Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32274.t1 | XP_013445472.1 | 95.6 | 227 | 10 | 0 | 1 | 227 | 1 | 227 | 2.30E-121 | 444.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32274.t1 | Q9Y5Z4 | 34.4 | 186 | 106 | 5 | 38 | 217 | 20 | 195 | 1.3e-21 | 104.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32274.t1 | I3S399 | 95.6 | 227 | 10 | 0 | 1 | 227 | 1 | 227 | 1.6e-121 | 444.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049284 | MS.gene32274 | 0.817658 | 2.94E-52 | -1.69E-46 |
| MS.gene049351 | MS.gene32274 | 0.836826 | 7.45E-57 | -1.69E-46 |
| MS.gene049356 | MS.gene32274 | 0.852875 | 3.46E-61 | -1.69E-46 |
| MS.gene049369 | MS.gene32274 | 0.858327 | 8.89E-63 | -1.69E-46 |
| MS.gene049427 | MS.gene32274 | 0.86282 | 3.87E-64 | -1.69E-46 |
| MS.gene049491 | MS.gene32274 | 0.837653 | 4.58E-57 | -1.69E-46 |
| MS.gene049614 | MS.gene32274 | 0.824676 | 7.09E-54 | -1.69E-46 |
| MS.gene049661 | MS.gene32274 | 0.859 | 5.60E-63 | -1.69E-46 |
| MS.gene049662 | MS.gene32274 | 0.858282 | 9.17E-63 | -1.69E-46 |
| MS.gene049758 | MS.gene32274 | 0.828056 | 1.11E-54 | -1.69E-46 |
| MS.gene049805 | MS.gene32274 | 0.831508 | 1.60E-55 | -1.69E-46 |
| MS.gene050076 | MS.gene32274 | 0.836777 | 7.67E-57 | -1.69E-46 |
| MS.gene050128 | MS.gene32274 | 0.81217 | 4.85E-51 | -1.69E-46 |
| MS.gene050301 | MS.gene32274 | 0.850594 | 1.54E-60 | -1.69E-46 |
| MS.gene050397 | MS.gene32274 | 0.850133 | 2.07E-60 | -1.69E-46 |
| MS.gene050432 | MS.gene32274 | 0.856128 | 3.97E-62 | -1.69E-46 |
| MS.gene050529 | MS.gene32274 | 0.837898 | 3.96E-57 | -1.69E-46 |
| MS.gene05053 | MS.gene32274 | 0.803605 | 3.22E-49 | -1.69E-46 |
| MS.gene050670 | MS.gene32274 | 0.829508 | 4.95E-55 | -1.69E-46 |
| MS.gene050767 | MS.gene32274 | 0.829579 | 4.76E-55 | -1.69E-46 |
| MS.gene050770 | MS.gene32274 | 0.835163 | 1.97E-56 | -1.69E-46 |
| MS.gene050772 | MS.gene32274 | 0.805073 | 1.59E-49 | -1.69E-46 |
| MS.gene050812 | MS.gene32274 | 0.816105 | 6.56E-52 | -1.69E-46 |
| MS.gene050971 | MS.gene32274 | 0.829357 | 5.39E-55 | -1.69E-46 |
| MS.gene05101 | MS.gene32274 | 0.838838 | 2.27E-57 | -1.69E-46 |
| MS.gene05105 | MS.gene32274 | 0.851822 | 6.91E-61 | -1.69E-46 |
| MS.gene051228 | MS.gene32274 | 0.812662 | 3.79E-51 | -1.69E-46 |
| MS.gene051255 | MS.gene32274 | 0.806508 | 7.95E-50 | -1.69E-46 |
| MS.gene051360 | MS.gene32274 | 0.806172 | 9.36E-50 | -1.69E-46 |
| MS.gene051387 | MS.gene32274 | 0.808158 | 3.55E-50 | -1.69E-46 |
| MS.gene051471 | MS.gene32274 | 0.849553 | 3.00E-60 | -1.69E-46 |
| MS.gene051605 | MS.gene32274 | 0.814217 | 1.72E-51 | -1.69E-46 |
| MS.gene051692 | MS.gene32274 | 0.816707 | 4.81E-52 | -1.69E-46 |
| MS.gene052078 | MS.gene32274 | 0.841999 | 3.38E-58 | -1.69E-46 |
| MS.gene052178 | MS.gene32274 | 0.828606 | 8.19E-55 | -1.69E-46 |
| MS.gene052188 | MS.gene32274 | 0.881826 | 1.74E-70 | -1.69E-46 |
| MS.gene052253 | MS.gene32274 | 0.85118 | 1.05E-60 | -1.69E-46 |
| MS.gene052290 | MS.gene32274 | 0.835431 | 1.68E-56 | -1.69E-46 |
| MS.gene052310 | MS.gene32274 | 0.901562 | 2.36E-78 | -1.69E-46 |
| MS.gene052322 | MS.gene32274 | 0.813925 | 2.00E-51 | -1.69E-46 |
| MS.gene052326 | MS.gene32274 | 0.81649 | 5.38E-52 | -1.69E-46 |
| MS.gene052340 | MS.gene32274 | 0.882643 | 8.77E-71 | -1.69E-46 |
| MS.gene052379 | MS.gene32274 | 0.846049 | 2.77E-59 | -1.69E-46 |
| MS.gene052421 | MS.gene32274 | 0.858232 | 9.49E-63 | -1.69E-46 |
| MS.gene052481 | MS.gene32274 | 0.807888 | 4.06E-50 | -1.69E-46 |
| MS.gene052484 | MS.gene32274 | 0.81672 | 4.78E-52 | -1.69E-46 |
| MS.gene052485 | MS.gene32274 | 0.834399 | 3.07E-56 | -1.69E-46 |
| MS.gene052615 | MS.gene32274 | 0.813162 | 2.94E-51 | -1.69E-46 |
| MS.gene052870 | MS.gene32274 | 0.824582 | 7.46E-54 | -1.69E-46 |
| MS.gene052872 | MS.gene32274 | 0.813384 | 2.63E-51 | -1.69E-46 |
| MS.gene053103 | MS.gene32274 | 0.8348 | 2.43E-56 | -1.69E-46 |
| MS.gene053104 | MS.gene32274 | 0.820601 | 6.29E-53 | -1.69E-46 |
| MS.gene05316 | MS.gene32274 | 0.830129 | 3.49E-55 | -1.69E-46 |
| MS.gene053209 | MS.gene32274 | 0.855919 | 4.57E-62 | -1.69E-46 |
| MS.gene053322 | MS.gene32274 | 0.828831 | 7.22E-55 | -1.69E-46 |
| MS.gene053384 | MS.gene32274 | 0.8316 | 1.52E-55 | -1.69E-46 |
| MS.gene053449 | MS.gene32274 | 0.827412 | 1.59E-54 | -1.69E-46 |
| MS.gene053557 | MS.gene32274 | 0.845197 | 4.72E-59 | -1.69E-46 |
| MS.gene053758 | MS.gene32274 | 0.878242 | 3.29E-69 | -1.69E-46 |
| MS.gene053813 | MS.gene32274 | 0.836163 | 1.10E-56 | -1.69E-46 |
| MS.gene053836 | MS.gene32274 | 0.81476 | 1.31E-51 | -1.69E-46 |
| MS.gene053912 | MS.gene32274 | 0.83376 | 4.43E-56 | -1.69E-46 |
| MS.gene054123 | MS.gene32274 | 0.857837 | 1.24E-62 | -1.69E-46 |
| MS.gene054349 | MS.gene32274 | 0.815072 | 1.11E-51 | -1.69E-46 |
| MS.gene054376 | MS.gene32274 | 0.822416 | 2.40E-53 | -1.69E-46 |
| MS.gene054802 | MS.gene32274 | 0.82156 | 3.78E-53 | -1.69E-46 |
| MS.gene054803 | MS.gene32274 | 0.824612 | 7.35E-54 | -1.69E-46 |
| MS.gene054804 | MS.gene32274 | 0.824906 | 6.26E-54 | -1.69E-46 |
| MS.gene054955 | MS.gene32274 | 0.823263 | 1.52E-53 | -1.69E-46 |
| MS.gene05505 | MS.gene32274 | 0.861761 | 8.17E-64 | -1.69E-46 |
| MS.gene055116 | MS.gene32274 | 0.848284 | 6.76E-60 | -1.69E-46 |
| MS.gene055277 | MS.gene32274 | 0.883151 | 5.72E-71 | -1.69E-46 |
| MS.gene055390 | MS.gene32274 | 0.825091 | 5.66E-54 | -1.69E-46 |
| MS.gene055397 | MS.gene32274 | 0.842858 | 2.00E-58 | -1.69E-46 |
| MS.gene055412 | MS.gene32274 | 0.862536 | 4.73E-64 | -1.69E-46 |
| MS.gene055422 | MS.gene32274 | 0.838842 | 2.26E-57 | -1.69E-46 |
| MS.gene055428 | MS.gene32274 | 0.818299 | 2.11E-52 | -1.69E-46 |
| MS.gene055499 | MS.gene32274 | 0.803057 | 4.18E-49 | -1.69E-46 |
| MS.gene055722 | MS.gene32274 | 0.806504 | 7.97E-50 | -1.69E-46 |
| MS.gene055896 | MS.gene32274 | 0.81119 | 7.92E-51 | -1.69E-46 |
| MS.gene05592 | MS.gene32274 | 0.829578 | 4.76E-55 | -1.69E-46 |
| MS.gene056191 | MS.gene32274 | 0.826767 | 2.26E-54 | -1.69E-46 |
| MS.gene056314 | MS.gene32274 | 0.822526 | 2.26E-53 | -1.69E-46 |
| MS.gene056407 | MS.gene32274 | 0.816624 | 5.03E-52 | -1.69E-46 |
| MS.gene056410 | MS.gene32274 | 0.822462 | 2.34E-53 | -1.69E-46 |
| MS.gene056487 | MS.gene32274 | 0.811366 | 7.26E-51 | -1.69E-46 |
| MS.gene056511 | MS.gene32274 | 0.859526 | 3.89E-63 | -1.69E-46 |
| MS.gene056609 | MS.gene32274 | 0.893762 | 4.65E-75 | -1.69E-46 |
| MS.gene056612 | MS.gene32274 | 0.836131 | 1.12E-56 | -1.69E-46 |
| MS.gene056659 | MS.gene32274 | 0.852499 | 4.43E-61 | -1.69E-46 |
| MS.gene056719 | MS.gene32274 | 0.824769 | 6.75E-54 | -1.69E-46 |
| MS.gene056796 | MS.gene32274 | 0.800189 | 1.62E-48 | -1.69E-46 |
| MS.gene056897 | MS.gene32274 | 0.82269 | 2.07E-53 | -1.69E-46 |
| MS.gene057011 | MS.gene32274 | 0.848112 | 7.54E-60 | -1.69E-46 |
| MS.gene05702 | MS.gene32274 | 0.840229 | 9.86E-58 | -1.69E-46 |
| MS.gene057076 | MS.gene32274 | 0.816491 | 5.38E-52 | -1.69E-46 |
| MS.gene057123 | MS.gene32274 | 0.83521 | 1.92E-56 | -1.69E-46 |
| MS.gene057124 | MS.gene32274 | 0.842535 | 2.44E-58 | -1.69E-46 |
| MS.gene057129 | MS.gene32274 | 0.826613 | 2.46E-54 | -1.69E-46 |
| MS.gene057146 | MS.gene32274 | 0.839026 | 2.03E-57 | -1.69E-46 |
| MS.gene05733 | MS.gene32274 | 0.836235 | 1.05E-56 | -1.69E-46 |
| MS.gene05735 | MS.gene32274 | 0.849728 | 2.68E-60 | -1.69E-46 |
| MS.gene057416 | MS.gene32274 | 0.806389 | 8.42E-50 | -1.69E-46 |
| MS.gene057448 | MS.gene32274 | 0.827488 | 1.52E-54 | -1.69E-46 |
| MS.gene057449 | MS.gene32274 | 0.831981 | 1.23E-55 | -1.69E-46 |
| MS.gene057450 | MS.gene32274 | 0.839214 | 1.81E-57 | -1.69E-46 |
| MS.gene057749 | MS.gene32274 | 0.825384 | 4.83E-54 | -1.69E-46 |
| MS.gene057796 | MS.gene32274 | 0.809544 | 1.80E-50 | -1.69E-46 |
| MS.gene057823 | MS.gene32274 | 0.828809 | 7.31E-55 | -1.69E-46 |
| MS.gene057927 | MS.gene32274 | 0.834896 | 2.30E-56 | -1.69E-46 |
| MS.gene058002 | MS.gene32274 | 0.818074 | 2.37E-52 | -1.69E-46 |
| MS.gene058007 | MS.gene32274 | 0.819885 | 9.18E-53 | -1.69E-46 |
| MS.gene058082 | MS.gene32274 | 0.823776 | 1.15E-53 | -1.69E-46 |
| MS.gene05811 | MS.gene32274 | 0.840103 | 1.06E-57 | -1.69E-46 |
| MS.gene058130 | MS.gene32274 | 0.810641 | 1.04E-50 | -1.69E-46 |
| MS.gene058233 | MS.gene32274 | 0.819592 | 1.07E-52 | -1.69E-46 |
| MS.gene058293 | MS.gene32274 | 0.823261 | 1.52E-53 | -1.69E-46 |
| MS.gene058365 | MS.gene32274 | 0.851184 | 1.05E-60 | -1.69E-46 |
| MS.gene058660 | MS.gene32274 | 0.872942 | 2.16E-67 | -1.69E-46 |
| MS.gene058928 | MS.gene32274 | 0.898306 | 6.04E-77 | -1.69E-46 |
| MS.gene05907 | MS.gene32274 | 0.841572 | 4.38E-58 | -1.69E-46 |
| MS.gene059285 | MS.gene32274 | 0.805306 | 1.42E-49 | -1.69E-46 |
| MS.gene059294 | MS.gene32274 | 0.808707 | 2.71E-50 | -1.69E-46 |
| MS.gene059326 | MS.gene32274 | 0.815796 | 7.69E-52 | -1.69E-46 |
| MS.gene05934 | MS.gene32274 | 0.806181 | 9.32E-50 | -1.69E-46 |
| MS.gene059366 | MS.gene32274 | 0.848033 | 7.93E-60 | -1.69E-46 |
| MS.gene059420 | MS.gene32274 | 0.811124 | 8.19E-51 | -1.69E-46 |
| MS.gene059518 | MS.gene32274 | 0.802022 | 6.83E-49 | -1.69E-46 |
| MS.gene059536 | MS.gene32274 | 0.838296 | 3.13E-57 | -1.69E-46 |
| MS.gene059597 | MS.gene32274 | 0.86953 | 2.89E-66 | -1.69E-46 |
| MS.gene05959 | MS.gene32274 | 0.834087 | 3.67E-56 | -1.69E-46 |
| MS.gene059674 | MS.gene32274 | 0.821221 | 4.53E-53 | -1.69E-46 |
| MS.gene059676 | MS.gene32274 | 0.879995 | 7.90E-70 | -1.69E-46 |
| MS.gene059688 | MS.gene32274 | 0.889291 | 2.76E-73 | -1.69E-46 |
| MS.gene059692 | MS.gene32274 | 0.891247 | 4.73E-74 | -1.69E-46 |
| MS.gene059695 | MS.gene32274 | 0.886944 | 2.20E-72 | -1.69E-46 |
| MS.gene059708 | MS.gene32274 | 0.834931 | 2.25E-56 | -1.69E-46 |
| MS.gene059711 | MS.gene32274 | 0.854178 | 1.46E-61 | -1.69E-46 |
| MS.gene059807 | MS.gene32274 | 0.854411 | 1.25E-61 | -1.69E-46 |
| MS.gene05984 | MS.gene32274 | 0.8379 | 3.96E-57 | -1.69E-46 |
| MS.gene05986 | MS.gene32274 | 0.818732 | 1.68E-52 | -1.69E-46 |
| MS.gene059894 | MS.gene32274 | 0.872989 | 2.08E-67 | -1.69E-46 |
| MS.gene059960 | MS.gene32274 | 0.84417 | 8.92E-59 | -1.69E-46 |
| MS.gene059974 | MS.gene32274 | 0.835926 | 1.26E-56 | -1.69E-46 |
| MS.gene06037 | MS.gene32274 | 0.826765 | 2.27E-54 | -1.69E-46 |
| MS.gene060403 | MS.gene32274 | 0.80061 | 1.33E-48 | -1.69E-46 |
| MS.gene060407 | MS.gene32274 | 0.823948 | 1.05E-53 | -1.69E-46 |
| MS.gene060569 | MS.gene32274 | 0.810065 | 1.39E-50 | -1.69E-46 |
| MS.gene060804 | MS.gene32274 | 0.855976 | 4.39E-62 | -1.69E-46 |
| MS.gene060818 | MS.gene32274 | 0.875032 | 4.24E-68 | -1.69E-46 |
| MS.gene060835 | MS.gene32274 | 0.801319 | 9.53E-49 | -1.69E-46 |
| MS.gene060837 | MS.gene32274 | 0.835841 | 1.33E-56 | -1.69E-46 |
| MS.gene060981 | MS.gene32274 | 0.802012 | 6.87E-49 | -1.69E-46 |
| MS.gene060986 | MS.gene32274 | 0.802604 | 5.19E-49 | -1.69E-46 |
| MS.gene061041 | MS.gene32274 | 0.864324 | 1.32E-64 | -1.69E-46 |
| MS.gene061049 | MS.gene32274 | 0.872216 | 3.77E-67 | -1.69E-46 |
| MS.gene061051 | MS.gene32274 | -0.863418 | 2.52E-64 | -1.69E-46 |
| MS.gene061154 | MS.gene32274 | 0.817623 | 3.00E-52 | -1.69E-46 |
| MS.gene061159 | MS.gene32274 | 0.813306 | 2.74E-51 | -1.69E-46 |
| MS.gene061200 | MS.gene32274 | 0.838032 | 3.66E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32274.t1 | MTR_8g461390 | 95.595 | 227 | 10 | 0 | 1 | 227 | 1 | 227 | 3.23e-166 | 457 |
| MS.gene32274.t1 | MTR_4g105470 | 40.556 | 180 | 98 | 4 | 39 | 215 | 23 | 196 | 2.76e-31 | 114 |
| MS.gene32274.t1 | MTR_8g069755 | 34.135 | 208 | 123 | 5 | 13 | 215 | 5 | 203 | 1.07e-29 | 110 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32274.t1 | AT1G17100 | 62.632 | 190 | 68 | 1 | 35 | 221 | 42 | 231 | 1.86e-89 | 263 |
| MS.gene32274.t1 | AT1G78450 | 48.214 | 224 | 103 | 4 | 4 | 222 | 1 | 216 | 1.88e-69 | 212 |
| MS.gene32274.t1 | AT1G78460 | 43.316 | 187 | 98 | 3 | 35 | 220 | 40 | 219 | 1.69e-47 | 156 |
Find 45 sgRNAs with CRISPR-Local
Find 89 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTATCTCAATTTCATTATC+AGG | 0.236313 | 8.3:-48881475 | MS.gene32274:CDS |
| GACGTTATAATTCGTCTGTT+TGG | 0.282437 | 8.3:-48881366 | MS.gene32274:CDS |
| TAGGCTATTTGATTACATTC+AGG | 0.303343 | 8.3:-48880295 | MS.gene32274:CDS |
| TTCTTGTTTGATCTGGAAAA+TGG | 0.306508 | 8.3:-48879895 | MS.gene32274:CDS |
| GGATTTGCTTGGTTCACTTT+TGG | 0.319811 | 8.3:+48880162 | None:intergenic |
| TGAACATGAAGACCCTTTGC+AGG | 0.347285 | 8.3:+48880135 | None:intergenic |
| AATGTCTTGAATAGGAGAAT+TGG | 0.347748 | 8.3:+48881339 | None:intergenic |
| CTTGTTGAAGCTACTAGAAC+TGG | 0.386778 | 8.3:-48881313 | MS.gene32274:CDS |
| ACAAATGAAGACTCACAAAA+TGG | 0.387846 | 8.3:+48880201 | None:intergenic |
| ACCCTATTATCGTATTCAAA+AGG | 0.389319 | 8.3:+48879931 | None:intergenic |
| AGCTTCTATGACATCATAGT+TGG | 0.390370 | 8.3:+48881408 | None:intergenic |
| CTGAAGCATGACCAGCTCTA+TGG | 0.399888 | 8.3:+48879980 | None:intergenic |
| ACAAGAGAAATGTCTTGAAT+AGG | 0.403479 | 8.3:+48881331 | None:intergenic |
| GATATGGTTCTTGTTTGATC+TGG | 0.407224 | 8.3:-48879902 | MS.gene32274:CDS |
| CCCTATTATCGTATTCAAAA+GGG | 0.409052 | 8.3:+48879932 | None:intergenic |
| AGGCTATTTGATTACATTCA+GGG | 0.450840 | 8.3:-48880294 | MS.gene32274:CDS |
| TGAAGACCCTTTGCAGGTGG+TGG | 0.452104 | 8.3:+48880141 | None:intergenic |
| TTGCAGGTGGTGGATTTGCT+TGG | 0.453762 | 8.3:+48880151 | None:intergenic |
| AACTATGATGTCATAGAAGC+TGG | 0.463929 | 8.3:-48881406 | MS.gene32274:CDS |
| GCGTTGAAGGACAGTATTGC+CGG | 0.472222 | 8.3:-48880033 | MS.gene32274:CDS |
| ATAATAGGGTGAATGAGATA+TGG | 0.475621 | 8.3:-48879918 | MS.gene32274:CDS |
| TGAGCTAGCATCAGCGTTGA+AGG | 0.486594 | 8.3:-48880046 | MS.gene32274:CDS |
| GATGTCATAGAAGCTGGAAA+TGG | 0.490076 | 8.3:-48881400 | MS.gene32274:CDS |
| AGCAAATCCACCACCTGCAA+AGG | 0.503511 | 8.3:-48880148 | MS.gene32274:CDS |
| TATGCAGCAGTTAAACAGTT+TGG | 0.507098 | 8.3:-48880096 | MS.gene32274:CDS |
| AGGGTCTTCATGTTCAAAGA+TGG | 0.509198 | 8.3:-48880128 | MS.gene32274:CDS |
| GCAGCAGTTAAACAGTTTGG+TGG | 0.512495 | 8.3:-48880093 | MS.gene32274:CDS |
| GGACACTCTATGCGTTTGCA+TGG | 0.518091 | 8.3:+48881430 | None:intergenic |
| TGTTGTGGTGGTTACAGCCA+TGG | 0.533600 | 8.3:+48881522 | None:intergenic |
| GGTAAGACTTCAGAGAGAAC+AGG | 0.536541 | 8.3:+48880234 | None:intergenic |
| AACTCCAAGCATGCAATCCA+TGG | 0.545237 | 8.3:-48881539 | None:intergenic |
| GCAAATCCACCACCTGCAAA+GGG | 0.549447 | 8.3:-48880147 | MS.gene32274:CDS |
| GCTTCTATGACATCATAGTT+GGG | 0.553146 | 8.3:+48881409 | None:intergenic |
| TCACAAAATGGTCCATCACT+TGG | 0.562399 | 8.3:+48880213 | None:intergenic |
| TCTGAAGTCTTACCAAGTGA+TGG | 0.564116 | 8.3:-48880225 | MS.gene32274:CDS |
| GGCTATTTGATTACATTCAG+GGG | 0.574090 | 8.3:-48880293 | MS.gene32274:CDS |
| AGCAGAAGACCACTTAGTAC+CGG | 0.588835 | 8.3:+48880014 | None:intergenic |
| ATTGAACAAAGCCATAGAGC+TGG | 0.606780 | 8.3:-48879991 | MS.gene32274:CDS |
| TTTGTCAAAGATACAGATGT+TGG | 0.611111 | 8.3:-48880069 | MS.gene32274:CDS |
| ACAGTATTGCCGGTACTAAG+TGG | 0.612734 | 8.3:-48880023 | MS.gene32274:CDS |
| CAGTGAGAGCTTCCATGTTG+TGG | 0.619405 | 8.3:+48881507 | None:intergenic |
| CCTATTATCGTATTCAAAAG+GGG | 0.621839 | 8.3:+48879933 | None:intergenic |
| TGGCTGTAACCACCACAACA+TGG | 0.629806 | 8.3:-48881519 | MS.gene32274:CDS |
| TGAGAGCTTCCATGTTGTGG+TGG | 0.652258 | 8.3:+48881510 | None:intergenic |
| ACATGAAGACCCTTTGCAGG+TGG | 0.699868 | 8.3:+48880138 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAGTTGAATACATATAAAA+GGG | - | chr8.3:48880372-48880391 | MS.gene32274:intron | 15.0% |
| !! | AATGTGAAATGATTTATACT+TGG | - | chr8.3:48880814-48880833 | MS.gene32274:intron | 20.0% |
| !! | GAAAGTTGAATACATATAAA+AGG | - | chr8.3:48880371-48880390 | MS.gene32274:intron | 20.0% |
| !! | GTTTATGATACAAAAATATG+AGG | - | chr8.3:48880229-48880248 | MS.gene32274:CDS | 20.0% |
| !!! | AAATTAATCATCTCTAGAAT+TGG | + | chr8.3:48880891-48880910 | None:intergenic | 20.0% |
| !!! | TCAATCTTTTTCTTTTTCTT+AGG | - | chr8.3:48881094-48881113 | MS.gene32274:intron | 20.0% |
| ! | AACTAATTCTAACTTGAAGT+TGG | + | chr8.3:48880606-48880625 | None:intergenic | 25.0% |
| ! | ATGCTAAAATCAATTGTTTC+TGG | - | chr8.3:48880930-48880949 | MS.gene32274:intron | 25.0% |
| ! | CTTATCTCAATTTCATTATC+AGG | - | chr8.3:48879933-48879952 | MS.gene32274:CDS | 25.0% |
| ! | TGAACTTATTGATCATTCAT+TGG | + | chr8.3:48881000-48881019 | None:intergenic | 25.0% |
| ! | TTTAGTGTAATTACAAGTGT+CGG | - | chr8.3:48880506-48880525 | MS.gene32274:intron | 25.0% |
| ! | TTTCAAATTATTAGCTGTGT+CGG | + | chr8.3:48880474-48880493 | None:intergenic | 25.0% |
| !!! | AAGTTTTAATGAAGTGTTTG+TGG | - | chr8.3:48881034-48881053 | MS.gene32274:intron | 25.0% |
| !!! | ACAATTGATTTTAGCATGTT+TGG | + | chr8.3:48880927-48880946 | None:intergenic | 25.0% |
| !!! | GAGTTTTTCAGTAATAGTTT+AGG | - | chr8.3:48880146-48880165 | MS.gene32274:CDS | 25.0% |
| AATGTCTTGAATAGGAGAAT+TGG | + | chr8.3:48880072-48880091 | None:intergenic | 30.0% | |
| ACAAATGAAGACTCACAAAA+TGG | + | chr8.3:48881210-48881229 | None:intergenic | 30.0% | |
| ACAAGAGAAATGTCTTGAAT+AGG | + | chr8.3:48880080-48880099 | None:intergenic | 30.0% | |
| ACCCTATTATCGTATTCAAA+AGG | + | chr8.3:48881480-48881499 | None:intergenic | 30.0% | |
| AGGCTATTTGATTACATTCA+GGG | - | chr8.3:48881114-48881133 | MS.gene32274:intron | 30.0% | |
| ATAATAGGGTGAATGAGATA+TGG | - | chr8.3:48881490-48881509 | MS.gene32274:CDS | 30.0% | |
| CCCTATTATCGTATTCAAAA+GGG | + | chr8.3:48881479-48881498 | None:intergenic | 30.0% | |
| CCTATTATCGTATTCAAAAG+GGG | + | chr8.3:48881478-48881497 | None:intergenic | 30.0% | |
| GATCAAGTTTAGTAACTCTT+CGG | + | chr8.3:48881076-48881095 | None:intergenic | 30.0% | |
| TAGGCTATTTGATTACATTC+AGG | - | chr8.3:48881113-48881132 | MS.gene32274:intron | 30.0% | |
| TAGTGTGATTGGAATTGATT+TGG | - | chr8.3:48880298-48880317 | MS.gene32274:intron | 30.0% | |
| TTTGTCAAAGATACAGATGT+TGG | - | chr8.3:48881339-48881358 | MS.gene32274:CDS | 30.0% | |
| ! | CCCTTTTGAATACGATAATA+GGG | - | chr8.3:48881476-48881495 | MS.gene32274:CDS | 30.0% |
| ! | TTCTTGTTTGATCTGGAAAA+TGG | - | chr8.3:48881513-48881532 | MS.gene32274:CDS | 30.0% |
| !! | GAGTTTGATTTTGACATGTT+TGG | + | chr8.3:48880661-48880680 | None:intergenic | 30.0% |
| AACTATGATGTCATAGAAGC+TGG | - | chr8.3:48880002-48880021 | MS.gene32274:CDS | 35.0% | |
| AAGTTTAGTAACTCTTCGGA+TGG | + | chr8.3:48881072-48881091 | None:intergenic | 35.0% | |
| AGCTTCTATGACATCATAGT+TGG | + | chr8.3:48880003-48880022 | None:intergenic | 35.0% | |
| AGTTTAGTAACTCTTCGGAT+GGG | + | chr8.3:48881071-48881090 | None:intergenic | 35.0% | |
| GACGTTATAATTCGTCTGTT+TGG | - | chr8.3:48880042-48880061 | MS.gene32274:CDS | 35.0% | |
| GCTTCTATGACATCATAGTT+GGG | + | chr8.3:48880002-48880021 | None:intergenic | 35.0% | |
| GGCTATTTGATTACATTCAG+GGG | - | chr8.3:48881115-48881134 | MS.gene32274:intron | 35.0% | |
| TATGCAGCAGTTAAACAGTT+TGG | - | chr8.3:48881312-48881331 | MS.gene32274:CDS | 35.0% | |
| ! | CCCCTTTTGAATACGATAAT+AGG | - | chr8.3:48881475-48881494 | MS.gene32274:CDS | 35.0% |
| ! | GATATGGTTCTTGTTTGATC+TGG | - | chr8.3:48881506-48881525 | MS.gene32274:CDS | 35.0% |
| ! | TTAGTGTGTGTTTGGTTTCA+CGG | + | chr8.3:48880976-48880995 | None:intergenic | 35.0% |
| !! | TCATTGGTTTAGTGTGTGTT+TGG | + | chr8.3:48880984-48881003 | None:intergenic | 35.0% |
| !!! | CTACTAGAACTGGTTTTTTG+AGG | - | chr8.3:48880105-48880124 | MS.gene32274:CDS | 35.0% |
| !!! | CTGTCATCTCAATTTTTTGC+TGG | + | chr8.3:48881152-48881171 | None:intergenic | 35.0% |
| AGGGTCTTCATGTTCAAAGA+TGG | - | chr8.3:48881280-48881299 | MS.gene32274:intron | 40.0% | |
| ATTGAACAAAGCCATAGAGC+TGG | - | chr8.3:48881417-48881436 | MS.gene32274:CDS | 40.0% | |
| CTTGTTGAAGCTACTAGAAC+TGG | - | chr8.3:48880095-48880114 | MS.gene32274:CDS | 40.0% | |
| GATGTCATAGAAGCTGGAAA+TGG | - | chr8.3:48880008-48880027 | MS.gene32274:CDS | 40.0% | |
| GGGAGTTGAAAAATCATGTC+TGG | - | chr8.3:48880392-48880411 | MS.gene32274:intron | 40.0% | |
| GTGTTTGCTTCTAGTGTGAT+TGG | - | chr8.3:48880287-48880306 | MS.gene32274:CDS | 40.0% | |
| TCACAAAATGGTCCATCACT+TGG | + | chr8.3:48881198-48881217 | None:intergenic | 40.0% | |
| ! | TAATGAAGTGTTTGTGGTGC+TGG | - | chr8.3:48881040-48881059 | MS.gene32274:intron | 40.0% |
| ! | TCTGAAGTCTTACCAAGTGA+TGG | - | chr8.3:48881183-48881202 | MS.gene32274:intron | 40.0% |
| !! | GGATTTGCTTGGTTCACTTT+TGG | + | chr8.3:48881249-48881268 | None:intergenic | 40.0% |
| ACACTTCCCAACGTAAACAG+TGG | + | chr8.3:48880687-48880706 | None:intergenic | 45.0% | |
| ACAGTATTGCCGGTACTAAG+TGG | - | chr8.3:48881385-48881404 | MS.gene32274:CDS | 45.0% | |
| ACTCTTCCCACTGTTTACGT+TGG | - | chr8.3:48880677-48880696 | MS.gene32274:intron | 45.0% | |
| AGCAGAAGACCACTTAGTAC+CGG | + | chr8.3:48881397-48881416 | None:intergenic | 45.0% | |
| CACTTCCCAACGTAAACAGT+GGG | + | chr8.3:48880686-48880705 | None:intergenic | 45.0% | |
| CTCTTCCCACTGTTTACGTT+GGG | - | chr8.3:48880678-48880697 | MS.gene32274:intron | 45.0% | |
| GCAGCAGTTAAACAGTTTGG+TGG | - | chr8.3:48881315-48881334 | MS.gene32274:CDS | 45.0% | |
| GGTAAGACTTCAGAGAGAAC+AGG | + | chr8.3:48881177-48881196 | None:intergenic | 45.0% | |
| TGAACATGAAGACCCTTTGC+AGG | + | chr8.3:48881276-48881295 | None:intergenic | 45.0% | |
| ! | ATGTCTGGAGCTATGAAGCA+CGG | - | chr8.3:48880407-48880426 | MS.gene32274:intron | 45.0% |
| ACATGAAGACCCTTTGCAGG+TGG | + | chr8.3:48881273-48881292 | None:intergenic | 50.0% | |
| AGCAAATCCACCACCTGCAA+AGG | - | chr8.3:48881260-48881279 | MS.gene32274:intron | 50.0% | |
| CAGTGAGAGCTTCCATGTTG+TGG | + | chr8.3:48879904-48879923 | None:intergenic | 50.0% | |
| CTGAAGCATGACCAGCTCTA+TGG | + | chr8.3:48881431-48881450 | None:intergenic | 50.0% | |
| GCAAATCCACCACCTGCAAA+GGG | - | chr8.3:48881261-48881280 | MS.gene32274:intron | 50.0% | |
| GCGTTGAAGGACAGTATTGC+CGG | - | chr8.3:48881375-48881394 | MS.gene32274:CDS | 50.0% | |
| GCTGGGATACGTCTAATTCG+AGG | - | chr8.3:48880538-48880557 | MS.gene32274:intron | 50.0% | |
| GGACACTCTATGCGTTTGCA+TGG | + | chr8.3:48879981-48880000 | None:intergenic | 50.0% | |
| GTGTCTGTGCTTCATAGGTC+TGG | - | chr8.3:48880562-48880581 | MS.gene32274:intron | 50.0% | |
| TCTGTGCTTCATAGGTCTGG+AGG | - | chr8.3:48880565-48880584 | MS.gene32274:intron | 50.0% | |
| TGAGAGCTTCCATGTTGTGG+TGG | + | chr8.3:48879901-48879920 | None:intergenic | 50.0% | |
| TGGCTGTAACCACCACAACA+TGG | - | chr8.3:48879889-48879908 | MS.gene32274:CDS | 50.0% | |
| TGTTGTGGTGGTTACAGCCA+TGG | + | chr8.3:48879889-48879908 | None:intergenic | 50.0% | |
| ! | GTGTGTGTTTGGTTTCACGG+TGG | + | chr8.3:48880973-48880992 | None:intergenic | 50.0% |
| ! | TGAGCTAGCATCAGCGTTGA+AGG | - | chr8.3:48881362-48881381 | MS.gene32274:CDS | 50.0% |
| !! | GAGGAGTGTCTGTGCTTCAT+AGG | - | chr8.3:48880557-48880576 | MS.gene32274:intron | 50.0% |
| !! | TTGCAGGTGGTGGATTTGCT+TGG | + | chr8.3:48881260-48881279 | None:intergenic | 50.0% |
| ATGAAGCACGGATGCGGACA+CGG | - | chr8.3:48880419-48880438 | MS.gene32274:intron | 55.0% | |
| GGAGCTATGAAGCACGGATG+CGG | - | chr8.3:48880413-48880432 | MS.gene32274:intron | 55.0% | |
| TGAAGACCCTTTGCAGGTGG+TGG | + | chr8.3:48881270-48881289 | None:intergenic | 55.0% | |
| GTCAGTGTCCGTGTCGTGTC+CGG | + | chr8.3:48880448-48880467 | None:intergenic | 60.0% | |
| !! | AAGTGTCGGTGTCGTGTCGC+TGG | - | chr8.3:48880520-48880539 | MS.gene32274:intron | 60.0% |
| !! | AGTGTCGGTGTCGTGTCGCT+GGG | - | chr8.3:48880521-48880540 | MS.gene32274:intron | 60.0% |
| ACGGATGCGGACACGGACAC+CGG | - | chr8.3:48880426-48880445 | MS.gene32274:intron | 65.0% | |
| CACGGACACCGGACACGACA+CGG | - | chr8.3:48880437-48880456 | MS.gene32274:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 48879879 | 48881551 | 48879879 | ID=MS.gene32274 |
| chr8.3 | mRNA | 48879879 | 48881551 | 48879879 | ID=MS.gene32274.t1;Parent=MS.gene32274 |
| chr8.3 | exon | 48881304 | 48881551 | 48881304 | ID=MS.gene32274.t1.exon1;Parent=MS.gene32274.t1 |
| chr8.3 | CDS | 48881304 | 48881551 | 48881304 | ID=cds.MS.gene32274.t1;Parent=MS.gene32274.t1 |
| chr8.3 | exon | 48879879 | 48880314 | 48879879 | ID=MS.gene32274.t1.exon2;Parent=MS.gene32274.t1 |
| chr8.3 | CDS | 48879879 | 48880314 | 48879879 | ID=cds.MS.gene32274.t1;Parent=MS.gene32274.t1 |
| Gene Sequence |
| Protein sequence |
