AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene89986

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene89986.t1 MTR_7g451420 70.000 310 88 3 8 317 8 312 1.28e-149 425
MS.gene89986.t1 MTR_8g066670 62.609 345 118 4 1 339 1067 1406 6.51e-134 417
MS.gene89986.t1 MTR_8g467780 59.429 350 128 4 3 339 5 353 1.74e-133 389
MS.gene89986.t1 MTR_8g072510 59.882 339 123 5 8 339 9 341 5.04e-132 385
MS.gene89986.t1 MTR_8g039620 59.706 340 125 4 8 339 13 348 2.39e-131 383
MS.gene89986.t1 MTR_8g073260 58.754 337 130 4 7 339 9 340 1.58e-128 376
MS.gene89986.t1 MTR_3g016210 57.101 345 126 5 14 339 15 356 4.27e-128 375
MS.gene89986.t1 MTR_7g021090 56.358 346 133 4 1 339 1 335 3.30e-124 364
MS.gene89986.t1 MTR_1g059930 55.932 354 141 5 1 344 1 349 8.30e-123 361
MS.gene89986.t1 MTR_8g062410 59.412 340 118 6 8 339 9 336 1.61e-121 358
MS.gene89986.t1 MTR_8g069880 59.050 337 127 4 9 339 7 338 1.20e-119 354
MS.gene89986.t1 MTR_8g068990 54.023 348 135 5 1 339 1 332 8.38e-118 349
MS.gene89986.t1 MTR_8g040930 56.229 297 122 3 52 342 2 296 1.59e-108 323
MS.gene89986.t1 MTR_8g041240 56.229 297 122 3 52 342 2 296 1.59e-108 323
MS.gene89986.t1 MTR_3g105650 45.246 305 154 4 17 314 13 311 2.83e-88 273
MS.gene89986.t1 MTR_8g073275 42.857 364 137 9 52 355 2 354 9.01e-79 249
MS.gene89986.t1 MTR_1g069110 26.974 304 194 9 18 317 27 306 2.63e-23 100
MS.gene89986.t1 MTR_7g099580 28.431 306 163 10 17 317 20 274 4.83e-21 93.2
MS.gene89986.t1 MTR_7g099570 30.481 187 116 3 18 199 18 195 2.24e-17 84.0
MS.gene89986.t1 MTR_2g101320 27.500 240 145 6 40 263 137 363 1.19e-15 78.6
MS.gene89986.t1 MTR_1g115575 25.413 303 183 8 44 325 169 449 5.51e-13 70.5
MS.gene89986.t1 MTR_7g117620 27.468 233 149 4 44 273 119 334 1.91e-12 68.9
MS.gene89986.t1 MTR_4g062100 24.893 233 157 3 44 273 108 325 1.99e-12 68.6
MS.gene89986.t1 MTR_2g101310 27.979 193 126 4 40 232 137 316 3.20e-12 68.2
MS.gene89986.t1 MTR_2g101310 27.979 193 126 4 40 232 137 316 6.16e-12 67.0
MS.gene89986.t1 MTR_1g032100 26.606 218 136 6 44 253 94 295 2.21e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene89986.t1 AT4G10720 38.889 342 188 5 1 332 1 331 8.04e-76 240
MS.gene89986.t1 AT4G10720 38.571 350 194 5 1 340 1 339 6.19e-75 239
MS.gene89986.t1 AT5G54620 41.100 309 171 4 9 311 6 309 1.06e-67 219
MS.gene89986.t1 AT5G54610 39.355 310 170 5 8 308 4 304 4.16e-63 207
MS.gene89986.t1 AT5G15500 39.228 311 168 7 17 315 14 315 1.06e-61 205
MS.gene89986.t1 AT1G14480 36.176 340 186 7 8 329 4 330 7.24e-61 202
MS.gene89986.t1 AT1G14500 35.294 323 195 4 8 321 4 321 1.72e-58 196
MS.gene89986.t1 AT1G14480 34.985 323 170 6 8 329 4 287 1.11e-48 169
MS.gene89986.t1 AT5G51160 28.148 270 163 5 71 320 43 301 3.18e-27 112
MS.gene89986.t1 AT5G15500 33.032 221 121 9 110 315 1 209 6.65e-22 95.9
MS.gene89986.t1 AT2G01680 29.167 216 129 6 44 251 97 296 3.24e-15 77.4
MS.gene89986.t1 AT4G03500 26.923 312 162 12 43 314 217 502 8.38e-15 76.3
MS.gene89986.t1 AT4G03500 26.923 312 162 12 43 314 194 479 8.90e-15 76.3
MS.gene89986.t1 AT4G03500 26.923 312 162 12 43 314 194 479 8.90e-15 76.3
MS.gene89986.t1 AT4G03460 31.278 227 114 9 100 314 258 454 1.77e-14 75.1
MS.gene89986.t1 AT4G03460 31.278 227 114 9 100 314 290 486 1.94e-14 75.1
MS.gene89986.t1 AT5G50140 24.444 270 172 7 38 288 24 280 2.08e-12 68.6
MS.gene89986.t1 AT5G54710 28.302 265 150 9 79 317 246 496 4.61e-12 67.8
MS.gene89986.t1 AT5G54710 28.302 265 150 9 79 317 201 451 4.98e-12 67.8
MS.gene89986.t1 AT5G02620 22.807 342 210 10 19 332 70 385 1.43e-11 66.2
MS.gene89986.t1 AT5G02620 22.807 342 210 10 19 332 70 385 1.43e-11 66.2
MS.gene89986.t1 AT5G02620 22.807 342 210 10 19 332 70 385 1.43e-11 66.2

Find 81 sgRNAs with CRISPR-Local

Find 133 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAGACCTTGTTAGAATTAA+AGG 0.138850 4.2:+48138049 MS.gene89986:CDS
TAAGGATGATCCATCCATTT+TGG 0.181943 4.2:+48137829 MS.gene89986:CDS
TAGAATATAAAATATTAAAT+TGG 0.253258 4.2:+48138284 MS.gene89986:CDS
CGAATCTATATTATCCAAAA+TGG 0.262933 4.2:-48137843 None:intergenic
CTTCATATTGCAAGTCAAAT+TGG 0.274428 4.2:+48138091 MS.gene89986:CDS
CACCGGGTCCAGATAGATTA+TGG 0.294634 4.2:+48139434 MS.gene89986:CDS
CATCCTCTACACAGTAATTA+AGG 0.299377 4.2:+48137811 MS.gene89986:CDS
AGCGAAGCGCCATCCTGTTT+TGG 0.323705 4.2:-48138012 None:intergenic
TTCAGTTTATAAATTAGATT+GGG 0.335488 4.2:-48137761 None:intergenic
CGATCAAACCATAATCTATC+TGG 0.337274 4.2:-48139442 None:intergenic
TGACGGCCAATGTAGATATT+AGG 0.348577 4.2:+48138768 MS.gene89986:CDS
TTTCAGTTTATAAATTAGAT+TGG 0.351179 4.2:-48137762 None:intergenic
TAAATTCCTCATTGCTTGTC+AGG 0.384221 4.2:+48138132 MS.gene89986:CDS
ACTGATTTACCAGTATTTCC+TGG 0.384646 4.2:-48139064 None:intergenic
CTTTGCATATCGCCGCATCT+AGG 0.387295 4.2:+48137885 MS.gene89986:CDS
TTGTGCTAAAATGTGCAAAA+TGG 0.415943 4.2:-48138326 None:intergenic
AAAGACAAGGATAAATTTGA+AGG 0.432984 4.2:+48138712 MS.gene89986:CDS
TTTGCATATCGCCGCATCTA+GGG 0.439653 4.2:+48137886 MS.gene89986:CDS
CCGGTGACCATAACCTTGAT+TGG 0.450763 4.2:-48139417 None:intergenic
AGAAACACCAATCAAGGTTA+TGG 0.451091 4.2:+48139410 MS.gene89986:CDS
TGCAAAGGAGTTTCAACAAA+CGG 0.452128 4.2:-48137869 None:intergenic
TTTCAAATTCATAACCTCAA+TGG 0.456133 4.2:-48137924 None:intergenic
CTATGGAAAACAGCCAAAAC+AGG 0.465031 4.2:+48137999 MS.gene89986:CDS
TTCTAGGTAGCACAGGCGCT+TGG 0.467704 4.2:+48138680 MS.gene89986:intron
CTTAGTTTATATGCAAGTGT+TGG 0.472326 4.2:-48138842 None:intergenic
GCATATCCGGTTTGCCATTG+AGG 0.477173 4.2:+48137910 MS.gene89986:CDS
TCATAACCTCAATGGCAAAC+CGG 0.477798 4.2:-48137916 None:intergenic
CCTCGCAGTCACATCTTCAA+TGG 0.482300 4.2:-48138158 None:intergenic
CCCTTCCTTTAATTCTAACA+AGG 0.482377 4.2:-48138054 None:intergenic
ATCGTTACTCCAGGAAATAC+TGG 0.485721 4.2:+48139055 MS.gene89986:CDS
CCAATCAAGGTTATGGTCAC+CGG 0.490176 4.2:+48139417 MS.gene89986:CDS
TTGGAGCATCGGTGACTTGT+TGG 0.497457 4.2:-48138823 None:intergenic
GAGCCCTCTCCACCTTGCTA+TGG 0.499528 4.2:+48137982 MS.gene89986:CDS
TTAAGCTTCGAAGCAAATGA+AGG 0.502154 4.2:-48137947 None:intergenic
CAGTTTATTATCTCTAATTG+TGG 0.505449 4.2:-48138873 None:intergenic
AGTTAAATCATTGATGCAAT+CGG 0.506582 4.2:+48139387 MS.gene89986:CDS
GGGACTCAATGATGATTGAT+AGG 0.508072 4.2:-48138984 None:intergenic
AATATTAAATTGGAAGGATG+AGG 0.519869 4.2:+48138294 MS.gene89986:CDS
AGGAGTATATTGTTGAGTGC+AGG 0.521591 4.2:+48138788 MS.gene89986:CDS
GCATCGGTGACTTGTTGGCC+AGG 0.522642 4.2:-48138818 None:intergenic
CTTGTTAGAATTAAAGGAAG+GGG 0.524352 4.2:+48138055 MS.gene89986:CDS
TTGCATATCGCCGCATCTAG+GGG 0.527689 4.2:+48137887 MS.gene89986:CDS
ATTTCATTTCTAGGTAGCAC+AGG 0.528889 4.2:+48138673 MS.gene89986:intron
ATATGCAAGTGTTGGAGCAT+CGG 0.532301 4.2:-48138834 None:intergenic
CAATCATCATTGAGTCCCAC+TGG 0.542164 4.2:+48138989 MS.gene89986:CDS
ACCTTGTTAGAATTAAAGGA+AGG 0.545216 4.2:+48138053 MS.gene89986:CDS
AATCGGAGAAACACCAATCA+AGG 0.549153 4.2:+48139404 MS.gene89986:CDS
ATTTGACTTGCAATATGAAG+AGG 0.556104 4.2:-48138088 None:intergenic
TTTCCATAGCAAGGTGGAGA+GGG 0.556707 4.2:-48137985 None:intergenic
TCTATATTATCCAAAATGGA+TGG 0.560946 4.2:-48137839 None:intergenic
GGAGTTTATCAAGTTAGTGC+TGG 0.563187 4.2:+48139013 MS.gene89986:CDS
ATATAAAATATTAAATTGGA+AGG 0.566615 4.2:+48138288 MS.gene89986:CDS
CAGTGGAGCAACGTAACACT+TGG 0.567850 4.2:+48138930 MS.gene89986:CDS
GATGTAGACATCGTTACTCC+AGG 0.569739 4.2:+48139046 MS.gene89986:CDS
GTTAGAATTAAAGGAAGGGG+AGG 0.574654 4.2:+48138058 MS.gene89986:CDS
AAGACAAGGATAAATTTGAA+GGG 0.577076 4.2:+48138713 MS.gene89986:CDS
CAATCAAGGTTATGGTCACC+GGG 0.583109 4.2:+48139418 MS.gene89986:CDS
CCTTGTTAGAATTAAAGGAA+GGG 0.591674 4.2:+48138054 MS.gene89986:CDS
CATCCTTAATTACTGTGTAG+AGG 0.592515 4.2:-48137814 None:intergenic
GCTTCGAAGCTTAATTCGCA+AGG 0.593504 4.2:+48137956 MS.gene89986:CDS
TATACTCCTAATATCTACAT+TGG 0.605385 4.2:-48138774 None:intergenic
CTAGATGCGGCGATATGCAA+AGG 0.606329 4.2:-48137884 None:intergenic
AAAATATCAAGAGAGGTGCT+AGG 0.606950 4.2:+48138257 MS.gene89986:CDS
AACCATAATCTATCTGGACC+CGG 0.610448 4.2:-48139436 None:intergenic
TCATCATTGAGTCCCACTGG+TGG 0.616877 4.2:+48138992 MS.gene89986:CDS
TTGGATTGTTGTTAAAGACA+AGG 0.621765 4.2:+48138699 MS.gene89986:CDS
TCAGTTTATAAATTAGATTG+GGG 0.634387 4.2:-48137760 None:intergenic
GGAAAACAGCCAAAACAGGA+TGG 0.635377 4.2:+48138003 MS.gene89986:CDS
TATTGCAAGTCAAATTGGAG+AGG 0.642645 4.2:+48138096 MS.gene89986:CDS
AACTTGATAAACTCCACCAG+TGG 0.648493 4.2:-48139005 None:intergenic
CATTGAAGATGTGACTGCGA+GGG 0.648859 4.2:+48138159 MS.gene89986:CDS
TAACACTTGGATGATAGTTG+CGG 0.650738 4.2:+48138943 MS.gene89986:CDS
TTGAGTGCAGGTGTAAAACC+TGG 0.654502 4.2:+48138800 MS.gene89986:CDS
ACAAAGGAATTTCGAGCCAG+AGG 0.654770 4.2:+48138345 MS.gene89986:CDS
TGCATATCGCCGCATCTAGG+GGG 0.655530 4.2:+48137888 MS.gene89986:CDS
CCATTGAAGATGTGACTGCG+AGG 0.657318 4.2:+48138158 MS.gene89986:CDS
ACTTGATAAACTCCACCAGT+GGG 0.663884 4.2:-48139004 None:intergenic
TGGAATCCTGACAAGCAATG+AGG 0.669506 4.2:-48138138 None:intergenic
CAAAACAGCATTAGACATGA+CGG 0.677185 4.2:+48138751 MS.gene89986:CDS
ATTGAAGATGTGACTGCGAG+GGG 0.694619 4.2:+48138160 MS.gene89986:CDS
ACTCAATGATGATTGATAGG+TGG 0.718600 4.2:-48138981 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATATCACATAAAAAAATA+GGG + chr4.2:48138530-48138549 MS.gene89986:intron 10.0%
!! ATATAAAATATTAAATTGGA+AGG + chr4.2:48138288-48138307 MS.gene89986:CDS 10.0%
!!! TTAATATATGAATACATTTT+GGG + chr4.2:48138553-48138572 MS.gene89986:intron 10.0%
!! GAAATATCACATAAAAAAAT+AGG + chr4.2:48138529-48138548 MS.gene89986:intron 15.0%
!! TTAGAAAAAATATCTACAAA+AGG - chr4.2:48138374-48138393 None:intergenic 15.0%
!! TTCAGTTTATAAATTAGATT+GGG - chr4.2:48137764-48137783 None:intergenic 15.0%
!! TTTCAGTTTATAAATTAGAT+TGG - chr4.2:48137765-48137784 None:intergenic 15.0%
!!! GTTAATATATGAATACATTT+TGG + chr4.2:48138552-48138571 MS.gene89986:intron 15.0%
!! CAAAAATATGAAAACAACTA+TGG - chr4.2:48139275-48139294 None:intergenic 20.0%
!! TCAGTTTATAAATTAGATTG+GGG - chr4.2:48137763-48137782 None:intergenic 20.0%
! AAAGACAAGGATAAATTTGA+AGG + chr4.2:48138712-48138731 MS.gene89986:CDS 25.0%
! AAAGACCTTGTTAGAATTAA+AGG + chr4.2:48138049-48138068 MS.gene89986:CDS 25.0%
! AAATTTGAAGGGAAAAAACT+TGG + chr4.2:48138724-48138743 MS.gene89986:CDS 25.0%
! AAGACAAGGATAAATTTGAA+GGG + chr4.2:48138713-48138732 MS.gene89986:CDS 25.0%
! AATATTAAATTGGAAGGATG+AGG + chr4.2:48138294-48138313 MS.gene89986:CDS 25.0%
! AATGAAAACATCAAGAAATC+AGG - chr4.2:48139100-48139119 None:intergenic 25.0%
! ACATAGAAAATAAGAACGAA+AGG - chr4.2:48139225-48139244 None:intergenic 25.0%
! AGTTAAATCATTGATGCAAT+CGG + chr4.2:48139387-48139406 MS.gene89986:CDS 25.0%
! ATATTTATTATGATGCCAAG+AGG + chr4.2:48139157-48139176 MS.gene89986:intron 25.0%
! CAGTTTATTATCTCTAATTG+TGG - chr4.2:48138876-48138895 None:intergenic 25.0%
! CATAGAAAATAAGAACGAAA+GGG - chr4.2:48139224-48139243 None:intergenic 25.0%
! CATTGCAAAAAATATAACAG+TGG + chr4.2:48138913-48138932 MS.gene89986:CDS 25.0%
! CGAATCTATATTATCCAAAA+TGG - chr4.2:48137846-48137865 None:intergenic 25.0%
! TATACTCCTAATATCTACAT+TGG - chr4.2:48138777-48138796 None:intergenic 25.0%
! TATTTATTATGATGCCAAGA+GGG + chr4.2:48139158-48139177 MS.gene89986:intron 25.0%
! TCTATATTATCCAAAATGGA+TGG - chr4.2:48137842-48137861 None:intergenic 25.0%
! TTCAAATGATAGTAAAGGTT+CGG - chr4.2:48138504-48138523 None:intergenic 25.0%
! TTCGATTCAAATGATAGTAA+AGG - chr4.2:48138509-48138528 None:intergenic 25.0%
! TTTCAAATTCATAACCTCAA+TGG - chr4.2:48137927-48137946 None:intergenic 25.0%
!!! CATAGTTGATAAAAAAAAGG+AGG - chr4.2:48139134-48139153 None:intergenic 25.0%
!!! CTCCTTTTTTTTATCAACTA+TGG + chr4.2:48139132-48139151 MS.gene89986:intron 25.0%
!!! TGCCATAGTTGATAAAAAAA+AGG - chr4.2:48139137-48139156 None:intergenic 25.0%
!!! TTTCGTTCTTATTTTCTATG+TGG + chr4.2:48139224-48139243 MS.gene89986:intron 25.0%
ACCTTGTTAGAATTAAAGGA+AGG + chr4.2:48138053-48138072 MS.gene89986:CDS 30.0%
CCTTGTTAGAATTAAAGGAA+GGG + chr4.2:48138054-48138073 MS.gene89986:CDS 30.0%
CTTAGTTTATATGCAAGTGT+TGG - chr4.2:48138845-48138864 None:intergenic 30.0%
CTTCATATTGCAAGTCAAAT+TGG + chr4.2:48138091-48138110 MS.gene89986:CDS 30.0%
CTTCGAAAAAATATCAAGAG+AGG + chr4.2:48138250-48138269 MS.gene89986:CDS 30.0%
CTTGTTAGAATTAAAGGAAG+GGG + chr4.2:48138055-48138074 MS.gene89986:CDS 30.0%
TCAAATGATAGTAAAGGTTC+GGG - chr4.2:48138503-48138522 None:intergenic 30.0%
TGTGAAAAATAACAAGTACG+AGG + chr4.2:48138204-48138223 MS.gene89986:CDS 30.0%
TTGTGCTAAAATGTGCAAAA+TGG - chr4.2:48138329-48138348 None:intergenic 30.0%
! TTGGATTGTTGTTAAAGACA+AGG + chr4.2:48138699-48138718 MS.gene89986:CDS 30.0%
!! ATTTGACTTGCAATATGAAG+AGG - chr4.2:48138091-48138110 None:intergenic 30.0%
!! GTCTTGTTGATTTCATTTCT+AGG + chr4.2:48138664-48138683 MS.gene89986:intron 30.0%
!! TTTTGCACATTTTAGCACAA+AGG + chr4.2:48138329-48138348 MS.gene89986:CDS 30.0%
!!! TGCAGATTTTAGTTGACAAT+AGG + chr4.2:48138624-48138643 MS.gene89986:intron 30.0%
ACTCAATGATGATTGATAGG+TGG - chr4.2:48138984-48139003 None:intergenic 35.0%
AGAAACACCAATCAAGGTTA+TGG + chr4.2:48139410-48139429 MS.gene89986:CDS 35.0%
CAAAACAGCATTAGACATGA+CGG + chr4.2:48138751-48138770 MS.gene89986:CDS 35.0%
CATCCTCTACACAGTAATTA+AGG + chr4.2:48137811-48137830 MS.gene89986:CDS 35.0%
CATCCTTAATTACTGTGTAG+AGG - chr4.2:48137817-48137836 None:intergenic 35.0%
CCCTTCCTTTAATTCTAACA+AGG - chr4.2:48138057-48138076 None:intergenic 35.0%
CGATCAAACCATAATCTATC+TGG - chr4.2:48139445-48139464 None:intergenic 35.0%
TAAATTCCTCATTGCTTGTC+AGG + chr4.2:48138132-48138151 MS.gene89986:CDS 35.0%
TAACACTTGGATGATAGTTG+CGG + chr4.2:48138943-48138962 MS.gene89986:CDS 35.0%
TATCTACAAAAGGTTACCTC+TGG - chr4.2:48138364-48138383 None:intergenic 35.0%
TATGAAAACAACTATGGCAC+TGG - chr4.2:48139269-48139288 None:intergenic 35.0%
TATTGCAAGTCAAATTGGAG+AGG + chr4.2:48138096-48138115 MS.gene89986:CDS 35.0%
TGCAAAGGAGTTTCAACAAA+CGG - chr4.2:48137872-48137891 None:intergenic 35.0%
TTAAGCTTCGAAGCAAATGA+AGG - chr4.2:48137950-48137969 None:intergenic 35.0%
! ACTGATTTACCAGTATTTCC+TGG - chr4.2:48139067-48139086 None:intergenic 35.0%
! ATTTCATTTCTAGGTAGCAC+AGG + chr4.2:48138673-48138692 MS.gene89986:intron 35.0%
! TAAGGATGATCCATCCATTT+TGG + chr4.2:48137829-48137848 MS.gene89986:CDS 35.0%
!! AAAATATCAAGAGAGGTGCT+AGG + chr4.2:48138257-48138276 MS.gene89986:CDS 35.0%
AACCATAATCTATCTGGACC+CGG - chr4.2:48139439-48139458 None:intergenic 40.0%
AACTTGATAAACTCCACCAG+TGG - chr4.2:48139008-48139027 None:intergenic 40.0%
AATCGGAGAAACACCAATCA+AGG + chr4.2:48139404-48139423 MS.gene89986:CDS 40.0%
AATGATAGTAAAGGTTCGGG+TGG - chr4.2:48138500-48138519 None:intergenic 40.0%
ACTTGATAAACTCCACCAGT+GGG - chr4.2:48139007-48139026 None:intergenic 40.0%
ATCGTTACTCCAGGAAATAC+TGG + chr4.2:48139055-48139074 MS.gene89986:CDS 40.0%
CTATGGAAAACAGCCAAAAC+AGG + chr4.2:48137999-48138018 MS.gene89986:CDS 40.0%
GGAGTTTATCAAGTTAGTGC+TGG + chr4.2:48139013-48139032 MS.gene89986:CDS 40.0%
GGGACTCAATGATGATTGAT+AGG - chr4.2:48138987-48139006 None:intergenic 40.0%
GTTAGAATTAAAGGAAGGGG+AGG + chr4.2:48138058-48138077 MS.gene89986:CDS 40.0%
TATTATGATGCCAAGAGGGA+TGG + chr4.2:48139162-48139181 MS.gene89986:intron 40.0%
TCATAACCTCAATGGCAAAC+CGG - chr4.2:48137919-48137938 None:intergenic 40.0%
TGACGGCCAATGTAGATATT+AGG + chr4.2:48138768-48138787 MS.gene89986:CDS 40.0%
! AGGAGTATATTGTTGAGTGC+AGG + chr4.2:48138788-48138807 MS.gene89986:CDS 40.0%
!! ATATGCAAGTGTTGGAGCAT+CGG - chr4.2:48138837-48138856 None:intergenic 40.0%
!! TTTGGCTGTTTTCCATAGCA+AGG - chr4.2:48137997-48138016 None:intergenic 40.0%
ACAAAGGAATTTCGAGCCAG+AGG + chr4.2:48138345-48138364 MS.gene89986:CDS 45.0%
ATTGAAGATGTGACTGCGAG+GGG + chr4.2:48138160-48138179 MS.gene89986:CDS 45.0%
CAATCAAGGTTATGGTCACC+GGG + chr4.2:48139418-48139437 MS.gene89986:CDS 45.0%
CAATCATCATTGAGTCCCAC+TGG + chr4.2:48138989-48139008 MS.gene89986:CDS 45.0%
CATTGAAGATGTGACTGCGA+GGG + chr4.2:48138159-48138178 MS.gene89986:CDS 45.0%
CCAATCAAGGTTATGGTCAC+CGG + chr4.2:48139417-48139436 MS.gene89986:CDS 45.0%
GATGTAGACATCGTTACTCC+AGG + chr4.2:48139046-48139065 MS.gene89986:CDS 45.0%
GCTTCGAAGCTTAATTCGCA+AGG + chr4.2:48137956-48137975 MS.gene89986:CDS 45.0%
GGAAAACAGCCAAAACAGGA+TGG + chr4.2:48138003-48138022 MS.gene89986:CDS 45.0%
TGGAATCCTGACAAGCAATG+AGG - chr4.2:48138141-48138160 None:intergenic 45.0%
TTGAGTGCAGGTGTAAAACC+TGG + chr4.2:48138800-48138819 MS.gene89986:CDS 45.0%
TTTCCATAGCAAGGTGGAGA+GGG - chr4.2:48137988-48138007 None:intergenic 45.0%
TTTGCATATCGCCGCATCTA+GGG + chr4.2:48137886-48137905 MS.gene89986:CDS 45.0%
TTTGGGAGAGAGAGAGAGAA+GGG + chr4.2:48138570-48138589 MS.gene89986:intron 45.0%
! CTTTCTAGTGGCTCTTCCAA+TGG + chr4.2:48139189-48139208 MS.gene89986:intron 45.0%
! TTTTCCATAGCAAGGTGGAG+AGG - chr4.2:48137989-48138008 None:intergenic 45.0%
!! TATGGCACTGGTATCTGTAG+AGG - chr4.2:48139257-48139276 None:intergenic 45.0%
!! TTTTGGGAGAGAGAGAGAGA+AGG + chr4.2:48138569-48138588 MS.gene89986:intron 45.0%
!! TAGAATATAAAATATTAAAT+TGG + chr4.2:48138284-48138303 MS.gene89986:CDS 5.0%
ATGATGCCAAGAGGGATGGT+GGG + chr4.2:48139166-48139185 MS.gene89986:intron 50.0%
CACCGGGTCCAGATAGATTA+TGG + chr4.2:48139434-48139453 MS.gene89986:CDS 50.0%
CAGTGGAGCAACGTAACACT+TGG + chr4.2:48138930-48138949 MS.gene89986:CDS 50.0%
CCATTGAAGATGTGACTGCG+AGG + chr4.2:48138158-48138177 MS.gene89986:CDS 50.0%
CCGGTGACCATAACCTTGAT+TGG - chr4.2:48139420-48139439 None:intergenic 50.0%
CCTCGCAGTCACATCTTCAA+TGG - chr4.2:48138161-48138180 None:intergenic 50.0%
CTAGATGCGGCGATATGCAA+AGG - chr4.2:48137887-48137906 None:intergenic 50.0%
CTTTGCATATCGCCGCATCT+AGG + chr4.2:48137885-48137904 MS.gene89986:CDS 50.0%
GAAAGGGCAAACCAAGCCAT+TGG - chr4.2:48139208-48139227 None:intergenic 50.0%
TAGTGGCTCTTCCAATGGCT+TGG + chr4.2:48139194-48139213 MS.gene89986:intron 50.0%
TATGATGCCAAGAGGGATGG+TGG + chr4.2:48139165-48139184 MS.gene89986:intron 50.0%
TCATCATTGAGTCCCACTGG+TGG + chr4.2:48138992-48139011 MS.gene89986:CDS 50.0%
TTGCATATCGCCGCATCTAG+GGG + chr4.2:48137887-48137906 MS.gene89986:CDS 50.0%
TTGGGAGAGAGAGAGAGAAG+GGG + chr4.2:48138571-48138590 MS.gene89986:intron 50.0%
! GCATATCCGGTTTGCCATTG+AGG + chr4.2:48137910-48137929 MS.gene89986:CDS 50.0%
!! GGCTGTTTTCCATAGCAAGG+TGG - chr4.2:48137994-48138013 None:intergenic 50.0%
!! TTGGAGCATCGGTGACTTGT+TGG - chr4.2:48138826-48138845 None:intergenic 50.0%
AGAAAGCCCACCATCCCTCT+TGG - chr4.2:48139175-48139194 None:intergenic 55.0%
TGCATATCGCCGCATCTAGG+GGG + chr4.2:48137888-48137907 MS.gene89986:CDS 55.0%
TGGGAGAGAGAGAGAGAAGG+GGG + chr4.2:48138572-48138591 MS.gene89986:intron 55.0%
TTCTAGGTAGCACAGGCGCT+TGG + chr4.2:48138680-48138699 MS.gene89986:intron 55.0%
!! AGCGAAGCGCCATCCTGTTT+TGG - chr4.2:48138015-48138034 None:intergenic 55.0%
!! AGGGATGGTGGGCTTTCTAG+TGG + chr4.2:48139177-48139196 MS.gene89986:intron 55.0%
CCGGATATGCCCCCTAGATG+CGG - chr4.2:48137900-48137919 None:intergenic 60.0%
GAGCCCTCTCCACCTTGCTA+TGG + chr4.2:48137982-48138001 MS.gene89986:CDS 60.0%
GGAGAGAGAGAGAGAAGGGG+GGG + chr4.2:48138574-48138593 MS.gene89986:intron 60.0%
GGGAGAGAGAGAGAGAAGGG+GGG + chr4.2:48138573-48138592 MS.gene89986:intron 60.0%
! CCGCATCTAGGGGGCATATC+CGG + chr4.2:48137897-48137916 MS.gene89986:CDS 60.0%
!! GCATCGGTGACTTGTTGGCC+AGG - chr4.2:48138821-48138840 None:intergenic 60.0%
AGAGAGAGAGAAGGGGGGGC+GGG + chr4.2:48138578-48138597 MS.gene89986:intron 65.0%
GAGAGAGAGAGAAGGGGGGG+CGG + chr4.2:48138577-48138596 MS.gene89986:intron 65.0%
AGAGAGAGAAGGGGGGGCGG+GGG + chr4.2:48138580-48138599 MS.gene89986:intron 70.0%
GAGAGAGAGAAGGGGGGGCG+GGG + chr4.2:48138579-48138598 MS.gene89986:intron 70.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 48137755 48139468 48137755 ID=MS.gene89986
chr4.2 mRNA 48137755 48139468 48137755 ID=MS.gene89986.t1;Parent=MS.gene89986
chr4.2 exon 48137755 48138366 48137755 ID=MS.gene89986.t1.exon1;Parent=MS.gene89986.t1
chr4.2 CDS 48137755 48138366 48137755 ID=cds.MS.gene89986.t1;Parent=MS.gene89986.t1
chr4.2 exon 48138686 48139084 48138686 ID=MS.gene89986.t1.exon2;Parent=MS.gene89986.t1
chr4.2 CDS 48138686 48139084 48138686 ID=cds.MS.gene89986.t1;Parent=MS.gene89986.t1
chr4.2 exon 48139382 48139468 48139382 ID=MS.gene89986.t1.exon3;Parent=MS.gene89986.t1
chr4.2 CDS 48139382 48139468 48139382 ID=cds.MS.gene89986.t1;Parent=MS.gene89986.t1
Gene Sequence

>MS.gene89986

ATGAACCCCAATCTAATTTATAAACTGAAACTTACAGATGAAGACTATAATATAAACATCCTCTACACAGTAATTAAGGATGATCCATCCATTTTGGATAATATAGATTCGAAACCGTTTGTTGAAACTCCTTTGCATATCGCCGCATCTAGGGGGCATATCCGGTTTGCCATTGAGGTTATGAATTTGAAACCTTCATTTGCTTCGAAGCTTAATTCGCAAGGTTTGAGCCCTCTCCACCTTGCTATGGAAAACAGCCAAAACAGGATGGCGCTTCGCTTTGTTGACATCAACAAAGACCTTGTTAGAATTAAAGGAAGGGGAGGCTTGACTCCTCTTCATATTGCAAGTCAAATTGGAGAGGTCGACATTTTAGCTAAATTCCTCATTGCTTGTCAGGATTCCATTGAAGATGTGACTGCGAGGGGTGAGACTGCTCTACATATTGCTGTGAAAAATAACAAGTACGAGGCTCTTGATCTTCTTGTATGCTTTCTTCGAAAAAATATCAAGAGAGGTGCTAGGAAGTTAGAATATAAAATATTAAATTGGAAGGATGAGGATGACAACACCATTTTGCACATTTTAGCACAAAGGAATTTCGAGCCAGAGGTAACCTTTTGTAGATATTTTTTCTAATGATAAATGTTAATATTTTAATTGTTATGTTAGCTTTTCTTCTTGTCGAAATTTGAACTTTGAATCTTCAATTTCTTAACTCTCAACTCAAACTAGTAGTCAACCACCCGAACCTTTACTATCATTTGAATCGAAGAAATATCACATAAAAAAATAGGGTTAATATATGAATACATTTTGGGAGAGAGAGAGAGAAGGGGGGGCGGGGGTTTGTTTCTTGTACACTACTATGCAGATTTTAGTTGACAATAGGAATGATATCTAACAACTGTCTTGTTGATTTCATTTCTAGGTAGCACAGGCGCTTGGATTGTTGTTAAAGACAAGGATAAATTTGAAGGGAAAAAACTTGGAGAACAAAACAGCATTAGACATGACGGCCAATGTAGATATTAGGAGTATATTGTTGAGTGCAGGTGTAAAACCTGGCCAACAAGTCACCGATGCTCCAACACTTGCATATAAACTAAGATTAAAAACCACAATTAGAGATAATAAACTGAATACTTATCTAAATCGCATTGCAAAAAATATAACAGTGGAGCAACGTAACACTTGGATGATAGTTGCGGCTCTTGTTGCAACTGCCACCTATCAATCATCATTGAGTCCCACTGGTGGAGTTTATCAAGTTAGTGCTGGTGATGACAATGATGTAGACATCGTTACTCCAGGAAATACTGGTAAATCAGTCTTGTCAGAACCTGATTTCTTGATGTTTTCATTTTTAAATCTGTCCTCCTTTTTTTTATCAACTATGGCAATATTTATTATGATGCCAAGAGGGATGGTGGGCTTTCTAGTGGCTCTTCCAATGGCTTGGTTTGCCCTTTCGTTCTTATTTTCTATGTGGAAGATATCCTCTACAGATACCAGTGCCATAGTTGTTTTCATATTTTTGAGTTTAATTCTGTTTTTTGTATTTATTTATAACATTTATTCTGTTTACTCAACATTTAAACGCATGATTGCTAAGATGTTTGAATAGATGATAGTTAAATCATTGATGCAATCGGAGAAACACCAATCAAGGTTATGGTCACCGGGTCCAGATAGATTATGGTTTGATCGCTAA
Protein sequence

>MS.gene89986.t1

MNPNLIYKLKLTDEDYNINILYTVIKDDPSILDNIDSKPFVETPLHIAASRGHIRFAIEVMNLKPSFASKLNSQGLSPLHLAMENSQNRMALRFVDINKDLVRIKGRGGLTPLHIASQIGEVDILAKFLIACQDSIEDVTARGETALHIAVKNNKYEALDLLVCFLRKNIKRGARKLEYKILNWKDEDDNTILHILAQRNFEPEVAQALGLLLKTRINLKGKNLENKTALDMTANVDIRSILLSAGVKPGQQVTDAPTLAYKLRLKTTIRDNKLNTYLNRIAKNITVEQRNTWMIVAALVATATYQSSLSPTGGVYQVSAGDDNDVDIVTPGNTGKSMIVKSLMQSEKHQSRLWSPGPDRLWFDR