AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880042859.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 15 sgRNAs with CRISPR-Local

Find 14 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAAGTGAATTAGTGAGTTT+TGG 0.292319 8:+14988019 MsG0880042859.01.T01:CDS
TGCAATGTTTCTTATCCTAA+TGG 0.302754 8:+14988099 MsG0880042859.01.T01:CDS
TGTGGCATGAAGAATCTAAT+TGG 0.340065 8:+14988160 MsG0880042859.01.T01:CDS
AATTCACACTCCTATCCATT+AGG 0.422925 8:-14988114 None:intergenic
AATTCACTTTGATCCCTTGA+GGG 0.473147 8:-14988007 MsG0880042858.01.T01:five_prime_UTR
TGATACTGTTCTTGCCCTCA+AGG 0.513416 8:+14987993 MsG0880042859.01.T01:CDS
CATGAAGAATCTAATTGGAT+AGG 0.522322 8:+14988165 MsG0880042859.01.T01:CDS
TGGTTATGAGAATGATTGGT+TGG 0.532403 8:+14988039 MsG0880042859.01.T01:CDS
AGAAATGGCAACAGCTGCAA+TGG 0.552836 8:+14987946 None:intergenic
TGTTTCTTATCCTAATGGAT+AGG 0.560547 8:+14988104 MsG0880042859.01.T01:CDS
TAATTCACTTTGATCCCTTG+AGG 0.566836 8:-14988008 MsG0880042858.01.T01:five_prime_UTR
TGAGGGCAAGAACAGTATCA+TGG 0.591469 8:-14987990 MsG0880042858.01.T01:exon
ATTAGATTCTTCATGCCACA+TGG 0.600981 8:-14988157 None:intergenic
GATACTGTTCTTGCCCTCAA+GGG 0.624491 8:+14987994 MsG0880042859.01.T01:CDS
TTAGATAATGTGTTGCCATG+TGG 0.734720 8:+14988142 MsG0880042859.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TGCAATGTTTCTTATCCTAA+TGG + Chr8:14988099-14988118 MsG0880042859.01.T01:CDS 30.0%
! CAAAGTGAATTAGTGAGTTT+TGG + Chr8:14988019-14988038 MsG0880042859.01.T01:CDS 30.0%
! TGTTTCTTATCCTAATGGAT+AGG + Chr8:14988104-14988123 MsG0880042859.01.T01:CDS 30.0%
!!! GTTTTGGTTATGAGAATGAT+TGG + Chr8:14988035-14988054 MsG0880042859.01.T01:CDS 30.0%
AATTCACACTCCTATCCATT+AGG - Chr8:14988117-14988136 None:intergenic 35.0%
AATTCACTTTGATCCCTTGA+GGG - Chr8:14988010-14988029 MsG0880042858.01.T01:five_prime_UTR 35.0%
ATTAGATTCTTCATGCCACA+TGG - Chr8:14988160-14988179 None:intergenic 35.0%
TAATTCACTTTGATCCCTTG+AGG - Chr8:14988011-14988030 MsG0880042858.01.T01:five_prime_UTR 35.0%
TGGTTATGAGAATGATTGGT+TGG + Chr8:14988039-14988058 MsG0880042859.01.T01:CDS 35.0%
TGTGGCATGAAGAATCTAAT+TGG + Chr8:14988160-14988179 MsG0880042859.01.T01:CDS 35.0%
TTAGATAATGTGTTGCCATG+TGG + Chr8:14988142-14988161 MsG0880042859.01.T01:CDS 35.0%
GATACTGTTCTTGCCCTCAA+GGG + Chr8:14987994-14988013 MsG0880042859.01.T01:CDS 45.0%
TGAGGGCAAGAACAGTATCA+TGG - Chr8:14987993-14988012 MsG0880042858.01.T01:exon 45.0%
TGATACTGTTCTTGCCCTCA+AGG + Chr8:14987993-14988012 MsG0880042859.01.T01:CDS 45.0%
Chromosome Type Strat End Strand Name
Chr8 gene 14987950 14988186 14987950 ID=MsG0880042859.01;Name=MsG0880042859.01
Chr8 mRNA 14987950 14988186 14987950 ID=MsG0880042859.01.T01;Parent=MsG0880042859.01;Name=MsG0880042859.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|78
Chr8 exon 14987950 14988186 14987950 ID=MsG0880042859.01.T01:exon:1445;Parent=MsG0880042859.01.T01
Chr8 CDS 14987950 14988186 14987950 ID=MsG0880042859.01.T01:cds;Parent=MsG0880042859.01.T01
Gene Sequence

>MsG0880042859.01.T01

ATGGCAACAGCTGCAATGGCAGAAGAAGCACAAGCAGAAGCCATGATACTGTTCTTGCCCTCAAGGGATCAAAGTGAATTAGTGAGTTTTGGTTATGAGAATGATTGGTTGGTGAATATGAAAGAAAATATGATGATTTTGTTAATGTATGCAATGTTTCTTATCCTAATGGATAGGAGTGTGAATTTGATATTAGATAATGTGTTGCCATGTGGCATGAAGAATCTAATTGGATAG

Protein sequence

>MsG0880042859.01.T01

MATAAMAEEAQAEAMILFLPSRDQSELVSFGYENDWLVNMKENMMILLMYAMFLILMDRSVNLILDNVLPCGMKNLIG*