AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0343240


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0343240 MtrunA17_Chr3g0123101 94.595 222 11 1 1 222 1 221 7.33e-137 382
Msa0343240 MtrunA17_Chr3g0099321 44.206 233 102 9 4 222 17 235 7.18e-56 177
Msa0343240 MtrunA17_Chr3g0127731 41.250 160 74 4 64 222 95 235 1.20e-33 120
Msa0343240 MtrunA17_Chr4g0020981 33.121 157 86 5 68 222 70 209 7.65e-21 86.7
Msa0343240 MtrunA17_Chr7g0265191 43.590 78 38 2 132 208 10 82 2.53e-13 63.9
Msa0343240 MtrunA17_Chr4g0046741 34.783 92 57 2 124 212 38 129 4.92e-13 64.3
Msa0343240 MtrunA17_Chr5g0436181 27.941 136 81 4 81 207 7 134 2.04e-12 62.8
Msa0343240 MtrunA17_Chr4g0003341 35.088 114 64 2 119 222 118 231 2.58e-12 63.9
Msa0343240 MtrunA17_Chr6g0458261 36.458 96 55 4 119 208 28 123 9.30e-12 60.8
Msa0343240 MtrunA17_Chr6g0475941 28.037 107 72 2 118 221 53 157 4.86e-11 59.3
Msa0343240 MtrunA17_Chr4g0046751 31.915 94 59 2 118 208 55 146 8.11e-11 58.5
Msa0343240 MtrunA17_Chr4g0046821 31.915 94 59 2 118 208 55 146 8.28e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0343240 AT4G27670.1 43.182 176 71 7 56 222 72 227 5.35e-38 132
Msa0343240 AT1G52560.2 40.952 105 58 3 122 222 121 225 7.45e-20 84.7
Msa0343240 AT1G52560.1 40.952 105 58 3 122 222 128 232 8.49e-20 84.7
Msa0343240 AT4G25200.1 33.962 159 88 5 51 208 53 195 5.33e-19 82.0
Msa0343240 AT5G51440.1 32.203 177 99 5 33 208 39 195 2.31e-18 80.5
Msa0343240 AT1G07400.1 31.783 129 72 6 91 209 19 141 1.49e-12 63.5
Msa0343240 AT5G37670.1 36.792 106 58 4 125 221 28 133 7.15e-12 61.2
Msa0343240 AT1G53540.1 32.673 101 65 2 124 221 55 155 1.97e-11 60.5
Msa0343240 AT1G54050.1 34.188 117 62 4 104 206 22 137 4.99e-11 59.3
Msa0343240 AT1G54050.2 34.188 117 62 4 104 206 22 137 4.99e-11 59.3
Msa0343240 AT1G59860.1 34.951 103 60 4 117 215 46 145 5.41e-11 59.3

Find 63 sgRNAs with CRISPR-Local

Find 67 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTTCATCATTCTTCTTCT+TGG 0.080906 3_1:-1241953 None:intergenic
TTCTCAAACTGCACATTCTT+TGG 0.245312 3_1:-1242024 None:intergenic
CTGCTGCACCGATAGGGTTA+TGG 0.285020 3_1:+1241656 Msa0343240:CDS
AAGGAGTAGGTGGGTATAGA+AGG 0.294040 3_1:+1241794 Msa0343240:CDS
ACAAAGAGGATGTTAAAGTT+TGG 0.296691 3_1:+1241890 Msa0343240:CDS
CACTCTTTATCCTTAAATCT+TGG 0.324147 3_1:+1241478 Msa0343240:CDS
TGTGCAGTTTGAGAATATTA+AGG 0.336710 3_1:+1242032 Msa0343240:CDS
TGCTGCACCGATAGGGTTAT+GGG 0.365142 3_1:+1241657 Msa0343240:CDS
CAAAGAGGATGTTAAAGTTT+GGG 0.367241 3_1:+1241891 Msa0343240:CDS
TTCTCTTGCTTCACCTGTCA+TGG 0.368553 3_1:-1241563 None:intergenic
GAATATTAAGGCTGAGGTTA+AGG 0.368713 3_1:+1242044 Msa0343240:CDS
AACATTAAGATCAAGGACTT+TGG 0.390389 3_1:-1242112 None:intergenic
CAGGTTAAGACCAAGATTTA+AGG 0.391199 3_1:-1241488 None:intergenic
AGTTTGTCTTGATGAACTCT+TGG 0.419007 3_1:-1241521 None:intergenic
TGATGAACTCTTGGAAATCA+TGG 0.438584 3_1:-1241512 None:intergenic
AAGGACAGTGCAAGAGATGA+TGG 0.443408 3_1:+1241696 Msa0343240:CDS
GTCATTGAACATTAAGATCA+AGG 0.444311 3_1:-1242119 None:intergenic
TCTCCTTCTTTAATCTCCCA+TGG 0.457000 3_1:-1241829 None:intergenic
ATTAAGGCTGAGGTTAAGGA+TGG 0.462230 3_1:+1242048 Msa0343240:CDS
TCATCGCCGCTACCGATTGA+AGG 0.462817 3_1:+1241775 Msa0343240:CDS
CTCTCTTCTTGGGTTCGGAC+TGG 0.469722 3_1:-1241627 None:intergenic
TCAATTGTACTCATTGCAAA+CGG 0.479546 3_1:-1241745 None:intergenic
CATAACCCTATCGGTGCAGC+AGG 0.490168 3_1:-1241655 None:intergenic
GAATAAGTAGTAGTAGCCTT+AGG 0.490783 3_1:-1242090 None:intergenic
CAATTGTACTCATTGCAAAC+GGG 0.496144 3_1:-1241744 None:intergenic
TCAATCGGTAGCGGCGATGA+AGG 0.505690 3_1:-1241772 None:intergenic
CTTCGAGTCAAAGCCATGAC+AGG 0.510747 3_1:+1241550 Msa0343240:CDS
GTTTGAGAATATTAAGGCTG+AGG 0.515148 3_1:+1242038 Msa0343240:CDS
AGAATGATGAAGATGAAGAA+TGG 0.516116 3_1:+1241962 Msa0343240:CDS
TCTTTACATAACCATTCCTA+AGG 0.516609 3_1:+1242074 Msa0343240:CDS
AGTAGGTGGGTATAGAAGGA+GGG 0.522121 3_1:+1241798 Msa0343240:CDS
ACATCCTCTTTGTTCATACC+AGG 0.528873 3_1:-1241880 None:intergenic
GAGTAGGTGGGTATAGAAGG+AGG 0.533601 3_1:+1241797 Msa0343240:CDS
CTGTCATGGCTTTGACTCGA+AGG 0.534564 3_1:-1241549 None:intergenic
TACCCACCTACTCCTTCAAT+CGG 0.537111 3_1:-1241787 None:intergenic
AACTCTTGGAAATCATGGAC+AGG 0.539369 3_1:-1241507 None:intergenic
TAAGGCTGAGGTTAAGGATG+GGG 0.545488 3_1:+1242050 Msa0343240:CDS
ACACCATGGGAGATTAAAGA+AGG 0.546168 3_1:+1241826 Msa0343240:CDS
GTAGGTGGGTATAGAAGGAG+GGG 0.548522 3_1:+1241799 Msa0343240:CDS
GAGAAAGTGAATACAAGATG+AGG 0.559520 3_1:+1241848 Msa0343240:CDS
GAAGAATGGTCTAAGAGCTA+TGG 0.561468 3_1:+1241976 Msa0343240:CDS
AAGATGAGGTTTGATATGCC+TGG 0.574216 3_1:+1241862 Msa0343240:CDS
TTGGGTGGAAGAGAAGATGT+TGG 0.576341 3_1:+1241909 Msa0343240:CDS
CCGCTACCGATTGAAGGAGT+AGG 0.578817 3_1:+1241781 Msa0343240:CDS
AGAGGATGTTAAAGTTTGGG+TGG 0.581219 3_1:+1241894 Msa0343240:CDS
TCTTGCACTGTCCTTGTCGT+AGG 0.581285 3_1:-1241688 None:intergenic
GGGAACAATGGAAAGCATGA+TGG 0.589950 3_1:+1241717 Msa0343240:CDS
TCTTCTTGGGTTCGGACTGG+CGG 0.591487 3_1:-1241624 None:intergenic
TTAAGGCTGAGGTTAAGGAT+GGG 0.596993 3_1:+1242049 Msa0343240:CDS
GCAAGAGATGATGGGAACAA+TGG 0.598164 3_1:+1241705 Msa0343240:CDS
AATCGATCCCATAACCCTAT+CGG 0.598439 3_1:-1241664 None:intergenic
AGGACAGTGCAAGAGATGAT+GGG 0.601662 3_1:+1241697 Msa0343240:CDS
TATTGTTTGATCTTGAAACA+TGG 0.608054 3_1:-1241594 None:intergenic
AGTAGTAGTAGCCTTAGGAA+TGG 0.626005 3_1:-1242085 None:intergenic
TTGCAATGAGTACAATTGAG+TGG 0.632366 3_1:+1241749 Msa0343240:CDS
CTACCGATTGAAGGAGTAGG+TGG 0.639864 3_1:+1241784 Msa0343240:CDS
AAGGAGGGGAAGAACACCAT+GGG 0.642482 3_1:+1241813 Msa0343240:CDS
AATGGTCTAAGAGCTATGGT+AGG 0.662268 3_1:+1241980 Msa0343240:CDS
CCTACTCCTTCAATCGGTAG+CGG 0.682306 3_1:-1241781 None:intergenic
TACCGATTGAAGGAGTAGGT+GGG 0.683545 3_1:+1241785 Msa0343240:CDS
GAAGGAGGGGAAGAACACCA+TGG 0.687857 3_1:+1241812 Msa0343240:CDS
GGGATCGATTTCCTACGACA+AGG 0.701576 3_1:+1241677 Msa0343240:CDS
TATGCCTGGTATGAACAAAG+AGG 0.732695 3_1:+1241876 Msa0343240:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TATTGTTTGATCTTGAAACA+TGG - chr3_1:1241597-1241616 None:intergenic 25.0%
CACTCTTTATCCTTAAATCT+TGG + chr3_1:1241478-1241497 Msa0343240:CDS 30.0%
TCAATTGTACTCATTGCAAA+CGG - chr3_1:1241748-1241767 None:intergenic 30.0%
ACAAAGAGGATGTTAAAGTT+TGG + chr3_1:1241890-1241909 Msa0343240:CDS 30.0%
CAAAGAGGATGTTAAAGTTT+GGG + chr3_1:1241891-1241910 Msa0343240:CDS 30.0%
ATCTTCATCATTCTTCTTCT+TGG - chr3_1:1241956-1241975 None:intergenic 30.0%
AGAATGATGAAGATGAAGAA+TGG + chr3_1:1241962-1241981 Msa0343240:CDS 30.0%
TGTGCAGTTTGAGAATATTA+AGG + chr3_1:1242032-1242051 Msa0343240:CDS 30.0%
TCTTTACATAACCATTCCTA+AGG + chr3_1:1242074-1242093 Msa0343240:CDS 30.0%
AACATTAAGATCAAGGACTT+TGG - chr3_1:1242115-1242134 None:intergenic 30.0%
CAGGTTAAGACCAAGATTTA+AGG - chr3_1:1241491-1241510 None:intergenic 35.0%
TGATGAACTCTTGGAAATCA+TGG - chr3_1:1241515-1241534 None:intergenic 35.0%
AGTTTGTCTTGATGAACTCT+TGG - chr3_1:1241524-1241543 None:intergenic 35.0%
AAAAACTCTCTTCTTGGGTT+CGG - chr3_1:1241635-1241654 None:intergenic 35.0%
CAATTGTACTCATTGCAAAC+GGG - chr3_1:1241747-1241766 None:intergenic 35.0%
TTGCAATGAGTACAATTGAG+TGG + chr3_1:1241749-1241768 Msa0343240:CDS 35.0%
GAGAAAGTGAATACAAGATG+AGG + chr3_1:1241848-1241867 Msa0343240:CDS 35.0%
TTCTCAAACTGCACATTCTT+TGG - chr3_1:1242027-1242046 None:intergenic 35.0%
GTTTGAGAATATTAAGGCTG+AGG + chr3_1:1242038-1242057 Msa0343240:CDS 35.0%
GAATATTAAGGCTGAGGTTA+AGG + chr3_1:1242044-1242063 Msa0343240:CDS 35.0%
GAATAAGTAGTAGTAGCCTT+AGG - chr3_1:1242093-1242112 None:intergenic 35.0%
AACTCTTGGAAATCATGGAC+AGG - chr3_1:1241510-1241529 None:intergenic 40.0%
GCAGGAAAAACTCTCTTCTT+GGG - chr3_1:1241640-1241659 None:intergenic 40.0%
AGCAGGAAAAACTCTCTTCT+TGG - chr3_1:1241641-1241660 None:intergenic 40.0%
AATCGATCCCATAACCCTAT+CGG - chr3_1:1241667-1241686 None:intergenic 40.0%
ACACCATGGGAGATTAAAGA+AGG + chr3_1:1241826-1241845 Msa0343240:CDS 40.0%
TCTCCTTCTTTAATCTCCCA+TGG - chr3_1:1241832-1241851 None:intergenic 40.0%
AAGATGAGGTTTGATATGCC+TGG + chr3_1:1241862-1241881 Msa0343240:CDS 40.0%
TATGCCTGGTATGAACAAAG+AGG + chr3_1:1241876-1241895 Msa0343240:CDS 40.0%
ACATCCTCTTTGTTCATACC+AGG - chr3_1:1241883-1241902 None:intergenic 40.0%
AGAGGATGTTAAAGTTTGGG+TGG + chr3_1:1241894-1241913 Msa0343240:CDS 40.0%
GAAGAATGGTCTAAGAGCTA+TGG + chr3_1:1241976-1241995 Msa0343240:CDS 40.0%
AATGGTCTAAGAGCTATGGT+AGG + chr3_1:1241980-1241999 Msa0343240:CDS 40.0%
ATTAAGGCTGAGGTTAAGGA+TGG + chr3_1:1242048-1242067 Msa0343240:CDS 40.0%
TTAAGGCTGAGGTTAAGGAT+GGG + chr3_1:1242049-1242068 Msa0343240:CDS 40.0%
! AGTAGTAGTAGCCTTAGGAA+TGG - chr3_1:1242088-1242107 None:intergenic 40.0%
TTCTCTTGCTTCACCTGTCA+TGG - chr3_1:1241566-1241585 None:intergenic 45.0%
!! TTTTTCCTGCTGCACCGATA+GGG + chr3_1:1241650-1241669 Msa0343240:CDS 45.0%
AAGGACAGTGCAAGAGATGA+TGG + chr3_1:1241696-1241715 Msa0343240:CDS 45.0%
AGGACAGTGCAAGAGATGAT+GGG + chr3_1:1241697-1241716 Msa0343240:CDS 45.0%
!! GCAAGAGATGATGGGAACAA+TGG + chr3_1:1241705-1241724 Msa0343240:CDS 45.0%
! GGGAACAATGGAAAGCATGA+TGG + chr3_1:1241717-1241736 Msa0343240:CDS 45.0%
! TACCGATTGAAGGAGTAGGT+GGG + chr3_1:1241785-1241804 Msa0343240:CDS 45.0%
TACCCACCTACTCCTTCAAT+CGG - chr3_1:1241790-1241809 None:intergenic 45.0%
AAGGAGTAGGTGGGTATAGA+AGG + chr3_1:1241794-1241813 Msa0343240:CDS 45.0%
AGTAGGTGGGTATAGAAGGA+GGG + chr3_1:1241798-1241817 Msa0343240:CDS 45.0%
TTGGGTGGAAGAGAAGATGT+TGG + chr3_1:1241909-1241928 Msa0343240:CDS 45.0%
TAAGGCTGAGGTTAAGGATG+GGG + chr3_1:1242050-1242069 Msa0343240:CDS 45.0%
!! CTGTCATGGCTTTGACTCGA+AGG - chr3_1:1241552-1241571 None:intergenic 50.0%
CTTCGAGTCAAAGCCATGAC+AGG + chr3_1:1241550-1241569 Msa0343240:CDS 50.0%
!! GTTTTTCCTGCTGCACCGAT+AGG + chr3_1:1241649-1241668 Msa0343240:CDS 50.0%
! TGCTGCACCGATAGGGTTAT+GGG + chr3_1:1241657-1241676 Msa0343240:CDS 50.0%
! GGGATCGATTTCCTACGACA+AGG + chr3_1:1241677-1241696 Msa0343240:CDS 50.0%
TCTTGCACTGTCCTTGTCGT+AGG - chr3_1:1241691-1241710 None:intergenic 50.0%
CCTACTCCTTCAATCGGTAG+CGG - chr3_1:1241784-1241803 None:intergenic 50.0%
! CTACCGATTGAAGGAGTAGG+TGG + chr3_1:1241784-1241803 Msa0343240:CDS 50.0%
GAGTAGGTGGGTATAGAAGG+AGG + chr3_1:1241797-1241816 Msa0343240:CDS 50.0%
GTAGGTGGGTATAGAAGGAG+GGG + chr3_1:1241799-1241818 Msa0343240:CDS 50.0%
AAGGAGGGGAAGAACACCAT+GGG + chr3_1:1241813-1241832 Msa0343240:CDS 50.0%
! TCTTCTTGGGTTCGGACTGG+CGG - chr3_1:1241627-1241646 None:intergenic 55.0%
CTCTCTTCTTGGGTTCGGAC+TGG - chr3_1:1241630-1241649 None:intergenic 55.0%
CATAACCCTATCGGTGCAGC+AGG - chr3_1:1241658-1241677 None:intergenic 55.0%
! CTGCTGCACCGATAGGGTTA+TGG + chr3_1:1241656-1241675 Msa0343240:CDS 55.0%
! TCAATCGGTAGCGGCGATGA+AGG - chr3_1:1241775-1241794 None:intergenic 55.0%
! TCATCGCCGCTACCGATTGA+AGG + chr3_1:1241775-1241794 Msa0343240:CDS 55.0%
! CCGCTACCGATTGAAGGAGT+AGG + chr3_1:1241781-1241800 Msa0343240:CDS 55.0%
GAAGGAGGGGAAGAACACCA+TGG + chr3_1:1241812-1241831 Msa0343240:CDS 55.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 1241472 1242140 1241472 ID=Msa0343240;Name=Msa0343240
chr3_1 mRNA 1241472 1242140 1241472 ID=Msa0343240-mRNA-1;Parent=Msa0343240;Name=Msa0343240-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|222
chr3_1 exon 1241472 1242140 1241472 ID=Msa0343240-mRNA-1:exon:383;Parent=Msa0343240-mRNA-1
chr3_1 CDS 1241472 1242140 1241472 ID=Msa0343240-mRNA-1:cds;Parent=Msa0343240-mRNA-1
Gene Sequence

>Msa0343240

ATGGCACACTCTTTATCCTTAAATCTTGGTCTTAACCTGTCCATGATTTCCAAGAGTTCATCAAGACAAACTCATTTCCTTCGAGTCAAAGCCATGACAGGTGAAGCAAGAGAAAAACTTGACCATGTTTCAAGATCAAACAATATTAAACACCGCCAGTCCGAACCCAAGAAGAGAGTTTTTCCTGCTGCACCGATAGGGTTATGGGATCGATTTCCTACGACAAGGACAGTGCAAGAGATGATGGGAACAATGGAAAGCATGATGGAAGACCCGTTTGCAATGAGTACAATTGAGTGGCCTTCATCGCCGCTACCGATTGAAGGAGTAGGTGGGTATAGAAGGAGGGGAAGAACACCATGGGAGATTAAAGAAGGAGAAAGTGAATACAAGATGAGGTTTGATATGCCTGGTATGAACAAAGAGGATGTTAAAGTTTGGGTGGAAGAGAAGATGTTGGTTGTGAAAGCTGAGAAAGCATCCAAGAAGAAGAATGATGAAGATGAAGAATGGTCTAAGAGCTATGGTAGGTATAGTAGCAGAATTGCTTTGCCAAAGAATGTGCAGTTTGAGAATATTAAGGCTGAGGTTAAGGATGGGGTTCTTTACATAACCATTCCTAAGGCTACTACTACTTATTCCAAAGTCCTTGATCTTAATGTTCAATGA

Protein sequence

>Msa0343240

MAHSLSLNLGLNLSMISKSSSRQTHFLRVKAMTGEAREKLDHVSRSNNIKHRQSEPKKRVFPAAPIGLWDRFPTTRTVQEMMGTMESMMEDPFAMSTIEWPSSPLPIEGVGGYRRRGRTPWEIKEGESEYKMRFDMPGMNKEDVKVWVEEKMLVVKAEKASKKKNDEDEEWSKSYGRYSSRIALPKNVQFENIKAEVKDGVLYITIPKATTTYSKVLDLNVQ*