AlfalfaGEDB | MS.gene071113

M. sativa cultivar XinJiangDaYe / MS.gene071113

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF12796.10 PF13962.9

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene071113.t1	AES77856.1	59.9	332	127	1	2	333	9	334	2.00E-92	349.4

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene071113.t1	Q8GYH5	38.9	301	175	3	2	295	5	303	2.9e-15	84.3

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene071113.t1	G7KSX2	59.9	332	127	1	2	333	9	334	1.5e-92	349.4

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
MS.gene005995	MS.gene071113	PPI
MS.gene010910	MS.gene071113	PPI
MS.gene010912	MS.gene071113	PPI
MS.gene027071	MS.gene071113	PPI
MS.gene027074	MS.gene071113	PPI
MS.gene035805	MS.gene071113	PPI
MS.gene037950	MS.gene071113	PPI
MS.gene039682	MS.gene071113	PPI
MS.gene056455	MS.gene071113	PPI
MS.gene063222	MS.gene071113	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene071113.t1	MTR_8g072510	76.577	333	77	1	2	334	10	341	0.0	510
MS.gene071113.t1	MTR_8g039620	70.605	347	90	3	2	337	14	359	2.98e-170	481
MS.gene071113.t1	MTR_7g021090	71.471	333	89	1	2	334	9	335	1.72e-168	476
MS.gene071113.t1	MTR_8g062410	73.353	334	81	2	2	334	10	336	2.55e-164	467
MS.gene071113.t1	MTR_1g059930	68.468	333	105	0	2	334	12	344	4.55e-161	458
MS.gene071113.t1	MTR_3g016210	65.341	352	106	3	2	338	10	360	3.61e-156	446
MS.gene071113.t1	MTR_8g068990	64.058	345	102	5	2	341	12	339	7.23e-148	425
MS.gene071113.t1	MTR_7g451420	67.763	304	98	0	2	305	9	312	9.63e-144	409
MS.gene071113.t1	MTR_8g073260	61.677	334	121	2	2	333	11	339	1.09e-143	414
MS.gene071113.t1	MTR_8g069880	62.575	334	122	3	1	333	6	337	4.91e-136	395

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene071113.t1	AT4G10720	41.379	319	179	2	2	312	5	323	2.17e-78	246
MS.gene071113.t1	AT4G10720	41.379	319	179	2	2	312	5	323	5.21e-78	246
MS.gene071113.t1	AT5G15500	39.222	334	186	6	2	323	6	334	5.65e-73	234
MS.gene071113.t1	AT1G14500	37.700	313	186	2	2	305	5	317	1.79e-72	232
MS.gene071113.t1	AT1G14480	37.771	323	190	3	2	313	5	327	2.06e-70	226
MS.gene071113.t1	AT5G54620	39.597	298	173	2	9	299	12	309	1.13e-67	219
MS.gene071113.t1	AT5G54610	36.027	297	181	3	7	296	10	304	5.67e-60	199
MS.gene071113.t1	AT1G14480	36.624	314	163	5	2	313	5	284	7.36e-55	185
MS.gene071113.t1	AT5G15500	33.906	233	137	6	103	323	1	228	1.85e-32	124
MS.gene071113.t1	AT5G51160	31.022	274	161	5	56	308	35	301	3.82e-31	122

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Find 77 sgRNAs with CRISPR-Local

Find 134 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATGGAATATTAAACCAG+AGG	0.659194	4.3:-51625548	MS.gene071113:CDS
AAAGACCTTGTTAGAGTTAA+AGG	0.117136	4.3:-51625786	MS.gene071113:CDS
AAATGGAATATTAAACCAGA+GGG	0.585975	4.3:-51625547	MS.gene071113:CDS
AACCTGATAAATTCCACCAG+GGG	0.693447	4.3:+51625070	None:intergenic
AAGAGTATATTATTAAGTGT+TGG	0.527914	4.3:-51625284	MS.gene071113:CDS
AATAATATACTCTTGATCTC+TGG	0.484204	4.3:+51625293	None:intergenic
AATATGCAAAATGGTGTTGC+CGG	0.418241	4.3:+51625518	None:intergenic
AATTCTACTATTTCTACCCT+AGG	0.517193	4.3:-51625008	MS.gene071113:CDS
ACCTCACAGTCAAGTATTCA+AGG	0.520683	4.3:+51625676	None:intergenic
ACCTGATAAATTCCACCAGG+GGG	0.706697	4.3:+51625071	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAGACCTTGTTAGAGTTAA+AGG	-	chr4.3:51625719-51625738	MS.gene071113:CDS	30.0%
ATAGATGCAGCAATATGTAA+CGG	+	chr4.3:51625557-51625576	None:intergenic	30.0%
ATATATGTTATGATACCAGC+CGG	-	chr4.3:51626608-51626627	MS.gene071113:intron	30.0%
GTAATTGTTATAGGATAGAG+TGG	-	chr4.3:51625148-51625167	MS.gene071113:CDS	30.0%
TAGGGTAGAAATAGTAGAAT+TGG	+	chr4.3:51626498-51626517	None:intergenic	30.0%
TATTGAATTACCAAGAGGTA+CGG	-	chr4.3:51625327-51625346	MS.gene071113:CDS	30.0%
TTACATATTGCTGCATCTAT+GGG	-	chr4.3:51625557-51625576	MS.gene071113:CDS	30.0%
ACGGATGGAGTAATTGTTAT+AGG	-	chr4.3:51625139-51625158	MS.gene071113:CDS	35.0%
AGAAGAAAAGGATGATCCAA+CGG	-	chr4.3:51624908-51624927	MS.gene071113:intron	35.0%
ATGAAGATGATGAAGAAGAG+AGG	-	chr4.3:51624872-51624891	MS.gene071113:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	51624849	51626678	51624849	ID=MS.gene071113
chr4.3	mRNA	51624849	51626678	51624849	ID=MS.gene071113.t1;Parent=MS.gene071113
chr4.3	exon	51626676	51626678	51626676	ID=MS.gene071113.t1.exon1;Parent=MS.gene071113.t1
chr4.3	CDS	51626676	51626678	51626676	ID=cds.MS.gene071113.t1;Parent=MS.gene071113.t1
chr4.3	exon	51625494	51626078	51625494	ID=MS.gene071113.t1.exon2;Parent=MS.gene071113.t1
chr4.3	CDS	51625494	51626078	51625494	ID=cds.MS.gene071113.t1;Parent=MS.gene071113.t1
chr4.3	exon	51624987	51625399	51624987	ID=MS.gene071113.t1.exon3;Parent=MS.gene071113.t1
chr4.3	CDS	51624987	51625399	51624987	ID=cds.MS.gene071113.t1;Parent=MS.gene071113.t1
chr4.3	exon	51624849	51624921	51624849	ID=MS.gene071113.t1.exon4;Parent=MS.gene071113.t1
chr4.3	CDS	51624849	51624921	51624849	ID=cds.MS.gene071113.t1;Parent=MS.gene071113.t1

Gene Sequence

>MS.gene071113

ATGGTATGCTTTGATGAAGGTCTATGAAGATGATGAAGAAGAGAGGAGGAGGAAGAAGAAGAAGAAAAGGATGATCCAACGGTCATCTTTTTAAAATTAACGAGAGGGACTAGATTGACTAACAGAAGCACATTTAAGGGACCAAAACGAAACTTTTATTAATTAAGGGACCAAAACGAAATCTGAAAATTAATTAAGGGACCAAATGTGTAATTAAGCCTTAAAGATATTAATAGTCAAAAATGTGTATTGGCATGTGTGCAGTAATTGATTGCGACATGTATTTTATAACGGATGGAGTAATTGTTATAGGATAGAGTGGGTATTATTTTAACACCCGGGTAGTTGTTTTACGAAAGATAAAACAAAAGGTTTCGGAATCTGAGACATTGCATACATGTGAAACTCACACAAGTCAATGAATGTAATAAACAAAACATTTAAAAAAATACACTGTGCATGGGTTAATGAAATAATATATTGAATTACCAAGAGGTACGGTGAGAAATTGTTTTAATAGCAATAGCAGCTGAGAACCTATCTTTCGCACATTGCTAAATAATTACCGTTTCAAACCTGAACACAAATATATCTGAGCAGCTGAAAGCTGCAGCTCAGGAAGGTGATATAGATCTCCTCTACGCAGTAATCGGAGATGATCCATTCATTCTGGAGCATATAGATTTGATACCATTTGTTGAAACTCCGTTACATATTGCTGCATCTATGGGTCATATCCCGTTTACCATTGAAATTATGAATTTAAAACCTTCATTTGCTTGGAAGCTAAATCCGCAGGGATTCAGTCCCATTCATCTCGCTATGCAAAACGACCAAAAATATATAGTGTCTTTCTTTGTGCACAACAATAAAGACCTTGTTAGAGTTAAAGGAAGAGAGGGCTTAACTCCTCTTCATTTTGCAAGTCAAAATGGCGAGGTTGAATTTTTAGCGGAATTTCTCTTTCTTTGTCCGGATTCCCTTGAATACTTGACTGTGAGGTGCGAGACTGCACTGCATATTGCTGTTAAGAATCAACAATATGAGGCCCTTCAAATACTTGTTAGCTGGCTCAAACGAAATAATCGAAGAGGTTCTCAAAAGTTGGAAAATGGAATATTAAACCAGAGGGATAAAGCCGGCAACACCATTTTGCATATTTCGGCACTAAGTTGTGAGCCGCAGGTAATCTTATTCAATAACTAAAATCACATGTCTCCTTTATATCTTTCTTGATTTCTTGTATTGTTATTTGTTAATTAATTTCTTGGTAGCACAGGCACTTCTATTGTTGGTAAGTACTGGTATGAATTTGGACGCAAAGAATGAGGAGAACAAAACCGCATTAGACATAGCAAGTACTCCAGAGATCAAGAGTATATTATTAAGTGTTGGAGCAAAACCGAGCTTAGAAGTCACTGATTCTCGCACACAGGCACAAAGGCTCAGGTTAAAGACCACAAAAAGAACTATTTTGTTAACTTTTGTAAATCGCATTAGAAGTAATATAACAGAGGAGCAACGCAACACTTGGTTGATAGTTTCAACTCTTGTTGCAACTGCGATCTATCAATCCGGATTGAGTCCCCCTGGTGGAATTTATCAGGTTAGTGCCAATGATGACAGCGGCGTGAACAACACTTCCGCCAATTCTACTATTTCTACCCTAGGAAATGCTGGGAAATCAGTTTTGTCAGGATATGAATTCTTTTTGTTTTTATTTATAAATATGTATTCCTTTTTTGTATCAATTTTAGCGATATATGTTATGATACCAGCCGGAATACTAGGCTCTCTAGTAGGTTCTCCCATGGGTTGGTTTGCCGCTAG

Protein sequence

>MS.gene071113.t1

MLKAAAQEGDIDLLYAVIGDDPFILEHIDLIPFVETPLHIAASMGHIPFTIEIMNLKPSFAWKLNPQGFSPIHLAMQNDQKYIVSFFVHNNKDLVRVKGREGLTPLHFASQNGEVEFLAEFLFLCPDSLEYLTVRCETALHIAVKNQQYEALQILVSWLKRNNRRGSQKLENGILNQRDKAGNTILHISALSCEPQALLLLVSTGMNLDAKNEENKTALDIASTPEIKSILLSVGAKPSLEVTDSRTQAQRLRLKTTKRTILLTFVNRIRSNITEEQRNTWLIVSTLVATAIYQSGLSPPGGIYQVSANDDSGVNNTSANSTISTLGNAGKSVFDICYDTSRNTRLSSRFSHGLVCR