AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene34187


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34187.t1 MTR_6g007180 97.849 93 2 0 1 93 208 300 9.29e-62 189
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34187.t1 AT3G55250 56.044 91 39 1 1 91 185 274 8.19e-24 91.7

Find 15 sgRNAs with CRISPR-Local

Find 16 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAATCTACGTTTGTTGCTT+CGG 0.330000 6.2:+2731483 MS.gene34187:CDS
AGAGATTCGGCGCATGGTTA+AGG 0.388940 6.2:+2731584 MS.gene34187:CDS
TCAACAGCTTCCTTTGATAG+AGG 0.406723 6.2:-2731663 None:intergenic
CTCTCATCTTCTGTCTCTAT+AGG 0.411361 6.2:-2731513 None:intergenic
GATAGAGGCTTAGCAGCATC+TGG 0.416100 6.2:-2731648 None:intergenic
GAAAGTAGAAGACGGAGGTA+TGG 0.445392 6.2:+2731617 MS.gene34187:CDS
TTCTCAACTCGAACCTCCAT+TGG 0.453422 6.2:-2731450 None:intergenic
AAAACAAGAGATTCGGCGCA+TGG 0.513137 6.2:+2731578 MS.gene34187:CDS
CTACGTTTGTTGCTTCGGAA+AGG 0.538343 6.2:+2731488 MS.gene34187:CDS
TCGGCGCATGGTTAAGGAGA+TGG 0.565322 6.2:+2731590 MS.gene34187:CDS
TGCTGCTAAGCCTCTATCAA+AGG 0.573332 6.2:+2731653 MS.gene34187:CDS
AATGGAGGTTCGAGTTGAGA+AGG 0.577559 6.2:+2731452 None:intergenic
CTATAGAGACAGAAGATGAG+AGG 0.617615 6.2:+2731514 MS.gene34187:CDS
ATGGCAGAGAAAGTAGAAGA+CGG 0.643691 6.2:+2731609 MS.gene34187:CDS
GCAGAGAAAGTAGAAGACGG+AGG 0.701413 6.2:+2731612 MS.gene34187:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTTGTTTGAGAAAGTTA+AGG - chr6.2:2731704-2731723 None:intergenic 20.0%
CAGTTCAAAAACAAGAGATT+CGG + chr6.2:2731571-2731590 MS.gene34187:CDS 30.0%
!!! ATCTCTTGTTTTTGAACTGA+TGG - chr6.2:2731570-2731589 None:intergenic 30.0%
ATGGCAGAGAAAGTAGAAGA+CGG + chr6.2:2731609-2731628 MS.gene34187:CDS 40.0%
CTATAGAGACAGAAGATGAG+AGG + chr6.2:2731514-2731533 MS.gene34187:CDS 40.0%
CTCTCATCTTCTGTCTCTAT+AGG - chr6.2:2731516-2731535 None:intergenic 40.0%
TCAACAGCTTCCTTTGATAG+AGG - chr6.2:2731666-2731685 None:intergenic 40.0%
! GCAATCTACGTTTGTTGCTT+CGG + chr6.2:2731483-2731502 MS.gene34187:CDS 40.0%
AAAACAAGAGATTCGGCGCA+TGG + chr6.2:2731578-2731597 MS.gene34187:CDS 45.0%
GAAAGTAGAAGACGGAGGTA+TGG + chr6.2:2731617-2731636 MS.gene34187:CDS 45.0%
TGCTGCTAAGCCTCTATCAA+AGG + chr6.2:2731653-2731672 MS.gene34187:CDS 45.0%
! CTACGTTTGTTGCTTCGGAA+AGG + chr6.2:2731488-2731507 MS.gene34187:CDS 45.0%
AGAGATTCGGCGCATGGTTA+AGG + chr6.2:2731584-2731603 MS.gene34187:CDS 50.0%
GATAGAGGCTTAGCAGCATC+TGG - chr6.2:2731651-2731670 None:intergenic 50.0%
GCAGAGAAAGTAGAAGACGG+AGG + chr6.2:2731612-2731631 MS.gene34187:CDS 50.0%
TCGGCGCATGGTTAAGGAGA+TGG + chr6.2:2731590-2731609 MS.gene34187:CDS 55.0%


Chromosome Type Strat End Strand Name
chr6.2 gene 2731453 2731734 2731453 ID=MS.gene34187
chr6.2 mRNA 2731453 2731734 2731453 ID=MS.gene34187.t1;Parent=MS.gene34187
chr6.2 exon 2731453 2731734 2731453 ID=MS.gene34187.t1.exon1;Parent=MS.gene34187.t1
chr6.2 CDS 2731453 2731734 2731453 ID=cds.MS.gene34187.t1;Parent=MS.gene34187.t1
Gene Sequence

>MS.gene34187

ATGGAGGTTCGAGTTGAGAAGGCTCTTGATGCAATCTACGTTTGTTGCTTCGGAAAGGATCCTATAGAGACAGAAGATGAGAGGTTGCTGAGTACAATACTTAGTTCAGTTTTTCCATCAGTTCAAAAACAAGAGATTCGGCGCATGGTTAAGGAGATGGCAGAGAAAGTAGAAGACGGAGGTATGGATTATATTCCAGATGCTGCTAAGCCTCTATCAAAGGAAGCTGTTGAAATGCAAATGAAAGACCTTAACTTTCTCAAACAAAATAGTAACAATTAG

Protein sequence

>MS.gene34187.t1

MEVRVEKALDAIYVCCFGKDPIETEDERLLSTILSSVFPSVQKQEIRRMVKEMAEKVEDGGMDYIPDAAKPLSKEAVEMQMKDLNFLKQNSNN