AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene63085

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63085.t1 MTR_8g062410 85.879 347 26 2 1 331 1 340 0.0 575
MS.gene63085.t1 MTR_8g072510 72.543 346 79 2 1 331 1 345 9.14e-173 490
MS.gene63085.t1 MTR_7g021090 69.733 337 81 3 4 325 3 333 3.55e-162 462
MS.gene63085.t1 MTR_8g039620 69.591 342 85 3 2 325 6 346 2.43e-161 461
MS.gene63085.t1 MTR_3g016210 64.507 355 95 3 1 325 1 354 2.11e-157 451
MS.gene63085.t1 MTR_1g059930 63.663 344 104 3 1 325 1 342 1.24e-145 421
MS.gene63085.t1 MTR_8g068990 60.819 342 101 4 2 325 4 330 1.18e-138 404
MS.gene63085.t1 MTR_7g451420 63.987 311 97 1 3 298 2 312 1.69e-135 390
MS.gene63085.t1 MTR_8g069880 61.310 336 112 3 6 325 3 336 1.07e-130 384
MS.gene63085.t1 MTR_8g073260 56.772 347 131 4 1 331 1 344 3.30e-130 382
MS.gene63085.t1 MTR_8g040930 63.014 292 89 2 52 325 1 291 1.06e-122 360
MS.gene63085.t1 MTR_8g041240 63.014 292 89 2 52 325 1 291 1.06e-122 360
MS.gene63085.t1 MTR_8g066670 60.519 347 117 5 1 331 1067 1409 1.48e-120 382
MS.gene63085.t1 MTR_8g467780 54.678 342 130 2 9 325 10 351 4.92e-113 338
MS.gene63085.t1 MTR_3g105650 49.355 310 134 3 8 295 3 311 2.36e-94 289
MS.gene63085.t1 MTR_8g073275 38.978 372 151 8 52 350 1 369 1.97e-65 216
MS.gene63085.t1 MTR_1g069110 27.619 315 201 9 10 311 18 318 7.65e-25 105
MS.gene63085.t1 MTR_7g099580 27.046 281 171 6 22 298 24 274 1.11e-22 98.2
MS.gene63085.t1 MTR_7g099570 29.592 196 124 3 10 200 9 195 4.65e-19 89.7
MS.gene63085.t1 MTR_2g101320 28.986 207 134 3 41 234 137 343 2.66e-16 81.3
MS.gene63085.t1 MTR_1g115575 25.959 339 202 12 10 314 135 458 4.99e-16 80.1
MS.gene63085.t1 MTR_2g101310 27.273 209 139 3 37 232 133 341 1.05e-14 75.9
MS.gene63085.t1 MTR_2g101310 27.273 209 139 3 37 232 133 341 1.11e-14 76.3
MS.gene63085.t1 MTR_7g117620 24.476 286 167 7 45 293 119 392 2.29e-12 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63085.t1 AT4G10720 39.375 320 171 3 9 305 4 323 4.15e-72 231
MS.gene63085.t1 AT4G10720 39.375 320 171 3 9 305 4 323 1.33e-71 231
MS.gene63085.t1 AT1G14480 36.842 323 179 2 9 306 4 326 3.65e-66 217
MS.gene63085.t1 AT1G14500 36.943 314 174 2 9 298 4 317 1.20e-65 216
MS.gene63085.t1 AT5G15500 39.688 320 165 6 10 306 6 320 3.37e-62 207
MS.gene63085.t1 AT5G54620 39.216 306 164 3 9 292 4 309 2.12e-60 202
MS.gene63085.t1 AT5G54610 35.314 303 172 4 9 289 4 304 1.63e-54 186
MS.gene63085.t1 AT1G14480 36.242 298 172 3 9 306 4 283 5.71e-54 184
MS.gene63085.t1 AT5G15500 33.790 219 117 6 111 306 1 214 5.06e-24 102
MS.gene63085.t1 AT5G51160 28.727 275 151 8 64 301 35 301 5.39e-23 100
MS.gene63085.t1 AT5G54700 26.690 281 148 10 25 302 20 245 4.39e-17 83.2
MS.gene63085.t1 AT5G50140 29.885 174 97 4 40 198 25 188 8.44e-13 70.1
MS.gene63085.t1 AT4G03490 26.129 310 152 11 10 295 211 467 9.21e-13 70.5
MS.gene63085.t1 AT4G03490 26.129 310 152 11 10 295 211 467 1.12e-12 70.1
MS.gene63085.t1 AT4G03490 26.129 310 152 11 10 295 201 457 1.13e-12 70.1
MS.gene63085.t1 AT4G03500 25.311 241 149 5 72 295 276 502 1.42e-12 69.7
MS.gene63085.t1 AT4G03500 25.311 241 149 5 72 295 253 479 1.59e-12 69.7
MS.gene63085.t1 AT4G03500 25.311 241 149 5 72 295 253 479 1.59e-12 69.7
MS.gene63085.t1 AT5G54700 25.221 226 116 7 96 302 17 208 1.57e-11 65.5
MS.gene63085.t1 AT3G12360 24.658 292 164 7 45 295 167 443 1.88e-11 66.2
MS.gene63085.t1 AT3G09550 24.107 336 202 12 10 309 147 465 2.49e-11 65.9
MS.gene63085.t1 AT3G09550 24.107 336 202 12 10 309 147 465 2.49e-11 65.9
MS.gene63085.t1 AT3G12360 24.658 292 164 7 45 295 155 431 2.62e-11 65.9
MS.gene63085.t1 AT3G09550 24.107 336 202 12 10 309 154 472 2.67e-11 65.9
MS.gene63085.t1 AT4G03490 26.298 289 140 9 10 295 201 419 2.81e-11 65.9
MS.gene63085.t1 AT4G03490 25.606 289 142 9 10 295 191 409 2.90e-11 65.9

Find 89 sgRNAs with CRISPR-Local

Find 195 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAGACCTTGTTAGAATTAA+AGG 0.120242 8.1:-42790391 MS.gene63085:CDS
TTTACATATTGCTGCATCTT+TGG 0.150272 8.1:-42790554 MS.gene63085:CDS
TCAAGATGATCCATCCATTT+TGG 0.183666 8.1:-42790611 MS.gene63085:CDS
CTCTTCAATTACTTGTTATC+TGG 0.238879 8.1:-42790213 MS.gene63085:CDS
CGTGAATCCTTTCCTGCAAT+TGG 0.295174 8.1:+42788124 None:intergenic
AAGAGTATATTATTAAGTTT+TGG 0.299112 8.1:-42789815 MS.gene63085:CDS
TTACATATTGCTGCATCTTT+GGG 0.303914 8.1:-42790553 MS.gene63085:CDS
TGTGGATAGAGGCGAGGATA+TGG 0.307041 8.1:+42788242 None:intergenic
ACCGATTTCCCAGCATTTCC+TGG 0.316551 8.1:+42789521 None:intergenic
AACATGAACACCACAAATAA+TGG 0.319946 8.1:-42790691 MS.gene63085:CDS
TACATCTATAGTCTCCAAAA+TGG 0.341857 8.1:+42790597 None:intergenic
TGGAGTAGAAATAGTAGAAT+TGG 0.356230 8.1:+42789541 None:intergenic
AATGAGACACCATACTCTTT+TGG 0.370283 8.1:+42790428 None:intergenic
GTGAATCCTTTCCTGCAATT+GGG 0.374319 8.1:+42788125 None:intergenic
CCCTTCCTTTCTGTGGATAG+AGG 0.394022 8.1:+42788231 None:intergenic
GCAAAACGGCCAAAAGAGTA+TGG 0.396272 8.1:-42790437 MS.gene63085:CDS
CAATTATAGTCCACAGCATC+TGG 0.407883 8.1:-42788297 MS.gene63085:intron
GAAAGGAAGGGTGATCCATC+AGG 0.412184 8.1:-42788219 MS.gene63085:CDS
GCAACTGCGATTTATCAATC+AGG 0.412282 8.1:-42789632 MS.gene63085:CDS
CAATCGGTCACCATTATTTG+TGG 0.416034 8.1:+42790681 None:intergenic
TCCAGGAAATGCTGGGAAAT+CGG 0.424397 8.1:-42789522 MS.gene63085:intron
GTTAAGTATTCAATAGATTC+CGG 0.434390 8.1:+42790289 None:intergenic
GACGAGAAGGGCTCAAATGC+TGG 0.437592 8.1:-42790176 MS.gene63085:CDS
ATTTCTACTCCAGGAAATGC+TGG 0.442221 8.1:-42789530 MS.gene63085:CDS
TGTTAAGAATGGACAGTTTG+AGG 0.445212 8.1:-42790236 MS.gene63085:CDS
TTTGAGCTGCAGCTTTCAAT+CGG 0.458772 8.1:+42790665 None:intergenic
TTGAAAGCTGCAGCTCAAAC+AGG 0.459112 8.1:-42790661 MS.gene63085:CDS
TGTTGCGTGCCTCATCCCTC+TGG 0.465483 8.1:+42790137 None:intergenic
AGTAGAAATAGTAGAATTGG+AGG 0.467867 8.1:+42789544 None:intergenic
TTTAGCTTCCATGCAAATGA+AGG 0.468422 8.1:+42790493 None:intergenic
ACAGCATCTGGTGAGACTTC+CGG 0.468819 8.1:-42788285 MS.gene63085:CDS
GGGAGAGATTCCCCAATTGC+AGG 0.472679 8.1:-42788136 MS.gene63085:CDS
AATAATATACTCTTCACATC+TGG 0.474515 8.1:+42789824 None:intergenic
TCACTTTCTCTCCTTGTGTC+CGG 0.479671 8.1:-42790308 MS.gene63085:CDS
TTCACAATTTCACTGGCAAA+CGG 0.481304 8.1:+42790523 None:intergenic
CTCTTCCTTTAATTCTAACA+AGG 0.482377 8.1:+42790386 None:intergenic
ATTTAAAACCTTCATTTGCA+TGG 0.492908 8.1:-42790501 MS.gene63085:CDS
GCCTCTATCCACAGAAAGGA+AGG 0.498956 8.1:-42788232 MS.gene63085:CDS
GTGGATAGAGGCGAGGATAT+GGG 0.501129 8.1:+42788243 None:intergenic
AGATTCCCCAATTGCAGGAA+AGG 0.505239 8.1:-42788131 MS.gene63085:CDS
CCACAATTTCACATATGCTC+AGG 0.510997 8.1:-42789760 MS.gene63085:CDS
CTGCATATTGCTGTTAAGAA+TGG 0.514090 8.1:-42790247 MS.gene63085:CDS
TGGATCACCCTTCCTTTCTG+TGG 0.516379 8.1:+42788224 None:intergenic
AATTCTACTATTTCTACTCC+AGG 0.522570 8.1:-42789539 MS.gene63085:CDS
ATCCATCTCGCTATGCAAAA+CGG 0.525024 8.1:-42790451 MS.gene63085:CDS
ACTTCCGGAGTCACTAGCAT+TGG 0.542142 8.1:-42788270 MS.gene63085:CDS
ATTTGACTTGCAAAATGAAG+AGG 0.542519 8.1:+42790352 None:intergenic
GTTAGAATTAAAGGAAGAGA+AGG 0.544056 8.1:-42790382 MS.gene63085:CDS
CCTGAGCATATGTGAAATTG+TGG 0.544491 8.1:+42789760 None:intergenic
CTAACCTGATAAATTCCACT+AGG 0.545660 8.1:+42789599 None:intergenic
AAAGATGCAGCAATATGTAA+AGG 0.545982 8.1:+42790556 None:intergenic
TGAATCCTTTCCTGCAATTG+GGG 0.546499 8.1:+42788126 None:intergenic
GATTCTGTAAATGCCCCTGA+TGG 0.550591 8.1:+42788204 None:intergenic
TCTATAGTCTCCAAAATGGA+TGG 0.559736 8.1:+42790601 None:intergenic
AAAACCAATGCTAGTGACTC+CGG 0.562766 8.1:+42788266 None:intergenic
AAAGGAAGGGTGATCCATCA+GGG 0.566200 8.1:-42788218 MS.gene63085:CDS
TCTCGTAAAGAGCATGGAGA+GGG 0.569104 8.1:-42788157 MS.gene63085:CDS
CCTCGCCTCTATCCACAGAA+AGG 0.574333 8.1:-42788236 MS.gene63085:CDS
AATATTAAACCAGAGGGATG+AGG 0.576705 8.1:-42790146 MS.gene63085:CDS
GCATCAGTGACTTCTATGCT+AGG 0.577654 8.1:+42789785 None:intergenic
TTTGCATAGCGAGATGGATG+GGG 0.579281 8.1:+42790455 None:intergenic
TTTCTACTCCAGGAAATGCT+GGG 0.579847 8.1:-42789529 MS.gene63085:CDS
TTCAATAGATTCCGGACACA+AGG 0.579993 8.1:+42790297 None:intergenic
AAATAGAATATTAAACCAGA+GGG 0.585975 8.1:-42790152 MS.gene63085:CDS
CCAACTGTTACGTTGCTCCT+CGG 0.590493 8.1:+42789676 None:intergenic
CATCTTGAATTACTGTGTAG+AGG 0.592515 8.1:+42790626 None:intergenic
TCAAAACAAACATGACGAGA+AGG 0.592799 8.1:-42790189 MS.gene63085:CDS
AAAATGTCTCGTAAAGAGCA+TGG 0.595075 8.1:-42788163 MS.gene63085:CDS
CAAAACAAACATGACGAGAA+GGG 0.597431 8.1:-42790188 MS.gene63085:CDS
TAACCTGATAAATTCCACTA+GGG 0.599293 8.1:+42789600 None:intergenic
GGAAGTCTCACCAGATGCTG+TGG 0.600149 8.1:+42788287 None:intergenic
CCTTTCTGTGGATAGAGGCG+AGG 0.602132 8.1:+42788236 None:intergenic
AGCACTACGTAGTGACCCGC+AGG 0.620046 8.1:-42790098 MS.gene63085:intron
CTGAGCATATGTGAAATTGT+GGG 0.623590 8.1:+42789761 None:intergenic
AAAATAGAATATTAAACCAG+AGG 0.630543 8.1:-42790153 MS.gene63085:CDS
CCGAGGAGCAACGTAACAGT+TGG 0.630579 8.1:-42789676 MS.gene63085:CDS
CCTCTATCCACAGAAAGGAA+GGG 0.633997 8.1:-42788231 MS.gene63085:CDS
GCATGGAAGCTAAATCCGCA+AGG 0.644146 8.1:-42790484 MS.gene63085:CDS
AACCTGATAAATTCCACTAG+GGG 0.652837 8.1:+42789601 None:intergenic
GCTGGGTCAGAAGAGAGAAG+AGG 0.656561 8.1:+42788080 None:intergenic
GTCTCGTAAAGAGCATGGAG+AGG 0.662408 8.1:-42788158 MS.gene63085:CDS
CTCGTAAAGAGCATGGAGAG+GGG 0.680630 8.1:-42788156 MS.gene63085:CDS
AGTGCTTGCTATGTCTAATG+CGG 0.681177 8.1:+42789847 None:intergenic
CTATTGAATACTTAACTGTG+AGG 0.688811 8.1:-42790282 MS.gene63085:CDS
ACCTGATAAATTCCACTAGG+GGG 0.696093 8.1:+42789602 None:intergenic
AAGGAAGGGTGATCCATCAG+GGG 0.700546 8.1:-42788217 MS.gene63085:CDS
TGGATGGGGCTAAATCCTTG+CGG 0.703644 8.1:+42790469 None:intergenic
CATTAGAAGAAATATAACCG+AGG 0.729163 8.1:-42789693 MS.gene63085:CDS
GTTAGTGCTAGTGATGACAA+TGG 0.762091 8.1:-42789581 MS.gene63085:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAAACAAAAAATAAAATT+TGG - chr8.1:42790089-42790108 MS.gene63085:intron 10.0%
!! AAATAGGATGAATAAAATAA+TGG + chr8.1:42789464-42789483 None:intergenic 15.0%
!!! AAGAGTATATTATTAAGTTT+TGG - chr8.1:42788959-42788978 MS.gene63085:intron 15.0%
!!! ATATTTTTTATGATACCATA+TGG - chr8.1:42789346-42789365 MS.gene63085:intron 15.0%
!!! TCATATTTCTTATTACTTTT+TGG - chr8.1:42788741-42788760 MS.gene63085:intron 15.0%
!!! TTTTCTTGTTTTTCTATTTT+GGG + chr8.1:42789950-42789969 None:intergenic 15.0%
!!! TTTTTTGTTAGAAATAAGAT+AGG - chr8.1:42788784-42788803 MS.gene63085:intron 15.0%
!! AAAATAGAATATTAAACCAG+AGG - chr8.1:42788621-42788640 MS.gene63085:intron 20.0%
!! AAATAGAAAAACAAGAAAAC+TGG - chr8.1:42789951-42789970 MS.gene63085:intron 20.0%
!! AAATAGAATATTAAACCAGA+GGG - chr8.1:42788622-42788641 MS.gene63085:intron 20.0%
!! AAATTTATGTTCAAAGAATC+TGG - chr8.1:42788895-42788914 MS.gene63085:intron 20.0%
!! AGTATGTAACAATAATAGAA+GGG + chr8.1:42788867-42788886 None:intergenic 20.0%
!!! GTTTTCTTGTTTTTCTATTT+TGG + chr8.1:42789951-42789970 None:intergenic 20.0%
!!! TATGTATGATATTCTGTTTT+GGG + chr8.1:42790297-42790316 None:intergenic 20.0%
!!! TTAGCTATTTATTTTCTATG+TGG - chr8.1:42789413-42789432 MS.gene63085:intron 20.0%
!!! TTATGTATGATATTCTGTTT+TGG + chr8.1:42790298-42790317 None:intergenic 20.0%
!!! TTTTAAATTCACAATTTCAC+TGG + chr8.1:42788261-42788280 None:intergenic 20.0%
! AAAATTACAAATCGACGTTA+AGG + chr8.1:42789701-42789720 None:intergenic 25.0%
! AAAGACCTTGTTAGAATTAA+AGG - chr8.1:42788383-42788402 MS.gene63085:intron 25.0%
! AATAATATACTCTTCACATC+TGG + chr8.1:42788953-42788972 None:intergenic 25.0%
! ACAGAATATCATACATAAGA+AGG - chr8.1:42790300-42790319 MS.gene63085:CDS 25.0%
! AGAAACATCAATAAAGCTTA+TGG - chr8.1:42789596-42789615 MS.gene63085:CDS 25.0%
! ATGATACCATATGGAAAAAT+AGG - chr8.1:42789355-42789374 MS.gene63085:intron 25.0%
! ATTGATTATTCGATGTTTAG+AGG - chr8.1:42789894-42789913 MS.gene63085:intron 25.0%
! CAGTATGTAACAATAATAGA+AGG + chr8.1:42788868-42788887 None:intergenic 25.0%
! CGCTAAAATTGATACAAAAA+AGG + chr8.1:42789326-42789345 None:intergenic 25.0%
! CTAACAGAAATGAACTAAAT+AGG + chr8.1:42789480-42789499 None:intergenic 25.0%
! CTTCTATTATTGTTACATAC+TGG - chr8.1:42788866-42788885 MS.gene63085:intron 25.0%
! GGTAAAAATAGTAGAATTGA+AGG + chr8.1:42790238-42790257 None:intergenic 25.0%
! GTTAAGTATTCAATAGATTC+CGG + chr8.1:42788488-42788507 None:intergenic 25.0%
! TGATACCATATGGAAAAATA+GGG - chr8.1:42789356-42789375 MS.gene63085:intron 25.0%
!! ATTTAAAACCTTCATTTGCA+TGG - chr8.1:42788273-42788292 MS.gene63085:CDS 25.0%
!! GATATTATTGGTTGTGAATT+TGG + chr8.1:42789676-42789695 None:intergenic 25.0%
!! TACAACATAGAATTTGTTTC+TGG + chr8.1:42790045-42790064 None:intergenic 25.0%
!!! TCACAATTTGCTAAGATTTA+CGG + chr8.1:42789747-42789766 None:intergenic 25.0%
AAAGACCATCACACAAATTA+AGG + chr8.1:42789865-42789884 None:intergenic 30.0%
AAAGATGCAGCAATATGTAA+AGG + chr8.1:42788221-42788240 None:intergenic 30.0%
AGTAGAAATAGTAGAATTGG+AGG + chr8.1:42789233-42789252 None:intergenic 30.0%
AGTCAAAGCTTATTCATACA+TGG + chr8.1:42790160-42790179 None:intergenic 30.0%
ATATTCTCTTATAGATCCCA+GGG - chr8.1:42790345-42790364 MS.gene63085:CDS 30.0%
ATTTGGATCAACTCAAGATT+TGG - chr8.1:42790106-42790125 MS.gene63085:CDS 30.0%
CATTAGAAGAAATATAACCG+AGG - chr8.1:42789081-42789100 MS.gene63085:intron 30.0%
CTATTGAATACTTAACTGTG+AGG - chr8.1:42788492-42788511 MS.gene63085:intron 30.0%
CTCTTCCTTTAATTCTAACA+AGG + chr8.1:42788391-42788410 None:intergenic 30.0%
GTTAGAATTAAAGGAAGAGA+AGG - chr8.1:42788392-42788411 MS.gene63085:intron 30.0%
TAACCTGATAAATTCCACTA+GGG + chr8.1:42789177-42789196 None:intergenic 30.0%
TAATGGAATTGACATGTGTA+GGG + chr8.1:42789447-42789466 None:intergenic 30.0%
TACATCTATAGTCTCCAAAA+TGG + chr8.1:42788180-42788199 None:intergenic 30.0%
TGGAGTAGAAATAGTAGAAT+TGG + chr8.1:42789236-42789255 None:intergenic 30.0%
TTACATATTGCTGCATCTTT+GGG - chr8.1:42788221-42788240 MS.gene63085:CDS 30.0%
TTACTGCATAAGTGAGTTAT+TGG + chr8.1:42789982-42790001 None:intergenic 30.0%
TTTACATATTGCTGCATCTT+TGG - chr8.1:42788220-42788239 MS.gene63085:CDS 30.0%
! ATAATGGAATTGACATGTGT+AGG + chr8.1:42789448-42789467 None:intergenic 30.0%
! CTTCATTTTGCAAGTCAAAT+TGG - chr8.1:42788425-42788444 MS.gene63085:intron 30.0%
!! AATTCTACTATTTCTACTCC+AGG - chr8.1:42789235-42789254 MS.gene63085:intron 30.0%
!! AGAAACCCTATTTTTCCATA+TGG + chr8.1:42789364-42789383 None:intergenic 30.0%
!! ATTTGACTTGCAAAATGAAG+AGG + chr8.1:42788425-42788444 None:intergenic 30.0%
!! CTCTTCAATTACTTGTTATC+TGG - chr8.1:42788561-42788580 MS.gene63085:intron 30.0%
!! GCTATTTATTTTCTATGTGG+AGG - chr8.1:42789416-42789435 MS.gene63085:intron 30.0%
!! TAATTTGTGTGATGGTCTTT+TGG - chr8.1:42789865-42789884 MS.gene63085:CDS 30.0%
AAAAATGACGTCAATCACCA+TGG + chr8.1:42789503-42789522 None:intergenic 35.0%
AAAATGTCTCGTAAAGAGCA+TGG - chr8.1:42790611-42790630 MS.gene63085:CDS 35.0%
AACCTGATAAATTCCACTAG+GGG + chr8.1:42789176-42789195 None:intergenic 35.0%
AATATTAAACCAGAGGGATG+AGG - chr8.1:42788628-42788647 MS.gene63085:intron 35.0%
AATGGAATTGACATGTGTAG+GGG + chr8.1:42789446-42789465 None:intergenic 35.0%
AGAATATGCATCTCTTACCA+AGG + chr8.1:42790332-42790351 None:intergenic 35.0%
AGAGTTTATCAGATTAGGGT+CGG - chr8.1:42790189-42790208 MS.gene63085:CDS 35.0%
CAAAACAAACATGACGAGAA+GGG - chr8.1:42788586-42788605 MS.gene63085:intron 35.0%
CATATTCTCTTATAGATCCC+AGG - chr8.1:42790344-42790363 MS.gene63085:CDS 35.0%
CATCTTGAATTACTGTGTAG+AGG + chr8.1:42788151-42788170 None:intergenic 35.0%
CTAACCTGATAAATTCCACT+AGG + chr8.1:42789178-42789197 None:intergenic 35.0%
CTGAGCATATGTGAAATTGT+GGG + chr8.1:42789016-42789035 None:intergenic 35.0%
GAATATGCATCTCTTACCAA+GGG + chr8.1:42790331-42790350 None:intergenic 35.0%
GACGTTAAGGTCGATATTAT+TGG + chr8.1:42789688-42789707 None:intergenic 35.0%
GCTTATGGTTGTATAGATGA+TGG - chr8.1:42789611-42789630 MS.gene63085:CDS 35.0%
TAATCTGATAAACTCTCCCA+GGG + chr8.1:42790185-42790204 None:intergenic 35.0%
TAGTTCATTTCTGTTAGCCA+TGG - chr8.1:42789483-42789502 MS.gene63085:intron 35.0%
TCAAAACAAACATGACGAGA+AGG - chr8.1:42788585-42788604 MS.gene63085:intron 35.0%
TCTATAGTCTCCAAAATGGA+TGG + chr8.1:42788176-42788195 None:intergenic 35.0%
TGGGAGAGTTTATCAGATTA+GGG - chr8.1:42790185-42790204 MS.gene63085:CDS 35.0%
TGTTAAGAATGGACAGTTTG+AGG - chr8.1:42788538-42788557 MS.gene63085:intron 35.0%
TTAGAGGACTAACTGTTGTT+TGG - chr8.1:42789910-42789929 MS.gene63085:intron 35.0%
TTCACAATTTCACTGGCAAA+CGG + chr8.1:42788254-42788273 None:intergenic 35.0%
TTCTATGTTGTAGCAGATTC+CGG - chr8.1:42790053-42790072 MS.gene63085:intron 35.0%
! AATGAGACACCATACTCTTT+TGG + chr8.1:42788349-42788368 None:intergenic 35.0%
! AGATGCTGTGGACTATAATT+GGG + chr8.1:42790478-42790497 None:intergenic 35.0%
! CTGCATATTGCTGTTAAGAA+TGG - chr8.1:42788527-42788546 MS.gene63085:intron 35.0%
! TCAAGATGATCCATCCATTT+TGG - chr8.1:42788163-42788182 MS.gene63085:CDS 35.0%
! TGGAAAAATAGGGTTTCTAG+TGG - chr8.1:42789366-42789385 MS.gene63085:intron 35.0%
! TTTAGCTTCCATGCAAATGA+AGG + chr8.1:42788284-42788303 None:intergenic 35.0%
! TTTTGCAAGTCAAATTGGTG+AGG - chr8.1:42788430-42788449 MS.gene63085:intron 35.0%
!! ATGGTGATTGACGTCATTTT+TGG - chr8.1:42789502-42789521 MS.gene63085:intron 35.0%
!! CAAGTCCTTAATTTGTGTGA+TGG - chr8.1:42789857-42789876 MS.gene63085:CDS 35.0%
!!! TCATTTTTGGAGTGTACACA+AGG - chr8.1:42789515-42789534 MS.gene63085:intron 35.0%
!!! TGGTTTTGTTGTTATTGCCT+TGG + chr8.1:42790393-42790412 None:intergenic 35.0%
!!! TTTTGTTGTTATTGCCTTGG+AGG + chr8.1:42790390-42790409 None:intergenic 35.0%
AAAACCAATGCTAGTGACTC+CGG + chr8.1:42790511-42790530 None:intergenic 40.0%
AAGTAGAACACCATTACCTG+CGG + chr8.1:42788695-42788714 None:intergenic 40.0%
AATCTGATAAACTCTCCCAG+GGG + chr8.1:42790184-42790203 None:intergenic 40.0%
ACAGGAATGCAAAAGATCTC+AGG + chr8.1:42790422-42790441 None:intergenic 40.0%
ACCTGATAAATTCCACTAGG+GGG + chr8.1:42789175-42789194 None:intergenic 40.0%
ATCCATCTCGCTATGCAAAA+CGG - chr8.1:42788323-42788342 MS.gene63085:intron 40.0%
ATTTCTACTCCAGGAAATGC+TGG - chr8.1:42789244-42789263 MS.gene63085:intron 40.0%
CAATCGGTCACCATTATTTG+TGG + chr8.1:42788096-42788115 None:intergenic 40.0%
CAATTATAGTCCACAGCATC+TGG - chr8.1:42790477-42790496 MS.gene63085:CDS 40.0%
CCACAATTTCACATATGCTC+AGG - chr8.1:42789014-42789033 MS.gene63085:intron 40.0%
CCTGAGCATATGTGAAATTG+TGG + chr8.1:42789017-42789036 None:intergenic 40.0%
CTAATCTGATAAACTCTCCC+AGG + chr8.1:42790186-42790205 None:intergenic 40.0%
CTGGGAGAGTTTATCAGATT+AGG - chr8.1:42790184-42790203 MS.gene63085:CDS 40.0%
CTTCAATTTCTTCGTAGCAC+AGG - chr8.1:42788841-42788860 MS.gene63085:intron 40.0%
GCAACTGCGATTTATCAATC+AGG - chr8.1:42789142-42789161 MS.gene63085:intron 40.0%
GGCAATAACAACAAAACCAG+TGG - chr8.1:42790394-42790413 MS.gene63085:CDS 40.0%
GTGAATCCTTTCCTGCAATT+GGG + chr8.1:42790652-42790671 None:intergenic 40.0%
GTTAGTGCTAGTGATGACAA+TGG - chr8.1:42789193-42789212 MS.gene63085:intron 40.0%
TGAATCCTTTCCTGCAATTG+GGG + chr8.1:42790651-42790670 None:intergenic 40.0%
TTAGGGTCGGTGACAATAAT+TGG - chr8.1:42790202-42790221 MS.gene63085:CDS 40.0%
TTCAATAGATTCCGGACACA+AGG + chr8.1:42788480-42788499 None:intergenic 40.0%
TTTCTACTCCAGGAAATGCT+GGG - chr8.1:42789245-42789264 MS.gene63085:intron 40.0%
TTTGAGCTGCAGCTTTCAAT+CGG + chr8.1:42788112-42788131 None:intergenic 40.0%
! AGATGATGGTACGATGACTA+CGG - chr8.1:42789625-42789644 MS.gene63085:CDS 40.0%
! CAGATGCTGTGGACTATAAT+TGG + chr8.1:42790479-42790498 None:intergenic 40.0%
! TTTCTAGTGGCTTCTCCAAT+GGG - chr8.1:42789379-42789398 MS.gene63085:intron 40.0%
! TTTTGCATAGCGAGATGGAT+GGG + chr8.1:42788323-42788342 None:intergenic 40.0%
!! AGTGCTTGCTATGTCTAATG+CGG + chr8.1:42788930-42788949 None:intergenic 40.0%
!! CTTTACGAGACATTTTGACG+AGG + chr8.1:42790607-42790626 None:intergenic 40.0%
!!! CTATTTTTACCCCTCGAATG+TGG - chr8.1:42790247-42790266 MS.gene63085:CDS 40.0%
AAAGGAAGGGTGATCCATCA+GGG - chr8.1:42790556-42790575 MS.gene63085:CDS 45.0%
ACTGCAACTGAAACATCTGC+TGG - chr8.1:42789650-42789669 MS.gene63085:CDS 45.0%
AGATTCCCCAATTGCAGGAA+AGG - chr8.1:42790643-42790662 MS.gene63085:CDS 45.0%
AGTAGAACACCATTACCTGC+GGG + chr8.1:42788694-42788713 None:intergenic 45.0%
ATCTGATAAACTCTCCCAGG+GGG + chr8.1:42790183-42790202 None:intergenic 45.0%
ATGATGGTACGATGACTACG+GGG - chr8.1:42789627-42789646 MS.gene63085:CDS 45.0%
CAAAAGATCTCAGGTACCAC+TGG + chr8.1:42790413-42790432 None:intergenic 45.0%
CCTCTATCCACAGAAAGGAA+GGG - chr8.1:42790543-42790562 MS.gene63085:CDS 45.0%
CGTGAATCCTTTCCTGCAAT+TGG + chr8.1:42790653-42790672 None:intergenic 45.0%
CTAACAGTAAGCCATCCCAT+TGG + chr8.1:42789397-42789416 None:intergenic 45.0%
GATTCTGTAAATGCCCCTGA+TGG + chr8.1:42790573-42790592 None:intergenic 45.0%
GCAAAACGGCCAAAAGAGTA+TGG - chr8.1:42788337-42788356 MS.gene63085:intron 45.0%
GCATCAGTGACTTCTATGCT+AGG + chr8.1:42788992-42789011 None:intergenic 45.0%
TACCCCTCGAATGTGGTAAA+GGG - chr8.1:42790254-42790273 MS.gene63085:CDS 45.0%
TCACTTTCTCTCCTTGTGTC+CGG - chr8.1:42788466-42788485 MS.gene63085:intron 45.0%
TCCAGGAAATGCTGGGAAAT+CGG - chr8.1:42789252-42789271 MS.gene63085:intron 45.0%
TCCCCCTAGTGGAATTTATC+AGG - chr8.1:42789171-42789190 MS.gene63085:intron 45.0%
TGGACTCACGTTCTTCTTAC+AGG + chr8.1:42790440-42790459 None:intergenic 45.0%
TTACCCCTCGAATGTGGTAA+AGG - chr8.1:42790253-42790272 MS.gene63085:CDS 45.0%
TTATTGCCTTGGAGGACAAG+TGG + chr8.1:42790382-42790401 None:intergenic 45.0%
TTGAAAGCTGCAGCTCAAAC+AGG - chr8.1:42788113-42788132 MS.gene63085:CDS 45.0%
TTTGCATAGCGAGATGGATG+GGG + chr8.1:42788322-42788341 None:intergenic 45.0%
! AATAAGCTTTGACTCCCCCT+GGG - chr8.1:42790166-42790185 MS.gene63085:CDS 45.0%
! GAGGACTAACTGTTGTTTGG+AGG - chr8.1:42789913-42789932 MS.gene63085:intron 45.0%
! GATGATGGTACGATGACTAC+GGG - chr8.1:42789626-42789645 MS.gene63085:CDS 45.0%
! GTTTTGCATAGCGAGATGGA+TGG + chr8.1:42788324-42788343 None:intergenic 45.0%
! TCTCGTAAAGAGCATGGAGA+GGG - chr8.1:42790617-42790636 MS.gene63085:CDS 45.0%
!! GCTGGGAAATCGGTTTTATC+AGG - chr8.1:42789262-42789281 MS.gene63085:intron 45.0%
!! GTTTCTAGTGGCTTCTCCAA+TGG - chr8.1:42789378-42789397 MS.gene63085:intron 45.0%
AAGGAAGGGTGATCCATCAG+GGG - chr8.1:42790557-42790576 MS.gene63085:CDS 50.0%
ACAGCATCTGGTGAGACTTC+CGG - chr8.1:42790489-42790508 MS.gene63085:CDS 50.0%
ACATAAGAAGGCAACGCCCT+TGG - chr8.1:42790312-42790331 MS.gene63085:CDS 50.0%
ACCCCTCGAATGTGGTAAAG+GGG - chr8.1:42790255-42790274 MS.gene63085:CDS 50.0%
ACTCCCCTTTACCACATTCG+AGG + chr8.1:42790261-42790280 None:intergenic 50.0%
ACTTCCGGAGTCACTAGCAT+TGG - chr8.1:42790504-42790523 MS.gene63085:CDS 50.0%
CCAACTGTTACGTTGCTCCT+CGG + chr8.1:42789101-42789120 None:intergenic 50.0%
CCCTTCCTTTCTGTGGATAG+AGG + chr8.1:42790546-42790565 None:intergenic 50.0%
CTCCCCTTTACCACATTCGA+GGG + chr8.1:42790260-42790279 None:intergenic 50.0%
GAAAGGAAGGGTGATCCATC+AGG - chr8.1:42790555-42790574 MS.gene63085:CDS 50.0%
GCATGGAAGCTAAATCCGCA+AGG - chr8.1:42788290-42788309 MS.gene63085:CDS 50.0%
GCCTCTATCCACAGAAAGGA+AGG - chr8.1:42790542-42790561 MS.gene63085:CDS 50.0%
GTGGATAGAGGCGAGGATAT+GGG + chr8.1:42790534-42790553 None:intergenic 50.0%
TAGTGGCTTCTCCAATGGGA+TGG - chr8.1:42789383-42789402 MS.gene63085:intron 50.0%
TCCCCTTTACCACATTCGAG+GGG + chr8.1:42790259-42790278 None:intergenic 50.0%
TGGATCACCCTTCCTTTCTG+TGG + chr8.1:42790553-42790572 None:intergenic 50.0%
TGGATGGGGCTAAATCCTTG+CGG + chr8.1:42788308-42788327 None:intergenic 50.0%
TGTGGATAGAGGCGAGGATA+TGG + chr8.1:42790535-42790554 None:intergenic 50.0%
TTGGGTATGTCCAGACGTTC+TGG + chr8.1:42790460-42790479 None:intergenic 50.0%
TTTCTTCCACGCTGCATAGC+CGG + chr8.1:42790075-42790094 None:intergenic 50.0%
! AACTTGCCACTTGTCCTCCA+AGG - chr8.1:42790373-42790392 MS.gene63085:CDS 50.0%
! ACCGATTTCCCAGCATTTCC+TGG + chr8.1:42789256-42789275 None:intergenic 50.0%
! CTCGTAAAGAGCATGGAGAG+GGG - chr8.1:42790618-42790637 MS.gene63085:CDS 50.0%
! GAATAAGCTTTGACTCCCCC+TGG - chr8.1:42790165-42790184 MS.gene63085:CDS 50.0%
! GTCTCGTAAAGAGCATGGAG+AGG - chr8.1:42790616-42790635 MS.gene63085:CDS 50.0%
ACGTAGTGACCCGCAGGTAA+TGG - chr8.1:42788682-42788701 MS.gene63085:intron 55.0%
CCGAGGAGCAACGTAACAGT+TGG - chr8.1:42789098-42789117 MS.gene63085:intron 55.0%
CCTCGCCTCTATCCACAGAA+AGG - chr8.1:42790538-42790557 MS.gene63085:CDS 55.0%
CCTTTCTGTGGATAGAGGCG+AGG + chr8.1:42790541-42790560 None:intergenic 55.0%
GAACGTGAGTCCAGAACGTC+TGG - chr8.1:42790447-42790466 MS.gene63085:CDS 55.0%
GACGAGAAGGGCTCAAATGC+TGG - chr8.1:42788598-42788617 MS.gene63085:intron 55.0%
GGAAGTCTCACCAGATGCTG+TGG + chr8.1:42790490-42790509 None:intergenic 55.0%
GGGAGAGATTCCCCAATTGC+AGG - chr8.1:42790638-42790657 MS.gene63085:CDS 55.0%
TCAGGATTGAGTCCCCCTAG+TGG - chr8.1:42789160-42789179 MS.gene63085:intron 55.0%
! AAGTGGCAAGTTGAGTGCCC+TGG + chr8.1:42790365-42790384 None:intergenic 55.0%
! AGTGGCAAGTTGAGTGCCCT+GGG + chr8.1:42790364-42790383 None:intergenic 55.0%
!! GGCCGTTTTGCATAGCGAGA+TGG + chr8.1:42788328-42788347 None:intergenic 55.0%
AGCACTACGTAGTGACCCGC+AGG - chr8.1:42788676-42788695 MS.gene63085:intron 60.0%
CAGATTCCGGCTATGCAGCG+TGG - chr8.1:42790066-42790085 MS.gene63085:intron 60.0%
TGTTGCGTGCCTCATCCCTC+TGG + chr8.1:42788640-42788659 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 42788086 42790710 42788086 ID=MS.gene63085
chr8.1 mRNA 42788086 42790710 42788086 ID=MS.gene63085.t1;Parent=MS.gene63085
chr8.1 exon 42790099 42790710 42790099 ID=MS.gene63085.t1.exon1;Parent=MS.gene63085.t1
chr8.1 CDS 42790099 42790710 42790099 ID=cds.MS.gene63085.t1;Parent=MS.gene63085.t1
chr8.1 exon 42789523 42789885 42789523 ID=MS.gene63085.t1.exon2;Parent=MS.gene63085.t1
chr8.1 CDS 42789523 42789885 42789523 ID=cds.MS.gene63085.t1;Parent=MS.gene63085.t1
chr8.1 exon 42788086 42788310 42788086 ID=MS.gene63085.t1.exon3;Parent=MS.gene63085.t1
chr8.1 CDS 42788086 42788310 42788086 ID=cds.MS.gene63085.t1;Parent=MS.gene63085.t1
Gene Sequence

>MS.gene63085

ATGAACACCACAAATAATGGTGACCGATTGAAAGCTGCAGCTCAAACAGGTGATATAGATCTCCTCTACACAGTAATTCAAGATGATCCATCCATTTTGGAGACTATAGATGTAATGTCATTTGTTGAAACTCCTTTACATATTGCTGCATCTTTGGGTCATATGCCGTTTGCCAGTGAAATTGTGAATTTAAAACCTTCATTTGCATGGAAGCTAAATCCGCAAGGATTTAGCCCCATCCATCTCGCTATGCAAAACGGCCAAAAGAGTATGGTGTCTCATTTTGTGCACAACAATAAAGACCTTGTTAGAATTAAAGGAAGAGAAGGTATAACTCCTCTTCATTTTGCAAGTCAAATTGGTGAGGTTAATCATTTAGCTCACTTTCTCTCCTTGTGTCCGGAATCTATTGAATACTTAACTGTGAGGCACGAGACTGCACTGCATATTGCTGTTAAGAATGGACAGTTTGAGGCTCTTCAATTACTTGTTATCTGGCTCAAAACAAACATGACGAGAAGGGCTCAAATGCTGGAAAATAGAATATTAAACCAGAGGGATGAGGCACGCAACACGATTTTGCATATTTCAGCACTACGTAGTGACCCGCAGGTAATGGTGTTCTACTTATAAGTCATCTACTTCTAAATGATGATCATATTTCTTATTACTTTTTGGTGTTCTACTTCTAAGTCGTCTTTTTTGTTAGAAATAAGATAGGTTTAAAATGAATGATCTCTAATAATTGTATTGTTCTTCAATTTCTTCGTAGCACAGGCCCTTCTATTATTGTTACATACTGGTCGTATAAATTTATGTTCAAAGAATCTGGAGAACAAAACCGCATTAGACATAGCAAGCACTCCAGATGTGAAGAGTATATTATTAAGTTTTGGAGCAAAACCTAGCATAGAAGTCACTGATGCTCCCACAATTTCACATATGCTCAGGTTAAAGATCACAATTAGAGAGAATCTGTTCATTTCTATAAATCGCATTAGAAGAAATATAACCGAGGAGCAACGTAACAGTTGGTTGATAGTTGCGACTCTTGTTGCAACTGCGATTTATCAATCAGGATTGAGTCCCCCTAGTGGAATTTATCAGGTTAGTGCTAGTGATGACAATGGTGTGAACATCACTTCCTCCAATTCTACTATTTCTACTCCAGGAAATGCTGGGAAATCGGTTTTATCAGGATATGAATTCTTTTTGTTTTTATTTATAAACATGTATTCCTTTTTTGTATCAATTTTAGCGATATTTTTTATGATACCATATGGAAAAATAGGGTTTCTAGTGGCTTCTCCAATGGGATGGCTTACTGTTAGCTATTTATTTTCTATGTGGAGGATATCCCCTACACATGTCAATTCCATTATTTTATTCATCCTATTTAGTTCATTTCTGTTAGCCATGGTGATTGACGTCATTTTTGGAGTGTACACAAGGCTTAAACACAGAATTGCTAAGATATTTAATATGATAGTTAAAGCTTTGTTGAAATAAGAGAAACATCAATAAAGCTTATGGTTGTATAGATGATGGTACGATGACTACGGGGACTGCAACTGAAACATCTGCTGGCCAAATTCACAACCAATAATATCGACCTTAACGTCGATTTGTAATTTTCATTGTCATTGCTATCTTTGTTTCCGTAAATCTTAGCAAATTGTGATTCAATGTATTAAATATTTAAATTATATCTTGTATTTTATTGAATGCTTGTGTATTCTATTAAATGTTGTTTGAAAGAAAATTTATTACGCAAGTCCTTAATTTGTGTGATGGTCTTTTGGAAGAAAATTGATTATTCGATGTTTAGAGGACTAACTGTTGTTTGGAGGAAAATTGATTACCCAAAATAGAAAAACAAGAAAACTGGAAAAACCAATAACTCACTTATGCAGTAAGTCAACTTAAATGCACTCAGTAGCAAAATGCAGTTACAATCCAGAAACAAATTCTATGTTGTAGCAGATTCCGGCTATGCAGCGTGGAAGAAACAAAAAATAAAATTTGGATCAACTCAAGATTTGGTTAATAGTCGCGTCTCTTATTGCATCTACCATGTATGAATAAGCTTTGACTCCCCCTGGGAGAGTTTATCAGATTAGGGTCGGTGACAATAATTGGAACATCACTTCCTTCAATTCTACTATTTTTACCCCTCGAATGTGGTAAAGGGGAGTTCAACGTGCAGCTCCCAAAACAGAATATCATACATAAGAAGGCAACGCCCTTGGTAAGAGATGCATATTCTCTTATAGATCCCAGGGCACTCAACTTGCCACTTGTCCTCCAAGGCAATAACAACAAAACCAGTGGTACCTGAGATCTTTTGCATTCCTGTAAGAAGAACGTGAGTCCAGAACGTCTGGACATACCCAATTATAGTCCACAGCATCTGGTGAGACTTCCGGAGTCACTAGCATTGGTTTTCCCATATCCTCGCCTCTATCCACAGAAAGGAAGGGTGATCCATCAGGGGCATTTACAGAATCTATTGTCTCAACCTCGTCAAAATGTCTCGTAAAGAGCATGGAGAGGGGAGAGATTCCCCAATTGCAGGAAAGGATTCACGTCATATTCAATAAAACTGAAGCCTCTTCTCTCTTCTGA
Protein sequence

>MS.gene63085.t1

MNTTNNGDRLKAAAQTGDIDLLYTVIQDDPSILETIDVMSFVETPLHIAASLGHMPFASEIVNLKPSFAWKLNPQGFSPIHLAMQNGQKSMVSHFVHNNKDLVRIKGREGITPLHFASQIGEVNHLAHFLSLCPESIEYLTVRHETALHIAVKNGQFEALQLLVIWLKTNMTRRAQMLENRILNQRDEARNTILHISALRSDPQNLENKTALDIASTPDVKSILLSFGAKPSIEVTDAPTISHMLRLKITIRENLFISINRIRRNITEEQRNSWLIVATLVATAIYQSGLSPPSGIYQVSASDDNGVNITSSNSTISTPGNAGKSSTASGETSGVTSIGFPISSPLSTERKGDPSGAFTESIVSTSSKCLVKSMERGEIPQLQERIHVIFNKTEASSLF