Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1007930 | XP_003621337.2 | 67.397 | 365 | 57 | 6 | 456 | 761 | 31 | 392 | 3.35e-153 | 462 |
| Msa1007930 | XP_003621337.2 | 83.333 | 354 | 33 | 4 | 1 | 329 | 25 | 377 | 0.0 | 585 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1007930 | sp|P93324|CHOMT_MEDSA | 59.026 | 349 | 117 | 4 | 7 | 330 | 8 | 355 | 1.12e-135 | 408 |
| Msa1007930 | sp|P93324|CHOMT_MEDSA | 50.136 | 369 | 121 | 6 | 454 | 761 | 6 | 372 | 5.16e-108 | 337 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0004940 | Msa1007930 | 0.810448 | 1.147109e-50 | -8.615850e-47 |
| Msa0004990 | Msa1007930 | 0.813666 | 2.280972e-51 | -8.615850e-47 |
| Msa0011040 | Msa1007930 | 0.804924 | 1.710431e-49 | -8.615850e-47 |
| Msa0011510 | Msa1007930 | 0.804252 | 2.361597e-49 | -8.615850e-47 |
| Msa0012800 | Msa1007930 | 0.807285 | 5.447263e-50 | -8.615850e-47 |
| Msa0015070 | Msa1007930 | 0.837938 | 3.868709e-57 | -8.615850e-47 |
| Msa0017710 | Msa1007930 | 0.815211 | 1.038420e-51 | -8.615850e-47 |
| Msa0021870 | Msa1007930 | 0.818995 | 1.465350e-52 | -8.615850e-47 |
| Msa0024280 | Msa1007930 | 0.800318 | 1.524719e-48 | -8.615850e-47 |
| Msa0024650 | Msa1007930 | 0.840146 | 1.036637e-57 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1007930 | MtrunA17_Chr7g0218261 | 83.333 | 354 | 33 | 4 | 1 | 329 | 1 | 353 | 0.0 | 583 |
| Msa1007930 | MtrunA17_Chr7g0218261 | 67.397 | 365 | 57 | 6 | 456 | 761 | 7 | 368 | 4.61e-157 | 460 |
| Msa1007930 | MtrunA17_Chr7g0218211 | 80.274 | 365 | 11 | 4 | 456 | 761 | 7 | 369 | 0.0 | 563 |
| Msa1007930 | MtrunA17_Chr7g0218211 | 70.056 | 354 | 81 | 3 | 1 | 329 | 1 | 354 | 3.50e-173 | 501 |
| Msa1007930 | MtrunA17_Chr7g0218131 | 70.822 | 353 | 78 | 3 | 2 | 329 | 3 | 355 | 2.34e-179 | 517 |
| Msa1007930 | MtrunA17_Chr7g0218131 | 65.753 | 365 | 64 | 5 | 456 | 761 | 8 | 370 | 2.14e-154 | 453 |
| Msa1007930 | MtrunA17_Chr7g0218301 | 68.365 | 373 | 83 | 5 | 2 | 345 | 3 | 369 | 1.52e-170 | 495 |
| Msa1007930 | MtrunA17_Chr7g0218301 | 61.141 | 368 | 81 | 5 | 456 | 761 | 8 | 375 | 6.38e-143 | 424 |
| Msa1007930 | MtrunA17_Chr7g0218271 | 69.101 | 356 | 83 | 3 | 2 | 330 | 3 | 358 | 1.62e-170 | 495 |
| Msa1007930 | MtrunA17_Chr7g0218271 | 60.326 | 368 | 84 | 5 | 456 | 761 | 8 | 375 | 2.86e-142 | 422 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1007930 | AT5G54160.1 | 42.577 | 357 | 169 | 7 | 14 | 341 | 10 | 359 | 2.42e-83 | 270 |
| Msa1007930 | AT5G54160.1 | 37.677 | 353 | 153 | 8 | 469 | 761 | 16 | 361 | 3.46e-58 | 202 |
| Msa1007930 | AT1G51990.2 | 38.123 | 341 | 173 | 8 | 20 | 329 | 12 | 345 | 5.16e-69 | 231 |
| Msa1007930 | AT1G51990.2 | 30.878 | 353 | 180 | 8 | 469 | 760 | 12 | 361 | 1.76e-40 | 153 |
| Msa1007930 | AT1G51990.1 | 37.830 | 341 | 174 | 8 | 20 | 329 | 12 | 345 | 5.33e-68 | 229 |
| Msa1007930 | AT1G51990.1 | 30.878 | 353 | 180 | 8 | 469 | 760 | 12 | 361 | 9.02e-40 | 151 |
| Msa1007930 | AT1G77520.1 | 39.450 | 327 | 169 | 7 | 24 | 321 | 30 | 356 | 1.06e-67 | 228 |
| Msa1007930 | AT1G77520.1 | 30.894 | 369 | 185 | 9 | 460 | 761 | 16 | 381 | 8.98e-43 | 160 |
| Msa1007930 | AT1G33030.1 | 37.539 | 317 | 175 | 5 | 27 | 321 | 10 | 325 | 3.43e-60 | 207 |
| Msa1007930 | AT1G33030.1 | 32.092 | 349 | 166 | 11 | 476 | 761 | 10 | 350 | 9.58e-37 | 142 |
Find 172 sgRNAs with CRISPR-Local
Find 813 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATGACCGTGGCTATTTG+GGG | 0.651797 | 7_1:-7771185 | Msa1007930:CDS |
| AAACGATTACCTTGATAGGG+TGG | 0.654233 | 7_1:+7768283 | None:intergenic |
| AAATGGTAAGGTCATTATTG+TGG | 0.574360 | 7_1:-7767941 | Msa1007930:CDS |
| AAATTAATTGCCTTTATTGA+TGG | 0.347116 | 7_1:+7760678 | None:intergenic |
| AACACTCTGATTTGCCGAAT+AGG | 0.445684 | 7_1:-7761902 | Msa1007930:CDS |
| AACATACGATCGAGCCTATT+CGG | 0.337571 | 7_1:+7761888 | None:intergenic |
| AACATCATGTTTATCACAGC+TGG | 0.498404 | 7_1:-7767856 | Msa1007930:CDS |
| AACATGATGTTTGTCACAGC+TGG | 0.557381 | 7_1:-7759620 | Msa1007930:CDS |
| AACCAACAAATGTATCTCGA+TGG | 0.605786 | 7_1:-7759658 | Msa1007930:CDS |
| AACCGCAGGAAACACCATAT+TGG | 0.452926 | 7_1:+7762031 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATTCTCTTTCTCATTGGTAT+AGG | + | chr7_1:7759509-7759528 | None:intergenic | 30.0% | |
| ATCATTGTCATCTATTTGTG+GGG | + | chr7_1:7759551-7759570 | None:intergenic | 30.0% | |
| GTATCATTGTCATCTATTTG+TGG | + | chr7_1:7759553-7759572 | None:intergenic | 30.0% | |
| ATCATTGCTAAGGAAAGTAA+TGG | - | chr7_1:7759652-7759671 | Msa1007930:CDS | 30.0% | |
| CTAACAAAATATTTGCCAGA+TGG | + | chr7_1:7759844-7759863 | None:intergenic | 30.0% | |
| GTGTCAATTGCATTATTCTA+GGG | - | chr7_1:7760053-7760072 | Msa1007930:intron | 30.0% | |
| TATTATATATGCAAGGTCTG+GGG | + | chr7_1:7760138-7760157 | None:intergenic | 30.0% | |
| GTATTATATATGCAAGGTCT+GGG | + | chr7_1:7760139-7760158 | None:intergenic | 30.0% | |
| TGTATTATATATGCAAGGTC+TGG | + | chr7_1:7760140-7760159 | None:intergenic | 30.0% | |
| TAACAATGCATACGCATTAA+TGG | - | chr7_1:7760369-7760388 | Msa1007930:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_1 | gene | 7759502 | 7771574 | 7759502 | ID=Msa1007930;Name=Msa1007930 |
| chr7_1 | mRNA | 7759502 | 7771574 | 7759502 | ID=Msa1007930-mRNA-1;Parent=Msa1007930;Name=Msa1007930-mRNA-1;_AED=0.15;_eAED=0.15;_QI=0|0.23|0.07|1|0.46|0.14|14|0|761 |
| chr7_1 | exon | 7771369 | 7771574 | 7771369 | ID=Msa1007930-mRNA-1:exon:2331;Parent=Msa1007930-mRNA-1 |
| chr7_1 | exon | 7771141 | 7771335 | 7771141 | ID=Msa1007930-mRNA-1:exon:2330;Parent=Msa1007930-mRNA-1 |
| chr7_1 | exon | 7768555 | 7768606 | 7768555 | ID=Msa1007930-mRNA-1:exon:2329;Parent=Msa1007930-mRNA-1 |
| chr7_1 | exon | 7768293 | 7768521 | 7768293 | ID=Msa1007930-mRNA-1:exon:2328;Parent=Msa1007930-mRNA-1 |
| chr7_1 | exon | 7768137 | 7768201 | 7768137 | ID=Msa1007930-mRNA-1:exon:2327;Parent=Msa1007930-mRNA-1 |
| chr7_1 | exon | 7767818 | 7768034 | 7767818 | ID=Msa1007930-mRNA-1:exon:2326;Parent=Msa1007930-mRNA-1 |
| chr7_1 | exon | 7763826 | 7764040 | 7763826 | ID=Msa1007930-mRNA-1:exon:2325;Parent=Msa1007930-mRNA-1 |
| chr7_1 | exon | 7763672 | 7763727 | 7763672 | ID=Msa1007930-mRNA-1:exon:2324;Parent=Msa1007930-mRNA-1 |
| chr7_1 | exon | 7763488 | 7763621 | 7763488 | ID=Msa1007930-mRNA-1:exon:2323;Parent=Msa1007930-mRNA-1 |
| chr7_1 | exon | 7761891 | 7762125 | 7761891 | ID=Msa1007930-mRNA-1:exon:2322;Parent=Msa1007930-mRNA-1 |
| chr7_1 | exon | 7761708 | 7761872 | 7761708 | ID=Msa1007930-mRNA-1:exon:2321;Parent=Msa1007930-mRNA-1 |
| chr7_1 | exon | 7760899 | 7760950 | 7760899 | ID=Msa1007930-mRNA-1:exon:2320;Parent=Msa1007930-mRNA-1 |
| chr7_1 | exon | 7760637 | 7760865 | 7760637 | ID=Msa1007930-mRNA-1:exon:2319;Parent=Msa1007930-mRNA-1 |
| chr7_1 | exon | 7759502 | 7759737 | 7759502 | ID=Msa1007930-mRNA-1:exon:2318;Parent=Msa1007930-mRNA-1 |
| chr7_1 | CDS | 7771369 | 7771574 | 7771369 | ID=Msa1007930-mRNA-1:cds;Parent=Msa1007930-mRNA-1 |
| chr7_1 | CDS | 7771141 | 7771335 | 7771141 | ID=Msa1007930-mRNA-1:cds;Parent=Msa1007930-mRNA-1 |
| chr7_1 | CDS | 7768555 | 7768606 | 7768555 | ID=Msa1007930-mRNA-1:cds;Parent=Msa1007930-mRNA-1 |
| chr7_1 | CDS | 7768293 | 7768521 | 7768293 | ID=Msa1007930-mRNA-1:cds;Parent=Msa1007930-mRNA-1 |
| chr7_1 | CDS | 7768137 | 7768201 | 7768137 | ID=Msa1007930-mRNA-1:cds;Parent=Msa1007930-mRNA-1 |
| chr7_1 | CDS | 7767818 | 7768034 | 7767818 | ID=Msa1007930-mRNA-1:cds;Parent=Msa1007930-mRNA-1 |
| chr7_1 | CDS | 7763826 | 7764040 | 7763826 | ID=Msa1007930-mRNA-1:cds;Parent=Msa1007930-mRNA-1 |
| chr7_1 | CDS | 7763672 | 7763727 | 7763672 | ID=Msa1007930-mRNA-1:cds;Parent=Msa1007930-mRNA-1 |
| chr7_1 | CDS | 7763488 | 7763621 | 7763488 | ID=Msa1007930-mRNA-1:cds;Parent=Msa1007930-mRNA-1 |
| chr7_1 | CDS | 7761891 | 7762125 | 7761891 | ID=Msa1007930-mRNA-1:cds;Parent=Msa1007930-mRNA-1 |
| chr7_1 | CDS | 7761708 | 7761872 | 7761708 | ID=Msa1007930-mRNA-1:cds;Parent=Msa1007930-mRNA-1 |
| chr7_1 | CDS | 7760899 | 7760950 | 7760899 | ID=Msa1007930-mRNA-1:cds;Parent=Msa1007930-mRNA-1 |
| chr7_1 | CDS | 7760637 | 7760865 | 7760637 | ID=Msa1007930-mRNA-1:cds;Parent=Msa1007930-mRNA-1 |
| chr7_1 | CDS | 7759502 | 7759737 | 7759502 | ID=Msa1007930-mRNA-1:cds;Parent=Msa1007930-mRNA-1 |
| Gene Sequence |
>Msa1007930 ATGTCCTATACCAATGAGAAAGAGAATCATGTGGTAGAATCTCCCACCCCACAAATAGATGACAATGATACACTCTCAGCTATGGTACTTGGTGCAAATTTGGTGTTTCCTGCAGTTCTTAACGCTGCAATTGAACTCAATTTATTTGATATCATTGCTAAGGAAAGTAATGGTAGATTCTTGTCATCCTTTGAAATTGCTTCAAAGCTTGATCGTATGCTGCGCTTGCTTGCAAGCTACTCTCTTCTGTCTATTTCTACTCGGACCAATGATGCTGGTAAGATGGTGAGAATTTATGGTATCACACCATCTGGCAAATATTTTGTTAGTAATGAAAATGACCGTGGCTATTTGGGGTCAATGACTTCATATTTGTGTCACCCTGCTTTCTTAGGGGTGTGGTTGAATTTCAAGGATGCAGTTATGGATCCACAGATTGACCTATTCAAGAAAAAAGATCCAAAAATAAACCACATATTTAACAAATCAATGACTGATACATGTACACTACACATGAAAAGAATCCTTGAAATATACAAAGGATATGAGGGAATATCAACACTAGTTGATGCAGGAGGTGGAAATGGACAAAGTCTCAAAGCCATTATATCCAAATATCCATCAATAAAGGCAATCAACTTTGACCTCCCACAAGTGATTGAACATGCACCACCCTATCAAGGGATTGAACATTTTGGAGGAAATATGTTTGAATGTGTTCCACAAGGAGATGCTATAATGCTTAAGCTTGTGTGCCATAATTGGCCGGATGAAAAATGCTTAGAAATTTTAAGCAGTTGTCACAAAGCTTTGCCTCCAAATGGTAAGGTCATTATTGTGGAGTTCTTATCACCAGAAGATCTAGGATCAAAGAATGCATCTAAGATGGTTTCAATTGTGGACAACATCATGTTTATCACAGCTGGTGGTAAGGAAAGAACTCCAAAGGAATATGAGAGTTTAGATGGTTGGAGTGCATTGCGATTATTCTACGAATTTAGTGGAAGTAAGATTATTGCTTTCCAGTACCTTGGTGGAAGTAGATTTCAAATCTTTGTTTTTGCTCATAACATTACACTAGCTGATTATCCACCATATCATTCCATGAGTGCGGCATCATCATGTTATTGTACCTTTGAAGTTCCTATCGTTGGATATGGATCGACGAAATCACCGAAGGAACTACGTGATACTTTTGGCAAATTCCTTAGAGTGAAACATCAAGATTACGTCATACGACCCATACCAAATGTGATGTTCGGAAAAGGTTGGACGAGTTTTTGTGCTGCCAATGGTATTGAGGGCGGAGACGTGTTGATTTTCAAGTGCAAGTGCGTCATGGACAAAAAATATGTTATCGTATCCAAGCAGAATCATGTGTTGAAATCTCACACCCCACAAATAGATGACAATGATACACTCTCTGCTATGGTACTTGGTGCCAATATGGTGTTTCCTGCGGTTCTTAACGCTGCAATTGAACTCAATTTATTTGATATCATTGCTAAGGAAAGTTCACATAATGATGGATTCATGTCATCCTTTGAAATTGCTTCAAAGTTACCAACTCAACACTCTGATTTGCCGAATAGGCTCGATCGTATCTACTCTCTTCTGTCTGTTTCTACTCGGACCAATGATGATGGTAGCATAGTGAGAGTTTATGGGATCACACCATCTGGTAAATATTTTGTCAAGTATGAAAATGGTGATTCTTATTTGGGCTCATTCACATCATTTTTGTCTCACCCTGCTTTGTCAATGGTGTGGTTGAATTTTAAAGAAGCAATTATTGATCCAGAAGGGGACCTGTTCAAAAAAAAAGATCCACAGATAAATGAAATATTTAACAAATCAATGACGGATACATGCACAACTCACATGAAAAGAATCCTTGAAATATACCAAGGATTTGAGGGAATATCAACTTTGGTTGATGTAGGGGGTGGAACAGGACAAAGTCTCAAAATGATTATCTCCAAATACCCATCAATAAAGGCAATTAATTTTGACCTTCCACAAGTAATTGAAAATACACCACCTTGTCCAGCTTTGCCACCAAATGGAAAGCTCATTCTCATAGAGCCCTTGCAACCAGAAGATCTAGAACCAACAAATGTATCTCGATGGCTTTCAATTGCTGATAACATGATGTTTGTCACAGCTGGTGGAATGGAAAGAAGTGTGAAGGAATATGAGAGTTTGGGAAAACGATCTGGATTTTCTACGATTCAAGTTGTTTGTCTTGCTTTCTCTATCATTGGAGTGATGGAACTTTACAAATAA |
| Protein sequence |
>Msa1007930 MSYTNEKENHVVESPTPQIDDNDTLSAMVLGANLVFPAVLNAAIELNLFDIIAKESNGRFLSSFEIASKLDRMLRLLASYSLLSISTRTNDAGKMVRIYGITPSGKYFVSNENDRGYLGSMTSYLCHPAFLGVWLNFKDAVMDPQIDLFKKKDPKINHIFNKSMTDTCTLHMKRILEIYKGYEGISTLVDAGGGNGQSLKAIISKYPSIKAINFDLPQVIEHAPPYQGIEHFGGNMFECVPQGDAIMLKLVCHNWPDEKCLEILSSCHKALPPNGKVIIVEFLSPEDLGSKNASKMVSIVDNIMFITAGGKERTPKEYESLDGWSALRLFYEFSGSKIIAFQYLGGSRFQIFVFAHNITLADYPPYHSMSAASSCYCTFEVPIVGYGSTKSPKELRDTFGKFLRVKHQDYVIRPIPNVMFGKGWTSFCAANGIEGGDVLIFKCKCVMDKKYVIVSKQNHVLKSHTPQIDDNDTLSAMVLGANMVFPAVLNAAIELNLFDIIAKESSHNDGFMSSFEIASKLPTQHSDLPNRLDRIYSLLSVSTRTNDDGSIVRVYGITPSGKYFVKYENGDSYLGSFTSFLSHPALSMVWLNFKEAIIDPEGDLFKKKDPQINEIFNKSMTDTCTTHMKRILEIYQGFEGISTLVDVGGGTGQSLKMIISKYPSIKAINFDLPQVIENTPPCPALPPNGKLILIEPLQPEDLEPTNVSRWLSIADNMMFVTAGGMERSVKEYESLGKRSGFSTIQVVCLAFSIIGVMELYK* |
