AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene34843


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34843.t1 MTR_0004s0440 85.841 226 13 3 1 221 1 212 1.87e-133 374
MS.gene34843.t1 MTR_1g021995 32.000 225 138 5 11 221 37 260 9.28e-32 116
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34843.t1 AT3G47430 73.661 224 54 1 3 221 4 227 1.74e-116 331
MS.gene34843.t1 AT1G47750 29.915 234 147 6 3 221 16 247 5.24e-24 96.3

Find 40 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGAACCTCTTGGATATATC+AGG 0.230932 4.4:+60881527 None:intergenic
CTAGCAGTGCTTTCTAATTC+AGG 0.262558 4.4:-60881392 MS.gene34843:CDS
TTTCTTTGGTTGTCAAGAAT+TGG 0.292472 4.4:-60881341 MS.gene34843:CDS
CACACGCCAGTTTGCAAGCT+TGG 0.301187 4.4:+60881562 None:intergenic
ATGAGTTTCATATCTGCTTT+TGG 0.310972 4.4:-60881290 MS.gene34843:CDS
CTGGTTCTACTCTCACTTTA+AGG 0.312353 4.4:-60881418 MS.gene34843:CDS
TTGCAAGCTTGGAAACATAT+TGG 0.323667 4.4:+60881573 None:intergenic
ACTGTTTCTCTTGGTATTAG+TGG 0.331245 4.4:-60881050 MS.gene34843:CDS
AGAACTGGAAGGTTTCTTAC+TGG 0.334576 4.4:-60881464 MS.gene34843:CDS
TTTGTCCACTGTGTCATTCA+TGG 0.336012 4.4:+60881649 None:intergenic
GGGCTGGTTGGTATAGAAAT+TGG 0.339600 4.4:-60881013 MS.gene34843:CDS
TTCTCTTGGTATTAGTGGAT+TGG 0.370396 4.4:-60881045 MS.gene34843:CDS
TTCTTGACAACCAAAGAAAA+TGG 0.378899 4.4:+60881345 None:intergenic
TGCAATCACACTGTTTCTCT+TGG 0.395141 4.4:-60881059 MS.gene34843:CDS
GCTTTCTAATTCAGGAGAAA+TGG 0.401761 4.4:-60881384 MS.gene34843:CDS
GTGTGATTGCAGAAAGGGTT+TGG 0.412924 4.4:+60881071 None:intergenic
GAATGACACAGTGGACAAAT+TGG 0.413926 4.4:-60881645 MS.gene34843:CDS
AAACAGTGTGATTGCAGAAA+GGG 0.428854 4.4:+60881066 None:intergenic
TGATAGAATAATGAGGCTTA+TGG 0.438357 4.4:-60881144 MS.gene34843:CDS
ATGTTTCCAAGCTTGCAAAC+TGG 0.447747 4.4:-60881568 MS.gene34843:CDS
GGAATTAATTGACTTTATGA+AGG 0.456415 4.4:+60880990 None:intergenic
AGCAGAAAAGCATTCAGAAC+TGG 0.460756 4.4:-60881479 MS.gene34843:CDS
TGGTTTCTGCATGGGCTGGT+TGG 0.463961 4.4:-60881025 MS.gene34843:CDS
TAGTGGATTGGTTTCTGCAT+GGG 0.486842 4.4:-60881033 MS.gene34843:CDS
TGCAGATTTGATTATAGCAT+TGG 0.487810 4.4:-60881105 MS.gene34843:CDS
TGGAACTGTGGATTCAAACT+TGG 0.495305 4.4:-60881321 MS.gene34843:CDS
GGATTGGTTTCTGCATGGGC+TGG 0.505965 4.4:-60881029 MS.gene34843:CDS
TTAGTGGATTGGTTTCTGCA+TGG 0.506709 4.4:-60881034 MS.gene34843:CDS
TTTGTTGAATGAAGGGTTGA+AGG 0.511768 4.4:-60881225 MS.gene34843:CDS
TGAACCTCTTGGATATATCA+GGG 0.540246 4.4:+60881528 None:intergenic
GAAACAGTGTGATTGCAGAA+AGG 0.556504 4.4:+60881065 None:intergenic
CTTAAAGTGAGAGTAGAACC+AGG 0.558345 4.4:+60881419 None:intergenic
CAAGCTTGCAAACTGGCGTG+TGG 0.589710 4.4:-60881561 MS.gene34843:CDS
GAGAAAGCTTAGAAGTTCAG+AGG 0.604684 4.4:-60881198 MS.gene34843:CDS
GAAAAGCATTCAGAACTGGA+AGG 0.628321 4.4:-60881475 MS.gene34843:CDS
AATGCTCTTAGAAGAAACCC+TGG 0.640685 4.4:-60881437 MS.gene34843:CDS
TTAAAGTGAGAGTAGAACCA+GGG 0.653037 4.4:+60881420 None:intergenic
GTTGTCAAGAATTGGAACTG+TGG 0.656125 4.4:-60881333 MS.gene34843:CDS
CTCACCCTGATATATCCAAG+AGG 0.704550 4.4:-60881532 MS.gene34843:CDS
TTCTACCATGAATGACACAG+TGG 0.746124 4.4:-60881654 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAAAGTGATAGAATAATG+AGG - chr4.4:60881500-60881519 MS.gene34843:CDS 20.0%
!!! ATTTCATTTTGTTGAATGAA+GGG - chr4.4:60881419-60881438 MS.gene34843:CDS 20.0%
!! GATTTCATTTTGTTGAATGA+AGG - chr4.4:60881418-60881437 MS.gene34843:CDS 25.0%
ATCCAAGAGGTTCAAAAATT+GGG - chr4.4:60881132-60881151 MS.gene34843:CDS 30.0%
TATCCAAGAGGTTCAAAAAT+TGG - chr4.4:60881131-60881150 MS.gene34843:CDS 30.0%
TGATAGAATAATGAGGCTTA+TGG - chr4.4:60881507-60881526 MS.gene34843:CDS 30.0%
TGCAGATTTGATTATAGCAT+TGG - chr4.4:60881546-60881565 MS.gene34843:CDS 30.0%
TTCTTGACAACCAAAGAAAA+TGG + chr4.4:60881309-60881328 None:intergenic 30.0%
TTTCTTTGGTTGTCAAGAAT+TGG - chr4.4:60881310-60881329 MS.gene34843:CDS 30.0%
! ATGAGTTTCATATCTGCTTT+TGG - chr4.4:60881361-60881380 MS.gene34843:CDS 30.0%
! TCTTCTTTGACCATTTTCTT+TGG - chr4.4:60881296-60881315 MS.gene34843:CDS 30.0%
AAAAATTGGGAAGTAGCTTC+TGG - chr4.4:60881145-60881164 MS.gene34843:CDS 35.0%
AAACAGTGTGATTGCAGAAA+GGG + chr4.4:60881588-60881607 None:intergenic 35.0%
ATCTTCCTAGCAAAAAGAGA+TGG - chr4.4:60881031-60881050 MS.gene34843:CDS 35.0%
GCTTTCTAATTCAGGAGAAA+TGG - chr4.4:60881267-60881286 MS.gene34843:CDS 35.0%
TGAACCTCTTGGATATATCA+GGG + chr4.4:60881126-60881145 None:intergenic 35.0%
TTAAAGTGAGAGTAGAACCA+GGG + chr4.4:60881234-60881253 None:intergenic 35.0%
TTGAACCTCTTGGATATATC+AGG + chr4.4:60881127-60881146 None:intergenic 35.0%
TTGCAAGCTTGGAAACATAT+TGG + chr4.4:60881081-60881100 None:intergenic 35.0%
TTTGTTGAATGAAGGGTTGA+AGG - chr4.4:60881426-60881445 MS.gene34843:CDS 35.0%
! ACTGTTTCTCTTGGTATTAG+TGG - chr4.4:60881601-60881620 MS.gene34843:CDS 35.0%
! TTCCCAATTTTTGAACCTCT+TGG + chr4.4:60881137-60881156 None:intergenic 35.0%
! TTCTCTTGGTATTAGTGGAT+TGG - chr4.4:60881606-60881625 MS.gene34843:CDS 35.0%
!! TTTCATATCTGCTTTTGGTG+AGG - chr4.4:60881366-60881385 MS.gene34843:CDS 35.0%
AATGCTCTTAGAAGAAACCC+TGG - chr4.4:60881214-60881233 MS.gene34843:CDS 40.0%
AGAACTGGAAGGTTTCTTAC+TGG - chr4.4:60881187-60881206 MS.gene34843:CDS 40.0%
ATGTTTCCAAGCTTGCAAAC+TGG - chr4.4:60881083-60881102 MS.gene34843:CDS 40.0%
CTAGCAGTGCTTTCTAATTC+AGG - chr4.4:60881259-60881278 MS.gene34843:CDS 40.0%
CTTAAAGTGAGAGTAGAACC+AGG + chr4.4:60881235-60881254 None:intergenic 40.0%
GAAACAGTGTGATTGCAGAA+AGG + chr4.4:60881589-60881608 None:intergenic 40.0%
GAATGACACAGTGGACAAAT+TGG - chr4.4:60881006-60881025 MS.gene34843:CDS 40.0%
GAGAAAGCTTAGAAGTTCAG+AGG - chr4.4:60881453-60881472 MS.gene34843:CDS 40.0%
GTTGTCAAGAATTGGAACTG+TGG - chr4.4:60881318-60881337 MS.gene34843:CDS 40.0%
TGGAACTGTGGATTCAAACT+TGG - chr4.4:60881330-60881349 MS.gene34843:CDS 40.0%
! CTATGCCATCTCTTTTTGCT+AGG + chr4.4:60881039-60881058 None:intergenic 40.0%
! TGCAATCACACTGTTTCTCT+TGG - chr4.4:60881592-60881611 MS.gene34843:CDS 40.0%
!! AGCAGAAAAGCATTCAGAAC+TGG - chr4.4:60881172-60881191 MS.gene34843:CDS 40.0%
!! CTGGTTCTACTCTCACTTTA+AGG - chr4.4:60881233-60881252 MS.gene34843:CDS 40.0%
!! GAAAAGCATTCAGAACTGGA+AGG - chr4.4:60881176-60881195 MS.gene34843:CDS 40.0%
!! TAGTGGATTGGTTTCTGCAT+GGG - chr4.4:60881618-60881637 MS.gene34843:CDS 40.0%
!! TTAGTGGATTGGTTTCTGCA+TGG - chr4.4:60881617-60881636 MS.gene34843:CDS 40.0%
CTCACCCTGATATATCCAAG+AGG - chr4.4:60881119-60881138 MS.gene34843:CDS 45.0%
GTGTGATTGCAGAAAGGGTT+TGG + chr4.4:60881583-60881602 None:intergenic 45.0%
!! GGGCTGGTTGGTATAGAAAT+TGG - chr4.4:60881638-60881657 MS.gene34843:CDS 45.0%
!! ATCTGCTTTTGGTGAGGCTG+TGG - chr4.4:60881372-60881391 MS.gene34843:CDS 50.0%
!! TCTGCTTTTGGTGAGGCTGT+GGG - chr4.4:60881373-60881392 MS.gene34843:CDS 50.0%
CAAGCTTGCAAACTGGCGTG+TGG - chr4.4:60881090-60881109 MS.gene34843:CDS 55.0%
CACACGCCAGTTTGCAAGCT+TGG + chr4.4:60881092-60881111 None:intergenic 55.0%
!! GGATTGGTTTCTGCATGGGC+TGG - chr4.4:60881622-60881641 MS.gene34843:CDS 55.0%
!! TGGTTTCTGCATGGGCTGGT+TGG - chr4.4:60881626-60881645 MS.gene34843:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 60881004 60881669 60881004 ID=MS.gene34843
chr4.4 mRNA 60881004 60881669 60881004 ID=MS.gene34843.t1;Parent=MS.gene34843
chr4.4 exon 60881004 60881669 60881004 ID=MS.gene34843.t1.exon1;Parent=MS.gene34843.t1
chr4.4 CDS 60881004 60881669 60881004 ID=cds.MS.gene34843.t1;Parent=MS.gene34843.t1
Gene Sequence

>MS.gene34843

ATGAATGACACAGTGGACAAATTGGTGATCTTCCTAGCAAAAAGAGATGGCATAGACAAGCTTGTGAAGACATTCCAATATGTTTCCAAGCTTGCAAACTGGCGTGTGGAAGTCACTCACCCTGATATATCCAAGAGGTTCAAAAATTGGGAAGTAGCTTCTGGTCTCAGCAGAAAAGCATTCAGAACTGGAAGGTTTCTTACTGGATTCAATGCTCTTAGAAGAAACCCTGGTTCTACTCTCACTTTAAGGCTACTAGCAGTGCTTTCTAATTCAGGAGAAATGGTTTATTTCTTCTTTGACCATTTTCTTTGGTTGTCAAGAATTGGAACTGTGGATTCAAACTTGGCAAAGAAGATGAGTTTCATATCTGCTTTTGGTGAGGCTGTGGGATATGTTTTCTTCATTATAGCTGATTTCATTTTGTTGAATGAAGGGTTGAAGGCTGAGAGAAAGCTTAGAAGTTCAGAGGATAATGAGAAAGAGATTCAGAAATTAAAAAGTGATAGAATAATGAGGCTTATGGCTGTTGCTGCTAATGTTGCAGATTTGATTATAGCATTGGCTGAAATTGAGCCAAACCCTTTCTGCAATCACACTGTTTCTCTTGGTATTAGTGGATTGGTTTCTGCATGGGCTGGTTGGTATAGAAATTGGCCTTCATAA

Protein sequence

>MS.gene34843.t1

MNDTVDKLVIFLAKRDGIDKLVKTFQYVSKLANWRVEVTHPDISKRFKNWEVASGLSRKAFRTGRFLTGFNALRRNPGSTLTLRLLAVLSNSGEMVYFFFDHFLWLSRIGTVDSNLAKKMSFISAFGEAVGYVFFIIADFILLNEGLKAERKLRSSEDNEKEIQKLKSDRIMRLMAVAANVADLIIALAEIEPNPFCNHTVSLGISGLVSAWAGWYRNWPS