AlfalfaGEDB | Msa0274040

M. sativa cultivar zhongmu-4 / Msa0274040

KEGG: -
GO: GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009534 GO:0009536 GO:0009579 GO:0030312 GO:0031976 GO:0031984 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048046 GO:0071944
PFAM: PF00657.25

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0274040	KEH36666.1	93.696	349	22	0	1	349	1	349	0.0	682

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0274040	sp\|Q9C7N4\|GDL15_ARATH	44.262	366	172	8	1	338	1	362	1.99e-100	303

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0274040	A0A072V4S0	93.696	349	22	0	1	349	1	349	0.0	682

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0015930	Msa0274040	-0.805630	1.216402e-49	-8.615850e-47
Msa0020390	Msa0274040	-0.861180	1.229759e-63	-8.615850e-47
Msa0038870	Msa0274040	-0.812172	4.845696e-51	-8.615850e-47
Msa0044000	Msa0274040	-0.820926	5.297498e-53	-8.615850e-47
Msa0115190	Msa0274040	-0.831805	1.355770e-55	-8.615850e-47
Msa0118540	Msa0274040	-0.829101	6.215919e-55	-8.615850e-47
Msa0127800	Msa0274040	-0.813654	2.294160e-51	-8.615850e-47
Msa0149440	Msa0274040	-0.801710	7.919477e-49	-8.615850e-47
Msa0159420	Msa0274040	-0.813287	2.763086e-51	-8.615850e-47
Msa0162130	Msa0274040	-0.833342	5.635351e-56	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0274040	MtrunA17_Chr2g0284941	93.696	349	22	0	1	349	1	349	0.0	682
Msa0274040	MtrunA17_Chr2g0285001	76.819	371	64	3	1	349	1	371	0.0	567
Msa0274040	MtrunA17_Chr2g0284951	77.297	370	58	4	1	349	1	365	0.0	566
Msa0274040	MtrunA17_Chr2g0284991	74.114	367	74	2	1	346	1	367	0.0	559
Msa0274040	MtrunA17_Chr2g0284921	58.146	356	125	5	6	338	6	360	1.38e-133	385
Msa0274040	MtrunA17_Chr3g0085881	55.493	355	138	4	1	338	1	352	1.68e-127	369
Msa0274040	MtrunA17_Chr8g0380031	55.432	359	130	7	1	338	56	405	1.13e-124	364
Msa0274040	MtrunA17_Chr8g0380041	44.687	367	167	9	1	338	3	362	4.26e-99	297
Msa0274040	MtrunA17_Chr3g0086641	44.898	343	157	7	25	338	30	369	2.09e-94	285
Msa0274040	MtrunA17_Chr2g0285061	44.248	339	158	7	29	338	28	364	9.99e-93	281

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0274040	AT1G29670.1	44.262	366	172	8	1	338	1	362	2.03e-101	303
Msa0274040	AT1G29670.2	44.262	366	172	8	1	338	69	430	3.25e-100	303
Msa0274040	AT1G29660.1	43.052	367	176	7	1	338	1	363	2.31e-98	296
Msa0274040	AT4G18970.1	44.282	341	159	7	27	338	22	360	3.46e-93	282
Msa0274040	AT5G45670.1	44.643	336	155	7	31	337	27	360	6.14e-93	282
Msa0274040	AT4G18970.2	44.282	341	159	7	27	338	22	360	4.10e-92	281
Msa0274040	AT1G33811.1	40.233	343	169	6	28	337	29	368	7.61e-85	261
Msa0274040	AT2G19010.1	41.543	337	160	6	8	320	10	333	1.66e-81	252
Msa0274040	AT2G19010.2	41.543	337	160	6	8	320	40	363	1.25e-80	251
Msa0274040	AT2G19050.1	41.720	314	151	6	30	319	28	333	3.02e-74	233

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Find 85 sgRNAs with CRISPR-Local

Find 216 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATATGTGTTAGTTGGGT+TGG	0.414588	2_3:+5798956	Msa0274040:CDS
AAAATTATTACCATCTGACA+GGG	0.687534	2_3:-5799102	None:intergenic
AAAGGTGTGAATTATGCATC+TGG	0.501240	2_3:+5798439	Msa0274040:CDS
AAATAGAATCAAACGTAAGG+TGG	0.643932	2_3:-5799614	None:intergenic
AAATATGTGTTAGTTGGGTT+GGG	0.439264	2_3:+5798957	Msa0274040:CDS
AAATGTAATTACAAACCTTA+TGG	0.329477	2_3:+5798200	Msa0274040:CDS
AAGTCCATCCCACCGAGGCT+TGG	0.557254	2_3:+5799334	Msa0274040:CDS
AATCTTCCGGTTGGGCCCAT+CGG	0.538489	2_3:-5798233	None:intergenic
AATGAGAAGGTACCAAGCCT+CGG	0.626887	2_3:-5799346	None:intergenic
AATGGAGACATACTCGGCTA+AGG	0.563484	2_3:+5798477	Msa0274040:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTAGACTTTCTTCTTAGAGA+TGG	+	chr2_3:5798019-5798038	Msa0274040:exon	30.0%
TGGATTCTATAATCAAGACA+TGG	+	chr2_3:5798039-5798058	Msa0274040:CDS	30.0%
TTCTATAATCAAGACATGGT+TGG	+	chr2_3:5798043-5798062	Msa0274040:CDS	30.0%
TTTGCAGACAAAAGAAGAAT+AGG	-	chr2_3:5798074-5798093	None:intergenic	30.0%
TCTCCAAAGATAAATACACA+AGG	-	chr2_3:5798134-5798153	None:intergenic	30.0%
AAATAACATTATGTCCGTAC+AGG	+	chr2_3:5798564-5798583	Msa0274040:intron	30.0%
AATAACATTATGTCCGTACA+GGG	+	chr2_3:5798565-5798584	Msa0274040:intron	30.0%
CAATTAGTGAAAGAAAGCAA+GGG	-	chr2_3:5798866-5798885	None:intergenic	30.0%
TCAATTAGTGAAAGAAAGCA+AGG	-	chr2_3:5798867-5798886	None:intergenic	30.0%
TATTAGTTCCATTTGATTCC+AGG	+	chr2_3:5798911-5798930	Msa0274040:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_3	gene	5797989	5799708	5797989	ID=Msa0274040;Name=Msa0274040
chr2_3	mRNA	5797989	5799708	5797989	ID=Msa0274040-mRNA-1;Parent=Msa0274040;Name=Msa0274040-mRNA-1;_AED=0.23;_eAED=0.23;_QI=49\|1\|1\|1\|1\|1\|5\|232\|349
chr2_3	exon	5797989	5798287	5797989	ID=Msa0274040-mRNA-1:exon:2709;Parent=Msa0274040-mRNA-1
chr2_3	exon	5798374	5798498	5798374	ID=Msa0274040-mRNA-1:exon:2710;Parent=Msa0274040-mRNA-1
chr2_3	exon	5798586	5798831	5798586	ID=Msa0274040-mRNA-1:exon:2711;Parent=Msa0274040-mRNA-1
chr2_3	exon	5798933	5799113	5798933	ID=Msa0274040-mRNA-1:exon:2712;Parent=Msa0274040-mRNA-1
chr2_3	exon	5799229	5799708	5799229	ID=Msa0274040-mRNA-1:exon:2713;Parent=Msa0274040-mRNA-1
chr2_3	five_prime_UTR	5797989	5798037	5797989	ID=Msa0274040-mRNA-1:five_prime_utr;Parent=Msa0274040-mRNA-1
chr2_3	CDS	5798038	5798287	5798038	ID=Msa0274040-mRNA-1:cds;Parent=Msa0274040-mRNA-1
chr2_3	CDS	5798374	5798498	5798374	ID=Msa0274040-mRNA-1:cds;Parent=Msa0274040-mRNA-1
chr2_3	CDS	5798586	5798831	5798586	ID=Msa0274040-mRNA-1:cds;Parent=Msa0274040-mRNA-1
chr2_3	CDS	5798933	5799113	5798933	ID=Msa0274040-mRNA-1:cds;Parent=Msa0274040-mRNA-1
chr2_3	CDS	5799229	5799476	5799229	ID=Msa0274040-mRNA-1:cds;Parent=Msa0274040-mRNA-1
chr2_3	three_prime_UTR	5799477	5799708	5799477	ID=Msa0274040-mRNA-1:three_prime_utr;Parent=Msa0274040-mRNA-1

Gene Sequence

>Msa0274040

ATGGATTCTATAATCAAGACATGGTTGGTGTTACCTATTCTTCTTTTGTCTGCAAACTGTTTGCAACGATGTGTCAATGGGAAATCCCAAGTGCCTTGTGTATTTATCTTTGGAGACTCTTTATCTGATAGTGGAAACAACAATAACCTTCCTACCTCTGCAAAATGTAATTACAAACCTTATGGAGTTGACTTTCCGATGGGCCCAACCGGAAGATTTACCAACGGTCGAACTGCAGTTGACATAATAACTCAACTTCTTGGATTTGAGAATTTCATTCCACCATTTGCAAACATCAGTGGTTCAGACATACTCAAAGGTGTGAATTATGCATCTGGCGGAGCTGGAATTCGAATGGAGACATACTCGGCTAAGGGTTATGCTATTAGCTTGGGATTACAGTTAAGAAATCACAGAGTTATAGTTTCTCAAATTGCTAGCAGACTTGGAGGTATTGACAAAGCTCAAGAATACCTTAACAAATGCTTGTATTATGTGAATATTGGAAGTAACGATTACATAAACAATTACTTTCTTCCCCAATTCTACCCAACAAGTCATACTTATAGCCCTGAGCAGTATGCAGAAGCTCTAATTCAAGAGTTATCTTTGAATTTACTGGCTCTACATGACATTGGAGCGAGAAAATATGTGTTAGTTGGGTTGGGACTTTCAAGCAGTACTCCGTCACTTTTCAATTACAAGCTTAAATCTCTAGTGGAACATTTCAATAATAAGTTCTCTGCTGATTCTAAATTCATATTTATAAACACTACATTAGAGTCAGATGCCCTGTCAGATGGTTTTTTGGTTTCAAATGCTCCTTGTTGTCCATCAAGGTTGAATGGATTGTGCATTCCTGATGAAAAGCCATGCTATAATAGAAGTGACTATGCGTTTTGGGATGAAGTCCATCCCACCGAGGCTTGGTACCTTCTCATTGCAACAAGAATGTATGATTCATCTAATAATTCAGGGTTCACTTATCCAATGGATATTAAGCACCTTGTTGAACAAGAAATTGATATGGAATTGGATTTCACTTCATAG

Protein sequence

>Msa0274040

MDSIIKTWLVLPILLLSANCLQRCVNGKSQVPCVFIFGDSLSDSGNNNNLPTSAKCNYKPYGVDFPMGPTGRFTNGRTAVDIITQLLGFENFIPPFANISGSDILKGVNYASGGAGIRMETYSAKGYAISLGLQLRNHRVIVSQIASRLGGIDKAQEYLNKCLYYVNIGSNDYINNYFLPQFYPTSHTYSPEQYAEALIQELSLNLLALHDIGARKYVLVGLGLSSSTPSLFNYKLKSLVEHFNNKFSADSKFIFINTTLESDALSDGFLVSNAPCCPSRLNGLCIPDEKPCYNRSDYAFWDEVHPTEAWYLLIATRMYDSSNNSGFTYPMDIKHLVEQEIDMELDFTS*