AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene071113

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene071113.t1 MTR_8g072510 76.577 333 77 1 2 334 10 341 0.0 510
MS.gene071113.t1 MTR_8g039620 70.605 347 90 3 2 337 14 359 2.98e-170 481
MS.gene071113.t1 MTR_7g021090 71.471 333 89 1 2 334 9 335 1.72e-168 476
MS.gene071113.t1 MTR_8g062410 73.353 334 81 2 2 334 10 336 2.55e-164 467
MS.gene071113.t1 MTR_1g059930 68.468 333 105 0 2 334 12 344 4.55e-161 458
MS.gene071113.t1 MTR_3g016210 65.341 352 106 3 2 338 10 360 3.61e-156 446
MS.gene071113.t1 MTR_8g068990 64.058 345 102 5 2 341 12 339 7.23e-148 425
MS.gene071113.t1 MTR_7g451420 67.763 304 98 0 2 305 9 312 9.63e-144 409
MS.gene071113.t1 MTR_8g073260 61.677 334 121 2 2 333 11 339 1.09e-143 414
MS.gene071113.t1 MTR_8g069880 62.575 334 122 3 1 333 6 337 4.91e-136 395
MS.gene071113.t1 MTR_8g040930 65.188 293 98 2 44 333 1 292 2.68e-131 380
MS.gene071113.t1 MTR_8g041240 65.188 293 98 2 44 333 1 292 2.68e-131 380
MS.gene071113.t1 MTR_8g467780 57.726 343 135 2 2 334 11 353 4.83e-126 370
MS.gene071113.t1 MTR_8g066670 61.377 334 126 3 2 334 1075 1406 4.64e-124 389
MS.gene071113.t1 MTR_3g105650 50.000 308 146 2 2 302 5 311 4.62e-99 300
MS.gene071113.t1 MTR_8g073275 43.454 359 125 8 44 335 1 348 4.52e-79 249
MS.gene071113.t1 MTR_1g069110 26.531 343 219 8 2 338 18 333 1.32e-25 107
MS.gene071113.t1 MTR_7g099580 26.214 309 177 8 2 305 12 274 4.62e-25 104
MS.gene071113.t1 MTR_2g101320 27.711 249 151 5 30 261 134 370 4.75e-21 94.7
MS.gene071113.t1 MTR_1g115575 28.155 309 167 9 37 314 169 453 1.53e-20 93.2
MS.gene071113.t1 MTR_7g099570 30.412 194 121 3 2 190 9 193 2.08e-19 90.1
MS.gene071113.t1 MTR_7g117620 26.384 307 192 6 37 321 119 413 2.25e-19 89.7
MS.gene071113.t1 MTR_2g096190 25.000 288 180 6 38 301 105 380 2.29e-17 83.6
MS.gene071113.t1 MTR_4g062100 25.347 288 179 6 38 301 109 384 1.32e-16 81.3
MS.gene071113.t1 MTR_2g101310 27.160 243 153 4 33 261 137 369 1.57e-16 80.9
MS.gene071113.t1 MTR_2g101310 27.160 243 153 4 33 261 137 369 2.02e-16 80.9
MS.gene071113.t1 MTR_6g027840 29.592 196 126 6 37 223 246 438 3.54e-15 77.0
MS.gene071113.t1 MTR_2g033050 29.808 208 127 5 53 256 217 409 1.11e-14 75.1
MS.gene071113.t1 MTR_1g032100 24.483 290 182 7 37 301 94 371 1.14e-14 75.5
MS.gene071113.t1 MTR_5g085500 34.615 130 73 4 98 223 35 156 2.65e-14 74.3
MS.gene071113.t1 MTR_7g100390 24.675 308 195 5 37 320 87 381 4.93e-14 73.6
MS.gene071113.t1 MTR_5g085830 26.087 207 133 5 52 256 293 481 9.32e-14 72.8
MS.gene071113.t1 MTR_5g085480 26.425 193 96 5 66 220 240 424 1.95e-13 72.0
MS.gene071113.t1 MTR_5g085480 26.425 193 96 5 66 220 80 264 2.16e-13 71.6
MS.gene071113.t1 MTR_8g105260 23.103 290 186 5 37 301 101 378 2.54e-13 71.2
MS.gene071113.t1 MTR_7g100430 25.077 323 178 12 35 302 124 437 2.75e-13 71.2
MS.gene071113.t1 MTR_5g085810 27.184 206 130 5 53 256 320 507 2.41e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene071113.t1 AT4G10720 41.379 319 179 2 2 312 5 323 2.17e-78 246
MS.gene071113.t1 AT4G10720 41.379 319 179 2 2 312 5 323 5.21e-78 246
MS.gene071113.t1 AT5G15500 39.222 334 186 6 2 323 6 334 5.65e-73 234
MS.gene071113.t1 AT1G14500 37.700 313 186 2 2 305 5 317 1.79e-72 232
MS.gene071113.t1 AT1G14480 37.771 323 190 3 2 313 5 327 2.06e-70 226
MS.gene071113.t1 AT5G54620 39.597 298 173 2 9 299 12 309 1.13e-67 219
MS.gene071113.t1 AT5G54610 36.027 297 181 3 7 296 10 304 5.67e-60 199
MS.gene071113.t1 AT1G14480 36.624 314 163 5 2 313 5 284 7.36e-55 185
MS.gene071113.t1 AT5G15500 33.906 233 137 6 103 323 1 228 1.85e-32 124
MS.gene071113.t1 AT5G51160 31.022 274 161 5 56 308 35 301 3.82e-31 122
MS.gene071113.t1 AT1G34050 26.538 260 169 5 58 305 174 423 1.68e-19 90.1
MS.gene071113.t1 AT1G03670 31.020 245 120 9 81 308 266 478 3.86e-19 89.4
MS.gene071113.t1 AT4G03500 27.391 230 131 6 85 302 297 502 1.32e-17 84.7
MS.gene071113.t1 AT4G03500 27.391 230 131 6 85 302 274 479 1.60e-17 84.3
MS.gene071113.t1 AT4G03500 27.391 230 131 6 85 302 274 479 1.60e-17 84.3
MS.gene071113.t1 AT3G12360 26.384 307 175 8 37 314 155 439 6.25e-17 82.4
MS.gene071113.t1 AT3G12360 26.384 307 175 8 37 314 167 451 6.28e-17 82.4
MS.gene071113.t1 AT4G03460 30.045 223 117 6 93 302 290 486 1.30e-16 81.6
MS.gene071113.t1 AT4G03460 30.045 223 117 6 93 302 258 454 1.43e-16 81.3
MS.gene071113.t1 AT2G01680 25.000 300 174 6 37 304 97 377 6.01e-16 79.3
MS.gene071113.t1 AT1G10340 28.692 237 127 4 102 305 205 432 8.37e-16 79.0
MS.gene071113.t1 AT2G24600 25.743 303 181 7 61 328 163 456 9.95e-16 79.0
MS.gene071113.t1 AT2G24600 25.743 303 181 7 61 328 163 456 9.95e-16 79.0
MS.gene071113.t1 AT1G10340 29.958 237 124 5 102 305 201 428 1.03e-15 79.0
MS.gene071113.t1 AT2G24600 25.899 278 165 6 61 305 163 432 1.35e-15 78.2
MS.gene071113.t1 AT2G24600 25.899 278 165 6 61 305 163 432 1.35e-15 78.2
MS.gene071113.t1 AT1G07710 25.077 323 206 7 2 301 70 379 2.73e-15 77.4
MS.gene071113.t1 AT1G07710 25.077 323 206 7 2 301 70 379 2.73e-15 77.4
MS.gene071113.t1 AT1G05640 23.229 353 184 10 35 301 114 465 5.76e-15 76.6
MS.gene071113.t1 AT5G54700 27.313 227 125 6 88 309 17 208 6.14e-15 75.5
MS.gene071113.t1 AT3G09550 24.928 345 193 11 5 314 157 470 6.82e-15 76.3
MS.gene071113.t1 AT3G09550 24.928 345 193 11 5 314 150 463 6.89e-15 76.3
MS.gene071113.t1 AT3G09550 24.928 345 193 11 5 314 150 463 6.89e-15 76.3
MS.gene071113.t1 AT4G03450 26.214 206 137 4 101 302 290 484 1.72e-14 75.1
MS.gene071113.t1 AT4G03450 26.087 207 138 4 101 303 290 485 1.82e-14 75.1
MS.gene071113.t1 AT5G54710 27.801 241 137 8 91 305 222 451 3.80e-14 73.9
MS.gene071113.t1 AT5G54710 27.801 241 137 8 91 305 267 496 4.67e-14 73.9
MS.gene071113.t1 AT5G54700 24.746 295 151 8 17 309 20 245 3.12e-13 70.9
MS.gene071113.t1 AT5G60070 24.179 335 198 12 2 301 77 390 9.21e-13 69.7
MS.gene071113.t1 AT2G31820 26.531 245 148 7 24 253 208 435 1.19e-12 69.7
MS.gene071113.t1 AT5G50140 28.902 173 98 4 33 190 26 188 3.20e-12 68.2
MS.gene071113.t1 AT5G02620 23.368 291 184 6 38 301 95 373 5.98e-12 67.0
MS.gene071113.t1 AT5G02620 23.368 291 184 6 38 301 95 373 5.98e-12 67.0
MS.gene071113.t1 AT5G02620 23.368 291 184 6 38 301 95 373 5.98e-12 67.0

Find 77 sgRNAs with CRISPR-Local

Find 134 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAGACCTTGTTAGAGTTAA+AGG 0.117136 4.3:-51625786 MS.gene071113:CDS
GTTACATATTGCTGCATCTA+TGG 0.262404 4.3:-51625949 MS.gene071113:CDS
GGTAAGTACTGGTATGAATT+TGG 0.286305 4.3:-51625363 MS.gene071113:CDS
GGTTCTCAAAAGTTGGAAAA+TGG 0.307640 4.3:-51625564 MS.gene071113:CDS
CGGAGATGATCCATTCATTC+TGG 0.324894 4.3:-51626006 MS.gene071113:CDS
TTCATAATTTCAATGGTAAA+CGG 0.330193 4.3:+51625918 None:intergenic
TAGTGCCGAAATATGCAAAA+TGG 0.358761 4.3:+51625509 None:intergenic
TACTAGAGAGCCTAGTATTC+CGG 0.388628 4.3:+51624878 None:intergenic
CTGATTTCCCAGCATTTCCT+AGG 0.390015 4.3:+51624991 None:intergenic
TAGGGTAGAAATAGTAGAAT+TGG 0.390967 4.3:+51625010 None:intergenic
TCATAATTTCAATGGTAAAC+GGG 0.395809 4.3:+51625919 None:intergenic
GTTCACGCCGCTGTCATCAT+TGG 0.413619 4.3:+51625043 None:intergenic
ATTTAAAACCTTCATTTGCT+TGG 0.418134 4.3:-51625896 MS.gene071113:CDS
AATATGCAAAATGGTGTTGC+CGG 0.418241 4.3:+51625518 None:intergenic
GCAGCTGAAAGCTGCAGCTC+AGG 0.418481 4.3:-51626060 MS.gene071113:intron
GGAATTTCTCTTTCTTTGTC+CGG 0.421807 4.3:-51625703 MS.gene071113:CDS
CTTCTATTGTTGGTAAGTAC+TGG 0.432315 4.3:-51625374 MS.gene071113:CDS
ATGATACCAGCCGGAATACT+AGG 0.433021 4.3:-51624888 MS.gene071113:CDS
TCGAAGAGGTTCTCAAAAGT+TGG 0.435517 4.3:-51625571 MS.gene071113:CDS
GATCTCCTCTACGCAGTAAT+CGG 0.440555 4.3:-51626026 MS.gene071113:CDS
CCCTTCAAATACTTGTTAGC+TGG 0.445898 4.3:-51625608 MS.gene071113:CDS
TGTTGCCGGCTTTATCCCTC+TGG 0.449876 4.3:+51625532 None:intergenic
ATTTCTACCCTAGGAAATGC+TGG 0.455059 4.3:-51624999 MS.gene071113:CDS
TCTATATGCTCCAGAATGAA+TGG 0.459267 4.3:+51625996 None:intergenic
AGCACAGGCACTTCTATTGT+TGG 0.470486 4.3:-51625384 MS.gene071113:intron
GTCAAGTATTCAAGGGAATC+CGG 0.474006 4.3:+51625684 None:intergenic
CTCTCTAGTAGGTTCTCCCA+TGG 0.474964 4.3:-51624865 MS.gene071113:CDS
AATAATATACTCTTGATCTC+TGG 0.484204 4.3:+51625293 None:intergenic
GGCACTAAGTTGTGAGCCGC+AGG 0.488252 4.3:-51625493 MS.gene071113:intron
TGTAACGGAGTTTCAACAAA+TGG 0.492054 4.3:+51625966 None:intergenic
TTTAGCTTCCAAGCAAATGA+AGG 0.492400 4.3:+51625888 None:intergenic
TAGTAGGTTCTCCCATGGGT+TGG 0.493109 4.3:-51624860 MS.gene071113:CDS
GCCAGCTAACAAGTATTTGA+AGG 0.497511 4.3:+51625607 None:intergenic
TGCTTGGAAGCTAAATCCGC+AGG 0.508838 4.3:-51625880 MS.gene071113:CDS
TGTTAAGAATCAACAATATG+AGG 0.510561 4.3:-51625631 MS.gene071113:CDS
AATTCTACTATTTCTACCCT+AGG 0.517193 4.3:-51625008 MS.gene071113:CDS
ACCTCACAGTCAAGTATTCA+AGG 0.520683 4.3:+51625676 None:intergenic
GTTAGAGTTAAAGGAAGAGA+GGG 0.523986 4.3:-51625777 MS.gene071113:CDS
AAGAGTATATTATTAAGTGT+TGG 0.527914 4.3:-51625284 MS.gene071113:CDS
TTACATATTGCTGCATCTAT+GGG 0.532476 4.3:-51625948 MS.gene071113:CDS
CAGAGGAGCAACGCAACACT+TGG 0.546199 4.3:-51625145 MS.gene071113:CDS
GGAATACTAGGCTCTCTAGT+AGG 0.546860 4.3:-51624876 MS.gene071113:CDS
GCAACTGCGATCTATCAATC+CGG 0.547636 4.3:-51625101 MS.gene071113:CDS
GGTAGAAATAGTAGAATTGG+CGG 0.556035 4.3:+51625013 None:intergenic
GCACACAGGCACAAAGGCTC+AGG 0.556544 4.3:-51625229 MS.gene071113:CDS
CTGAAAGCTGCAGCTCAGGA+AGG 0.558773 4.3:-51626056 MS.gene071113:CDS
CTCTTCCTTTAACTCTAACA+AGG 0.564990 4.3:+51625781 None:intergenic
TGTTAGAGTTAAAGGAAGAG+AGG 0.566339 4.3:-51625778 MS.gene071113:CDS
TCTCTAGTAGGTTCTCCCAT+GGG 0.580455 4.3:-51624864 MS.gene071113:CDS
AAATGGAATATTAAACCAGA+GGG 0.585975 4.3:-51625547 MS.gene071113:CDS
AGAGAGCCTAGTATTCCGGC+TGG 0.587717 4.3:+51624882 None:intergenic
GAATTTGGACGCAAAGAATG+AGG 0.588412 4.3:-51625348 MS.gene071113:CDS
TTTCTACCCTAGGAAATGCT+GGG 0.590604 4.3:-51624998 MS.gene071113:CDS
TGATTTCCCAGCATTTCCTA+GGG 0.591052 4.3:+51624992 None:intergenic
CTAACCTGATAAATTCCACC+AGG 0.592230 4.3:+51625068 None:intergenic
ATATATGTTATGATACCAGC+CGG 0.593181 4.3:-51624897 MS.gene071113:CDS
CCAGCTAACAAGTATTTGAA+GGG 0.614265 4.3:+51625608 None:intergenic
CCCTTGAATACTTGACTGTG+AGG 0.615568 4.3:-51625677 MS.gene071113:CDS
CTCAAACGAAATAATCGAAG+AGG 0.617825 4.3:-51625585 MS.gene071113:CDS
GAATCAGTGACTTCTAAGCT+CGG 0.618191 4.3:+51625254 None:intergenic
TTAAACCAGAGGGATAAAGC+CGG 0.622605 4.3:-51625537 MS.gene071113:CDS
ACTAGCGGCAAACCAACCCA+TGG 0.623120 4.3:+51624848 None:intergenic
AGTACTTGCTATGTCTAATG+CGG 0.642109 4.3:+51625316 None:intergenic
CCTCACAGTCAAGTATTCAA+GGG 0.642621 4.3:+51625677 None:intergenic
ATTCTCGCACACAGGCACAA+AGG 0.644231 4.3:-51625235 MS.gene071113:CDS
ATAGATGCAGCAATATGTAA+CGG 0.652232 4.3:+51625951 None:intergenic
GCTTGGAAGCTAAATCCGCA+GGG 0.653006 4.3:-51625879 MS.gene071113:CDS
AAAATGGAATATTAAACCAG+AGG 0.659194 4.3:-51625548 MS.gene071113:CDS
AGTCACTGATTCTCGCACAC+AGG 0.664845 4.3:-51625243 MS.gene071113:CDS
CATCTCCGATTACTGCGTAG+AGG 0.674210 4.3:+51626021 None:intergenic
CTAGCGGCAAACCAACCCAT+GGG 0.674900 4.3:+51624849 None:intergenic
AACCTGATAAATTCCACCAG+GGG 0.693447 4.3:+51625070 None:intergenic
TAACCTGATAAATTCCACCA+GGG 0.695982 4.3:+51625069 None:intergenic
ACCTGATAAATTCCACCAGG+GGG 0.706697 4.3:+51625071 None:intergenic
GTTAGTGCCAATGATGACAG+CGG 0.707440 4.3:-51625050 MS.gene071113:CDS
CATTAGAAGTAATATAACAG+AGG 0.709657 4.3:-51625162 MS.gene071113:CDS
TGAATGGGACTGAATCCCTG+CGG 0.749640 4.3:+51625864 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTGTTAATTAATTTCT+TGG - chr4.3:51626097-51626116 MS.gene071113:intron 10.0%
!!! TTTTAAATTCATAATTTCAA+TGG + chr4.3:51625597-51625616 None:intergenic 10.0%
!! GAAATCTGAAAATTAATTAA+GGG - chr4.3:51625026-51625045 MS.gene071113:CDS 15.0%
!! TTTGACTATTAATATCTTTA+AGG + chr4.3:51625070-51625089 None:intergenic 15.0%
!!! AACGAAACTTTTATTAATTA+AGG - chr4.3:51624994-51625013 MS.gene071113:CDS 15.0%
!!! AATTAATAAAAGTTTCGTTT+TGG + chr4.3:51624993-51625012 None:intergenic 15.0%
!!! ACGAAACTTTTATTAATTAA+GGG - chr4.3:51624995-51625014 MS.gene071113:CDS 15.0%
!! AAAGATAAAACAAAAGGTTT+CGG - chr4.3:51625204-51625223 MS.gene071113:CDS 20.0%
!! AGAAATCAAGAAAGATATAA+AGG + chr4.3:51626070-51626089 None:intergenic 20.0%
!! CGAAATCTGAAAATTAATTA+AGG - chr4.3:51625025-51625044 MS.gene071113:CDS 20.0%
!! TAATAGTCAAAAATGTGTAT+TGG - chr4.3:51625079-51625098 MS.gene071113:CDS 20.0%
!! TAATATATTGAATTACCAAG+AGG - chr4.3:51625322-51625341 MS.gene071113:CDS 20.0%
!! TTCATAATTTCAATGGTAAA+CGG + chr4.3:51625590-51625609 None:intergenic 20.0%
!! TTTACGAAAGATAAAACAAA+AGG - chr4.3:51625198-51625217 MS.gene071113:CDS 20.0%
!!! AAGAGTATATTATTAAGTGT+TGG - chr4.3:51626221-51626240 MS.gene071113:intron 20.0%
!!! AGAAAGACACTATATATTTT+TGG + chr4.3:51625685-51625704 None:intergenic 20.0%
!!! ATCTTTTTAAAATTAACGAG+AGG - chr4.3:51624933-51624952 MS.gene071113:intron 20.0%
!!! TAACAAAATAGTTCTTTTTG+TGG + chr4.3:51626309-51626328 None:intergenic 20.0%
!!! TCTTTTTAAAATTAACGAGA+GGG - chr4.3:51624934-51624953 MS.gene071113:intron 20.0%
!!! TTAATTTTCAGATTTCGTTT+TGG + chr4.3:51625022-51625041 None:intergenic 20.0%
! AAAATGGAATATTAAACCAG+AGG - chr4.3:51625957-51625976 MS.gene071113:CDS 25.0%
! AAATGGAATATTAAACCAGA+GGG - chr4.3:51625958-51625977 MS.gene071113:CDS 25.0%
! AATAATATACTCTTGATCTC+TGG + chr4.3:51626215-51626234 None:intergenic 25.0%
! CATTAGAAGTAATATAACAG+AGG - chr4.3:51626343-51626362 MS.gene071113:intron 25.0%
! CGCTAAAATTGATACAAAAA+AGG + chr4.3:51626588-51626607 None:intergenic 25.0%
! TAAAAAAATACACTGTGCAT+GGG - chr4.3:51625290-51625309 MS.gene071113:CDS 25.0%
! TAATTGTTATAGGATAGAGT+GGG - chr4.3:51625149-51625168 MS.gene071113:CDS 25.0%
! TCATAATTTCAATGGTAAAC+GGG + chr4.3:51625589-51625608 None:intergenic 25.0%
! TGTTAAGAATCAACAATATG+AGG - chr4.3:51625874-51625893 MS.gene071113:CDS 25.0%
! TTAAAAAAATACACTGTGCA+TGG - chr4.3:51625289-51625308 MS.gene071113:CDS 25.0%
! TTAAGGCTTAATTACACATT+TGG + chr4.3:51625053-51625072 None:intergenic 25.0%
! TTATTGAATAAGATTACCTG+CGG + chr4.3:51626031-51626050 None:intergenic 25.0%
!! ATTTAAAACCTTCATTTGCT+TGG - chr4.3:51625609-51625628 MS.gene071113:CDS 25.0%
!! TTGCGACATGTATTTTATAA+CGG - chr4.3:51625120-51625139 MS.gene071113:CDS 25.0%
!!! AATTTTAAAAAGATGACCGT+TGG + chr4.3:51624927-51624946 None:intergenic 25.0%
AAAGACCTTGTTAGAGTTAA+AGG - chr4.3:51625719-51625738 MS.gene071113:CDS 30.0%
ATAGATGCAGCAATATGTAA+CGG + chr4.3:51625557-51625576 None:intergenic 30.0%
ATATATGTTATGATACCAGC+CGG - chr4.3:51626608-51626627 MS.gene071113:intron 30.0%
GTAATTGTTATAGGATAGAG+TGG - chr4.3:51625148-51625167 MS.gene071113:CDS 30.0%
TAGGGTAGAAATAGTAGAAT+TGG + chr4.3:51626498-51626517 None:intergenic 30.0%
TATTGAATTACCAAGAGGTA+CGG - chr4.3:51625327-51625346 MS.gene071113:CDS 30.0%
TTACATATTGCTGCATCTAT+GGG - chr4.3:51625557-51625576 MS.gene071113:CDS 30.0%
! AATTAATTTCTTGGTAGCAC+AGG - chr4.3:51626106-51626125 MS.gene071113:intron 30.0%
! ACTAACAGAAGCACATTTAA+GGG - chr4.3:51624966-51624985 MS.gene071113:intron 30.0%
! ATTTGTGTTCAGGTTTGAAA+CGG + chr4.3:51625417-51625436 None:intergenic 30.0%
! CAACACCATTTTGCATATTT+CGG - chr4.3:51625991-51626010 MS.gene071113:CDS 30.0%
! CTTCATTTTGCAAGTCAAAA+TGG - chr4.3:51625761-51625780 MS.gene071113:CDS 30.0%
!! AATTCTACTATTTCTACCCT+AGG - chr4.3:51626497-51626516 MS.gene071113:intron 30.0%
!! GACATGTATTTTATAACGGA+TGG - chr4.3:51625124-51625143 MS.gene071113:CDS 30.0%
!!! TTTTGACTTGCAAAATGAAG+AGG + chr4.3:51625761-51625780 None:intergenic 30.0%
ACGGATGGAGTAATTGTTAT+AGG - chr4.3:51625139-51625158 MS.gene071113:CDS 35.0%
AGAAGAAAAGGATGATCCAA+CGG - chr4.3:51624908-51624927 MS.gene071113:intron 35.0%
ATGAAGATGATGAAGAAGAG+AGG - chr4.3:51624872-51624891 MS.gene071113:CDS 35.0%
CCAGCTAACAAGTATTTGAA+GGG + chr4.3:51625900-51625919 None:intergenic 35.0%
CTCAAACGAAATAATCGAAG+AGG - chr4.3:51625920-51625939 MS.gene071113:CDS 35.0%
CTCTTCCTTTAACTCTAACA+AGG + chr4.3:51625727-51625746 None:intergenic 35.0%
GCTCAGATATATTTGTGTTC+AGG + chr4.3:51625427-51625446 None:intergenic 35.0%
GGAATTTCTCTTTCTTTGTC+CGG - chr4.3:51625802-51625821 MS.gene071113:CDS 35.0%
GGTAGAAATAGTAGAATTGG+CGG + chr4.3:51626495-51626514 None:intergenic 35.0%
GGTTCTCAAAAGTTGGAAAA+TGG - chr4.3:51625941-51625960 MS.gene071113:CDS 35.0%
GTTACATATTGCTGCATCTA+TGG - chr4.3:51625556-51625575 MS.gene071113:CDS 35.0%
GTTAGAGTTAAAGGAAGAGA+GGG - chr4.3:51625728-51625747 MS.gene071113:CDS 35.0%
TAACCTGATAAATTCCACCA+GGG + chr4.3:51626439-51626458 None:intergenic 35.0%
TCTATATGCTCCAGAATGAA+TGG + chr4.3:51625512-51625531 None:intergenic 35.0%
TCTTTCGTAAAACAACTACC+CGG + chr4.3:51625189-51625208 None:intergenic 35.0%
TGTTAGAGTTAAAGGAAGAG+AGG - chr4.3:51625727-51625746 MS.gene071113:CDS 35.0%
TTTAGCAATGTGCGAAAGAT+AGG + chr4.3:51625388-51625407 None:intergenic 35.0%
! CTTCTATTGTTGGTAAGTAC+TGG - chr4.3:51626131-51626150 MS.gene071113:intron 35.0%
! GACTAACAGAAGCACATTTA+AGG - chr4.3:51624965-51624984 MS.gene071113:intron 35.0%
! TAGTGCCGAAATATGCAAAA+TGG + chr4.3:51625999-51626018 None:intergenic 35.0%
! TGTAACGGAGTTTCAACAAA+TGG + chr4.3:51625542-51625561 None:intergenic 35.0%
! TTTAGCTTCCAAGCAAATGA+AGG + chr4.3:51625620-51625639 None:intergenic 35.0%
! TTTTGCATAGCGAGATGAAT+GGG + chr4.3:51625659-51625678 None:intergenic 35.0%
!! AATATGCAAAATGGTGTTGC+CGG + chr4.3:51625990-51626009 None:intergenic 35.0%
!! AGTACTTGCTATGTCTAATG+CGG + chr4.3:51626192-51626211 None:intergenic 35.0%
!! GGTAAGTACTGGTATGAATT+TGG - chr4.3:51626142-51626161 MS.gene071113:intron 35.0%
!!! GTGGGTATTATTTTAACACC+CGG - chr4.3:51625167-51625186 MS.gene071113:CDS 35.0%
!!! TGGGTATTATTTTAACACCC+GGG - chr4.3:51625168-51625187 MS.gene071113:CDS 35.0%
AACCTGATAAATTCCACCAG+GGG + chr4.3:51626438-51626457 None:intergenic 40.0%
AAGATGATGAAGAAGAGAGG+AGG - chr4.3:51624875-51624894 MS.gene071113:CDS 40.0%
ACCTCACAGTCAAGTATTCA+AGG + chr4.3:51625832-51625851 None:intergenic 40.0%
ATTTCTACCCTAGGAAATGC+TGG - chr4.3:51626506-51626525 MS.gene071113:intron 40.0%
CCTCACAGTCAAGTATTCAA+GGG + chr4.3:51625831-51625850 None:intergenic 40.0%
CTAACCTGATAAATTCCACC+AGG + chr4.3:51626440-51626459 None:intergenic 40.0%
CTTTCGTAAAACAACTACCC+GGG + chr4.3:51625188-51625207 None:intergenic 40.0%
GAATCAGTGACTTCTAAGCT+CGG + chr4.3:51626254-51626273 None:intergenic 40.0%
GAATTTGGACGCAAAGAATG+AGG - chr4.3:51626157-51626176 MS.gene071113:intron 40.0%
GCCAGCTAACAAGTATTTGA+AGG + chr4.3:51625901-51625920 None:intergenic 40.0%
GGAGGAAGAAGAAGAAGAAA+AGG - chr4.3:51624896-51624915 MS.gene071113:CDS 40.0%
GTCAAGTATTCAAGGGAATC+CGG + chr4.3:51625824-51625843 None:intergenic 40.0%
TACTAGAGAGCCTAGTATTC+CGG + chr4.3:51626630-51626649 None:intergenic 40.0%
TCGAAGAGGTTCTCAAAAGT+TGG - chr4.3:51625934-51625953 MS.gene071113:CDS 40.0%
TGATTTCCCAGCATTTCCTA+GGG + chr4.3:51626516-51626535 None:intergenic 40.0%
TTAAACCAGAGGGATAAAGC+CGG - chr4.3:51625968-51625987 MS.gene071113:CDS 40.0%
TTTCTACCCTAGGAAATGCT+GGG - chr4.3:51626507-51626526 MS.gene071113:intron 40.0%
! CCCTTCAAATACTTGTTAGC+TGG - chr4.3:51625897-51625916 MS.gene071113:CDS 40.0%
! GTTTTGCATAGCGAGATGAA+TGG + chr4.3:51625660-51625679 None:intergenic 40.0%
! TTTTGCAAGTCAAAATGGCG+AGG - chr4.3:51625766-51625785 MS.gene071113:CDS 40.0%
!! TGGCGAGGTTGAATTTTTAG+CGG - chr4.3:51625781-51625800 MS.gene071113:CDS 40.0%
ACCTGATAAATTCCACCAGG+GGG + chr4.3:51626437-51626456 None:intergenic 45.0%
AGCACAGGCACTTCTATTGT+TGG - chr4.3:51626121-51626140 MS.gene071113:intron 45.0%
ATGATACCAGCCGGAATACT+AGG - chr4.3:51626617-51626636 MS.gene071113:intron 45.0%
ATGATGAAGAAGAGAGGAGG+AGG - chr4.3:51624878-51624897 MS.gene071113:CDS 45.0%
CAATTTCTCACCGTACCTCT+TGG + chr4.3:51625340-51625359 None:intergenic 45.0%
CCCTTGAATACTTGACTGTG+AGG - chr4.3:51625828-51625847 MS.gene071113:CDS 45.0%
CGGAGATGATCCATTCATTC+TGG - chr4.3:51625499-51625518 MS.gene071113:CDS 45.0%
CTGATTTCCCAGCATTTCCT+AGG + chr4.3:51626517-51626536 None:intergenic 45.0%
GATCTCCTCTACGCAGTAAT+CGG - chr4.3:51625479-51625498 MS.gene071113:intron 45.0%
GCAACTGCGATCTATCAATC+CGG - chr4.3:51626404-51626423 MS.gene071113:intron 45.0%
GGAATACTAGGCTCTCTAGT+AGG - chr4.3:51626629-51626648 MS.gene071113:intron 45.0%
GTTAGTGCCAATGATGACAG+CGG - chr4.3:51626455-51626474 MS.gene071113:intron 45.0%
TCTCTAGTAGGTTCTCCCAT+GGG - chr4.3:51626641-51626660 MS.gene071113:intron 45.0%
!! GCTGGGAAATCAGTTTTGTC+AGG - chr4.3:51626524-51626543 MS.gene071113:intron 45.0%
ATTCTCGCACACAGGCACAA+AGG - chr4.3:51626270-51626289 MS.gene071113:intron 50.0%
CATCTCCGATTACTGCGTAG+AGG + chr4.3:51625487-51625506 None:intergenic 50.0%
CTCTCTAGTAGGTTCTCCCA+TGG - chr4.3:51626640-51626659 MS.gene071113:intron 50.0%
GCTTGGAAGCTAAATCCGCA+GGG - chr4.3:51625626-51625645 MS.gene071113:CDS 50.0%
TAGTAGGTTCTCCCATGGGT+TGG - chr4.3:51626645-51626664 MS.gene071113:intron 50.0%
TCCCCCTGGTGGAATTTATC+AGG - chr4.3:51626433-51626452 MS.gene071113:intron 50.0%
TGAATGGGACTGAATCCCTG+CGG + chr4.3:51625644-51625663 None:intergenic 50.0%
TGCTTGGAAGCTAAATCCGC+AGG - chr4.3:51625625-51625644 MS.gene071113:CDS 50.0%
!! AGTCACTGATTCTCGCACAC+AGG - chr4.3:51626262-51626281 MS.gene071113:intron 50.0%
AGAGAGCCTAGTATTCCGGC+TGG + chr4.3:51626626-51626645 None:intergenic 55.0%
CAGAGGAGCAACGCAACACT+TGG - chr4.3:51626360-51626379 MS.gene071113:intron 55.0%
CTAGCGGCAAACCAACCCAT+GGG + chr4.3:51626659-51626678 None:intergenic 55.0%
CTGAAAGCTGCAGCTCAGGA+AGG - chr4.3:51625449-51625468 MS.gene071113:intron 55.0%
GTTCACGCCGCTGTCATCAT+TGG + chr4.3:51626465-51626484 None:intergenic 55.0%
!! TGTTGCCGGCTTTATCCCTC+TGG + chr4.3:51625976-51625995 None:intergenic 55.0%
CAATCCGGATTGAGTCCCCC+TGG - chr4.3:51626419-51626438 MS.gene071113:intron 60.0%
GCACACAGGCACAAAGGCTC+AGG - chr4.3:51626276-51626295 MS.gene071113:intron 60.0%
GCAGCTGAAAGCTGCAGCTC+AGG - chr4.3:51625445-51625464 MS.gene071113:intron 60.0%
GGCACTAAGTTGTGAGCCGC+AGG - chr4.3:51626012-51626031 MS.gene071113:CDS 60.0%
TCCACCAGGGGGACTCAATC+CGG + chr4.3:51626426-51626445 None:intergenic 60.0%
TCCGGATTGAGTCCCCCTGG+TGG - chr4.3:51626422-51626441 MS.gene071113:intron 65.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 51624849 51626678 51624849 ID=MS.gene071113
chr4.3 mRNA 51624849 51626678 51624849 ID=MS.gene071113.t1;Parent=MS.gene071113
chr4.3 exon 51626676 51626678 51626676 ID=MS.gene071113.t1.exon1;Parent=MS.gene071113.t1
chr4.3 CDS 51626676 51626678 51626676 ID=cds.MS.gene071113.t1;Parent=MS.gene071113.t1
chr4.3 exon 51625494 51626078 51625494 ID=MS.gene071113.t1.exon2;Parent=MS.gene071113.t1
chr4.3 CDS 51625494 51626078 51625494 ID=cds.MS.gene071113.t1;Parent=MS.gene071113.t1
chr4.3 exon 51624987 51625399 51624987 ID=MS.gene071113.t1.exon3;Parent=MS.gene071113.t1
chr4.3 CDS 51624987 51625399 51624987 ID=cds.MS.gene071113.t1;Parent=MS.gene071113.t1
chr4.3 exon 51624849 51624921 51624849 ID=MS.gene071113.t1.exon4;Parent=MS.gene071113.t1
chr4.3 CDS 51624849 51624921 51624849 ID=cds.MS.gene071113.t1;Parent=MS.gene071113.t1
Gene Sequence

>MS.gene071113

ATGGTATGCTTTGATGAAGGTCTATGAAGATGATGAAGAAGAGAGGAGGAGGAAGAAGAAGAAGAAAAGGATGATCCAACGGTCATCTTTTTAAAATTAACGAGAGGGACTAGATTGACTAACAGAAGCACATTTAAGGGACCAAAACGAAACTTTTATTAATTAAGGGACCAAAACGAAATCTGAAAATTAATTAAGGGACCAAATGTGTAATTAAGCCTTAAAGATATTAATAGTCAAAAATGTGTATTGGCATGTGTGCAGTAATTGATTGCGACATGTATTTTATAACGGATGGAGTAATTGTTATAGGATAGAGTGGGTATTATTTTAACACCCGGGTAGTTGTTTTACGAAAGATAAAACAAAAGGTTTCGGAATCTGAGACATTGCATACATGTGAAACTCACACAAGTCAATGAATGTAATAAACAAAACATTTAAAAAAATACACTGTGCATGGGTTAATGAAATAATATATTGAATTACCAAGAGGTACGGTGAGAAATTGTTTTAATAGCAATAGCAGCTGAGAACCTATCTTTCGCACATTGCTAAATAATTACCGTTTCAAACCTGAACACAAATATATCTGAGCAGCTGAAAGCTGCAGCTCAGGAAGGTGATATAGATCTCCTCTACGCAGTAATCGGAGATGATCCATTCATTCTGGAGCATATAGATTTGATACCATTTGTTGAAACTCCGTTACATATTGCTGCATCTATGGGTCATATCCCGTTTACCATTGAAATTATGAATTTAAAACCTTCATTTGCTTGGAAGCTAAATCCGCAGGGATTCAGTCCCATTCATCTCGCTATGCAAAACGACCAAAAATATATAGTGTCTTTCTTTGTGCACAACAATAAAGACCTTGTTAGAGTTAAAGGAAGAGAGGGCTTAACTCCTCTTCATTTTGCAAGTCAAAATGGCGAGGTTGAATTTTTAGCGGAATTTCTCTTTCTTTGTCCGGATTCCCTTGAATACTTGACTGTGAGGTGCGAGACTGCACTGCATATTGCTGTTAAGAATCAACAATATGAGGCCCTTCAAATACTTGTTAGCTGGCTCAAACGAAATAATCGAAGAGGTTCTCAAAAGTTGGAAAATGGAATATTAAACCAGAGGGATAAAGCCGGCAACACCATTTTGCATATTTCGGCACTAAGTTGTGAGCCGCAGGTAATCTTATTCAATAACTAAAATCACATGTCTCCTTTATATCTTTCTTGATTTCTTGTATTGTTATTTGTTAATTAATTTCTTGGTAGCACAGGCACTTCTATTGTTGGTAAGTACTGGTATGAATTTGGACGCAAAGAATGAGGAGAACAAAACCGCATTAGACATAGCAAGTACTCCAGAGATCAAGAGTATATTATTAAGTGTTGGAGCAAAACCGAGCTTAGAAGTCACTGATTCTCGCACACAGGCACAAAGGCTCAGGTTAAAGACCACAAAAAGAACTATTTTGTTAACTTTTGTAAATCGCATTAGAAGTAATATAACAGAGGAGCAACGCAACACTTGGTTGATAGTTTCAACTCTTGTTGCAACTGCGATCTATCAATCCGGATTGAGTCCCCCTGGTGGAATTTATCAGGTTAGTGCCAATGATGACAGCGGCGTGAACAACACTTCCGCCAATTCTACTATTTCTACCCTAGGAAATGCTGGGAAATCAGTTTTGTCAGGATATGAATTCTTTTTGTTTTTATTTATAAATATGTATTCCTTTTTTGTATCAATTTTAGCGATATATGTTATGATACCAGCCGGAATACTAGGCTCTCTAGTAGGTTCTCCCATGGGTTGGTTTGCCGCTAG
Protein sequence

>MS.gene071113.t1

MLKAAAQEGDIDLLYAVIGDDPFILEHIDLIPFVETPLHIAASMGHIPFTIEIMNLKPSFAWKLNPQGFSPIHLAMQNDQKYIVSFFVHNNKDLVRVKGREGLTPLHFASQNGEVEFLAEFLFLCPDSLEYLTVRCETALHIAVKNQQYEALQILVSWLKRNNRRGSQKLENGILNQRDKAGNTILHISALSCEPQALLLLVSTGMNLDAKNEENKTALDIASTPEIKSILLSVGAKPSLEVTDSRTQAQRLRLKTTKRTILLTFVNRIRSNITEEQRNTWLIVSTLVATAIYQSGLSPPGGIYQVSANDDSGVNNTSANSTISTLGNAGKSVFDICYDTSRNTRLSSRFSHGLVCR