AlfalfaGEDB | MS.gene027441

M. sativa cultivar XinJiangDaYe / MS.gene027441

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF12796.10 PF13857.9 PF13962.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene027441.t1	AES77856.1	60.6	340	128	1	1	340	1	334	3.40E-95	358.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene027441.t1	Q8GYH5	37.4	305	182	3	5	302	1	303	4.3e-14	80.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene027441.t1	G7KSX2	60.6	340	128	1	1	340	1	334	2.5e-95	358.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene027441	MS.gene043742	PPI
MS.gene027441	MS.gene050457	PPI
MS.gene36842	MS.gene027441	PPI
MS.gene027441	MS.gene99973	PPI
MS.gene99554	MS.gene027441	PPI
MS.gene027441	MS.gene30174	PPI
MS.gene027441	MS.gene26021	PPI
MS.gene058128	MS.gene027441	PPI
MS.gene99588	MS.gene027441	PPI
MS.gene027075	MS.gene027441	PPI
MS.gene41291	MS.gene027441	PPI
MS.gene066501	MS.gene027441	PPI
MS.gene63085	MS.gene027441	PPI
MS.gene056455	MS.gene027441	PPI
MS.gene027441	MS.gene91775	PPI
MS.gene052956	MS.gene027441	PPI
MS.gene043662	MS.gene027441	PPI
MS.gene027441	MS.gene050270	PPI
MS.gene039682	MS.gene027441	PPI
MS.gene26532	MS.gene027441	PPI
MS.gene042783	MS.gene027441	PPI
MS.gene027441	MS.gene065848	PPI
MS.gene027441	MS.gene037184	PPI
MS.gene21734	MS.gene027441	PPI
MS.gene33377	MS.gene027441	PPI
MS.gene027441	MS.gene54764	PPI
MS.gene027441	MS.gene038519	PPI
MS.gene71204	MS.gene027441	PPI
MS.gene30172	MS.gene027441	PPI
MS.gene007486	MS.gene027441	PPI
MS.gene045937	MS.gene027441	PPI
MS.gene041770	MS.gene027441	PPI
MS.gene23852	MS.gene027441	PPI
MS.gene97943	MS.gene027441	PPI
MS.gene26672	MS.gene027441	PPI
MS.gene97379	MS.gene027441	PPI
MS.gene67157	MS.gene027441	PPI
MS.gene027441	MS.gene050272	PPI
MS.gene012203	MS.gene027441	PPI
MS.gene027441	MS.gene050456	PPI
MS.gene022109	MS.gene027441	PPI
MS.gene34651	MS.gene027441	PPI
MS.gene25244	MS.gene027441	PPI
MS.gene027441	MS.gene51258	PPI
MS.gene027441	MS.gene32723	PPI
MS.gene08470	MS.gene027441	PPI
MS.gene025229	MS.gene027441	PPI
MS.gene027441	MS.gene053363	PPI
MS.gene89986	MS.gene027441	PPI
MS.gene26019	MS.gene027441	PPI
MS.gene027441	MS.gene32726	PPI
MS.gene027441	MS.gene71122	PPI
MS.gene69687	MS.gene027441	PPI
MS.gene035805	MS.gene027441	PPI
MS.gene027441	MS.gene60920	PPI
MS.gene027441	MS.gene73119	PPI
MS.gene027441	MS.gene92243	PPI
MS.gene69965	MS.gene027441	PPI
MS.gene066500	MS.gene027441	PPI
MS.gene027441	MS.gene34009	PPI
MS.gene027441	MS.gene51262	PPI
MS.gene82740	MS.gene027441	PPI
MS.gene024753	MS.gene027441	PPI
MS.gene78281	MS.gene027441	PPI
MS.gene027441	MS.gene014931	PPI
MS.gene027441	MS.gene21888	PPI
MS.gene70008	MS.gene027441	PPI
MS.gene027441	MS.gene39623	PPI
MS.gene69966	MS.gene027441	PPI
MS.gene24806	MS.gene027441	PPI
MS.gene071113	MS.gene027441	PPI
MS.gene027071	MS.gene027441	PPI
MS.gene30433	MS.gene027441	PPI
MS.gene007487	MS.gene027441	PPI
MS.gene018518	MS.gene027441	PPI
MS.gene027441	MS.gene81055	PPI
MS.gene027441	MS.gene65775	PPI
MS.gene027441	MS.gene80163	PPI
MS.gene010910	MS.gene027441	PPI
MS.gene066464	MS.gene027441	PPI
MS.gene027441	MS.gene02469	PPI
MS.gene063222	MS.gene027441	PPI
MS.gene25515	MS.gene027441	PPI
MS.gene07706	MS.gene027441	PPI
MS.gene027074	MS.gene027441	PPI
MS.gene027441	MS.gene51278	PPI
MS.gene027441	MS.gene87218	PPI
MS.gene010912	MS.gene027441	PPI
MS.gene25024	MS.gene027441	PPI
MS.gene027441	MS.gene85534	PPI
MS.gene23811	MS.gene027441	PPI
MS.gene23810	MS.gene027441	PPI
MS.gene027441	MS.gene23881	PPI
MS.gene51335	MS.gene027441	PPI
MS.gene005995	MS.gene027441	PPI
MS.gene027441	MS.gene062662	PPI
MS.gene037950	MS.gene027441	PPI
MS.gene068249	MS.gene027441	PPI
MS.gene027441	MS.gene30177	PPI
MS.gene066046	MS.gene027441	PPI
MS.gene051003	MS.gene027441	PPI
MS.gene027441	MS.gene008833	PPI
MS.gene64441	MS.gene027441	PPI
MS.gene004844	MS.gene027441	PPI
MS.gene027441	MS.gene90400	PPI
MS.gene30176	MS.gene027441	PPI
MS.gene27223	MS.gene027441	PPI
MS.gene027441	MS.gene48655	PPI
MS.gene027441	MS.gene09785	PPI
MS.gene22482	MS.gene027441	PPI
MS.gene027441	MS.gene027072	PPI
MS.gene061932	MS.gene027441	PPI
MS.gene027441	MS.gene65463	PPI
MS.gene51262	MS.gene027441	PPI
MS.gene027441	MS.gene007485	PPI
MS.gene050270	MS.gene027441	PPI
MS.gene85534	MS.gene027441	PPI
MS.gene24922	MS.gene027441	PPI
MS.gene027073	MS.gene027441	PPI
MS.gene064542	MS.gene027441	PPI
MS.gene066467	MS.gene027441	PPI
MS.gene47479	MS.gene027441	PPI
MS.gene027441	MS.gene23196	PPI
MS.gene008767	MS.gene027441	PPI
MS.gene027441	MS.gene067705	PPI
MS.gene34712	MS.gene027441	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene027441.t1	MTR_8g072510	76.991	339	77	1	3	341	4	341	0.0	521
MS.gene027441.t1	MTR_7g021090	71.848	341	90	1	1	341	1	335	1.34e-172	487
MS.gene027441.t1	MTR_8g039620	70.571	350	91	3	6	344	11	359	1.39e-171	485
MS.gene027441.t1	MTR_8g062410	70.655	351	73	3	3	341	4	336	4.55e-167	474
MS.gene027441.t1	MTR_1g059930	66.860	344	111	1	1	341	1	344	5.27e-161	458
MS.gene027441.t1	MTR_3g016210	65.642	358	107	3	3	345	4	360	6.52e-159	453
MS.gene027441.t1	MTR_8g068990	64.183	349	103	5	8	351	11	342	1.71e-151	435
MS.gene027441.t1	MTR_7g451420	67.628	312	101	0	1	312	1	312	4.43e-147	418
MS.gene027441.t1	MTR_8g073260	61.919	344	122	3	1	340	1	339	1.05e-146	422
MS.gene027441.t1	MTR_8g069880	62.717	346	123	4	9	350	7	350	5.07e-141	409
MS.gene027441.t1	MTR_8g040930	65.878	296	91	4	51	340	1	292	1.28e-133	387
MS.gene027441.t1	MTR_8g041240	65.878	296	91	4	51	340	1	292	1.28e-133	387
MS.gene027441.t1	MTR_8g467780	57.637	347	137	2	5	341	7	353	1.17e-129	379
MS.gene027441.t1	MTR_8g066670	61.696	342	128	3	1	341	1067	1406	2.02e-126	396
MS.gene027441.t1	MTR_3g105650	49.359	312	150	2	5	309	1	311	5.20e-100	303
MS.gene027441.t1	MTR_8g073275	44.290	359	122	8	51	342	1	348	1.70e-80	253
MS.gene027441.t1	MTR_7g099580	27.010	311	176	9	7	312	10	274	4.39e-26	107
MS.gene027441.t1	MTR_1g069110	25.948	343	221	8	9	345	18	333	4.42e-25	105
MS.gene027441.t1	MTR_7g099570	30.198	202	127	3	1	197	1	193	1.99e-20	93.2
MS.gene027441.t1	MTR_2g101320	28.378	222	137	4	37	248	134	343	7.90e-20	91.3
MS.gene027441.t1	MTR_7g117620	26.370	292	171	6	44	308	119	393	1.19e-19	90.5
MS.gene027441.t1	MTR_1g115575	26.117	291	176	6	44	309	169	445	6.67e-18	85.5
MS.gene027441.t1	MTR_2g096190	25.000	288	180	6	45	308	105	380	2.76e-17	83.6
MS.gene027441.t1	MTR_2g101310	27.572	243	152	4	40	268	137	369	9.20e-17	82.0
MS.gene027441.t1	MTR_2g101310	27.572	243	152	4	40	268	137	369	9.33e-17	82.0
MS.gene027441.t1	MTR_4g062100	25.000	288	180	6	45	308	109	384	3.99e-16	80.1
MS.gene027441.t1	MTR_6g027840	30.102	196	125	6	44	230	246	438	9.51e-16	79.0
MS.gene027441.t1	MTR_2g033050	30.682	176	110	4	60	231	217	384	6.14e-15	75.9
MS.gene027441.t1	MTR_5g085830	27.723	202	127	5	59	247	293	488	1.94e-14	75.1
MS.gene027441.t1	MTR_5g085500	33.846	130	74	4	105	230	35	156	4.94e-14	73.6
MS.gene027441.t1	MTR_7g100390	24.653	288	182	6	44	308	87	362	5.19e-14	73.6
MS.gene027441.t1	MTR_1g032100	24.483	290	182	7	44	308	94	371	2.35e-13	71.6
MS.gene027441.t1	MTR_8g105260	23.793	290	184	6	44	308	101	378	4.75e-13	70.5
MS.gene027441.t1	MTR_7g100430	24.316	329	191	11	42	321	124	443	2.14e-12	68.6
MS.gene027441.t1	MTR_5g085810	28.507	221	130	7	60	267	320	525	5.37e-12	67.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene027441.t1	AT4G10720	41.250	320	180	2	8	319	4	323	2.97e-79	249
MS.gene027441.t1	AT4G10720	41.250	320	180	2	8	319	4	323	6.65e-79	249
MS.gene027441.t1	AT5G15500	39.710	345	189	5	9	345	6	339	1.16e-74	238
MS.gene027441.t1	AT1G14500	37.539	317	189	2	5	312	1	317	1.52e-73	235
MS.gene027441.t1	AT1G14480	38.390	323	189	2	8	320	4	326	7.69e-73	233
MS.gene027441.t1	AT5G54620	39.482	309	180	2	5	306	1	309	8.68e-71	228
MS.gene027441.t1	AT5G54610	34.314	306	192	3	5	303	1	304	1.97e-59	198
MS.gene027441.t1	AT1G14480	36.624	314	164	4	8	320	4	283	1.44e-56	189
MS.gene027441.t1	AT5G15500	34.836	244	140	5	110	345	1	233	6.10e-34	129
MS.gene027441.t1	AT5G51160	29.927	274	164	4	63	315	35	301	1.49e-30	121
MS.gene027441.t1	AT1G34050	26.923	260	168	5	65	312	174	423	1.90e-20	93.2
MS.gene027441.t1	AT1G03670	31.020	245	120	9	88	315	266	478	4.71e-19	89.0
MS.gene027441.t1	AT4G03500	28.384	229	130	6	92	309	274	479	1.44e-18	87.8
MS.gene027441.t1	AT4G03500	28.384	229	130	6	92	309	274	479	1.44e-18	87.8
MS.gene027441.t1	AT4G03500	28.384	229	130	6	92	309	297	502	1.62e-18	87.4
MS.gene027441.t1	AT4G03460	31.132	212	129	5	100	309	290	486	4.78e-18	85.9
MS.gene027441.t1	AT4G03460	31.132	212	129	5	100	309	258	454	5.95e-18	85.9
MS.gene027441.t1	AT2G01680	24.573	293	184	6	44	311	97	377	2.50e-16	80.5
MS.gene027441.t1	AT5G54700	29.075	227	121	7	95	316	17	208	3.46e-16	79.3
MS.gene027441.t1	AT1G10340	29.098	244	117	6	109	312	205	432	1.67e-15	78.2
MS.gene027441.t1	AT1G10340	29.098	244	117	6	109	312	201	428	1.87e-15	78.2
MS.gene027441.t1	AT1G05640	24.079	353	181	11	42	308	114	465	6.76e-15	76.6
MS.gene027441.t1	AT5G54710	27.049	244	141	7	95	312	264	496	2.92e-14	74.7
MS.gene027441.t1	AT5G54710	26.971	241	139	7	98	312	222	451	3.17e-14	74.3
MS.gene027441.t1	AT3G12360	24.746	295	175	7	44	309	155	431	3.52e-14	74.3
MS.gene027441.t1	AT3G12360	24.746	295	175	7	44	309	167	443	3.80e-14	73.9
MS.gene027441.t1	AT4G03450	25.121	207	140	4	108	310	290	485	4.93e-14	73.9
MS.gene027441.t1	AT3G09550	24.484	339	202	10	12	321	150	463	4.94e-14	73.9
MS.gene027441.t1	AT3G09550	24.484	339	202	10	12	321	150	463	4.94e-14	73.9
MS.gene027441.t1	AT4G03450	25.121	207	140	4	108	310	290	485	5.34e-14	73.6
MS.gene027441.t1	AT5G54700	25.763	295	148	9	24	316	20	245	5.73e-14	73.2
MS.gene027441.t1	AT3G09550	24.484	339	202	10	12	321	157	470	6.07e-14	73.6
MS.gene027441.t1	AT1G07710	24.768	323	207	8	9	308	70	379	1.05e-13	72.8
MS.gene027441.t1	AT1G07710	24.768	323	207	8	9	308	70	379	1.05e-13	72.8
MS.gene027441.t1	AT2G31820	25.080	311	183	10	31	308	208	501	5.18e-13	70.9
MS.gene027441.t1	AT4G03480	23.677	359	181	13	9	347	357	642	9.26e-13	70.1
MS.gene027441.t1	AT4G03480	23.677	359	181	13	9	347	360	645	1.02e-12	69.7
MS.gene027441.t1	AT5G50140	28.902	173	98	4	40	197	26	188	2.63e-12	68.6
MS.gene027441.t1	AT5G02620	22.680	291	186	6	45	308	95	373	1.24e-11	66.2
MS.gene027441.t1	AT5G02620	22.680	291	186	6	45	308	95	373	1.24e-11	66.2
MS.gene027441.t1	AT5G02620	22.680	291	186	6	45	308	95	373	1.24e-11	66.2
MS.gene027441.t1	AT5G60070	22.819	298	207	7	12	308	115	390	2.52e-11	65.5

Find 78 sgRNAs with CRISPR-Local

Find 97 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGACCTTGTTAGAGTTAA+AGG	0.117136	4.2:-53893608	MS.gene027441:CDS
GGTAAGTACTGGTATAAATT+TGG	0.243776	4.2:-53893185	MS.gene027441:CDS
GTTACATATTGCTGCATCTA+TGG	0.262404	4.2:-53893771	MS.gene027441:CDS
GGTGCTCAAAAGTTGGAAAA+TGG	0.312014	4.2:-53893386	MS.gene027441:CDS
CGGAGATGATCCATTCATTC+TGG	0.324894	4.2:-53893828	MS.gene027441:CDS
TTCATAATTTCAATGGTAAA+CGG	0.330193	4.2:+53893740	None:intergenic
TAGTGCCGAAATATGCAAAA+TGG	0.358761	4.2:+53893331	None:intergenic
TAGGGTAGAAATAGTAGAAT+TGG	0.390967	4.2:+53892832	None:intergenic
CCGATTTCCCAGCATTTCCT+AGG	0.394089	4.2:+53892813	None:intergenic
TCATAATTTCAATGGTAAAC+GGG	0.395809	4.2:+53893741	None:intergenic
TGTTGCCTGCTTTATCCCTC+TGG	0.417230	4.2:+53893354	None:intergenic
ATTTAAAACCTTCATTTGCT+TGG	0.418134	4.2:-53893718	MS.gene027441:CDS
GCAGCTGAAAGCTGCAGCTC+AGG	0.418481	4.2:-53893882	MS.gene027441:CDS
CCTAGGAAATGCTGGGAAAT+CGG	0.419453	4.2:-53892813	MS.gene027441:CDS
GGAATTTCTCTTTCTTTGTC+CGG	0.421807	4.2:-53893525	MS.gene027441:CDS
ATGATACCAGCTGGAATACT+AGG	0.430510	4.2:-53892710	MS.gene027441:CDS
CTTCTATTGTTGGTAAGTAC+TGG	0.432315	4.2:-53893196	MS.gene027441:CDS
GATCTCCTCTACGCAGTAAT+CGG	0.440555	4.2:-53893848	MS.gene027441:CDS
CCCTTCAAATACTTGTTAGC+TGG	0.445898	4.2:-53893430	MS.gene027441:CDS
ATTTCTACCCTAGGAAATGC+TGG	0.455059	4.2:-53892821	MS.gene027441:CDS
TCTATATGCTCCAGAATGAA+TGG	0.459267	4.2:+53893818	None:intergenic
AGCACAGGCACTTCTATTGT+TGG	0.470486	4.2:-53893206	MS.gene027441:intron
GTCAAGTATTCAAGGGAATC+CGG	0.474006	4.2:+53893506	None:intergenic
AATAATATACTCTTGGTCTC+TGG	0.474364	4.2:+53893115	None:intergenic
CTCTCTAGTAGGTTCTCCCA+TGG	0.474964	4.2:-53892687	MS.gene027441:CDS
GTTAGTGCCAGTGATGATAA+TGG	0.478964	4.2:-53892872	MS.gene027441:CDS
TCGAAGAGGTGCTCAAAAGT+TGG	0.483334	4.2:-53893393	MS.gene027441:CDS
GGCACTAAGTTGTGAGCCGC+AGG	0.488252	4.2:-53893315	MS.gene027441:intron
TGTAACGGAGTTTCAACAAA+TGG	0.492054	4.2:+53893788	None:intergenic
TTTAGCTTCCAAGCAAATGA+AGG	0.492400	4.2:+53893710	None:intergenic
TAGTAGGTTCTCCCATGGGT+TGG	0.493109	4.2:-53892682	MS.gene027441:CDS
GCCAGCTAACAAGTATTTGA+AGG	0.497511	4.2:+53893429	None:intergenic
AACACTTAATAATATACTCT+TGG	0.499676	4.2:+53893108	None:intergenic
TGCTTGGAAGCTAAATCCGC+AGG	0.508838	4.2:-53893702	MS.gene027441:CDS
TGTTAAGAATCAACAATATG+AGG	0.510561	4.2:-53893453	MS.gene027441:CDS
AATTCTACTATTTCTACCCT+AGG	0.517193	4.2:-53892830	MS.gene027441:CDS
ACCTCACAGTCAAGTATTCA+AGG	0.520683	4.2:+53893498	None:intergenic
GTTAGAGTTAAAGGAAGAGA+GGG	0.523986	4.2:-53893599	MS.gene027441:CDS
TTACATATTGCTGCATCTAT+GGG	0.532476	4.2:-53893770	MS.gene027441:CDS
GGAATACTAGGCTCTCTAGT+AGG	0.535653	4.2:-53892698	MS.gene027441:CDS
GTTCACGCCATTATCATCAC+TGG	0.537493	4.2:+53892865	None:intergenic
AAGAGTATATTATTAAGTGT+TGG	0.539970	4.2:-53893106	MS.gene027441:CDS
CAGAGGAGCAACGCAACACT+TGG	0.546199	4.2:-53892967	MS.gene027441:CDS
GCAACTGCGATCTATCAATC+CGG	0.547636	4.2:-53892923	MS.gene027441:CDS
GCACACGGGCACAAAGGCTC+AGG	0.547763	4.2:-53893051	MS.gene027441:CDS
ATATATGTTATGATACCAGC+TGG	0.548857	4.2:-53892719	MS.gene027441:CDS
GGTAGAAATAGTAGAATTGG+CGG	0.556035	4.2:+53892835	None:intergenic
CTGAAAGCTGCAGCTCAGGA+AGG	0.558773	4.2:-53893878	MS.gene027441:CDS
CTCTTCCTTTAACTCTAACA+AGG	0.564990	4.2:+53893603	None:intergenic
TGTTAGAGTTAAAGGAAGAG+AGG	0.566339	4.2:-53893600	MS.gene027441:CDS
AGAGAGCCTAGTATTCCAGC+TGG	0.569798	4.2:+53892704	None:intergenic
CGATTTCCCAGCATTTCCTA+GGG	0.572165	4.2:+53892814	None:intergenic
TCTCTAGTAGGTTCTCCCAT+GGG	0.580455	4.2:-53892686	MS.gene027441:CDS
AAATGGAATATTAAACCAGA+GGG	0.585975	4.2:-53893369	MS.gene027441:CDS
TTTCTACCCTAGGAAATGCT+GGG	0.590604	4.2:-53892820	MS.gene027441:CDS
CTAACCTGATAAATTCCACC+AGG	0.592230	4.2:+53892890	None:intergenic
AAATTTGGACGCAAAGAATG+AGG	0.593661	4.2:-53893170	MS.gene027441:CDS
CCAGCTAACAAGTATTTGAA+GGG	0.614265	4.2:+53893430	None:intergenic
CCCTTGAATACTTGACTGTG+AGG	0.615568	4.2:-53893499	MS.gene027441:CDS
TTAAACCAGAGGGATAAAGC+AGG	0.617620	4.2:-53893359	MS.gene027441:CDS
GAATCAGTGACTTCTAAGCT+CGG	0.618191	4.2:+53893076	None:intergenic
ACTAGCGGCAAACCAACCCA+TGG	0.623120	4.2:+53892670	None:intergenic
ATTCTCGCACACGGGCACAA+AGG	0.624210	4.2:-53893057	MS.gene027441:CDS
AAGTCACTGATTCTCGCACA+CGG	0.625915	4.2:-53893066	MS.gene027441:CDS
CCTCACAGTCAAGTATTCAA+GGG	0.642621	4.2:+53893499	None:intergenic
CTCAAACGAAATAATCGAAG+AGG	0.644802	4.2:-53893407	MS.gene027441:CDS
ATAGATGCAGCAATATGTAA+CGG	0.652232	4.2:+53893773	None:intergenic
GCTTGGAAGCTAAATCCGCA+GGG	0.653006	4.2:-53893701	MS.gene027441:CDS
AAAATGGAATATTAAACCAG+AGG	0.659194	4.2:-53893370	MS.gene027441:CDS
AGTCACTGATTCTCGCACAC+GGG	0.663891	4.2:-53893065	MS.gene027441:CDS
CATCTCCGATTACTGCGTAG+AGG	0.674210	4.2:+53893843	None:intergenic
CTAGCGGCAAACCAACCCAT+GGG	0.674900	4.2:+53892671	None:intergenic
AGTGCTTGCTATGTCTAATG+CGG	0.681177	4.2:+53893138	None:intergenic
AACCTGATAAATTCCACCAG+GGG	0.693447	4.2:+53892892	None:intergenic
TAACCTGATAAATTCCACCA+GGG	0.695982	4.2:+53892891	None:intergenic
ACCTGATAAATTCCACCAGG+GGG	0.706697	4.2:+53892893	None:intergenic
CATTAGAAGTAATATAACAG+AGG	0.709657	4.2:-53892984	MS.gene027441:CDS
TGAATGGGACTGAATCCCTG+CGG	0.749640	4.2:+53893686	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTATTTGTTAATTAATTTCT+TGG	-	chr4.2:53893343-53893362	MS.gene027441:CDS	10.0%
!!!	TTTTAAATTCATAATTTCAA+TGG	+	chr4.2:53892843-53892862	None:intergenic	10.0%
!!	AACACTTAATAATATACTCT+TGG	+	chr4.2:53893468-53893487	None:intergenic	20.0%
!!	AGAAATCAAGAAAGATATAA+AGG	+	chr4.2:53893316-53893335	None:intergenic	20.0%
!!	TTCATAATTTCAATGGTAAA+CGG	+	chr4.2:53892836-53892855	None:intergenic	20.0%
!!!	AAGAGTATATTATTAAGTGT+TGG	-	chr4.2:53893467-53893486	MS.gene027441:CDS	20.0%
!!!	AGAAAGACACTATATATTTT+TGG	+	chr4.2:53892931-53892950	None:intergenic	20.0%
!	AAAATGGAATATTAAACCAG+AGG	-	chr4.2:53893203-53893222	MS.gene027441:intron	25.0%
!	AAATGGAATATTAAACCAGA+GGG	-	chr4.2:53893204-53893223	MS.gene027441:intron	25.0%
!	CATTAGAAGTAATATAACAG+AGG	-	chr4.2:53893589-53893608	MS.gene027441:CDS	25.0%
!	CGCTAAAATTGATACAAAAA+AGG	+	chr4.2:53893834-53893853	None:intergenic	25.0%
!	TCATAATTTCAATGGTAAAC+GGG	+	chr4.2:53892835-53892854	None:intergenic	25.0%
!	TGTTAAGAATCAACAATATG+AGG	-	chr4.2:53893120-53893139	MS.gene027441:CDS	25.0%
!	TTATTGAATAAGATTACCTG+CGG	+	chr4.2:53893277-53893296	None:intergenic	25.0%
!!	ATTTAAAACCTTCATTTGCT+TGG	-	chr4.2:53892855-53892874	MS.gene027441:CDS	25.0%
!!	TGACATAATAGTTCTTTTTG+AGG	+	chr4.2:53893555-53893574	None:intergenic	25.0%
	AAAGACCTTGTTAGAGTTAA+AGG	-	chr4.2:53892965-53892984	MS.gene027441:CDS	30.0%
	AATAATATACTCTTGGTCTC+TGG	+	chr4.2:53893461-53893480	None:intergenic	30.0%
	ATAGATGCAGCAATATGTAA+CGG	+	chr4.2:53892803-53892822	None:intergenic	30.0%
	ATATATGTTATGATACCAGC+TGG	-	chr4.2:53893854-53893873	MS.gene027441:CDS	30.0%
	TAGGGTAGAAATAGTAGAAT+TGG	+	chr4.2:53893744-53893763	None:intergenic	30.0%
	TTACATATTGCTGCATCTAT+GGG	-	chr4.2:53892803-53892822	MS.gene027441:intron	30.0%
!	AATTAATTTCTTGGTAGCAC+AGG	-	chr4.2:53893352-53893371	MS.gene027441:CDS	30.0%
!	CAACACCATTTTGCATATTT+CGG	-	chr4.2:53893237-53893256	MS.gene027441:intron	30.0%
!	CTTCATTTTGCAAGTCAAAA+TGG	-	chr4.2:53893007-53893026	MS.gene027441:CDS	30.0%
!!	AATTCTACTATTTCTACCCT+AGG	-	chr4.2:53893743-53893762	MS.gene027441:CDS	30.0%
!!	GGTAAGTACTGGTATAAATT+TGG	-	chr4.2:53893388-53893407	MS.gene027441:CDS	30.0%
!!!	TTTTGACTTGCAAAATGAAG+AGG	+	chr4.2:53893007-53893026	None:intergenic	30.0%
	AAATTTGGACGCAAAGAATG+AGG	-	chr4.2:53893403-53893422	MS.gene027441:CDS	35.0%
	CCAGCTAACAAGTATTTGAA+GGG	+	chr4.2:53893146-53893165	None:intergenic	35.0%
	CTCAAACGAAATAATCGAAG+AGG	-	chr4.2:53893166-53893185	MS.gene027441:CDS	35.0%
	CTCTTCCTTTAACTCTAACA+AGG	+	chr4.2:53892973-53892992	None:intergenic	35.0%
	GGAATTTCTCTTTCTTTGTC+CGG	-	chr4.2:53893048-53893067	MS.gene027441:CDS	35.0%
	GGTAGAAATAGTAGAATTGG+CGG	+	chr4.2:53893741-53893760	None:intergenic	35.0%
	GTTACATATTGCTGCATCTA+TGG	-	chr4.2:53892802-53892821	MS.gene027441:intron	35.0%
	GTTAGAGTTAAAGGAAGAGA+GGG	-	chr4.2:53892974-53892993	MS.gene027441:CDS	35.0%
	TAACCTGATAAATTCCACCA+GGG	+	chr4.2:53893685-53893704	None:intergenic	35.0%
	TCTATATGCTCCAGAATGAA+TGG	+	chr4.2:53892758-53892777	None:intergenic	35.0%
	TGTTAGAGTTAAAGGAAGAG+AGG	-	chr4.2:53892973-53892992	MS.gene027441:CDS	35.0%
!	CTTCTATTGTTGGTAAGTAC+TGG	-	chr4.2:53893377-53893396	MS.gene027441:CDS	35.0%
!	TAGTGCCGAAATATGCAAAA+TGG	+	chr4.2:53893245-53893264	None:intergenic	35.0%
!	TGTAACGGAGTTTCAACAAA+TGG	+	chr4.2:53892788-53892807	None:intergenic	35.0%
!	TTTAGCTTCCAAGCAAATGA+AGG	+	chr4.2:53892866-53892885	None:intergenic	35.0%
!	TTTTGCATAGCGAGATGAAT+GGG	+	chr4.2:53892905-53892924	None:intergenic	35.0%
	AACCTGATAAATTCCACCAG+GGG	+	chr4.2:53893684-53893703	None:intergenic	40.0%
	ACCTCACAGTCAAGTATTCA+AGG	+	chr4.2:53893078-53893097	None:intergenic	40.0%
	ATGATACCAGCTGGAATACT+AGG	-	chr4.2:53893863-53893882	MS.gene027441:CDS	40.0%
	ATTTCTACCCTAGGAAATGC+TGG	-	chr4.2:53893752-53893771	MS.gene027441:CDS	40.0%
	CCTCACAGTCAAGTATTCAA+GGG	+	chr4.2:53893077-53893096	None:intergenic	40.0%
	CTAACCTGATAAATTCCACC+AGG	+	chr4.2:53893686-53893705	None:intergenic	40.0%
	GAATCAGTGACTTCTAAGCT+CGG	+	chr4.2:53893500-53893519	None:intergenic	40.0%
	GCCAGCTAACAAGTATTTGA+AGG	+	chr4.2:53893147-53893166	None:intergenic	40.0%
	GGTGCTCAAAAGTTGGAAAA+TGG	-	chr4.2:53893187-53893206	MS.gene027441:CDS	40.0%
	GTCAAGTATTCAAGGGAATC+CGG	+	chr4.2:53893070-53893089	None:intergenic	40.0%
	GTTAGTGCCAGTGATGATAA+TGG	-	chr4.2:53893701-53893720	MS.gene027441:CDS	40.0%
	TTAAACCAGAGGGATAAAGC+AGG	-	chr4.2:53893214-53893233	MS.gene027441:intron	40.0%
	TTTCTACCCTAGGAAATGCT+GGG	-	chr4.2:53893753-53893772	MS.gene027441:CDS	40.0%
!	CCCTTCAAATACTTGTTAGC+TGG	-	chr4.2:53893143-53893162	MS.gene027441:CDS	40.0%
!	GTTTTGCATAGCGAGATGAA+TGG	+	chr4.2:53892906-53892925	None:intergenic	40.0%
!	TTTTGCAAGTCAAAATGGCG+AGG	-	chr4.2:53893012-53893031	MS.gene027441:CDS	40.0%
!!	AGTGCTTGCTATGTCTAATG+CGG	+	chr4.2:53893438-53893457	None:intergenic	40.0%
!!	TGGCGAGGTTGAATTTTTAG+CGG	-	chr4.2:53893027-53893046	MS.gene027441:CDS	40.0%
	ACCTGATAAATTCCACCAGG+GGG	+	chr4.2:53893683-53893702	None:intergenic	45.0%
	AGCACAGGCACTTCTATTGT+TGG	-	chr4.2:53893367-53893386	MS.gene027441:CDS	45.0%
	CCCTTGAATACTTGACTGTG+AGG	-	chr4.2:53893074-53893093	MS.gene027441:CDS	45.0%
	CCTAGGAAATGCTGGGAAAT+CGG	-	chr4.2:53893760-53893779	MS.gene027441:CDS	45.0%
	CGATTTCCCAGCATTTCCTA+GGG	+	chr4.2:53893762-53893781	None:intergenic	45.0%
	CGGAGATGATCCATTCATTC+TGG	-	chr4.2:53892745-53892764	MS.gene027441:intron	45.0%
	GATCTCCTCTACGCAGTAAT+CGG	-	chr4.2:53892725-53892744	MS.gene027441:intron	45.0%
	GCAACTGCGATCTATCAATC+CGG	-	chr4.2:53893650-53893669	MS.gene027441:CDS	45.0%
	GGAATACTAGGCTCTCTAGT+AGG	-	chr4.2:53893875-53893894	MS.gene027441:CDS	45.0%
	GTTCACGCCATTATCATCAC+TGG	+	chr4.2:53893711-53893730	None:intergenic	45.0%
	TCTCTAGTAGGTTCTCCCAT+GGG	-	chr4.2:53893887-53893906	MS.gene027441:CDS	45.0%
!!	AAGTCACTGATTCTCGCACA+CGG	-	chr4.2:53893507-53893526	MS.gene027441:CDS	45.0%
!!	TCGAAGAGGTGCTCAAAAGT+TGG	-	chr4.2:53893180-53893199	MS.gene027441:CDS	45.0%
	AGAGAGCCTAGTATTCCAGC+TGG	+	chr4.2:53893872-53893891	None:intergenic	50.0%
	CATCTCCGATTACTGCGTAG+AGG	+	chr4.2:53892733-53892752	None:intergenic	50.0%
	CCGATTTCCCAGCATTTCCT+AGG	+	chr4.2:53893763-53893782	None:intergenic	50.0%
	CTCTCTAGTAGGTTCTCCCA+TGG	-	chr4.2:53893886-53893905	MS.gene027441:CDS	50.0%
	GCTTGGAAGCTAAATCCGCA+GGG	-	chr4.2:53892872-53892891	MS.gene027441:CDS	50.0%
	TAGTAGGTTCTCCCATGGGT+TGG	-	chr4.2:53893891-53893910	MS.gene027441:CDS	50.0%
	TCCCCCTGGTGGAATTTATC+AGG	-	chr4.2:53893679-53893698	MS.gene027441:CDS	50.0%
	TGAATGGGACTGAATCCCTG+CGG	+	chr4.2:53892890-53892909	None:intergenic	50.0%
	TGCTTGGAAGCTAAATCCGC+AGG	-	chr4.2:53892871-53892890	MS.gene027441:CDS	50.0%
	TGTTGCCTGCTTTATCCCTC+TGG	+	chr4.2:53893222-53893241	None:intergenic	50.0%
!!	AGTCACTGATTCTCGCACAC+GGG	-	chr4.2:53893508-53893527	MS.gene027441:CDS	50.0%
!!	GCTGGGAAATCGGTTTTGTC+AGG	-	chr4.2:53893770-53893789	MS.gene027441:CDS	50.0%
	ATTCTCGCACACGGGCACAA+AGG	-	chr4.2:53893516-53893535	MS.gene027441:CDS	55.0%
	CAGAGGAGCAACGCAACACT+TGG	-	chr4.2:53893606-53893625	MS.gene027441:CDS	55.0%
	CTAGCGGCAAACCAACCCAT+GGG	+	chr4.2:53893905-53893924	None:intergenic	55.0%
	CTGAAAGCTGCAGCTCAGGA+AGG	-	chr4.2:53892695-53892714	MS.gene027441:CDS	55.0%
	CAATCCGGATTGAGTCCCCC+TGG	-	chr4.2:53893665-53893684	MS.gene027441:CDS	60.0%
	GCAGCTGAAAGCTGCAGCTC+AGG	-	chr4.2:53892691-53892710	MS.gene027441:CDS	60.0%
	GGCACTAAGTTGTGAGCCGC+AGG	-	chr4.2:53893258-53893277	MS.gene027441:intron	60.0%
	TCCACCAGGGGGACTCAATC+CGG	+	chr4.2:53893672-53893691	None:intergenic	60.0%
	GCACACGGGCACAAAGGCTC+AGG	-	chr4.2:53893522-53893541	MS.gene027441:CDS	65.0%
	TCCGGATTGAGTCCCCCTGG+TGG	-	chr4.2:53893668-53893687	MS.gene027441:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	53892671	53893924	53892671	ID=MS.gene027441
chr4.2	mRNA	53892671	53893924	53892671	ID=MS.gene027441.t1;Parent=MS.gene027441
chr4.2	exon	53893316	53893924	53893316	ID=MS.gene027441.t1.exon1;Parent=MS.gene027441.t1
chr4.2	CDS	53893316	53893924	53893316	ID=cds.MS.gene027441.t1;Parent=MS.gene027441.t1
chr4.2	exon	53892809	53893221	53892809	ID=MS.gene027441.t1.exon2;Parent=MS.gene027441.t1
chr4.2	CDS	53892809	53893221	53892809	ID=cds.MS.gene027441.t1;Parent=MS.gene027441.t1
chr4.2	exon	53892671	53892743	53892671	ID=MS.gene027441.t1.exon3;Parent=MS.gene027441.t1
chr4.2	CDS	53892671	53892743	53892671	ID=cds.MS.gene027441.t1;Parent=MS.gene027441.t1

Gene Sequence

>MS.gene027441

ATGAACACAAATATTTCTGAGCAGCTGAAAGCTGCAGCTCAGGAAGGTGATATAGATCTCCTCTACGCAGTAATCGGAGATGATCCATTCATTCTGGAGCATATAGATTTGATACCATTTGTTGAAACTCCGTTACATATTGCTGCATCTATGGGTCATATCCCGTTTACCATTGAAATTATGAATTTAAAACCTTCATTTGCTTGGAAGCTAAATCCGCAGGGATTCAGTCCCATTCATCTCGCTATGCAAAACGACCAAAAATATATAGTGTCTTTCTTTGTGCACAACAATAAAGACCTTGTTAGAGTTAAAGGAAGAGAGGGCTTAACTCCTCTTCATTTTGCAAGTCAAAATGGCGAGGTTGAATTTTTAGCGGAATTTCTCTTTCTTTGTCCGGATTCCCTTGAATACTTGACTGTGAGGTGCGAGACTGCACTGCATATTGCTGTTAAGAATCAACAATATGAGGCCCTTCAAATACTTGTTAGCTGGCTCAAACGAAATAATCGAAGAGGTGCTCAAAAGTTGGAAAATGGAATATTAAACCAGAGGGATAAAGCAGGCAACACCATTTTGCATATTTCGGCACTAAGTTGTGAGCCGCAGGTAATCTTATTCAATAACTAAAATCACATGTCTCCTTTATATCTTTCTTGATTTCTTGTATTGTTATTTGTTAATTAATTTCTTGGTAGCACAGGCACTTCTATTGTTGGTAAGTACTGGTATAAATTTGGACGCAAAGAATGAGGAGAACAAAACCGCATTAGACATAGCAAGCACTCCAGAGACCAAGAGTATATTATTAAGTGTTGGAGCAAAACCGAGCTTAGAAGTCACTGATTCTCGCACACGGGCACAAAGGCTCAGGTCAAAGACCTCAAAAAGAACTATTATGTCAACTTATGTAAATCGCATTAGAAGTAATATAACAGAGGAGCAACGCAACACTTGGTTGATAGTTTCAACTCTTGTTGCAACTGCGATCTATCAATCCGGATTGAGTCCCCCTGGTGGAATTTATCAGGTTAGTGCCAGTGATGATAATGGCGTGAACAACACTTCCGCCAATTCTACTATTTCTACCCTAGGAAATGCTGGGAAATCGGTTTTGTCAGGATATGAATTCTTTTTGTTTTTATTTATAAATATGTATTCCTTTTTTGTATCAATTTTAGCGATATATGTTATGATACCAGCTGGAATACTAGGCTCTCTAGTAGGTTCTCCCATGGGTTGGTTTGCCGCTAG

Protein sequence

>MS.gene027441.t1

MNTNISEQLKAAAQEGDIDLLYAVIGDDPFILEHIDLIPFVETPLHIAASMGHIPFTIEIMNLKPSFAWKLNPQGFSPIHLAMQNDQKYIVSFFVHNNKDLVRVKGREGLTPLHFASQNGEVEFLAEFLFLCPDSLEYLTVRCETALHIAVKNQQYEALQILVSWLKRNNRRGAQKLENGILNQRDKAGNTILHISALSCEPQALLLLVSTGINLDAKNEENKTALDIASTPETKSILLSVGAKPSLEVTDSRTRAQRLRSKTSKRTIMSTYVNRIRSNITEEQRNTWLIVSTLVATAIYQSGLSPPGGIYQVSASDDNGVNNTSANSTISTLGNAGKSVFDICYDTSWNTRLSSRFSHGLVCR