AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene33004


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33004.t1 MTR_1g016170 95.575 226 10 0 1 226 80 305 1.40e-137 388
MS.gene33004.t1 MTR_3g050550 82.979 94 16 0 18 111 1 94 7.87e-49 155
MS.gene33004.t1 MTR_3g050610 76.667 120 23 2 4 121 31 147 3.41e-44 145
MS.gene33004.t1 MTR_4g126350 45.872 109 38 5 29 137 128 215 6.11e-18 81.3
MS.gene33004.t1 MTR_2g038720 37.850 214 80 8 29 224 110 288 1.34e-16 77.0
MS.gene33004.t1 MTR_4g121110 62.500 48 18 0 105 152 27 74 4.94e-16 72.8
MS.gene33004.t1 MTR_7g112330 69.444 36 11 0 108 143 22 57 4.26e-13 64.3
MS.gene33004.t1 MTR_5g020230 57.692 52 19 1 96 144 107 158 2.73e-12 64.3
MS.gene33004.t1 MTR_1g094930 56.098 41 18 0 103 143 142 182 3.03e-11 61.6
MS.gene33004.t1 MTR_1g094930 56.098 41 18 0 103 143 237 277 4.80e-11 61.6
MS.gene33004.t1 MTR_8g077510 63.158 38 14 0 107 144 124 161 6.05e-11 60.8
MS.gene33004.t1 MTR_1g116010 33.721 86 46 1 58 143 125 199 7.89e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33004.t1 AT5G56860 41.985 131 49 6 29 141 142 263 7.32e-20 87.4
MS.gene33004.t1 AT5G49300 66.667 45 15 0 104 148 32 76 2.62e-17 75.9
MS.gene33004.t1 AT4G26150 43.220 118 26 4 29 139 147 230 2.02e-16 77.4
MS.gene33004.t1 AT5G26930 66.667 42 14 0 104 145 22 63 3.70e-16 72.4
MS.gene33004.t1 AT3G06740 66.667 42 14 0 105 146 38 79 1.02e-14 69.3
MS.gene33004.t1 AT4G16141 68.293 41 13 0 105 145 34 74 1.69e-14 69.7
MS.gene33004.t1 AT3G16870 57.143 56 20 1 105 160 39 90 3.16e-14 68.9
MS.gene33004.t1 AT4G36620 58.333 48 20 0 104 151 71 118 1.76e-12 64.7
MS.gene33004.t1 AT2G18380 68.293 41 12 1 108 147 92 132 3.62e-12 63.5
MS.gene33004.t1 AT5G25830 55.556 45 20 0 99 143 210 254 1.53e-11 63.2
MS.gene33004.t1 AT3G50870 33.058 121 68 4 107 224 151 261 2.49e-11 62.4
MS.gene33004.t1 AT1G08010 57.778 45 18 1 99 143 212 255 5.11e-11 61.6
MS.gene33004.t1 AT1G08010 57.778 45 18 1 99 143 212 255 5.11e-11 61.6
MS.gene33004.t1 AT1G08010 57.778 45 18 1 99 143 212 255 5.11e-11 61.6
MS.gene33004.t1 AT2G45050 46.000 50 27 0 94 143 165 214 7.79e-11 60.8

Find 53 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTTGTTTGGTCTCATTAT+TGG 0.223553 1.1:-5994558 None:intergenic
GGACTAGTACCATAATTAAT+AGG 0.299944 1.1:-5994633 None:intergenic
ATGATGAAACCTATTAATTA+TGG 0.328213 1.1:+5994624 MS.gene33004:CDS
TTGTTACCGCTGTTACTACT+AGG 0.336848 1.1:-5994690 None:intergenic
TGTTACTACTAGGGCTTGTT+TGG 0.337618 1.1:-5994680 None:intergenic
CACTCCTCCATAGTGGGGTA+TGG 0.338618 1.1:-5994746 None:intergenic
GTCCAAGAAATCAAAATATA+AGG 0.340331 1.1:+5994652 MS.gene33004:CDS
TTTGCAATGCTTGTGGAATC+AGG 0.357272 1.1:+5995368 MS.gene33004:CDS
TCATCATAATCATAATCTAA+TGG 0.373491 1.1:-5995580 None:intergenic
TAATTAATAGGTTTCATCAT+TGG 0.391140 1.1:-5994621 None:intergenic
CACAAATTTATCATCATCCT+TGG 0.395360 1.1:+5994425 MS.gene33004:CDS
AGCAACGGCGGCACGACTTC+TGG 0.401059 1.1:+5995606 MS.gene33004:CDS
GTGGCTGCAATATTGTTGTT+TGG 0.421269 1.1:-5994570 None:intergenic
GGCTGAAGCTGCCAATGGTT+TGG 0.422143 1.1:+5995414 MS.gene33004:CDS
CGAATTACCTTTGGACCCAT+AGG 0.444966 1.1:-5994771 None:intergenic
ACTATGGAGGAGTGGCCCTA+TGG 0.450797 1.1:+5994755 MS.gene33004:CDS
TGAGTCACGGGTCTGAGAAA+TGG 0.451328 1.1:+5994472 MS.gene33004:CDS
CTTCTGGTGGATATATCTTC+TGG 0.451597 1.1:+5995622 MS.gene33004:CDS
ACAGCGGTAACAACACCGTT+AGG 0.456940 1.1:+5994700 MS.gene33004:CDS
CTATGGAGGAGTGGCCCTAT+GGG 0.464867 1.1:+5994756 MS.gene33004:CDS
TTTATCATCATCCTTGGAGC+TGG 0.479939 1.1:+5994431 MS.gene33004:CDS
CTTGATAGCTTTGTCAGTAG+TGG 0.482210 1.1:-5994589 None:intergenic
ACTCCTCCATAGTGGGGTAT+GGG 0.482841 1.1:-5994745 None:intergenic
GTACATCCCATACCCCACTA+TGG 0.488777 1.1:+5994739 MS.gene33004:CDS
TTCTTAGTCTTGAATTGTGT+AGG 0.495944 1.1:-5995475 None:intergenic
GCTGCTATCTTCCAGCTCCA+AGG 0.513483 1.1:-5994442 None:intergenic
TGGTCTCATTATTGGAGTTG+TGG 0.515285 1.1:-5994550 None:intergenic
TCAGGCAGAGAAAGGCAAGA+AGG 0.520139 1.1:+5995386 MS.gene33004:CDS
ACAACTTCAACTACTTCTGC+TGG 0.524955 1.1:+5995520 MS.gene33004:CDS
CAGGCAGAGAAAGGCAAGAA+GGG 0.526577 1.1:+5995387 MS.gene33004:CDS
GAATTACCTTTGGACCCATA+GGG 0.528192 1.1:-5994770 None:intergenic
AGGGGATGTAGCCAAACCAT+TGG 0.539225 1.1:-5995425 None:intergenic
CAGTCACTTTGCAATGCTTG+TGG 0.543553 1.1:+5995361 MS.gene33004:intron
CAGCGGTAACAACACCGTTA+GGG 0.546032 1.1:+5994701 MS.gene33004:CDS
ACCATTGGCAGCTTCAGCCA+TGG 0.551217 1.1:-5995410 None:intergenic
TTGTGGAATCAGGCAGAGAA+AGG 0.551715 1.1:+5995378 MS.gene33004:CDS
TAGGGCCACTCCTCCATAGT+GGG 0.558056 1.1:-5994752 None:intergenic
TGTTACCGCTGTTACTACTA+GGG 0.563428 1.1:-5994689 None:intergenic
ATAGGGCCACTCCTCCATAG+TGG 0.566452 1.1:-5994753 None:intergenic
AGAAGTAGTTGAAGTTGTAG+AGG 0.579757 1.1:-5995515 None:intergenic
GAGAAAGGCAAGAAGGGCCA+TGG 0.585866 1.1:+5995393 MS.gene33004:CDS
GAGTGGCCCTATGGGTCCAA+AGG 0.590272 1.1:+5994764 MS.gene33004:CDS
TGATTATGATGAAGCAGCAA+CGG 0.614524 1.1:+5995591 MS.gene33004:CDS
GCCATGGCTGAAGCTGCCAA+TGG 0.619219 1.1:+5995409 MS.gene33004:CDS
AATGGATGTCTTCAAAGATG+AGG 0.632702 1.1:+5994490 MS.gene33004:CDS
AACGGCGGCACGACTTCTGG+TGG 0.640817 1.1:+5995609 MS.gene33004:CDS
ACAATCAGAACAAACCCTAA+CGG 0.645452 1.1:-5994715 None:intergenic
ACAATTCAAGACTAAGAACA+AGG 0.668290 1.1:+5995480 MS.gene33004:CDS
CATACCCCACTATGGAGGAG+TGG 0.671274 1.1:+5994747 MS.gene33004:CDS
CATCCCATACCCCACTATGG+AGG 0.682705 1.1:+5994742 MS.gene33004:CDS
TTATGATGAAGCAGCAACGG+CGG 0.694941 1.1:+5995594 MS.gene33004:CDS
AGGGCCACTCCTCCATAGTG+GGG 0.711722 1.1:-5994751 None:intergenic
ACAAGCCCTAGTAGTAACAG+CGG 0.724459 1.1:+5994684 MS.gene33004:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATCAAATTAAGTTTAATTA+TGG - chr1.1:5995289-5995308 None:intergenic 10.0%
!! ATTAATATTTATTGTTCATA+AGG + chr1.1:5994934-5994953 MS.gene33004:intron 10.0%
!! TAATTAAAAAGATAAAATAG+AGG - chr1.1:5995118-5995137 None:intergenic 10.0%
!! TGATAAAATATTTGTATTAA+AGG - chr1.1:5995021-5995040 None:intergenic 10.0%
!! TTAATATTTATTGTTCATAA+GGG + chr1.1:5994935-5994954 MS.gene33004:intron 10.0%
!!! ATTAATATATTTTTTTTGTG+GGG - chr1.1:5994920-5994939 None:intergenic 10.0%
!!! GATATTAATAATTTTTGAGA+TGG - chr1.1:5994895-5994914 None:intergenic 15.0%
!!! TGTTTTTGTAAGAATTTTTA+TGG - chr1.1:5994987-5995006 None:intergenic 15.0%
!! ATGATGAAACCTATTAATTA+TGG + chr1.1:5994624-5994643 MS.gene33004:CDS 20.0%
!! ATTTGTATTAAAGGAAGTAA+AGG - chr1.1:5995012-5995031 None:intergenic 20.0%
!! TAATTAATAGGTTTCATCAT+TGG - chr1.1:5994624-5994643 None:intergenic 20.0%
!! TCATCATAATCATAATCTAA+TGG - chr1.1:5995583-5995602 None:intergenic 20.0%
!!! CGATAAATGATAAATTTTAG+TGG - chr1.1:5994861-5994880 None:intergenic 20.0%
!!! TACCTTATATTTTGATTTCT+TGG - chr1.1:5994657-5994676 None:intergenic 20.0%
! ACTATTCTTTCGTATTAAAG+TGG + chr1.1:5994812-5994831 MS.gene33004:intron 25.0%
! ATACGAAAGAATAGTGAATA+AGG - chr1.1:5994807-5994826 None:intergenic 25.0%
! CTATTCTTTCGTATTAAAGT+GGG + chr1.1:5994813-5994832 MS.gene33004:intron 25.0%
! GTCCAAGAAATCAAAATATA+AGG + chr1.1:5994652-5994671 MS.gene33004:CDS 25.0%
! TAAAAAGATAAAATAGAGGC+AGG - chr1.1:5995114-5995133 None:intergenic 25.0%
! TAGTTAACATGAAAACATGT+TGG + chr1.1:5995336-5995355 MS.gene33004:intron 25.0%
!!! ATCTTAAGCACTTTAGTTTT+AGG - chr1.1:5995448-5995467 None:intergenic 25.0%
!!! TCTTAAGCACTTTAGTTTTA+GGG - chr1.1:5995447-5995466 None:intergenic 25.0%
AAAGAATAGTGAATAAGGCT+GGG - chr1.1:5994802-5994821 None:intergenic 30.0%
ACAATTCAAGACTAAGAACA+AGG + chr1.1:5995480-5995499 MS.gene33004:CDS 30.0%
CACAAATTTATCATCATCCT+TGG + chr1.1:5994425-5994444 MS.gene33004:CDS 30.0%
TTCATAAGGGATGTCAAATA+TGG + chr1.1:5994948-5994967 MS.gene33004:intron 30.0%
! GGACTAGTACCATAATTAAT+AGG - chr1.1:5994636-5994655 None:intergenic 30.0%
! TTTAGTGGATCATAACAACA+AGG - chr1.1:5994846-5994865 None:intergenic 30.0%
!! TTCTTAGTCTTGAATTGTGT+AGG - chr1.1:5995478-5995497 None:intergenic 30.0%
!! TTGTTGTTTGGTCTCATTAT+TGG - chr1.1:5994561-5994580 None:intergenic 30.0%
!!! CTTAAGCACTTTAGTTTTAG+GGG - chr1.1:5995446-5995465 None:intergenic 30.0%
AATGGATGTCTTCAAAGATG+AGG + chr1.1:5994490-5994509 MS.gene33004:CDS 35.0%
ACAATCAGAACAAACCCTAA+CGG - chr1.1:5994718-5994737 None:intergenic 35.0%
AGAAGTAGTTGAAGTTGTAG+AGG - chr1.1:5995518-5995537 None:intergenic 35.0%
GAAAGAATAGTGAATAAGGC+TGG - chr1.1:5994803-5994822 None:intergenic 35.0%
TGATTATGATGAAGCAGCAA+CGG + chr1.1:5995591-5995610 MS.gene33004:CDS 35.0%
TGGGATTAACGAATTACCTT+TGG - chr1.1:5994783-5994802 None:intergenic 35.0%
ACAACTTCAACTACTTCTGC+TGG + chr1.1:5995520-5995539 MS.gene33004:CDS 40.0%
AGCAGCAAAAAGATGAGTCA+CGG + chr1.1:5994459-5994478 MS.gene33004:CDS 40.0%
GAATTACCTTTGGACCCATA+GGG - chr1.1:5994773-5994792 None:intergenic 40.0%
TGGTCTCATTATTGGAGTTG+TGG - chr1.1:5994553-5994572 None:intergenic 40.0%
TGTTACCGCTGTTACTACTA+GGG - chr1.1:5994692-5994711 None:intergenic 40.0%
TTGTTACCGCTGTTACTACT+AGG - chr1.1:5994693-5994712 None:intergenic 40.0%
TTTATCATCATCCTTGGAGC+TGG + chr1.1:5994431-5994450 MS.gene33004:CDS 40.0%
TTTGCAATGCTTGTGGAATC+AGG + chr1.1:5995368-5995387 MS.gene33004:CDS 40.0%
! CTTCTGGTGGATATATCTTC+TGG + chr1.1:5995622-5995641 MS.gene33004:CDS 40.0%
! GTGGCTGCAATATTGTTGTT+TGG - chr1.1:5994573-5994592 None:intergenic 40.0%
! TGTTACTACTAGGGCTTGTT+TGG - chr1.1:5994683-5994702 None:intergenic 40.0%
!! CTTGATAGCTTTGTCAGTAG+TGG - chr1.1:5994592-5994611 None:intergenic 40.0%
ACAAGCCCTAGTAGTAACAG+CGG + chr1.1:5994684-5994703 MS.gene33004:CDS 45.0%
CGAATTACCTTTGGACCCAT+AGG - chr1.1:5994774-5994793 None:intergenic 45.0%
GCAGCAAAAAGATGAGTCAC+GGG + chr1.1:5994460-5994479 MS.gene33004:CDS 45.0%
TTATGATGAAGCAGCAACGG+CGG + chr1.1:5995594-5995613 MS.gene33004:CDS 45.0%
TTGTGGAATCAGGCAGAGAA+AGG + chr1.1:5995378-5995397 MS.gene33004:CDS 45.0%
! CAGTCACTTTGCAATGCTTG+TGG + chr1.1:5995361-5995380 MS.gene33004:intron 45.0%
!!! ATATTAATATATTTTTTTTG+TGG - chr1.1:5994922-5994941 None:intergenic 5.0%
!!! TAATTTTTATTATTATATCA+CGG + chr1.1:5995202-5995221 MS.gene33004:intron 5.0%
!!! TATTAATATATTTTTTTTGT+GGG - chr1.1:5994921-5994940 None:intergenic 5.0%
ACAGCGGTAACAACACCGTT+AGG + chr1.1:5994700-5994719 MS.gene33004:CDS 50.0%
AGGGGATGTAGCCAAACCAT+TGG - chr1.1:5995428-5995447 None:intergenic 50.0%
CAGCGGTAACAACACCGTTA+GGG + chr1.1:5994701-5994720 MS.gene33004:CDS 50.0%
CAGGCAGAGAAAGGCAAGAA+GGG + chr1.1:5995387-5995406 MS.gene33004:CDS 50.0%
GTACATCCCATACCCCACTA+TGG + chr1.1:5994739-5994758 MS.gene33004:CDS 50.0%
TCAGGCAGAGAAAGGCAAGA+AGG + chr1.1:5995386-5995405 MS.gene33004:CDS 50.0%
TGAGTCACGGGTCTGAGAAA+TGG + chr1.1:5994472-5994491 MS.gene33004:CDS 50.0%
! ACTCCTCCATAGTGGGGTAT+GGG - chr1.1:5994748-5994767 None:intergenic 50.0%
ACCATTGGCAGCTTCAGCCA+TGG - chr1.1:5995413-5995432 None:intergenic 55.0%
ACTATGGAGGAGTGGCCCTA+TGG + chr1.1:5994755-5994774 MS.gene33004:CDS 55.0%
ATAGGGCCACTCCTCCATAG+TGG - chr1.1:5994756-5994775 None:intergenic 55.0%
CATACCCCACTATGGAGGAG+TGG + chr1.1:5994747-5994766 MS.gene33004:CDS 55.0%
CATCCCATACCCCACTATGG+AGG + chr1.1:5994742-5994761 MS.gene33004:CDS 55.0%
CTATGGAGGAGTGGCCCTAT+GGG + chr1.1:5994756-5994775 MS.gene33004:CDS 55.0%
GAGAAAGGCAAGAAGGGCCA+TGG + chr1.1:5995393-5995412 MS.gene33004:CDS 55.0%
GCTGCTATCTTCCAGCTCCA+AGG - chr1.1:5994445-5994464 None:intergenic 55.0%
GGCTGAAGCTGCCAATGGTT+TGG + chr1.1:5995414-5995433 MS.gene33004:CDS 55.0%
TAGGGCCACTCCTCCATAGT+GGG - chr1.1:5994755-5994774 None:intergenic 55.0%
! CACTCCTCCATAGTGGGGTA+TGG - chr1.1:5994749-5994768 None:intergenic 55.0%
AGGGCCACTCCTCCATAGTG+GGG - chr1.1:5994754-5994773 None:intergenic 60.0%
GAGTGGCCCTATGGGTCCAA+AGG + chr1.1:5994764-5994783 MS.gene33004:CDS 60.0%
GCCATGGCTGAAGCTGCCAA+TGG + chr1.1:5995409-5995428 MS.gene33004:CDS 60.0%
AACGGCGGCACGACTTCTGG+TGG + chr1.1:5995609-5995628 MS.gene33004:CDS 65.0%
AGCAACGGCGGCACGACTTC+TGG + chr1.1:5995606-5995625 MS.gene33004:CDS 65.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 5994399 5995657 5994399 ID=MS.gene33004
chr1.1 mRNA 5994399 5995657 5994399 ID=MS.gene33004.t1;Parent=MS.gene33004
chr1.1 exon 5994399 5994785 5994399 ID=MS.gene33004.t1.exon1;Parent=MS.gene33004.t1
chr1.1 CDS 5994399 5994785 5994399 ID=cds.MS.gene33004.t1;Parent=MS.gene33004.t1
chr1.1 exon 5995364 5995657 5995364 ID=MS.gene33004.t1.exon2;Parent=MS.gene33004.t1
chr1.1 CDS 5995364 5995657 5995364 ID=cds.MS.gene33004.t1;Parent=MS.gene33004.t1
Gene Sequence

>MS.gene33004

ATGGTTGATTCAAGCAGTGCACGTGACACAAATTTATCATCATCCTTGGAGCTGGAAGATAGCAGCAAAAAGATGAGTCACGGGTCTGAGAAATGGATGTCTTCAAAGATGAGGTTGATGAATAAAATGATCAACACAACTGCTACTGTTGCCACAACTCCAATAATGAGACCAAACAACAATATTGCAGCCACTACTGACAAAGCTATCAAGACAACAACTCCAATGATGAAACCTATTAATTATGGTACTAGTCCAAGAAATCAAAATATAAGGTACAACCAAACAAGCCCTAGTAGTAACAGCGGTAACAACACCGTTAGGGTTTGTTCTGATTGTAGTACATCCCATACCCCACTATGGAGGAGTGGCCCTATGGGTCCAAAGGTAATTCGTTAATCCCAGCCTTATTCACTATTCTTTCGTATTAAAGTGGGAGAGTGACCTTGTTGTTATGATCCACTAAAATTTATCATTTATCGCTTCAATTCGTCCATCTCAAAAATTATTAATATCATCCCCACAAAAAAAATATATTAATATTTATTGTTCATAAGGGATGTCAAATATGGATTTTTATCAAGACCATAAAAATTCTTACAAAAACAATCCTTTACTTCCTTTAATACAAATATTTTATCAAAAAAATGTAGTATTCGTAGTAAAAGTTGAATTCATAGAAACGTCAAAATTAGAGATCAATTTAATTTTTCCTGCCTCTATTTTATCTTTTTAATTACAATATTTATAGTTGATATTCAAACATAAATAAGATATCAAGCTATATACAAGATCAATTAAAATAATTTTTATTATTATATCACGGTTTTAATTAGTCGAATATTTCAATAGATGATAGATCACACTAGAGTCATGCAAGTATTATTCCATAATTAAACTTAATTTGATTGATACTTGAATCATATCTAGTTATATATAGTTAACATGAAAACATGTTGGTGCAGTCACTTTGCAATGCTTGTGGAATCAGGCAGAGAAAGGCAAGAAGGGCCATGGCTGAAGCTGCCAATGGTTTGGCTACATCCCCTAAAACTAAAGTGCTTAAGATAAAGAAGCCTACACAATTCAAGACTAAGAACAAGGCTTCTACTAGTACCTCTACAACTTCAACTACTTCTGCTGGCTCATCATCTCAAGATGTGAAGAAACTTGAATCTTTTCCATTAGATTATGATTATGATGAAGCAGCAACGGCGGCACGACTTCTGGTGGATATATCTTCTGGTGTTACTTATTGA

Protein sequence

>MS.gene33004.t1

MVDSSSARDTNLSSSLELEDSSKKMSHGSEKWMSSKMRLMNKMINTTATVATTPIMRPNNNIAATTDKAIKTTTPMMKPINYGTSPRNQNIRYNQTSPSSNSGNNTVRVCSDCSTSHTPLWRSGPMGPKSLCNACGIRQRKARRAMAEAANGLATSPKTKVLKIKKPTQFKTKNKASTSTSTTSTTSAGSSSQDVKKLESFPLDYDYDEAATAARLLVDISSGVTY