AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0329470


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0329470 MtrunA17_Chr7g0253751 52.500 80 34 1 11 90 24 99 1.95e-23 87.8
Msa0329470 MtrunA17_Chr3g0142071 55.556 63 28 0 27 89 45 107 2.38e-18 79.3
Msa0329470 MtrunA17_Chr2g0311391 54.688 64 29 0 33 96 37 100 9.34e-17 74.3
Msa0329470 MtrunA17_Chr2g0311471 49.206 63 32 0 33 95 37 99 5.83e-14 66.6
Msa0329470 MtrunA17_Chr2g0311091 48.438 64 33 0 33 96 35 98 7.52e-14 64.3
Msa0329470 MtrunA17_Chr2g0311381 48.438 64 33 0 32 95 36 99 1.18e-13 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0329470 AT1G09850.1 45.833 72 38 1 27 97 27 98 8.77e-16 72.4
Msa0329470 AT3G43960.1 41.791 67 39 0 27 93 36 102 7.06e-13 63.9
Msa0329470 AT4G35350.1 51.515 66 31 1 28 93 47 111 9.36e-13 63.5
Msa0329470 AT4G35350.2 51.515 66 31 1 28 93 47 111 1.02e-12 63.2

Find 41 sgRNAs with CRISPR-Local

Find 108 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAATGGAACAACCATAAT+TGG 0.195557 2_4:-43078960 None:intergenic
TTGGTTGTGTTGAGTGATTC+TGG 0.214560 2_4:-43078870 None:intergenic
TGATCAGTTTATGAAATAAA+AGG 0.223384 2_4:-43078476 None:intergenic
TCCATCTTAATATCAATAAA+TGG 0.249277 2_4:+43079215 Msa0329470:three_prime_UTR
GAGGAATTTACGTCCAATTA+TGG 0.252320 2_4:+43078947 Msa0329470:CDS
TAAGATGGAAGTTCAAGATT+TGG 0.319820 2_4:-43079201 None:intergenic
TTTGATTATATTAAAACTAC+TGG 0.347906 2_4:+43079129 Msa0329470:three_prime_UTR
AAACTTCATTGATGTTTGAC+TGG 0.385671 2_4:-43079028 None:intergenic
TTCTTGAAGCTTGGAGTTGG+AGG 0.392872 2_4:-43078612 None:intergenic
TGAGTAATTCAACTCGTAGA+TGG 0.395615 2_4:-43078528 None:intergenic
GTAGCTTCAAAATTTGTCAT+AGG 0.399302 2_4:+43078500 Msa0329470:five_prime_UTR
AGAATAACAAACCGATGGTT+GGG 0.404177 2_4:-43078645 None:intergenic
CTGTTCTTGAAGCTTGGAGT+TGG 0.411197 2_4:-43078615 None:intergenic
CCGGATGATCTTGAAAAGTT+AGG 0.423858 2_4:+43078998 Msa0329470:CDS
TCCATGAAATATTTGAAAAG+TGG 0.434083 2_4:+43078771 Msa0329470:CDS
AGGAATCTGTTCTTGAAGCT+TGG 0.443942 2_4:-43078621 None:intergenic
ACCATTTATTGATATTAAGA+TGG 0.454456 2_4:-43079216 None:intergenic
CAACTATTAACACTTCATCT+TGG 0.461354 2_4:-43079176 None:intergenic
AACGTTGTGTCTCTATTCAA+TGG 0.463697 2_4:+43078571 Msa0329470:exon
GATCAGTTTATGAAATAAAA+GGG 0.463851 2_4:-43078475 None:intergenic
AATTGTGAATATAAGGCTCC+AGG 0.482310 2_4:+43078740 Msa0329470:intron
TGAATATAAGGCTCCAGGTT+CGG 0.485465 2_4:+43078745 Msa0329470:intron
CGAAGGTGAGATCAGAGAAC+TGG 0.488939 2_4:-43078925 None:intergenic
TGATTTCAACGATGTCCATC+CGG 0.494998 2_4:+43078979 Msa0329470:CDS
TAGAATAACAAACCGATGGT+TGG 0.501861 2_4:-43078646 None:intergenic
GTTCTCTGATCTCACCTTCG+AGG 0.507841 2_4:+43078928 Msa0329470:CDS
ATGGACATCGTTGAAATCAA+TGG 0.510440 2_4:-43078975 None:intergenic
CGACAAAACATATTCTTCAA+AGG 0.513620 2_4:+43078805 Msa0329470:CDS
ATTTCATGGATGTCCGAACC+TGG 0.518460 2_4:-43078758 None:intergenic
ATATAATCAAAGGTGGTGAC+AGG 0.523055 2_4:-43079117 None:intergenic
AACTTCATTGATGTTTGACT+GGG 0.536300 2_4:-43079027 None:intergenic
ATTAAAACTACTGGGCTAAG+AGG 0.546739 2_4:+43079138 Msa0329470:three_prime_UTR
GAATAACAAACCGATGGTTG+GGG 0.564507 2_4:-43078644 None:intergenic
TTGATTATATTAAAACTACT+GGG 0.577047 2_4:+43079130 Msa0329470:three_prime_UTR
ACAGATTCCTCCCCAACCAT+CGG 0.584110 2_4:+43078634 Msa0329470:CDS
TGAGATCAGAGAACTGGTTG+AGG 0.597092 2_4:-43078919 None:intergenic
AATATTTGAAAAGTGGTGCA+AGG 0.602264 2_4:+43078778 Msa0329470:CDS
AAAACTACTGGGCTAAGAGG+AGG 0.615728 2_4:+43079141 Msa0329470:three_prime_UTR
TAACAAACCGATGGTTGGGG+AGG 0.622332 2_4:-43078641 None:intergenic
ATTGGACGTAAATTCCTCGA+AGG 0.638330 2_4:-43078942 None:intergenic
ATTGAATAGAGACACAACGT+TGG 0.690423 2_4:-43078569 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATTTTAATTGTGAATATA+AGG + chr2_4:43078733-43078752 Msa0329470:intron 10.0%
!!! TAATTTTAATTGTGAATATA+AGG + chr2_4:43078733-43078752 Msa0329470:intron 10.0%
!!! AGTAGTTTTAATATAATCAA+AGG - chr2_4:43079130-43079149 None:intergenic 15.0%
!! TTTGATTATATTAAAACTAC+TGG + chr2_4:43079129-43079148 Msa0329470:three_prime_UTR 15.0%
!! TTGATTATATTAAAACTACT+GGG + chr2_4:43079130-43079149 Msa0329470:three_prime_UTR 15.0%
!!! AGTAGTTTTAATATAATCAA+AGG - chr2_4:43079130-43079149 None:intergenic 15.0%
!! TTTGATTATATTAAAACTAC+TGG + chr2_4:43079129-43079148 Msa0329470:three_prime_UTR 15.0%
!! TTGATTATATTAAAACTACT+GGG + chr2_4:43079130-43079149 Msa0329470:three_prime_UTR 15.0%
!!! AGTTTTAATATAATCAAAGG+TGG - chr2_4:43079127-43079146 None:intergenic 20.0%
!! TCCATCTTAATATCAATAAA+TGG + chr2_4:43079215-43079234 Msa0329470:three_prime_UTR 20.0%
!! ACCATTTATTGATATTAAGA+TGG - chr2_4:43079219-43079238 None:intergenic 20.0%
!!! AGTTTTAATATAATCAAAGG+TGG - chr2_4:43079127-43079146 None:intergenic 20.0%
!! TCCATCTTAATATCAATAAA+TGG + chr2_4:43079215-43079234 Msa0329470:three_prime_UTR 20.0%
!! ACCATTTATTGATATTAAGA+TGG - chr2_4:43079219-43079238 None:intergenic 20.0%
! AAATCATGAGAGAAATTGAA+GGG - chr2_4:43078673-43078692 None:intergenic 25.0%
! AAAATCATGAGAGAAATTGA+AGG - chr2_4:43078674-43078693 None:intergenic 25.0%
! TCCATGAAATATTTGAAAAG+TGG + chr2_4:43078771-43078790 Msa0329470:CDS 25.0%
!!! ACCACTTTTCAAATATTTCA+TGG - chr2_4:43078775-43078794 None:intergenic 25.0%
! ACAAAAAACACCACAATTAT+TGG - chr2_4:43079057-43079076 None:intergenic 25.0%
! AAATCATGAGAGAAATTGAA+GGG - chr2_4:43078673-43078692 None:intergenic 25.0%
! AAAATCATGAGAGAAATTGA+AGG - chr2_4:43078674-43078693 None:intergenic 25.0%
! TCCATGAAATATTTGAAAAG+TGG + chr2_4:43078771-43078790 Msa0329470:CDS 25.0%
!!! ACCACTTTTCAAATATTTCA+TGG - chr2_4:43078775-43078794 None:intergenic 25.0%
! ACAAAAAACACCACAATTAT+TGG - chr2_4:43079057-43079076 None:intergenic 25.0%
GTAGCTTCAAAATTTGTCAT+AGG + chr2_4:43078500-43078519 Msa0329470:five_prime_UTR 30.0%
AATATTTGAAAAGTGGTGCA+AGG + chr2_4:43078778-43078797 Msa0329470:CDS 30.0%
CGACAAAACATATTCTTCAA+AGG + chr2_4:43078805-43078824 Msa0329470:CDS 30.0%
!! TGTAACCAGAATTTTTCAGT+TGG - chr2_4:43078892-43078911 None:intergenic 30.0%
! AAAAATTCTGGTTACACTCT+AGG + chr2_4:43078896-43078915 Msa0329470:CDS 30.0%
ATCAATGGAACAACCATAAT+TGG - chr2_4:43078963-43078982 None:intergenic 30.0%
AACTTCATTGATGTTTGACT+GGG - chr2_4:43079030-43079049 None:intergenic 30.0%
AAACTTCATTGATGTTTGAC+TGG - chr2_4:43079031-43079050 None:intergenic 30.0%
!! GAAGTTTTGTCCAATAATTG+TGG + chr2_4:43079044-43079063 Msa0329470:exon 30.0%
CAACTATTAACACTTCATCT+TGG - chr2_4:43079179-43079198 None:intergenic 30.0%
TAAGATGGAAGTTCAAGATT+TGG - chr2_4:43079204-43079223 None:intergenic 30.0%
GTAGCTTCAAAATTTGTCAT+AGG + chr2_4:43078500-43078519 Msa0329470:five_prime_UTR 30.0%
AATATTTGAAAAGTGGTGCA+AGG + chr2_4:43078778-43078797 Msa0329470:CDS 30.0%
CGACAAAACATATTCTTCAA+AGG + chr2_4:43078805-43078824 Msa0329470:CDS 30.0%
!! TGTAACCAGAATTTTTCAGT+TGG - chr2_4:43078892-43078911 None:intergenic 30.0%
! AAAAATTCTGGTTACACTCT+AGG + chr2_4:43078896-43078915 Msa0329470:CDS 30.0%
ATCAATGGAACAACCATAAT+TGG - chr2_4:43078963-43078982 None:intergenic 30.0%
AACTTCATTGATGTTTGACT+GGG - chr2_4:43079030-43079049 None:intergenic 30.0%
AAACTTCATTGATGTTTGAC+TGG - chr2_4:43079031-43079050 None:intergenic 30.0%
!! GAAGTTTTGTCCAATAATTG+TGG + chr2_4:43079044-43079063 Msa0329470:exon 30.0%
CAACTATTAACACTTCATCT+TGG - chr2_4:43079179-43079198 None:intergenic 30.0%
TAAGATGGAAGTTCAAGATT+TGG - chr2_4:43079204-43079223 None:intergenic 30.0%
TGAGTAATTCAACTCGTAGA+TGG - chr2_4:43078531-43078550 None:intergenic 35.0%
ATTGAATAGAGACACAACGT+TGG - chr2_4:43078572-43078591 None:intergenic 35.0%
AACGTTGTGTCTCTATTCAA+TGG + chr2_4:43078571-43078590 Msa0329470:exon 35.0%
AGAATAACAAACCGATGGTT+GGG - chr2_4:43078648-43078667 None:intergenic 35.0%
TAGAATAACAAACCGATGGT+TGG - chr2_4:43078649-43078668 None:intergenic 35.0%
AATTGTGAATATAAGGCTCC+AGG + chr2_4:43078740-43078759 Msa0329470:intron 35.0%
CAAAGGAAGAAAAACAGTAC+AGG + chr2_4:43078822-43078841 Msa0329470:CDS 35.0%
CACAACCAACTGAAAAATTC+TGG + chr2_4:43078884-43078903 Msa0329470:CDS 35.0%
GAGGAATTTACGTCCAATTA+TGG + chr2_4:43078947-43078966 Msa0329470:CDS 35.0%
ATGGACATCGTTGAAATCAA+TGG - chr2_4:43078978-43078997 None:intergenic 35.0%
!! CCTAACTTTTCAAGATCATC+CGG - chr2_4:43079001-43079020 None:intergenic 35.0%
ATATAATCAAAGGTGGTGAC+AGG - chr2_4:43079120-43079139 None:intergenic 35.0%
ATTAAAACTACTGGGCTAAG+AGG + chr2_4:43079138-43079157 Msa0329470:three_prime_UTR 35.0%
TGAGTAATTCAACTCGTAGA+TGG - chr2_4:43078531-43078550 None:intergenic 35.0%
ATTGAATAGAGACACAACGT+TGG - chr2_4:43078572-43078591 None:intergenic 35.0%
AACGTTGTGTCTCTATTCAA+TGG + chr2_4:43078571-43078590 Msa0329470:exon 35.0%
AGAATAACAAACCGATGGTT+GGG - chr2_4:43078648-43078667 None:intergenic 35.0%
TAGAATAACAAACCGATGGT+TGG - chr2_4:43078649-43078668 None:intergenic 35.0%
AATTGTGAATATAAGGCTCC+AGG + chr2_4:43078740-43078759 Msa0329470:intron 35.0%
CAAAGGAAGAAAAACAGTAC+AGG + chr2_4:43078822-43078841 Msa0329470:CDS 35.0%
CACAACCAACTGAAAAATTC+TGG + chr2_4:43078884-43078903 Msa0329470:CDS 35.0%
GAGGAATTTACGTCCAATTA+TGG + chr2_4:43078947-43078966 Msa0329470:CDS 35.0%
ATGGACATCGTTGAAATCAA+TGG - chr2_4:43078978-43078997 None:intergenic 35.0%
!! CCTAACTTTTCAAGATCATC+CGG - chr2_4:43079001-43079020 None:intergenic 35.0%
ATATAATCAAAGGTGGTGAC+AGG - chr2_4:43079120-43079139 None:intergenic 35.0%
ATTAAAACTACTGGGCTAAG+AGG + chr2_4:43079138-43079157 Msa0329470:three_prime_UTR 35.0%
AGGAATCTGTTCTTGAAGCT+TGG - chr2_4:43078624-43078643 None:intergenic 40.0%
GAATAACAAACCGATGGTTG+GGG - chr2_4:43078647-43078666 None:intergenic 40.0%
GGGATAGAATAACAAACCGA+TGG - chr2_4:43078653-43078672 None:intergenic 40.0%
TGAATATAAGGCTCCAGGTT+CGG + chr2_4:43078745-43078764 Msa0329470:intron 40.0%
TTGGTTGTGTTGAGTGATTC+TGG - chr2_4:43078873-43078892 None:intergenic 40.0%
ATTGGACGTAAATTCCTCGA+AGG - chr2_4:43078945-43078964 None:intergenic 40.0%
TGATTTCAACGATGTCCATC+CGG + chr2_4:43078979-43078998 Msa0329470:CDS 40.0%
!! ACTTTTCAAGATCATCCGGA+TGG - chr2_4:43078997-43079016 None:intergenic 40.0%
CCGGATGATCTTGAAAAGTT+AGG + chr2_4:43078998-43079017 Msa0329470:CDS 40.0%
AGGAATCTGTTCTTGAAGCT+TGG - chr2_4:43078624-43078643 None:intergenic 40.0%
GAATAACAAACCGATGGTTG+GGG - chr2_4:43078647-43078666 None:intergenic 40.0%
GGGATAGAATAACAAACCGA+TGG - chr2_4:43078653-43078672 None:intergenic 40.0%
TGAATATAAGGCTCCAGGTT+CGG + chr2_4:43078745-43078764 Msa0329470:intron 40.0%
TTGGTTGTGTTGAGTGATTC+TGG - chr2_4:43078873-43078892 None:intergenic 40.0%
ATTGGACGTAAATTCCTCGA+AGG - chr2_4:43078945-43078964 None:intergenic 40.0%
TGATTTCAACGATGTCCATC+CGG + chr2_4:43078979-43078998 Msa0329470:CDS 40.0%
!! ACTTTTCAAGATCATCCGGA+TGG - chr2_4:43078997-43079016 None:intergenic 40.0%
CCGGATGATCTTGAAAAGTT+AGG + chr2_4:43078998-43079017 Msa0329470:CDS 40.0%
TTCTTGAAGCTTGGAGTTGG+AGG - chr2_4:43078615-43078634 None:intergenic 45.0%
CTGTTCTTGAAGCTTGGAGT+TGG - chr2_4:43078618-43078637 None:intergenic 45.0%
ATTTCATGGATGTCCGAACC+TGG - chr2_4:43078761-43078780 None:intergenic 45.0%
TGAGATCAGAGAACTGGTTG+AGG - chr2_4:43078922-43078941 None:intergenic 45.0%
AAAACTACTGGGCTAAGAGG+AGG + chr2_4:43079141-43079160 Msa0329470:three_prime_UTR 45.0%
TTCTTGAAGCTTGGAGTTGG+AGG - chr2_4:43078615-43078634 None:intergenic 45.0%
CTGTTCTTGAAGCTTGGAGT+TGG - chr2_4:43078618-43078637 None:intergenic 45.0%
ATTTCATGGATGTCCGAACC+TGG - chr2_4:43078761-43078780 None:intergenic 45.0%
TGAGATCAGAGAACTGGTTG+AGG - chr2_4:43078922-43078941 None:intergenic 45.0%
AAAACTACTGGGCTAAGAGG+AGG + chr2_4:43079141-43079160 Msa0329470:three_prime_UTR 45.0%
ACAGATTCCTCCCCAACCAT+CGG + chr2_4:43078634-43078653 Msa0329470:CDS 50.0%
TAACAAACCGATGGTTGGGG+AGG - chr2_4:43078644-43078663 None:intergenic 50.0%
CGAAGGTGAGATCAGAGAAC+TGG - chr2_4:43078928-43078947 None:intergenic 50.0%
GTTCTCTGATCTCACCTTCG+AGG + chr2_4:43078928-43078947 Msa0329470:CDS 50.0%
ACAGATTCCTCCCCAACCAT+CGG + chr2_4:43078634-43078653 Msa0329470:CDS 50.0%
TAACAAACCGATGGTTGGGG+AGG - chr2_4:43078644-43078663 None:intergenic 50.0%
CGAAGGTGAGATCAGAGAAC+TGG - chr2_4:43078928-43078947 None:intergenic 50.0%
GTTCTCTGATCTCACCTTCG+AGG + chr2_4:43078928-43078947 Msa0329470:CDS 50.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 43078477 43079300 43078477 ID=Msa0329470;Name=Msa0329470
chr2_4 mRNA 43078477 43079300 43078477 ID=Msa0329470-mRNA-1;Parent=Msa0329470;Name=Msa0329470-mRNA-1;_AED=0.00;_eAED=0.00;_QI=113|1|1|1|1|1|2|255|118
chr2_4 exon 43078477 43078655 43078477 ID=Msa0329470-mRNA-1:exon:13178;Parent=Msa0329470-mRNA-1
chr2_4 exon 43078755 43079300 43078755 ID=Msa0329470-mRNA-1:exon:13179;Parent=Msa0329470-mRNA-1
chr2_4 five_prime_UTR 43078477 43078589 43078477 ID=Msa0329470-mRNA-1:five_prime_utr;Parent=Msa0329470-mRNA-1
chr2_4 CDS 43078590 43078655 43078590 ID=Msa0329470-mRNA-1:cds;Parent=Msa0329470-mRNA-1
chr2_4 CDS 43078755 43079045 43078755 ID=Msa0329470-mRNA-1:cds;Parent=Msa0329470-mRNA-1
chr2_4 three_prime_UTR 43079046 43079300 43079046 ID=Msa0329470-mRNA-1:three_prime_utr;Parent=Msa0329470-mRNA-1
Gene Sequence

>Msa0329470

ATGGAAGTAAACAAAGAGCTAACCTCCAACTCCAAGCTTCAAGAACAGATTCCTCCCCAACCATCGGCTCCAGGTTCGGACATCCATGAAATATTTGAAAAGTGGTGCAAGGAATTCGACAAAACATATTCTTCAAAGGAAGAAAAACAGTACAGGTTCAAAGTGTTTGAAGAAAGATATGCCAGAATCACTCAACACAACCAACTGAAAAATTCTGGTTACACTCTAGGCCTCAACCAGTTCTCTGATCTCACCTTCGAGGAATTTACGTCCAATTATGGTTGTTCCATTGATTTCAACGATGTCCATCCGGATGATCTTGAAAAGTTAGGCATTAACCCAGTCAAACATCAATGA

Protein sequence

>Msa0329470

MEVNKELTSNSKLQEQIPPQPSAPGSDIHEIFEKWCKEFDKTYSSKEEKQYRFKVFEERYARITQHNQLKNSGYTLGLNQFSDLTFEEFTSNYGCSIDFNDVHPDDLEKLGINPVKHQ*