AlfalfaGEDB | MS.gene32726

M. sativa cultivar XinJiangDaYe / MS.gene32726

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF12796.10 PF13637.9 PF13857.9 PF13962.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32726.t1	XP_003603259.1	96.7	427	14	0	1	427	1	427	2.90E-178	634.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32726.t1	Q8GYH5	47.4	378	188	6	1	370	1	375	8.3e-25	116.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32726.t1	G7J969	96.7	427	14	0	1	427	1	427	2.1e-178	634.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene010910	MS.gene32726	PPI
MS.gene025229	MS.gene32726	PPI
MS.gene32726	MS.gene71122	PPI
MS.gene067705	MS.gene32726	PPI
MS.gene063222	MS.gene32726	PPI
MS.gene25515	MS.gene32726	PPI
MS.gene07706	MS.gene32726	PPI
MS.gene027074	MS.gene32726	PPI
MS.gene32726	MS.gene60920	PPI
MS.gene32726	MS.gene92243	PPI
MS.gene69687	MS.gene32726	PPI
MS.gene87218	MS.gene32726	PPI
MS.gene25024	MS.gene32726	PPI
MS.gene23811	MS.gene32726	PPI
MS.gene51258	MS.gene32726	PPI
MS.gene23810	MS.gene32726	PPI
MS.gene51335	MS.gene32726	PPI
MS.gene024753	MS.gene32726	PPI
MS.gene78281	MS.gene32726	PPI
MS.gene005995	MS.gene32726	PPI
MS.gene037950	MS.gene32726	PPI
MS.gene70008	MS.gene32726	PPI
MS.gene068249	MS.gene32726	PPI
MS.gene051003	MS.gene32726	PPI
MS.gene64441	MS.gene32726	PPI
MS.gene24806	MS.gene32726	PPI
MS.gene004844	MS.gene32726	PPI
MS.gene30176	MS.gene32726	PPI
MS.gene32726	MS.gene65775	PPI
MS.gene32726	MS.gene80163	PPI
MS.gene32723	MS.gene32726	PPI
MS.gene007487	MS.gene32726	PPI
MS.gene007485	MS.gene32726	PPI
MS.gene018518	MS.gene32726	PPI
MS.gene037184	MS.gene32726	PPI
MS.gene066464	MS.gene32726	PPI
MS.gene32726	MS.gene51278	PPI
MS.gene26021	MS.gene32726	PPI
MS.gene32726	MS.gene87218	PPI
MS.gene51262	MS.gene32726	PPI
MS.gene050270	MS.gene32726	PPI
MS.gene02469	MS.gene32726	PPI
MS.gene008833	MS.gene32726	PPI
MS.gene24922	MS.gene32726	PPI
MS.gene027073	MS.gene32726	PPI
MS.gene064542	MS.gene32726	PPI
MS.gene47479	MS.gene32726	PPI
MS.gene32726	MS.gene23881	PPI
MS.gene010912	MS.gene32726	PPI
MS.gene050457	MS.gene32726	PPI
MS.gene008767	MS.gene32726	PPI
MS.gene34712	MS.gene32726	PPI
MS.gene39623	MS.gene32726	PPI
MS.gene065848	MS.gene32726	PPI
MS.gene027441	MS.gene32726	PPI
MS.gene36842	MS.gene32726	PPI
MS.gene99554	MS.gene32726	PPI
MS.gene066046	MS.gene32726	PPI
MS.gene99588	MS.gene32726	PPI
MS.gene027075	MS.gene32726	PPI
MS.gene41291	MS.gene32726	PPI
MS.gene27223	MS.gene32726	PPI
MS.gene038519	MS.gene32726	PPI
MS.gene050272	MS.gene32726	PPI
MS.gene81055	MS.gene32726	PPI
MS.gene32726	MS.gene027072	PPI
MS.gene052956	MS.gene32726	PPI
MS.gene91775	MS.gene32726	PPI
MS.gene22482	MS.gene32726	PPI
MS.gene043662	MS.gene32726	PPI
MS.gene34009	MS.gene32726	PPI
MS.gene039682	MS.gene32726	PPI
MS.gene043742	MS.gene32726	PPI
MS.gene061932	MS.gene32726	PPI
MS.gene48655	MS.gene32726	PPI
MS.gene85534	MS.gene32726	PPI
MS.gene32726	MS.gene067705	PPI
MS.gene26532	MS.gene32726	PPI
MS.gene042783	MS.gene32726	PPI
MS.gene066467	MS.gene32726	PPI
MS.gene21734	MS.gene32726	PPI
MS.gene33377	MS.gene32726	PPI
MS.gene23196	MS.gene32726	PPI
MS.gene32726	MS.gene99973	PPI
MS.gene71204	MS.gene32726	PPI
MS.gene007486	MS.gene32726	PPI
MS.gene80163	MS.gene32726	PPI
MS.gene041770	MS.gene32726	PPI
MS.gene32726	MS.gene008833	PPI
MS.gene71122	MS.gene32726	PPI
MS.gene26672	MS.gene32726	PPI
MS.gene97379	MS.gene32726	PPI
MS.gene99973	MS.gene32726	PPI
MS.gene050456	MS.gene32726	PPI
MS.gene058128	MS.gene32726	PPI
MS.gene65775	MS.gene32726	PPI
MS.gene32726	MS.gene48655	PPI
MS.gene066501	MS.gene32726	PPI
MS.gene25244	MS.gene32726	PPI
MS.gene014931	MS.gene32726	PPI
MS.gene08470	MS.gene32726	PPI
MS.gene63085	MS.gene32726	PPI
MS.gene056455	MS.gene32726	PPI
MS.gene89986	MS.gene32726	PPI
MS.gene26019	MS.gene32726	PPI
MS.gene30174	MS.gene32726	PPI
MS.gene23881	MS.gene32726	PPI
MS.gene062662	MS.gene32726	PPI
MS.gene73119	MS.gene32726	PPI
MS.gene035805	MS.gene32726	PPI
MS.gene30177	MS.gene32726	PPI
MS.gene69965	MS.gene32726	PPI
MS.gene65463	MS.gene32726	PPI
MS.gene066500	MS.gene32726	PPI
MS.gene21888	MS.gene32726	PPI
MS.gene82740	MS.gene32726	PPI
MS.gene09785	MS.gene32726	PPI
MS.gene54764	MS.gene32726	PPI
MS.gene30172	MS.gene32726	PPI
MS.gene045937	MS.gene32726	PPI
MS.gene51278	MS.gene32726	PPI
MS.gene23852	MS.gene32726	PPI
MS.gene69966	MS.gene32726	PPI
MS.gene97943	MS.gene32726	PPI
MS.gene071113	MS.gene32726	PPI
MS.gene027071	MS.gene32726	PPI
MS.gene67157	MS.gene32726	PPI
MS.gene32726	MS.gene32723	PPI
MS.gene32726	MS.gene053363	PPI
MS.gene30433	MS.gene32726	PPI
MS.gene012203	MS.gene32726	PPI
MS.gene022109	MS.gene32726	PPI
MS.gene34651	MS.gene32726	PPI
MS.gene90400	MS.gene32726	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32726.t1	MTR_3g105650	95.785	427	18	0	1	427	1	427	0.0	858
MS.gene32726.t1	MTR_8g073260	44.469	461	208	9	1	427	4	450	4.63e-121	360
MS.gene32726.t1	MTR_8g072510	44.802	404	188	5	5	382	10	404	8.48e-114	341
MS.gene32726.t1	MTR_3g016210	42.299	435	200	6	3	395	8	433	2.31e-110	332
MS.gene32726.t1	MTR_8g039620	44.665	403	188	6	2	376	11	406	1.46e-108	327
MS.gene32726.t1	MTR_1g059930	52.273	308	139	2	5	311	12	312	2.48e-105	319
MS.gene32726.t1	MTR_8g062410	44.963	407	182	7	3	382	8	399	2.99e-100	306
MS.gene32726.t1	MTR_8g467780	40.323	434	212	7	1	396	7	431	4.68e-100	306
MS.gene32726.t1	MTR_8g069880	41.404	413	202	7	3	386	5	406	2.73e-99	304
MS.gene32726.t1	MTR_7g451420	49.839	311	146	3	3	311	7	309	9.16e-99	298
MS.gene32726.t1	MTR_7g021090	42.677	396	184	8	3	369	7	388	3.40e-98	300
MS.gene32726.t1	MTR_8g068990	42.932	382	168	8	5	358	12	371	5.34e-91	283
MS.gene32726.t1	MTR_8g066670	40.000	405	208	4	5	383	1075	1470	8.78e-89	295
MS.gene32726.t1	MTR_8g040930	43.175	359	164	6	48	376	2	350	1.40e-86	269
MS.gene32726.t1	MTR_8g041240	43.175	359	164	6	48	376	2	350	1.40e-86	269
MS.gene32726.t1	MTR_8g073275	35.232	474	194	14	48	427	2	456	6.03e-71	231
MS.gene32726.t1	MTR_1g115575	24.289	457	292	12	8	427	138	577	9.50e-30	122
MS.gene32726.t1	MTR_8g105260	25.059	427	265	12	40	426	101	512	1.50e-24	106
MS.gene32726.t1	MTR_7g100430	25.000	440	271	14	38	420	124	561	1.34e-21	97.8
MS.gene32726.t1	MTR_7g117620	26.688	311	189	7	40	330	119	410	1.04e-20	94.7
MS.gene32726.t1	MTR_2g096190	25.228	329	199	8	8	310	73	380	6.76e-20	92.4
MS.gene32726.t1	MTR_2g101320	27.907	215	144	2	33	246	134	338	1.16e-19	92.0
MS.gene32726.t1	MTR_1g069110	21.205	448	254	9	8	402	21	422	2.75e-19	90.1
MS.gene32726.t1	MTR_7g099580	31.148	183	113	4	14	191	21	195	1.64e-17	83.6
MS.gene32726.t1	MTR_2g101310	26.636	214	142	3	36	246	137	338	4.41e-16	80.9
MS.gene32726.t1	MTR_2g101310	26.570	207	141	2	41	246	142	338	6.73e-16	80.1
MS.gene32726.t1	MTR_4g062100	24.848	330	174	9	3	310	107	384	8.07e-16	79.7
MS.gene32726.t1	MTR_7g099570	28.796	191	122	3	8	193	12	193	1.08e-15	79.7
MS.gene32726.t1	MTR_7g100390	23.765	324	209	7	8	310	56	362	1.03e-13	73.2
MS.gene32726.t1	MTR_1g032100	20.771	467	273	11	3	426	93	505	1.45e-13	72.8
MS.gene32726.t1	MTR_5g085830	24.658	219	153	6	39	254	277	486	7.95e-13	70.9
MS.gene32726.t1	MTR_5g085810	27.094	203	132	6	56	250	320	514	9.10e-12	67.4
MS.gene32726.t1	MTR_6g027840	29.688	256	153	12	4	246	213	454	6.80e-11	64.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32726.t1	AT4G10720	40.098	409	223	6	1	391	1	405	8.68e-91	282
MS.gene32726.t1	AT4G10720	44.409	313	172	2	1	311	1	313	6.28e-85	266
MS.gene32726.t1	AT1G14500	35.922	412	237	6	1	386	1	411	7.46e-80	253
MS.gene32726.t1	AT5G15500	35.955	445	254	10	5	423	6	445	1.25e-77	248
MS.gene32726.t1	AT5G54620	34.509	397	246	5	12	402	12	400	3.17e-74	239
MS.gene32726.t1	AT5G54610	36.772	378	228	6	1	370	1	375	1.60e-73	236
MS.gene32726.t1	AT1G14480	36.132	393	228	7	1	375	1	388	4.87e-73	236
MS.gene32726.t1	AT1G14480	32.570	393	199	8	1	375	1	345	1.40e-54	186
MS.gene32726.t1	AT5G15500	30.523	344	208	10	106	423	1	339	3.36e-34	130
MS.gene32726.t1	AT5G51160	31.636	275	162	5	53	311	31	295	2.60e-32	127
MS.gene32726.t1	AT3G09550	27.346	373	225	9	4	349	146	499	9.37e-28	116
MS.gene32726.t1	AT3G09550	27.346	373	225	9	4	349	146	499	9.37e-28	116
MS.gene32726.t1	AT3G09550	27.346	373	225	9	4	349	153	506	1.03e-27	116
MS.gene32726.t1	AT3G12360	25.708	459	282	16	8	427	136	574	1.36e-23	103
MS.gene32726.t1	AT3G12360	25.708	459	282	16	8	427	124	562	1.55e-23	103
MS.gene32726.t1	AT4G03500	26.247	381	210	14	39	372	194	550	3.08e-22	99.8
MS.gene32726.t1	AT4G03500	26.697	221	132	7	2	210	80	282	4.17e-11	65.5
MS.gene32726.t1	AT4G03500	26.247	381	210	14	39	372	194	550	3.08e-22	99.8
MS.gene32726.t1	AT4G03500	26.697	221	132	7	2	210	80	282	4.17e-11	65.5
MS.gene32726.t1	AT4G03500	26.247	381	210	14	39	372	217	573	4.78e-22	99.4
MS.gene32726.t1	AT4G03500	26.697	221	132	7	2	210	103	305	4.45e-11	65.5
MS.gene32726.t1	AT4G03460	29.562	274	160	10	83	346	245	495	3.29e-21	96.7
MS.gene32726.t1	AT4G03460	29.562	274	160	10	83	346	277	527	4.14e-21	96.3
MS.gene32726.t1	AT2G01680	22.174	460	261	12	3	425	96	495	1.77e-20	94.0
MS.gene32726.t1	AT5G02620	23.007	439	276	9	8	403	63	482	4.35e-20	92.8
MS.gene32726.t1	AT5G02620	23.007	439	276	9	8	403	63	482	4.35e-20	92.8
MS.gene32726.t1	AT5G02620	23.007	439	276	9	8	403	63	482	4.35e-20	92.8
MS.gene32726.t1	AT2G31820	22.276	413	261	11	4	377	192	583	4.87e-17	84.0
MS.gene32726.t1	AT5G02620	22.511	462	273	9	8	403	63	505	6.42e-17	83.2
MS.gene32726.t1	AT5G02620	22.511	462	273	9	8	403	63	505	6.42e-17	83.2
MS.gene32726.t1	AT5G60070	23.585	318	194	6	3	309	110	389	2.92e-15	78.2
MS.gene32726.t1	AT1G05640	22.556	399	204	12	38	339	114	504	5.56e-15	77.4
MS.gene32726.t1	AT1G03670	28.182	220	128	5	97	311	279	473	5.74e-15	77.4
MS.gene32726.t1	AT1G10340	22.705	414	236	12	2	346	72	470	6.87e-15	77.0
MS.gene32726.t1	AT1G34050	23.443	273	185	7	49	311	162	420	7.39e-15	77.0
MS.gene32726.t1	AT4G03480	26.354	277	180	7	41	311	357	615	1.17e-13	73.6
MS.gene32726.t1	AT4G03480	26.354	277	180	7	41	311	360	618	1.21e-13	73.6
MS.gene32726.t1	AT4G14390	22.102	371	248	9	42	395	276	622	9.79e-12	67.4
MS.gene32726.t1	AT4G14390	22.102	371	248	9	42	395	295	641	1.25e-11	67.0
MS.gene32726.t1	AT4G03450	24.606	317	210	9	41	346	227	525	1.41e-11	67.0
MS.gene32726.t1	AT4G03450	24.606	317	210	9	41	346	227	525	2.31e-11	66.2
MS.gene32726.t1	AT4G14390	22.115	416	268	12	8	395	255	642	3.13e-11	65.9
MS.gene32726.t1	AT4G11000	28.395	162	92	4	204	345	3	160	3.57e-11	63.5
MS.gene32726.t1	AT5G50140	25.333	225	134	7	5	227	31	223	3.97e-11	65.5

Find 70 sgRNAs with CRISPR-Local

Find 121 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGCATATTGCTGCAGCTTC+TGG	0.104530	3.3:-86892487	MS.gene32726:CDS
AAAATGCGAACGTACTTCTT+AGG	0.194723	3.3:-86890784	MS.gene32726:CDS
TGTTTGTTCATCTAAATTCT+TGG	0.276195	3.3:+86891101	None:intergenic
GAACGTACTTCTTAGGGTTT+TGG	0.277692	3.3:-86890777	MS.gene32726:CDS
GACCATACATTCGAACCTTT+TGG	0.281103	3.3:+86890524	None:intergenic
TGCATATTGCTGCAGCTTCT+GGG	0.291052	3.3:-86892486	MS.gene32726:CDS
GCTTGGAAACTAAACGAGTA+TGG	0.326774	3.3:-86892418	MS.gene32726:CDS
GACTAAAACCTTCATTTGCT+TGG	0.341585	3.3:-86892435	MS.gene32726:CDS
ATTTGTGCTGCTGCATTCAA+TGG	0.351754	3.3:+86892598	None:intergenic
TTGCACATTGCTACGCAAAC+TGG	0.361427	3.3:-86892283	MS.gene32726:CDS
TTCTTGAGATCTTGCTTGGT+TGG	0.367274	3.3:-86892147	MS.gene32726:CDS
GATGTTCTTGAGATCTTGCT+TGG	0.381604	3.3:-86892151	MS.gene32726:CDS
GACCCTCTCTCCCTTTGATT+CGG	0.391340	3.3:+86892314	None:intergenic
GCCCAAAAGGTTCGAATGTA+TGG	0.400483	3.3:-86890526	MS.gene32726:CDS
TCTTGTAGTTAAATTCTTGT+CGG	0.420938	3.3:-86892251	MS.gene32726:CDS
TTTGTTTCCTTCTGAAAACT+TGG	0.426086	3.3:+86890483	None:intergenic
ATTGAATGCAGCAGCACAAA+TGG	0.428078	3.3:-86892596	MS.gene32726:CDS
AAATGCGAACGTACTTCTTA+GGG	0.430694	3.3:-86890783	MS.gene32726:CDS
ATAGCACCAAGCTATACATT+TGG	0.447152	3.3:-86890622	MS.gene32726:CDS
TTCGAATGTATGGTCGTGAA+TGG	0.451564	3.3:-86890516	MS.gene32726:CDS
TGATTTCTGCACTATAAACT+TGG	0.455664	3.3:+86891049	None:intergenic
GCTATACAAACTGATTCAAA+TGG	0.456485	3.3:-86892560	MS.gene32726:CDS
AAAGACCTTGTCCGAATCAA+AGG	0.465762	3.3:-86892325	MS.gene32726:CDS
ATACATTACATTAGCCCAAA+AGG	0.466320	3.3:-86890539	MS.gene32726:CDS
TTATAGCGTTCTAAGATGTA+TGG	0.468594	3.3:+86892535	None:intergenic
TTTAGTTTCCAAGCAAATGA+AGG	0.469820	3.3:+86892427	None:intergenic
TCTTGCTTGGTTGGCTTCGA+AGG	0.470106	3.3:-86892138	MS.gene32726:CDS
AAATTCTTGTCGGTTTGTCC+AGG	0.494151	3.3:-86892241	MS.gene32726:CDS
TGTCATATTGTGCAGGCAGT+TGG	0.496735	3.3:-86891156	MS.gene32726:intron
CTCTCCCTTTGATTCGGACA+AGG	0.497968	3.3:+86892320	None:intergenic
GATCGTAGCTCTTCGTGAAG+TGG	0.498077	3.3:+86890973	None:intergenic
TGCAAAGGAGTATCAACAAA+TGG	0.503702	3.3:+86892505	None:intergenic
ATAACACTTATACGTCCTCT+TGG	0.503876	3.3:+86890700	None:intergenic
AGTTTATAGTGCAGAAATCA+AGG	0.506788	3.3:-86891046	MS.gene32726:CDS
TTTGTGCTGCTGCATTCAAT+GGG	0.507115	3.3:+86892599	None:intergenic
TTTAGATGAACAAACAGCAT+TGG	0.510720	3.3:-86891094	MS.gene32726:CDS
AGCTGGTGGAGGAAAATTAG+TGG	0.517201	3.3:-86890812	MS.gene32726:CDS
CTGAATTGGGAGGATGAAGC+AGG	0.520550	3.3:-86892076	MS.gene32726:CDS
TCTTAAACGAGTAACACAAA+TGG	0.523638	3.3:+86890924	None:intergenic
AAGGACATGTTGATTAAAGC+AGG	0.524636	3.3:-86891027	MS.gene32726:CDS
ATTAAAGCAGGAGCTTTACA+TGG	0.524711	3.3:-86891015	MS.gene32726:CDS
TACACTGCCACAAGTATACA+TGG	0.526508	3.3:+86890580	None:intergenic
TGTCCACTTGCTTTATTCTG+TGG	0.533431	3.3:-86890670	MS.gene32726:CDS
ACAAAACAAATATTACAGAA+TGG	0.533575	3.3:-86892371	MS.gene32726:CDS
ACAATTCCAAATGTATAGCT+TGG	0.534815	3.3:+86890616	None:intergenic
TTGAATGCAGCAGCACAAAT+GGG	0.536468	3.3:-86892595	MS.gene32726:CDS
TCGAATGTATGGTCGTGAAT+GGG	0.544937	3.3:-86890515	MS.gene32726:CDS
AAGACCTTGTCCGAATCAAA+GGG	0.554193	3.3:-86892324	MS.gene32726:CDS
GCTTGGTGCTATAACTAGCA+TGG	0.562144	3.3:+86890633	None:intergenic
TATTGAGAAGTGACATGTGC+AGG	0.574312	3.3:+86892044	None:intergenic
GGCTATTCCATGTATACTTG+TGG	0.575896	3.3:-86890587	MS.gene32726:CDS
CTTAACCCTCCAGCTGGTGG+AGG	0.584385	3.3:-86890823	MS.gene32726:CDS
CCTTGTGATGAGAACGTCTG+TGG	0.589242	3.3:+86892110	None:intergenic
CAACCACAGAATAAAGCAAG+TGG	0.589426	3.3:+86890667	None:intergenic
CCACAGACGTTCTCATCACA+AGG	0.592862	3.3:-86892110	MS.gene32726:CDS
AGCTCTTCGTGAAGTGGAGT+AGG	0.598129	3.3:+86890979	None:intergenic
ACAAACAGCATTGGACATAG+TGG	0.603971	3.3:-86891085	MS.gene32726:CDS
GTCCGAATCAAAGGGAGAGA+GGG	0.604625	3.3:-86892316	MS.gene32726:CDS
ACACTTAACCCTCCAGCTGG+TGG	0.610202	3.3:-86890826	MS.gene32726:CDS
GAAGCTGCAGCAATATGCAA+AGG	0.614052	3.3:+86892490	None:intergenic
TGTCCGAATCAAAGGGAGAG+AGG	0.614649	3.3:-86892317	MS.gene32726:CDS
ACTTCTCAATAGCTTCCCAC+AGG	0.615391	3.3:-86892032	MS.gene32726:intron
GTGACATCTTCAATACAACC+TGG	0.619746	3.3:+86892223	None:intergenic
GCAACACTTAACCCTCCAGC+TGG	0.624740	3.3:-86890829	MS.gene32726:CDS
GTTTGCGTAGCAATGTGCAA+AGG	0.652449	3.3:+86892286	None:intergenic
ATGTGTTTATTAATGCCAAG+AGG	0.652670	3.3:-86890715	MS.gene32726:CDS
GAGAAGTGACATGTGCAGGA+TGG	0.692918	3.3:+86892048	None:intergenic
AAAACGGGTTCTGAATTGGG+AGG	0.707905	3.3:-86892086	MS.gene32726:CDS
TACATTTGGAATTGTCACTG+TGG	0.721071	3.3:-86890608	MS.gene32726:CDS
GGTCTTTATTAATGTCTACG+AGG	0.768754	3.3:+86892341	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AAAATATTAGTTTTAAATTG+AGG	+	chr3.3:86891647-86891666	None:intergenic	10.0%
!!	CTTATTTATAAGAGATATTT+AGG	+	chr3.3:86891760-86891779	None:intergenic	15.0%
!!	TATATTAATTAATTCAGCTA+TGG	-	chr3.3:86891829-86891848	MS.gene32726:intron	15.0%
!!!	GTTTGTTATAATTTTTAAAG+TGG	-	chr3.3:86891576-86891595	MS.gene32726:intron	15.0%
!!!	TATTTACTCATGTTTTTAAA+AGG	-	chr3.3:86891434-86891453	MS.gene32726:intron	15.0%
!!!	TTTAAAACTAATATTTTCTC+TGG	-	chr3.3:86891650-86891669	MS.gene32726:intron	15.0%
!!!	TTTTGATTTGACAATTAAAT+TGG	-	chr3.3:86891542-86891561	MS.gene32726:intron	15.0%
!!	ACAAAACAAATATTACAGAA+TGG	-	chr3.3:86890713-86890732	MS.gene32726:CDS	20.0%
!!	AGTAGAAAGAAAAGAAATAT+AGG	+	chr3.3:86891091-86891110	None:intergenic	20.0%
!!	GTAGAAAGAAAAGAAATATA+GGG	+	chr3.3:86891090-86891109	None:intergenic	20.0%
!!!	AATATTTGTTTTGTAAAGCA+AGG	+	chr3.3:86890707-86890726	None:intergenic	20.0%
!!!	ATATGCCTTTAAAATACTTA+GGG	-	chr3.3:86891158-86891177	MS.gene32726:intron	20.0%
!!!	CTTGTTTTTCTTTTGTATTA+CGG	-	chr3.3:86891218-86891237	MS.gene32726:intron	20.0%
!!!	TATATGCCTTTAAAATACTT+AGG	-	chr3.3:86891157-86891176	MS.gene32726:intron	20.0%
!	AAACTAAATTAACTCAGCTA+TGG	-	chr3.3:86891902-86891921	MS.gene32726:intron	25.0%
!	CACATAAATATCTACAAATG+TGG	-	chr3.3:86891115-86891134	MS.gene32726:CDS	25.0%
!	TCTTGTAGTTAAATTCTTGT+CGG	-	chr3.3:86890833-86890852	MS.gene32726:CDS	25.0%
!	TGTTTGTTCATCTAAATTCT+TGG	+	chr3.3:86891986-86892005	None:intergenic	25.0%
!	TTGTTTGATCTTCAATGTTA+TGG	+	chr3.3:86891398-86891417	None:intergenic	25.0%
!!!	AAGTACCCTAAGTATTTTAA+AGG	+	chr3.3:86891166-86891185	None:intergenic	25.0%
	AACTAAGGGTCTCTTTATAA+GGG	+	chr3.3:86891356-86891375	None:intergenic	30.0%
	ACAATTCCAAATGTATAGCT+TGG	+	chr3.3:86892471-86892490	None:intergenic	30.0%
	AGTTTATAGTGCAGAAATCA+AGG	-	chr3.3:86892038-86892057	MS.gene32726:CDS	30.0%
	ATACATTACATTAGCCCAAA+AGG	-	chr3.3:86892545-86892564	MS.gene32726:CDS	30.0%
	ATGTGTTTATTAATGCCAAG+AGG	-	chr3.3:86892369-86892388	MS.gene32726:CDS	30.0%
	TCTTAAACGAGTAACACAAA+TGG	+	chr3.3:86892163-86892182	None:intergenic	30.0%
	TGATTTCTGCACTATAAACT+TGG	+	chr3.3:86892038-86892057	None:intergenic	30.0%
	TTTAGATGAACAAACAGCAT+TGG	-	chr3.3:86891990-86892009	MS.gene32726:intron	30.0%
!	AGTTTTCAGAAGGAAACAAA+TGG	-	chr3.3:86892604-86892623	MS.gene32726:CDS	30.0%
!	GCTATACAAACTGATTCAAA+TGG	-	chr3.3:86890524-86890543	MS.gene32726:CDS	30.0%
!	TTTAGTTTCCAAGCAAATGA+AGG	+	chr3.3:86890660-86890679	None:intergenic	30.0%
!	TTTGTTTCCTTCTGAAAACT+TGG	+	chr3.3:86892604-86892623	None:intergenic	30.0%
!	TTTTAGTCTCATAATCTCAG+TGG	+	chr3.3:86890637-86890656	None:intergenic	30.0%
!	TTTTGTAAAGCAAGGTGTAT+GGG	+	chr3.3:86890699-86890718	None:intergenic	30.0%
!!	TCAATCAAACTACTAGTCTT+TGG	+	chr3.3:86891784-86891803	None:intergenic	30.0%
!!	TTATAGCGTTCTAAGATGTA+TGG	+	chr3.3:86890552-86890571	None:intergenic	30.0%
!!!	GGTACTTTTTTGAAGAGTTT+TGG	-	chr3.3:86891179-86891198	MS.gene32726:intron	30.0%
!!!	TTTTTTGAAGAGTTTTGGAG+AGG	-	chr3.3:86891184-86891203	MS.gene32726:intron	30.0%
	AAAATGCGAACGTACTTCTT+AGG	-	chr3.3:86892300-86892319	MS.gene32726:CDS	35.0%
	AAATGCGAACGTACTTCTTA+GGG	-	chr3.3:86892301-86892320	MS.gene32726:CDS	35.0%
	ACTAAGGGTCTCTTTATAAG+GGG	+	chr3.3:86891355-86891374	None:intergenic	35.0%
	ATAACACTTATACGTCCTCT+TGG	+	chr3.3:86892387-86892406	None:intergenic	35.0%
	ATAGCACCAAGCTATACATT+TGG	-	chr3.3:86892462-86892481	MS.gene32726:CDS	35.0%
	ATTAAAGCAGGAGCTTTACA+TGG	-	chr3.3:86892069-86892088	MS.gene32726:CDS	35.0%
	CAACTAAGGGTCTCTTTATA+AGG	+	chr3.3:86891357-86891376	None:intergenic	35.0%
	GACTAAAACCTTCATTTGCT+TGG	-	chr3.3:86890649-86890668	MS.gene32726:CDS	35.0%
	GGTCTTTATTAATGTCTACG+AGG	+	chr3.3:86890746-86890765	None:intergenic	35.0%
	GTTCTCATCACAAGGAAAAA+CGG	-	chr3.3:86890982-86891001	MS.gene32726:CDS	35.0%
	TAAAGAGACCCTTAGTTGTT+TGG	-	chr3.3:86891359-86891378	MS.gene32726:intron	35.0%
	TTCTCATCACAAGGAAAAAC+GGG	-	chr3.3:86890983-86891002	MS.gene32726:CDS	35.0%
!	AAGGACATGTTGATTAAAGC+AGG	-	chr3.3:86892057-86892076	MS.gene32726:CDS	35.0%
!	ACCATACATTCGAACCTTTT+GGG	+	chr3.3:86892562-86892581	None:intergenic	35.0%
!	ACTTGTGGCAGTGTATTTTT+GGG	-	chr3.3:86892512-86892531	MS.gene32726:CDS	35.0%
!	GATGTATCCAAGTTTTCAGA+AGG	-	chr3.3:86892594-86892613	MS.gene32726:CDS	35.0%
!	GTTTTGTAAAGCAAGGTGTA+TGG	+	chr3.3:86890700-86890719	None:intergenic	35.0%
!	TACATTTGGAATTGTCACTG+TGG	-	chr3.3:86892476-86892495	MS.gene32726:CDS	35.0%
!	TACTTGTGGCAGTGTATTTT+TGG	-	chr3.3:86892511-86892530	MS.gene32726:CDS	35.0%
!!	TGCAAAGGAGTATCAACAAA+TGG	+	chr3.3:86890582-86890601	None:intergenic	35.0%
	AAAGACCTTGTCCGAATCAA+AGG	-	chr3.3:86890759-86890778	MS.gene32726:CDS	40.0%
	AAGACCTTGTCCGAATCAAA+GGG	-	chr3.3:86890760-86890779	MS.gene32726:CDS	40.0%
	ATCTCGAGACCAAACAACTA+AGG	+	chr3.3:86891371-86891390	None:intergenic	40.0%
	ATGGTCATGTCATATTGTGC+AGG	-	chr3.3:86891921-86891940	MS.gene32726:intron	40.0%
	ATTGAATGCAGCAGCACAAA+TGG	-	chr3.3:86890488-86890507	MS.gene32726:CDS	40.0%
	CAACCACAGAATAAAGCAAG+TGG	+	chr3.3:86892420-86892439	None:intergenic	40.0%
	CTAAGGGTCTCTTTATAAGG+GGG	+	chr3.3:86891354-86891373	None:intergenic	40.0%
	GATGTTCTTGAGATCTTGCT+TGG	-	chr3.3:86890933-86890952	MS.gene32726:CDS	40.0%
	GCTTGGAAACTAAACGAGTA+TGG	-	chr3.3:86890666-86890685	MS.gene32726:CDS	40.0%
	GGCTATTCCATGTATACTTG+TGG	-	chr3.3:86892497-86892516	MS.gene32726:CDS	40.0%
	GTGACATCTTCAATACAACC+TGG	+	chr3.3:86890864-86890883	None:intergenic	40.0%
	TAAGGGTCTCTTTATAAGGG+GGG	+	chr3.3:86891353-86891372	None:intergenic	40.0%
	TACACTGCCACAAGTATACA+TGG	+	chr3.3:86892507-86892526	None:intergenic	40.0%
	TATTGAGAAGTGACATGTGC+AGG	+	chr3.3:86891043-86891062	None:intergenic	40.0%
	TCGAATGTATGGTCGTGAAT+GGG	-	chr3.3:86892569-86892588	MS.gene32726:CDS	40.0%
	TCTCGAGACCAAACAACTAA+GGG	+	chr3.3:86891370-86891389	None:intergenic	40.0%
	TTCGAATGTATGGTCGTGAA+TGG	-	chr3.3:86892568-86892587	MS.gene32726:CDS	40.0%
	TTCTTGAGATCTTGCTTGGT+TGG	-	chr3.3:86890937-86890956	MS.gene32726:CDS	40.0%
	TTGAATGCAGCAGCACAAAT+GGG	-	chr3.3:86890489-86890508	MS.gene32726:CDS	40.0%
!	ACAAACAGCATTGGACATAG+TGG	-	chr3.3:86891999-86892018	MS.gene32726:intron	40.0%
!	ATTTGTGCTGCTGCATTCAA+TGG	+	chr3.3:86890489-86890508	None:intergenic	40.0%
!	GAACGTACTTCTTAGGGTTT+TGG	-	chr3.3:86892307-86892326	MS.gene32726:CDS	40.0%
!	GACCATACATTCGAACCTTT+TGG	+	chr3.3:86892563-86892582	None:intergenic	40.0%
!	TGTCCACTTGCTTTATTCTG+TGG	-	chr3.3:86892414-86892433	MS.gene32726:CDS	40.0%
!	TTTGTGCTGCTGCATTCAAT+GGG	+	chr3.3:86890488-86890507	None:intergenic	40.0%
!!	AAATTCTTGTCGGTTTGTCC+AGG	-	chr3.3:86890843-86890862	MS.gene32726:CDS	40.0%
!!	AGGAAAAACGGGTTCTGAAT+TGG	-	chr3.3:86890994-86891013	MS.gene32726:CDS	40.0%
!!	CTTGTGGCAGTGTATTTTTG+GGG	-	chr3.3:86892513-86892532	MS.gene32726:CDS	40.0%
!!	GGAAAAACGGGTTCTGAATT+GGG	-	chr3.3:86890995-86891014	MS.gene32726:CDS	40.0%
!!!	TTTGAAGAGTTTTGGAGAGG+AGG	-	chr3.3:86891187-86891206	MS.gene32726:intron	40.0%
	ACTTCTCAATAGCTTCCCAC+AGG	-	chr3.3:86891052-86891071	MS.gene32726:CDS	45.0%
	AGCTGGTGGAGGAAAATTAG+TGG	-	chr3.3:86892272-86892291	MS.gene32726:CDS	45.0%
	CTTTATAAGGGGGGTCTGAA+GGG	+	chr3.3:86891344-86891363	None:intergenic	45.0%
	GAAGCTGCAGCAATATGCAA+AGG	+	chr3.3:86890597-86890616	None:intergenic	45.0%
	GCCCAAAAGGTTCGAATGTA+TGG	-	chr3.3:86892558-86892577	MS.gene32726:CDS	45.0%
	GTTTGCGTAGCAATGTGCAA+AGG	+	chr3.3:86890801-86890820	None:intergenic	45.0%
	TCTTTATAAGGGGGGTCTGA+AGG	+	chr3.3:86891345-86891364	None:intergenic	45.0%
	TGCATATTGCTGCAGCTTCT+GGG	-	chr3.3:86890598-86890617	MS.gene32726:CDS	45.0%
	TGTCATATTGTGCAGGCAGT+TGG	-	chr3.3:86891928-86891947	MS.gene32726:intron	45.0%
	TTGCACATTGCTACGCAAAC+TGG	-	chr3.3:86890801-86890820	MS.gene32726:CDS	45.0%
	TTGCATATTGCTGCAGCTTC+TGG	-	chr3.3:86890597-86890616	MS.gene32726:CDS	45.0%
	TTTATAAGGGGGGTCTGAAG+GGG	+	chr3.3:86891343-86891362	None:intergenic	45.0%
!	ACTAATTTTCCTCCACCAGC+TGG	+	chr3.3:86892273-86892292	None:intergenic	45.0%
!!	AAAACGGGTTCTGAATTGGG+AGG	-	chr3.3:86890998-86891017	MS.gene32726:CDS	45.0%
!!	GCTTGGTGCTATAACTAGCA+TGG	+	chr3.3:86892454-86892473	None:intergenic	45.0%
	AGCTCTTCGTGAAGTGGAGT+AGG	+	chr3.3:86892108-86892127	None:intergenic	50.0%
	AGGGATGTGTCTCATACCTG+TGG	+	chr3.3:86891071-86891090	None:intergenic	50.0%
	CCACAGACGTTCTCATCACA+AGG	-	chr3.3:86890974-86890993	MS.gene32726:CDS	50.0%
	CCTTGTGATGAGAACGTCTG+TGG	+	chr3.3:86890977-86890996	None:intergenic	50.0%
	CTGAATTGGGAGGATGAAGC+AGG	-	chr3.3:86891008-86891027	MS.gene32726:CDS	50.0%
	GACCCTCTCTCCCTTTGATT+CGG	+	chr3.3:86890773-86890792	None:intergenic	50.0%
	GAGAAGTGACATGTGCAGGA+TGG	+	chr3.3:86891039-86891058	None:intergenic	50.0%
	GATCGTAGCTCTTCGTGAAG+TGG	+	chr3.3:86892114-86892133	None:intergenic	50.0%
	GGGATGTGTCTCATACCTGT+GGG	+	chr3.3:86891070-86891089	None:intergenic	50.0%
	GTCCGAATCAAAGGGAGAGA+GGG	-	chr3.3:86890768-86890787	MS.gene32726:CDS	50.0%
	TGTCCGAATCAAAGGGAGAG+AGG	-	chr3.3:86890767-86890786	MS.gene32726:CDS	50.0%
!	AATTTTCCTCCACCAGCTGG+AGG	+	chr3.3:86892270-86892289	None:intergenic	50.0%
!	ATTTTCCTCCACCAGCTGGA+GGG	+	chr3.3:86892269-86892288	None:intergenic	50.0%
!!	CTCTCCCTTTGATTCGGACA+AGG	+	chr3.3:86890767-86890786	None:intergenic	50.0%
!!	TCTTGCTTGGTTGGCTTCGA+AGG	-	chr3.3:86890946-86890965	MS.gene32726:CDS	50.0%
	ACACTTAACCCTCCAGCTGG+TGG	-	chr3.3:86892258-86892277	MS.gene32726:CDS	55.0%
	GCAACACTTAACCCTCCAGC+TGG	-	chr3.3:86892255-86892274	MS.gene32726:CDS	55.0%
	CTTAACCCTCCAGCTGGTGG+AGG	-	chr3.3:86892261-86892280	MS.gene32726:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.3	gene	86890477	86892629	86890477	ID=MS.gene32726
chr3.3	mRNA	86890477	86892629	86890477	ID=MS.gene32726.t1;Parent=MS.gene32726
chr3.3	exon	86892033	86892629	86892033	ID=MS.gene32726.t1.exon1;Parent=MS.gene32726.t1
chr3.3	CDS	86892033	86892629	86892033	ID=cds.MS.gene32726.t1;Parent=MS.gene32726.t1
chr3.3	exon	86890477	86891163	86890477	ID=MS.gene32726.t1.exon2;Parent=MS.gene32726.t1
chr3.3	CDS	86890477	86891163	86890477	ID=cds.MS.gene32726.t1;Parent=MS.gene32726.t1

Gene Sequence

>MS.gene32726

ATGAATGACCCATTGAATGCAGCAGCACAAATGGGAGATATAGACTTGCTATACAAACTGATTCAAATGGATCCATACATCTTAGAACGCTATAATTTAATACCATTTGTTGATACTCCTTTGCATATTGCTGCAGCTTCTGGGCATGTAAGTTTTGCCACTGAGATTATGAGACTAAAACCTTCATTTGCTTGGAAACTAAACGAGTATGGACTCAGTCCCATACACCTTGCTTTACAAAACAAATATTACAGAATGGTGTGTCGCCTCGTAGACATTAATAAAGACCTTGTCCGAATCAAAGGGAGAGAGGGTCTAACTCCTTTGCACATTGCTACGCAAACTGGAAACTTTGATCTTGTAGTTAAATTCTTGTCGGTTTGTCCAGGTTGTATTGAAGATGTCACAGTTCGAAGTGAGACTGCTTTGCATCTTGCTGTGAAAGATAAGCAGTTTGATGTTCTTGAGATCTTGCTTGGTTGGCTTCGAAGGACATGCCACAGACGTTCTCATCACAAGGAAAAACGGGTTCTGAATTGGGAGGATGAAGCAGGCAACACCATCCTGCACATGTCACTTCTCAATAGCTTCCCACAGGTATGAGACACATCCCTATATTTCTTTTCTTTCTACTTTTTCACATAAATATCTACAAATGTGGCTGATGCGACTGCAATGCATATATGCCTTTAAAATACTTAGGGTACTTTTTTGAAGAGTTTTGGAGAGGAGGAGAAGAGACTTGTTTTTCTTTTGTATTACGGACACTTTAAAATATTTTTTAAAATCAATCAAAAGTTGAAGTATCGTATGATCATTTATCATTTTTTTGAATAACAAATTTATATTAAAATATAGACTTACCCCTTCAGACCCCCCTTATAAAGAGACCCTTAGTTGTTTGGTCTCGAGATATTTCCATAACATTGAAGATCAAACAAAGTTTTATGTAATCGATATTTACTCATGTTTTTAAAAGGATAATATACATTTATTTGATACTATTTAATAAATATCAAATTTTGAAATTTAATAAAATTTTGTTAAATTAATCAACTCAATGACTTTTGATTTGACAATTAAATTGGTGAAATTCAGTGTTTGTTATAATTTTTAAAGTGGAGTAACTCAGTTGAGAGTTAATCTTAGAGCGATTGCAGTTACGTACCTCAATTTAAAACTAATATTTTCTCTGGTCTTATTTATAAGAGACATTTGAGTCAATAAAAGTTAGATGTATCTGATTAAGATTTTGAATTAGATAGATCAACTTTTGTTGACCTAAATATCTCTTATAAATAAGACCAAAGACTAGTAGTTTGATTGAAAATCAAAATCAATCTAAGTTTCAATATATTAATTAATTCAGCTATGGTGTAGTTTGACATTTTCAAATTATTAATAATTTTTCTCTTCTATTTAAAAAAACTAAATTAACTCAGCTATGGTCATGTCATATTGTGCAGGCAGTTGGATTGTTGATAGATAGCAACATAGACATAAATGCCAAGAATTTAGATGAACAAACAGCATTGGACATAGTGGAGCAGATTCAGAGCCAAGTTTATAGTGCAGAAATCAAGGACATGTTGATTAAAGCAGGAGCTTTACATGGTTTTTCACTTGCTCCTACTCCACTTCACGAAGAGCTACGATCAAAAATCACATTTAACGAGAGAATAGCCATTTGTGTTACTCGTTTAAGAAGAAGAATTTCAAATGACACAAGAAACGCTTTGTTAGTAGTTGCTATTCTTTTTGCAACAAGTGCATATGAAGCAACACTTAACCCTCCAGCTGGTGGAGGAAAATTAGTGGTGATGAAAATGCGAACGTACTTCTTAGGGTTTTGGTCGATTAATACATTTTCATTTTATGTTTCAGTTTTGATGATGTGTTTATTAATGCCAAGAGGACGTATAAGTGTTATAGTAACTTGTCCACTTGCTTTATTCTGTGGTTGTTATATGTTTTCCATGCTAGTTATAGCACCAAGCTATACATTTGGAATTGTCACTGTGGCTATTCCATGTATACTTGTGGCAGTGTATTTTTGGGGAGCTATGATATACATTACATTAGCCCAAAAGGTTCGAATGTATGGTCGTGAATGGGAAGATGTATCCAAGTTTTCAGAAGGAAACAAATGGTAA

Protein sequence

>MS.gene32726.t1

MNDPLNAAAQMGDIDLLYKLIQMDPYILERYNLIPFVDTPLHIAAASGHVSFATEIMRLKPSFAWKLNEYGLSPIHLALQNKYYRMVCRLVDINKDLVRIKGREGLTPLHIATQTGNFDLVVKFLSVCPGCIEDVTVRSETALHLAVKDKQFDVLEILLGWLRRTCHRRSHHKEKRVLNWEDEAGNTILHMSLLNSFPQAVGLLIDSNIDINAKNLDEQTALDIVEQIQSQVYSAEIKDMLIKAGALHGFSLAPTPLHEELRSKITFNERIAICVTRLRRRISNDTRNALLVVAILFATSAYEATLNPPAGGGKLVVMKMRTYFLGFWSINTFSFYVSVLMMCLLMPRGRISVIVTCPLALFCGCYMFSMLVIAPSYTFGIVTVAIPCILVAVYFWGAMIYITLAQKVRMYGREWEDVSKFSEGNKW