AlfalfaGEDB | MS.gene056455

M. sativa cultivar XinJiangDaYe / MS.gene056455

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF12796.10 PF13857.9 PF13962.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene056455.t1	XP_013446189.1	88.4	465	52	1	1	463	1	465	2.80E-179	638.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene056455.t1	Q8GYH5	37.7	393	211	5	12	393	10	379	3.1e-17	91.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene056455.t1	A0A072TSN3	88.4	465	52	1	1	463	1	465	2.0e-179	638.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene056455	MS.gene51278	PPI
MS.gene056455	MS.gene69966	PPI
MS.gene056455	MS.gene87218	PPI
MS.gene056455	MS.gene039682	PPI
MS.gene010912	MS.gene056455	PPI
MS.gene056455	MS.gene025229	PPI
MS.gene056455	MS.gene30433	PPI
MS.gene056455	MS.gene066501	PPI
MS.gene056455	MS.gene043742	PPI
MS.gene056455	MS.gene027441	PPI
MS.gene056455	MS.gene21734	PPI
MS.gene056455	MS.gene30177	PPI
MS.gene056455	MS.gene70008	PPI
MS.gene056455	MS.gene008833	PPI
MS.gene056455	MS.gene67157	PPI
MS.gene056455	MS.gene23811	PPI
MS.gene056455	MS.gene25244	PPI
MS.gene056455	MS.gene69965	PPI
MS.gene056455	MS.gene82740	PPI
MS.gene056455	MS.gene051003	PPI
MS.gene056455	MS.gene48655	PPI
MS.gene056455	MS.gene09785	PPI
MS.gene056455	MS.gene64441	PPI
MS.gene056455	MS.gene066046	PPI
MS.gene056455	MS.gene007486	PPI
MS.gene056455	MS.gene78281	PPI
MS.gene056455	MS.gene035805	PPI
MS.gene056455	MS.gene23810	PPI
MS.gene056455	MS.gene08470	PPI
MS.gene056455	MS.gene027073	PPI
MS.gene056455	MS.gene34712	PPI
MS.gene056455	MS.gene65463	PPI
MS.gene056455	MS.gene007485	PPI
MS.gene039682	MS.gene056455	PPI
MS.gene056455	MS.gene007487	PPI
MS.gene056455	MS.gene037184	PPI
MS.gene056455	MS.gene23196	PPI
MS.gene056455	MS.gene067705	PPI
MS.gene47479	MS.gene056455	PPI
MS.gene056455	MS.gene71204	PPI
MS.gene056455	MS.gene41291	PPI
MS.gene056455	MS.gene027075	PPI
MS.gene056455	MS.gene061932	PPI
MS.gene056455	MS.gene050457	PPI
MS.gene056455	MS.gene99973	PPI
MS.gene056455	MS.gene30174	PPI
MS.gene056455	MS.gene26021	PPI
MS.gene056455	MS.gene050456	PPI
MS.gene056455	MS.gene064542	PPI
MS.gene056455	MS.gene91775	PPI
MS.gene056455	MS.gene25515	PPI
MS.gene056455	MS.gene34651	PPI
MS.gene056455	MS.gene89986	PPI
MS.gene056455	MS.gene22482	PPI
MS.gene056455	MS.gene050270	PPI
MS.gene056455	MS.gene052956	PPI
MS.gene056455	MS.gene60920	PPI
MS.gene056455	MS.gene058128	PPI
MS.gene056455	MS.gene34009	PPI
MS.gene69965	MS.gene056455	PPI
MS.gene056455	MS.gene018518	PPI
MS.gene056455	MS.gene043662	PPI
MS.gene056455	MS.gene065848	PPI
MS.gene056455	MS.gene004844	PPI
MS.gene056455	MS.gene068249	PPI
MS.gene056455	MS.gene99588	PPI
MS.gene056455	MS.gene005995	PPI
MS.gene056455	MS.gene54764	PPI
MS.gene056455	MS.gene24806	PPI
MS.gene056455	MS.gene038519	PPI
MS.gene056455	MS.gene27223	PPI
MS.gene056455	MS.gene042783	PPI
MS.gene30172	MS.gene056455	PPI
MS.gene056455	MS.gene25024	PPI
MS.gene056455	MS.gene012203	PPI
MS.gene056455	MS.gene26019	PPI
MS.gene056455	MS.gene97943	PPI
MS.gene23852	MS.gene056455	PPI
MS.gene056455	MS.gene050272	PPI
MS.gene056455	MS.gene63085	PPI
MS.gene056455	MS.gene69687	PPI
MS.gene056455	MS.gene26532	PPI
MS.gene056455	MS.gene30176	PPI
MS.gene056455	MS.gene51258	PPI
MS.gene056455	MS.gene32723	PPI
MS.gene056455	MS.gene010910	PPI
MS.gene056455	MS.gene053363	PPI
MS.gene056455	MS.gene066467	PPI
MS.gene010910	MS.gene056455	PPI
MS.gene056455	MS.gene024753	PPI
MS.gene063222	MS.gene056455	PPI
MS.gene056455	MS.gene32726	PPI
MS.gene056455	MS.gene71122	PPI
MS.gene056455	MS.gene24922	PPI
MS.gene056455	MS.gene73119	PPI
MS.gene056455	MS.gene92243	PPI
MS.gene69687	MS.gene056455	PPI
MS.gene056455	MS.gene85534	PPI
MS.gene056455	MS.gene037950	PPI
MS.gene056455	MS.gene027074	PPI
MS.gene056455	MS.gene51262	PPI
MS.gene056455	MS.gene23881	PPI
MS.gene056455	MS.gene26672	PPI
MS.gene056455	MS.gene014931	PPI
MS.gene056455	MS.gene062662	PPI
MS.gene056455	MS.gene21888	PPI
MS.gene056455	MS.gene97379	PPI
MS.gene056455	MS.gene066500	PPI
MS.gene056455	MS.gene23852	PPI
MS.gene78281	MS.gene056455	PPI
MS.gene056455	MS.gene008767	PPI
MS.gene056455	MS.gene39623	PPI
MS.gene056455	MS.gene36842	PPI
MS.gene056455	MS.gene066464	PPI
MS.gene056455	MS.gene07706	PPI
MS.gene056455	MS.gene071113	PPI
MS.gene056455	MS.gene90400	PPI
MS.gene056455	MS.gene022109	PPI
MS.gene027071	MS.gene056455	PPI
MS.gene056455	MS.gene041770	PPI
MS.gene056455	MS.gene81055	PPI
MS.gene056455	MS.gene65775	PPI
MS.gene056455	MS.gene99554	PPI
MS.gene056455	MS.gene80163	PPI
MS.gene056455	MS.gene02469	PPI
MS.gene056455	MS.gene33377	PPI
MS.gene056455	MS.gene51335	PPI
MS.gene056455	MS.gene045937	PPI
MS.gene056455	MS.gene027072	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene056455.t1	MTR_8g069880	92.688	465	32	1	1	463	1	465	0.0	894
MS.gene056455.t1	MTR_8g072510	57.802	455	169	6	3	452	6	442	8.35e-167	478
MS.gene056455.t1	MTR_8g039620	56.381	431	181	5	3	428	10	438	1.68e-152	441
MS.gene056455.t1	MTR_8g062410	55.687	422	178	3	3	422	6	420	3.38e-151	438
MS.gene056455.t1	MTR_8g467780	54.586	447	187	5	7	441	11	453	3.61e-151	438
MS.gene056455.t1	MTR_3g016210	57.457	409	158	4	3	395	6	414	4.82e-150	435
MS.gene056455.t1	MTR_7g021090	58.824	391	149	8	3	388	5	388	1.90e-142	414
MS.gene056455.t1	MTR_8g040930	57.029	377	152	3	50	416	2	378	1.09e-140	408
MS.gene056455.t1	MTR_8g041240	57.029	377	152	3	50	416	2	378	1.09e-140	408
MS.gene056455.t1	MTR_8g068990	59.030	371	135	4	10	377	15	371	1.88e-140	410
MS.gene056455.t1	MTR_8g073260	55.097	412	175	6	1	405	4	412	7.63e-140	409
MS.gene056455.t1	MTR_8g066670	54.920	437	192	5	2	436	1070	1503	1.68e-135	425
MS.gene056455.t1	MTR_1g059930	61.429	350	132	3	7	354	12	360	6.96e-133	390
MS.gene056455.t1	MTR_7g451420	65.806	310	104	2	2	310	4	312	5.39e-132	384
MS.gene056455.t1	MTR_3g105650	40.465	430	208	8	5	423	3	395	4.46e-98	301
MS.gene056455.t1	MTR_8g073275	44.928	414	149	9	50	395	2	404	7.69e-97	299
MS.gene056455.t1	MTR_1g069110	26.282	468	290	12	10	452	21	458	1.04e-36	140
MS.gene056455.t1	MTR_7g099580	25.333	300	173	6	16	310	21	274	2.36e-23	100
MS.gene056455.t1	MTR_2g101320	28.794	257	153	7	32	269	131	376	4.27e-22	99.8
MS.gene056455.t1	MTR_2g101310	29.843	191	122	3	38	227	137	316	1.94e-18	88.6
MS.gene056455.t1	MTR_2g101310	29.843	191	122	3	38	227	137	316	6.58e-18	86.7
MS.gene056455.t1	MTR_7g099570	27.237	257	161	6	7	247	9	255	2.98e-17	84.7
MS.gene056455.t1	MTR_1g115575	23.496	349	203	10	42	363	169	480	3.77e-17	84.3
MS.gene056455.t1	MTR_7g117620	24.759	311	190	6	26	306	97	393	3.89e-17	84.3
MS.gene056455.t1	MTR_1g032100	22.308	390	234	10	6	363	59	411	3.01e-13	72.0
MS.gene056455.t1	MTR_7g100430	26.168	321	177	12	40	307	124	437	7.45e-13	70.9
MS.gene056455.t1	MTR_4g062100	23.932	351	215	11	7	306	35	384	9.94e-13	70.5
MS.gene056455.t1	MTR_7g100390	22.667	300	192	5	43	318	88	371	3.02e-11	65.9
MS.gene056455.t1	MTR_2g096190	22.145	289	187	6	43	306	105	380	9.85e-11	64.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene056455.t1	AT4G10720	37.441	422	228	9	10	411	8	413	1.58e-82	262
MS.gene056455.t1	AT5G15500	35.506	445	250	10	7	436	6	428	3.33e-78	251
MS.gene056455.t1	AT4G10720	36.124	418	206	8	10	411	8	380	1.09e-76	246
MS.gene056455.t1	AT1G14500	33.827	405	225	6	7	394	5	383	1.38e-74	241
MS.gene056455.t1	AT1G14480	34.676	447	251	8	7	439	5	424	2.10e-74	241
MS.gene056455.t1	AT5G54620	37.047	386	212	4	12	389	10	372	2.97e-74	240
MS.gene056455.t1	AT5G54610	31.733	375	226	4	12	379	10	361	1.54e-60	204
MS.gene056455.t1	AT1G14480	31.320	447	223	10	7	439	5	381	3.39e-53	184
MS.gene056455.t1	AT5G15500	31.686	344	198	10	108	436	1	322	2.24e-42	154
MS.gene056455.t1	AT5G51160	27.907	387	218	13	61	410	35	397	1.16e-31	126
MS.gene056455.t1	AT5G54700	25.269	372	203	13	93	446	17	331	9.76e-18	84.7
MS.gene056455.t1	AT4G03500	28.505	214	141	3	94	307	301	502	1.32e-17	85.9
MS.gene056455.t1	AT4G03500	28.505	214	141	3	94	307	278	479	1.46e-17	85.9
MS.gene056455.t1	AT4G03500	28.505	214	141	3	94	307	278	479	1.46e-17	85.9
MS.gene056455.t1	AT4G03460	29.870	231	124	7	90	307	249	454	8.85e-17	83.6
MS.gene056455.t1	AT4G03460	29.870	231	124	7	90	307	281	486	9.72e-17	83.2
MS.gene056455.t1	AT3G09550	23.575	386	220	12	10	363	150	492	1.12e-16	83.2
MS.gene056455.t1	AT3G09550	23.575	386	220	12	10	363	150	492	1.12e-16	83.2
MS.gene056455.t1	AT3G09550	23.575	386	220	12	10	363	157	499	1.27e-16	82.8
MS.gene056455.t1	AT1G10340	23.500	400	234	12	77	430	175	548	1.32e-16	82.8
MS.gene056455.t1	AT1G10340	23.500	400	234	12	77	430	171	544	1.38e-16	82.8
MS.gene056455.t1	AT5G54700	24.348	345	174	9	22	364	20	279	1.39e-16	82.4
MS.gene056455.t1	AT2G24600	23.515	404	229	14	77	430	175	548	5.94e-16	80.9
MS.gene056455.t1	AT2G24600	23.515	404	229	14	77	430	175	548	5.94e-16	80.9
MS.gene056455.t1	AT1G34050	23.426	397	244	11	63	430	174	539	1.07e-15	80.1
MS.gene056455.t1	AT5G54710	25.938	320	158	11	21	310	181	451	1.17e-15	79.7
MS.gene056455.t1	AT5G54710	25.938	320	158	11	21	310	226	496	1.24e-15	79.7
MS.gene056455.t1	AT2G24600	26.493	268	153	7	77	310	175	432	1.35e-15	79.7
MS.gene056455.t1	AT2G24600	26.493	268	153	7	77	310	175	432	1.35e-15	79.7
MS.gene056455.t1	AT1G03670	28.634	227	112	6	99	307	279	473	1.80e-15	79.3
MS.gene056455.t1	AT3G12360	25.788	349	177	13	22	307	114	443	1.94e-15	79.3
MS.gene056455.t1	AT3G12360	25.788	349	177	13	22	307	102	431	2.37e-15	79.0
MS.gene056455.t1	AT2G31820	25.846	325	188	13	19	306	193	501	9.54e-15	77.0
MS.gene056455.t1	AT1G05640	25.260	289	178	8	42	306	191	465	6.51e-14	74.3
MS.gene056455.t1	AT4G03490	23.188	345	164	10	41	307	146	467	1.47e-11	67.0
MS.gene056455.t1	AT4G03490	23.188	345	164	10	41	307	136	457	1.53e-11	67.0
MS.gene056455.t1	AT1G07710	23.438	320	209	5	10	306	73	379	1.61e-11	66.6
MS.gene056455.t1	AT1G07710	23.438	320	209	5	10	306	73	379	1.61e-11	66.6
MS.gene056455.t1	AT4G03490	23.188	345	164	10	41	307	146	467	1.78e-11	67.0
MS.gene056455.t1	AT4G03480	23.929	280	174	8	58	322	375	630	2.32e-11	66.6
MS.gene056455.t1	AT4G03480	23.509	285	181	8	58	327	372	634	2.42e-11	66.6

Find 86 sgRNAs with CRISPR-Local

Find 113 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTCAGAAGTTGATTTCTTTC+GGG	0.234667	8.3:-32799487	MS.gene056455:CDS
AAGGACCTTGTCAAAGTTAA+AGG	0.247391	8.3:-32800358	MS.gene056455:CDS
AAAGGTCAATCTCTCCAATT+TGG	0.255210	8.3:+32800302	None:intergenic
TGTCAGAAGTTGATTTCTTT+CGG	0.264542	8.3:-32799488	MS.gene056455:CDS
CTTCATTATGCAAGCCAAAT+TGG	0.271372	8.3:-32800316	MS.gene056455:CDS
TCTATCTCCCTCAAAATAGA+TGG	0.305208	8.3:+32800568	None:intergenic
GGTAAAGGACCTAGAAAATA+TGG	0.309999	8.3:-32799145	MS.gene056455:CDS
ATCACTGTCTTGATTATTGC+GGG	0.317234	8.3:-32799261	MS.gene056455:CDS
TGTTGCCTGCTACATCCTTC+TGG	0.332367	8.3:+32800104	None:intergenic
TGCATATCGCCGCATTTAAC+GGG	0.350419	8.3:-32800519	MS.gene056455:CDS
TAGGGTAGAAATAGTAGAAT+TGG	0.356230	8.3:+32799533	None:intergenic
CCATGAGAAAATAAGCTAAA+AGG	0.378679	8.3:+32800284	None:intergenic
TACTGCAATTGACATCAAAA+AGG	0.383470	8.3:+32799443	None:intergenic
ATTTCTTTCGGGTTTCATAT+TGG	0.387412	8.3:-32799476	MS.gene056455:CDS
TTTAACTCCAACTGGAAAAG+AGG	0.387649	8.3:-32799412	MS.gene056455:CDS
TTGCATATCGCCGCATTTAA+CGG	0.389697	8.3:-32800520	MS.gene056455:CDS
AATAATCAAGACAGTGATAC+TGG	0.397084	8.3:+32799266	None:intergenic
CCTAGGAAATGCTGGAAAAT+CGG	0.405154	8.3:-32799514	MS.gene056455:CDS
GTCAAGTATTCAATAGATTC+AGG	0.406411	8.3:+32800256	None:intergenic
TTTGAAGACAAAGAACTTTG+AGG	0.425247	8.3:-32799862	MS.gene056455:CDS
ATATTACCCGCTTCAGCTGC+AGG	0.439778	8.3:+32800622	None:intergenic
TTTGCATGGCAAGGTGGATA+GGG	0.440017	8.3:+32800422	None:intergenic
AACAACCATGGCTGTGGTAA+AGG	0.443309	8.3:-32799160	MS.gene056455:CDS
AGTATATCAAGATTGGTCAT+TGG	0.445141	8.3:+32800649	None:intergenic
GGGACTTAGTACTGATTGAT+AGG	0.446579	8.3:+32799611	None:intergenic
AACTAAGAAGATCAGTGTTA+AGG	0.448931	8.3:-32799203	MS.gene056455:CDS
CATAGAATATAGATAACTAA+TGG	0.452093	8.3:+32799356	None:intergenic
TTCATAACCTCGATGGCAAA+CGG	0.453946	8.3:+32800490	None:intergenic
GGTAGAAATAGTAGAATTGG+AGG	0.454556	8.3:+32799536	None:intergenic
TTTGAACTTAGTTTATATGC+AGG	0.469966	8.3:+32799744	None:intergenic
TGAATCTATTGAATACTTGA+CGG	0.472640	8.3:-32800254	MS.gene056455:CDS
AGGACCTTGTCAAAGTTAAA+GGG	0.480217	8.3:-32800357	MS.gene056455:CDS
ATTTCTACCCTAGGAAATGC+TGG	0.480779	8.3:-32799522	MS.gene056455:CDS
TGACAGTTTATCAATAATAG+TGG	0.484355	8.3:+32799719	None:intergenic
CCCTAAAAGTACTAGTTGGC+TGG	0.491416	8.3:-32800180	MS.gene056455:CDS
TTTAGTTTGAAAGCAAATGA+AGG	0.492735	8.3:+32800460	None:intergenic
CTTAGACCTGCAGCTGAAGC+GGG	0.492988	8.3:-32800628	MS.gene056455:CDS
TCATAACCTCGATGGCAAAC+GGG	0.493794	8.3:+32800491	None:intergenic
CTAATGGCAAACCAAGTCAC+TGG	0.500982	8.3:+32799372	None:intergenic
AAAAGTACTAGTTGGCTGGC+TGG	0.501290	8.3:-32800176	MS.gene056455:CDS
TTAACTCCAACTGGAAAAGA+GGG	0.507555	8.3:-32799411	MS.gene056455:CDS
AATTCTACTATTTCTACCCT+AGG	0.517193	8.3:-32799531	MS.gene056455:CDS
CAATGTACATCCCAGTGACT+TGG	0.520189	8.3:-32799383	MS.gene056455:CDS
CCTTCCCTTTAACTTTGACA+AGG	0.523374	8.3:+32800353	None:intergenic
TAGGTCCTTTACCACAGCCA+TGG	0.526478	8.3:+32799155	None:intergenic
AGGTCTAAGTATATCAAGAT+TGG	0.530471	8.3:+32800642	None:intergenic
CAGGACAGGTGCTATAGAGC+TGG	0.534380	8.3:-32800143	MS.gene056455:CDS
CTATCTCCCTCAAAATAGAT+GGG	0.536487	8.3:+32800569	None:intergenic
CAAGTCACTGGGATGTACAT+TGG	0.540352	8.3:+32799384	None:intergenic
ACACGGCATTGAAATGTGTA+GGG	0.543366	8.3:+32799322	None:intergenic
GAAGATCAGTGTTAAGGCAT+CGG	0.551823	8.3:-32799197	MS.gene056455:CDS
GTCAAAGTTAAAGGGAAGGA+AGG	0.554942	8.3:-32800349	MS.gene056455:CDS
AGCATTAGACATAGCAACTA+CGG	0.556154	8.3:-32799832	MS.gene056455:CDS
TATCACTGTCTTGATTATTG+CGG	0.560106	8.3:-32799262	MS.gene056455:CDS
TTTCAAATTCATAACCTCGA+TGG	0.561291	8.3:+32800483	None:intergenic
ACTTAGACCTGCAGCTGAAG+CGG	0.565189	8.3:-32800629	MS.gene056455:CDS
AAGAAAATATTATGCAGTGC+CGG	0.569979	8.3:-32799804	MS.gene056455:CDS
CTGAACCAGAAGGATGTAGC+AGG	0.571796	8.3:-32800109	MS.gene056455:CDS
GCTTTCAAACTAAATAAGCA+AGG	0.582906	8.3:-32800451	MS.gene056455:CDS
GATAGGTGGCAGTCGCAACA+AGG	0.584419	8.3:+32799628	None:intergenic
AGAAACAACAACCATGGCTG+TGG	0.585849	8.3:-32799166	MS.gene056455:CDS
ATTTGGCTTGCATAATGAAG+AGG	0.588329	8.3:+32800319	None:intergenic
GACGCCCTAAAAGTACTAGT+TGG	0.588930	8.3:-32800184	MS.gene056455:CDS
TTAACCTGATAAACTCCACC+AGG	0.594570	8.3:+32799588	None:intergenic
GGTACTTTAGTGTCCAGAAG+TGG	0.609035	8.3:-32799239	MS.gene056455:CDS
TTAAATGCGGCGATATGCAA+AGG	0.609641	8.3:+32800523	None:intergenic
CCTTGTCAAAGTTAAAGGGA+AGG	0.610782	8.3:-32800353	MS.gene056455:CDS
ATGCCAGGTAGTTAGACTGT+TGG	0.624876	8.3:-32799901	MS.gene056455:intron
AAATAAAATACTGAACCAGA+AGG	0.625252	8.3:-32800119	MS.gene056455:CDS
TGTGTGAGCATCGGTGACTT+CGG	0.631735	8.3:+32799767	None:intergenic
GCATCTCCCGTTTGCCATCG+AGG	0.640153	8.3:-32800497	MS.gene056455:CDS
ATATGCAGGTGTGTGAGCAT+CGG	0.640634	8.3:+32799758	None:intergenic
AACACGGCATTGAAATGTGT+AGG	0.642219	8.3:+32799321	None:intergenic
TAATGGCAAACCAAGTCACT+GGG	0.645311	8.3:+32799373	None:intergenic
CTATTGAATACTTGACGGTG+AGG	0.647961	8.3:-32800249	MS.gene056455:CDS
CGGGAGATGCCCGTTAAATG+CGG	0.651361	8.3:+32800510	None:intergenic
ATAGGTGGCAGTCGCAACAA+GGG	0.651423	8.3:+32799629	None:intergenic
TTCAAGAAGTGATCAACATG+AGG	0.657499	8.3:-32800062	MS.gene056455:intron
GCAAAAGAAAGAAAAGACAA+TGG	0.660962	8.3:-32800404	MS.gene056455:CDS
CACGGCATTGAAATGTGTAG+GGG	0.667888	8.3:+32799323	None:intergenic
ACTTAGTACTGATTGATAGG+TGG	0.680031	8.3:+32799614	None:intergenic
TATAGCAGAAACAACAACCA+TGG	0.681000	8.3:-32799172	MS.gene056455:CDS
TAACCTGATAAACTCCACCA+GGG	0.698278	8.3:+32799589	None:intergenic
AACCTGATAAACTCCACCAG+GGG	0.700058	8.3:+32799590	None:intergenic
TCACTGTCTTGATTATTGCG+GGG	0.701834	8.3:-32799260	MS.gene056455:CDS
ACCTGATAAACTCCACCAGG+GGG	0.706222	8.3:+32799591	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATTGTTCATTGATTATTAAT+CGG	-	chr8.3:32799852-32799871	MS.gene056455:CDS	15.0%
!!	CATAGAATATAGATAACTAA+TGG	+	chr8.3:32800424-32800443	None:intergenic	20.0%
!	AAAAAACATCTCAACAAACA+CGG	+	chr8.3:32800475-32800494	None:intergenic	25.0%
!	AAATAAAATACTGAACCAGA+AGG	-	chr8.3:32799658-32799677	MS.gene056455:CDS	25.0%
!	TGAATCTATTGAATACTTGA+CGG	-	chr8.3:32799523-32799542	MS.gene056455:CDS	25.0%
!	TGACAGTTTATCAATAATAG+TGG	+	chr8.3:32800061-32800080	None:intergenic	25.0%
!	TTTGAACTTAGTTTATATGC+AGG	+	chr8.3:32800036-32800055	None:intergenic	25.0%
!!!	ATACTTATTTTAACTCCAAC+TGG	-	chr8.3:32800357-32800376	MS.gene056455:CDS	25.0%
!!!	CTTTTTCAATTACAGTATAG+AGG	+	chr8.3:32799187-32799206	None:intergenic	25.0%
!!!	TGAATACTTTTAAAGCAGAT+TGG	-	chr8.3:32799767-32799786	MS.gene056455:CDS	25.0%
!!!	TTTAGTTTGAAAGCAAATGA+AGG	+	chr8.3:32799320-32799339	None:intergenic	25.0%
	AACTAAGAAGATCAGTGTTA+AGG	-	chr8.3:32800574-32800593	MS.gene056455:CDS	30.0%
	AAGAAAATATTATGCAGTGC+CGG	-	chr8.3:32799973-32799992	MS.gene056455:intron	30.0%
	AATAATCAAGACAGTGATAC+TGG	+	chr8.3:32800514-32800533	None:intergenic	30.0%
	AGGTCTAAGTATATCAAGAT+TGG	+	chr8.3:32799138-32799157	None:intergenic	30.0%
	CCATGAGAAAATAAGCTAAA+AGG	+	chr8.3:32799496-32799515	None:intergenic	30.0%
	GCAAAAGAAAGAAAAGACAA+TGG	-	chr8.3:32799373-32799392	MS.gene056455:CDS	30.0%
	GCTTTCAAACTAAATAAGCA+AGG	-	chr8.3:32799326-32799345	MS.gene056455:CDS	30.0%
	GTCAAGTATTCAATAGATTC+AGG	+	chr8.3:32799524-32799543	None:intergenic	30.0%
	TACTGCAATTGACATCAAAA+AGG	+	chr8.3:32800337-32800356	None:intergenic	30.0%
	TAGGGTAGAAATAGTAGAAT+TGG	+	chr8.3:32800247-32800266	None:intergenic	30.0%
	TATCACTGTCTTGATTATTG+CGG	-	chr8.3:32800515-32800534	MS.gene056455:CDS	30.0%
	TTTCAAATTCATAACCTCGA+TGG	+	chr8.3:32799297-32799316	None:intergenic	30.0%
	TTTGAAGACAAAGAACTTTG+AGG	-	chr8.3:32799915-32799934	MS.gene056455:intron	30.0%
!	AAAAAGTCCCATCTATTTTG+AGG	-	chr8.3:32799201-32799220	MS.gene056455:CDS	30.0%
!	TCTGTGAATCCATATTTTCT+AGG	+	chr8.3:32800644-32800663	None:intergenic	30.0%
!	TGTCAGAAGTTGATTTCTTT+CGG	-	chr8.3:32800289-32800308	MS.gene056455:CDS	30.0%
!	TGTCTTTTCTTTCTTTTGCA+TGG	+	chr8.3:32799372-32799391	None:intergenic	30.0%
!!	AAAAGTCCCATCTATTTTGA+GGG	-	chr8.3:32799202-32799221	MS.gene056455:CDS	30.0%
!!	AATTCTACTATTTCTACCCT+AGG	-	chr8.3:32800246-32800265	MS.gene056455:CDS	30.0%
!!	ATTTCTTTCGGGTTTCATAT+TGG	-	chr8.3:32800301-32800320	MS.gene056455:CDS	30.0%
!!!	CCTTTTAGCTTATTTTCTCA+TGG	-	chr8.3:32799493-32799512	MS.gene056455:CDS	30.0%
	AAAGGTCAATCTCTCCAATT+TGG	+	chr8.3:32799478-32799497	None:intergenic	35.0%
	AAGGACCTTGTCAAAGTTAA+AGG	-	chr8.3:32799419-32799438	MS.gene056455:CDS	35.0%
	AGCATTAGACATAGCAACTA+CGG	-	chr8.3:32799945-32799964	MS.gene056455:intron	35.0%
	AGGACCTTGTCAAAGTTAAA+GGG	-	chr8.3:32799420-32799439	MS.gene056455:CDS	35.0%
	ATCACTGTCTTGATTATTGC+GGG	-	chr8.3:32800516-32800535	MS.gene056455:CDS	35.0%
	CTATCTCCCTCAAAATAGAT+GGG	+	chr8.3:32799211-32799230	None:intergenic	35.0%
	CTTCATTATGCAAGCCAAAT+TGG	-	chr8.3:32799461-32799480	MS.gene056455:CDS	35.0%
	GGTAAAGGACCTAGAAAATA+TGG	-	chr8.3:32800632-32800651	MS.gene056455:CDS	35.0%
	GGTAGAAATAGTAGAATTGG+AGG	+	chr8.3:32800244-32800263	None:intergenic	35.0%
	TATAGCAGAAACAACAACCA+TGG	-	chr8.3:32800605-32800624	MS.gene056455:CDS	35.0%
	TCTATCTCCCTCAAAATAGA+TGG	+	chr8.3:32799212-32799231	None:intergenic	35.0%
	TGATTATTAATCGGTATGCC+AGG	-	chr8.3:32799861-32799880	MS.gene056455:CDS	35.0%
	TTAACTCCAACTGGAAAAGA+GGG	-	chr8.3:32800366-32800385	MS.gene056455:CDS	35.0%
	TTCAAGAAGTGATCAACATG+AGG	-	chr8.3:32799715-32799734	MS.gene056455:CDS	35.0%
	TTTAACTCCAACTGGAAAAG+AGG	-	chr8.3:32800365-32800384	MS.gene056455:CDS	35.0%
!	GTCAGAAGTTGATTTCTTTC+GGG	-	chr8.3:32800290-32800309	MS.gene056455:CDS	35.0%
!	TTTCTTTCTTTTGCATGGCA+AGG	+	chr8.3:32799367-32799386	None:intergenic	35.0%
!!	ACTTAGTACTGATTGATAGG+TGG	+	chr8.3:32800166-32800185	None:intergenic	35.0%
!!	ATTTGGCTTGCATAATGAAG+AGG	+	chr8.3:32799461-32799480	None:intergenic	35.0%
!!	GTGTTTTGTTGACATGAACA+AGG	-	chr8.3:32799400-32799419	MS.gene056455:CDS	35.0%
!!	TCTTAGCTTTTATCCACTTC+TGG	+	chr8.3:32800554-32800573	None:intergenic	35.0%
	ATTTCTACCCTAGGAAATGC+TGG	-	chr8.3:32800255-32800274	MS.gene056455:CDS	40.0%
	CCTAGGAAATGCTGGAAAAT+CGG	-	chr8.3:32800263-32800282	MS.gene056455:CDS	40.0%
	CCTTGTCAAAGTTAAAGGGA+AGG	-	chr8.3:32799424-32799443	MS.gene056455:CDS	40.0%
	CTATTGAATACTTGACGGTG+AGG	-	chr8.3:32799528-32799547	MS.gene056455:CDS	40.0%
	CTGGAAAAAGATTACAGGAC+AGG	-	chr8.3:32799620-32799639	MS.gene056455:CDS	40.0%
	GAAGATCAGTGTTAAGGCAT+CGG	-	chr8.3:32800580-32800599	MS.gene056455:CDS	40.0%
	GTCAAAGTTAAAGGGAAGGA+AGG	-	chr8.3:32799428-32799447	MS.gene056455:CDS	40.0%
	TAACCTGATAAACTCCACCA+GGG	+	chr8.3:32800191-32800210	None:intergenic	40.0%
	TAATGGCAAACCAAGTCACT+GGG	+	chr8.3:32800407-32800426	None:intergenic	40.0%
	TCACTGTCTTGATTATTGCG+GGG	-	chr8.3:32800517-32800536	MS.gene056455:CDS	40.0%
	TTAAATGCGGCGATATGCAA+AGG	+	chr8.3:32799257-32799276	None:intergenic	40.0%
	TTAACCTGATAAACTCCACC+AGG	+	chr8.3:32800192-32800211	None:intergenic	40.0%
	TTACCAACAGTCTAACTACC+TGG	+	chr8.3:32799882-32799901	None:intergenic	40.0%
	TTCATAACCTCGATGGCAAA+CGG	+	chr8.3:32799290-32799309	None:intergenic	40.0%
	TTGCATATCGCCGCATTTAA+CGG	-	chr8.3:32799257-32799276	MS.gene056455:CDS	40.0%
!	AACACGGCATTGAAATGTGT+AGG	+	chr8.3:32800459-32800478	None:intergenic	40.0%
!	ACACGGCATTGAAATGTGTA+GGG	+	chr8.3:32800458-32800477	None:intergenic	40.0%
!	CCAGCCAACTAGTACTTTTA+GGG	+	chr8.3:32799600-32799619	None:intergenic	40.0%
!	CCTTCCCTTTAACTTTGACA+AGG	+	chr8.3:32799427-32799446	None:intergenic	40.0%
!	CGATTTTCCAGCATTTCCTA+GGG	+	chr8.3:32800265-32800284	None:intergenic	40.0%
!!	GGGACTTAGTACTGATTGAT+AGG	+	chr8.3:32800169-32800188	None:intergenic	40.0%
	AACAACCATGGCTGTGGTAA+AGG	-	chr8.3:32800617-32800636	MS.gene056455:CDS	45.0%
	AACCTGATAAACTCCACCAG+GGG	+	chr8.3:32800190-32800209	None:intergenic	45.0%
	AGAAACAACAACCATGGCTG+TGG	-	chr8.3:32800611-32800630	MS.gene056455:CDS	45.0%
	ATATGCAGGTGTGTGAGCAT+CGG	+	chr8.3:32800022-32800041	None:intergenic	45.0%
	ATGCCAGGTAGTTAGACTGT+TGG	-	chr8.3:32799876-32799895	MS.gene056455:CDS	45.0%
	CAAGTCACTGGGATGTACAT+TGG	+	chr8.3:32800396-32800415	None:intergenic	45.0%
	CAATGTACATCCCAGTGACT+TGG	-	chr8.3:32800394-32800413	MS.gene056455:CDS	45.0%
	CTAATGGCAAACCAAGTCAC+TGG	+	chr8.3:32800408-32800427	None:intergenic	45.0%
	GCTGGCTGGAAAAAGATTAC+AGG	-	chr8.3:32799615-32799634	MS.gene056455:CDS	45.0%
	GGTACTTTAGTGTCCAGAAG+TGG	-	chr8.3:32800538-32800557	MS.gene056455:CDS	45.0%
	TCATAACCTCGATGGCAAAC+GGG	+	chr8.3:32799289-32799308	None:intergenic	45.0%
	TGCATATCGCCGCATTTAAC+GGG	-	chr8.3:32799258-32799277	MS.gene056455:CDS	45.0%
	TTTGCATGGCAAGGTGGATA+GGG	+	chr8.3:32799358-32799377	None:intergenic	45.0%
!	CACGGCATTGAAATGTGTAG+GGG	+	chr8.3:32800457-32800476	None:intergenic	45.0%
!	CCGATTTTCCAGCATTTCCT+AGG	+	chr8.3:32800266-32800285	None:intergenic	45.0%
!	CTTTCTTTTGCATGGCAAGG+TGG	+	chr8.3:32799364-32799383	None:intergenic	45.0%
!	GCCAGCCAACTAGTACTTTT+AGG	+	chr8.3:32799601-32799620	None:intergenic	45.0%
!	TTTTGCATGGCAAGGTGGAT+AGG	+	chr8.3:32799359-32799378	None:intergenic	45.0%
!!	AAAAGTACTAGTTGGCTGGC+TGG	-	chr8.3:32799601-32799620	MS.gene056455:CDS	45.0%
!!	CCCTAAAAGTACTAGTTGGC+TGG	-	chr8.3:32799597-32799616	MS.gene056455:CDS	45.0%
!!	GACGCCCTAAAAGTACTAGT+TGG	-	chr8.3:32799593-32799612	MS.gene056455:CDS	45.0%
!!!	TTCGGTGCTAGATTTTGCTC+CGG	+	chr8.3:32799995-32800014	None:intergenic	45.0%
	ACCTGATAAACTCCACCAGG+GGG	+	chr8.3:32800189-32800208	None:intergenic	50.0%
	ACTTAGACCTGCAGCTGAAG+CGG	-	chr8.3:32799148-32799167	MS.gene056455:CDS	50.0%
	ATAGGTGGCAGTCGCAACAA+GGG	+	chr8.3:32800151-32800170	None:intergenic	50.0%
	ATATTACCCGCTTCAGCTGC+AGG	+	chr8.3:32799158-32799177	None:intergenic	50.0%
	CAATCAGTACTAAGTCCCCC+TGG	-	chr8.3:32800171-32800190	MS.gene056455:CDS	50.0%
	CTGAACCAGAAGGATGTAGC+AGG	-	chr8.3:32799668-32799687	MS.gene056455:CDS	50.0%
	TAGGTCCTTTACCACAGCCA+TGG	+	chr8.3:32800625-32800644	None:intergenic	50.0%
	TGTTGCCTGCTACATCCTTC+TGG	+	chr8.3:32799676-32799695	None:intergenic	50.0%
!!	TGTGTGAGCATCGGTGACTT+CGG	+	chr8.3:32800013-32800032	None:intergenic	50.0%
	CGGGAGATGCCCGTTAAATG+CGG	+	chr8.3:32799270-32799289	None:intergenic	55.0%
	CTTAGACCTGCAGCTGAAGC+GGG	-	chr8.3:32799149-32799168	MS.gene056455:CDS	55.0%
	GATAGGTGGCAGTCGCAACA+AGG	+	chr8.3:32800152-32800171	None:intergenic	55.0%
	TCAGTACTAAGTCCCCCTGG+TGG	-	chr8.3:32800174-32800193	MS.gene056455:CDS	55.0%
	TCCCCCTGGTGGAGTTTATC+AGG	-	chr8.3:32800185-32800204	MS.gene056455:CDS	55.0%
!	CAGGACAGGTGCTATAGAGC+TGG	-	chr8.3:32799634-32799653	MS.gene056455:CDS	55.0%
!	GGACTGCCCTCTTTTCCAGT+TGG	+	chr8.3:32800375-32800394	None:intergenic	55.0%
	GCATCTCCCGTTTGCCATCG+AGG	-	chr8.3:32799280-32799299	MS.gene056455:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.3	gene	32799131	32800668	32799131	ID=MS.gene056455
chr8.3	mRNA	32799131	32800668	32799131	ID=MS.gene056455.t1;Parent=MS.gene056455
chr8.3	exon	32800063	32800668	32800063	ID=MS.gene056455.t1.exon1;Parent=MS.gene056455.t1
chr8.3	CDS	32800063	32800668	32800063	ID=cds.MS.gene056455.t1;Parent=MS.gene056455.t1
chr8.3	exon	32799131	32799916	32799131	ID=MS.gene056455.t1.exon2;Parent=MS.gene056455.t1
chr8.3	CDS	32799131	32799916	32799131	ID=cds.MS.gene056455.t1;Parent=MS.gene056455.t1

Gene Sequence

>MS.gene056455

ATGACCAATCTTGATATACTTAGACCTGCAGCTGAAGCGGGTAATATAGAAGACCTCTATACTGTAATTGAAAAAGTCCCATCTATTTTGAGGGAGATAGATTTAGAACAATTTGTTGAAACTCCTTTGCATATCGCCGCATTTAACGGGCATCTCCCGTTTGCCATCGAGGTTATGAATTTGAAACCTTCATTTGCTTTCAAACTAAATAAGCAAGGATTCAGCCCTATCCACCTTGCCATGCAAAAGAAAGAAAAGACAATGGTGTTGTGTTTTGTTGACATGAACAAGGACCTTGTCAAAGTTAAAGGGAAGGAAGGCTTGACTCCTCTTCATTATGCAAGCCAAATTGGAGAGATTGACCTTTTAGCTTATTTTCTCATGGCATGTCCTGAATCTATTGAATACTTGACGGTGAGGTGCGAGACTGCACTTCATATTGCTATTAATAATGCGCAGTTCGACGCCCTAAAAGTACTAGTTGGCTGGCTGGAAAAAGATTACAGGACAGGTGCTATAGAGCTGGAAAATAAAATACTGAACCAGAAGGATGTAGCAGGCAACACTATTTTGCACATTTCAGCTTCAAGAAGTGATCAACATGAGGTAATCGTTATTTTATTGAAAGAATTCAAATGAATACTTTTAAAGCAGATTGGTGATTACTAGTACATTCTTCTGAATATTTTAGTAGCTAACATGAATGATCTCTAACAATTGTATTGTTCATTGATTATTAATCGGTATGCCAGGTAGTTAGACTGTTGGTAAATACTAAAATAAATTTGAAGACAAAGAACTTTGAGGACAAAACAGCATTAGACATAGCAACTACGGAGAGCAAGAAAATATTATGCAGTGCCGGAGCAAAATCTAGCACCGAAGTCACCGATGCTCACACACCTGCATATAAACTAAGTTCAAAGACCACTATTATTGATAAACTGTCAATTTATATGCTTCGCATATCACGAAATATAAAAGATGAGCAACGTAACACTATGTTGATAGTCGCAGCCCTTGTTGCGACTGCCACCTATCAATCAGTACTAAGTCCCCCTGGTGGAGTTTATCAGGTTAATGCTAGTGACAAAAATTTGAACATCACTTCCTCCAATTCTACTATTTCTACCCTAGGAAATGCTGGAAAATCGGTCATGTCAGAAGTTGATTTCTTTCGGGTTTCATATTGGAATATAGTTTCCTTTTTGATGTCAATTGCAGTAATACTTATTTTAACTCCAACTGGAAAAGAGGGCAGTCCAATGTACATCCCAGTGACTTGGTTTGCCATTAGTTATCTATATTCTATGCGAGTGATATCCCCTACACATTTCAATGCCGTGTTTGTTGAGATGTTTTTTAATTTAATTACGTTTTCCAGTATCACTGTCTTGATTATTGCGGGGTACTTTAGTGTCCAGAAGTGGATAAAAGCTAAGAAACTAAGAAGATCAGTGTTAAGGCATCGGAATATAGCAGAAACAACAACCATGGCTGTGGTAAAGGACCTAGAAAATATGGATTCACAGATTTAA

Protein sequence

>MS.gene056455.t1

MTNLDILRPAAEAGNIEDLYTVIEKVPSILREIDLEQFVETPLHIAAFNGHLPFAIEVMNLKPSFAFKLNKQGFSPIHLAMQKKEKTMVLCFVDMNKDLVKVKGKEGLTPLHYASQIGEIDLLAYFLMACPESIEYLTVRCETALHIAINNAQFDALKVLVGWLEKDYRTGAIELENKILNQKDVAGNTILHISASRSDQHEVVRLLVNTKINLKTKNFEDKTALDIATTESKKILCSAGAKSSTEVTDAHTPAYKLSSKTTIIDKLSIYMLRISRNIKDEQRNTMLIVAALVATATYQSVLSPPGGVYQVNASDKNLNITSSNSTISTLGNAGKSVMSEVDFFRVSYWNIVSFLMSIAVILILTPTGKEGSPMYIPVTWFAISYLYSMRVISPTHFNAVFVEMFFNLITFSSITVLIIAGYFSVQKWIKAKKLRRSVLRHRNIAETTTMAVVKDLENMDSQI