	chr8.4:31690503-31690522	MS.gene23852:CDS	35.0%
!	CATTGAATCACTGTTTGCTA+AGG	+	chr8.4:31689928-31689947	None:intergenic	35.0%
!	GTCAAGTATTCAATGGATTC+TGG	+	chr8.4:31688476-31688495	None:intergenic	35.0%
!	TAGTGCTGAAATGTGCAAAA+TGG	+	chr8.4:31688651-31688670	None:intergenic	35.0%
!	TGTTTTCAATTGTGGTGGTT+GGG	+	chr8.4:31690701-31690720	None:intergenic	35.0%
!	TTGTTTTCAATTGTGGTGGT+TGG	+	chr8.4:31690702-31690721	None:intergenic	35.0%
!	TTTCCCTATGTTTTCTGTTG+CGG	-	chr8.4:31690827-31690846	MS.gene23852:CDS	35.0%
!!	ATATATAAGTTGGTAGGGGA+GGG	+	chr8.4:31688752-31688771	None:intergenic	35.0%
!!	TATATATAAGTTGGTAGGGG+AGG	+	chr8.4:31688753-31688772	None:intergenic	35.0%
	AAAAGAGAAGCTAGGGATGA+AGG	+	chr8.4:31690664-31690683	None:intergenic	40.0%
	AACACAGGCAGTTCTATTGT+TGG	-	chr8.4:31688838-31688857	MS.gene23852:intron	40.0%
	AACATTTCCTGGGGTAGAAA+TGG	+	chr8.4:31689221-31689240	None:intergenic	40.0%
	AAGCTAGGGATGAAGGAAAA+AGG	+	chr8.4:31690657-31690676	None:intergenic	40.0%
	AATTGTGGTGGTTGGGATAA+AGG	+	chr8.4:31690694-31690713	None:intergenic	40.0%
	ACACAGGCAGTTCTATTGTT+GGG	-	chr8.4:31688839-31688858	MS.gene23852:intron	40.0%
	AGCGATACACCATAAGTTCT+TGG	+	chr8.4:31688337-31688356	None:intergenic	40.0%
	AGGCATGTACTTCAACGATA+TGG	+	chr8.4:31690254-31690273	None:intergenic	40.0%
	ATCACAGAGTTGACATGTGT+AGG	+	chr8.4:31689424-31689443	None:intergenic	40.0%
	ATCCCGCAACAGAAAACATA+GGG	+	chr8.4:31690833-31690852	None:intergenic	40.0%
	ATTTCTACCCCAGGAAATGT+TGG	-	chr8.4:31689220-31689239	MS.gene23852:intron	40.0%
	CCATTGAATACTTGACAGTG+AGG	-	chr8.4:31688480-31688499	MS.gene23852:intron	40.0%
	CCTCACTGTCAAGTATTCAA+TGG	+	chr8.4:31688483-31688502	None:intergenic	40.0%
	CTAACCTGATAAATTCCACC+GGG	+	chr8.4:31689157-31689176	None:intergenic	40.0%
	CTATGGTGGTATACCTATCA+AGG	+	chr8.4:31690552-31690571	None:intergenic	40.0%
	CTCTGCCTTTAACTCTAACA+AGG	+	chr8.4:31688379-31688398	None:intergenic	40.0%
	CTGATTTCCCAACATTTCCT+GGG	+	chr8.4:31689231-31689250	None:intergenic	40.0%
	CTTGATAGGTATACCACCAT+AGG	-	chr8.4:31690550-31690569	MS.gene23852:CDS	40.0%
	GAAAGTAAGAATGAGGGAGA+TGG	+	chr8.4:31690805-31690824	None:intergenic	40.0%
	GACGCTGAGATCGATATTAT+TGG	+	chr8.4:31689861-31689880	None:intergenic	40.0%
	GAGACAATTAGTGAAGTGTG+GGG	+	chr8.4:31690628-31690647	None:intergenic	40.0%
	GAGAGACAATTAGTGAAGTG+TGG	+	chr8.4:31690630-31690649	None:intergenic	40.0%
	GTGGTTGGGATAAAGGAAAA+AGG	+	chr8.4:31690687-31690706	None:intergenic	40.0%
	GTTAGAGTTAAAGGCAGAGA+AGG	-	chr8.4:31688380-31688399	MS.gene23852:intron	40.0%
	TAACCTGATAAATTCCACCG+GGG	+	chr8.4:31689156-31689175	None:intergenic	40.0%
	TATCCCGCAACAGAAAACAT+AGG	+	chr8.4:31690834-31690853	None:intergenic	40.0%
	TCACAGAGTTGACATGTGTA+GGG	+	chr8.4:31689423-31689442	None:intergenic	40.0%
	TGATTTCCCAACATTTCCTG+GGG	+	chr8.4:31689230-31689249	None:intergenic	40.0%
	TGTGGCTCAAAAATAGGAAG+TGG	+	chr8.4:31690070-31690089	None:intergenic	40.0%
	TTAAACAGAAGGGATAAGGC+AGG	-	chr8.4:31688620-31688639	MS.gene23852:intron	40.0%
	TTTCTACCCCAGGAAATGTT+GGG	-	chr8.4:31689221-31689240	MS.gene23852:intron	40.0%
	TTTGCAACTCAAACCACTTC+TGG	-	chr8.4:31689823-31689842	MS.gene23852:CDS	40.0%
!	ACTGATTTCCCAACATTTCC+TGG	+	chr8.4:31689232-31689251	None:intergenic	40.0%
!	ACTTTTCTCTCCTTCATCCA+TGG	-	chr8.4:31689694-31689713	MS.gene23852:CDS	40.0%
!	AGTGAAGTATCCCACTAATG+GGG	+	chr8.4:31689632-31689651	None:intergenic	40.0%
!	ATTTTGCATTGCGAGATGGA+TGG	+	chr8.4:31688312-31688331	None:intergenic	40.0%
!	CTCTTCAAGTACTTGTTAGC+TGG	-	chr8.4:31688549-31688568	MS.gene23852:intron	40.0%
!	GAGTGAAGTATCCCACTAAT+GGG	+	chr8.4:31689633-31689652	None:intergenic	40.0%
!	TGAGTGAAGTATCCCACTAA+TGG	+	chr8.4:31689634-31689653	None:intergenic	40.0%
!	TGTTGGGAAATCAGTCTTGT+CGG	-	chr8.4:31689237-31689256	MS.gene23852:intron	40.0%
!	TTCCCTATGTTTTCTGTTGC+GGG	-	chr8.4:31690828-31690847	MS.gene23852:CDS	40.0%
!	TTTTGCATTGCGAGATGGAT+GGG	+	chr8.4:31688311-31688330	None:intergenic	40.0%
!	TTTTTATGTGGGTGTAGGAG+TGG	+	chr8.4:31690584-31690603	None:intergenic	40.0%
!!	AGAAGTGGTTTGAGTTGCAA+AGG	+	chr8.4:31689824-31689843	None:intergenic	40.0%
!!	GGTTATTTTGCATTGCGAGA+TGG	+	chr8.4:31688316-31688335	None:intergenic	40.0%
!!	GTTTTGAATTCGACCAGAAG+TGG	+	chr8.4:31689839-31689858	None:intergenic	40.0%
!!	TATATAAGTTGGTAGGGGAG+GGG	+	chr8.4:31688751-31688770	None:intergenic	40.0%
	AACATGGCATCCATGGATGA+AGG	+	chr8.4:31689707-31689726	None:intergenic	45.0%
	AACCTGATAAATTCCACCGG+GGG	+	chr8.4:31689155-31689174	None:intergenic	45.0%
	AGTTGTAACCACCCCATTAG+TGG	-	chr8.4:31689618-31689637	MS.gene23852:intron	45.0%
	ATGGAGTCCGATTCAACAAG+AGG	+	chr8.4:31690786-31690805	None:intergenic	45.0%
	ATGGTGATTGACGTCATTGC+TGG	-	chr8.4:31689478-31689497	MS.gene23852:intron	45.0%
	ATTACCATCTCCTTGTTGGC+TGG	-	chr8.4:31690722-31690741	MS.gene23852:CDS	45.0%
	CAACCACCCTCTTGTTGAAT+CGG	-	chr8.4:31690776-31690795	MS.gene23852:CDS	45.0%
	CACAGAGTTGACATGTGTAG+GGG	+	chr8.4:31689422-31689441	None:intergenic	45.0%
	CGTGCCATCATCTATATGAC+CGG	+	chr8.4:31689782-31689801	None:intergenic	45.0%
	CTTAGCAAACATGGCATCCA+TGG	+	chr8.4:31689714-31689733	None:intergenic	45.0%
	GATAGGTATACCACCATAGG+TGG	-	chr8.4:31690553-31690572	MS.gene23852:CDS	45.0%
	GGCAAAGTCAATATGACCCA+TGG	+	chr8.4:31688228-31688247	None:intergenic	45.0%
	GTCACCGGTCATATAGATGA+TGG	-	chr8.4:31689775-31689794	MS.gene23852:CDS	45.0%
	GTTGTAACCACCCCATTAGT+GGG	-	chr8.4:31689619-31689638	MS.gene23852:intron	45.0%
	TAATCAGGGACTGCTAAGCT+AGG	+	chr8.4:31688971-31688990	None:intergenic	45.0%
	TCATTGCTGGATTGTACGCA+AGG	-	chr8.4:31689491-31689510	MS.gene23852:intron	45.0%
	TCTCTAGTGGCTTCTCCAAT+CGG	-	chr8.4:31689355-31689374	MS.gene23852:intron	45.0%
	TGGAGTCCGATTCAACAAGA+GGG	+	chr8.4:31690785-31690804	None:intergenic	45.0%
	TGTCACAATTCAAGGAGGTG+TGG	+	chr8.4:31690088-31690107	None:intergenic	45.0%
	TTTGCATTGCGAGATGGATG+GGG	+	chr8.4:31688310-31688329	None:intergenic	45.0%
!	AGGAGGTGTGGCTCAAAAAT+AGG	+	chr8.4:31690076-31690095	None:intergenic	45.0%
!	GTCATATAGATGATGGCACG+AGG	-	chr8.4:31689782-31689801	MS.gene23852:CDS	45.0%
!	GTTGGGAAATCAGTCTTGTC+GGG	-	chr8.4:31689238-31689257	MS.gene23852:intron	45.0%
!	TTGGGAAATCAGTCTTGTCG+GGG	-	chr8.4:31689239-31689258	MS.gene23852:intron	45.0%
!!	GCTTTGAAGCTAAATCCGCA+AGG	-	chr8.4:31688278-31688297	MS.gene23852:intron	45.0%
	ACCTGATAAATTCCACCGGG+GGG	+	chr8.4:31689154-31689173	None:intergenic	50.0%
	AGCACTAAGTAGTGACCCAC+AGG	-	chr8.4:31688664-31688683	MS.gene23852:intron	50.0%
	AGGAGTGGTATTGCCACCTA+TGG	+	chr8.4:31690569-31690588	None:intergenic	50.0%
	AGTCCGATTCAACAAGAGGG+TGG	+	chr8.4:31690782-31690801	None:intergenic	50.0%
	AGTGGTATTGCCACCTATGG+TGG	+	chr8.4:31690566-31690585	None:intergenic	50.0%
	CAGAGGAGCAACGTAACACT+TGG	-	chr8.4:31689077-31689096	MS.gene23852:intron	50.0%
	TCAACAAGAGGGTGGTTGCA+TGG	+	chr8.4:31690774-31690793	None:intergenic	50.0%
	TTGAAAGCTGCAGCTGAAGC+AGG	-	chr8.4:31688101-31688120	MS.gene23852:CDS	50.0%
!	GAAGTATCCCACTAATGGGG+TGG	+	chr8.4:31689629-31689648	None:intergenic	50.0%
	AGAGGGTGGTTGCATGGTTG+TGG	+	chr8.4:31690768-31690787	None:intergenic	55.0%
	CTAGCGGTAAACCAGCCGAT+TGG	+	chr8.4:31689373-31689392	None:intergenic	55.0%
	GCGAAGAGGGGCTAAAGATC+TGG	-	chr8.4:31688586-31688605	MS.gene23852:intron	55.0%
	TAGTGGCTTCTCCAATCGGC+TGG	-	chr8.4:31689359-31689378	MS.gene23852:intron	55.0%
	TCCCCCCGGTGGAATTTATC+AGG	-	chr8.4:31689150-31689169	MS.gene23852:intron	55.0%
	TGGATGGGGCTGAATCCTTG+CGG	+	chr8.4:31688296-31688315	None:intergenic	55.0%
!	CAATCAGCATTGAGTCCCCC+CGG	-	chr8.4:31689136-31689155	MS.gene23852:intron	55.0%
!!	ATAAGTTGGTAGGGGAGGGG+AGG	+	chr8.4:31688748-31688767	None:intergenic	55.0%
	GGGTGGTTGCATGGTTGTGG+CGG	+	chr8.4:31690765-31690784	None:intergenic	60.0%
	CATGGTTGTGGCGGTGCCTG+AGG	+	chr8.4:31690756-31690775	None:intergenic	65.0%
	GCGGCCAGCCAACAAGGAGA+TGG	+	chr8.4:31690729-31690748	None:intergenic	65.0%
!	TCAGCATTGAGTCCCCCCGG+TGG	-	chr8.4:31689139-31689158	MS.gene23852:intron	65.0%
	GGAGCTGCGGCCAGCCAACA+AGG	+	chr8.4:31690735-31690754	None:intergenic	70.0%
	TGGCGGTGCCTGAGGAGCTG+CGG	+	chr8.4:31690748-31690767	None:intergenic	70.0%
!	TTGGCTGGCCGCAGCTCCTC+AGG	-	chr8.4:31690737-31690756	MS.gene23852:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.4	gene	31688068	31690875	31688068	ID=MS.gene23852
chr8.4	mRNA	31688068	31690875	31688068	ID=MS.gene23852.t1;Parent=MS.gene23852
chr8.4	exon	31690258	31690875	31690258	ID=MS.gene23852.t1.exon1;Parent=MS.gene23852.t1
chr8.4	CDS	31690258	31690875	31690258	ID=cds.MS.gene23852.t1;Parent=MS.gene23852.t1
chr8.4	exon	31689683	31690098	31689683	ID=MS.gene23852.t1.exon2;Parent=MS.gene23852.t1
chr8.4	CDS	31689683	31690098	31689683	ID=cds.MS.gene23852.t1;Parent=MS.gene23852.t1
chr8.4	exon	31688068	31688269	31688068	ID=MS.gene23852.t1.exon3;Parent=MS.gene23852.t1
chr8.4	CDS	31688068	31688269	31688068	ID=cds.MS.gene23852.t1;Parent=MS.gene23852.t1

Gene Sequence

>MS.gene23852

ATGAACACCACAAACACAAATAATGGAGAGCGATTGAAAGCTGCAGCTGAAGCAGGTGATATAGATATCCTCTACACAGTAATTCAAGATGATCCATACATTTTGGAGCGTATAGATTCAATACCATTTGTTGAAACTCCTTTACATATTGCTGCATCCATGGGTCATATTGACTTTGCCATTGAAATTATGAATTTGAAACCTTCATTTGCTTTGAAGCTAAATCCGCAAGGATTCAGCCCCATCCATCTCGCAATGCAAAATAACCAAGAACTTATGGTGTATCGCTTTGTGTTTATCAATAAAGACCTTGTTAGAGTTAAAGGCAGAGAAGGGATAACTCCTCTTCATTATGCAAGTGAAATTGGTGAGGTTAAAATGTTAGCTTACTTTCTCTTTTGGTGTCCAGAATCCATTGAATACTTGACAGTGAGGCGCGAGACTGCACTCCATATTGCTGTTAAGAATGAACAATATGAGGCTCTTCAAGTACTTGTTAGCTGGCTCAAAAAAAATACGCGAAGAGGGGCTAAAGATCTGGAAAATAAAATATTAAACAGAAGGGATAAGGCAGGCAATACCATTTTGCACATTTCAGCACTAAGTAGTGACCCACAGGTAATCTTAATCTTATGCCATTACTAAAATCACATGTCTCCTTTATATAGTTCTTGTCGCCTCCCCTCCCCTACCAACTTATATATAAATAAGATATAGAATTGCTAGTATGAATGATCTCTAACAATTGTATTATTCATTAATTTCTTGGTAACACAGGCAGTTCTATTGTTGGGAAGTACTAGTATAGATTTGAAGGCAAAGAATATGGAGAACAAAACTGCATTAGACATAGCAAGCACACCAGAGATCAAGAGTATATTATTAAGCGTTGGAGCAAAACCTAGCTTAGCAGTCCCTGATTATCCCACATTTGATCATAGGATAAGATCAAAGATCACAACTATAGGTGCTCTGACAATTTATACAGATCGCATTACAACAGACATATCAGAGGAGCAACGTAACACTTGGTTGATAGTTGCGACTCTTGTTGCAACTGCCATCTATCAATCAGCATTGAGTCCCCCCGGTGGAATTTATCAGGTTAGTGCTAGTGACACTAACGTGAACATCACTTCCTCCAATTTTACCATTTCTACCCCAGGAAATGTTGGGAAATCAGTCTTGTCGGGGTATGAATTCTTTTTGTTTTTATTTGTTAATATGTATTCCTTTTCTGTATCAATTTTAGCTATATTTTTTATGATACCATATGGAATAATATTCTCTCTAGTGGCTTCTCCAATCGGCTGGTTTACCGCTAGTTATTTATTTTCTATGTGGAGGATATCCCCTACACATGTCAACTCTGTGATTTTATTCATCATTTTTAGTTCATTTCTGTTATACATGGTGATTGACGTCATTGCTGGATTGTACGCAAGGCTTAAACACAGAATTGCTAAGATATTTAATATGATAGTCAAAGCTATTAGAGATATAGTTTAGTTATAAGTAGTAGTATTAGTTCTATATATAGTAGTATATATAGTTGTAACCACCCCATTAGTGGGATACTTCACTCATCTGTAATATTCCATTTACATCAATACAAAGAGTTTGTTAACTTTTCTCTCCTTCATCCATGGATGCCATGTTTGCTAAGCATTGTTTCAATAGGAGAAACATCAACAAATTAAGGTTATGGTCACCGGTCATATAGATGATGGCACGAGGATGATGACTACTAAGACCTTTGCAACTCAAACCACTTCTGGTCGAATTCAAAACCAATAATATCGATCTCAGCGTCGATTTGTAATTTTACTTGTCATTGTTATCTTTGTTTCTGTTCAACCTTAGCAAACAGTGATTCAATGTATTAAATATTTAATCATATCATGTATTTTATTGAAGGAAAATGCTACTTCTCTAGAAAAATAGCATCAAGAATTTTTCAAGACACAGTTGACAAATTTTAATTGGATGATTTATTTGTCCACTTCCTATTTTTGAGCCACACCTCCTTGAATTGTGACAAATAGTGCATTGTGATTGGCTACTTTTTATTCTTGAAGAATTCTTGATGTTATTTTTCTAGAGATATAACATTGCTCTTTATTGAATGCATGTGTATTCTATTAATTATTAATTATTGTTTTGAAGAAAATTCATTACTAATGCCATATCGTTGAAGTACATGCCTCACATAATAGCATTATCATGTATAATTATAAGTGTATATGGTTTTGAGATATGTTGTTTATTGCATCGTTCAGATTGTGCGTTTAAATTATCGTATTATCATACTCTGCTCATTTTATTGTTATGAGTATGATAACTCACATTAGTTTGTTTTTTTTTTTTGAACGAAACTCACATTAGTTTGTTTATCGGTATGTTTATTTGCACATCGTTTGTACATCTACATGATGAGTTGAGTTGTCATGTGAAATGGAATCAAAGCAAAATCTAAAGAGTCCTTGATAGGTATACCACCATAGGTGGCAATACCACTCCTACACCCACATAAAAAAATGACATGTGGCAATATGGGCCCCACACTTCACTAATTGTCTCTCTTTTCCTTTTTCCTTCATCCCTAGCTTCTCTTTTCCTTTTTCCTTTATCCCAACCACCACAATTGAAAACAAATTACCATCTCCTTGTTGGCTGGCCGCAGCTCCTCAGGCACCGCCACAACCATGCAACCACCCTCTTGTTGAATCGGACTCCATCTCCCTCATTCTTACTTTCGTTTTCCCTATGTTTTCTGTTGCGGGATACCCCAGCCGCTGCCGACCATGA

Protein sequence

>MS.gene23852.t1

MNTTNTNNGERLKAAAEAGDIDILYTVIQDDPYILERIDSIPFVETPLHIAASMGHIDFAIEIMNLKPSFALKLNPQGFSPIHLAMQNNQELMVYRFVFINKDLVRVKGREGITPLHYASEIGEVKMLAYFLFWCPESIEYLTVRRETALHIAVKNEQYEALQVLVSWLKKNTRRGAKDLENKILNRRDKAGNTILHISALSSDPQAVLLLGSTSIDLKAKNMENKTALDIASTPEIKSILLSVGAKPSLAVPDYPTFDHRIRSKITTIGALTIYTDRITTDISEEQRNTWLIVATLVATAIYQSALSPPGGIYQVSASDTNVNITSSNFTISTPGNVGKSVLSGFSFPFSFIPTTTIENKLPSPCWLAAAPQAPPQPCNHPLVESDSISLILTFVFPMFSVAGYPSRCRP