AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene23811

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene23811.t1 MTR_8g073260 85.185 432 33 1 1 401 1 432 0.0 747
MS.gene23811.t1 MTR_8g073275 61.298 447 64 7 53 400 1 437 2.58e-174 495
MS.gene23811.t1 MTR_3g016210 53.468 447 149 7 1 399 1 436 3.26e-156 449
MS.gene23811.t1 MTR_8g072510 56.235 409 146 4 1 377 1 408 8.97e-154 443
MS.gene23811.t1 MTR_8g039620 61.141 368 130 6 1 360 1 363 3.01e-150 434
MS.gene23811.t1 MTR_1g059930 63.501 337 120 1 10 343 11 347 1.50e-147 427
MS.gene23811.t1 MTR_7g451420 66.883 308 102 0 10 317 8 315 1.68e-144 414
MS.gene23811.t1 MTR_8g069880 52.103 428 166 7 4 396 1 424 5.54e-136 398
MS.gene23811.t1 MTR_8g467780 49.664 447 173 6 1 403 1 439 9.18e-134 392
MS.gene23811.t1 MTR_7g021090 54.891 368 152 5 1 365 1 357 1.19e-132 388
MS.gene23811.t1 MTR_8g068990 59.050 337 119 4 10 341 11 333 1.87e-130 384
MS.gene23811.t1 MTR_8g062410 52.998 417 150 7 1 381 1 407 1.68e-129 381
MS.gene23811.t1 MTR_8g066670 52.619 420 163 5 9 395 1073 1489 1.15e-128 405
MS.gene23811.t1 MTR_8g040930 54.131 351 126 4 53 368 1 351 3.84e-122 360
MS.gene23811.t1 MTR_8g041240 54.131 351 126 4 53 368 1 351 3.84e-122 360
MS.gene23811.t1 MTR_3g105650 45.946 407 197 5 4 394 1 400 1.20e-118 353
MS.gene23811.t1 MTR_1g069110 27.114 343 202 9 14 341 21 330 8.86e-28 115
MS.gene23811.t1 MTR_7g099580 25.806 310 179 8 14 318 15 278 5.28e-25 105
MS.gene23811.t1 MTR_7g099570 25.969 258 167 5 14 256 12 260 2.00e-19 91.3
MS.gene23811.t1 MTR_2g101320 28.372 215 136 4 42 248 137 341 1.20e-18 89.0
MS.gene23811.t1 MTR_7g117620 26.829 287 176 7 46 310 119 393 1.39e-18 88.2
MS.gene23811.t1 MTR_1g115575 26.370 292 174 6 46 311 169 445 7.14e-18 86.3
MS.gene23811.t1 MTR_1g032100 25.078 319 186 9 46 331 94 392 6.83e-17 83.2
MS.gene23811.t1 MTR_2g096190 25.347 288 179 7 47 310 105 380 7.68e-16 80.1
MS.gene23811.t1 MTR_7g100430 26.284 331 183 13 44 324 124 443 1.38e-15 79.3
MS.gene23811.t1 MTR_2g101310 27.189 217 138 4 42 249 137 342 1.46e-15 79.3
MS.gene23811.t1 MTR_2g101310 27.189 217 138 4 42 249 137 342 1.71e-15 78.6
MS.gene23811.t1 MTR_4g062100 24.306 288 182 6 47 310 109 384 1.07e-14 76.3
MS.gene23811.t1 MTR_4g062100 24.528 159 118 1 13 171 111 267 2.18e-11 66.2
MS.gene23811.t1 MTR_8g105260 24.138 290 183 5 46 310 101 378 9.24e-14 73.6
MS.gene23811.t1 MTR_7g100390 23.958 288 184 5 46 310 87 362 6.52e-13 70.9
MS.gene23811.t1 MTR_5g085500 27.879 165 107 4 73 233 1 157 4.11e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene23811.t1 AT4G10720 40.758 422 207 9 7 409 1 398 6.52e-99 301
MS.gene23811.t1 AT4G10720 39.756 410 215 6 7 384 1 410 1.88e-97 299
MS.gene23811.t1 AT1G14500 38.442 385 210 5 7 366 1 383 4.41e-83 262
MS.gene23811.t1 AT1G14480 37.046 413 221 8 7 384 1 409 1.26e-79 253
MS.gene23811.t1 AT5G15500 38.303 389 208 8 11 390 6 371 1.82e-79 253
MS.gene23811.t1 AT5G54620 41.667 312 169 4 7 308 1 309 1.55e-75 242
MS.gene23811.t1 AT5G54610 40.850 306 172 3 7 305 1 304 7.06e-74 238
MS.gene23811.t1 AT1G14480 36.296 405 192 12 7 384 1 366 1.80e-61 204
MS.gene23811.t1 AT5G15500 32.986 288 161 8 112 390 1 265 1.12e-38 143
MS.gene23811.t1 AT5G51160 31.317 281 161 6 59 317 31 301 8.63e-37 140
MS.gene23811.t1 AT1G10340 24.740 384 179 10 45 330 73 444 1.05e-17 85.9
MS.gene23811.t1 AT1G10340 29.297 256 133 7 111 330 205 448 2.76e-17 84.7
MS.gene23811.t1 AT3G09550 26.117 291 174 7 47 311 182 457 2.18e-16 81.6
MS.gene23811.t1 AT3G09550 26.117 291 174 7 47 311 182 457 2.18e-16 81.6
MS.gene23811.t1 AT3G09550 26.117 291 174 7 47 311 189 464 2.31e-16 81.6
MS.gene23811.t1 AT1G05640 25.436 287 180 6 46 310 191 465 7.58e-16 80.1
MS.gene23811.t1 AT2G01680 23.548 310 200 5 46 330 97 394 9.48e-16 79.7
MS.gene23811.t1 AT4G03490 25.672 409 217 17 45 395 180 559 1.08e-15 79.7
MS.gene23811.t1 AT4G03490 25.672 409 217 17 45 395 170 549 1.28e-15 79.3
MS.gene23811.t1 AT4G03490 25.672 409 217 17 45 395 180 559 1.44e-15 79.3
MS.gene23811.t1 AT1G34050 23.529 272 186 4 55 314 162 423 1.86e-15 79.0
MS.gene23811.t1 AT5G54700 26.299 308 150 9 26 328 20 255 2.78e-15 78.2
MS.gene23811.t1 AT2G31820 24.390 287 183 6 46 310 227 501 3.24e-15 78.2
MS.gene23811.t1 AT2G24600 25.731 342 207 11 26 330 117 448 3.73e-15 78.2
MS.gene23811.t1 AT2G24600 25.731 342 207 11 26 330 117 448 3.73e-15 78.2
MS.gene23811.t1 AT5G02620 25.086 291 179 6 47 310 95 373 3.74e-15 77.8
MS.gene23811.t1 AT5G02620 25.086 291 179 6 47 310 95 373 3.74e-15 77.8
MS.gene23811.t1 AT5G02620 25.086 291 179 6 47 310 95 373 3.74e-15 77.8
MS.gene23811.t1 AT4G03500 23.988 321 155 9 45 311 194 479 5.19e-15 77.8
MS.gene23811.t1 AT4G03500 23.988 321 155 9 45 311 194 479 5.19e-15 77.8
MS.gene23811.t1 AT4G03500 23.988 321 155 9 45 311 217 502 5.62e-15 77.4
MS.gene23811.t1 AT2G24600 27.338 278 161 9 70 314 163 432 1.19e-14 76.3
MS.gene23811.t1 AT2G24600 27.338 278 161 9 70 314 163 432 1.19e-14 76.3
MS.gene23811.t1 AT5G50140 27.317 205 123 5 42 230 26 220 1.83e-14 75.9
MS.gene23811.t1 AT5G54710 25.614 285 155 10 67 314 187 451 1.85e-14 75.9
MS.gene23811.t1 AT5G54710 25.614 285 155 10 67 314 232 496 2.21e-14 75.5
MS.gene23811.t1 AT4G03460 26.971 241 122 7 96 316 284 490 2.42e-14 75.5
MS.gene23811.t1 AT4G03460 26.971 241 122 7 96 316 252 458 2.59e-14 75.5
MS.gene23811.t1 AT3G12360 23.939 330 173 10 44 311 118 431 1.54e-13 72.8
MS.gene23811.t1 AT3G12360 23.939 330 173 10 44 311 130 443 1.74e-13 72.8
MS.gene23811.t1 AT5G02620 23.567 314 178 7 47 310 95 396 9.36e-12 67.4
MS.gene23811.t1 AT5G02620 23.567 314 178 7 47 310 95 396 9.36e-12 67.4
MS.gene23811.t1 AT4G03480 22.222 288 172 8 47 311 357 615 4.08e-11 65.5
MS.gene23811.t1 AT4G03480 22.222 288 172 8 47 311 360 618 4.13e-11 65.5
MS.gene23811.t1 AT1G07710 24.126 286 182 7 48 310 106 379 4.74e-11 65.1
MS.gene23811.t1 AT1G07710 24.126 286 182 7 48 310 106 379 4.74e-11 65.1

Find 76 sgRNAs with CRISPR-Local

Find 158 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTAACATCTTCGATCGAATT+TGG 0.197403 8.4:-30960393 None:intergenic
TCATAATTTCAGTGGCAAAT+TGG 0.236402 8.4:-30960158 None:intergenic
GAAATTAATACTTAATTATA+AGG 0.240344 8.4:+30960530 MS.gene23811:CDS
AGCACTAAACAATGACTTAA+AGG 0.246988 8.4:+30960584 MS.gene23811:CDS
CACCACTTCTTTGAATTCTA+AGG 0.264459 8.4:+30961406 MS.gene23811:CDS
TGAGGAGGATCCATATGTTT+TGG 0.280435 8.4:+30960071 MS.gene23811:CDS
TATTGTTCACACCAATCTTT+TGG 0.280558 8.4:+30961552 MS.gene23811:CDS
GTTTGTCGTGTAGATCATTA+AGG 0.287268 8.4:+30962136 MS.gene23811:intron
TCTTAATTTATCTTCAAGTT+TGG 0.313621 8.4:-30961192 None:intergenic
TAGTAATATACCCTTGATTT+CGG 0.324028 8.4:-30961129 None:intergenic
CTCTTCATGTCTTGGTTGGT+TGG 0.334379 8.4:+30960469 MS.gene23811:CDS
AACGAAGCACCATACTCTTT+TGG 0.338315 8.4:-30960254 None:intergenic
TCGTTTCGTAGACATGAATA+AGG 0.350127 8.4:+30960272 MS.gene23811:CDS
TTCAACTTTCAACTTAGTTA+TGG 0.392441 8.4:+30961628 MS.gene23811:CDS
TCTATATATTCCAAAACATA+TGG 0.396171 8.4:-30960081 None:intergenic
GCAAAACAACCAAAAGAGTA+TGG 0.398876 8.4:+30960245 MS.gene23811:CDS
GATTGAAACCTTCATTTGCT+TGG 0.413951 8.4:+30960181 MS.gene23811:CDS
GTTTGAAGCTCTTCATGTCT+TGG 0.417575 8.4:+30960461 MS.gene23811:CDS
AGCAGCAAGTGCCGAAATCA+AGG 0.421794 8.4:+30961118 MS.gene23811:CDS
TCAGCCGCTGGTTGAGTTCA+AGG 0.425805 8.4:+30962202 MS.gene23811:CDS
GAAGCTCTTCATGTCTTGGT+TGG 0.429930 8.4:+30960465 MS.gene23811:CDS
TATTGTGTACAAGAGACTTA+AGG 0.437095 8.4:+30961697 MS.gene23811:CDS
TCTTGCACTCTAGTGTGTAT+TGG 0.440713 8.4:+30961666 MS.gene23811:CDS
GTGAAGGTTGCAACTCTAGA+AGG 0.445366 8.4:+30960021 MS.gene23811:CDS
TCTACATATAGCTGCATCAA+TGG 0.456263 8.4:+30960128 MS.gene23811:CDS
ATTGTTATGTCTATAATATG+AGG 0.459017 8.4:+30961582 MS.gene23811:CDS
CCAACTCCCTAGCACCTCTC+TGG 0.465296 8.4:-30960506 None:intergenic
TTTGTCGTGTAGATCATTAA+GGG 0.467893 8.4:+30962137 MS.gene23811:intron
CTTAATTATAAGGATGAGAA+AGG 0.474353 8.4:+30960540 MS.gene23811:CDS
CTTTGCATTCAAATTTACCT+TGG 0.475404 8.4:-30961066 None:intergenic
AAGGAACTTGTTAGAATCAA+AGG 0.479455 8.4:+30960291 MS.gene23811:CDS
TGTAGAGGAGTTTCAACAAA+TGG 0.480280 8.4:-30960111 None:intergenic
CTTCATCTTGCTTGTCAAAG+TGG 0.481775 8.4:+30960333 MS.gene23811:CDS
TTCCTTAGAATTCAAAGAAG+TGG 0.484038 8.4:-30961408 None:intergenic
AAAGTTGAAGTGGCAGAGGT+AGG 0.486315 8.4:-30961614 None:intergenic
TTCACAGGCGCTTCGACTGT+TGG 0.486468 8.4:+30961037 MS.gene23811:intron
CCAGAGAGGTGCTAGGGAGT+TGG 0.487334 8.4:+30960506 MS.gene23811:CDS
CAATAAGCAAACCAAAAGAT+TGG 0.499253 8.4:-30961563 None:intergenic
TTTAGCTTCCAAGCAAATGA+AGG 0.500060 8.4:-30960189 None:intergenic
GTTGAAAGTTGAAGTGGCAG+AGG 0.510110 8.4:-30961618 None:intergenic
CTACATATAGCTGCATCAAT+GGG 0.510700 8.4:+30960129 MS.gene23811:CDS
AAGGGTATATTACTAAGTGC+TGG 0.522957 8.4:+30961137 MS.gene23811:CDS
AACTAAGTTGAAAGTTGAAG+TGG 0.534230 8.4:-30961624 None:intergenic
CAAATCGCCAGAGAGGTGCT+AGG 0.534314 8.4:+30960499 MS.gene23811:CDS
GTTAGAATCAAAGGAAGGGA+AGG 0.542029 8.4:+30960300 MS.gene23811:CDS
AAATCGCCAGAGAGGTGCTA+GGG 0.545317 8.4:+30960500 MS.gene23811:CDS
TAAGGGCATAAGCTGTCGAC+TGG 0.545452 8.4:+30962154 MS.gene23811:CDS
CCTTCTCTATACACTAATTG+AGG 0.550283 8.4:+30960053 MS.gene23811:CDS
TTTCAATCTCATAATTTCAG+TGG 0.552567 8.4:-30960166 None:intergenic
CCAAGCTCAAAAGTCAAAGA+TGG 0.559102 8.4:+30961167 MS.gene23811:CDS
CTTTGACAAGCAAGATGAAG+AGG 0.564320 8.4:-30960330 None:intergenic
GGTAAATCAGTTATTTCTGA+AGG 0.565613 8.4:+30961434 MS.gene23811:CDS
CGAGACTGCAACTCAGCCGC+TGG 0.566655 8.4:+30962190 MS.gene23811:CDS
TCTTTGAATTCTAAGGAAGT+CGG 0.568904 8.4:+30961413 MS.gene23811:CDS
CAGCCGCTGGTTGAGTTCAA+GGG 0.571676 8.4:+30962203 MS.gene23811:CDS
GATCGAAGATGTTACTCTGA+GGG 0.576263 8.4:+30960401 MS.gene23811:CDS
CCTCAATTAGTGTATAGAGA+AGG 0.577645 8.4:-30960053 None:intergenic
AACTTGTTAGAATCAAAGGA+AGG 0.583415 8.4:+30960295 MS.gene23811:CDS
TTACCTTGATAAACTCCACC+AGG 0.589003 8.4:-30961353 None:intergenic
GCTTGGAAGCTAAATCAACA+AGG 0.604414 8.4:+30960198 MS.gene23811:CDS
ACTTGTTAGAATCAAAGGAA+GGG 0.615429 8.4:+30960296 MS.gene23811:CDS
GCAGCAAGTGCCGAAATCAA+GGG 0.615626 8.4:+30961119 MS.gene23811:CDS
TGATATTGATTACAAAGTGA+AGG 0.624755 8.4:+30960005 MS.gene23811:CDS
CTTGGAAGCTAAATCAACAA+GGG 0.626538 8.4:+30960199 MS.gene23811:CDS
ATTGATGCAGCTATATGTAG+AGG 0.639713 8.4:-30960126 None:intergenic
CGATCGAAGATGTTACTCTG+AGG 0.644610 8.4:+30960400 MS.gene23811:CDS
ATCGAAGATGTTACTCTGAG+GGG 0.654189 8.4:+30960402 MS.gene23811:CDS
TCTCTATACACTAATTGAGG+AGG 0.662445 8.4:+30960056 MS.gene23811:CDS
TACCTTGATAAACTCCACCA+GGG 0.669953 8.4:-30961352 None:intergenic
TCGACTGTTGGTAAAGACCA+AGG 0.673714 8.4:+30961049 MS.gene23811:CDS
ACCTTGATAAACTCCACCAG+GGG 0.689637 8.4:-30961351 None:intergenic
TATTAGAAAAGATATATCAG+AGG 0.690330 8.4:+30961259 MS.gene23811:CDS
TTGAAGACAAATCGCCAGAG+AGG 0.694700 8.4:+30960492 MS.gene23811:CDS
AGTTTATCAAGGTAATGCTG+AGG 0.698798 8.4:+30961361 MS.gene23811:CDS
CTTCCCTTGAACTCAACCAG+CGG 0.704211 8.4:-30962206 None:intergenic
CCTTGATAAACTCCACCAGG+GGG 0.739127 8.4:-30961350 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAATATACTAAATAGA+AGG - chr8.4:30960613-30960632 None:intergenic 10.0%
!! GAAATTAATACTTAATTATA+AGG + chr8.4:30960530-30960549 MS.gene23811:CDS 10.0%
!! AAGTGTATTCAAAATTGATA+AGG - chr8.4:30961471-30961490 None:intergenic 20.0%
!! ACAAATAAAGAGATATAAAG+AGG - chr8.4:30960865-30960884 None:intergenic 20.0%
!! ATCAAAATTAGGAATAGATT+AGG + chr8.4:30961740-30961759 MS.gene23811:intron 20.0%
!! ATTGTTATGTCTATAATATG+AGG + chr8.4:30961582-30961601 MS.gene23811:CDS 20.0%
!! CAAATAAAAAGTTAGCTAAA+AGG - chr8.4:30960368-30960387 None:intergenic 20.0%
!! TATTAGAAAAGATATATCAG+AGG + chr8.4:30961259-30961278 MS.gene23811:CDS 20.0%
!! TCTATATATTCCAAAACATA+TGG - chr8.4:30960084-30960103 None:intergenic 20.0%
!!! ATAACAACTATTTTATTCAC+AGG + chr8.4:30961022-30961041 MS.gene23811:intron 20.0%
!!! TCTTAATTTATCTTCAAGTT+TGG - chr8.4:30961195-30961214 None:intergenic 20.0%
!!! TTATTTTTGATGAATGACTA+TGG + chr8.4:30960628-30960647 MS.gene23811:intron 20.0%
!!! TTTAAGCAATTTTCAAACAT+CGG + chr8.4:30962050-30962069 MS.gene23811:intron 20.0%
! AAAATTAGGAATAGATTAGG+AGG + chr8.4:30961743-30961762 MS.gene23811:intron 25.0%
! ATAGATTAGGAGGAAATAAA+TGG + chr8.4:30961753-30961772 MS.gene23811:intron 25.0%
! CTTAATTATAAGGATGAGAA+AGG + chr8.4:30960540-30960559 MS.gene23811:CDS 25.0%
! TACTAGCATTATTACTTACA+AGG - chr8.4:30961894-30961913 None:intergenic 25.0%
! TAGTAATATACCCTTGATTT+CGG - chr8.4:30961132-30961151 None:intergenic 25.0%
! TGTCAAAAGATATATTGTCA+AGG - chr8.4:30961510-30961529 None:intergenic 25.0%
! TTCAACTTTCAACTTAGTTA+TGG + chr8.4:30961628-30961647 MS.gene23811:CDS 25.0%
! TTTCAATCTCATAATTTCAG+TGG - chr8.4:30960169-30960188 None:intergenic 25.0%
!! AAGATCAAATGTCACATTTT+TGG + chr8.4:30961211-30961230 MS.gene23811:CDS 25.0%
!! TGATATTGATTACAAAGTGA+AGG + chr8.4:30960005-30960024 MS.gene23811:CDS 25.0%
!!! ATATAGTCCTAGTTTTTTGA+CGG - chr8.4:30960784-30960803 None:intergenic 25.0%
!!! GATATGTTAGTTCTAAATTG+AGG + chr8.4:30960925-30960944 MS.gene23811:intron 25.0%
AAGAGGACATGTTAGAAAAA+TGG - chr8.4:30960848-30960867 None:intergenic 30.0%
AGCACTAAACAATGACTTAA+AGG + chr8.4:30960584-30960603 MS.gene23811:CDS 30.0%
AGGATAGGGAAATCAAAATT+AGG + chr8.4:30961729-30961748 MS.gene23811:intron 30.0%
CAATAAGCAAACCAAAAGAT+TGG - chr8.4:30961566-30961585 None:intergenic 30.0%
CATGTGACATCACAAATTAT+AGG - chr8.4:30960888-30960907 None:intergenic 30.0%
CATTATTACTTACAAGGAAC+GGG - chr8.4:30961888-30961907 None:intergenic 30.0%
CTTTGCATTCAAATTTACCT+TGG - chr8.4:30961069-30961088 None:intergenic 30.0%
GCATTATTACTTACAAGGAA+CGG - chr8.4:30961889-30961908 None:intergenic 30.0%
GCTTGCAAAGATTTAGTTAT+CGG + chr8.4:30960984-30961003 MS.gene23811:intron 30.0%
TAAGGTTAATGGAAAGGATA+GGG + chr8.4:30961715-30961734 MS.gene23811:intron 30.0%
TACAAGAGACTTAAGGTTAA+TGG + chr8.4:30961704-30961723 MS.gene23811:intron 30.0%
TATTGTGTACAAGAGACTTA+AGG + chr8.4:30961697-30961716 MS.gene23811:CDS 30.0%
TCATAATTTCAGTGGCAAAT+TGG - chr8.4:30960161-30960180 None:intergenic 30.0%
TTAAAAGTGACGATGATCAT+TGG + chr8.4:30962017-30962036 MS.gene23811:intron 30.0%
TTAAGGTTAATGGAAAGGAT+AGG + chr8.4:30961714-30961733 MS.gene23811:intron 30.0%
TTAGAAAAATGGACCTTCTT+AGG - chr8.4:30960837-30960856 None:intergenic 30.0%
TTCCTTAGAATTCAAAGAAG+TGG - chr8.4:30961411-30961430 None:intergenic 30.0%
TTTGTCGTGTAGATCATTAA+GGG + chr8.4:30962137-30962156 MS.gene23811:intron 30.0%
! AACTAAGTTGAAAGTTGAAG+TGG - chr8.4:30961627-30961646 None:intergenic 30.0%
! ACTTGTTAGAATCAAAGGAA+GGG + chr8.4:30960296-30960315 MS.gene23811:CDS 30.0%
! ATATATCTTTTGACACCAAG+TGG + chr8.4:30961515-30961534 MS.gene23811:intron 30.0%
! TATTGTTCACACCAATCTTT+TGG + chr8.4:30961552-30961571 MS.gene23811:CDS 30.0%
! TCTTTGAATTCTAAGGAAGT+CGG + chr8.4:30961413-30961432 MS.gene23811:CDS 30.0%
!! AACTTGTTAGAATCAAAGGA+AGG + chr8.4:30960295-30960314 MS.gene23811:CDS 30.0%
!! AAGGAACTTGTTAGAATCAA+AGG + chr8.4:30960291-30960310 MS.gene23811:CDS 30.0%
!! GGTAAATCAGTTATTTCTGA+AGG + chr8.4:30961434-30961453 MS.gene23811:CDS 30.0%
!!! TATAGTCCTAGTTTTTTGAC+GGG - chr8.4:30960783-30960802 None:intergenic 30.0%
!!! TCTTGTTTTTGTCGAATCAA+TGG + chr8.4:30961956-30961975 MS.gene23811:intron 30.0%
AAAACTAGGACTATATGCGT+TGG + chr8.4:30960788-30960807 MS.gene23811:intron 35.0%
AAACCACCTATTAATCCACT+TGG - chr8.4:30961533-30961552 None:intergenic 35.0%
AAACTGGTAAATTTGCAGCA+AGG - chr8.4:30962120-30962139 None:intergenic 35.0%
AGTTTATCAAGGTAATGCTG+AGG + chr8.4:30961361-30961380 MS.gene23811:CDS 35.0%
ATGGTATGATTGAACCAAGA+TGG + chr8.4:30960647-30960666 MS.gene23811:intron 35.0%
ATTGAACCAAGATGGTGAAA+CGG + chr8.4:30960655-30960674 MS.gene23811:intron 35.0%
ATTGATGCAGCTATATGTAG+AGG - chr8.4:30960129-30960148 None:intergenic 35.0%
CACCACTTCTTTGAATTCTA+AGG + chr8.4:30961406-30961425 MS.gene23811:CDS 35.0%
CCTCAATTAGTGTATAGAGA+AGG - chr8.4:30960056-30960075 None:intergenic 35.0%
CCTTCTCTATACACTAATTG+AGG + chr8.4:30960053-30960072 MS.gene23811:CDS 35.0%
CTACATATAGCTGCATCAAT+GGG + chr8.4:30960129-30960148 MS.gene23811:CDS 35.0%
CTTGGAAGCTAAATCAACAA+GGG + chr8.4:30960199-30960218 MS.gene23811:CDS 35.0%
GAAATGTCTCAAATGGTCTA+TGG + chr8.4:30961816-30961835 MS.gene23811:intron 35.0%
GAGAATTTGAACTGACAGAT+AGG - chr8.4:30960753-30960772 None:intergenic 35.0%
GAGACTTAAGGTTAATGGAA+AGG + chr8.4:30961709-30961728 MS.gene23811:intron 35.0%
GATTGAAACCTTCATTTGCT+TGG + chr8.4:30960181-30960200 MS.gene23811:CDS 35.0%
GCAAAACAACCAAAAGAGTA+TGG + chr8.4:30960245-30960264 MS.gene23811:CDS 35.0%
GTAACATCTTCGATCGAATT+TGG - chr8.4:30960396-30960415 None:intergenic 35.0%
GTAACTCAATCACAACTCAT+TGG + chr8.4:30961928-30961947 MS.gene23811:intron 35.0%
GTAGTTATAGCAATGTTGCA+AGG + chr8.4:30961775-30961794 MS.gene23811:intron 35.0%
GTTGCAAGGTGACTTATATA+GGG + chr8.4:30961789-30961808 MS.gene23811:intron 35.0%
GTTTGTCGTGTAGATCATTA+AGG + chr8.4:30962136-30962155 MS.gene23811:intron 35.0%
TAATGATCTACACGACAAAC+TGG - chr8.4:30962136-30962155 None:intergenic 35.0%
TCAAAAAAAACGAGACATGG+CGG - chr8.4:30960687-30960706 None:intergenic 35.0%
TCGTTTCGTAGACATGAATA+AGG + chr8.4:30960272-30960291 MS.gene23811:CDS 35.0%
TCTACATATAGCTGCATCAA+TGG + chr8.4:30960128-30960147 MS.gene23811:CDS 35.0%
TCTCTATACACTAATTGAGG+AGG + chr8.4:30960056-30960075 MS.gene23811:CDS 35.0%
TGTAGAGGAGTTTCAACAAA+TGG - chr8.4:30960114-30960133 None:intergenic 35.0%
TGTTGCAAGGTGACTTATAT+AGG + chr8.4:30961788-30961807 MS.gene23811:intron 35.0%
TTGTCGAATCAATGGTGATA+AGG + chr8.4:30961964-30961983 MS.gene23811:intron 35.0%
! AAAGTGTTCCACAAAGTCTA+AGG - chr8.4:30962080-30962099 None:intergenic 35.0%
! AAGGGTATATTACTAAGTGC+TGG + chr8.4:30961137-30961156 MS.gene23811:CDS 35.0%
! TCAATCCATTTTGCCTAAGA+AGG + chr8.4:30960821-30960840 MS.gene23811:intron 35.0%
! TTTAGCTTCCAAGCAAATGA+AGG - chr8.4:30960192-30960211 None:intergenic 35.0%
!! ACTCTTTTGGTTGTTTTGCA+TGG - chr8.4:30960244-30960263 None:intergenic 35.0%
!! TTGACACCAAGTGGATTAAT+AGG + chr8.4:30961524-30961543 MS.gene23811:intron 35.0%
!!! ATAGTCCTAGTTTTTTGACG+GGG - chr8.4:30960782-30960801 None:intergenic 35.0%
!!! CCATGTCTCGTTTTTTTTGA+GGG + chr8.4:30960687-30960706 MS.gene23811:intron 35.0%
ACCATGTCTCGTTTGTTTAG+CGG + chr8.4:30960712-30960731 MS.gene23811:intron 40.0%
ATCGAAGATGTTACTCTGAG+GGG + chr8.4:30960402-30960421 MS.gene23811:CDS 40.0%
ATGGACCTTCTTAGGCAAAA+TGG - chr8.4:30960829-30960848 None:intergenic 40.0%
ATGTCTCAAATGGTCTATGG+TGG + chr8.4:30961819-30961838 MS.gene23811:intron 40.0%
CCAAGCTCAAAAGTCAAAGA+TGG + chr8.4:30961167-30961186 MS.gene23811:CDS 40.0%
CCCTCAAAAAAAACGAGACA+TGG - chr8.4:30960690-30960709 None:intergenic 40.0%
CTTCATCTTGCTTGTCAAAG+TGG + chr8.4:30960333-30960352 MS.gene23811:CDS 40.0%
CTTTGACAAGCAAGATGAAG+AGG - chr8.4:30960333-30960352 None:intergenic 40.0%
GATCGAAGATGTTACTCTGA+GGG + chr8.4:30960401-30960420 MS.gene23811:CDS 40.0%
GCTTGGAAGCTAAATCAACA+AGG + chr8.4:30960198-30960217 MS.gene23811:CDS 40.0%
GTTAGAATCAAAGGAAGGGA+AGG + chr8.4:30960300-30960319 MS.gene23811:CDS 40.0%
GTTTGTTTAGCGGTTTAGGT+GGG + chr8.4:30960722-30960741 MS.gene23811:intron 40.0%
TACCTTGATAAACTCCACCA+GGG - chr8.4:30961355-30961374 None:intergenic 40.0%
TCGGTTAACCTTAGACTTTG+TGG + chr8.4:30962069-30962088 MS.gene23811:intron 40.0%
TCTCGTTTGTTTAGCGGTTT+AGG + chr8.4:30960718-30960737 MS.gene23811:intron 40.0%
TCTTGCACTCTAGTGTGTAT+TGG + chr8.4:30961666-30961685 MS.gene23811:CDS 40.0%
TTACCTTGATAAACTCCACC+AGG - chr8.4:30961356-30961375 None:intergenic 40.0%
TTGAACCAAGATGGTGAAAC+GGG + chr8.4:30960656-30960675 MS.gene23811:intron 40.0%
TTTGTTTAGCGGTTTAGGTG+GGG + chr8.4:30960723-30960742 MS.gene23811:intron 40.0%
! GTTTGAAGCTCTTCATGTCT+TGG + chr8.4:30960461-30960480 MS.gene23811:CDS 40.0%
! TGAGGAGGATCCATATGTTT+TGG + chr8.4:30960071-30960090 MS.gene23811:CDS 40.0%
!! AACGAAGCACCATACTCTTT+TGG - chr8.4:30960257-30960276 None:intergenic 40.0%
!! ACACCAAGTGGATTAATAGG+TGG + chr8.4:30961527-30961546 MS.gene23811:intron 40.0%
!! CAAAATGGATTGAGCCTGAT+GGG - chr8.4:30960814-30960833 None:intergenic 40.0%
!! CCATCTTTGACTTTTGAGCT+TGG - chr8.4:30961170-30961189 None:intergenic 40.0%
!! TTTGGTTGTTTTGCATGGCA+AGG - chr8.4:30960239-30960258 None:intergenic 40.0%
!!! GCCATGTCTCGTTTTTTTTG+AGG + chr8.4:30960686-30960705 MS.gene23811:intron 40.0%
AAAGTTGAAGTGGCAGAGGT+AGG - chr8.4:30961617-30961636 None:intergenic 45.0%
ACCGCTAAACAAACGAGACA+TGG - chr8.4:30960716-30960735 None:intergenic 45.0%
ACCTTGATAAACTCCACCAG+GGG - chr8.4:30961354-30961373 None:intergenic 45.0%
CGATCGAAGATGTTACTCTG+AGG + chr8.4:30960400-30960419 MS.gene23811:CDS 45.0%
CGTTTGTTTAGCGGTTTAGG+TGG + chr8.4:30960721-30960740 MS.gene23811:intron 45.0%
CTCTTCATGTCTTGGTTGGT+TGG + chr8.4:30960469-30960488 MS.gene23811:CDS 45.0%
GAAGCTCTTCATGTCTTGGT+TGG + chr8.4:30960465-30960484 MS.gene23811:CDS 45.0%
GCAAGGTGACTTATATAGGG+TGG + chr8.4:30961792-30961811 MS.gene23811:intron 45.0%
GGGTGGAGAAATGTCTCAAA+TGG + chr8.4:30961809-30961828 MS.gene23811:intron 45.0%
GTGAAGGTTGCAACTCTAGA+AGG + chr8.4:30960021-30960040 MS.gene23811:CDS 45.0%
GTTGAAAGTTGAAGTGGCAG+AGG - chr8.4:30961621-30961640 None:intergenic 45.0%
TGATAAGGTCACTATCGTGG+AGG + chr8.4:30961979-30961998 MS.gene23811:intron 45.0%
TGGTGATAAGGTCACTATCG+TGG + chr8.4:30961976-30961995 MS.gene23811:intron 45.0%
TTGAAGACAAATCGCCAGAG+AGG + chr8.4:30960492-30960511 MS.gene23811:CDS 45.0%
! TCGACTGTTGGTAAAGACCA+AGG + chr8.4:30961049-30961068 MS.gene23811:CDS 45.0%
! TTTTGCATGGCAAGGTGGAT+TGG - chr8.4:30960231-30960250 None:intergenic 45.0%
!! AAAAACGAGACATGGCGGAT+AGG - chr8.4:30960682-30960701 None:intergenic 45.0%
!! GCAAAATGGATTGAGCCTGA+TGG - chr8.4:30960815-30960834 None:intergenic 45.0%
ACTCGCCCCGTCAAAAAACT+AGG + chr8.4:30960774-30960793 MS.gene23811:intron 50.0%
ATAGGCCCGTTTCACCATCT+TGG - chr8.4:30960664-30960683 None:intergenic 50.0%
CCTTGATAAACTCCACCAGG+GGG - chr8.4:30961353-30961372 None:intergenic 50.0%
CTTCCCTTGAACTCAACCAG+CGG - chr8.4:30962209-30962228 None:intergenic 50.0%
TTTAGCGGTTTAGGTGGGGA+GGG + chr8.4:30960727-30960746 MS.gene23811:intron 50.0%
! ACTATATGCGTTGGCCCATC+AGG + chr8.4:30960797-30960816 MS.gene23811:intron 50.0%
! TAAGGGCATAAGCTGTCGAC+TGG + chr8.4:30962154-30962173 MS.gene23811:CDS 50.0%
!! AAATCGCCAGAGAGGTGCTA+GGG + chr8.4:30960500-30960519 MS.gene23811:CDS 50.0%
!! AGCAGCAAGTGCCGAAATCA+AGG + chr8.4:30961118-30961137 MS.gene23811:CDS 50.0%
!! GCAGCAAGTGCCGAAATCAA+GGG + chr8.4:30961119-30961138 MS.gene23811:CDS 50.0%
!! GGTTGTTTTGCATGGCAAGG+TGG - chr8.4:30960236-30960255 None:intergenic 50.0%
CCCCCTGGTGGAGTTTATCA+AGG + chr8.4:30961350-30961369 MS.gene23811:CDS 55.0%
GTTTAGCGGTTTAGGTGGGG+AGG + chr8.4:30960726-30960745 MS.gene23811:intron 55.0%
TTCACAGGCGCTTCGACTGT+TGG + chr8.4:30961037-30961056 MS.gene23811:intron 55.0%
! CAGCCGCTGGTTGAGTTCAA+GGG + chr8.4:30962203-30962222 MS.gene23811:CDS 55.0%
! TCAGCCGCTGGTTGAGTTCA+AGG + chr8.4:30962202-30962221 MS.gene23811:CDS 55.0%
!! CAAATCGCCAGAGAGGTGCT+AGG + chr8.4:30960499-30960518 MS.gene23811:CDS 55.0%
CCAACTCCCTAGCACCTCTC+TGG - chr8.4:30960509-30960528 None:intergenic 60.0%
! CAATCAGCACTGAGTCCCCC+TGG + chr8.4:30961335-30961354 MS.gene23811:CDS 60.0%
!! CCAGAGAGGTGCTAGGGAGT+TGG + chr8.4:30960506-30960525 MS.gene23811:CDS 60.0%
CGAGACTGCAACTCAGCCGC+TGG + chr8.4:30962190-30962209 MS.gene23811:CDS 65.0%
! TCAGCACTGAGTCCCCCTGG+TGG + chr8.4:30961338-30961357 MS.gene23811:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 30959991 30962235 30959991 ID=MS.gene23811
chr8.4 mRNA 30959991 30962235 30959991 ID=MS.gene23811.t1;Parent=MS.gene23811
chr8.4 exon 30959991 30960605 30959991 ID=MS.gene23811.t1.exon1;Parent=MS.gene23811.t1
chr8.4 CDS 30959991 30960605 30959991 ID=cds.MS.gene23811.t1;Parent=MS.gene23811.t1
chr8.4 exon 30961044 30961455 30961044 ID=MS.gene23811.t1.exon2;Parent=MS.gene23811.t1
chr8.4 CDS 30961044 30961455 30961044 ID=cds.MS.gene23811.t1;Parent=MS.gene23811.t1
chr8.4 exon 30961546 30961718 30961546 ID=MS.gene23811.t1.exon3;Parent=MS.gene23811.t1
chr8.4 CDS 30961546 30961718 30961546 ID=cds.MS.gene23811.t1;Parent=MS.gene23811.t1
chr8.4 exon 30962149 30962235 30962149 ID=MS.gene23811.t1.exon4;Parent=MS.gene23811.t1
chr8.4 CDS 30962149 30962235 30962149 ID=cds.MS.gene23811.t1;Parent=MS.gene23811.t1
Gene Sequence

>MS.gene23811

ATGAACACAATGAATGATATTGATTACAAAGTGAAGGTTGCAACTCTAGAAGGTGATATAAACCTTCTCTATACACTAATTGAGGAGGATCCATATGTTTTGGAATATATAGATTTCATACCATTTGTTGAAACTCCTCTACATATAGCTGCATCAATGGGACATGTCCAATTTGCCACTGAAATTATGAGATTGAAACCTTCATTTGCTTGGAAGCTAAATCAACAAGGGTTCAGTCCAATCCACCTTGCCATGCAAAACAACCAAAAGAGTATGGTGCTTCGTTTCGTAGACATGAATAAGGAACTTGTTAGAATCAAAGGAAGGGAAGGTTTAACTCCTCTTCATCTTGCTTGTCAAAGTGGAGAAATTGACCTTTTAGCTAACTTTTTATTTGTTTGTCCAAATTCGATCGAAGATGTTACTCTGAGGGGTGAGACTGCACTTCATATTTCTGTCAAAAATAAACAGTTTGAAGCTCTTCATGTCTTGGTTGGTTGGTTGAAGACAAATCGCCAGAGAGGTGCTAGGGAGTTGGAGAAATTAATACTTAATTATAAGGATGAGAAAGGAAACACTATTTTGCATATTTCAGCACTAAACAATGACTTAAAGGTAACCTTCTATTTAGTATATTTTATTTTTGATGAATGACTATGGTATGATTGAACCAAGATGGTGAAACGGGCCTATCCGCCATGTCTCGTTTTTTTTGAGGGAAACCATGTCTCGTTTGTTTAGCGGTTTAGGTGGGGAGGGCCTATCTGTCAGTTCAAATTCTCAACTCGCCCCGTCAAAAAACTAGGACTATATGCGTTGGCCCATCAGGCTCAATCCATTTTGCCTAAGAAGGTCCATTTTTCTAACATGTCCTCTTTATATCTCTTTATTTGTCCTATAATTTGTGATGTCACATGAAAGAAAAGACATAACAGATATGTTAGTTCTAAATTGAGGCTGAGATGCTGTTAGAGTCTTTTAAAACTATTACTTGCTTGCAAAGATTTAGTTATCGGTTAAAGAAATGATTGATAACAACTATTTTATTCACAGGCGCTTCGACTGTTGGTAAAGACCAAGGTAAATTTGAATGCAAAGAATTCAGAGAACTCAACAGCATTAGACTTAGCAGCAAGTGCCGAAATCAAGGGTATATTACTAAGTGCTGGAGCAAAACCAAGCTCAAAAGTCAAAGATGGCTCCAAACTTGAAGATAAATTAAGATCAAATGTCACATTTTTGGATAAAATGTTAATTTATATACTGCGTATTAGAAAAGATATATCAGAGGAACAACGCAACGCTTTTCTAATAGTTGCTACTCTTATTGCAACTGCTACATATCAATCAGCACTGAGTCCCCCTGGTGGAGTTTATCAAGGTAATGCTGAGGATCACAATAATAATGTGAAAAACACCACTTCTTTGAATTCTAAGGAAGTCGGTAAATCAGTTATTTCTGAAGGTGATTTTTTTACCTTATCAATTTTGAATACACTTTCACTTCTGTTATCAACCTTGACAATATATCTTTTGACACCAAGTGGATTAATAGGTGGTTTATTGTTCACACCAATCTTTTGGTTTGCTTATTGTTATGTCTATAATATGAGGTTGATATCTCCTACCTCTGCCACTTCAACTTTCAACTTAGTTATGGTGCATGTATTCAACTTCTTGCACTCTAGTGTGTATTGGTCAATTTTTATTGTGTACAAGAGACTTAAGGTTAATGGAAAGGATAGGGAAATCAAAATTAGGAATAGATTAGGAGGAAATAAATGGTAGTTATAGCAATGTTGCAAGGTGACTTATATAGGGTGGAGAAATGTCTCAAATGGTCTATGGTGGATCGATGCTTTAAAATAGTTTGAAACTTTAAGAACAACTGATGCCCGTTCCTTGTAAGTAATAATGCTAGTATTACTTGTTCTCGAGTAACTCAATCACAACTCATTGGTTTTATCTTGTTTTTGTCGAATCAATGGTGATAAGGTCACTATCGTGGAGGATCTTAAACTTCTACTTAAAAGTGACGATGATCATTGGTTGTCAAAATTTTAAGCAATTTTCAAACATCGGTTAACCTTAGACTTTGTGGAACACTTTTCACACTCAAGCTGTTGCCTTGCTGCAAATTTACCAGTTTGTCGTGTAGATCATTAAGGGCATAAGCTGTCGACTGGATCATTGCTGCTACGAGACTGCAACTCAGCCGCTGGTTGAGTTCAAGGGAAGCAATTGA
Protein sequence

>MS.gene23811.t1

MNTMNDIDYKVKVATLEGDINLLYTLIEEDPYVLEYIDFIPFVETPLHIAASMGHVQFATEIMRLKPSFAWKLNQQGFSPIHLAMQNNQKSMVLRFVDMNKELVRIKGREGLTPLHLACQSGEIDLLANFLFVCPNSIEDVTLRGETALHISVKNKQFEALHVLVGWLKTNRQRGARELEKLILNYKDEKGNTILHISALNNDLKALRLLVKTKVNLNAKNSENSTALDLAASAEIKGILLSAGAKPSSKVKDGSKLEDKLRSNVTFLDKMLIYILRIRKDISEEQRNAFLIVATLIATATYQSALSPPGGVYQGNAEDHNNNVKNTTSLNSKEVGKSVISEGGLLFTPIFWFAYCYVYNMRLISPTSATSTFNLVMVHVFNFLHSSVYWSIFIVYKRLKIIKGISCRLDHCCYETATQPLVEFKGSN