AlfalfaGEDB | Msa1451200

M. sativa cultivar zhongmu-4 / Msa1451200

KEGG: K16189
GO: GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006725 GO:0006778 GO:0006779 GO:0006783 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009058 GO:0009314 GO:0009416 GO:0009605 GO:0009606 GO:0009628 GO:0009629 GO:0009630 GO:0009639 GO:0009719 GO:0009725 GO:0009739 GO:0009740 GO:0009755 GO:0009889 GO:0009959 GO:0009987 GO:0010017 GO:0010029 GO:0010033 GO:0010099 GO:0010100 GO:0010114 GO:0010161 GO:0010187 GO:0010313 GO:0010468 GO:0010476 GO:0010556 GO:0015994 GO:0015995 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0023052 GO:0031323 GO:0031326 GO:0032870 GO:0033013 GO:0033014 GO:0033993 GO:0034641 GO:0042168 GO:0042221 GO:0042440 GO:0042802 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044249 GO:0044271 GO:0044424 GO:0044464 GO:0046148 GO:0046483 GO:0048519 GO:0048580 GO:0048581 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051186 GO:0051188 GO:0051239 GO:0051241 GO:0051252 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0071214 GO:0071229 GO:0071310 GO:0071370 GO:0071396 GO:0071478 GO:0071482 GO:0071489 GO:0071491 GO:0071495 GO:0071704 GO:0080090 GO:0097159 GO:0104004 GO:0140110 GO:1900140 GO:1901360 GO:1901362 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1901700 GO:1901701 GO:1903506 GO:2000026 GO:2000030 GO:2000112 GO:2001141
PFAM: PF00010.29

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1451200	sp\|Q8GZM7\|PIF1_ARATH	41.650	497	171	28	21	466	26	454	1.86e-65	223

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1451200	G7KZK8	89.485	485	28	6	2	485	3	465	0.0	821

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TFs/TRs:

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Gene ID	Type	Classification
Msa1451200	TF	bHLH

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000610	Msa1451200	0.888299	6.679145e-73	-8.615850e-47
Msa0000800	Msa1451200	-0.864355	1.290663e-64	-8.615850e-47
Msa0001040	Msa1451200	0.834414	3.038821e-56	-8.615850e-47
Msa0001510	Msa1451200	0.895270	1.124197e-75	-8.615850e-47
Msa0001550	Msa1451200	0.827134	1.849593e-54	-8.615850e-47
Msa0002110	Msa1451200	0.925553	1.568761e-90	-8.615850e-47
Msa0002690	Msa1451200	0.920989	6.351448e-88	-8.615850e-47
Msa0002700	Msa1451200	0.914193	2.553639e-84	-8.615850e-47
Msa0003340	Msa1451200	0.904585	1.052658e-79	-8.615850e-47
Msa0004180	Msa1451200	0.931505	3.420761e-94	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1451200	MtrunA17_Chr7g0262691	89.485	485	28	6	2	485	3	465	0.0	821
Msa1451200	MtrunA17_Chr1g0182791	54.291	536	170	24	1	475	1	522	1.91e-145	429
Msa1451200	MtrunA17_Chr3g0095391	32.525	495	225	19	22	458	21	464	2.00e-51	182
Msa1451200	MtrunA17_Chr1g0193321	55.195	154	52	5	210	358	403	544	1.16e-35	142
Msa1451200	MtrunA17_Chr7g0271251	42.453	212	95	6	194	396	357	550	3.22e-30	125
Msa1451200	MtrunA17_Chr1g0153241	68.293	82	22	1	272	353	85	162	6.04e-27	110
Msa1451200	MtrunA17_Chr5g0402031	70.423	71	17	1	281	351	114	180	1.13e-22	99.4
Msa1451200	MtrunA17_Chr7g0270871	48.092	131	46	6	264	381	186	307	3.13e-22	98.6
Msa1451200	MtrunA17_Chr7g0231461	58.228	79	33	0	256	334	281	359	1.51e-21	97.8
Msa1451200	MtrunA17_Chr4g0074161	45.161	93	48	1	275	364	209	301	3.77e-17	84.0

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1451200	AT2G20180.4	41.650	497	171	28	21	466	26	454	1.89e-66	223
Msa1451200	AT2G20180.6	41.650	497	171	28	21	466	26	454	1.89e-66	223
Msa1451200	AT2G20180.2	41.650	497	171	28	21	466	26	454	1.89e-66	223
Msa1451200	AT2G20180.3	41.650	497	171	28	21	466	26	454	1.89e-66	223
Msa1451200	AT2G20180.7	42.254	426	153	24	82	466	10	383	4.34e-59	202
Msa1451200	AT2G20180.1	42.254	426	153	24	82	466	10	383	4.34e-59	202
Msa1451200	AT2G20180.5	42.254	426	153	24	82	466	10	383	4.34e-59	202
Msa1451200	AT4G28800.1	34.437	453	216	18	25	453	16	411	7.42e-52	184
Msa1451200	AT2G20180.8	42.165	351	126	20	21	332	26	338	1.09e-50	177
Msa1451200	AT4G28800.2	33.408	449	214	17	28	453	31	417	1.56e-48	175

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Find 173 sgRNAs with CRISPR-Local

Find 395 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTTGGCATACTGTATT+AGG	0.288340	tig0033260:+12395	None:intergenic
AAACAGAATAGTGTAAATGA+GGG	0.569975	tig0033260:+12274	None:intergenic
AAACATCTTCTTATCTATCT+GGG	0.509776	tig0033260:-17729	Msa1451200:five_prime_UTR
AAACCAGAAACCCGCCAGTC+AGG	0.490474	tig0033260:-12885	Msa1451200:intron
AAAGAGATCTCGTGCTGCTG+AGG	0.571066	tig0033260:-14880	Msa1451200:CDS
AAAGAGGAAGGGAGTAGAAG+CGG	0.541766	tig0033260:-16646	Msa1451200:CDS
AAAGCAAAGCAAATTAAAGT+CGG	0.621251	tig0033260:+17529	None:intergenic
AAAGGAGGCATCACGAACCT+TGG	0.572272	tig0033260:+13722	None:intergenic
AACGGAAGGAGACACCGAAG+TGG	0.694309	tig0033260:-16757	Msa1451200:CDS
AACTCCGACGATTCAACTCG+CGG	0.628283	tig0033260:-17194	Msa1451200:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GAAACATCTTCTTATCTATC+TGG	-	tig0033260:12120-12139	Msa1451200:three_prime_UTR	30.0%
AGGTGTAATTAAAAGATGAC+AGG	+	tig0033260:12298-12317	None:intergenic	30.0%
TCATGACATGACATATTAGT+GGG	+	tig0033260:12347-12366	None:intergenic	30.0%
TTCATGACATGACATATTAG+TGG	+	tig0033260:12348-12367	None:intergenic	30.0%
TAATAAACGGTAAGATGAAG+CGG	+	tig0033260:12490-12509	None:intergenic	30.0%
CTTCATGCAAGAAAATGAAA+TGG	-	tig0033260:12736-12755	Msa1451200:intron	30.0%
AAGAAAATGAAATGGCTTCA+TGG	-	tig0033260:12744-12763	Msa1451200:CDS	30.0%
GAAAAATGCATTCTTGAACA+TGG	-	tig0033260:14144-14163	Msa1451200:intron	30.0%
TATCAAATTGTGCTTAGTTG+TGG	-	tig0033260:14194-14213	Msa1451200:intron	30.0%
TACGATAATCATTTAAGCTG+AGG	-	tig0033260:14360-14379	Msa1451200:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0033260	gene	12120	17752	12120	ID=Msa1451200;Name=Msa1451200
tig0033260	mRNA	12120	17752	12120	ID=Msa1451200-mRNA-1;Parent=Msa1451200;Name=Msa1451200-mRNA-1;_AED=0.25;_eAED=0.25;_QI=281\|0.77\|0.8\|1\|1\|1\|10\|271\|549
tig0033260	exon	12120	12435	12120	ID=Msa1451200-mRNA-1:exon:17890;Parent=Msa1451200-mRNA-1
tig0033260	exon	12516	12597	12516	ID=Msa1451200-mRNA-1:exon:17889;Parent=Msa1451200-mRNA-1
tig0033260	exon	12742	12811	12742	ID=Msa1451200-mRNA-1:exon:17888;Parent=Msa1451200-mRNA-1
tig0033260	exon	12886	12949	12886	ID=Msa1451200-mRNA-1:exon:17887;Parent=Msa1451200-mRNA-1
tig0033260	exon	13544	13936	13544	ID=Msa1451200-mRNA-1:exon:17886;Parent=Msa1451200-mRNA-1
tig0033260	exon	14519	14584	14519	ID=Msa1451200-mRNA-1:exon:17885;Parent=Msa1451200-mRNA-1
tig0033260	exon	14702	14767	14702	ID=Msa1451200-mRNA-1:exon:17884;Parent=Msa1451200-mRNA-1
tig0033260	exon	14860	14955	14860	ID=Msa1451200-mRNA-1:exon:17883;Parent=Msa1451200-mRNA-1
tig0033260	exon	16623	17313	16623	ID=Msa1451200-mRNA-1:exon:17882;Parent=Msa1451200-mRNA-1
tig0033260	exon	17395	17752	17395	ID=Msa1451200-mRNA-1:exon:17881;Parent=Msa1451200-mRNA-1
tig0033260	five_prime_UTR	17472	17752	17472	ID=Msa1451200-mRNA-1:five_prime_utr;Parent=Msa1451200-mRNA-1
tig0033260	CDS	17395	17471	17395	ID=Msa1451200-mRNA-1:cds;Parent=Msa1451200-mRNA-1
tig0033260	CDS	16623	17313	16623	ID=Msa1451200-mRNA-1:cds;Parent=Msa1451200-mRNA-1
tig0033260	CDS	14860	14955	14860	ID=Msa1451200-mRNA-1:cds;Parent=Msa1451200-mRNA-1
tig0033260	CDS	14702	14767	14702	ID=Msa1451200-mRNA-1:cds;Parent=Msa1451200-mRNA-1
tig0033260	CDS	14519	14584	14519	ID=Msa1451200-mRNA-1:cds;Parent=Msa1451200-mRNA-1
tig0033260	CDS	13544	13936	13544	ID=Msa1451200-mRNA-1:cds;Parent=Msa1451200-mRNA-1
tig0033260	CDS	12886	12949	12886	ID=Msa1451200-mRNA-1:cds;Parent=Msa1451200-mRNA-1
tig0033260	CDS	12742	12811	12742	ID=Msa1451200-mRNA-1:cds;Parent=Msa1451200-mRNA-1
tig0033260	CDS	12516	12597	12516	ID=Msa1451200-mRNA-1:cds;Parent=Msa1451200-mRNA-1
tig0033260	CDS	12391	12435	12391	ID=Msa1451200-mRNA-1:cds;Parent=Msa1451200-mRNA-1
tig0033260	three_prime_UTR	12120	12390	12120	ID=Msa1451200-mRNA-1:three_prime_utr;Parent=Msa1451200-mRNA-1

Gene Sequence

>Msa1451200

ATGAACTACTGCGTTTCATATTTCGAATCTCAAATGGATCAACAAGCAGGATTACCTCGTCCTAAGAAACCAATCATTGCAGATGATGAGATCATGGAGCTGTTATGGAAAAACGGCCAAGTCGTTACACAGAGGCCAAACCACCGTCATCTGAAGAAACCACTGCCGGTGAAAAACTCCGACGATTCAACTCGCGGCGGAGGAGCTTCATCGGCGAGGGAGATTCGACCAGTAGAAAACTACAATCAATATCTCTTCATGCAAGAAAATGAAATGGCTTCATGGCTTCATTACGATGATTCTCCTTTCGAACAAACCTTTTCCACCGATTTCCTTAACCCGCCGCCGACGGTTAATAACAACAGTACAATACAAACTCCGACGTCGGTGGTTCCTTCTCGTCCTCCAATTCCCCCGCCACGGAGGATAGGGCACGTGCAGCCTGAGACACCGAACTTCGCGTACTTTGCGAAGCACGGTGTAAGAACCGAGCCAGGACCGTCGTCAATTTTGAACACTGCTGCGAGGGAATCAACAGTGGTGGATTCGTGTGATACACCAGCTGTGATGCCGGCGGCGTTTTCGGAGACGGTAAGAAGTTTAGCGGATCAAACGGAAGGAGACACCGAAGTGGCGGTGGTTTCGGCAACTTTTGATGAACCGGGCGGTTCAAGTAGCAGTGAAGAAGCTGAACCGGTTGGGAAGGTAGCGGAGCAGGACCGAAAGAGGAAGGGAGTAGAAGCGGAGGAATGGGAATATCAGAGCGAGGATGTTGATTTTGAATCTGCGGAAGCCCAAAAAAATATCAGTGGATCATCTACAAAGAGATCTCGTGCTGCTGAGGTCCATAATCTCTCAGAAAGGAGGCGTCGTGATCGGATTAATGAAAAGATGAAAGCTCTGCAAGAACTTATACCTCGATCTAATAAGTCTGACAAAGCATCTATGCTGGATGAAGCAATTGATTACTTGAAGTCGCTGCAGTTACAAGTGCAGAGAGTACAACTTATGCAGATGATGTCCATGGGATGTGGCATGGTACCTATGATGTTTCCTGGAATCCAGCAGTATATGCCAACAGTTGGAATGGGAATGGGGATGGGTATGGAAATGGGAATAAACAGACCAGTAATGCCATTTCCCAATATGTTATCCGGTTCTGCTTTGCCTGCAGCCGCGAATTTGGGACCAAGGTTCGTGATGCCTCCTTTCCACATGCCGCATGTTCCTACACCTGGTTCATCCAGAATGCAAGCAGCAAATCAGGTAGATAATAATGTGATCACCTCAGCTGGAACACATGATTCAAATCAGTCACGTATCCCAAATTTCAGTGATCCTTATCAACAATATCTTGGACCCCACCAGATGCAGTTTCAATTGATGCAGAATCAAGCAATGAACCAACCAAATGTCAGCAGGCCTCTTGAGAAACCAGAAACCCGCCAGTCAGCTCTTGTCCTGTGTTATGGGTTTCTGGAGCACAGTGACTTCCAGATCTGGTTGTTTACCATGTCAATGAAGGCTGGCTATGCAGATGGTGCTTCTTATTATTGTATATTCTCTTATACTACTAGCTCTCAATATGGTACTACTAGTATTATTGTTATATTTTCTGCCACTCCTAATACAGTATGCCAAGTTTTGTAG

Protein sequence

>Msa1451200

MNYCVSYFESQMDQQAGLPRPKKPIIADDEIMELLWKNGQVVTQRPNHRHLKKPLPVKNSDDSTRGGGASSAREIRPVENYNQYLFMQENEMASWLHYDDSPFEQTFSTDFLNPPPTVNNNSTIQTPTSVVPSRPPIPPPRRIGHVQPETPNFAYFAKHGVRTEPGPSSILNTAARESTVVDSCDTPAVMPAAFSETVRSLADQTEGDTEVAVVSATFDEPGGSSSSEEAEPVGKVAEQDRKRKGVEAEEWEYQSEDVDFESAEAQKNISGSSTKRSRAAEVHNLSERRRRDRINEKMKALQELIPRSNKSDKASMLDEAIDYLKSLQLQVQRVQLMQMMSMGCGMVPMMFPGIQQYMPTVGMGMGMGMEMGINRPVMPFPNMLSGSALPAAANLGPRFVMPPFHMPHVPTPGSSRMQAANQVDNNVITSAGTHDSNQSRIPNFSDPYQQYLGPHQMQFQLMQNQAMNQPNVSRPLEKPETRQSALVLCYGFLEHSDFQIWLFTMSMKAGYADGASYYCIFSYTTSSQYGTTSIIVIFSATPNTVCQVL*