AlfalfaGEDB | MS.gene87218

M. sativa cultivar XinJiangDaYe / MS.gene87218

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF12796.10 PF13857.9 PF13962.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene87218.t1	AES77856.1	60.6	340	128	1	1	340	1	334	6.90E-96	360.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene87218.t1	Q8GYH5	37.7	302	179	3	8	302	4	303	1.1e-14	82.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene87218.t1	G7KSX2	60.6	340	128	1	1	340	1	334	4.9e-96	360.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene066501	MS.gene87218	PPI
MS.gene08470	MS.gene87218	PPI
MS.gene63085	MS.gene87218	PPI
MS.gene056455	MS.gene87218	PPI
MS.gene87218	MS.gene053363	PPI
MS.gene89986	MS.gene87218	PPI
MS.gene26019	MS.gene87218	PPI
MS.gene23881	MS.gene87218	PPI
MS.gene052956	MS.gene87218	PPI
MS.gene91775	MS.gene87218	PPI
MS.gene87218	MS.gene32726	PPI
MS.gene34009	MS.gene87218	PPI
MS.gene30177	MS.gene87218	PPI
MS.gene69965	MS.gene87218	PPI
MS.gene65463	MS.gene87218	PPI
MS.gene066500	MS.gene87218	PPI
MS.gene87218	MS.gene60920	PPI
MS.gene87218	MS.gene92243	PPI
MS.gene21888	MS.gene87218	PPI
MS.gene82740	MS.gene87218	PPI
MS.gene042783	MS.gene87218	PPI
MS.gene09785	MS.gene87218	PPI
MS.gene54764	MS.gene87218	PPI
MS.gene30172	MS.gene87218	PPI
MS.gene045937	MS.gene87218	PPI
MS.gene51278	MS.gene87218	PPI
MS.gene23852	MS.gene87218	PPI
MS.gene97943	MS.gene87218	PPI
MS.gene97379	MS.gene87218	PPI
MS.gene67157	MS.gene87218	PPI
MS.gene99973	MS.gene87218	PPI
MS.gene65775	MS.gene87218	PPI
MS.gene30433	MS.gene87218	PPI
MS.gene012203	MS.gene87218	PPI
MS.gene022109	MS.gene87218	PPI
MS.gene34651	MS.gene87218	PPI
MS.gene25244	MS.gene87218	PPI
MS.gene014931	MS.gene87218	PPI
MS.gene010910	MS.gene87218	PPI
MS.gene87218	MS.gene32723	PPI
MS.gene32726	MS.gene87218	PPI
MS.gene025229	MS.gene87218	PPI
MS.gene063222	MS.gene87218	PPI
MS.gene25515	MS.gene87218	PPI
MS.gene30174	MS.gene87218	PPI
MS.gene062662	MS.gene87218	PPI
MS.gene73119	MS.gene87218	PPI
MS.gene69687	MS.gene87218	PPI
MS.gene035805	MS.gene87218	PPI
MS.gene87218	MS.gene71122	PPI
MS.gene87218	MS.gene51278	PPI
MS.gene51258	MS.gene87218	PPI
MS.gene23810	MS.gene87218	PPI
MS.gene024753	MS.gene87218	PPI
MS.gene87218	MS.gene23881	PPI
MS.gene78281	MS.gene87218	PPI
MS.gene005995	MS.gene87218	PPI
MS.gene037950	MS.gene87218	PPI
MS.gene70008	MS.gene87218	PPI
MS.gene64441	MS.gene87218	PPI
MS.gene69966	MS.gene87218	PPI
MS.gene24806	MS.gene87218	PPI
MS.gene071113	MS.gene87218	PPI
MS.gene004844	MS.gene87218	PPI
MS.gene30176	MS.gene87218	PPI
MS.gene027071	MS.gene87218	PPI
MS.gene32723	MS.gene87218	PPI
MS.gene007487	MS.gene87218	PPI
MS.gene007485	MS.gene87218	PPI
MS.gene018518	MS.gene87218	PPI
MS.gene037184	MS.gene87218	PPI
MS.gene066464	MS.gene87218	PPI
MS.gene87218	MS.gene65775	PPI
MS.gene87218	MS.gene80163	PPI
MS.gene067705	MS.gene87218	PPI
MS.gene26021	MS.gene87218	PPI
MS.gene07706	MS.gene87218	PPI
MS.gene027074	MS.gene87218	PPI
MS.gene51262	MS.gene87218	PPI
MS.gene87218	MS.gene027072	PPI
MS.gene90400	MS.gene87218	PPI
MS.gene050270	MS.gene87218	PPI
MS.gene02469	MS.gene87218	PPI
MS.gene008833	MS.gene87218	PPI
MS.gene24922	MS.gene87218	PPI
MS.gene010912	MS.gene87218	PPI
MS.gene050457	MS.gene87218	PPI
MS.gene25024	MS.gene87218	PPI
MS.gene23811	MS.gene87218	PPI
MS.gene34712	MS.gene87218	PPI
MS.gene51335	MS.gene87218	PPI
MS.gene39623	MS.gene87218	PPI
MS.gene065848	MS.gene87218	PPI
MS.gene027441	MS.gene87218	PPI
MS.gene87218	MS.gene99973	PPI
MS.gene068249	MS.gene87218	PPI
MS.gene066046	MS.gene87218	PPI
MS.gene051003	MS.gene87218	PPI
MS.gene87218	MS.gene008833	PPI
MS.gene27223	MS.gene87218	PPI
MS.gene038519	MS.gene87218	PPI
MS.gene050272	MS.gene87218	PPI
MS.gene81055	MS.gene87218	PPI
MS.gene87218	MS.gene48655	PPI
MS.gene22482	MS.gene87218	PPI
MS.gene043662	MS.gene87218	PPI
MS.gene039682	MS.gene87218	PPI
MS.gene043742	MS.gene87218	PPI
MS.gene061932	MS.gene87218	PPI
MS.gene48655	MS.gene87218	PPI
MS.gene85534	MS.gene87218	PPI
MS.gene027073	MS.gene87218	PPI
MS.gene26532	MS.gene87218	PPI
MS.gene064542	MS.gene87218	PPI
MS.gene066467	MS.gene87218	PPI
MS.gene47479	MS.gene87218	PPI
MS.gene21734	MS.gene87218	PPI
MS.gene33377	MS.gene87218	PPI
MS.gene008767	MS.gene87218	PPI
MS.gene23196	MS.gene87218	PPI
MS.gene71204	MS.gene87218	PPI
MS.gene87218	MS.gene067705	PPI
MS.gene007486	MS.gene87218	PPI
MS.gene80163	MS.gene87218	PPI
MS.gene041770	MS.gene87218	PPI
MS.gene71122	MS.gene87218	PPI
MS.gene26672	MS.gene87218	PPI
MS.gene36842	MS.gene87218	PPI
MS.gene99554	MS.gene87218	PPI
MS.gene050456	MS.gene87218	PPI
MS.gene058128	MS.gene87218	PPI
MS.gene99588	MS.gene87218	PPI
MS.gene027075	MS.gene87218	PPI
MS.gene41291	MS.gene87218	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene87218.t1	MTR_8g072510	76.696	339	78	1	3	341	4	341	0.0	518
MS.gene87218.t1	MTR_7g021090	71.848	341	90	1	1	341	1	335	1.28e-173	489
MS.gene87218.t1	MTR_8g039620	70.201	349	92	3	7	344	12	359	3.53e-171	484
MS.gene87218.t1	MTR_8g062410	70.085	351	75	3	3	341	4	336	2.56e-164	467
MS.gene87218.t1	MTR_1g059930	66.570	344	112	2	1	341	1	344	3.34e-159	454
MS.gene87218.t1	MTR_3g016210	65.642	358	107	3	3	345	4	360	7.20e-158	451
MS.gene87218.t1	MTR_8g068990	62.295	366	112	7	1	357	1	349	3.32e-151	434
MS.gene87218.t1	MTR_8g073260	57.480	381	132	5	1	357	1	375	4.32e-148	426
MS.gene87218.t1	MTR_7g451420	67.308	312	102	0	1	312	1	312	1.34e-145	414
MS.gene87218.t1	MTR_8g069880	61.850	346	126	4	9	350	7	350	6.61e-137	398
MS.gene87218.t1	MTR_8g040930	66.216	296	90	4	51	340	1	292	5.34e-135	390
MS.gene87218.t1	MTR_8g041240	66.216	296	90	4	51	340	1	292	5.34e-135	390
MS.gene87218.t1	MTR_8g066670	61.696	342	128	3	1	341	1067	1406	3.24e-127	398
MS.gene87218.t1	MTR_8g467780	57.681	345	136	2	7	341	9	353	5.17e-127	372
MS.gene87218.t1	MTR_3g105650	50.968	310	144	2	7	309	3	311	2.18e-103	311
MS.gene87218.t1	MTR_8g073275	44.972	358	121	7	51	342	1	348	1.52e-83	261
MS.gene87218.t1	MTR_7g099580	27.010	311	176	9	7	312	10	274	5.36e-26	107
MS.gene87218.t1	MTR_1g069110	26.316	323	210	7	9	325	18	318	1.01e-25	107
MS.gene87218.t1	MTR_7g099570	29.302	215	138	3	1	210	1	206	6.63e-21	94.7
MS.gene87218.t1	MTR_2g101320	28.378	222	137	4	37	248	134	343	1.38e-20	93.6
MS.gene87218.t1	MTR_7g117620	26.370	292	171	6	44	308	119	393	2.64e-20	92.4
MS.gene87218.t1	MTR_1g115575	26.460	291	175	6	44	309	169	445	3.77e-19	89.4
MS.gene87218.t1	MTR_2g096190	25.000	288	180	6	45	308	105	380	6.07e-18	85.5
MS.gene87218.t1	MTR_2g101310	27.572	243	152	4	40	268	137	369	1.44e-17	84.7
MS.gene87218.t1	MTR_2g101310	27.572	243	152	4	40	268	137	369	1.59e-17	84.0
MS.gene87218.t1	MTR_4g062100	25.000	288	180	6	45	308	109	384	7.72e-17	82.0
MS.gene87218.t1	MTR_6g027840	30.102	196	125	6	44	230	246	438	4.50e-16	79.7
MS.gene87218.t1	MTR_7g100390	25.000	288	181	6	44	308	87	362	6.92e-15	76.3
MS.gene87218.t1	MTR_1g032100	24.828	290	181	7	44	308	94	371	2.51e-14	74.3
MS.gene87218.t1	MTR_8g105260	24.138	290	183	6	44	308	101	378	4.53e-14	73.6
MS.gene87218.t1	MTR_7g100430	24.620	329	190	11	42	321	124	443	1.50e-13	72.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene87218.t1	AT4G10720	41.562	320	179	2	8	319	4	323	1.87e-80	252
MS.gene87218.t1	AT4G10720	41.562	320	179	2	8	319	4	323	4.44e-80	252
MS.gene87218.t1	AT5G15500	39.710	345	189	5	9	345	6	339	2.09e-74	238
MS.gene87218.t1	AT1G14500	37.898	314	186	2	8	312	4	317	4.39e-74	236
MS.gene87218.t1	AT1G14480	38.080	323	190	2	8	320	4	326	2.87e-73	234
MS.gene87218.t1	AT5G54620	39.088	307	180	2	7	306	3	309	6.48e-70	225
MS.gene87218.t1	AT5G54610	34.653	303	189	3	8	303	4	304	3.76e-60	200
MS.gene87218.t1	AT1G14480	36.624	314	164	4	8	320	4	283	2.72e-57	191
MS.gene87218.t1	AT5G15500	35.246	244	139	5	110	345	1	233	2.91e-34	129
MS.gene87218.t1	AT5G51160	30.292	274	163	4	63	315	35	301	9.43e-32	124
MS.gene87218.t1	AT1G03670	31.429	245	119	9	88	315	266	478	7.07e-20	91.7
MS.gene87218.t1	AT1G34050	26.538	260	169	5	65	312	174	423	2.06e-19	90.1
MS.gene87218.t1	AT4G03500	25.402	311	163	8	43	309	217	502	6.11e-19	89.0
MS.gene87218.t1	AT4G03500	25.402	311	163	8	43	309	194	479	7.50e-19	88.6
MS.gene87218.t1	AT4G03500	25.402	311	163	8	43	309	194	479	7.50e-19	88.6
MS.gene87218.t1	AT4G03460	30.909	220	119	6	100	309	258	454	2.31e-18	87.0
MS.gene87218.t1	AT4G03460	30.909	220	119	6	100	309	290	486	2.49e-18	87.0
MS.gene87218.t1	AT2G01680	24.915	293	183	6	44	311	97	377	2.83e-17	83.6
MS.gene87218.t1	AT5G54700	29.515	227	120	7	95	316	17	208	7.92e-17	81.3
MS.gene87218.t1	AT3G09550	25.369	339	199	10	12	321	157	470	3.06e-16	80.5
MS.gene87218.t1	AT3G09550	25.369	339	199	10	12	321	150	463	3.09e-16	80.5
MS.gene87218.t1	AT3G09550	25.369	339	199	10	12	321	150	463	3.09e-16	80.5
MS.gene87218.t1	AT1G05640	24.079	353	181	11	42	308	114	465	8.30e-16	79.3
MS.gene87218.t1	AT3G12360	25.085	295	174	7	44	309	155	431	1.91e-15	78.2
MS.gene87218.t1	AT3G12360	25.085	295	174	7	44	309	167	443	2.05e-15	78.2
MS.gene87218.t1	AT1G10340	28.395	243	120	6	109	312	205	432	2.17e-15	77.8
MS.gene87218.t1	AT1G10340	28.395	243	120	6	109	312	201	428	2.45e-15	77.8
MS.gene87218.t1	AT5G54710	26.667	240	141	6	98	312	267	496	1.27e-14	75.5
MS.gene87218.t1	AT5G54710	26.667	240	141	6	98	312	222	451	1.33e-14	75.5
MS.gene87218.t1	AT1G07710	24.768	323	207	8	9	308	70	379	1.58e-14	75.1
MS.gene87218.t1	AT1G07710	24.768	323	207	8	9	308	70	379	1.58e-14	75.1
MS.gene87218.t1	AT2G24600	26.708	322	185	10	30	312	123	432	1.89e-14	75.1
MS.gene87218.t1	AT2G24600	26.708	322	185	10	30	312	123	432	1.89e-14	75.1
MS.gene87218.t1	AT2G24600	26.374	364	205	12	30	353	123	463	2.49e-14	74.7
MS.gene87218.t1	AT2G24600	26.374	364	205	12	30	353	123	463	2.49e-14	74.7
MS.gene87218.t1	AT5G54700	25.993	277	134	9	42	316	38	245	2.60e-14	74.3
MS.gene87218.t1	AT5G60070	23.208	293	184	8	44	308	111	390	1.27e-13	72.4
MS.gene87218.t1	AT2G31820	25.000	288	180	8	44	308	227	501	1.37e-13	72.4
MS.gene87218.t1	AT4G03450	25.121	207	140	4	108	310	290	485	1.59e-13	72.4
MS.gene87218.t1	AT4G03450	25.121	207	140	4	108	310	290	485	1.68e-13	72.0
MS.gene87218.t1	AT4G03490	26.214	309	168	10	9	309	211	467	4.11e-13	70.9
MS.gene87218.t1	AT4G03490	26.214	309	168	10	9	309	201	457	4.22e-13	70.9
MS.gene87218.t1	AT4G03490	26.214	309	168	10	9	309	211	467	4.49e-13	70.9
MS.gene87218.t1	AT5G02620	23.024	291	185	6	45	308	95	373	9.51e-13	69.7
MS.gene87218.t1	AT5G02620	23.024	291	185	6	45	308	95	373	9.51e-13	69.7
MS.gene87218.t1	AT5G02620	23.024	291	185	6	45	308	95	373	9.51e-13	69.7
MS.gene87218.t1	AT5G50140	28.902	173	98	4	40	197	26	188	1.88e-12	68.9
MS.gene87218.t1	AT4G03480	25.000	324	184	12	45	347	357	642	6.85e-12	67.4
MS.gene87218.t1	AT4G03480	25.000	324	184	12	45	347	360	645	7.19e-12	67.0
MS.gene87218.t1	AT4G14390	23.962	313	206	7	9	310	233	524	2.26e-11	65.9
MS.gene87218.t1	AT4G14390	23.962	313	206	7	9	310	252	543	2.67e-11	65.5
MS.gene87218.t1	AT4G14390	23.885	314	206	7	9	310	252	544	3.22e-11	65.1
MS.gene87218.t1	AT4G05040	25.879	313	200	10	9	310	235	526	8.67e-11	63.9
MS.gene87218.t1	AT4G05040	25.879	313	200	10	9	310	235	526	8.67e-11	63.9
MS.gene87218.t1	AT4G05040	25.879	313	200	10	9	310	235	526	8.67e-11	63.9
MS.gene87218.t1	AT4G05040	25.879	313	200	10	9	310	235	526	8.67e-11	63.9
MS.gene87218.t1	AT4G05040	25.879	313	200	10	9	310	235	526	8.67e-11	63.9
MS.gene87218.t1	AT4G05040	25.879	313	200	10	9	310	235	526	8.67e-11	63.9
MS.gene87218.t1	AT4G05040	25.879	313	200	10	9	310	235	526	8.67e-11	63.9

Find 70 sgRNAs with CRISPR-Local

Find 92 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGACCTTGTTAGAGTTAA+AGG	0.117136	4.4:-54153268	MS.gene87218:CDS
GGTAAGTACTGGTATAAATT+TGG	0.243776	4.4:-54152845	MS.gene87218:CDS
GTTACATATTGCTGCATCTA+TGG	0.262404	4.4:-54153431	MS.gene87218:CDS
GGTGCTCAAAAGTTGGAAAA+TGG	0.312014	4.4:-54153046	MS.gene87218:CDS
CGGAGATGATCCATTCATTC+TGG	0.324894	4.4:-54153488	MS.gene87218:CDS
TAGTGCCGAAATATGCAAAA+TGG	0.358761	4.4:+54152991	None:intergenic
TAGGGTAGAAATAGTAGAAT+TGG	0.371125	4.4:+54152492	None:intergenic
GCAGCTGAAAGCTGCAGCTC+AGG	0.395348	4.4:-54153542	MS.gene87218:CDS
CTCTCTAGTTGGTTCTCCGA+TGG	0.406115	4.4:-54152347	MS.gene87218:CDS
TGTTGCCTGCTTTATCCCTC+TGG	0.417230	4.4:+54153014	None:intergenic
ATTTAAAACCTTCATTTGCT+TGG	0.418134	4.4:-54153378	MS.gene87218:CDS
GATCTCCTCTATGCAGTAAT+CGG	0.419361	4.4:-54153508	MS.gene87218:CDS
GGAATTTCTCTTTCTTTGTC+CGG	0.421807	4.4:-54153185	MS.gene87218:CDS
CTTCTATTGTTGGTAAGTAC+TGG	0.432315	4.4:-54152856	MS.gene87218:CDS
CCCTTCAAATACTTGTTAGC+TGG	0.445898	4.4:-54153090	MS.gene87218:CDS
TTTATATGCTCCAGAATGAA+TGG	0.446072	4.4:+54153478	None:intergenic
ATGATACCAGTTGGAATACT+AGG	0.455258	4.4:-54152370	MS.gene87218:CDS
AGCACAGGCACTTCTATTGT+TGG	0.470486	4.4:-54152866	MS.gene87218:intron
GTCAAGTATTCAAGGGAATC+CGG	0.474006	4.4:+54153166	None:intergenic
TCGAAGAGGTGCTCAAAAGT+TGG	0.483334	4.4:-54153053	MS.gene87218:CDS
AATAATATACTCTTGATCTC+TGG	0.484204	4.4:+54152775	None:intergenic
ATTTCTACCCTAGAAAATGC+TGG	0.485862	4.4:-54152481	MS.gene87218:CDS
TCTCTAGTTGGTTCTCCGAT+GGG	0.491549	4.4:-54152346	MS.gene87218:CDS
TGTAACGGAGTTTCAACAAA+TGG	0.492054	4.4:+54153448	None:intergenic
TTTAGCTTCCAAGCAAATGA+AGG	0.492400	4.4:+54153370	None:intergenic
GCCAGCTAACAAGTATTTGA+AGG	0.497511	4.4:+54153089	None:intergenic
TGCTTGGAAGCTAAATCCGC+AGG	0.508838	4.4:-54153362	MS.gene87218:CDS
TGTTAAGAATCAACAATATG+AGG	0.510561	4.4:-54153113	MS.gene87218:CDS
AGAGAGCCTAGTATTCCAAC+TGG	0.516739	4.4:+54152364	None:intergenic
ACCTCACAGTCAAGTATTCA+AGG	0.520683	4.4:+54153158	None:intergenic
GTTAGAGTTAAAGGAAGAGA+GGG	0.523986	4.4:-54153259	MS.gene87218:CDS
AAGAGTATATTATTAAGTGT+TGG	0.527914	4.4:-54152766	MS.gene87218:CDS
GGAATACTAGGCTCTCTAGT+TGG	0.530906	4.4:-54152358	MS.gene87218:CDS
TTACATATTGCTGCATCTAT+GGG	0.532476	4.4:-54153430	MS.gene87218:CDS
GTTCACGCCATTGTCATCAC+TGG	0.534262	4.4:+54152525	None:intergenic
TAGTTGGTTCTCCGATGGGT+TGG	0.537418	4.4:-54152342	MS.gene87218:CDS
CAGAGGAGCAACGCAACACT+TGG	0.546199	4.4:-54152627	MS.gene87218:CDS
GCAACTGCGATCTATCAATC+CGG	0.547636	4.4:-54152583	MS.gene87218:CDS
GCACACGGGCACAAAGGCTC+AGG	0.547763	4.4:-54152711	MS.gene87218:CDS
GGTAGAAATAGTAGAATTGG+CGG	0.556035	4.4:+54152495	None:intergenic
CTGAAAGCTGCAGCTCAGGA+AGG	0.558773	4.4:-54153538	MS.gene87218:CDS
TTTCTACCCTAGAAAATGCT+GGG	0.560140	4.4:-54152480	MS.gene87218:CDS
CTCTTCCTTTAACTCTAACA+AGG	0.564990	4.4:+54153263	None:intergenic
TGTTAGAGTTAAAGGAAGAG+AGG	0.566339	4.4:-54153260	MS.gene87218:CDS
GGCACTAAATAGTGAGCCGC+AGG	0.567544	4.4:-54152975	MS.gene87218:intron
AAATGGAATATTAAACCAGA+GGG	0.585975	4.4:-54153029	MS.gene87218:CDS
ATATATGTTATGATACCAGT+TGG	0.589290	4.4:-54152379	MS.gene87218:CDS
CTAACCTGATAAATTCCACC+AGG	0.592230	4.4:+54152550	None:intergenic
AAATTTGGACGCAAAGAATG+AGG	0.593661	4.4:-54152830	MS.gene87218:CDS
CCAGCTAACAAGTATTTGAA+GGG	0.614265	4.4:+54153090	None:intergenic
CCCTTGAATACTTGACTGTG+AGG	0.615568	4.4:-54153159	MS.gene87218:CDS
TTAAACCAGAGGGATAAAGC+AGG	0.617620	4.4:-54153019	MS.gene87218:CDS
CATCTCCGATTACTGCATAG+AGG	0.617800	4.4:+54153503	None:intergenic
GAATCAGTGACTTCTAAGCT+CGG	0.618191	4.4:+54152736	None:intergenic
ATTCTCGCACACGGGCACAA+AGG	0.624210	4.4:-54152717	MS.gene87218:CDS
AAGTCACTGATTCTCGCACA+CGG	0.625915	4.4:-54152726	MS.gene87218:CDS
CCTCACAGTCAAGTATTCAA+GGG	0.642621	4.4:+54153159	None:intergenic
CTCAAACGAAATAATCGAAG+AGG	0.644802	4.4:-54153067	MS.gene87218:CDS
ATAGATGCAGCAATATGTAA+CGG	0.652232	4.4:+54153433	None:intergenic
GCTTGGAAGCTAAATCCGCA+GGG	0.653006	4.4:-54153361	MS.gene87218:CDS
AAAATGGAATATTAAACCAG+AGG	0.659194	4.4:-54153030	MS.gene87218:CDS
AGTCACTGATTCTCGCACAC+GGG	0.663891	4.4:-54152725	MS.gene87218:CDS
AGTGCTTGCTATGTCTAATG+CGG	0.681177	4.4:+54152798	None:intergenic
CTAGCGGCAAACCAACCCAT+CGG	0.681231	4.4:+54152331	None:intergenic
GTTAGTGCCAGTGATGACAA+TGG	0.683840	4.4:-54152532	MS.gene87218:CDS
AACCTGATAAATTCCACCAG+GGG	0.693447	4.4:+54152552	None:intergenic
TAACCTGATAAATTCCACCA+GGG	0.695982	4.4:+54152551	None:intergenic
ACCTGATAAATTCCACCAGG+GGG	0.706697	4.4:+54152553	None:intergenic
CATTAGAAGTAATATAACAG+AGG	0.709657	4.4:-54152644	MS.gene87218:CDS
TGAATGGGACTGAATCCCTG+CGG	0.749640	4.4:+54153346	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTATTTGTTAATTAATTTCT+TGG	-	chr4.4:54153003-54153022	MS.gene87218:CDS	10.0%
!!!	TTTTAAATTCATAATTTCAA+TGG	+	chr4.4:54152503-54152522	None:intergenic	10.0%
!!	AGAAATCAAGAAAGATATAA+AGG	+	chr4.4:54152976-54152995	None:intergenic	20.0%
!!!	AAGAGTATATTATTAAGTGT+TGG	-	chr4.4:54153127-54153146	MS.gene87218:CDS	20.0%
!!!	AGAAAGACACTATATATTTT+TGG	+	chr4.4:54152591-54152610	None:intergenic	20.0%
!	AAAATGGAATATTAAACCAG+AGG	-	chr4.4:54152863-54152882	MS.gene87218:intron	25.0%
!	AAATGGAATATTAAACCAGA+GGG	-	chr4.4:54152864-54152883	MS.gene87218:intron	25.0%
!	AATAATATACTCTTGATCTC+TGG	+	chr4.4:54153121-54153140	None:intergenic	25.0%
!	ATATATGTTATGATACCAGT+TGG	-	chr4.4:54153514-54153533	MS.gene87218:CDS	25.0%
!	CATTAGAAGTAATATAACAG+AGG	-	chr4.4:54153249-54153268	MS.gene87218:CDS	25.0%
!	CGCTAAAATTGATACAAAAA+AGG	+	chr4.4:54153494-54153513	None:intergenic	25.0%
!	TGTTAAGAATCAACAATATG+AGG	-	chr4.4:54152780-54152799	MS.gene87218:CDS	25.0%
!	TTATTGAATAAGATTACCTG+CGG	+	chr4.4:54152937-54152956	None:intergenic	25.0%
!!	ATTTAAAACCTTCATTTGCT+TGG	-	chr4.4:54152515-54152534	MS.gene87218:CDS	25.0%
!!	TGACATAATAGTTCTTTTTG+AGG	+	chr4.4:54153215-54153234	None:intergenic	25.0%
	AAAGACCTTGTTAGAGTTAA+AGG	-	chr4.4:54152625-54152644	MS.gene87218:CDS	30.0%
	ATAGATGCAGCAATATGTAA+CGG	+	chr4.4:54152463-54152482	None:intergenic	30.0%
	TAGGGTAGAAATAGTAGAAT+TGG	+	chr4.4:54153404-54153423	None:intergenic	30.0%
	TTACATATTGCTGCATCTAT+GGG	-	chr4.4:54152463-54152482	MS.gene87218:intron	30.0%
	TTTATATGCTCCAGAATGAA+TGG	+	chr4.4:54152418-54152437	None:intergenic	30.0%
!	AATTAATTTCTTGGTAGCAC+AGG	-	chr4.4:54153012-54153031	MS.gene87218:CDS	30.0%
!	CAACACCATTTTGCATATTT+CGG	-	chr4.4:54152897-54152916	MS.gene87218:intron	30.0%
!	CTTCATTTTGCAAGTCAAAA+TGG	-	chr4.4:54152667-54152686	MS.gene87218:CDS	30.0%
!!	GGTAAGTACTGGTATAAATT+TGG	-	chr4.4:54153048-54153067	MS.gene87218:CDS	30.0%
!!!	TTTTGACTTGCAAAATGAAG+AGG	+	chr4.4:54152667-54152686	None:intergenic	30.0%
	AAATTTGGACGCAAAGAATG+AGG	-	chr4.4:54153063-54153082	MS.gene87218:CDS	35.0%
	ATGATACCAGTTGGAATACT+AGG	-	chr4.4:54153523-54153542	MS.gene87218:CDS	35.0%
	ATTTCTACCCTAGAAAATGC+TGG	-	chr4.4:54153412-54153431	MS.gene87218:CDS	35.0%
	CCAGCTAACAAGTATTTGAA+GGG	+	chr4.4:54152806-54152825	None:intergenic	35.0%
	CTCAAACGAAATAATCGAAG+AGG	-	chr4.4:54152826-54152845	MS.gene87218:CDS	35.0%
	CTCTTCCTTTAACTCTAACA+AGG	+	chr4.4:54152633-54152652	None:intergenic	35.0%
	GGAATTTCTCTTTCTTTGTC+CGG	-	chr4.4:54152708-54152727	MS.gene87218:CDS	35.0%
	GGTAGAAATAGTAGAATTGG+CGG	+	chr4.4:54153401-54153420	None:intergenic	35.0%
	GTTACATATTGCTGCATCTA+TGG	-	chr4.4:54152462-54152481	MS.gene87218:intron	35.0%
	GTTAGAGTTAAAGGAAGAGA+GGG	-	chr4.4:54152634-54152653	MS.gene87218:CDS	35.0%
	TAACCTGATAAATTCCACCA+GGG	+	chr4.4:54153345-54153364	None:intergenic	35.0%
	TGTTAGAGTTAAAGGAAGAG+AGG	-	chr4.4:54152633-54152652	MS.gene87218:CDS	35.0%
	TTTCTACCCTAGAAAATGCT+GGG	-	chr4.4:54153413-54153432	MS.gene87218:CDS	35.0%
!	CTTCTATTGTTGGTAAGTAC+TGG	-	chr4.4:54153037-54153056	MS.gene87218:CDS	35.0%
!	TAGTGCCGAAATATGCAAAA+TGG	+	chr4.4:54152905-54152924	None:intergenic	35.0%
!	TGATTTCCCAGCATTTTCTA+GGG	+	chr4.4:54153422-54153441	None:intergenic	35.0%
!	TGTAACGGAGTTTCAACAAA+TGG	+	chr4.4:54152448-54152467	None:intergenic	35.0%
!	TTTAGCTTCCAAGCAAATGA+AGG	+	chr4.4:54152526-54152545	None:intergenic	35.0%
!	TTTTGCATAGCGAGATGAAT+GGG	+	chr4.4:54152565-54152584	None:intergenic	35.0%
	AACCTGATAAATTCCACCAG+GGG	+	chr4.4:54153344-54153363	None:intergenic	40.0%
	ACCTCACAGTCAAGTATTCA+AGG	+	chr4.4:54152738-54152757	None:intergenic	40.0%
	CCTCACAGTCAAGTATTCAA+GGG	+	chr4.4:54152737-54152756	None:intergenic	40.0%
	CTAACCTGATAAATTCCACC+AGG	+	chr4.4:54153346-54153365	None:intergenic	40.0%
	GAATCAGTGACTTCTAAGCT+CGG	+	chr4.4:54153160-54153179	None:intergenic	40.0%
	GATCTCCTCTATGCAGTAAT+CGG	-	chr4.4:54152385-54152404	MS.gene87218:intron	40.0%
	GCCAGCTAACAAGTATTTGA+AGG	+	chr4.4:54152807-54152826	None:intergenic	40.0%
	GGTGCTCAAAAGTTGGAAAA+TGG	-	chr4.4:54152847-54152866	MS.gene87218:CDS	40.0%
	GTCAAGTATTCAAGGGAATC+CGG	+	chr4.4:54152730-54152749	None:intergenic	40.0%
	TTAAACCAGAGGGATAAAGC+AGG	-	chr4.4:54152874-54152893	MS.gene87218:intron	40.0%
!	CCCTTCAAATACTTGTTAGC+TGG	-	chr4.4:54152803-54152822	MS.gene87218:CDS	40.0%
!	CTGATTTCCCAGCATTTTCT+AGG	+	chr4.4:54153423-54153442	None:intergenic	40.0%
!	GTTTTGCATAGCGAGATGAA+TGG	+	chr4.4:54152566-54152585	None:intergenic	40.0%
!	TTTTGCAAGTCAAAATGGCG+AGG	-	chr4.4:54152672-54152691	MS.gene87218:CDS	40.0%
!!	AGTGCTTGCTATGTCTAATG+CGG	+	chr4.4:54153098-54153117	None:intergenic	40.0%
!!	TGGCGAGGTTGAATTTTTAG+CGG	-	chr4.4:54152687-54152706	MS.gene87218:CDS	40.0%
	ACCTGATAAATTCCACCAGG+GGG	+	chr4.4:54153343-54153362	None:intergenic	45.0%
	AGAGAGCCTAGTATTCCAAC+TGG	+	chr4.4:54153532-54153551	None:intergenic	45.0%
	AGCACAGGCACTTCTATTGT+TGG	-	chr4.4:54153027-54153046	MS.gene87218:CDS	45.0%
	CATCTCCGATTACTGCATAG+AGG	+	chr4.4:54152393-54152412	None:intergenic	45.0%
	CCCTTGAATACTTGACTGTG+AGG	-	chr4.4:54152734-54152753	MS.gene87218:CDS	45.0%
	CGGAGATGATCCATTCATTC+TGG	-	chr4.4:54152405-54152424	MS.gene87218:intron	45.0%
	GCAACTGCGATCTATCAATC+CGG	-	chr4.4:54153310-54153329	MS.gene87218:CDS	45.0%
	GGAATACTAGGCTCTCTAGT+TGG	-	chr4.4:54153535-54153554	MS.gene87218:CDS	45.0%
	GTTAGTGCCAGTGATGACAA+TGG	-	chr4.4:54153361-54153380	MS.gene87218:CDS	45.0%
!	TCTCTAGTTGGTTCTCCGAT+GGG	-	chr4.4:54153547-54153566	MS.gene87218:CDS	45.0%
!!	AAGTCACTGATTCTCGCACA+CGG	-	chr4.4:54153167-54153186	MS.gene87218:CDS	45.0%
!!	GCTGGGAAATCAGTTTTGTC+AGG	-	chr4.4:54153430-54153449	MS.gene87218:CDS	45.0%
!!	TCGAAGAGGTGCTCAAAAGT+TGG	-	chr4.4:54152840-54152859	MS.gene87218:CDS	45.0%
	GCTTGGAAGCTAAATCCGCA+GGG	-	chr4.4:54152532-54152551	MS.gene87218:CDS	50.0%
	GTTCACGCCATTGTCATCAC+TGG	+	chr4.4:54153371-54153390	None:intergenic	50.0%
	TCCCCCTGGTGGAATTTATC+AGG	-	chr4.4:54153339-54153358	MS.gene87218:CDS	50.0%
	TGAATGGGACTGAATCCCTG+CGG	+	chr4.4:54152550-54152569	None:intergenic	50.0%
	TGCTTGGAAGCTAAATCCGC+AGG	-	chr4.4:54152531-54152550	MS.gene87218:CDS	50.0%
	TGTTGCCTGCTTTATCCCTC+TGG	+	chr4.4:54152882-54152901	None:intergenic	50.0%
!	CTCTCTAGTTGGTTCTCCGA+TGG	-	chr4.4:54153546-54153565	MS.gene87218:CDS	50.0%
!	TAGTTGGTTCTCCGATGGGT+TGG	-	chr4.4:54153551-54153570	MS.gene87218:CDS	50.0%
!!	AGTCACTGATTCTCGCACAC+GGG	-	chr4.4:54153168-54153187	MS.gene87218:CDS	50.0%
	ATTCTCGCACACGGGCACAA+AGG	-	chr4.4:54153176-54153195	MS.gene87218:CDS	55.0%
	CAGAGGAGCAACGCAACACT+TGG	-	chr4.4:54153266-54153285	MS.gene87218:CDS	55.0%
	CTAGCGGCAAACCAACCCAT+CGG	+	chr4.4:54153565-54153584	None:intergenic	55.0%
	CTGAAAGCTGCAGCTCAGGA+AGG	-	chr4.4:54152355-54152374	MS.gene87218:CDS	55.0%
	GGCACTAAATAGTGAGCCGC+AGG	-	chr4.4:54152918-54152937	MS.gene87218:intron	55.0%
	CAATCCGGATTGAGTCCCCC+TGG	-	chr4.4:54153325-54153344	MS.gene87218:CDS	60.0%
	GCAGCTGAAAGCTGCAGCTC+AGG	-	chr4.4:54152351-54152370	MS.gene87218:CDS	60.0%
	TCCACCAGGGGGACTCAATC+CGG	+	chr4.4:54153332-54153351	None:intergenic	60.0%
	GCACACGGGCACAAAGGCTC+AGG	-	chr4.4:54153182-54153201	MS.gene87218:CDS	65.0%
	TCCGGATTGAGTCCCCCTGG+TGG	-	chr4.4:54153328-54153347	MS.gene87218:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	54152331	54153584	54152331	ID=MS.gene87218
chr4.4	mRNA	54152331	54153584	54152331	ID=MS.gene87218.t1;Parent=MS.gene87218
chr4.4	exon	54152976	54153584	54152976	ID=MS.gene87218.t1.exon1;Parent=MS.gene87218.t1
chr4.4	CDS	54152976	54153584	54152976	ID=cds.MS.gene87218.t1;Parent=MS.gene87218.t1
chr4.4	exon	54152469	54152881	54152469	ID=MS.gene87218.t1.exon2;Parent=MS.gene87218.t1
chr4.4	CDS	54152469	54152881	54152469	ID=cds.MS.gene87218.t1;Parent=MS.gene87218.t1
chr4.4	exon	54152331	54152403	54152331	ID=MS.gene87218.t1.exon3;Parent=MS.gene87218.t1
chr4.4	CDS	54152331	54152403	54152331	ID=cds.MS.gene87218.t1;Parent=MS.gene87218.t1

Gene Sequence

>MS.gene87218

ATGAACACAAATATTTGTGAGCAGCTGAAAGCTGCAGCTCAGGAAGGTGATATAGATCTCCTCTATGCAGTAATCGGAGATGATCCATTCATTCTGGAGCATATAAATTTGATACCATTTGTTGAAACTCCGTTACATATTGCTGCATCTATGGGTCATATCTCGTTTACCATTGAAATTATGAATTTAAAACCTTCATTTGCTTGGAAGCTAAATCCGCAGGGATTCAGTCCCATTCATCTCGCTATGCAAAACGACCAAAAATATATAGTGTCTTTCTTTGTGCACAACAATAAAGACCTTGTTAGAGTTAAAGGAAGAGAGGGCTTAACTCCTCTTCATTTTGCAAGTCAAAATGGCGAGGTTGAATTTTTAGCGGAATTTCTCTTTCTTTGTCCGGATTCCCTTGAATACTTGACTGTGAGGTGCGAGACTGCACTGCATATTGCTGTTAAGAATCAACAATATGAGGCCCTTCAAATACTTGTTAGCTGGCTCAAACGAAATAATCGAAGAGGTGCTCAAAAGTTGGAAAATGGAATATTAAACCAGAGGGATAAAGCAGGCAACACCATTTTGCATATTTCGGCACTAAATAGTGAGCCGCAGGTAATCTTATTCAATAACTAAAATCACATGTCTCCTTTATATCTTTCTTGATTTCTTGTATTGTTATTTGTTAATTAATTTCTTGGTAGCACAGGCACTTCTATTGTTGGTAAGTACTGGTATAAATTTGGACGCAAAGAATGAGGAGAACAAAACCGCATTAGACATAGCAAGCACTCCAGAGATCAAGAGTATATTATTAAGTGTTGGAGCAAAACCGAGCTTAGAAGTCACTGATTCTCGCACACGGGCACAAAGGCTCAGGTCAAAGACCTCAAAAAGAACTATTATGTCAACTTATGTAAATCGCATTAGAAGTAATATAACAGAGGAGCAACGCAACACTTGGTTGATAGTTTCAACTCTTGTTGCAACTGCGATCTATCAATCCGGATTGAGTCCCCCTGGTGGAATTTATCAGGTTAGTGCCAGTGATGACAATGGCGTGAACAACACTTCCGCCAATTCTACTATTTCTACCCTAGAAAATGCTGGGAAATCAGTTTTGTCAGGATATGAATTCTTTTTGTTTTTATTTATAAATATGTATTCCTTTTTTGTATCAATTTTAGCGATATATGTTATGATACCAGTTGGAATACTAGGCTCTCTAGTTGGTTCTCCGATGGGTTGGTTTGCCGCTAG

Protein sequence

>MS.gene87218.t1

MNTNICEQLKAAAQEGDIDLLYAVIGDDPFILEHINLIPFVETPLHIAASMGHISFTIEIMNLKPSFAWKLNPQGFSPIHLAMQNDQKYIVSFFVHNNKDLVRVKGREGLTPLHFASQNGEVEFLAEFLFLCPDSLEYLTVRCETALHIAVKNQQYEALQILVSWLKRNNRRGAQKLENGILNQRDKAGNTILHISALNSEPQALLLLVSTGINLDAKNEENKTALDIASTPEIKSILLSVGAKPSLEVTDSRTRAQRLRSKTSKRTIMSTYVNRIRSNITEEQRNTWLIVSTLVATAIYQSGLSPPGGIYQVSASDDNGVNNTSANSTISTLENAGKSVFDICYDTSWNTRLSSWFSDGLVCR