AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1360140


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1360140 MtrunA17_Chr2g0279161 95.333 150 5 2 1 149 183 331 7.79e-94 274
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1360140 AT2G37990.1 66.129 124 39 2 1 122 182 304 2.75e-46 153

Find 31 sgRNAs with CRISPR-Local

Find 126 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATCAAAATTACTGTGCTTT+TGG 0.169446 tig0016946:-672 Msa1360140:intergenic
CGATTCTTTCCTTTATGTTT+TGG 0.214740 tig0016946:-184 Msa1360140:intergenic
CCTTAAATGACAATATAAAA+TGG 0.282907 tig0016946:-778 Msa1360140:intergenic
GAGTATATCATGATTATTCT+TGG 0.294298 tig0016946:-408 Msa1360140:intergenic
GGTGAGTGGGTTCCTGTTAT+AGG 0.329789 tig0016946:-55 Msa1360140:intergenic
TGATTATACTGGAAATTAAC+AGG 0.406511 tig0016946:+829 Msa1360140:three_prime_UTR
CTGCCGGTAGCAGAAGGAAC+AGG 0.421319 tig0016946:+332 Msa1360140:CDS
GGCACCACAAGTGATTATAC+TGG 0.475390 tig0016946:+818 Msa1360140:three_prime_UTR
ACCCCTGTTCCTTCTGCTAC+CGG 0.485546 tig0016946:-335 Msa1360140:intergenic
AGGAACAGGGGTAGGATCAA+AGG 0.493785 tig0016946:+346 Msa1360140:CDS
CACAGTGACCAATGTGAAGA+AGG 0.507110 tig0016946:+551 Msa1360140:CDS
TACACTGCCTAATTCATCCT+TGG 0.511559 tig0016946:-92 Msa1360140:intergenic
GTATAATCACTTGTGGTGCC+TGG 0.511970 tig0016946:-815 Msa1360140:intergenic
TGTGGTGCCTGGTGTTGTGA+TGG 0.514224 tig0016946:-804 Msa1360140:intergenic
ACCACTAGCTGTGGCAGTTG+AGG 0.531694 tig0016946:-125 Msa1360140:intergenic
TGAAGAAGGAGAAAAGCAGA+AGG 0.542891 tig0016946:+565 Msa1360140:CDS
TAGTTCCTGCCGGTAGCAGA+AGG 0.558864 tig0016946:+326 Msa1360140:intron
ATTTCCAGTATAATCACTTG+TGG 0.569183 tig0016946:-822 Msa1360140:intergenic
ATGTCGCGGCCAACTGAACA+TGG 0.570065 tig0016946:-27 Msa1360140:intergenic
CTGTTCCTTCTGCTACCGGC+AGG 0.578497 tig0016946:-331 Msa1360140:intergenic
AAGGCTTCCATCACAACACC+AGG 0.597161 tig0016946:+797 Msa1360140:three_prime_UTR
GCCAACTGAACATGGCTGCA+TGG 0.604164 tig0016946:-19 Msa1360140:intergenic
TGCCGGTAGCAGAAGGAACA+GGG 0.605130 tig0016946:+333 Msa1360140:CDS
CAGAAGGAATGATAAATCGA+AGG 0.608042 tig0016946:+581 Msa1360140:CDS
AGGGGTAGGATCAAAGGAGA+GGG 0.618168 tig0016946:+352 Msa1360140:CDS
GTAGCAGAAGGAACAGGGGT+AGG 0.632666 tig0016946:+338 Msa1360140:CDS
AAACTTCCCACCACTAGCTG+TGG 0.634673 tig0016946:-134 Msa1360140:intergenic
TGATATACTCAATGTTAACA+AGG 0.643030 tig0016946:+421 Msa1360140:CDS
CAGGGGTAGGATCAAAGGAG+AGG 0.644223 tig0016946:+351 Msa1360140:CDS
TGTTATAGGCAAAGATGTCG+CGG 0.661140 tig0016946:-41 Msa1360140:intergenic
GCCGGTAGCAGAAGGAACAG+GGG 0.714140 tig0016946:+334 Msa1360140:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CACAGTAATTTTGATTTTTA+TGG + tig0016946:679-698 Msa1360140:exon 20.0%
!! CCTTAAATGACAATATAAAA+TGG - tig0016946:781-800 Msa1360140:intergenic 20.0%
!!! CCATTTTATATTGTCATTTA+AGG + tig0016946:778-797 Msa1360140:three_prime_UTR 20.0%
!!! CACAGTAATTTTGATTTTTA+TGG + tig0016946:679-698 Msa1360140:exon 20.0%
!! CCTTAAATGACAATATAAAA+TGG - tig0016946:781-800 Msa1360140:intergenic 20.0%
!!! CCATTTTATATTGTCATTTA+AGG + tig0016946:778-797 Msa1360140:three_prime_UTR 20.0%
! TCGACAAATAATTGTTTACA+AGG - tig0016946:252-271 Msa1360140:intergenic 25.0%
! ACTAGATGAAAAGATAACAA+AGG - tig0016946:310-329 Msa1360140:intergenic 25.0%
! GAGTATATCATGATTATTCT+TGG - tig0016946:411-430 Msa1360140:intergenic 25.0%
! TGATATACTCAATGTTAACA+AGG + tig0016946:421-440 Msa1360140:CDS 25.0%
!! ATCAAAATTACTGTGCTTTT+GGG - tig0016946:674-693 Msa1360140:intergenic 25.0%
!! AATCAAAATTACTGTGCTTT+TGG - tig0016946:675-694 Msa1360140:intergenic 25.0%
!!! TATGGAATCTTGTAGTTTTT+TGG + tig0016946:697-716 Msa1360140:three_prime_UTR 25.0%
! TGATTATACTGGAAATTAAC+AGG + tig0016946:829-848 Msa1360140:three_prime_UTR 25.0%
! TCGACAAATAATTGTTTACA+AGG - tig0016946:252-271 Msa1360140:intergenic 25.0%
! ACTAGATGAAAAGATAACAA+AGG - tig0016946:310-329 Msa1360140:intergenic 25.0%
! GAGTATATCATGATTATTCT+TGG - tig0016946:411-430 Msa1360140:intergenic 25.0%
! TGATATACTCAATGTTAACA+AGG + tig0016946:421-440 Msa1360140:CDS 25.0%
!! ATCAAAATTACTGTGCTTTT+GGG - tig0016946:674-693 Msa1360140:intergenic 25.0%
!! AATCAAAATTACTGTGCTTT+TGG - tig0016946:675-694 Msa1360140:intergenic 25.0%
!!! TATGGAATCTTGTAGTTTTT+TGG + tig0016946:697-716 Msa1360140:three_prime_UTR 25.0%
! TGATTATACTGGAAATTAAC+AGG + tig0016946:829-848 Msa1360140:three_prime_UTR 25.0%
AAGTTTGACAAAAAATTGCC+AGG + tig0016946:151-170 Msa1360140:CDS 30.0%
!! TTCTTTCCTTTATGTTTTGG+AGG - tig0016946:184-203 Msa1360140:intergenic 30.0%
! CGATTCTTTCCTTTATGTTT+TGG - tig0016946:187-206 Msa1360140:intergenic 30.0%
ACATAAAGGAAAGAATCGAA+AGG + tig0016946:189-208 Msa1360140:CDS 30.0%
!! TCAGAAGAAGCCTTTTAAAA+AGG + tig0016946:632-651 Msa1360140:CDS 30.0%
!! CAGAAGAAGCCTTTTAAAAA+GGG + tig0016946:633-652 Msa1360140:CDS 30.0%
! TTTTTGGATACACTTTTCAG+TGG + tig0016946:713-732 Msa1360140:three_prime_UTR 30.0%
ATTTCCAGTATAATCACTTG+TGG - tig0016946:825-844 Msa1360140:intergenic 30.0%
AAGTTTGACAAAAAATTGCC+AGG + tig0016946:151-170 Msa1360140:CDS 30.0%
!! TTCTTTCCTTTATGTTTTGG+AGG - tig0016946:184-203 Msa1360140:intergenic 30.0%
! CGATTCTTTCCTTTATGTTT+TGG - tig0016946:187-206 Msa1360140:intergenic 30.0%
ACATAAAGGAAAGAATCGAA+AGG + tig0016946:189-208 Msa1360140:CDS 30.0%
!! TCAGAAGAAGCCTTTTAAAA+AGG + tig0016946:632-651 Msa1360140:CDS 30.0%
!! CAGAAGAAGCCTTTTAAAAA+GGG + tig0016946:633-652 Msa1360140:CDS 30.0%
! TTTTTGGATACACTTTTCAG+TGG + tig0016946:713-732 Msa1360140:three_prime_UTR 30.0%
ATTTCCAGTATAATCACTTG+TGG - tig0016946:825-844 Msa1360140:intergenic 30.0%
AAGGTTACCAAGGATGAATT+AGG + tig0016946:85-104 Msa1360140:CDS 35.0%
GAAAAGCCTCCAAAACATAA+AGG + tig0016946:175-194 Msa1360140:CDS 35.0%
AATTGTACTCTCTGCATTCT+AGG + tig0016946:524-543 Msa1360140:intron 35.0%
CAGAAGGAATGATAAATCGA+AGG + tig0016946:581-600 Msa1360140:CDS 35.0%
! TTCTTGGCACCCTTTTTAAA+AGG - tig0016946:645-664 Msa1360140:intergenic 35.0%
!! TTTAAAAAGGGTGCCAAGAA+AGG + tig0016946:645-664 Msa1360140:CDS 35.0%
AAGGTTACCAAGGATGAATT+AGG + tig0016946:85-104 Msa1360140:CDS 35.0%
GAAAAGCCTCCAAAACATAA+AGG + tig0016946:175-194 Msa1360140:CDS 35.0%
AATTGTACTCTCTGCATTCT+AGG + tig0016946:524-543 Msa1360140:intron 35.0%
CAGAAGGAATGATAAATCGA+AGG + tig0016946:581-600 Msa1360140:CDS 35.0%
! TTCTTGGCACCCTTTTTAAA+AGG - tig0016946:645-664 Msa1360140:intergenic 35.0%
!! TTTAAAAAGGGTGCCAAGAA+AGG + tig0016946:645-664 Msa1360140:CDS 35.0%
TGTTATAGGCAAAGATGTCG+CGG - tig0016946:44-63 Msa1360140:intergenic 40.0%
! AACCTTCTTTTGTGGTGAGT+GGG - tig0016946:71-90 Msa1360140:intergenic 40.0%
! TAACCTTCTTTTGTGGTGAG+TGG - tig0016946:72-91 Msa1360140:intergenic 40.0%
ACCACAAAAGAAGGTTACCA+AGG + tig0016946:75-94 Msa1360140:CDS 40.0%
!! TCCTTGGTAACCTTCTTTTG+TGG - tig0016946:79-98 Msa1360140:intergenic 40.0%
TACACTGCCTAATTCATCCT+TGG - tig0016946:95-114 Msa1360140:intergenic 40.0%
!! TCTTTTCATCTAGTTCCTGC+CGG + tig0016946:316-335 Msa1360140:intron 40.0%
! GCTTTTCTCCTTCTTCACAT+TGG - tig0016946:562-581 Msa1360140:intergenic 40.0%
TGAAGAAGGAGAAAAGCAGA+AGG + tig0016946:565-584 Msa1360140:CDS 40.0%
TGTTATAGGCAAAGATGTCG+CGG - tig0016946:44-63 Msa1360140:intergenic 40.0%
! AACCTTCTTTTGTGGTGAGT+GGG - tig0016946:71-90 Msa1360140:intergenic 40.0%
! TAACCTTCTTTTGTGGTGAG+TGG - tig0016946:72-91 Msa1360140:intergenic 40.0%
ACCACAAAAGAAGGTTACCA+AGG + tig0016946:75-94 Msa1360140:CDS 40.0%
!! TCCTTGGTAACCTTCTTTTG+TGG - tig0016946:79-98 Msa1360140:intergenic 40.0%
TACACTGCCTAATTCATCCT+TGG - tig0016946:95-114 Msa1360140:intergenic 40.0%
!! TCTTTTCATCTAGTTCCTGC+CGG + tig0016946:316-335 Msa1360140:intron 40.0%
! GCTTTTCTCCTTCTTCACAT+TGG - tig0016946:562-581 Msa1360140:intergenic 40.0%
TGAAGAAGGAGAAAAGCAGA+AGG + tig0016946:565-584 Msa1360140:CDS 40.0%
GCGACATCTTTGCCTATAAC+AGG + tig0016946:43-62 Msa1360140:CDS 45.0%
AACCCACTCACCACAAAAGA+AGG + tig0016946:66-85 Msa1360140:CDS 45.0%
GATGAATTAGGCAGTGTAGC+AGG + tig0016946:97-116 Msa1360140:CDS 45.0%
CACAGTGACCAATGTGAAGA+AGG + tig0016946:551-570 Msa1360140:CDS 45.0%
!!! TGCTTTTGGGTTGCCTTTCT+TGG - tig0016946:661-680 Msa1360140:intergenic 45.0%
GTATAATCACTTGTGGTGCC+TGG - tig0016946:818-837 Msa1360140:intergenic 45.0%
GGCACCACAAGTGATTATAC+TGG + tig0016946:818-837 Msa1360140:three_prime_UTR 45.0%
GCGACATCTTTGCCTATAAC+AGG + tig0016946:43-62 Msa1360140:CDS 45.0%
AACCCACTCACCACAAAAGA+AGG + tig0016946:66-85 Msa1360140:CDS 45.0%
GATGAATTAGGCAGTGTAGC+AGG + tig0016946:97-116 Msa1360140:CDS 45.0%
CACAGTGACCAATGTGAAGA+AGG + tig0016946:551-570 Msa1360140:CDS 45.0%
!!! TGCTTTTGGGTTGCCTTTCT+TGG - tig0016946:661-680 Msa1360140:intergenic 45.0%
GTATAATCACTTGTGGTGCC+TGG - tig0016946:818-837 Msa1360140:intergenic 45.0%
GGCACCACAAGTGATTATAC+TGG + tig0016946:818-837 Msa1360140:three_prime_UTR 45.0%
ACCATGCAGCCATGTTCAGT+TGG + tig0016946:18-37 Msa1360140:CDS 50.0%
! GGTGAGTGGGTTCCTGTTAT+AGG - tig0016946:58-77 Msa1360140:intergenic 50.0%
AAACTTCCCACCACTAGCTG+TGG - tig0016946:137-156 Msa1360140:intergenic 50.0%
!!! TGTTTTGGAGGCTTTTCGCC+TGG - tig0016946:172-191 Msa1360140:intergenic 50.0%
AGGAACAGGGGTAGGATCAA+AGG + tig0016946:346-365 Msa1360140:CDS 50.0%
AGGGGTAGGATCAAAGGAGA+GGG + tig0016946:352-371 Msa1360140:CDS 50.0%
AAGGCTTCCATCACAACACC+AGG + tig0016946:797-816 Msa1360140:three_prime_UTR 50.0%
ACCATGCAGCCATGTTCAGT+TGG + tig0016946:18-37 Msa1360140:CDS 50.0%
! GGTGAGTGGGTTCCTGTTAT+AGG - tig0016946:58-77 Msa1360140:intergenic 50.0%
AAACTTCCCACCACTAGCTG+TGG - tig0016946:137-156 Msa1360140:intergenic 50.0%
!!! TGTTTTGGAGGCTTTTCGCC+TGG - tig0016946:172-191 Msa1360140:intergenic 50.0%
AGGAACAGGGGTAGGATCAA+AGG + tig0016946:346-365 Msa1360140:CDS 50.0%
AGGGGTAGGATCAAAGGAGA+GGG + tig0016946:352-371 Msa1360140:CDS 50.0%
AAGGCTTCCATCACAACACC+AGG + tig0016946:797-816 Msa1360140:three_prime_UTR 50.0%
GCCAACTGAACATGGCTGCA+TGG - tig0016946:22-41 Msa1360140:intergenic 55.0%
ATGTCGCGGCCAACTGAACA+TGG - tig0016946:30-49 Msa1360140:intergenic 55.0%
ACCACTAGCTGTGGCAGTTG+AGG - tig0016946:128-147 Msa1360140:intergenic 55.0%
TCAACTGCCACAGCTAGTGG+TGG + tig0016946:127-146 Msa1360140:CDS 55.0%
CAACTGCCACAGCTAGTGGT+GGG + tig0016946:128-147 Msa1360140:CDS 55.0%
TAGTTCCTGCCGGTAGCAGA+AGG + tig0016946:326-345 Msa1360140:intron 55.0%
TGCCGGTAGCAGAAGGAACA+GGG + tig0016946:333-352 Msa1360140:CDS 55.0%
ACCCCTGTTCCTTCTGCTAC+CGG - tig0016946:338-357 Msa1360140:intergenic 55.0%
GTAGCAGAAGGAACAGGGGT+AGG + tig0016946:338-357 Msa1360140:CDS 55.0%
CAGGGGTAGGATCAAAGGAG+AGG + tig0016946:351-370 Msa1360140:CDS 55.0%
!! TGTGGTGCCTGGTGTTGTGA+TGG - tig0016946:807-826 Msa1360140:intergenic 55.0%
GCCAACTGAACATGGCTGCA+TGG - tig0016946:22-41 Msa1360140:intergenic 55.0%
ATGTCGCGGCCAACTGAACA+TGG - tig0016946:30-49 Msa1360140:intergenic 55.0%
ACCACTAGCTGTGGCAGTTG+AGG - tig0016946:128-147 Msa1360140:intergenic 55.0%
TCAACTGCCACAGCTAGTGG+TGG + tig0016946:127-146 Msa1360140:CDS 55.0%
CAACTGCCACAGCTAGTGGT+GGG + tig0016946:128-147 Msa1360140:CDS 55.0%
TAGTTCCTGCCGGTAGCAGA+AGG + tig0016946:326-345 Msa1360140:intron 55.0%
TGCCGGTAGCAGAAGGAACA+GGG + tig0016946:333-352 Msa1360140:CDS 55.0%
ACCCCTGTTCCTTCTGCTAC+CGG - tig0016946:338-357 Msa1360140:intergenic 55.0%
GTAGCAGAAGGAACAGGGGT+AGG + tig0016946:338-357 Msa1360140:CDS 55.0%
CAGGGGTAGGATCAAAGGAG+AGG + tig0016946:351-370 Msa1360140:CDS 55.0%
!! TGTGGTGCCTGGTGTTGTGA+TGG - tig0016946:807-826 Msa1360140:intergenic 55.0%
GCCTCAACTGCCACAGCTAG+TGG + tig0016946:124-143 Msa1360140:CDS 60.0%
CTGTTCCTTCTGCTACCGGC+AGG - tig0016946:334-353 Msa1360140:intergenic 60.0%
CTGCCGGTAGCAGAAGGAAC+AGG + tig0016946:332-351 Msa1360140:CDS 60.0%
GCCGGTAGCAGAAGGAACAG+GGG + tig0016946:334-353 Msa1360140:CDS 60.0%
GCCTCAACTGCCACAGCTAG+TGG + tig0016946:124-143 Msa1360140:CDS 60.0%
CTGTTCCTTCTGCTACCGGC+AGG - tig0016946:334-353 Msa1360140:intergenic 60.0%
CTGCCGGTAGCAGAAGGAAC+AGG + tig0016946:332-351 Msa1360140:CDS 60.0%
GCCGGTAGCAGAAGGAACAG+GGG + tig0016946:334-353 Msa1360140:CDS 60.0%
Chromosome Type Strat End Strand Name
tig0016946 gene 16 888 16 ID=Msa1360140;Name=Msa1360140
tig0016946 mRNA 16 888 16 ID=Msa1360140-mRNA-1;Parent=Msa1360140;Name=Msa1360140-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|1|0.66|1|1|1|3|202|149
tig0016946 exon 16 210 16 ID=Msa1360140-mRNA-1:exon:11788;Parent=Msa1360140-mRNA-1
tig0016946 exon 329 442 329 ID=Msa1360140-mRNA-1:exon:11789;Parent=Msa1360140-mRNA-1
tig0016946 exon 546 888 546 ID=Msa1360140-mRNA-1:exon:11790;Parent=Msa1360140-mRNA-1
tig0016946 CDS 16 210 16 ID=Msa1360140-mRNA-1:cds;Parent=Msa1360140-mRNA-1
tig0016946 CDS 329 442 329 ID=Msa1360140-mRNA-1:cds;Parent=Msa1360140-mRNA-1
tig0016946 CDS 546 686 546 ID=Msa1360140-mRNA-1:cds;Parent=Msa1360140-mRNA-1
tig0016946 three_prime_UTR 687 888 687 ID=Msa1360140-mRNA-1:three_prime_utr;Parent=Msa1360140-mRNA-1
Gene Sequence

>Msa1360140

TTACCATGCAGCCATGTTCAGTTGGCCGCGACATCTTTGCCTATAACAGGAACCCACTCACCACAAAAGAAGGTTACCAAGGATGAATTAGGCAGTGTAGCAGGTTTTGCCTCAACTGCCACAGCTAGTGGTGGGAAGTTTGACAAAAAATTGCCAGGCGAAAAGCCTCCAAAACATAAAGGAAAGAATCGAAAGTTCCTGCCGGTAGCAGAAGGAACAGGGGTAGGATCAAAGGAGAGGGAGCAAACTGAAAATATATTGAACAAGATCATGTCCAAGAATAATCATGATATACTCAATGTTAACAAGGCTGTCACAGTGACCAATGTGAAGAAGGAGAAAAGCAGAAGGAATGATAAATCGAAGGCTTCAGCAACTAACAGCAAATTGAAAACTCAGAAGAAGCCTTTTAAAAAGGGTGCCAAGAAAGGCAACCCAAAAGCACAGTAA

Protein sequence

>Msa1360140

LPCSHVQLAATSLPITGTHSPQKKVTKDELGSVAGFASTATASGGKFDKKLPGEKPPKHKGKNRKFLPVAEGTGVGSKEREQTENILNKIMSKNNHDILNVNKAVTVTNVKKEKSRRNDKSKASATNSKLKTQKKPFKKGAKKGNPKAQ*