AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene067705

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene067705.t1 MTR_8g062410 67.974 459 97 4 4 419 2 453 0.0 578
MS.gene067705.t1 MTR_8g072510 71.566 415 87 5 4 390 2 413 0.0 567
MS.gene067705.t1 MTR_8g039620 63.373 415 117 5 1 382 1 413 6.28e-177 501
MS.gene067705.t1 MTR_7g021090 65.327 398 98 5 1 368 1 388 1.37e-176 499
MS.gene067705.t1 MTR_3g016210 59.551 445 123 6 4 392 2 445 4.69e-169 481
MS.gene067705.t1 MTR_1g059930 58.234 419 168 2 1 419 1 412 3.48e-164 468
MS.gene067705.t1 MTR_8g068990 63.881 371 111 6 1 366 1 353 6.09e-157 451
MS.gene067705.t1 MTR_7g451420 68.506 308 96 1 9 316 6 312 1.07e-145 417
MS.gene067705.t1 MTR_8g073260 53.396 427 155 6 4 390 2 424 7.54e-145 420
MS.gene067705.t1 MTR_8g069880 56.770 421 144 9 12 397 7 424 2.14e-144 419
MS.gene067705.t1 MTR_8g040930 57.029 377 117 7 54 390 1 372 4.15e-138 400
MS.gene067705.t1 MTR_8g041240 57.029 377 117 7 54 390 1 372 4.15e-138 400
MS.gene067705.t1 MTR_8g066670 56.188 404 148 3 9 385 1072 1473 1.06e-134 421
MS.gene067705.t1 MTR_8g467780 51.034 435 164 5 10 397 9 441 9.64e-128 377
MS.gene067705.t1 MTR_3g105650 45.385 390 185 6 10 381 3 382 7.09e-103 312
MS.gene067705.t1 MTR_8g073275 41.667 420 143 8 54 381 1 410 5.52e-87 272
MS.gene067705.t1 MTR_7g099580 23.483 379 231 8 11 383 11 336 4.13e-23 99.8
MS.gene067705.t1 MTR_1g069110 26.300 327 200 8 15 329 21 318 4.93e-23 101
MS.gene067705.t1 MTR_2g101320 28.111 217 139 3 43 252 137 343 5.19e-19 89.7
MS.gene067705.t1 MTR_7g099570 26.728 217 145 3 6 217 3 210 1.23e-18 88.6
MS.gene067705.t1 MTR_2g101310 26.667 255 159 6 39 276 133 376 6.04e-15 77.4
MS.gene067705.t1 MTR_2g101310 26.667 255 159 6 39 276 133 376 1.09e-14 76.3
MS.gene067705.t1 MTR_7g117620 25.087 287 182 7 47 312 119 393 5.53e-14 73.9
MS.gene067705.t1 MTR_1g115575 24.315 292 181 6 47 313 169 445 5.59e-13 71.2
MS.gene067705.t1 MTR_8g105260 24.483 290 183 5 47 312 101 378 5.91e-13 70.9
MS.gene067705.t1 MTR_1g032100 24.221 289 183 5 48 312 95 371 1.21e-12 70.1
MS.gene067705.t1 MTR_2g096190 25.466 161 118 1 7 167 100 258 9.46e-12 67.0
MS.gene067705.t1 MTR_2g096190 23.611 288 185 7 48 312 105 380 1.82e-11 66.2
MS.gene067705.t1 MTR_4g062100 24.699 166 123 1 7 172 104 267 1.14e-11 67.0
MS.gene067705.t1 MTR_4g062100 22.917 288 187 4 48 312 109 384 2.01e-11 66.2
MS.gene067705.t1 MTR_5g085830 23.293 249 169 4 15 251 250 488 3.37e-11 65.5
MS.gene067705.t1 MTR_7g100390 25.490 357 206 15 10 312 12 362 6.47e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene067705.t1 AT4G10720 38.933 375 202 5 11 377 4 359 7.96e-81 255
MS.gene067705.t1 AT4G10720 38.046 389 219 5 11 377 4 392 4.43e-79 251
MS.gene067705.t1 AT1G14480 35.825 388 222 6 11 374 4 388 6.72e-75 240
MS.gene067705.t1 AT5G15500 37.592 407 218 8 12 397 6 397 7.54e-75 241
MS.gene067705.t1 AT1G14500 34.726 383 228 5 11 374 4 383 1.41e-70 229
MS.gene067705.t1 AT5G54620 40.468 299 172 2 18 310 11 309 1.95e-68 223
MS.gene067705.t1 AT5G54610 37.500 304 182 2 10 307 3 304 4.72e-68 222
MS.gene067705.t1 AT1G14480 32.979 376 206 8 11 374 4 345 1.62e-53 183
MS.gene067705.t1 AT5G15500 33.333 306 168 8 113 397 1 291 1.15e-35 134
MS.gene067705.t1 AT5G51160 28.000 325 204 6 65 369 34 348 1.81e-26 110
MS.gene067705.t1 AT4G03500 31.308 214 132 4 101 313 303 502 4.95e-20 92.8
MS.gene067705.t1 AT4G03500 31.308 214 132 4 101 313 280 479 5.35e-20 92.8
MS.gene067705.t1 AT4G03500 31.308 214 132 4 101 313 280 479 5.35e-20 92.8
MS.gene067705.t1 AT4G03460 31.481 216 124 5 103 313 290 486 5.18e-18 86.7
MS.gene067705.t1 AT4G03460 31.481 216 124 5 103 313 258 454 6.66e-18 86.3
MS.gene067705.t1 AT1G34050 26.437 261 169 6 68 316 174 423 4.89e-17 83.6
MS.gene067705.t1 AT4G03490 28.387 310 161 10 12 313 211 467 2.37e-16 81.6
MS.gene067705.t1 AT4G03490 28.387 310 161 10 12 313 211 467 3.57e-16 81.3
MS.gene067705.t1 AT4G03490 28.387 310 161 10 12 313 201 457 3.72e-16 80.9
MS.gene067705.t1 AT4G03480 24.036 337 183 13 12 334 357 634 2.48e-15 78.6
MS.gene067705.t1 AT1G10340 28.814 236 129 5 112 316 205 432 2.81e-15 78.2
MS.gene067705.t1 AT4G03480 24.036 337 183 13 12 334 360 637 2.95e-15 78.2
MS.gene067705.t1 AT1G10340 28.814 236 129 5 112 316 201 428 3.00e-15 78.2
MS.gene067705.t1 AT5G54700 28.475 295 141 11 27 320 20 245 4.16e-14 74.3
MS.gene067705.t1 AT5G54710 26.721 247 139 7 98 316 264 496 6.30e-14 74.3
MS.gene067705.t1 AT5G54710 26.721 247 139 7 98 316 219 451 6.59e-14 73.9
MS.gene067705.t1 AT1G07710 23.729 354 198 10 7 337 100 404 6.07e-13 70.9
MS.gene067705.t1 AT1G07710 23.729 354 198 10 7 337 100 404 6.07e-13 70.9
MS.gene067705.t1 AT2G01680 22.253 364 231 8 47 385 97 433 2.73e-12 68.9
MS.gene067705.t1 AT5G60070 25.301 166 121 2 2 166 102 265 1.58e-11 66.6
MS.gene067705.t1 AT1G03670 27.500 240 136 7 91 319 266 478 1.70e-11 66.6
MS.gene067705.t1 AT3G12360 23.641 368 215 9 11 313 77 443 2.19e-11 66.2
MS.gene067705.t1 AT3G12360 23.641 368 215 9 11 313 65 431 2.72e-11 65.9
MS.gene067705.t1 AT4G03450 22.866 328 199 8 12 335 227 504 8.15e-11 64.3

Find 78 sgRNAs with CRISPR-Local

Find 112 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACTGGTTAAAACAATTTA+TGG 0.118348 4.1:+68150664 None:intergenic
TTTACATATTGCTGCAACTA+TGG 0.135596 4.1:-68150537 MS.gene067705:CDS
TCAAGATGATCCATCCATTT+TGG 0.183666 4.1:-68150594 MS.gene067705:CDS
AGATTTGAAGTTAAAGAATT+TGG 0.216202 4.1:-68149780 MS.gene067705:CDS
CTTCACTTTGCAAGTCAAAT+TGG 0.229112 4.1:-68150332 MS.gene067705:CDS
AGCACAGGCACTTCTATTAT+TGG 0.259513 4.1:-68149819 MS.gene067705:intron
AAGATTATATTAATAAGTTT+TGG 0.269732 4.1:-68149716 MS.gene067705:CDS
TAGTGGCTTCTCCAATGTTT+TGG 0.292968 4.1:-68149286 MS.gene067705:CDS
TATATCTATATTCTCCAAAA+TGG 0.315194 4.1:+68150580 None:intergenic
ACTGATTTCCCAGCATTTCC+TGG 0.328259 4.1:+68149422 None:intergenic
AAAGACCTTGTTAGAGTTCA+AGG 0.330305 4.1:-68150374 MS.gene067705:CDS
AGAAACATCAATAAAGCTTA+TGG 0.354374 4.1:-68149073 MS.gene067705:CDS
TGGAGTAGAAATAGTAGAAT+TGG 0.356230 4.1:+68149442 None:intergenic
TCATAATCTCAGAGGCAAAC+TGG 0.386434 4.1:+68150507 None:intergenic
GTCTTGTCAGGATATGAATT+CGG 0.400138 4.1:-68149401 MS.gene067705:intron
TAGTGCTGAAATATGCAAAA+TGG 0.401032 4.1:+68150097 None:intergenic
GAGGCCCTTCAAGTACTTGT+TGG 0.407619 4.1:-68150200 MS.gene067705:CDS
AGGCCCTTCAAGTACTTGTT+GGG 0.409985 4.1:-68150199 MS.gene067705:CDS
GTCAAGTATTCAATGGATTC+CGG 0.412377 4.1:+68150272 None:intergenic
CTCAGGTCAAAGACAACAAT+TGG 0.414109 4.1:-68149644 MS.gene067705:CDS
ACAGAAAGGGGCTCAGGATC+TGG 0.416039 4.1:-68150159 MS.gene067705:CDS
CTTGAGCTGCAGCATTCAAC+TGG 0.428653 4.1:+68150648 None:intergenic
TCTTTAACTTCAAATCTAAA+CGG 0.434446 4.1:+68149786 None:intergenic
ATTTCTACTCCAGGAAATGC+TGG 0.442221 4.1:-68149431 MS.gene067705:CDS
GTTAGTGCTAGTGATGAAAA+TGG 0.446436 4.1:-68149482 MS.gene067705:CDS
ATTAATATAATCTTGATCTC+TGG 0.448509 4.1:+68149725 None:intergenic
TGTAAAGGAGTTTCAACAAA+TGG 0.448723 4.1:+68150554 None:intergenic
TCATGGAACTGACATGTGTA+GGG 0.460318 4.1:+68149225 None:intergenic
AGTAGAAATAGTAGAATTGG+AGG 0.467867 4.1:+68149445 None:intergenic
CTACATTTGCACATATTCTC+AGG 0.469728 4.1:-68149661 MS.gene067705:CDS
CTTCTATTATTGGTAAGTAC+TGG 0.476448 4.1:-68149809 MS.gene067705:CDS
GCTGGGAAATCAGTCTTGTC+AGG 0.477588 4.1:-68149413 MS.gene067705:CDS
TCAAAAGAAATGCACAGAAA+GGG 0.477992 4.1:-68150172 MS.gene067705:CDS
TGATCTCTGGAGAGCTTGCT+AGG 0.483666 4.1:+68149738 None:intergenic
TTTAGCTTCCACGCAAATGA+AGG 0.486065 4.1:+68150476 None:intergenic
CTTGTTAGAGTTCAAGGAAG+AGG 0.493464 4.1:-68150368 MS.gene067705:CDS
GCAACTGCGATCTATCAATC+AGG 0.505224 4.1:-68149533 MS.gene067705:CDS
CTCAAAAGAAATGCACAGAA+AGG 0.511597 4.1:-68150173 MS.gene067705:CDS
AAATGCACAGAAAGGGGCTC+AGG 0.513769 4.1:-68150165 MS.gene067705:CDS
TTACATATTGCTGCAACTAT+GGG 0.515504 4.1:-68150536 MS.gene067705:CDS
AATTCTACTATTTCTACTCC+AGG 0.522570 4.1:-68149440 MS.gene067705:CDS
TGTTAAGAATGGACAGTATG+AGG 0.523009 4.1:-68150219 MS.gene067705:CDS
AGCACTAAATAGTGACCTGC+AGG 0.525438 4.1:-68150081 MS.gene067705:intron
TCTCACAGTCAAGTATTCAA+TGG 0.527118 4.1:+68150265 None:intergenic
CCCTTCAAGTACTTGTTGGG+TGG 0.529806 4.1:-68150196 MS.gene067705:CDS
GCCACCCAACAAGTACTTGA+AGG 0.535655 4.1:+68150195 None:intergenic
TCTATATTCTCCAAAATGGA+TGG 0.540983 4.1:+68150584 None:intergenic
GCTTATGGTCATATAGATGA+TGG 0.541409 4.1:-68149058 MS.gene067705:CDS
TTGAATGCTGCAGCTCAAGC+AGG 0.545828 4.1:-68150644 MS.gene067705:CDS
ATTTGACTTGCAAAGTGAAG+AGG 0.547305 4.1:+68150335 None:intergenic
CTGCATGTTGCTGTTAAGAA+TGG 0.551550 4.1:-68150230 MS.gene067705:CDS
ATAGACGATGGACAAAATCA+TGG 0.552060 4.1:+68149208 None:intergenic
ATAGTTGCAGCAATATGTAA+AGG 0.555251 4.1:+68150539 None:intergenic
ATCATGGAACTGACATGTGT+AGG 0.555741 4.1:+68149224 None:intergenic
GATGCAACTCAAACAACTGC+TGG 0.556291 4.1:-68149025 MS.gene067705:CDS
CAGAAGTGCAACGTAACACT+TGG 0.562021 4.1:-68149577 MS.gene067705:CDS
GTTAGAGTTCAAGGAAGAGG+AGG 0.564832 4.1:-68150365 MS.gene067705:CDS
TTTCTACTCCAGGAAATGCT+GGG 0.579847 4.1:-68149430 MS.gene067705:CDS
GCATCAGTGACTTCTAAGCT+AGG 0.580948 4.1:+68149686 None:intergenic
TATTGAAACCTTCATTTGCG+TGG 0.582114 4.1:-68150484 MS.gene067705:CDS
CTAACCTGATATATTCCACC+AGG 0.583479 4.1:+68149500 None:intergenic
TGGTCATATAGATGATGGTA+CGG 0.588819 4.1:-68149053 MS.gene067705:CDS
CAAAAGAAATGCACAGAAAG+GGG 0.590009 4.1:-68150171 MS.gene067705:CDS
CATCTTGAATTACTGTGTAG+AGG 0.592515 4.1:+68150609 None:intergenic
CTCTTCCTTGAACTCTAACA+AGG 0.596189 4.1:+68150369 None:intergenic
CTAGCCACAAACCAAAACAT+TGG 0.596259 4.1:+68149275 None:intergenic
CCACCCAACAAGTACTTGAA+GGG 0.597674 4.1:+68150196 None:intergenic
CTGAGAATATGTGCAAATGT+AGG 0.615518 4.1:+68149662 None:intergenic
TGGAACAATAGGACTTCTAG+TGG 0.634853 4.1:-68149303 MS.gene067705:intron
CAGAACTGAACCATAGACGA+TGG 0.647697 4.1:+68149196 None:intergenic
AGAGCTTGCTAGGTCTAATG+CGG 0.657680 4.1:+68149748 None:intergenic
TTTCAATATCATAATCTCAG+AGG 0.659451 4.1:+68150499 None:intergenic
CATGGAACTGACATGTGTAG+GGG 0.679813 4.1:+68149226 None:intergenic
TAACCTGATATATTCCACCA+GGG 0.695982 4.1:+68149501 None:intergenic
GCGTGGAAGCTAAATCCACA+AGG 0.698133 4.1:-68150467 MS.gene067705:CDS
TGGATGGGACTGAATCCTTG+TGG 0.698413 4.1:+68150452 None:intergenic
AACCTGATATATTCCACCAG+GGG 0.698635 4.1:+68149502 None:intergenic
ACCTGATATATTCCACCAGG+GGG 0.735156 4.1:+68149503 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGATTATATTAATAAGTTT+TGG - chr4.1:68149967-68149986 MS.gene067705:intron 10.0%
!! AGACAACTTATAAATAAGAT+AGG - chr4.1:68149778-68149797 MS.gene067705:CDS 20.0%
!! ATTAATATAATCTTGATCTC+TGG + chr4.1:68149961-68149980 None:intergenic 20.0%
!! TATATCTATATTCTCCAAAA+TGG + chr4.1:68149106-68149125 None:intergenic 20.0%
!!! AATGTGTTCTTTTTTAGTAA+TGG + chr4.1:68149638-68149657 None:intergenic 20.0%
!!! AGATTTGAAGTTAAAGAATT+TGG - chr4.1:68149903-68149922 MS.gene067705:intron 20.0%
!!! CTAGTTATTTATTTTCTATG+TGG - chr4.1:68150427-68150446 MS.gene067705:CDS 20.0%
!!! TCATATTTCTTGTTACTTTT+TGG - chr4.1:68149714-68149733 MS.gene067705:CDS 20.0%
!!! TCTTTAACTTCAAATCTAAA+CGG + chr4.1:68149900-68149919 None:intergenic 20.0%
! AGAAACATCAATAAAGCTTA+TGG - chr4.1:68150610-68150629 MS.gene067705:CDS 25.0%
! CAACTGGTTAAAACAATTTA+TGG + chr4.1:68149022-68149041 None:intergenic 25.0%
! TGCTAAAATTGATACAGAAA+AGG + chr4.1:68150334-68150353 None:intergenic 25.0%
! TTTCAATATCATAATCTCAG+AGG + chr4.1:68149187-68149206 None:intergenic 25.0%
!! AGAATGAAATTTTAAACAGG+AGG - chr4.1:68149547-68149566 MS.gene067705:CDS 25.0%
!! GAATGAAATTTTAAACAGGA+GGG - chr4.1:68149548-68149567 MS.gene067705:CDS 25.0%
!! TGGAGAATGAAATTTTAAAC+AGG - chr4.1:68149544-68149563 MS.gene067705:CDS 25.0%
!! TGTATCGTTCTTTAATTTCT+TGG - chr4.1:68149840-68149859 MS.gene067705:intron 25.0%
!!! AGTTAAAGCATTGTTGAAAT+AGG - chr4.1:68150587-68150606 MS.gene067705:CDS 25.0%
!!! GTTATTTATTTTCTATGTGG+AGG - chr4.1:68150430-68150449 MS.gene067705:CDS 25.0%
AGTAGAAATAGTAGAATTGG+AGG + chr4.1:68150241-68150260 None:intergenic 30.0%
ATAGTTGCAGCAATATGTAA+AGG + chr4.1:68149147-68149166 None:intergenic 30.0%
CTTCTATTATTGGTAAGTAC+TGG - chr4.1:68149874-68149893 MS.gene067705:intron 30.0%
GCAAAACGACAAAAAACATA+TGG - chr4.1:68149263-68149282 MS.gene067705:CDS 30.0%
TCAAAAGAAATGCACAGAAA+GGG - chr4.1:68149511-68149530 MS.gene067705:CDS 30.0%
TCTATATTCTCCAAAATGGA+TGG + chr4.1:68149102-68149121 None:intergenic 30.0%
TGGAGTAGAAATAGTAGAAT+TGG + chr4.1:68150244-68150263 None:intergenic 30.0%
TGTAAAGGAGTTTCAACAAA+TGG + chr4.1:68149132-68149151 None:intergenic 30.0%
TTACATATTGCTGCAACTAT+GGG - chr4.1:68149147-68149166 MS.gene067705:intron 30.0%
TTTACATATTGCTGCAACTA+TGG - chr4.1:68149146-68149165 MS.gene067705:intron 30.0%
! GTTGTCTTTCATGTTTTTCA+AGG + chr4.1:68149765-68149784 None:intergenic 30.0%
! TAGTGCTGAAATATGCAAAA+TGG + chr4.1:68149589-68149608 None:intergenic 30.0%
!! AATTCTACTATTTCTACTCC+AGG - chr4.1:68150243-68150262 MS.gene067705:CDS 30.0%
!! CATAACAGTCATTTTCTTTC+AGG + chr4.1:68149666-68149685 None:intergenic 30.0%
AAAGACCTTGTTAGAGTTCA+AGG - chr4.1:68149309-68149328 MS.gene067705:intron 35.0%
ATAGACGATGGACAAAATCA+TGG + chr4.1:68150478-68150497 None:intergenic 35.0%
CAAAAGAAATGCACAGAAAG+GGG - chr4.1:68149512-68149531 MS.gene067705:CDS 35.0%
CAATAATGACATCAGTAACC+AGG + chr4.1:68150518-68150537 None:intergenic 35.0%
CATCTTGAATTACTGTGTAG+AGG + chr4.1:68149077-68149096 None:intergenic 35.0%
CTACATTTGCACATATTCTC+AGG - chr4.1:68150022-68150041 MS.gene067705:intron 35.0%
CTCAAAAGAAATGCACAGAA+AGG - chr4.1:68149510-68149529 MS.gene067705:CDS 35.0%
CTGAGAATATGTGCAAATGT+AGG + chr4.1:68150024-68150043 None:intergenic 35.0%
CTGGTTACTGATGTCATTAT+TGG - chr4.1:68150516-68150535 MS.gene067705:CDS 35.0%
CTTCACTTTGCAAGTCAAAT+TGG - chr4.1:68149351-68149370 MS.gene067705:intron 35.0%
GCTTATGGTCATATAGATGA+TGG - chr4.1:68150625-68150644 MS.gene067705:CDS 35.0%
GTCTTGTCAGGATATGAATT+CGG - chr4.1:68150282-68150301 MS.gene067705:CDS 35.0%
GTTAGTGCTAGTGATGAAAA+TGG - chr4.1:68150201-68150220 MS.gene067705:CDS 35.0%
TAACCTGATATATTCCACCA+GGG + chr4.1:68150185-68150204 None:intergenic 35.0%
TATTGAAACCTTCATTTGCG+TGG - chr4.1:68149199-68149218 MS.gene067705:CDS 35.0%
TCTCACAGTCAAGTATTCAA+TGG + chr4.1:68149421-68149440 None:intergenic 35.0%
TGTTAAGAATGGACAGTATG+AGG - chr4.1:68149464-68149483 MS.gene067705:CDS 35.0%
! AATTTTAAACAGGAGGGATG+AGG - chr4.1:68149554-68149573 MS.gene067705:CDS 35.0%
! ATGATTTTGTCCATCGTCTA+TGG - chr4.1:68150477-68150496 MS.gene067705:CDS 35.0%
! CTTTAATTTCTTGGTAGCAC+AGG - chr4.1:68149849-68149868 MS.gene067705:intron 35.0%
! GTCAAGTATTCAATGGATTC+CGG + chr4.1:68149414-68149433 None:intergenic 35.0%
! TCAAGATGATCCATCCATTT+TGG - chr4.1:68149089-68149108 MS.gene067705:intron 35.0%
! TGACTTTCTCTTTTTGTGTC+CGG - chr4.1:68149392-68149411 MS.gene067705:intron 35.0%
! TGGTCATATAGATGATGGTA+CGG - chr4.1:68150630-68150649 MS.gene067705:CDS 35.0%
!! ATTTGACTTGCAAAGTGAAG+AGG + chr4.1:68149351-68149370 None:intergenic 35.0%
!!! TTCTCCAATGTTTTGGTTTG+TGG - chr4.1:68150404-68150423 MS.gene067705:CDS 35.0%
AACCTGATATATTCCACCAG+GGG + chr4.1:68150184-68150203 None:intergenic 40.0%
AATGGCATAAGATTACCTGC+AGG + chr4.1:68149620-68149639 None:intergenic 40.0%
AGCACAGGCACTTCTATTAT+TGG - chr4.1:68149864-68149883 MS.gene067705:intron 40.0%
ATCATGGAACTGACATGTGT+AGG + chr4.1:68150462-68150481 None:intergenic 40.0%
ATTTCTACTCCAGGAAATGC+TGG - chr4.1:68150252-68150271 MS.gene067705:CDS 40.0%
CTAACCTGATATATTCCACC+AGG + chr4.1:68150186-68150205 None:intergenic 40.0%
CTAGCCACAAACCAAAACAT+TGG + chr4.1:68150411-68150430 None:intergenic 40.0%
CTCAGGTCAAAGACAACAAT+TGG - chr4.1:68150039-68150058 MS.gene067705:intron 40.0%
CTCTTCCTTGAACTCTAACA+AGG + chr4.1:68149317-68149336 None:intergenic 40.0%
CTTGTTAGAGTTCAAGGAAG+AGG - chr4.1:68149315-68149334 MS.gene067705:intron 40.0%
TCATAATCTCAGAGGCAAAC+TGG + chr4.1:68149179-68149198 None:intergenic 40.0%
TCATGGAACTGACATGTGTA+GGG + chr4.1:68150461-68150480 None:intergenic 40.0%
TCATTATTGGAGTGTGCAGA+AGG - chr4.1:68150529-68150548 MS.gene067705:CDS 40.0%
TGGAACAATAGGACTTCTAG+TGG - chr4.1:68150380-68150399 MS.gene067705:CDS 40.0%
TTTATGATACCACTACCACG+TGG - chr4.1:68150360-68150379 MS.gene067705:CDS 40.0%
TTTCTACTCCAGGAAATGCT+GGG - chr4.1:68150253-68150272 MS.gene067705:CDS 40.0%
! CTGCATGTTGCTGTTAAGAA+TGG - chr4.1:68149453-68149472 MS.gene067705:CDS 40.0%
! TAGTGGCTTCTCCAATGTTT+TGG - chr4.1:68150397-68150416 MS.gene067705:CDS 40.0%
! TTTAGCTTCCACGCAAATGA+AGG + chr4.1:68149210-68149229 None:intergenic 40.0%
! TTTTGCATAGCGAGATGGAT+GGG + chr4.1:68149249-68149268 None:intergenic 40.0%
ACCTGATATATTCCACCAGG+GGG + chr4.1:68150183-68150202 None:intergenic 45.0%
AGAAGTCCTATTGTTCCACG+TGG + chr4.1:68150378-68150397 None:intergenic 45.0%
AGCACTAAATAGTGACCTGC+AGG - chr4.1:68149602-68149621 MS.gene067705:CDS 45.0%
AGGCCCTTCAAGTACTTGTT+GGG - chr4.1:68149484-68149503 MS.gene067705:CDS 45.0%
CAGAACTGAACCATAGACGA+TGG + chr4.1:68150490-68150509 None:intergenic 45.0%
CATGGAACTGACATGTGTAG+GGG + chr4.1:68150460-68150479 None:intergenic 45.0%
CCACCCAACAAGTACTTGAA+GGG + chr4.1:68149490-68149509 None:intergenic 45.0%
GATGCAACTCAAACAACTGC+TGG - chr4.1:68150658-68150677 MS.gene067705:CDS 45.0%
GCAACTGCGATCTATCAATC+AGG - chr4.1:68150150-68150169 MS.gene067705:CDS 45.0%
GCATCAGTGACTTCTAAGCT+AGG + chr4.1:68150000-68150019 None:intergenic 45.0%
GTTAGAGTTCAAGGAAGAGG+AGG - chr4.1:68149318-68149337 MS.gene067705:intron 45.0%
TGGTTCAGTTCTGTTAGTCC+TGG - chr4.1:68150497-68150516 MS.gene067705:CDS 45.0%
! ACTGATTTCCCAGCATTTCC+TGG + chr4.1:68150264-68150283 None:intergenic 45.0%
! CAGAAGTGCAACGTAACACT+TGG - chr4.1:68150106-68150125 MS.gene067705:CDS 45.0%
! GTTTTGCATAGCGAGATGGA+TGG + chr4.1:68149250-68149269 None:intergenic 45.0%
!! AGAGCTTGCTAGGTCTAATG+CGG + chr4.1:68149938-68149957 None:intergenic 45.0%
!!! TGTCGTTTTGCATAGCGAGA+TGG + chr4.1:68149254-68149273 None:intergenic 45.0%
AAATGCACAGAAAGGGGCTC+AGG - chr4.1:68149518-68149537 MS.gene067705:CDS 50.0%
CCACTACCACGTGGAACAAT+AGG - chr4.1:68150369-68150388 MS.gene067705:CDS 50.0%
CCTATTGTTCCACGTGGTAG+TGG + chr4.1:68150372-68150391 None:intergenic 50.0%
CTTGAGCTGCAGCATTCAAC+TGG + chr4.1:68149038-68149057 None:intergenic 50.0%
GAGGCCCTTCAAGTACTTGT+TGG - chr4.1:68149483-68149502 MS.gene067705:CDS 50.0%
GCCACCCAACAAGTACTTGA+AGG + chr4.1:68149491-68149510 None:intergenic 50.0%
GCGTGGAAGCTAAATCCACA+AGG - chr4.1:68149216-68149235 MS.gene067705:CDS 50.0%
TCCCCCTGGTGGAATATATC+AGG - chr4.1:68150179-68150198 MS.gene067705:CDS 50.0%
TGGATGGGACTGAATCCTTG+TGG + chr4.1:68149234-68149253 None:intergenic 50.0%
TTGAATGCTGCAGCTCAAGC+AGG - chr4.1:68149039-68149058 MS.gene067705:CDS 50.0%
! CCCTTCAAGTACTTGTTGGG+TGG - chr4.1:68149487-68149506 MS.gene067705:CDS 50.0%
! GCTGGGAAATCAGTCTTGTC+AGG - chr4.1:68150270-68150289 MS.gene067705:CDS 50.0%
! TGATCTCTGGAGAGCTTGCT+AGG + chr4.1:68149948-68149967 None:intergenic 50.0%
ACAGAAAGGGGCTCAGGATC+TGG - chr4.1:68149524-68149543 MS.gene067705:CDS 55.0%
CAATCAGGATTGAGTCCCCC+TGG - chr4.1:68150165-68150184 MS.gene067705:CDS 55.0%
TCAGGATTGAGTCCCCCTGG+TGG - chr4.1:68150168-68150187 MS.gene067705:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 68149006 68150699 68149006 ID=MS.gene067705
chr4.1 mRNA 68149006 68150699 68149006 ID=MS.gene067705.t1;Parent=MS.gene067705
chr4.1 exon 68150082 68150699 68150082 ID=MS.gene067705.t1.exon1;Parent=MS.gene067705.t1
chr4.1 CDS 68150082 68150699 68150082 ID=cds.MS.gene067705.t1;Parent=MS.gene067705.t1
chr4.1 exon 68149402 68149834 68149402 ID=MS.gene067705.t1.exon2;Parent=MS.gene067705.t1
chr4.1 CDS 68149402 68149834 68149402 ID=cds.MS.gene067705.t1;Parent=MS.gene067705.t1
chr4.1 exon 68149197 68149314 68149197 ID=MS.gene067705.t1.exon3;Parent=MS.gene067705.t1
chr4.1 CDS 68149197 68149314 68149197 ID=cds.MS.gene067705.t1;Parent=MS.gene067705.t1
chr4.1 exon 68149006 68149096 68149006 ID=MS.gene067705.t1.exon4;Parent=MS.gene067705.t1
chr4.1 CDS 68149006 68149096 68149006 ID=cds.MS.gene067705.t1;Parent=MS.gene067705.t1
Gene Sequence

>MS.gene067705

ATGAACACAAATACCATAAATTGTTTTAACCAGTTGAATGCTGCAGCTCAAGCAGGTGATATAGATCTCCTCTACACAGTAATTCAAGATGATCCATCCATTTTGGAGAATATAGATATAATACCATTTGTTGAAACTCCTTTACATATTGCTGCAACTATGGGTCATCTCCAGTTTGCCTCTGAGATTATGATATTGAAACCTTCATTTGCGTGGAAGCTAAATCCACAAGGATTCAGTCCCATCCATCTCGCTATGCAAAACGACAAAAAACATATGGTGTATCGCTTTGTGTACATCAATAAAGACCTTGTTAGAGTTCAAGGAAGAGGAGGCTTAACTCCTCTTCACTTTGCAAGTCAAATTGGTGAGATTGAAATTTTAGCTGACTTTCTCTTTTTGTGTCCGGAATCCATTGAATACTTGACTGTGAGACGCGAGACTGCACTGCATGTTGCTGTTAAGAATGGACAGTATGAGGCCCTTCAAGTACTTGTTGGGTGGCTCAAAAGAAATGCACAGAAAGGGGCTCAGGATCTGGAGAATGAAATTTTAAACAGGAGGGATGAGGAACACAACACCATTTTGCATATTTCAGCACTAAATAGTGACCTGCAGGTAATCTTATGCCATTACTAAAAAAGAACACATTAAGAACCTGAAAGAAAATGACTGTTATGTTTCTTTTTATTTTGTTGTGAATGATGATCATATTTCTTGTTACTTTTTGGTCTTCTACTTCTTCGTCATCTTTTTCCTTGAAAAACATGAAAGACAACTTATAAATAAGATAGGTTGCTTAGTTGCTAGCTAACATGAATCATCTCTAACAATTGTATCGTTCTTTAATTTCTTGGTAGCACAGGCACTTCTATTATTGGTAAGTACTGGCCGTTTAGATTTGAAGTTAAAGAATTTGGAAAACAAAACCGCATTAGACCTAGCAAGCTCTCCAGAGATCAAGATTATATTAATAAGTTTTGGAGCAAAACCTAGCTTAGAAGTCACTGATGCTCCTACATTTGCACATATTCTCAGGTCAAAGACAACAATTGGATATAATATGAGAGTTTTTAAAACTCGCATTAATAGAAACATATCAGAAGTGCAACGTAACACTTGGTTGATAGTTGCGACTCTTGTTGCAACTGCGATCTATCAATCAGGATTGAGTCCCCCTGGTGGAATATATCAGGTTAGTGCTAGTGATGAAAATGGTGTCAACATCACTTCCTCCAATTCTACTATTTCTACTCCAGGAAATGCTGGGAAATCAGTCTTGTCAGGATATGAATTCGGTTTGTTTTTAATTGTAAATATGTATTCCTTTTCTGTATCAATTTTAGCAATATTTTTTATGATACCACTACCACGTGGAACAATAGGACTTCTAGTGGCTTCTCCAATGTTTTGGTTTGTGGCTAGTTATTTATTTTCTATGTGGAGGATATCCCCTACACATGTCAGTTCCATGATTTTGTCCATCGTCTATGGTTCAGTTCTGTTAGTCCTGGTTACTGATGTCATTATTGGAGTGTGCAGAAGGCTTATACACAGAATTGCTAAGATATTTAATATGAAAGTTAAAGCATTGTTGAAATAGGAGAAACATCAATAAAGCTTATGGTCATATAGATGATGGTACGGAGATCGATGCAACTCAAACAACTGCTGGTCGAATTCACAACCAATAA
Protein sequence

>MS.gene067705.t1

MNTNTINCFNQLNAAAQAGDIDLLYTVIQDDPSILENIDIIPFVETPLHIAATMGHLQFASEIMILKPSFAWKLNPQGFSPIHLAMQNDKKHMVYRFVYINKDLVRVQGRGGLTPLHFASQIGEIEILADFLFLCPESIEYLTVRRETALHVAVKNGQYEALQVLVGWLKRNAQKGAQDLENEILNRRDEEHNTILHISALNSDLQALLLLVSTGRLDLKLKNLENKTALDLASSPEIKIILISFGAKPSLEVTDAPTFAHILRSKTTIGYNMRVFKTRINRNISEVQRNTWLIVATLVATAIYQSGLSPPGGIYQVSASDENGVNITSSNSTISTPGNAGKSVLSGYEFGLLVASPMFWFVASYLFSMWRISPTHVSSMILSIVYGSVLRNINKAYGHIDDGTEIDATQTTAGRIHNQ