AlfalfaGEDB | MS.gene042783

M. sativa cultivar XinJiangDaYe / MS.gene042783

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF12796.10 PF13962.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene042783.t1	RHN41590.1	69.2	406	121	2	1	403	1	405	3.50E-126	461.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene042783.t1	Q8GYH5	40.5	304	172	3	10	306	3	304	5.8e-21	103.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene042783.t1	A0A396GKQ3	69.2	406	121	2	1	403	1	405	2.5e-126	461.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene005995	MS.gene042783	PPI
MS.gene042783	MS.gene90400	PPI
MS.gene037950	MS.gene042783	PPI
MS.gene068249	MS.gene042783	PPI
MS.gene051003	MS.gene042783	PPI
MS.gene042783	MS.gene09785	PPI
MS.gene64441	MS.gene042783	PPI
MS.gene24806	MS.gene042783	PPI
MS.gene042783	MS.gene81055	PPI
MS.gene30176	MS.gene042783	PPI
MS.gene042783	MS.gene80163	PPI
MS.gene042783	MS.gene33377	PPI
MS.gene042783	MS.gene027072	PPI
MS.gene007487	MS.gene042783	PPI
MS.gene042783	MS.gene65463	PPI
MS.gene042783	MS.gene007485	PPI
MS.gene066464	MS.gene042783	PPI
MS.gene042783	MS.gene87218	PPI
MS.gene042783	MS.gene025229	PPI
MS.gene24922	MS.gene042783	PPI
MS.gene066467	MS.gene042783	PPI
MS.gene47479	MS.gene042783	PPI
MS.gene042783	MS.gene043742	PPI
MS.gene042783	MS.gene027075	PPI
MS.gene042783	MS.gene027441	PPI
MS.gene042783	MS.gene050457	PPI
MS.gene042783	MS.gene99973	PPI
MS.gene008767	MS.gene042783	PPI
MS.gene042783	MS.gene008833	PPI
MS.gene36842	MS.gene042783	PPI
MS.gene042783	MS.gene064542	PPI
MS.gene99554	MS.gene042783	PPI
MS.gene042783	MS.gene48655	PPI
MS.gene066046	MS.gene042783	PPI
MS.gene41291	MS.gene042783	PPI
MS.gene042783	MS.gene027073	PPI
MS.gene042783	MS.gene34712	PPI
MS.gene22482	MS.gene042783	PPI
MS.gene042783	MS.gene037184	PPI
MS.gene042783	MS.gene23196	PPI
MS.gene039682	MS.gene042783	PPI
MS.gene061932	MS.gene042783	PPI
MS.gene042783	MS.gene067705	PPI
MS.gene042783	MS.gene038519	PPI
MS.gene26532	MS.gene042783	PPI
MS.gene21734	MS.gene042783	PPI
MS.gene71204	MS.gene042783	PPI
MS.gene042783	MS.gene26021	PPI
MS.gene007486	MS.gene042783	PPI
MS.gene041770	MS.gene042783	PPI
MS.gene042783	MS.gene050456	PPI
MS.gene042783	MS.gene51258	PPI
MS.gene97379	MS.gene042783	PPI
MS.gene042783	MS.gene053363	PPI
MS.gene042783	MS.gene91775	PPI
MS.gene042783	MS.gene25515	PPI
MS.gene058128	MS.gene042783	PPI
MS.gene042783	MS.gene34651	PPI
MS.gene066501	MS.gene042783	PPI
MS.gene25244	MS.gene042783	PPI
MS.gene042783	MS.gene32726	PPI
MS.gene08470	MS.gene042783	PPI
MS.gene63085	MS.gene042783	PPI
MS.gene042783	MS.gene050270	PPI
MS.gene056455	MS.gene042783	PPI
MS.gene042783	MS.gene052956	PPI
MS.gene042783	MS.gene60920	PPI
MS.gene042783	MS.gene73119	PPI
MS.gene042783	MS.gene92243	PPI
MS.gene89986	MS.gene042783	PPI
MS.gene042783	MS.gene34009	PPI
MS.gene035805	MS.gene042783	PPI
MS.gene042783	MS.gene018518	PPI
MS.gene042783	MS.gene043662	PPI
MS.gene042783	MS.gene065848	PPI
MS.gene042783	MS.gene004844	PPI
MS.gene042783	MS.gene99588	PPI
MS.gene042783	MS.gene21888	PPI
MS.gene69965	MS.gene042783	PPI
MS.gene042783	MS.gene54764	PPI
MS.gene042783	MS.gene27223	PPI
MS.gene82740	MS.gene042783	PPI
MS.gene042783	MS.gene39623	PPI
MS.gene042783	MS.gene25024	PPI
MS.gene042783	MS.gene26019	PPI
MS.gene042783	MS.gene022109	PPI
MS.gene042783	MS.gene050272	PPI
MS.gene30172	MS.gene042783	PPI
MS.gene23852	MS.gene042783	PPI
MS.gene69966	MS.gene042783	PPI
MS.gene97943	MS.gene042783	PPI
MS.gene071113	MS.gene042783	PPI
MS.gene042783	MS.gene65775	PPI
MS.gene042783	MS.gene32723	PPI
MS.gene027071	MS.gene042783	PPI
MS.gene042783	MS.gene02469	PPI
MS.gene67157	MS.gene042783	PPI
MS.gene042783	MS.gene30174	PPI
MS.gene30433	MS.gene042783	PPI
MS.gene042783	MS.gene045937	PPI
MS.gene012203	MS.gene042783	PPI
MS.gene010910	MS.gene042783	PPI
MS.gene042783	MS.gene71122	PPI
MS.gene042783	MS.gene51278	PPI
MS.gene063222	MS.gene042783	PPI
MS.gene042783	MS.gene85534	PPI
MS.gene07706	MS.gene042783	PPI
MS.gene027074	MS.gene042783	PPI
MS.gene042783	MS.gene51262	PPI
MS.gene042783	MS.gene23881	PPI
MS.gene69687	MS.gene042783	PPI
MS.gene042783	MS.gene26672	PPI
MS.gene010912	MS.gene042783	PPI
MS.gene042783	MS.gene014931	PPI
MS.gene042783	MS.gene062662	PPI
MS.gene042783	MS.gene97379	PPI
MS.gene23811	MS.gene042783	PPI
MS.gene23810	MS.gene042783	PPI
MS.gene042783	MS.gene066500	PPI
MS.gene042783	MS.gene30177	PPI
MS.gene042783	MS.gene70008	PPI
MS.gene51335	MS.gene042783	PPI
MS.gene024753	MS.gene042783	PPI
MS.gene78281	MS.gene042783	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene042783.t1	MTR_8g039620	69.903	412	118	3	1	406	1	412	0.0	557
MS.gene042783.t1	MTR_8g072510	66.751	397	132	0	11	407	9	405	0.0	532
MS.gene042783.t1	MTR_8g068990	68.992	387	100	4	1	384	1	370	0.0	529
MS.gene042783.t1	MTR_1g059930	71.129	381	109	1	1	380	1	381	1.48e-177	503
MS.gene042783.t1	MTR_8g040930	70.255	353	102	1	54	403	1	353	5.46e-175	495
MS.gene042783.t1	MTR_8g041240	70.255	353	102	1	54	403	1	353	5.46e-175	495
MS.gene042783.t1	MTR_8g062410	60.138	434	154	6	10	433	8	432	5.06e-172	490
MS.gene042783.t1	MTR_3g016210	59.338	423	151	4	10	416	8	425	5.04e-168	479
MS.gene042783.t1	MTR_7g021090	60.298	403	139	6	1	395	1	390	1.45e-166	474
MS.gene042783.t1	MTR_8g069880	57.309	431	180	3	12	439	7	436	2.21e-156	451
MS.gene042783.t1	MTR_8g073260	55.528	398	170	3	11	405	10	403	2.14e-149	432
MS.gene042783.t1	MTR_7g451420	66.450	307	103	0	9	315	6	312	1.66e-139	402
MS.gene042783.t1	MTR_8g467780	54.197	417	177	5	10	413	9	424	2.79e-135	397
MS.gene042783.t1	MTR_8g066670	52.765	434	192	6	9	435	1072	1499	1.64e-133	419
MS.gene042783.t1	MTR_3g105650	45.497	433	198	6	12	434	5	409	3.42e-115	344
MS.gene042783.t1	MTR_8g073275	41.394	459	190	9	54	443	1	449	3.91e-97	299
MS.gene042783.t1	MTR_1g069110	26.150	413	261	12	15	409	21	407	2.11e-28	117
MS.gene042783.t1	MTR_7g099580	25.743	303	168	8	21	315	21	274	2.42e-22	97.8
MS.gene042783.t1	MTR_4g062100	24.872	390	223	11	8	362	70	424	4.89e-18	87.0
MS.gene042783.t1	MTR_2g101320	26.840	231	147	4	40	260	134	352	1.75e-17	85.5
MS.gene042783.t1	MTR_1g115575	23.429	350	204	9	47	369	169	481	3.04e-17	84.7
MS.gene042783.t1	MTR_7g099570	27.670	206	137	2	12	213	9	206	7.28e-17	83.6
MS.gene042783.t1	MTR_7g117620	26.481	287	177	6	47	311	119	393	1.67e-16	82.0
MS.gene042783.t1	MTR_2g101310	28.169	213	135	4	43	247	137	339	2.38e-15	79.0
MS.gene042783.t1	MTR_2g101310	28.169	213	135	4	43	247	137	339	3.40e-15	77.8
MS.gene042783.t1	MTR_1g032100	21.777	349	215	9	47	368	94	411	4.20e-14	74.7
MS.gene042783.t1	MTR_2g096190	22.314	363	231	11	8	362	101	420	1.81e-13	72.8
MS.gene042783.t1	MTR_8g105260	21.552	348	217	7	47	368	101	418	1.62e-12	69.7
MS.gene042783.t1	MTR_7g100390	22.751	378	210	11	8	362	84	402	2.55e-11	65.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene042783.t1	AT4G10720	36.930	417	240	5	11	417	4	407	7.81e-83	262
MS.gene042783.t1	AT4G10720	36.190	420	240	6	11	416	4	409	6.71e-78	248
MS.gene042783.t1	AT5G15500	35.520	442	261	10	12	443	6	433	3.42e-73	237
MS.gene042783.t1	AT1G14480	34.071	452	251	12	11	443	4	427	2.26e-71	232
MS.gene042783.t1	AT1G14500	32.941	425	244	7	11	420	4	402	6.11e-70	228
MS.gene042783.t1	AT5G54620	40.850	306	174	2	11	309	4	309	5.35e-67	220
MS.gene042783.t1	AT5G54610	38.158	304	179	3	10	306	3	304	2.60e-63	211
MS.gene042783.t1	AT1G14480	31.663	439	236	12	11	443	4	384	6.08e-55	188
MS.gene042783.t1	AT5G15500	30.792	341	212	10	113	443	1	327	4.58e-34	130
MS.gene042783.t1	AT5G51160	26.753	385	246	9	60	418	31	405	6.30e-29	118
MS.gene042783.t1	AT4G03500	27.106	273	162	8	101	369	280	519	2.88e-18	87.8
MS.gene042783.t1	AT4G03500	27.106	273	162	8	101	369	280	519	2.88e-18	87.8
MS.gene042783.t1	AT4G03500	27.106	273	162	8	101	369	303	542	3.62e-18	87.4
MS.gene042783.t1	AT1G03670	27.402	281	164	8	107	382	282	527	1.93e-17	85.1
MS.gene042783.t1	AT2G01680	22.989	348	212	7	47	368	97	414	1.76e-16	82.0
MS.gene042783.t1	AT1G34050	24.691	324	201	8	60	369	166	460	2.05e-16	82.0
MS.gene042783.t1	AT4G03460	26.667	270	162	8	103	369	258	494	2.08e-16	82.0
MS.gene042783.t1	AT4G03460	27.306	271	159	9	103	369	290	526	2.47e-16	82.0
MS.gene042783.t1	AT5G54700	29.057	265	130	10	111	369	30	242	3.42e-16	80.1
MS.gene042783.t1	AT5G60070	22.246	463	304	15	2	443	102	529	1.35e-15	79.3
MS.gene042783.t1	AT3G12360	22.190	347	212	9	47	369	155	467	5.34e-15	77.8
MS.gene042783.t1	AT3G12360	22.254	346	211	9	47	368	167	478	6.86e-15	77.4
MS.gene042783.t1	AT1G07710	25.680	331	167	9	8	311	101	379	1.95e-14	75.9
MS.gene042783.t1	AT1G07710	25.680	331	167	9	8	311	101	379	1.95e-14	75.9
MS.gene042783.t1	AT1G10340	26.804	291	154	10	112	369	201	465	1.16e-13	73.6
MS.gene042783.t1	AT1G10340	26.804	291	154	10	112	369	205	469	1.20e-13	73.6
MS.gene042783.t1	AT4G03450	24.068	295	191	8	111	400	290	556	1.43e-13	73.2
MS.gene042783.t1	AT4G03450	22.475	396	234	11	12	400	227	556	1.55e-13	73.2
MS.gene042783.t1	AT5G54710	27.152	302	158	14	98	368	264	534	3.21e-13	72.0
MS.gene042783.t1	AT5G54710	26.061	330	177	15	98	391	219	517	3.21e-13	72.0
MS.gene042783.t1	AT3G09550	22.539	386	226	13	15	369	157	500	6.13e-13	71.2
MS.gene042783.t1	AT3G09550	24.316	329	197	11	15	312	150	457	6.30e-13	71.2
MS.gene042783.t1	AT3G09550	24.316	329	197	11	15	312	150	457	6.30e-13	71.2
MS.gene042783.t1	AT4G03490	26.875	320	160	14	8	312	207	467	1.73e-12	70.1
MS.gene042783.t1	AT4G03490	26.875	320	160	14	8	312	197	457	3.91e-12	68.6
MS.gene042783.t1	AT4G03490	26.875	320	160	14	8	312	207	467	4.52e-12	68.6
MS.gene042783.t1	AT1G05640	26.736	288	175	8	47	311	191	465	7.36e-12	67.8
MS.gene042783.t1	AT2G31820	24.927	341	202	11	47	362	227	538	9.32e-12	67.8

Find 87 sgRNAs with CRISPR-Local

Find 134 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTTACATGTTGCTGCATCTT+TGG	0.164398	6.1:+61413867	MS.gene042783:CDS
AGAGACCTTGTTGGAGTTAA+AGG	0.173222	6.1:+61414030	MS.gene042783:CDS
TGTCTAATGCGGTTTGATTC+TGG	0.239173	6.1:-61414929	None:intergenic
TATTATTATGACCCCAAGTT+TGG	0.253161	6.1:+61415358	MS.gene042783:CDS
CCTGAGAATATCTACATCTT+TGG	0.273051	6.1:-61415027	None:intergenic
TGTTATAGTTGATATGAAAA+AGG	0.273223	6.1:-61415333	None:intergenic
CTAACCATAGTTGTAATCTT+TGG	0.279193	6.1:-61415050	None:intergenic
CAATCGATTAAAACCATATA+TGG	0.299495	6.1:-61413740	None:intergenic
TTACATGTTGCTGCATCTTT+GGG	0.308483	6.1:+61413868	MS.gene042783:CDS
TGGGGTGGAGATAGTAGAAT+TGG	0.313441	6.1:-61415243	None:intergenic
ATGAACACAAATGCCATATA+TGG	0.331841	6.1:+61413727	MS.gene042783:CDS
CAGGCCAAAGATTACAACTA+TGG	0.355127	6.1:+61415046	MS.gene042783:CDS
TAGCGCTGAAATATGCAAAA+TGG	0.356278	6.1:-61414307	None:intergenic
AGCGATACACCATACGCTTT+TGG	0.356876	6.1:-61413993	None:intergenic
TTGCGCACATGCTCACATAT+AGG	0.371550	6.1:-61415542	None:intergenic
TCATAATTTCATTGGCAAAC+GGG	0.395107	6.1:-61413897	None:intergenic
TGCGCACATGCTCACATATA+GGG	0.401383	6.1:-61415541	None:intergenic
CTGAGAATATCTACATCTTT+GGG	0.405261	6.1:-61415026	None:intergenic
GTCAAGTATTCAATGGATTC+CGG	0.405345	6.1:-61414132	None:intergenic
TTCATAATTTCATTGGCAAA+CGG	0.405466	6.1:-61413898	None:intergenic
TATGACCCCAAGTTTGGGTC+AGG	0.409556	6.1:+61415364	MS.gene042783:CDS
TCTATACGCTGTAAAATGTA+TGG	0.410502	6.1:-61413820	None:intergenic
ATTATTATGACCCCAAGTTT+GGG	0.418551	6.1:+61415359	MS.gene042783:CDS
GAATGGCATATTAAACCATA+TGG	0.426035	6.1:+61414269	MS.gene042783:CDS
CTTCTATTGTTGGTAAGTAC+TGG	0.432315	6.1:+61414882	MS.gene042783:CDS
TGTTGCCGGCCTCATCCATA+TGG	0.432742	6.1:-61414284	None:intergenic
AGAATGCCCTGACCCAAACT+TGG	0.433975	6.1:-61415371	None:intergenic
CCCTTCAAGTACTTGTTGGA+TGG	0.443104	6.1:+61414208	MS.gene042783:CDS
ATTTAAAATCATCATTTGCT+TGG	0.443333	6.1:+61413920	MS.gene042783:CDS
GAGGCCCTTCAAGTACTTGT+TGG	0.447990	6.1:+61414204	MS.gene042783:CDS
ATCACAGGCACTTCTATTGT+TGG	0.458651	6.1:+61414872	MS.gene042783:intron
GGTGCTGAAGGGGTGGAGAA+TGG	0.460867	6.1:+61414252	MS.gene042783:CDS
TGTAAAGGAGTTTCAACAAA+TGG	0.467331	6.1:-61413850	None:intergenic
AATATGCAAAATGGTGTTGC+CGG	0.476164	6.1:-61414298	None:intergenic
GCCATCCAACAAGTACTTGA+AGG	0.497429	6.1:-61414209	None:intergenic
CCAAAGATGTAGATATTCTC+AGG	0.497521	6.1:+61415027	MS.gene042783:CDS
AGAAATCATTGAAACAATAG+TGG	0.506339	6.1:-61415471	None:intergenic
CCTCGTAGTCAAGTATTCAA+TGG	0.507786	6.1:-61414139	None:intergenic
AATACATTGCGAGGTGCTGA+AGG	0.511096	6.1:+61414240	MS.gene042783:CDS
GAATGCCCTGACCCAAACTT+GGG	0.512692	6.1:-61415370	None:intergenic
CTAATCTGATAAAGTCCACC+AGG	0.518575	6.1:-61415188	None:intergenic
AAGTACTGGTATAAATTTGA+AGG	0.522459	6.1:+61414896	MS.gene042783:CDS
AAAGATGCAGCAACATGTAA+AGG	0.527485	6.1:-61413865	None:intergenic
GGTGGAGATAGTAGAATTGG+AGG	0.529413	6.1:-61415240	None:intergenic
AAGAGTATATTATTCAGTGC+TGG	0.529470	6.1:+61414972	MS.gene042783:CDS
ATCTGACTTGCAAAATGAAG+AGG	0.535587	6.1:-61414069	None:intergenic
CGGAGAAGCAACGCAACACT+TGG	0.536689	6.1:+61415111	MS.gene042783:CDS
ATAGTGGTGTTGATATCTGC+GGG	0.536693	6.1:-61415455	None:intergenic
GTTGGAGTTAAAGGAAGAGA+AGG	0.552860	6.1:+61414039	MS.gene042783:CDS
CTCTTCCTTTAACTCCAACA+AGG	0.552979	6.1:-61414035	None:intergenic
GGACTTTATCAGATTAGTGC+TGG	0.556333	6.1:+61415194	MS.gene042783:CDS
AGCATCAATAGAGACCTTGT+TGG	0.559108	6.1:+61414021	MS.gene042783:CDS
TTTGTATAGCAAGATGGATG+GGG	0.562672	6.1:-61413966	None:intergenic
TAATAGCACCACAAAACAGC+GGG	0.568584	6.1:-61415511	None:intergenic
ACAAAATGACCAAAAGCGTA+TGG	0.576723	6.1:+61413984	MS.gene042783:CDS
ATACATTGCGAGGTGCTGAA+GGG	0.582154	6.1:+61414241	MS.gene042783:CDS
CATATTAAACCATATGGATG+AGG	0.582531	6.1:+61414275	MS.gene042783:CDS
TCATGGAAATCATATAACAT+AGG	0.585084	6.1:-61415421	None:intergenic
AGCGCTAAGTAGTGAGCCGC+AGG	0.585174	6.1:+61414323	MS.gene042783:CDS
ATTGCGAGGTGCTGAAGGGG+TGG	0.591371	6.1:+61414245	MS.gene042783:CDS
TTAAACCATATGGATGAGGC+CGG	0.591920	6.1:+61414279	MS.gene042783:CDS
TTGAATGCTGCAGCTCATGC+CGG	0.593816	6.1:+61413760	MS.gene042783:CDS
GCATCTGCGACTTCTATACC+TGG	0.598624	6.1:-61415002	None:intergenic
ATGACCCCAAGTTTGGGTCA+GGG	0.604365	6.1:+61415365	MS.gene042783:CDS
TTCAGTGCTGGATCAAAACC+AGG	0.605047	6.1:+61414984	MS.gene042783:CDS
TACATTGCGAGGTGCTGAAG+GGG	0.605652	6.1:+61414242	MS.gene042783:CDS
CATCTTGAATTACCGTGTAG+AGG	0.606191	6.1:-61413795	None:intergenic
CCATCCAACAAGTACTTGAA+GGG	0.609092	6.1:-61414208	None:intergenic
GCTTGGAAGTTAAATCCGCA+AGG	0.609839	6.1:+61413937	MS.gene042783:CDS
TAATTTCATTGGCAAACGGG+AGG	0.616124	6.1:-61413894	None:intergenic
AGTCAGAGCTGATTCATACA+TGG	0.618233	6.1:-61415162	None:intergenic
TATATGTGAGCATGTGCGCA+AGG	0.619775	6.1:+61415544	MS.gene042783:CDS
CTTGAAAATAATACATTGCG+AGG	0.623910	6.1:+61414231	MS.gene042783:CDS
GTAGAGGAGATCTACATCAC+CGG	0.624277	6.1:-61413779	None:intergenic
ACGTACTAGAGTTAATATAA+CGG	0.627553	6.1:+61415091	MS.gene042783:CDS
AATAGTGGTGTTGATATCTG+CGG	0.640850	6.1:-61415456	None:intergenic
AGTTGTTGCTATGTCTAATG+CGG	0.643349	6.1:-61414940	None:intergenic
AATGCCCTGACCCAAACTTG+GGG	0.643450	6.1:-61415369	None:intergenic
TGGATGGGGCTGAATCCTTG+CGG	0.670822	6.1:-61413952	None:intergenic
AGTGGTGTTGATATCTGCGG+GGG	0.682437	6.1:-61415453	None:intergenic
CCATTGAATACTTGACTACG+AGG	0.684610	6.1:+61414139	MS.gene042783:CDS
TGATGTAGATCTCCTCTACA+CGG	0.695151	6.1:+61413783	MS.gene042783:CDS
TAATCTGATAAAGTCCACCA+GGG	0.696202	6.1:-61415187	None:intergenic
TAGTGGTGTTGATATCTGCG+GGG	0.703629	6.1:-61415454	None:intergenic
ATAATAGCACCACAAAACAG+CGG	0.712605	6.1:-61415512	None:intergenic
AATCTGATAAAGTCCACCAG+GGG	0.723567	6.1:-61415186	None:intergenic
ATCTGATAAAGTCCACCAGG+GGG	0.726364	6.1:-61415185	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TCATAAAAAATTATCATTTA+AGG	-	chr6.1:61414522-61414541	None:intergenic	10.0%
!!!	ACATTTTAAATTAAAGTATA+TGG	+	chr6.1:61414660-61414679	MS.gene042783:intron	10.0%
!!!	TTTTAAATTCATAATTTCAT+TGG	-	chr6.1:61413908-61413927	None:intergenic	10.0%
!!	CCAAATATTAATGAATAATA+CGG	-	chr6.1:61414851-61414870	None:intergenic	15.0%
!!	TATTAAAAATCAACAATATG+AGG	+	chr6.1:61414185-61414204	MS.gene042783:CDS	15.0%
!!!	ATGATGATCATATTTTATAA+AGG	+	chr6.1:61414464-61414483	MS.gene042783:intron	15.0%
!!	ATAGAACACCAAATAAATAA+GGG	-	chr6.1:61414744-61414763	None:intergenic	20.0%
!!	CCGTATTATTCATTAATATT+TGG	+	chr6.1:61414848-61414867	MS.gene042783:intron	20.0%
!!!	ATTTAAAATCATCATTTGCT+TGG	+	chr6.1:61413920-61413939	MS.gene042783:CDS	20.0%
!!!	TGACATGTTATTTTAGTTAT+TGG	-	chr6.1:61414358-61414377	None:intergenic	20.0%
!!!	TGTTATAGTTGATATGAAAA+AGG	-	chr6.1:61415336-61415355	None:intergenic	20.0%
!	ACAATAAAAAAGAACTCACT+AGG	+	chr6.1:61414403-61414422	MS.gene042783:intron	25.0%
!	ACGTACTAGAGTTAATATAA+CGG	+	chr6.1:61415091-61415110	MS.gene042783:CDS	25.0%
!	AGAAATCATTGAAACAATAG+TGG	-	chr6.1:61415474-61415493	None:intergenic	25.0%
!	AGACAACGTATTAATTAGAT+AGG	+	chr6.1:61414791-61414810	MS.gene042783:intron	25.0%
!	ATGAATAATACGGTTGTTAA+AGG	-	chr6.1:61414841-61414860	None:intergenic	25.0%
!	ATGCTTAAATGAGTATTCTT+TGG	+	chr6.1:61414713-61414732	MS.gene042783:intron	25.0%
!	CAATCGATTAAAACCATATA+TGG	-	chr6.1:61413743-61413762	None:intergenic	25.0%
!	CATAGAACACCAAATAAATA+AGG	-	chr6.1:61414745-61414764	None:intergenic	25.0%
!	TCATGGAAATCATATAACAT+AGG	-	chr6.1:61415424-61415443	None:intergenic	25.0%
!	TCATTAATATTTGGTATCAC+AGG	+	chr6.1:61414857-61414876	MS.gene042783:intron	25.0%
!	TTCATAATTTCATTGGCAAA+CGG	-	chr6.1:61413901-61413920	None:intergenic	25.0%
!!!	AAGTACTGGTATAAATTTGA+AGG	+	chr6.1:61414896-61414915	MS.gene042783:CDS	25.0%
!!!	AGTGAGTTCTTTTTTATTGT+GGG	-	chr6.1:61414403-61414422	None:intergenic	25.0%
!!!	TAGTGAGTTCTTTTTTATTG+TGG	-	chr6.1:61414404-61414423	None:intergenic	25.0%
	ATGAACACAAATGCCATATA+TGG	+	chr6.1:61413727-61413746	MS.gene042783:CDS	30.0%
	ATTATTATGACCCCAAGTTT+GGG	+	chr6.1:61415359-61415378	MS.gene042783:CDS	30.0%
	CAACGTATTAATTAGATAGG+TGG	+	chr6.1:61414794-61414813	MS.gene042783:intron	30.0%
	CATATTAAACCATATGGATG+AGG	+	chr6.1:61414275-61414294	MS.gene042783:CDS	30.0%
	CTAACCATAGTTGTAATCTT+TGG	-	chr6.1:61415053-61415072	None:intergenic	30.0%
	CTGAGAATATCTACATCTTT+GGG	-	chr6.1:61415029-61415048	None:intergenic	30.0%
	CTTGAAAATAATACATTGCG+AGG	+	chr6.1:61414231-61414250	MS.gene042783:CDS	30.0%
	GAATGGCATATTAAACCATA+TGG	+	chr6.1:61414269-61414288	MS.gene042783:CDS	30.0%
	TATTATTATGACCCCAAGTT+TGG	+	chr6.1:61415358-61415377	MS.gene042783:CDS	30.0%
	TCATAATTTCATTGGCAAAC+GGG	-	chr6.1:61413900-61413919	None:intergenic	30.0%
	TCTATACGCTGTAAAATGTA+TGG	-	chr6.1:61413823-61413842	None:intergenic	30.0%
	TGTAAAGGAGTTTCAACAAA+TGG	-	chr6.1:61413853-61413872	None:intergenic	30.0%
	TTATTGGATAACATTACCTG+CGG	-	chr6.1:61414342-61414361	None:intergenic	30.0%
!	AAGAGTATATTATTCAGTGC+TGG	+	chr6.1:61414972-61414991	MS.gene042783:CDS	30.0%
!	ATTTTGTATAGCAAGATGGA+TGG	-	chr6.1:61413971-61413990	None:intergenic	30.0%
!	TGGCAAAACCCTTATTTATT+TGG	+	chr6.1:61414733-61414752	MS.gene042783:intron	30.0%
!	TTACTTTCTATGCTTTTGTC+CGG	+	chr6.1:61414113-61414132	MS.gene042783:CDS	30.0%
!	TTTTGTATAGCAAGATGGAT+GGG	-	chr6.1:61413970-61413989	None:intergenic	30.0%
!!!	ACAGATTTCCCAACATTTTT+TGG	-	chr6.1:61415266-61415285	None:intergenic	30.0%
!!!	AGATTTCCCAACATTTTTTG+GGG	-	chr6.1:61415264-61415283	None:intergenic	30.0%
!!!	CAGATTTCCCAACATTTTTT+GGG	-	chr6.1:61415265-61415284	None:intergenic	30.0%
!!!	GTGAGTTCTTTTTTATTGTG+GGG	-	chr6.1:61414402-61414421	None:intergenic	30.0%
	AAAGATGCAGCAACATGTAA+AGG	-	chr6.1:61413868-61413887	None:intergenic	35.0%
	AGGAAGAAAAAACACATTGC+AGG	-	chr6.1:61415404-61415423	None:intergenic	35.0%
	AGTTGTTGCTATGTCTAATG+CGG	-	chr6.1:61414943-61414962	None:intergenic	35.0%
	ATAATAGCACCACAAAACAG+CGG	-	chr6.1:61415515-61415534	None:intergenic	35.0%
	CCAAAGATGTAGATATTCTC+AGG	+	chr6.1:61415027-61415046	MS.gene042783:CDS	35.0%
	CCTGAGAATATCTACATCTT+TGG	-	chr6.1:61415030-61415049	None:intergenic	35.0%
	GGAACCTGAAAGAAAATGAT+TGG	+	chr6.1:61414424-61414443	MS.gene042783:intron	35.0%
	GGAAGAAAAAACACATTGCA+GGG	-	chr6.1:61415403-61415422	None:intergenic	35.0%
	TAATCTGATAAAGTCCACCA+GGG	-	chr6.1:61415190-61415209	None:intergenic	35.0%
	TTAAGGTCCTTGAAGAGTAA+CGG	-	chr6.1:61414505-61414524	None:intergenic	35.0%
	TTACATGTTGCTGCATCTTT+GGG	+	chr6.1:61413868-61413887	MS.gene042783:CDS	35.0%
	TTTACATGTTGCTGCATCTT+TGG	+	chr6.1:61413867-61413886	MS.gene042783:CDS	35.0%
	TTTGTATAGCAAGATGGATG+GGG	-	chr6.1:61413969-61413988	None:intergenic	35.0%
!	ACAAAATGACCAAAAGCGTA+TGG	+	chr6.1:61413984-61414003	MS.gene042783:CDS	35.0%
!	ATCTGACTTGCAAAATGAAG+AGG	-	chr6.1:61414072-61414091	None:intergenic	35.0%
!	CATGCCAATCATTTTCTTTC+AGG	-	chr6.1:61414431-61414450	None:intergenic	35.0%
!	CTTCATTTTGCAAGTCAGAT+AGG	+	chr6.1:61414072-61414091	MS.gene042783:CDS	35.0%
!	CTTCTATTGTTGGTAAGTAC+TGG	+	chr6.1:61414882-61414901	MS.gene042783:CDS	35.0%
!	GTCAAGTATTCAATGGATTC+CGG	-	chr6.1:61414135-61414154	None:intergenic	35.0%
!	GTTGTCTTTCGTGTTTCTAT+AGG	-	chr6.1:61414778-61414797	None:intergenic	35.0%
!	TAGCGCTGAAATATGCAAAA+TGG	-	chr6.1:61414310-61414329	None:intergenic	35.0%
!!	AATAGTGGTGTTGATATCTG+CGG	-	chr6.1:61415459-61415478	None:intergenic	35.0%
!!	AATATGCAAAATGGTGTTGC+CGG	-	chr6.1:61414301-61414320	None:intergenic	35.0%
!!	GGTCATTTTGTATAGCAAGA+TGG	-	chr6.1:61413975-61413994	None:intergenic	35.0%
	AATCTGATAAAGTCCACCAG+GGG	-	chr6.1:61415189-61415208	None:intergenic	40.0%
	AGAGACCTTGTTGGAGTTAA+AGG	+	chr6.1:61414030-61414049	MS.gene042783:CDS	40.0%
	AGCATCAATAGAGACCTTGT+TGG	+	chr6.1:61414021-61414040	MS.gene042783:CDS	40.0%
	ATCACAGGCACTTCTATTGT+TGG	+	chr6.1:61414872-61414891	MS.gene042783:intron	40.0%
	ATCTCCACCCCAAAAAATGT+TGG	+	chr6.1:61415254-61415273	MS.gene042783:CDS	40.0%
	CAGGCCAAAGATTACAACTA+TGG	+	chr6.1:61415046-61415065	MS.gene042783:CDS	40.0%
	CATCTTGAATTACCGTGTAG+AGG	-	chr6.1:61413798-61413817	None:intergenic	40.0%
	CCATCCAACAAGTACTTGAA+GGG	-	chr6.1:61414211-61414230	None:intergenic	40.0%
	CCATTGAATACTTGACTACG+AGG	+	chr6.1:61414139-61414158	MS.gene042783:CDS	40.0%
	CCTCGTAGTCAAGTATTCAA+TGG	-	chr6.1:61414142-61414161	None:intergenic	40.0%
	CTAATCTGATAAAGTCCACC+AGG	-	chr6.1:61415191-61415210	None:intergenic	40.0%
	CTCTTCCTTTAACTCCAACA+AGG	-	chr6.1:61414038-61414057	None:intergenic	40.0%
	GGACTTTATCAGATTAGTGC+TGG	+	chr6.1:61415194-61415213	MS.gene042783:CDS	40.0%
	GTTGGAGTTAAAGGAAGAGA+AGG	+	chr6.1:61414039-61414058	MS.gene042783:CDS	40.0%
	TAATAGCACCACAAAACAGC+GGG	-	chr6.1:61415514-61415533	None:intergenic	40.0%
	TAATTTCATTGGCAAACGGG+AGG	-	chr6.1:61413897-61413916	None:intergenic	40.0%
	TCTCCACCCCAAAAAATGTT+GGG	+	chr6.1:61415255-61415274	MS.gene042783:CDS	40.0%
	TGATGTAGATCTCCTCTACA+CGG	+	chr6.1:61413783-61413802	MS.gene042783:CDS	40.0%
	TGTCTAATGCGGTTTGATTC+TGG	-	chr6.1:61414932-61414951	None:intergenic	40.0%
	TTAAACCATATGGATGAGGC+CGG	+	chr6.1:61414279-61414298	MS.gene042783:CDS	40.0%
!	AGTCAGAGCTGATTCATACA+TGG	-	chr6.1:61415165-61415184	None:intergenic	40.0%
!!	ATAGTGGTGTTGATATCTGC+GGG	-	chr6.1:61415458-61415477	None:intergenic	40.0%
!!	TTATGTTTCCCGCTGTTTTG+TGG	+	chr6.1:61415503-61415522	MS.gene042783:CDS	40.0%
!!!	TTTCCCAACATTTTTTGGGG+TGG	-	chr6.1:61415261-61415280	None:intergenic	40.0%
	ACGAATGCCGTTACTCTTCA+AGG	+	chr6.1:61414495-61414514	MS.gene042783:intron	45.0%
	ATCTGATAAAGTCCACCAGG+GGG	-	chr6.1:61415188-61415207	None:intergenic	45.0%
	GCCATCCAACAAGTACTTGA+AGG	-	chr6.1:61414212-61414231	None:intergenic	45.0%
	GCTTGGAAGTTAAATCCGCA+AGG	+	chr6.1:61413937-61413956	MS.gene042783:CDS	45.0%
	GGTGGAGATAGTAGAATTGG+AGG	-	chr6.1:61415243-61415262	None:intergenic	45.0%
	GTAGAGGAGATCTACATCAC+CGG	-	chr6.1:61413782-61413801	None:intergenic	45.0%
	TATATGTGAGCATGTGCGCA+AGG	+	chr6.1:61415544-61415563	MS.gene042783:CDS	45.0%
	TGCGCACATGCTCACATATA+GGG	-	chr6.1:61415544-61415563	None:intergenic	45.0%
	TGGGGTGGAGATAGTAGAAT+TGG	-	chr6.1:61415246-61415265	None:intergenic	45.0%
	TTGCGCACATGCTCACATAT+AGG	-	chr6.1:61415545-61415564	None:intergenic	45.0%
!	AGCGATACACCATACGCTTT+TGG	-	chr6.1:61413996-61414015	None:intergenic	45.0%
!	CCCTTCAAGTACTTGTTGGA+TGG	+	chr6.1:61414208-61414227	MS.gene042783:CDS	45.0%
!	TTCAGTGCTGGATCAAAACC+AGG	+	chr6.1:61414984-61415003	MS.gene042783:CDS	45.0%
!!	AATACATTGCGAGGTGCTGA+AGG	+	chr6.1:61414240-61414259	MS.gene042783:CDS	45.0%
!!	ATACATTGCGAGGTGCTGAA+GGG	+	chr6.1:61414241-61414260	MS.gene042783:CDS	45.0%
!!	TAGTGGTGTTGATATCTGCG+GGG	-	chr6.1:61415457-61415476	None:intergenic	45.0%
!!!	TAATGAATAATTTTTTAATT+TGG	+	chr6.1:61414689-61414708	MS.gene042783:intron	5.0%
	AATGCCCTGACCCAAACTTG+GGG	-	chr6.1:61415372-61415391	None:intergenic	50.0%
	ACAAATGCCAACGAGTGCTC+TGG	-	chr6.1:61414631-61414650	None:intergenic	50.0%
	AGAATGCCCTGACCCAAACT+TGG	-	chr6.1:61415374-61415393	None:intergenic	50.0%
	ATGACCCCAAGTTTGGGTCA+GGG	+	chr6.1:61415365-61415384	MS.gene042783:CDS	50.0%
	CAAATGCCAACGAGTGCTCT+GGG	-	chr6.1:61414630-61414649	None:intergenic	50.0%
	GAATGCCCTGACCCAAACTT+GGG	-	chr6.1:61415373-61415392	None:intergenic	50.0%
	GAGGCCCTTCAAGTACTTGT+TGG	+	chr6.1:61414204-61414223	MS.gene042783:CDS	50.0%
	GCATCTGCGACTTCTATACC+TGG	-	chr6.1:61415005-61415024	None:intergenic	50.0%
	TATGACCCCAAGTTTGGGTC+AGG	+	chr6.1:61415364-61415383	MS.gene042783:CDS	50.0%
	TTGAATGCTGCAGCTCATGC+CGG	+	chr6.1:61413760-61413779	MS.gene042783:CDS	50.0%
!!	AGTGGTGTTGATATCTGCGG+GGG	-	chr6.1:61415456-61415475	None:intergenic	50.0%
!!	TACATTGCGAGGTGCTGAAG+GGG	+	chr6.1:61414242-61414261	MS.gene042783:CDS	50.0%
	CGGAGAAGCAACGCAACACT+TGG	+	chr6.1:61415111-61415130	MS.gene042783:CDS	55.0%
	TGCGGGGGATATCTCACTCA+TGG	-	chr6.1:61415441-61415460	None:intergenic	55.0%
	TGGATGGGGCTGAATCCTTG+CGG	-	chr6.1:61413955-61413974	None:intergenic	55.0%
	TGTTGCCGGCCTCATCCATA+TGG	-	chr6.1:61414287-61414306	None:intergenic	55.0%
!	GAGTATCCCAGAGCACTCGT+TGG	+	chr6.1:61414621-61414640	MS.gene042783:intron	55.0%
	AGCGCTAAGTAGTGAGCCGC+AGG	+	chr6.1:61414323-61414342	MS.gene042783:CDS	60.0%
!	GAATCAGCTCTGACTCCCCC+TGG	+	chr6.1:61415170-61415189	MS.gene042783:CDS	60.0%
!	GGTGCTGAAGGGGTGGAGAA+TGG	+	chr6.1:61414252-61414271	MS.gene042783:CDS	60.0%
!!	ATTGCGAGGTGCTGAAGGGG+TGG	+	chr6.1:61414245-61414264	MS.gene042783:CDS	60.0%
!	TCAGCTCTGACTCCCCCTGG+TGG	+	chr6.1:61415173-61415192	MS.gene042783:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.1	gene	61413727	61415592	61413727	ID=MS.gene042783
chr6.1	mRNA	61413727	61415592	61413727	ID=MS.gene042783.t1;Parent=MS.gene042783
chr6.1	exon	61413727	61414344	61413727	ID=MS.gene042783.t1.exon1;Parent=MS.gene042783.t1
chr6.1	CDS	61413727	61414344	61413727	ID=cds.MS.gene042783.t1;Parent=MS.gene042783.t1
chr6.1	exon	61414879	61415592	61414879	ID=MS.gene042783.t1.exon2;Parent=MS.gene042783.t1
chr6.1	CDS	61414879	61415592	61414879	ID=cds.MS.gene042783.t1;Parent=MS.gene042783.t1

Gene Sequence

>MS.gene042783

ATGAACACAAATGCCATATATGGTTTTAATCGATTGAATGCTGCAGCTCATGCCGGTGATGTAGATCTCCTCTACACGGTAATTCAAGATGATCCATACATTTTACAGCGTATAGATTTGATACCATTTGTTGAAACTCCTTTACATGTTGCTGCATCTTTGGGACACCTCCCGTTTGCCAATGAAATTATGAATTTAAAATCATCATTTGCTTGGAAGTTAAATCCGCAAGGATTCAGCCCCATCCATCTTGCTATACAAAATGACCAAAAGCGTATGGTGTATCGCTTTGTAAGCATCAATAGAGACCTTGTTGGAGTTAAAGGAAGAGAAGGCTTGACTCCTCTTCATTTTGCAAGTCAGATAGGAGAAGTTGATCTTTTATATTACTTTCTATGCTTTTGTCCGGAATCCATTGAATACTTGACTACGAGGCACGAGACTGCACTGCATATTGCTATTAAAAATCAACAATATGAGGCCCTTCAAGTACTTGTTGGATGGCTTGAAAATAATACATTGCGAGGTGCTGAAGGGGTGGAGAATGGCATATTAAACCATATGGATGAGGCCGGCAACACCATTTTGCATATTTCAGCGCTAAGTAGTGAGCCGCAGGTAATGTTATCCAATAACTAAAATAACATGTCATCTTTATATATTTCTTGTTTTCCCCACAATAAAAAAGAACTCACTAGGAACCTGAAAGAAAATGATTGGCATGTGTTTTGCTGTAAATGATGATCATATTTTATAAAGGAAATGCTAACGAATGCCGTTACTCTTCAAGGACCTTAAATGATAATTTTTTATGAAAATTTGTGTGTTCAATGCATTTGAAATTAAAGTATATGATTTTGTTAATAAATAATTTTTTAATTTAGTATGCTTAAAGAGTATCCCAGAGCACTCGTTGGCATTTGTGTATTCAATACATTTTAAATTAAAGTATATGGTTTTTTTAATGAATAATTTTTTAATTTGGTATGCTTAAATGAGTATTCTTTGGCAAAACCCTTATTTATTTGGTGTTCTATGTCTTCTTTTTCCTATAGAAACACGAAAGACAACGTATTAATTAGATAGGTGGCATAGTTGCTAACATGAATGACCTTTAACAACCGTATTATTCATTAATATTTGGTATCACAGGCACTTCTATTGTTGGTAAGTACTGGTATAAATTTGAAGGCAAAGAATTTCCAGAATCAAACCGCATTAGACATAGCAACAACTGCAGAGATGAAGAGTATATTATTCAGTGCTGGATCAAAACCAGGTATAGAAGTCGCAGATGCTCCCAAAGATGTAGATATTCTCAGGCCAAAGATTACAACTATGGTTAGATTTTCAATTCGTACATTACGTACTAGAGTTAATATAACGGAGAAGCAACGCAACACTTGGTTAATAGTTGCGACTCTTATTGCAACTGCCATGTATGAATCAGCTCTGACTCCCCCTGGTGGACTTTATCAGATTAGTGCTGGTGACAATAATTTGAACATCACTTCCTCCAATTCTACTATCTCCACCCCAAAAAATGTTGGGAAATCTGTATTGTCAAAACTTGACTTCATGCTGTTTTCAGTTTTGAATATGATTTCCTTTTTCATATCAACTATAACAATTATTATTATGACCCCAAGTTTGGGTCAGGGCATTCTTGTGTACCCTGCAATGTGTTTTTTCTTCCTATGTTATATGATTTCCATGAGTGAGATATCCCCCGCAGATATCAACACCACTATTGTTTCAATGATTTCTATTTTAACTTTATGTTTCCCGCTGTTTTGTGGTGCTATTATAATGTACCCTATATGTGAGCATGTGCGCAAGGCTAAGAAGCTGATGAGTAGATCATAG

Protein sequence

>MS.gene042783.t1

MNTNAIYGFNRLNAAAHAGDVDLLYTVIQDDPYILQRIDLIPFVETPLHVAASLGHLPFANEIMNLKSSFAWKLNPQGFSPIHLAIQNDQKRMVYRFVSINRDLVGVKGREGLTPLHFASQIGEVDLLYYFLCFCPESIEYLTTRHETALHIAIKNQQYEALQVLVGWLENNTLRGAEGVENGILNHMDEAGNTILHISALSSEPQALLLLVSTGINLKAKNFQNQTALDIATTAEMKSILFSAGSKPGIEVADAPKDVDILRPKITTMVRFSIRTLRTRVNITEKQRNTWLIVATLIATAMYESALTPPGGLYQISAGDNNLNITSSNSTISTPKNVGKSVLSKLDFMLFSVLNMISFFISTITIIIMTPSLGQGILVYPAMCFFFLCYMISMSEISPADINTTIVSMISILTLCFPLFCGAIIMYPICEHVRKAKKLMSRS