AlfalfaGEDB | MS.gene70008

M. sativa cultivar XinJiangDaYe / MS.gene70008

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF12796.10 PF13857.9 PF13962.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70008.t1	XP_003629044.1	85.3	449	62	2	1	447	1	447	8.00E-147	530.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70008.t1	Q8GYH5	48.7	302	146	3	11	305	4	303	5.7e-16	87.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70008.t1	G7L7W8	85.3	449	62	2	1	447	1	447	5.8e-147	530.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene33377	MS.gene70008	PPI
MS.gene70008	MS.gene038519	PPI
MS.gene70008	MS.gene27223	PPI
MS.gene71204	MS.gene70008	PPI
MS.gene007486	MS.gene70008	PPI
MS.gene041770	MS.gene70008	PPI
MS.gene70008	MS.gene050272	PPI
MS.gene26672	MS.gene70008	PPI
MS.gene97379	MS.gene70008	PPI
MS.gene70008	MS.gene050456	PPI
MS.gene058128	MS.gene70008	PPI
MS.gene70008	MS.gene51258	PPI
MS.gene70008	MS.gene32723	PPI
MS.gene066501	MS.gene70008	PPI
MS.gene25244	MS.gene70008	PPI
MS.gene70008	MS.gene053363	PPI
MS.gene08470	MS.gene70008	PPI
MS.gene63085	MS.gene70008	PPI
MS.gene056455	MS.gene70008	PPI
MS.gene89986	MS.gene70008	PPI
MS.gene26019	MS.gene70008	PPI
MS.gene70008	MS.gene32726	PPI
MS.gene70008	MS.gene71122	PPI
MS.gene035805	MS.gene70008	PPI
MS.gene70008	MS.gene73119	PPI
MS.gene70008	MS.gene92243	PPI
MS.gene70008	MS.gene85534	PPI
MS.gene69965	MS.gene70008	PPI
MS.gene066500	MS.gene70008	PPI
MS.gene70008	MS.gene51262	PPI
MS.gene70008	MS.gene23881	PPI
MS.gene70008	MS.gene014931	PPI
MS.gene82740	MS.gene70008	PPI
MS.gene70008	MS.gene062662	PPI
MS.gene70008	MS.gene21888	PPI
MS.gene70008	MS.gene066500	PPI
MS.gene70008	MS.gene39623	PPI
MS.gene30172	MS.gene70008	PPI
MS.gene70008	MS.gene90400	PPI
MS.gene23852	MS.gene70008	PPI
MS.gene69966	MS.gene70008	PPI
MS.gene97943	MS.gene70008	PPI
MS.gene071113	MS.gene70008	PPI
MS.gene027071	MS.gene70008	PPI
MS.gene67157	MS.gene70008	PPI
MS.gene70008	MS.gene81055	PPI
MS.gene30433	MS.gene70008	PPI
MS.gene70008	MS.gene65775	PPI
MS.gene012203	MS.gene70008	PPI
MS.gene70008	MS.gene80163	PPI
MS.gene022109	MS.gene70008	PPI
MS.gene34651	MS.gene70008	PPI
MS.gene70008	MS.gene02469	PPI
MS.gene010910	MS.gene70008	PPI
MS.gene025229	MS.gene70008	PPI
MS.gene70008	MS.gene045937	PPI
MS.gene70008	MS.gene027072	PPI
MS.gene063222	MS.gene70008	PPI
MS.gene25515	MS.gene70008	PPI
MS.gene07706	MS.gene70008	PPI
MS.gene027074	MS.gene70008	PPI
MS.gene70008	MS.gene51278	PPI
MS.gene70008	MS.gene87218	PPI
MS.gene69687	MS.gene70008	PPI
MS.gene010912	MS.gene70008	PPI
MS.gene25024	MS.gene70008	PPI
MS.gene23811	MS.gene70008	PPI
MS.gene23810	MS.gene70008	PPI
MS.gene70008	MS.gene043742	PPI
MS.gene70008	MS.gene027441	PPI
MS.gene024753	MS.gene70008	PPI
MS.gene78281	MS.gene70008	PPI
MS.gene005995	MS.gene70008	PPI
MS.gene037950	MS.gene70008	PPI
MS.gene70008	MS.gene30177	PPI
MS.gene70008	MS.gene008833	PPI
MS.gene068249	MS.gene70008	PPI
MS.gene051003	MS.gene70008	PPI
MS.gene64441	MS.gene70008	PPI
MS.gene24806	MS.gene70008	PPI
MS.gene004844	MS.gene70008	PPI
MS.gene30176	MS.gene70008	PPI
MS.gene70008	MS.gene48655	PPI
MS.gene70008	MS.gene09785	PPI
MS.gene007487	MS.gene70008	PPI
MS.gene018518	MS.gene70008	PPI
MS.gene066464	MS.gene70008	PPI
MS.gene70008	MS.gene65463	PPI
MS.gene70008	MS.gene007485	PPI
MS.gene51335	MS.gene70008	PPI
MS.gene70008	MS.gene037184	PPI
MS.gene24922	MS.gene70008	PPI
MS.gene027073	MS.gene70008	PPI
MS.gene70008	MS.gene23196	PPI
MS.gene064542	MS.gene70008	PPI
MS.gene47479	MS.gene70008	PPI
MS.gene70008	MS.gene067705	PPI
MS.gene008767	MS.gene70008	PPI
MS.gene34712	MS.gene70008	PPI
MS.gene70008	MS.gene050457	PPI
MS.gene70008	MS.gene99973	PPI
MS.gene70008	MS.gene30174	PPI
MS.gene36842	MS.gene70008	PPI
MS.gene70008	MS.gene26021	PPI
MS.gene99554	MS.gene70008	PPI
MS.gene066046	MS.gene70008	PPI
MS.gene99588	MS.gene70008	PPI
MS.gene027075	MS.gene70008	PPI
MS.gene41291	MS.gene70008	PPI
MS.gene70008	MS.gene91775	PPI
MS.gene27223	MS.gene70008	PPI
MS.gene70008	MS.gene050270	PPI
MS.gene052956	MS.gene70008	PPI
MS.gene70008	MS.gene052956	PPI
MS.gene70008	MS.gene60920	PPI
MS.gene22482	MS.gene70008	PPI
MS.gene039682	MS.gene70008	PPI
MS.gene061932	MS.gene70008	PPI
MS.gene70008	MS.gene34009	PPI
MS.gene70008	MS.gene043662	PPI
MS.gene70008	MS.gene065848	PPI
MS.gene26532	MS.gene70008	PPI
MS.gene042783	MS.gene70008	PPI
MS.gene70008	MS.gene99588	PPI
MS.gene066467	MS.gene70008	PPI
MS.gene70008	MS.gene54764	PPI
MS.gene21734	MS.gene70008	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70008.t1	MTR_8g072510	84.633	449	65	2	1	447	1	447	0.0	774
MS.gene70008.t1	MTR_8g062410	73.782	431	102	4	1	429	1	422	0.0	624
MS.gene70008.t1	MTR_7g021090	69.720	393	109	4	5	393	2	388	0.0	546
MS.gene70008.t1	MTR_3g016210	64.387	424	131	5	1	406	1	422	0.0	522
MS.gene70008.t1	MTR_8g039620	63.507	422	144	4	1	416	1	418	0.0	516
MS.gene70008.t1	MTR_8g069880	58.645	428	173	3	8	432	3	429	2.37e-163	469
MS.gene70008.t1	MTR_8g068990	62.133	375	123	4	1	371	1	360	1.39e-159	459
MS.gene70008.t1	MTR_1g059930	64.444	360	127	1	1	359	1	360	4.00e-158	454
MS.gene70008.t1	MTR_8g066670	57.208	437	182	3	1	436	1067	1499	3.20e-157	482
MS.gene70008.t1	MTR_8g073260	54.378	434	192	4	1	431	1	431	1.48e-152	441
MS.gene70008.t1	MTR_8g467780	54.545	429	179	5	11	423	10	438	4.89e-152	439
MS.gene70008.t1	MTR_7g451420	67.846	311	100	0	5	315	2	312	1.04e-147	423
MS.gene70008.t1	MTR_8g040930	58.177	373	149	3	54	420	1	372	3.59e-146	422
MS.gene70008.t1	MTR_8g041240	58.177	373	149	3	54	420	1	372	3.59e-146	422
MS.gene70008.t1	MTR_3g105650	44.390	410	193	5	10	410	3	386	4.51e-113	339
MS.gene70008.t1	MTR_8g073275	42.208	462	184	9	54	442	1	452	9.29e-104	316
MS.gene70008.t1	MTR_1g069110	22.958	453	295	11	15	440	21	446	1.91e-25	108
MS.gene70008.t1	MTR_7g099580	30.332	211	122	5	24	217	24	226	2.15e-20	92.4
MS.gene70008.t1	MTR_7g099570	29.187	209	134	3	12	215	9	208	6.61e-20	92.8
MS.gene70008.t1	MTR_2g101320	29.302	215	134	4	43	249	137	341	1.17e-18	89.0
MS.gene70008.t1	MTR_7g117620	25.436	287	180	5	47	311	119	393	1.70e-18	88.2
MS.gene70008.t1	MTR_2g096190	27.711	249	163	4	5	249	63	298	1.62e-17	85.5
MS.gene70008.t1	MTR_4g062100	23.780	328	199	8	15	311	77	384	2.40e-17	84.7
MS.gene70008.t1	MTR_2g101310	30.093	216	133	4	43	250	137	342	3.12e-17	84.7
MS.gene70008.t1	MTR_2g101310	29.167	216	135	3	43	250	137	342	6.80e-17	83.2
MS.gene70008.t1	MTR_1g115575	26.074	326	195	11	15	312	138	445	1.48e-16	82.4
MS.gene70008.t1	MTR_8g105260	24.483	290	182	6	47	311	101	378	4.81e-14	74.7
MS.gene70008.t1	MTR_1g032100	23.875	289	183	6	48	311	95	371	3.41e-13	72.0
MS.gene70008.t1	MTR_5g085500	31.847	157	95	4	81	233	8	156	4.79e-13	71.2
MS.gene70008.t1	MTR_7g100390	21.925	374	241	9	15	363	56	403	2.46e-12	69.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70008.t1	AT4G10720	38.235	442	237	7	11	437	4	424	5.41e-89	278
MS.gene70008.t1	AT4G10720	44.025	318	170	2	11	320	4	321	1.39e-84	265
MS.gene70008.t1	AT1G14500	35.484	403	223	6	11	399	4	383	8.68e-81	256
MS.gene70008.t1	AT1G14480	34.917	421	244	6	11	416	4	409	1.30e-79	253
MS.gene70008.t1	AT5G15500	37.197	371	213	5	12	373	6	365	8.75e-72	234
MS.gene70008.t1	AT5G54620	40.850	306	174	3	11	309	4	309	2.88e-70	229
MS.gene70008.t1	AT5G54610	37.954	303	179	3	11	306	4	304	6.63e-66	218
MS.gene70008.t1	AT1G14480	33.846	390	209	8	11	399	4	345	2.20e-62	207
MS.gene70008.t1	AT5G51160	28.796	382	228	10	66	417	35	402	1.40e-33	131
MS.gene70008.t1	AT5G15500	31.111	270	166	5	113	373	1	259	2.95e-29	117
MS.gene70008.t1	AT1G34050	25.177	282	169	6	56	315	162	423	3.70e-19	90.5
MS.gene70008.t1	AT5G54700	26.780	295	145	9	27	319	20	245	1.59e-18	88.2
MS.gene70008.t1	AT5G54700	30.172	232	116	8	104	327	23	216	2.18e-18	86.7
MS.gene70008.t1	AT2G01680	23.188	345	213	8	47	366	97	414	1.66e-17	85.1
MS.gene70008.t1	AT1G10340	28.814	236	128	7	112	315	201	428	4.71e-17	84.0
MS.gene70008.t1	AT1G10340	28.814	236	128	7	112	315	205	432	5.15e-17	84.0
MS.gene70008.t1	AT4G03460	29.577	213	115	5	111	312	298	486	5.23e-17	84.0
MS.gene70008.t1	AT4G03460	29.577	213	115	5	111	312	266	454	5.45e-17	84.0
MS.gene70008.t1	AT5G54710	27.899	276	160	10	68	315	187	451	8.56e-17	83.2
MS.gene70008.t1	AT4G03500	24.773	331	171	12	33	312	199	502	9.31e-17	83.2
MS.gene70008.t1	AT4G03500	24.773	331	171	12	33	312	176	479	1.06e-16	83.2
MS.gene70008.t1	AT4G03500	24.773	331	171	12	33	312	176	479	1.06e-16	83.2
MS.gene70008.t1	AT5G54710	27.899	276	160	10	68	315	232	496	1.09e-16	83.2
MS.gene70008.t1	AT1G07710	25.697	323	198	7	15	311	73	379	5.64e-16	80.5
MS.gene70008.t1	AT1G07710	25.697	323	198	7	15	311	73	379	5.64e-16	80.5
MS.gene70008.t1	AT1G05640	26.298	289	175	8	47	311	191	465	2.76e-15	78.6
MS.gene70008.t1	AT2G24600	25.938	320	190	9	33	315	123	432	3.16e-15	78.2
MS.gene70008.t1	AT2G24600	25.938	320	190	9	33	315	123	432	3.16e-15	78.2
MS.gene70008.t1	AT2G24600	25.938	320	190	9	33	315	123	432	3.71e-15	78.2
MS.gene70008.t1	AT2G24600	25.938	320	190	9	33	315	123	432	3.71e-15	78.2
MS.gene70008.t1	AT3G12360	24.679	389	198	13	4	312	58	431	1.17e-13	73.6
MS.gene70008.t1	AT3G12360	24.679	389	198	13	4	312	70	443	1.22e-13	73.6
MS.gene70008.t1	AT3G09550	24.390	328	198	9	15	312	150	457	7.56e-13	70.9
MS.gene70008.t1	AT3G09550	24.390	328	198	9	15	312	150	457	7.56e-13	70.9
MS.gene70008.t1	AT3G09550	24.390	328	198	9	15	312	157	464	8.19e-13	70.9
MS.gene70008.t1	AT4G03480	21.958	378	198	12	12	370	360	659	4.87e-12	68.6
MS.gene70008.t1	AT4G03480	21.958	378	198	12	12	370	357	656	5.21e-12	68.6
MS.gene70008.t1	AT2G31820	24.399	291	178	10	47	311	227	501	1.75e-11	66.6
MS.gene70008.t1	AT5G60070	23.548	310	213	10	4	311	103	390	7.64e-11	64.7
MS.gene70008.t1	AT5G50140	28.729	181	103	6	35	200	19	188	8.79e-11	64.3
MS.gene70008.t1	AT5G02620	22.156	334	220	7	5	311	53	373	9.70e-11	64.3
MS.gene70008.t1	AT5G02620	22.156	334	220	7	5	311	53	373	9.70e-11	64.3
MS.gene70008.t1	AT5G02620	22.156	334	220	7	5	311	53	373	9.70e-11	64.3

Find 83 sgRNAs with CRISPR-Local

Find 117 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGACCTTGCTAGATTTAG+AGG	0.243155	8.1:-33088690	MS.gene70008:CDS
TGTTAGCTTACTTTCTCTAT+TGG	0.282178	8.1:-33088614	MS.gene70008:CDS
TCAAGATGATCCATACGTTT+TGG	0.300125	8.1:-33088910	MS.gene70008:CDS
AGTGATACACCATACGTTCT+TGG	0.312014	8.1:+33088727	None:intergenic
GTCAAGTATTCAATGGATTC+TGG	0.313131	8.1:+33088588	None:intergenic
AGATCAGAGATCACAATTAT+AGG	0.314909	8.1:-33088042	MS.gene70008:CDS
AACACCACAAACACAAATAA+TGG	0.318375	8.1:-33088990	MS.gene70008:CDS
GTTCATTCTTAACAGCAATA+TGG	0.326006	8.1:+33088544	None:intergenic
TAGATTTGAAGGCAAAGAAT+AGG	0.327050	8.1:-33088182	MS.gene70008:CDS
TGGGGTAGAGATGGTAAAAT+TGG	0.332925	8.1:+33087846	None:intergenic
ACTGATTTCCCAGCATTTCC+TGG	0.346230	8.1:+33087826	None:intergenic
TCTTATTATATGGTCAAATC+TGG	0.347724	8.1:+33088062	None:intergenic
ATCTCCATTATTTGTGTTTG+TGG	0.350305	8.1:+33088986	None:intergenic
AGTCAAAGCATTGTTTCAAT+AGG	0.352470	8.1:-33087506	None:intergenic
TGATCTCTGATCTTATTATA+TGG	0.363350	8.1:+33088052	None:intergenic
ATCTCTACCCCAGGAAATGC+TGG	0.372886	8.1:-33087835	MS.gene70008:CDS
TTTACATATTGCTGCATCCA+TGG	0.394377	8.1:-33088853	MS.gene70008:CDS
TTTCAAATTCATAATTTCAA+TGG	0.401833	8.1:+33088815	None:intergenic
TGGTCTAGTGGCTTCTCCAA+TGG	0.414120	8.1:-33087701	MS.gene70008:CDS
CCCTTCAAGTACTTGTTAGC+TGG	0.417708	8.1:-33088512	MS.gene70008:CDS
CAGTGCTGAAATATGCAAAA+TGG	0.438183	8.1:+33088413	None:intergenic
ATGATACCATATGGAATACT+AGG	0.438251	8.1:-33087724	MS.gene70008:CDS
GGTCTAGTGGCTTCTCCAAT+GGG	0.445312	8.1:-33087700	MS.gene70008:CDS
TGTAAAGGAGTTTCAACAAA+TGG	0.448723	8.1:+33088870	None:intergenic
TTTAGCTTCAAAGCAAATGA+AGG	0.453815	8.1:+33088792	None:intergenic
AGATTTGAAGGCAAAGAATA+GGG	0.462237	8.1:-33088181	MS.gene70008:CDS
TTCTGCATAGCGAGATGGAT+GGG	0.464517	8.1:+33088753	None:intergenic
CGCTAAAATTGATAAAGAAA+AGG	0.465233	8.1:+33087756	None:intergenic
GCAACTGCCATCTATCAATC+AGG	0.470824	8.1:-33087934	MS.gene70008:CDS
ATTCAATCCTGATTGATAGA+TGG	0.475227	8.1:+33087927	None:intergenic
CTGATTTCCCAGCATTTCCT+GGG	0.478660	8.1:+33087827	None:intergenic
TTGAAAGCTGCAGCTGAAGC+AGG	0.480701	8.1:-33088960	MS.gene70008:CDS
TAGTGGCTTCTCCAATGGGT+TGG	0.487019	8.1:-33087696	MS.gene70008:CDS
GCCAGCTAACAAGTACTTGA+AGG	0.489142	8.1:+33088511	None:intergenic
TGGTTCAATTCTGTTAGCCA+TGG	0.493632	8.1:-33087596	MS.gene70008:CDS
AATAATATACTCTTGATCTC+TGG	0.497884	8.1:+33088126	None:intergenic
AACACAGGCAGTTCTATTGT+TGG	0.498997	8.1:-33088217	MS.gene70008:intron
AAGTACTAGTATAGATTTGA+AGG	0.503292	8.1:-33088193	MS.gene70008:CDS
TCACGGAATTGACATGTGTA+GGG	0.506012	8.1:+33087635	None:intergenic
CCTCACAGTCAAGTATTCAA+TGG	0.507786	8.1:+33088581	None:intergenic
TGTTAAGAATGAACAATATG+AGG	0.510561	8.1:-33088535	MS.gene70008:CDS
TTACATATTGCTGCATCCAT+GGG	0.527095	8.1:-33088852	MS.gene70008:CDS
AAGAGTATATTATTAAGTGT+TGG	0.527914	8.1:-33088117	MS.gene70008:CDS
TCTATACGCTCCAAAACGTA+TGG	0.533250	8.1:+33088900	None:intergenic
GCTGGGAAATCAGTCTTGCC+AGG	0.536127	8.1:-33087817	MS.gene70008:CDS
CTTATTATATGGTCAAATCT+GGG	0.539755	8.1:+33088063	None:intergenic
GTTCTGCATAGCGAGATGGA+TGG	0.540228	8.1:+33088752	None:intergenic
ATTTGACTTGCAAAATGAAG+AGG	0.542519	8.1:+33088651	None:intergenic
TCATTTATGCAGTGTACGCA+AGG	0.545857	8.1:-33087564	MS.gene70008:CDS
AGACCACCTAGTATTCCATA+TGG	0.551321	8.1:+33087718	None:intergenic
GGTAGAGATGGTAAAATTGG+AGG	0.552649	8.1:+33087849	None:intergenic
AATATTAAACAAGATAGATA+AGG	0.553024	8.1:-33088445	MS.gene70008:CDS
CAGAGAAGCAACGTAACACT+TGG	0.554788	8.1:-33087978	MS.gene70008:CDS
TCATCAGTGACTTCTGAGCT+AGG	0.554883	8.1:+33088087	None:intergenic
AGCACTGAGCAGTGACCCGC+AGG	0.557387	8.1:-33088397	MS.gene70008:intron
GCTAGATTTAGAGGAAGAGA+AGG	0.558608	8.1:-33088681	MS.gene70008:CDS
AGCATTTCCTGGGGTAGAGA+TGG	0.561933	8.1:+33087837	None:intergenic
ATGGATGCAGCAATATGTAA+AGG	0.578412	8.1:+33088855	None:intergenic
CTCTTCCTCTAAATCTAGCA+AGG	0.582930	8.1:+33088685	None:intergenic
GCAGAACAACCAAGAACGTA+TGG	0.591744	8.1:-33088736	MS.gene70008:CDS
TTAAACAAGATAGATAAGGC+AGG	0.593894	8.1:-33088441	MS.gene70008:CDS
CTAACCTGATAAATTCCACC+AGG	0.595881	8.1:+33087901	None:intergenic
TCTCTACCCCAGGAAATGCT+GGG	0.602386	8.1:-33087834	MS.gene70008:CDS
TGGAATACTAGGTGGTCTAG+TGG	0.604271	8.1:-33087713	MS.gene70008:CDS
GGCAAAGTCGATATGACCCA+TGG	0.604662	8.1:+33088836	None:intergenic
GGTTGTTCTGCATAGCGAGA+TGG	0.604900	8.1:+33088748	None:intergenic
CCAGCTAACAAGTACTTGAA+GGG	0.613435	8.1:+33088512	None:intergenic
ATCACGGAATTGACATGTGT+AGG	0.614865	8.1:+33087634	None:intergenic
CTAGCGGTAAACCAACCCAT+TGG	0.623589	8.1:+33087685	None:intergenic
GATCACAATTATAGGTGCAG+TGG	0.629086	8.1:-33088034	MS.gene70008:CDS
TCTGCATAGCGAGATGGATG+GGG	0.636628	8.1:+33088754	None:intergenic
CATCTTGAATTACTGCGTAG+AGG	0.646104	8.1:+33088925	None:intergenic
CACGGAATTGACATGTGTAG+GGG	0.649635	8.1:+33087636	None:intergenic
GCTTTGAAGCTAAATCCGCA+AGG	0.665685	8.1:-33088783	MS.gene70008:CDS
TGGATGGGGCTGAATCCTTG+CGG	0.668802	8.1:+33088768	None:intergenic
CATAGAAAATAAATAACTAG+CGG	0.673714	8.1:+33087669	None:intergenic
CCATTGAATACTTGACTGTG+AGG	0.682234	8.1:-33088581	MS.gene70008:CDS
TGATTTCCCAGCATTTCCTG+GGG	0.683910	8.1:+33087828	None:intergenic
AACCTGATAAATTCCACCAG+GGG	0.693447	8.1:+33087903	None:intergenic
TAACCTGATAAATTCCACCA+GGG	0.695982	8.1:+33087902	None:intergenic
ATAAATGACGTCAATCACCA+TGG	0.702124	8.1:+33087579	None:intergenic
ACCTGATAAATTCCACCAGG+GGG	0.708869	8.1:+33087904	None:intergenic
ATACCATATGGAATACTAGG+TGG	0.714096	8.1:-33087721	MS.gene70008:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATATTAAACAAGATAGATA+AGG	-	chr8.1:33088055-33088074	MS.gene70008:CDS	15.0%
!!	TGTATTATTCATTAATTTCT+TGG	-	chr8.1:33088259-33088278	MS.gene70008:intron	15.0%
!!	TTTCAAATTCATAATTTCAA+TGG	+	chr8.1:33087688-33087707	None:intergenic	15.0%
!!!	ATATATTTTATGATACCATA+TGG	-	chr8.1:33088767-33088786	MS.gene70008:CDS	15.0%
!!!	TCTATATCTTATTTATAAGT+TGG	+	chr8.1:33088211-33088230	None:intergenic	15.0%
!!	AAAAATGATGAATACAATCA+CGG	+	chr8.1:33088885-33088904	None:intergenic	20.0%
!!	CATAGAAAATAAATAACTAG+CGG	+	chr8.1:33088834-33088853	None:intergenic	20.0%
!!!	AAGAGTATATTATTAAGTGT+TGG	-	chr8.1:33088383-33088402	MS.gene70008:intron	20.0%
!!!	ATCTTATTTATAAGTTGGTA+GGG	+	chr8.1:33088206-33088225	None:intergenic	20.0%
!!!	CTAGTTATTTATTTTCTATG+TGG	-	chr8.1:33088834-33088853	MS.gene70008:CDS	20.0%
!!!	TATCTTATTTATAAGTTGGT+AGG	+	chr8.1:33088207-33088226	None:intergenic	20.0%
!	AACAAAAAGAATTCATATCC+TGG	+	chr8.1:33088704-33088723	None:intergenic	25.0%
!	AAGTACTAGTATAGATTTGA+AGG	-	chr8.1:33088307-33088326	MS.gene70008:intron	25.0%
!	AATAATATACTCTTGATCTC+TGG	+	chr8.1:33088377-33088396	None:intergenic	25.0%
!	CGCTAAAATTGATAAAGAAA+AGG	+	chr8.1:33088747-33088766	None:intergenic	25.0%
!	CTTATTATATGGTCAAATCT+GGG	+	chr8.1:33088440-33088459	None:intergenic	25.0%
!	TCTTATTATATGGTCAAATC+TGG	+	chr8.1:33088441-33088460	None:intergenic	25.0%
!	TGATCTCTGATCTTATTATA+TGG	+	chr8.1:33088451-33088470	None:intergenic	25.0%
!	TGTTAAGAATGAACAATATG+AGG	-	chr8.1:33087965-33087984	MS.gene70008:CDS	25.0%
!!	GTGATTGTATTCATCATTTT+TGG	-	chr8.1:33088884-33088903	MS.gene70008:CDS	25.0%
!!!	GTTATTTATTTTCTATGTGG+AGG	-	chr8.1:33088837-33088856	MS.gene70008:CDS	25.0%
!!!	TCTTATTTATAAGTTGGTAG+GGG	+	chr8.1:33088205-33088224	None:intergenic	25.0%
	AACACCACAAACACAAATAA+TGG	-	chr8.1:33087510-33087529	MS.gene70008:CDS	30.0%
	AGATCAGAGATCACAATTAT+AGG	-	chr8.1:33088458-33088477	MS.gene70008:CDS	30.0%
	AGATTTGAAGGCAAAGAATA+GGG	-	chr8.1:33088319-33088338	MS.gene70008:intron	30.0%
	ATCTCCATTATTTGTGTTTG+TGG	+	chr8.1:33087517-33087536	None:intergenic	30.0%
	ATGATACCATATGGAATACT+AGG	-	chr8.1:33088776-33088795	MS.gene70008:CDS	30.0%
	ATTCAATCCTGATTGATAGA+TGG	+	chr8.1:33088576-33088595	None:intergenic	30.0%
	GTTCATTCTTAACAGCAATA+TGG	+	chr8.1:33087959-33087978	None:intergenic	30.0%
	TAGATTTGAAGGCAAAGAAT+AGG	-	chr8.1:33088318-33088337	MS.gene70008:intron	30.0%
	TCAAGAAAAATACACAGAGA+GGG	-	chr8.1:33088012-33088031	MS.gene70008:CDS	30.0%
	TGTAAAGGAGTTTCAACAAA+TGG	+	chr8.1:33087633-33087652	None:intergenic	30.0%
	TTAAACAAGATAGATAAGGC+AGG	-	chr8.1:33088059-33088078	MS.gene70008:CDS	30.0%
!	CATTAATTTCTTGGTAACAC+AGG	-	chr8.1:33088268-33088287	MS.gene70008:intron	30.0%
!	CTTCATTTTGCAAGTCAAAT+TGG	-	chr8.1:33087852-33087871	MS.gene70008:CDS	30.0%
!	TGTTAGCTTACTTTCTCTAT+TGG	-	chr8.1:33087886-33087905	MS.gene70008:CDS	30.0%
!	TTTAGCTTCAAAGCAAATGA+AGG	+	chr8.1:33087711-33087730	None:intergenic	30.0%
!!	ATTTGACTTGCAAAATGAAG+AGG	+	chr8.1:33087852-33087871	None:intergenic	30.0%
!!	GAGACATGTGATTTTAGTAA+TGG	+	chr8.1:33088145-33088164	None:intergenic	30.0%
	AAAGACCTTGCTAGATTTAG+AGG	-	chr8.1:33087810-33087829	MS.gene70008:CDS	35.0%
	AGGCGACAGGAAATATATAA+AGG	+	chr8.1:33088167-33088186	None:intergenic	35.0%
	ATAAATGACGTCAATCACCA+TGG	+	chr8.1:33088924-33088943	None:intergenic	35.0%
	ATACCATATGGAATACTAGG+TGG	-	chr8.1:33088779-33088798	MS.gene70008:CDS	35.0%
	ATGAGAATAAGATTACCTGC+GGG	+	chr8.1:33088121-33088140	None:intergenic	35.0%
	ATGGATGCAGCAATATGTAA+AGG	+	chr8.1:33087648-33087667	None:intergenic	35.0%
	CAAGAAAAATACACAGAGAG+GGG	-	chr8.1:33088013-33088032	MS.gene70008:CDS	35.0%
	CATGAGAATAAGATTACCTG+CGG	+	chr8.1:33088122-33088141	None:intergenic	35.0%
	CTCAAGAAAAATACACAGAG+AGG	-	chr8.1:33088011-33088030	MS.gene70008:CDS	35.0%
	TAACCTGATAAATTCCACCA+GGG	+	chr8.1:33088601-33088620	None:intergenic	35.0%
	TTACATATTGCTGCATCCAT+GGG	-	chr8.1:33087648-33087667	MS.gene70008:CDS	35.0%
	TTTACATATTGCTGCATCCA+TGG	-	chr8.1:33087647-33087666	MS.gene70008:CDS	35.0%
!	CAGTGCTGAAATATGCAAAA+TGG	+	chr8.1:33088090-33088109	None:intergenic	35.0%
!	GTCAAGTATTCAATGGATTC+TGG	+	chr8.1:33087915-33087934	None:intergenic	35.0%
!	TCAAGATGATCCATACGTTT+TGG	-	chr8.1:33087590-33087609	MS.gene70008:CDS	35.0%
!	TTTTGCAAGTCAAATTGGTG+AGG	-	chr8.1:33087857-33087876	MS.gene70008:CDS	35.0%
	AACACAGGCAGTTCTATTGT+TGG	-	chr8.1:33088283-33088302	MS.gene70008:intron	40.0%
	AACCTGATAAATTCCACCAG+GGG	+	chr8.1:33088600-33088619	None:intergenic	40.0%
	AGACCACCTAGTATTCCATA+TGG	+	chr8.1:33088785-33088804	None:intergenic	40.0%
	AGTGATACACCATACGTTCT+TGG	+	chr8.1:33087776-33087795	None:intergenic	40.0%
	ATCACGGAATTGACATGTGT+AGG	+	chr8.1:33088869-33088888	None:intergenic	40.0%
	CATCTTGAATTACTGCGTAG+AGG	+	chr8.1:33087578-33087597	None:intergenic	40.0%
	CCAGCTAACAAGTACTTGAA+GGG	+	chr8.1:33087991-33088010	None:intergenic	40.0%
	CCATTGAATACTTGACTGTG+AGG	-	chr8.1:33087919-33087938	MS.gene70008:CDS	40.0%
	CCTCACAGTCAAGTATTCAA+TGG	+	chr8.1:33087922-33087941	None:intergenic	40.0%
	CTAACCTGATAAATTCCACC+AGG	+	chr8.1:33088602-33088621	None:intergenic	40.0%
	CTCTTCCTCTAAATCTAGCA+AGG	+	chr8.1:33087818-33087837	None:intergenic	40.0%
	GATCACAATTATAGGTGCAG+TGG	-	chr8.1:33088466-33088485	MS.gene70008:CDS	40.0%
	GCTAGATTTAGAGGAAGAGA+AGG	-	chr8.1:33087819-33087838	MS.gene70008:CDS	40.0%
	GGTAGAGATGGTAAAATTGG+AGG	+	chr8.1:33088654-33088673	None:intergenic	40.0%
	TCACGGAATTGACATGTGTA+GGG	+	chr8.1:33088868-33088887	None:intergenic	40.0%
	TCATTTATGCAGTGTACGCA+AGG	-	chr8.1:33088936-33088955	MS.gene70008:CDS	40.0%
	TCTATACGCTCCAAAACGTA+TGG	+	chr8.1:33087603-33087622	None:intergenic	40.0%
	TGGGGTAGAGATGGTAAAAT+TGG	+	chr8.1:33088657-33088676	None:intergenic	40.0%
	TGGTTCAATTCTGTTAGCCA+TGG	-	chr8.1:33088904-33088923	MS.gene70008:CDS	40.0%
!	AATTTTACCATCTCTACCCC+AGG	-	chr8.1:33088656-33088675	MS.gene70008:CDS	40.0%
!!	TTTATAAGTTGGTAGGGGAG+CGG	+	chr8.1:33088200-33088219	None:intergenic	40.0%
	ACCTGATAAATTCCACCAGG+GGG	+	chr8.1:33088599-33088618	None:intergenic	45.0%
	CACGGAATTGACATGTGTAG+GGG	+	chr8.1:33088867-33088886	None:intergenic	45.0%
	CAGAGAAGCAACGTAACACT+TGG	-	chr8.1:33088522-33088541	MS.gene70008:CDS	45.0%
	CTGATTTCCCAGCATTTCCT+GGG	+	chr8.1:33088676-33088695	None:intergenic	45.0%
	GCAACTGCCATCTATCAATC+AGG	-	chr8.1:33088566-33088585	MS.gene70008:CDS	45.0%
	GCAGAACAACCAAGAACGTA+TGG	-	chr8.1:33087764-33087783	MS.gene70008:CDS	45.0%
	GCCAGCTAACAAGTACTTGA+AGG	+	chr8.1:33087992-33088011	None:intergenic	45.0%
	TCATCAGTGACTTCTGAGCT+AGG	+	chr8.1:33088416-33088435	None:intergenic	45.0%
	TGATTTCCCAGCATTTCCTG+GGG	+	chr8.1:33088675-33088694	None:intergenic	45.0%
	TGGAATACTAGGTGGTCTAG+TGG	-	chr8.1:33088787-33088806	MS.gene70008:CDS	45.0%
	TTCTGCATAGCGAGATGGAT+GGG	+	chr8.1:33087750-33087769	None:intergenic	45.0%
!	ACTGATTTCCCAGCATTTCC+TGG	+	chr8.1:33088677-33088696	None:intergenic	45.0%
!	CCCTTCAAGTACTTGTTAGC+TGG	-	chr8.1:33087988-33088007	MS.gene70008:CDS	45.0%
!!	GCTTTGAAGCTAAATCCGCA+AGG	-	chr8.1:33087717-33087736	MS.gene70008:CDS	45.0%
	ACAGAGAGGGGCAAAAAAGC+TGG	-	chr8.1:33088025-33088044	MS.gene70008:CDS	50.0%
	AGCATTTCCTGGGGTAGAGA+TGG	+	chr8.1:33088666-33088685	None:intergenic	50.0%
	ATCTCTACCCCAGGAAATGC+TGG	-	chr8.1:33088665-33088684	MS.gene70008:CDS	50.0%
	CAATCAGGATTGAATCCCCC+TGG	-	chr8.1:33088581-33088600	MS.gene70008:CDS	50.0%
	CTAGCGGTAAACCAACCCAT+TGG	+	chr8.1:33088818-33088837	None:intergenic	50.0%
	GGCAAAGTCGATATGACCCA+TGG	+	chr8.1:33087667-33087686	None:intergenic	50.0%
	GGTCTAGTGGCTTCTCCAAT+GGG	-	chr8.1:33088800-33088819	MS.gene70008:CDS	50.0%
	GGTTGTTCTGCATAGCGAGA+TGG	+	chr8.1:33087755-33087774	None:intergenic	50.0%
	GTTCTGCATAGCGAGATGGA+TGG	+	chr8.1:33087751-33087770	None:intergenic	50.0%
	TAGTGGCTTCTCCAATGGGT+TGG	-	chr8.1:33088804-33088823	MS.gene70008:CDS	50.0%
	TCCCCCTGGTGGAATTTATC+AGG	-	chr8.1:33088595-33088614	MS.gene70008:CDS	50.0%
	TCTCTACCCCAGGAAATGCT+GGG	-	chr8.1:33088666-33088685	MS.gene70008:CDS	50.0%
	TCTGCATAGCGAGATGGATG+GGG	+	chr8.1:33087749-33087768	None:intergenic	50.0%
	TGGTCTAGTGGCTTCTCCAA+TGG	-	chr8.1:33088799-33088818	MS.gene70008:CDS	50.0%
	TTGAAAGCTGCAGCTGAAGC+AGG	-	chr8.1:33087540-33087559	MS.gene70008:CDS	50.0%
	GCTGGGAAATCAGTCTTGCC+AGG	-	chr8.1:33088683-33088702	MS.gene70008:CDS	55.0%
	TCAGGATTGAATCCCCCTGG+TGG	-	chr8.1:33088584-33088603	MS.gene70008:CDS	55.0%
	TGGATGGGGCTGAATCCTTG+CGG	+	chr8.1:33087735-33087754	None:intergenic	55.0%
!!	ATAAGTTGGTAGGGGAGCGG+AGG	+	chr8.1:33088197-33088216	None:intergenic	55.0%
!!	TAAGTTGGTAGGGGAGCGGA+GGG	+	chr8.1:33088196-33088215	None:intergenic	55.0%
!!	AAGTTGGTAGGGGAGCGGAG+GGG	+	chr8.1:33088195-33088214	None:intergenic	60.0%
!	AGCACTGAGCAGTGACCCGC+AGG	-	chr8.1:33088103-33088122	MS.gene70008:CDS	65.0%
	TGGTAGGGGAGCGGAGGGGA+GGG	+	chr8.1:33088191-33088210	None:intergenic	70.0%
	TTGGTAGGGGAGCGGAGGGG+AGG	+	chr8.1:33088192-33088211	None:intergenic	70.0%
	GGTAGGGGAGCGGAGGGGAG+GGG	+	chr8.1:33088190-33088209	None:intergenic	75.0%
!	AGGGGAGCGGAGGGGAGGGG+AGG	+	chr8.1:33088187-33088206	None:intergenic	80.0%
!	CGGAGGGGAGGGGAGGCGAC+AGG	+	chr8.1:33088180-33088199	None:intergenic	80.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.1	gene	33087507	33089015	33087507	ID=MS.gene70008
chr8.1	mRNA	33087507	33089015	33087507	ID=MS.gene70008.t1;Parent=MS.gene70008
chr8.1	exon	33088398	33089015	33088398	ID=MS.gene70008.t1.exon1;Parent=MS.gene70008.t1
chr8.1	CDS	33088398	33089015	33088398	ID=cds.MS.gene70008.t1;Parent=MS.gene70008.t1
chr8.1	exon	33087507	33088232	33087507	ID=MS.gene70008.t1.exon2;Parent=MS.gene70008.t1
chr8.1	CDS	33087507	33088232	33087507	ID=cds.MS.gene70008.t1;Parent=MS.gene70008.t1

Gene Sequence

>MS.gene70008

ATGAACACCACAAACACAAATAATGGAGATCGATTGAAAGCTGCAGCTGAAGCAGGTGATATAGATCTCCTCTACGCAGTAATTCAAGATGATCCATACGTTTTGGAGCGTATAGATTCAATACCATTTGTTGAAACTCCTTTACATATTGCTGCATCCATGGGTCATATCGACTTTGCCATTGAAATTATGAATTTGAAACCTTCATTTGCTTTGAAGCTAAATCCGCAAGGATTCAGCCCCATCCATCTCGCTATGCAGAACAACCAAGAACGTATGGTGTATCACTATGTGTTTGTCAATAAAGACCTTGCTAGATTTAGAGGAAGAGAAGGCATAACTCCTCTTCATTTTGCAAGTCAAATTGGTGAGGTTGAAATGTTAGCTTACTTTCTCTATTGGTGTCCAGAATCCATTGAATACTTGACTGTGAGGCGCGAGACTGCACTCCATATTGCTGTTAAGAATGAACAATATGAGGCCCTTCAAGTACTTGTTAGCTGGCTCAAGAAAAATACACAGAGAGGGGCAAAAAAGCTGGAAAATGAAATATTAAACAAGATAGATAAGGCAGGAAACACCATTTTGCATATTTCAGCACTGAGCAGTGACCCGCAGGTAATCTTATTCTCATGCCATTACTAAAATCACATGTCTCCTTTATATATTTCCTGTCGCCTCCCCTCCCCTCCGCTCCCCTACCAACTTATAAATAAGATATAGAATTGCTAACATGAATGATCTCTAACAATTGTATTATTCATTAATTTCTTGGTAACACAGGCAGTTCTATTGTTGGTAAGTACTAGTATAGATTTGAAGGCAAAGAATAGGGAGAACAAAACTGCATTAGACATAGCAAGCATACCAGAGATCAAGAGTATATTATTAAGTGTTGGAGCAAAACCTAGCTCAGAAGTCACTGATGATCCCAGATTTGACCATATAATAAGATCAGAGATCACAATTATAGGTGCAGTGGCAATTTATATACATCGCATTAGAAGAGATATATCAGAGAAGCAACGTAACACTTGGTTGATAATTGCGACTCTTGTTGCAACTGCCATCTATCAATCAGGATTGAATCCCCCTGGTGGAATTTATCAGGTTAGTGCTAGTGACACTAACGTGAACATCACTTCCTCCAATTTTACCATCTCTACCCCAGGAAATGCTGGGAAATCAGTCTTGCCAGGATATGAATTCTTTTTGTTTTTATTTGTAAATATGTATTCCTTTTCTTTATCAATTTTAGCGATATATTTTATGATACCATATGGAATACTAGGTGGTCTAGTGGCTTCTCCAATGGGTTGGTTTACCGCTAGTTATTTATTTTCTATGTGGAGGATATCCCCTACACATGTCAATTCCGTGATTGTATTCATCATTTTTGGTTCAATTCTGTTAGCCATGGTGATTGACGTCATTTATGCAGTGTACGCAAGGCTTAAACGCAAAACTGCTAAGATATTTAATATGATAGTCAAAGCATTGTTTCAATAG

Protein sequence

>MS.gene70008.t1

MNTTNTNNGDRLKAAAEAGDIDLLYAVIQDDPYVLERIDSIPFVETPLHIAASMGHIDFAIEIMNLKPSFALKLNPQGFSPIHLAMQNNQERMVYHYVFVNKDLARFRGREGITPLHFASQIGEVEMLAYFLYWCPESIEYLTVRRETALHIAVKNEQYEALQVLVSWLKKNTQRGAKKLENEILNKIDKAGNTILHISALSSDPQAVLLLVSTSIDLKAKNRENKTALDIASIPEIKSILLSVGAKPSSEVTDDPRFDHIIRSEITIIGAVAIYIHRIRRDISEKQRNTWLIIATLVATAIYQSGLNPPGGIYQVSASDTNVNITSSNFTISTPGNAGKSVLPGYEFFLFLFVNMYSFSLSILAIYFMIPYGILGGLVASPMGWFTASYLFSMWRISPTHVNSVIVFIIFGSILLAMVIDVIYAVYARLKRKTAKIFNMIVKALFQ