AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0696130

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0696130 MtrunA17_Chr4g0048131 96.410 195 7 0 1 195 1 195 6.01e-130 363
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0696130 AT1G11240.1 48.428 159 76 3 14 170 21 175 9.20e-29 106

Find 57 sgRNAs with CRISPR-Local

Find 219 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTTGTCAGTTTCTTCTTCT+TGG 0.106094 4_4:-65974268 None:intergenic
CTTCTTCATCCATGGTTTCC+AGG 0.197486 4_4:-65973890 None:intergenic
AATGGCTTCTTGTTATTTAT+AGG 0.201940 4_4:-65975682 None:intergenic
CCTTGTTTAGCTGATCCATT+TGG 0.249463 4_4:+65976239 Msa0696130:three_prime_UTR
GATCTCAAAGATTATGTTAC+TGG 0.251622 4_4:+65973996 Msa0696130:CDS
CTTGTTTAGCTGATCCATTT+GGG 0.261420 4_4:+65976240 Msa0696130:three_prime_UTR
AGCTTCTTCTTGTTGTTTGT+TGG 0.267711 4_4:-65974054 None:intergenic
TAACCATTCACACAGAATTA+AGG 0.273952 4_4:-65976488 None:intergenic
TAACAAGAAGCCATTTAAAA+AGG 0.309156 4_4:+65975690 Msa0696130:CDS
TTGGAAAGAGAACTTGCTTA+TGG 0.326372 4_4:+65974197 Msa0696130:CDS
TCTCTCTTACTTCTTTGCTT+TGG 0.342431 4_4:-65975736 None:intergenic
AGGTACCGTCACTTGTTTCC+CGG 0.345185 4_4:-65975778 None:intergenic
GCAACAAACTTTATTCTTTG+TGG 0.352202 4_4:+65975835 Msa0696130:three_prime_UTR
CTAGGGAAACTCTCTTCGTC+AGG 0.359375 4_4:-65975592 None:intergenic
GGGGCTTCTTTCCTCTTGCT+AGG 0.380831 4_4:-65975610 None:intergenic
ACCTTGGCATATTGCTTCTT+CGG 0.382775 4_4:+65975797 Msa0696130:three_prime_UTR
ACGCAACGAGGAACGCATAA+AGG 0.384606 4_4:+65974088 Msa0696130:CDS
TGGAAAGAGAACTTGCTTAT+GGG 0.391137 4_4:+65974198 Msa0696130:CDS
CCAACTGATGACACTGAAAC+TGG 0.412428 4_4:+65974230 Msa0696130:CDS
TTCTTCATCCATGGTTTCCA+GGG 0.414232 4_4:-65973889 None:intergenic
GCAATATCAGAGCGCAAAAC+TGG 0.417440 4_4:+65973915 Msa0696130:CDS
AATAAAGTTTGTTGCAGACT+TGG 0.422058 4_4:-65975827 None:intergenic
AAACAAGTTCAATCTATTGC+TGG 0.433583 4_4:+65974287 Msa0696130:CDS
TATTGCTGCTTCTTCATCCA+TGG 0.449284 4_4:-65973898 None:intergenic
CCAGTTTCAGTGTCATCAGT+TGG 0.464675 4_4:-65974230 None:intergenic
ATGGGAGGACACTGCTGGTG+GGG 0.467882 4_4:-65975629 None:intergenic
GGGCTTCTTTCCTCTTGCTA+GGG 0.473502 4_4:-65975609 None:intergenic
ATAAAGTTTGTTGCAGACTT+GGG 0.503632 4_4:-65975826 None:intergenic
AAAAGGAAAGGAAAGAAGCC+GGG 0.511053 4_4:+65975760 Msa0696130:CDS
AATTTCAGTGTCTCTCAAGC+TGG 0.511341 4_4:+65976395 Msa0696130:three_prime_UTR
TTTGTACACGGGTGAGTCGT+AGG 0.513879 4_4:+65973845 Msa0696130:five_prime_UTR
GATGGGAGGACACTGCTGGT+GGG 0.523396 4_4:-65975630 None:intergenic
GGAAAGAGAACTTGCTTATG+GGG 0.538034 4_4:+65974199 Msa0696130:CDS
TTTATCAGCTACAACAGGAT+GGG 0.538526 4_4:-65975647 None:intergenic
GGATGGGAGGACACTGCTGG+TGG 0.539822 4_4:-65975631 None:intergenic
TTGTTTAGCTGATCCATTTG+GGG 0.542308 4_4:+65976241 Msa0696130:three_prime_UTR
GGAAACAAGTGACGGTACCT+TGG 0.547866 4_4:+65975781 Msa0696130:exon
CCAAATGGATCAGCTAAACA+AGG 0.550531 4_4:-65976239 None:intergenic
CGGCTGAACCCTGGAAACCA+TGG 0.569330 4_4:+65973881 Msa0696130:exon
GGTTGATTGCGGCTGAACCC+TGG 0.571211 4_4:+65973872 Msa0696130:five_prime_UTR
CACGGGTGAGTCGTAGGTTG+TGG 0.574316 4_4:+65973851 Msa0696130:five_prime_UTR
TGTTTAGCTGATCCATTTGG+GGG 0.574468 4_4:+65976242 Msa0696130:three_prime_UTR
AAGAGAGTTTCCCTAGCAAG+AGG 0.580202 4_4:+65975599 Msa0696130:CDS
ACAGGATGGGAGGACACTGC+TGG 0.582988 4_4:-65975634 None:intergenic
ACAAACTTTATTCTTTGTGG+AGG 0.583529 4_4:+65975838 Msa0696130:three_prime_UTR
ATCTTTCTCATCGAAAGAGA+TGG 0.597691 4_4:-65973976 None:intergenic
ATCAGCTACAACAGGATGGG+AGG 0.599440 4_4:-65975644 None:intergenic
TCGTAGGTTGTGGTTGATTG+CGG 0.603683 4_4:+65973861 Msa0696130:five_prime_UTR
GTTTAGCTGATCCATTTGGG+GGG 0.609456 4_4:+65976243 Msa0696130:three_prime_UTR
TAGGGAAACTCTCTTCGTCA+GGG 0.612538 4_4:-65975591 None:intergenic
GTTTCAGTGTCATCAGTTGG+TGG 0.621072 4_4:-65974227 None:intergenic
TCCGAAGAAGCAATATGCCA+AGG 0.646516 4_4:-65975798 None:intergenic
CATTAGAAGATCACATACAA+AGG 0.648568 4_4:-65976457 None:intergenic
TTTAAGACGCAAACGCAACG+AGG 0.676281 4_4:+65974076 Msa0696130:CDS
CAAATGGATCAGCTAAACAA+GGG 0.679981 4_4:-65976238 None:intergenic
GAAAGAGAACTTGCTTATGG+GGG 0.681530 4_4:+65974200 Msa0696130:CDS
AGAAGCCGGGAAACAAGTGA+CGG 0.722721 4_4:+65975773 Msa0696130:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTATTTTTAGACAAAAAAT+AGG + chr4_4:65973816-65973835 Msa0696130:five_prime_UTR 10.0%
!!! AAGAAAAGTGTATAAATAAA+AGG + chr4_4:65975939-65975958 Msa0696130:intron 15.0%
!!! AGAAAAGTGTATAAATAAAA+GGG + chr4_4:65975940-65975959 Msa0696130:intron 15.0%
!! TATTTAGTGTTATATTGTAT+TGG + chr4_4:65976003-65976022 Msa0696130:intron 15.0%
!! ATTTAGTGTTATATTGTATT+GGG + chr4_4:65976004-65976023 Msa0696130:intron 15.0%
!!! ATTTTTAGACAAAAAATAGG+AGG + chr4_4:65973819-65973838 Msa0696130:five_prime_UTR 20.0%
!!! TTTTTAGACAAAAAATAGGA+GGG + chr4_4:65973820-65973839 Msa0696130:five_prime_UTR 20.0%
!!! AAAATTCAATTTTATTGCAG+AGG + chr4_4:65974171-65974190 Msa0696130:intron 20.0%
!!! TAAGCTATTTCTATAACATA+AGG + chr4_4:65974406-65974425 Msa0696130:intron 20.0%
!! GAAACAAATTGACAAATTAA+GGG - chr4_4:65974645-65974664 None:intergenic 20.0%
!! AGAAACAAATTGACAAATTA+AGG - chr4_4:65974646-65974665 None:intergenic 20.0%
!! CAAAGTAAGTCAATTAAATT+GGG - chr4_4:65975903-65975922 None:intergenic 20.0%
!! AAAGTGGAATATATTGTATT+GGG + chr4_4:65975963-65975982 Msa0696130:intron 20.0%
!! GTTGTATTGAAAATTGAAAT+TGG + chr4_4:65976037-65976056 Msa0696130:intron 20.0%
!!! GACACTTTTTTTTAGAAAAT+GGG + chr4_4:65976073-65976092 Msa0696130:intron 20.0%
!! GAATCTTTAAGAAAATGAGT+AGG - chr4_4:65974116-65974135 None:intergenic 25.0%
!!! GCTATTTGGTTTAATTTTTC+TGG + chr4_4:65975435-65975454 Msa0696130:intron 25.0%
!! ATCATTTTCATATGTCTTTG+TGG - chr4_4:65975536-65975555 None:intergenic 25.0%
!!! AATGGCTTCTTGTTATTTAT+AGG - chr4_4:65975685-65975704 None:intergenic 25.0%
! TAACAAGAAGCCATTTAAAA+AGG + chr4_4:65975690-65975709 Msa0696130:CDS 25.0%
! AAAGAAGTAAGAGAGAAAAA+AGG + chr4_4:65975743-65975762 Msa0696130:CDS 25.0%
! CCAAAGTAAGTCAATTAAAT+TGG - chr4_4:65975904-65975923 None:intergenic 25.0%
!! CCAATTTAATTGACTTACTT+TGG + chr4_4:65975901-65975920 Msa0696130:intron 25.0%
! TGTATAAATAAAAGGGAAAG+TGG + chr4_4:65975947-65975966 Msa0696130:intron 25.0%
! GAAAGTGGAATATATTGTAT+TGG + chr4_4:65975962-65975981 Msa0696130:intron 25.0%
!! TTGAAATTGGTCAGTTATTT+TGG + chr4_4:65976050-65976069 Msa0696130:intron 25.0%
!!! TGAAATTGGTCAGTTATTTT+GGG + chr4_4:65976051-65976070 Msa0696130:intron 25.0%
!!! GGACACTTTTTTTTAGAAAA+TGG + chr4_4:65976072-65976091 Msa0696130:intron 25.0%
!!! GAAGAAACAGTTTTTTTTGT+TGG + chr4_4:65976279-65976298 Msa0696130:three_prime_UTR 25.0%
GATCTCAAAGATTATGTTAC+TGG + chr4_4:65973996-65974015 Msa0696130:CDS 30.0%
! AATTTTATTGCAGAGGAAGT+TGG + chr4_4:65974178-65974197 Msa0696130:intron 30.0%
AAACAAGTTCAATCTATTGC+TGG + chr4_4:65974287-65974306 Msa0696130:CDS 30.0%
AAACAATTGTGAGACTGATT+GGG - chr4_4:65974554-65974573 None:intergenic 30.0%
TAAACAATTGTGAGACTGAT+TGG - chr4_4:65974555-65974574 None:intergenic 30.0%
ACCTATATGAGCTTAACTAT+TGG - chr4_4:65974580-65974599 None:intergenic 30.0%
TTTACAACTAACACTAAGCA+TGG - chr4_4:65974690-65974709 None:intergenic 30.0%
TGCTTAGTGTTAGTTGTAAA+AGG + chr4_4:65974690-65974709 Msa0696130:intron 30.0%
ATTAAGTTCCTTCTTCATAC+TGG + chr4_4:65974730-65974749 Msa0696130:intron 30.0%
! GAGTTAGCTTAATGTTTACT+AGG + chr4_4:65974799-65974818 Msa0696130:intron 30.0%
GAACTAAAAATTTGTACTGG+AGG + chr4_4:65974998-65975017 Msa0696130:intron 30.0%
TAAAAATTTGTACTGGAGGA+GGG + chr4_4:65975002-65975021 Msa0696130:intron 30.0%
! GTGATTTTTCTTACTGCATT+TGG + chr4_4:65975465-65975484 Msa0696130:intron 30.0%
! TTCTTTTTATCAGCTACAAC+AGG - chr4_4:65975655-65975674 None:intergenic 30.0%
! TTTGTTGCAGCCTTTTTAAA+TGG - chr4_4:65975703-65975722 None:intergenic 30.0%
AGTAAGAGAGAAAAAAGGAA+AGG + chr4_4:65975748-65975767 Msa0696130:CDS 30.0%
!! ATAAAGTTTGTTGCAGACTT+GGG - chr4_4:65975829-65975848 None:intergenic 30.0%
!! AATAAAGTTTGTTGCAGACT+TGG - chr4_4:65975830-65975849 None:intergenic 30.0%
GCAACAAACTTTATTCTTTG+TGG + chr4_4:65975835-65975854 Msa0696130:three_prime_UTR 30.0%
ACAAACTTTATTCTTTGTGG+AGG + chr4_4:65975838-65975857 Msa0696130:three_prime_UTR 30.0%
GTAAGTCAATTAAATTGGGA+CGG - chr4_4:65975899-65975918 None:intergenic 30.0%
AGTAACTTATTGCAGAGTTT+GGG + chr4_4:65976118-65976137 Msa0696130:intron 30.0%
TTCATTAAATATGCGCTAGT+AGG + chr4_4:65976153-65976172 Msa0696130:intron 30.0%
CATTAGAAGATCACATACAA+AGG - chr4_4:65976460-65976479 None:intergenic 30.0%
! AATAGGAGGGTTTTTGTACA+CGG + chr4_4:65973833-65973852 Msa0696130:five_prime_UTR 35.0%
ATCTTTCTCATCGAAAGAGA+TGG - chr4_4:65973979-65973998 None:intergenic 35.0%
!! AGCTTCTTCTTGTTGTTTGT+TGG - chr4_4:65974057-65974076 None:intergenic 35.0%
! TTGGAAAGAGAACTTGCTTA+TGG + chr4_4:65974197-65974216 Msa0696130:CDS 35.0%
! TGGAAAGAGAACTTGCTTAT+GGG + chr4_4:65974198-65974217 Msa0696130:CDS 35.0%
GTTTGTCAGTTTCTTCTTCT+TGG - chr4_4:65974271-65974290 None:intergenic 35.0%
GCTTATTGTATAAACTCTCG+AGG - chr4_4:65974486-65974505 None:intergenic 35.0%
GCCAATAGTTAAGCTCATAT+AGG + chr4_4:65974576-65974595 Msa0696130:intron 35.0%
TTCATCATCAGACACGTTTA+AGG - chr4_4:65974617-65974636 None:intergenic 35.0%
AATGTATGCCAGTATGAAGA+AGG - chr4_4:65974741-65974760 None:intergenic 35.0%
CTTAATGTTTACTAGGACTG+AGG + chr4_4:65974806-65974825 Msa0696130:intron 35.0%
TGGGAGAAATAGAGTACATT+GGG + chr4_4:65974916-65974935 Msa0696130:intron 35.0%
AGAAATAGAGTACATTGGGA+GGG + chr4_4:65974920-65974939 Msa0696130:intron 35.0%
AATACAAAACCGTCCTCATT+TGG + chr4_4:65974973-65974992 Msa0696130:intron 35.0%
ATACAAAACCGTCCTCATTT+GGG + chr4_4:65974974-65974993 Msa0696130:intron 35.0%
!!! ATTTTTAGTTCCCCCAAATG+AGG - chr4_4:65974989-65975008 None:intergenic 35.0%
GGGGAACTAAAAATTTGTAC+TGG + chr4_4:65974995-65975014 Msa0696130:intron 35.0%
CTAAAAATTTGTACTGGAGG+AGG + chr4_4:65975001-65975020 Msa0696130:intron 35.0%
GTGGAAAATATAGAGGGAAA+TGG - chr4_4:65975166-65975185 None:intergenic 35.0%
TTTATCAGCTACAACAGGAT+GGG - chr4_4:65975650-65975669 None:intergenic 35.0%
! TTTTATCAGCTACAACAGGA+TGG - chr4_4:65975651-65975670 None:intergenic 35.0%
TCTCTCTTACTTCTTTGCTT+TGG - chr4_4:65975739-65975758 None:intergenic 35.0%
AAAAAGGAAAGGAAAGAAGC+CGG + chr4_4:65975759-65975778 Msa0696130:CDS 35.0%
AGAAAATGGGTCAGTTATTC+CGG + chr4_4:65976086-65976105 Msa0696130:intron 35.0%
GAGTAACTTATTGCAGAGTT+TGG + chr4_4:65976117-65976136 Msa0696130:intron 35.0%
CAAATGGATCAGCTAAACAA+GGG - chr4_4:65976241-65976260 None:intergenic 35.0%
CTTGTTTAGCTGATCCATTT+GGG + chr4_4:65976240-65976259 Msa0696130:three_prime_UTR 35.0%
TTGTTTAGCTGATCCATTTG+GGG + chr4_4:65976241-65976260 Msa0696130:three_prime_UTR 35.0%
! ATAGGAGGGTTTTTGTACAC+GGG + chr4_4:65973834-65973853 Msa0696130:five_prime_UTR 40.0%
TTCTTCATCCATGGTTTCCA+GGG - chr4_4:65973892-65973911 None:intergenic 40.0%
! TATTGCTGCTTCTTCATCCA+TGG - chr4_4:65973901-65973920 None:intergenic 40.0%
! GGAAAGAGAACTTGCTTATG+GGG + chr4_4:65974199-65974218 Msa0696130:CDS 40.0%
! GAAAGAGAACTTGCTTATGG+GGG + chr4_4:65974200-65974219 Msa0696130:CDS 40.0%
CATCAGACACGTTTAAGGAT+TGG - chr4_4:65974612-65974631 None:intergenic 40.0%
CAAGACATGAGGACTGATTA+AGG - chr4_4:65974774-65974793 None:intergenic 40.0%
ACTCGCAAACAAAGGCTAAT+AGG - chr4_4:65974854-65974873 None:intergenic 40.0%
! TTAGCCTTTGTTTGCGAGTT+TGG + chr4_4:65974855-65974874 Msa0696130:intron 40.0%
!!! GATGGTTTTGGAAAAGAGGA+AGG + chr4_4:65974887-65974906 Msa0696130:intron 40.0%
GAAAAGAGGAAGGAGAAAGT+GGG + chr4_4:65974897-65974916 Msa0696130:intron 40.0%
GTGGGAGAAATAGAGTACAT+TGG + chr4_4:65974915-65974934 Msa0696130:intron 40.0%
GAGAAATAGAGTACATTGGG+AGG + chr4_4:65974919-65974938 Msa0696130:intron 40.0%
GAAATAGAGTACATTGGGAG+GGG + chr4_4:65974921-65974940 Msa0696130:intron 40.0%
TACAAAACCGTCCTCATTTG+GGG + chr4_4:65974975-65974994 Msa0696130:intron 40.0%
!! TAAGGCTTTGTTTGGGAGTT+TGG - chr4_4:65975217-65975236 None:intergenic 40.0%
!! CGACAACTAAGGCTTTGTTT+GGG - chr4_4:65975224-65975243 None:intergenic 40.0%
CCAAACAAAGCCTTAGTTGT+CGG + chr4_4:65975222-65975241 Msa0696130:intron 40.0%
AAAAGGAAAGGAAAGAAGCC+GGG + chr4_4:65975760-65975779 Msa0696130:CDS 40.0%
ACCTTGGCATATTGCTTCTT+CGG + chr4_4:65975797-65975816 Msa0696130:three_prime_UTR 40.0%
AGTCAATTAAATTGGGACGG+AGG - chr4_4:65975896-65975915 None:intergenic 40.0%
GAAAATGGGTCAGTTATTCC+GGG + chr4_4:65976087-65976106 Msa0696130:intron 40.0%
AGGTTACTGAGTTCCTTATG+TGG + chr4_4:65976173-65976192 Msa0696130:intron 40.0%
ACTGAGTTCCTTATGTGGAT+TGG + chr4_4:65976178-65976197 Msa0696130:intron 40.0%
!! AGTTCCTTATGTGGATTGGA+TGG + chr4_4:65976182-65976201 Msa0696130:intron 40.0%
CCAAATGGATCAGCTAAACA+AGG - chr4_4:65976242-65976261 None:intergenic 40.0%
! CCTTGTTTAGCTGATCCATT+TGG + chr4_4:65976239-65976258 Msa0696130:three_prime_UTR 40.0%
TGTTTAGCTGATCCATTTGG+GGG + chr4_4:65976242-65976261 Msa0696130:three_prime_UTR 40.0%
AATTTCAGTGTCTCTCAAGC+TGG + chr4_4:65976395-65976414 Msa0696130:three_prime_UTR 40.0%
TCGTAGGTTGTGGTTGATTG+CGG + chr4_4:65973861-65973880 Msa0696130:five_prime_UTR 45.0%
CTTCTTCATCCATGGTTTCC+AGG - chr4_4:65973893-65973912 None:intergenic 45.0%
GCAATATCAGAGCGCAAAAC+TGG + chr4_4:65973915-65973934 Msa0696130:CDS 45.0%
! TTTAAGACGCAAACGCAACG+AGG + chr4_4:65974076-65974095 Msa0696130:CDS 45.0%
GTTTCAGTGTCATCAGTTGG+TGG - chr4_4:65974230-65974249 None:intergenic 45.0%
CCAGTTTCAGTGTCATCAGT+TGG - chr4_4:65974233-65974252 None:intergenic 45.0%
CCAACTGATGACACTGAAAC+TGG + chr4_4:65974230-65974249 Msa0696130:CDS 45.0%
TAACTCGTGCACAAGACATG+AGG - chr4_4:65974785-65974804 None:intergenic 45.0%
! CCTTTGTTTGCGAGTTTGGA+GGG + chr4_4:65974859-65974878 Msa0696130:intron 45.0%
! CTTTGTTTGCGAGTTTGGAG+GGG + chr4_4:65974860-65974879 Msa0696130:intron 45.0%
!!! AGGGGATGGTTTTGGAAAAG+AGG + chr4_4:65974883-65974902 Msa0696130:intron 45.0%
GGAAAAGAGGAAGGAGAAAG+TGG + chr4_4:65974896-65974915 Msa0696130:intron 45.0%
ACAAAACCGTCCTCATTTGG+GGG + chr4_4:65974976-65974995 Msa0696130:intron 45.0%
TTAGTTCCCCCAAATGAGGA+CGG - chr4_4:65974985-65975004 None:intergenic 45.0%
! TTTGTACTGGAGGAGGGTTT+TGG + chr4_4:65975008-65975027 Msa0696130:intron 45.0%
! TTGTACTGGAGGAGGGTTTT+GGG + chr4_4:65975009-65975028 Msa0696130:intron 45.0%
TGGGGGGTGGAAAATATAGA+GGG - chr4_4:65975172-65975191 None:intergenic 45.0%
TTGGGGGGTGGAAAATATAG+AGG - chr4_4:65975173-65975192 None:intergenic 45.0%
! CTTTGTTTGGGAGTTTGGAG+GGG - chr4_4:65975212-65975231 None:intergenic 45.0%
!! GCTTTGTTTGGGAGTTTGGA+GGG - chr4_4:65975213-65975232 None:intergenic 45.0%
!! CCGACAACTAAGGCTTTGTT+TGG - chr4_4:65975225-65975244 None:intergenic 45.0%
TAGGCAACTACCGACAACTA+AGG - chr4_4:65975235-65975254 None:intergenic 45.0%
TAGTGCGTAGCATAGAGTGA+TGG - chr4_4:65975342-65975361 None:intergenic 45.0%
GTGTAGTGTAGAGGGCTATT+TGG + chr4_4:65975421-65975440 Msa0696130:intron 45.0%
TAGGGAAACTCTCTTCGTCA+GGG - chr4_4:65975594-65975613 None:intergenic 45.0%
AAGAGAGTTTCCCTAGCAAG+AGG + chr4_4:65975599-65975618 Msa0696130:CDS 45.0%
TCCGAAGAAGCAATATGCCA+AGG - chr4_4:65975801-65975820 None:intergenic 45.0%
! ATGGCAGGTTTCTCTTTTGC+TGG + chr4_4:65976201-65976220 Msa0696130:intron 45.0%
GTTTAGCTGATCCATTTGGG+GGG + chr4_4:65976243-65976262 Msa0696130:three_prime_UTR 45.0%
!! GATTCTGAAATCCCCCCAAA+TGG - chr4_4:65976257-65976276 None:intergenic 45.0%
TTTGTACACGGGTGAGTCGT+AGG + chr4_4:65973845-65973864 Msa0696130:five_prime_UTR 50.0%
ACGCAACGAGGAACGCATAA+AGG + chr4_4:65974088-65974107 Msa0696130:CDS 50.0%
! GCCTTTGTTTGCGAGTTTGG+AGG + chr4_4:65974858-65974877 Msa0696130:intron 50.0%
CCCTCCAAACTCGCAAACAA+AGG - chr4_4:65974862-65974881 None:intergenic 50.0%
ATAGAGTACATTGGGAGGGG+AGG + chr4_4:65974924-65974943 Msa0696130:intron 50.0%
TAGAGTACATTGGGAGGGGA+GGG + chr4_4:65974925-65974944 Msa0696130:intron 50.0%
! TGTACTGGAGGAGGGTTTTG+GGG + chr4_4:65975010-65975029 Msa0696130:intron 50.0%
!! GGCTTTGTTTGGGAGTTTGG+AGG - chr4_4:65975214-65975233 None:intergenic 50.0%
GTTGCCTATAGCACTAGCGT+TGG + chr4_4:65975247-65975266 Msa0696130:intron 50.0%
TGCGTAGCATAGAGTGATGG+CGG - chr4_4:65975339-65975358 None:intergenic 50.0%
TGCTACGCACTATCCCACTT+TGG + chr4_4:65975350-65975369 Msa0696130:intron 50.0%
GCTACGCACTATCCCACTTT+GGG + chr4_4:65975351-65975370 Msa0696130:intron 50.0%
! CACTATCCCACTTTGGGAGT+CGG + chr4_4:65975357-65975376 Msa0696130:intron 50.0%
! GAAAAGCCGACTCCCAAAGT+GGG - chr4_4:65975366-65975385 None:intergenic 50.0%
GTCAATCCTGGATAGCAGAG+CGG - chr4_4:65975388-65975407 None:intergenic 50.0%
TACGGCATAGTGGTCAATCC+TGG - chr4_4:65975400-65975419 None:intergenic 50.0%
ACACTACACGTACGGCATAG+TGG - chr4_4:65975410-65975429 None:intergenic 50.0%
ATGCCGTACGTGTAGTGTAG+AGG + chr4_4:65975412-65975431 Msa0696130:intron 50.0%
TGCCGTACGTGTAGTGTAGA+GGG + chr4_4:65975413-65975432 Msa0696130:intron 50.0%
AGCCCTCTACACTACACGTA+CGG - chr4_4:65975418-65975437 None:intergenic 50.0%
CTAGGGAAACTCTCTTCGTC+AGG - chr4_4:65975595-65975614 None:intergenic 50.0%
GGGCTTCTTTCCTCTTGCTA+GGG - chr4_4:65975612-65975631 None:intergenic 50.0%
ATCAGCTACAACAGGATGGG+AGG - chr4_4:65975647-65975666 None:intergenic 50.0%
AGAAGCCGGGAAACAAGTGA+CGG + chr4_4:65975773-65975792 Msa0696130:CDS 50.0%
! AGGTACCGTCACTTGTTTCC+CGG - chr4_4:65975781-65975800 None:intergenic 50.0%
GGAAACAAGTGACGGTACCT+TGG + chr4_4:65975781-65975800 Msa0696130:exon 50.0%
ATGGGTCAGTTATTCCGGGA+CGG + chr4_4:65976091-65976110 Msa0696130:intron 50.0%
ATAAGTTACTCCCTCCGTCC+CGG - chr4_4:65976108-65976127 None:intergenic 50.0%
CCTGCCATCCAATCCACATA+AGG - chr4_4:65976189-65976208 None:intergenic 50.0%
!! CCTTATGTGGATTGGATGGC+AGG + chr4_4:65976186-65976205 Msa0696130:intron 50.0%
TGTTTGCGAGTTTGGAGGGG+AGG + chr4_4:65974863-65974882 Msa0696130:intron 55.0%
GTTTGCGAGTTTGGAGGGGA+GGG + chr4_4:65974864-65974883 Msa0696130:intron 55.0%
TTTGCGAGTTTGGAGGGGAG+GGG + chr4_4:65974865-65974884 Msa0696130:intron 55.0%
!! GTACTGGAGGAGGGTTTTGG+GGG + chr4_4:65975011-65975030 Msa0696130:intron 55.0%
!!! TACTGGAGGAGGGTTTTGGG+GGG + chr4_4:65975012-65975031 Msa0696130:intron 55.0%
! AGGGGTAGGAAAGGCTTTGG+GGG - chr4_4:65975189-65975208 None:intergenic 55.0%
GAGGGGTAGGAAAGGCTTTG+GGG - chr4_4:65975190-65975209 None:intergenic 55.0%
GGAGGGGTAGGAAAGGCTTT+GGG - chr4_4:65975191-65975210 None:intergenic 55.0%
TTTGGGAGTTTGGAGGGGAG+GGG - chr4_4:65975207-65975226 None:intergenic 55.0%
GTTTGGGAGTTTGGAGGGGA+GGG - chr4_4:65975208-65975227 None:intergenic 55.0%
TGTTTGGGAGTTTGGAGGGG+AGG - chr4_4:65975209-65975228 None:intergenic 55.0%
GGCTCCAACGCTAGTGCTAT+AGG - chr4_4:65975254-65975273 None:intergenic 55.0%
! GGAAAAGCCGACTCCCAAAG+TGG - chr4_4:65975367-65975386 None:intergenic 55.0%
! GCTTTTCCGCTCTGCTATCC+AGG + chr4_4:65975379-65975398 Msa0696130:intron 55.0%
GGGGCTTCTTTCCTCTTGCT+AGG - chr4_4:65975613-65975632 None:intergenic 55.0%
GTCAGTTATTCCGGGACGGA+GGG + chr4_4:65976095-65976114 Msa0696130:intron 55.0%
CACGGGTGAGTCGTAGGTTG+TGG + chr4_4:65973851-65973870 Msa0696130:five_prime_UTR 60.0%
GGTTGATTGCGGCTGAACCC+TGG + chr4_4:65973872-65973891 Msa0696130:five_prime_UTR 60.0%
CGGCTGAACCCTGGAAACCA+TGG + chr4_4:65973881-65973900 Msa0696130:exon 60.0%
! TGGAGGGGAGGGGATGGTTT+TGG + chr4_4:65974875-65974894 Msa0696130:intron 60.0%
ACATTGGGAGGGGAGGGCTT+TGG + chr4_4:65974931-65974950 Msa0696130:intron 60.0%
CATTGGGAGGGGAGGGCTTT+GGG + chr4_4:65974932-65974951 Msa0696130:intron 60.0%
ATTGGGAGGGGAGGGCTTTG+GGG + chr4_4:65974933-65974952 Msa0696130:intron 60.0%
!! GTAGGAAAGGCTTTGGGGGG+TGG - chr4_4:65975185-65975204 None:intergenic 60.0%
!! GGGGTAGGAAAGGCTTTGGG+GGG - chr4_4:65975188-65975207 None:intergenic 60.0%
GGGAGGGGTAGGAAAGGCTT+TGG - chr4_4:65975192-65975211 None:intergenic 60.0%
TTGGAGGGGAGGGGTAGGAA+AGG - chr4_4:65975198-65975217 None:intergenic 60.0%
! GCACTAGCGTTGGAGCCTAG+CGG + chr4_4:65975257-65975276 Msa0696130:intron 60.0%
GAGCCTAGCGGAGTTGAGTG+TGG + chr4_4:65975269-65975288 Msa0696130:intron 60.0%
AGGCCACACTCAACTCCGCT+AGG - chr4_4:65975275-65975294 None:intergenic 60.0%
TCCCGCGTTCCAACGCATCT+GGG - chr4_4:65975300-65975319 None:intergenic 60.0%
!! AGAGTGATGGCGGCCACGAA+GGG - chr4_4:65975329-65975348 None:intergenic 60.0%
!! TAGAGTGATGGCGGCCACGA+AGG - chr4_4:65975330-65975349 None:intergenic 60.0%
ATGGGAGGACACTGCTGGTG+GGG - chr4_4:65975632-65975651 None:intergenic 60.0%
GATGGGAGGACACTGCTGGT+GGG - chr4_4:65975633-65975652 None:intergenic 60.0%
ACAGGATGGGAGGACACTGC+TGG - chr4_4:65975637-65975656 None:intergenic 60.0%
GGTCAGTTATTCCGGGACGG+AGG + chr4_4:65976094-65976113 Msa0696130:intron 60.0%
CGAGTTTGGAGGGGAGGGGA+TGG + chr4_4:65974869-65974888 Msa0696130:intron 65.0%
! TTGGGAGGGGAGGGCTTTGG+GGG + chr4_4:65974934-65974953 Msa0696130:intron 65.0%
GGAGTTTGGAGGGGAGGGGT+AGG - chr4_4:65975203-65975222 None:intergenic 65.0%
CGTTCCAACGCATCTGGGGG+AGG - chr4_4:65975295-65975314 None:intergenic 65.0%
CCGCGTTCCAACGCATCTGG+GGG - chr4_4:65975298-65975317 None:intergenic 65.0%
CCCCCAGATGCGTTGGAACG+CGG + chr4_4:65975295-65975314 Msa0696130:intron 65.0%
CCCGCGTTCCAACGCATCTG+GGG - chr4_4:65975299-65975318 None:intergenic 65.0%
CCCCAGATGCGTTGGAACGC+GGG + chr4_4:65975296-65975315 Msa0696130:intron 65.0%
CTCCCGCGTTCCAACGCATC+TGG - chr4_4:65975301-65975320 None:intergenic 65.0%
GGATGGGAGGACACTGCTGG+TGG - chr4_4:65975634-65975653 None:intergenic 65.0%
GTGGCCTCCCCCAGATGCGT+TGG + chr4_4:65975288-65975307 Msa0696130:intron 70.0%
CGCGGGAGTAGCGCCCTTCG+TGG + chr4_4:65975313-65975332 Msa0696130:intron 75.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 65973813 65976498 65973813 ID=Msa0696130;Name=Msa0696130
chr4_4 mRNA 65973813 65976498 65973813 ID=Msa0696130-mRNA-1;Parent=Msa0696130;Name=Msa0696130-mRNA-1;_AED=0.12;_eAED=0.12;_QI=87|1|1|1|0.66|0.5|4|358|195
chr4_4 exon 65973813 65974109 65973813 ID=Msa0696130-mRNA-1:exon:19105;Parent=Msa0696130-mRNA-1
chr4_4 exon 65974191 65974308 65974191 ID=Msa0696130-mRNA-1:exon:19106;Parent=Msa0696130-mRNA-1
chr4_4 exon 65975533 65975859 65975533 ID=Msa0696130-mRNA-1:exon:19107;Parent=Msa0696130-mRNA-1
chr4_4 exon 65976208 65976498 65976208 ID=Msa0696130-mRNA-1:exon:19108;Parent=Msa0696130-mRNA-1
chr4_4 five_prime_UTR 65973813 65973899 65973813 ID=Msa0696130-mRNA-1:five_prime_utr;Parent=Msa0696130-mRNA-1
chr4_4 CDS 65973900 65974109 65973900 ID=Msa0696130-mRNA-1:cds;Parent=Msa0696130-mRNA-1
chr4_4 CDS 65974191 65974308 65974191 ID=Msa0696130-mRNA-1:cds;Parent=Msa0696130-mRNA-1
chr4_4 CDS 65975533 65975792 65975533 ID=Msa0696130-mRNA-1:cds;Parent=Msa0696130-mRNA-1
chr4_4 three_prime_UTR 65975793 65975859 65975793 ID=Msa0696130-mRNA-1:three_prime_utr;Parent=Msa0696130-mRNA-1
chr4_4 three_prime_UTR 65976208 65976498 65976208 ID=Msa0696130-mRNA-1:three_prime_utr;Parent=Msa0696130-mRNA-1
Gene Sequence

>Msa0696130

ATGGATGAAGAAGCAGCAATATCAGAGCGCAAAACTGGTCACGTTAAGAAGAGAGCTTTAAAGAACAAAGCTTTAGCCATCTCTTTCGATGAGAAAGATCTCAAAGATTATGTTACTGGCTTTCATAAACGCAAGAAGAAGAGAAGAAAAGAAGCCAACAAACAACAAGAAGAAGCTTTAAGACGCAAACGCAACGAGGAACGCATAAAGAGGAAGTTGGAAAGAGAACTTGCTTATGGGGGTGTGCCACCAACTGATGACACTGAAACTGGTGAAATTGATGACAACCAAGAAGAAGAAACTGACAAACAAGTTCAATCTATTGCTGCCACAAAGACATATGAAAATGATGATTTGAAAGTCACTGTTGTAACAAAAGAGATCAACCCTGACGAAGAGAGTTTCCCTAGCAAGAGGAAAGAAGCCCCACCAGCAGTGTCCTCCCATCCTGTTGTAGCTGATAAAAAGAAATCTATTCCTATAAATAACAAGAAGCCATTTAAAAAGGCTGCAACAAAGCACAAGTCACAGCCAAAGCAAAGAAGTAAGAGAGAAAAAAGGAAAGGAAAGAAGCCGGGAAACAAGTGA
Protein sequence

>Msa0696130

MDEEAAISERKTGHVKKRALKNKALAISFDEKDLKDYVTGFHKRKKKRRKEANKQQEEALRRKRNEERIKRKLERELAYGGVPPTDDTETGEIDDNQEEETDKQVQSIAATKTYENDDLKVTVVTKEINPDEESFPSKRKEAPPAVSSHPVVADKKKSIPINNKKPFKKAATKHKSQPKQRSKREKRKGKKPGNK*