AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1417220

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1417220 MtrunA17_Chr5g0404721 94.345 336 14 2 1 336 1 331 0.0 613
Msa1417220 MtrunA17_Chr8g0372601 80.997 321 56 2 15 335 15 330 0.0 534
Msa1417220 MtrunA17_Chr1g0155611 59.283 307 119 3 30 335 46 347 5.24e-121 352
Msa1417220 MtrunA17_Chr4g0013171 51.964 331 141 7 7 335 7 321 8.80e-106 312
Msa1417220 MtrunA17_Chr5g0438021 48.494 332 152 6 6 335 11 325 2.29e-104 309
Msa1417220 MtrunA17_Chr7g0258811 48.338 331 160 5 7 335 50 371 4.53e-104 310
Msa1417220 MtrunA17_Chr5g0432351 51.623 308 136 5 30 335 25 321 1.38e-103 306
Msa1417220 MtrunA17_Chr5g0400331 51.603 312 139 5 30 335 26 331 2.15e-103 306
Msa1417220 MtrunA17_Chr2g0320031 48.171 328 151 4 8 335 11 319 7.20e-103 305
Msa1417220 MtrunA17_Chr2g0320061 48.171 328 150 4 8 335 11 318 2.33e-102 303
Msa1417220 MtrunA17_Chr5g0432341 51.299 308 137 5 30 335 25 321 2.49e-102 303
Msa1417220 MtrunA17_Chr3g0114451 51.368 329 145 5 7 335 5 318 3.31e-102 303
Msa1417220 MtrunA17_Chr2g0292251 50.000 310 149 3 27 335 25 329 5.78e-102 304
Msa1417220 MtrunA17_Chr2g0320071 47.416 329 158 5 7 335 4 317 6.15e-102 302
Msa1417220 MtrunA17_Chr2g0292181 49.677 310 150 3 27 335 35 339 1.37e-101 303
Msa1417220 MtrunA17_Chr2g0320051 49.032 310 146 3 26 335 21 318 1.87e-101 301
Msa1417220 MtrunA17_Chr3g0106921 49.515 309 140 6 30 335 25 320 4.66e-101 300
Msa1417220 MtrunA17_Chr2g0292241 49.839 311 150 3 27 336 26 331 8.03e-101 301
Msa1417220 MtrunA17_Chr2g0299691 50.645 310 142 4 30 335 56 358 2.45e-100 300
Msa1417220 MtrunA17_Chr5g0432331 50.325 308 140 5 30 335 56 352 1.07e-99 298
Msa1417220 MtrunA17_Chr3g0129051 46.970 330 157 6 8 335 9 322 4.40e-99 295
Msa1417220 MtrunA17_Chr4g0013181 52.961 304 128 6 34 335 63 353 9.00e-99 295
Msa1417220 MtrunA17_Chr1g0175561 45.697 337 169 5 1 335 2 326 9.11e-99 295
Msa1417220 MtrunA17_Chr2g0292111 49.677 310 150 3 27 335 26 330 9.90e-99 295
Msa1417220 MtrunA17_Chr5g0432441 49.254 335 145 8 8 335 10 326 1.26e-98 294
Msa1417220 MtrunA17_Chr2g0291751 48.276 319 154 4 18 335 22 330 1.34e-98 295
Msa1417220 MtrunA17_Chr2g0292091 49.196 311 150 4 28 335 20 325 8.22e-98 293
Msa1417220 MtrunA17_Chr2g0292211 49.032 310 152 3 27 335 37 341 1.50e-97 293
Msa1417220 MtrunA17_Chr2g0320101 47.112 329 159 5 7 335 4 317 2.14e-97 291
Msa1417220 MtrunA17_Chr4g0043391 45.266 338 165 5 1 335 4 324 4.99e-97 290
Msa1417220 MtrunA17_Chr2g0292101 48.875 311 151 4 28 335 21 326 1.36e-96 290
Msa1417220 MtrunA17_Chr7g0244461 47.419 310 150 4 26 335 16 312 1.36e-96 288
Msa1417220 MtrunA17_Chr3g0129061 47.879 330 149 6 8 335 14 322 2.31e-96 288
Msa1417220 MtrunA17_Chr2g0292151 48.065 310 155 3 27 335 26 330 2.53e-96 289
Msa1417220 MtrunA17_Chr4g0070951 50.485 309 143 4 30 335 28 329 3.84e-96 288
Msa1417220 MtrunA17_Chr5g0402571 48.485 330 156 6 7 335 10 326 4.43e-96 288
Msa1417220 MtrunA17_Chr4g0068621 48.860 307 148 4 30 335 38 336 4.46e-96 288
Msa1417220 MtrunA17_Chr4g0038171 44.675 338 167 5 1 335 1 321 1.47e-95 286
Msa1417220 MtrunA17_Chr2g0320091 46.646 328 158 7 9 335 7 318 4.97e-95 285
Msa1417220 MtrunA17_Chr2g0292271 45.732 328 166 4 9 335 14 330 9.01e-95 285
Msa1417220 MtrunA17_Chr4g0038161 46.061 330 157 6 9 335 9 320 9.43e-95 284
Msa1417220 MtrunA17_Chr4g0038151 47.115 312 150 5 27 335 22 321 1.26e-94 284
Msa1417220 MtrunA17_Chr3g0129041 48.024 329 149 6 9 335 14 322 2.65e-94 283
Msa1417220 MtrunA17_Chr7g0244451 44.970 338 156 6 2 335 5 316 2.66e-93 280
Msa1417220 MtrunA17_Chr5g0402581 47.273 330 160 6 7 335 10 326 1.33e-92 279
Msa1417220 MtrunA17_Chr3g0107881 43.731 327 170 5 11 335 10 324 7.60e-92 277
Msa1417220 MtrunA17_Chr4g0013191 47.419 310 145 7 30 335 45 340 1.37e-91 277
Msa1417220 MtrunA17_Chr2g0292261 48.185 303 151 3 34 335 33 330 3.93e-91 276
Msa1417220 MtrunA17_Chr7g0224371 48.039 306 137 6 34 335 35 322 1.70e-90 273
Msa1417220 MtrunA17_Chr2g0292291 44.411 331 170 5 14 335 7 332 1.00e-89 272
Msa1417220 MtrunA17_Chr4g0013201 45.277 307 153 5 30 335 46 338 1.00e-88 269
Msa1417220 MtrunA17_Chr3g0106901 45.897 329 156 5 7 335 17 323 2.85e-88 268
Msa1417220 MtrunA17_Chr4g0013721 45.928 307 157 5 30 336 100 397 1.13e-86 266
Msa1417220 MtrunA17_Chr1g0213061 42.988 328 170 6 10 335 9 321 1.72e-85 261
Msa1417220 MtrunA17_Chr5g0421411 45.993 287 146 5 53 335 4 285 4.05e-85 258
Msa1417220 MtrunA17_Chr4g0013821 44.118 306 162 5 30 335 28 324 5.62e-85 260
Msa1417220 MtrunA17_Chr2g0322861 44.083 338 173 8 1 336 64 387 9.61e-84 258
Msa1417220 MtrunA17_Chr4g0024121 45.603 307 159 5 30 335 27 326 4.16e-82 252
Msa1417220 MtrunA17_Chr7g0268501 43.807 331 173 6 5 335 3 320 3.15e-81 249
Msa1417220 MtrunA17_Chr7g0268521 43.807 331 173 6 5 335 3 320 3.15e-81 249
Msa1417220 MtrunA17_Chr3g0128061 45.215 303 159 3 34 335 30 326 3.81e-79 244
Msa1417220 MtrunA17_Chr1g0194151 44.408 304 163 3 34 335 34 333 5.86e-79 244
Msa1417220 MtrunA17_Chr2g0279041 44.083 338 162 9 7 335 6 325 9.01e-78 241
Msa1417220 MtrunA17_Chr1g0194271 43.087 311 164 4 34 335 34 340 2.39e-76 238
Msa1417220 MtrunA17_Chr1g0164891 43.421 304 166 4 34 335 93 392 3.01e-76 239
Msa1417220 MtrunA17_Chr4g0076211 42.715 302 158 7 35 335 46 333 1.22e-74 233
Msa1417220 MtrunA17_Chr1g0204011 40.606 330 178 8 7 335 10 322 4.60e-74 231
Msa1417220 MtrunA17_Chr2g0296621 43.408 311 160 9 26 335 46 341 1.38e-73 231
Msa1417220 MtrunA17_Chr5g0432401 40.645 310 132 5 27 334 26 285 5.00e-73 227
Msa1417220 MtrunA17_Chr5g0405991 40.896 335 176 7 1 335 4 316 1.07e-72 228
Msa1417220 MtrunA17_Chr6g0460711 42.053 302 163 6 34 335 25 314 3.89e-72 226
Msa1417220 MtrunA17_Chr8g0371381 42.105 304 160 7 34 335 30 319 2.35e-70 221
Msa1417220 MtrunA17_Chr4g0063671 39.706 340 180 8 1 335 1 320 2.53e-70 221
Msa1417220 MtrunA17_Chr1g0181511 41.503 306 162 6 34 335 31 323 3.76e-70 221
Msa1417220 MtrunA17_Chr6g0477071 40.397 302 163 7 34 335 58 342 1.19e-69 221
Msa1417220 MtrunA17_Chr7g0254231 39.482 309 171 6 28 336 20 312 5.66e-69 218
Msa1417220 MtrunA17_Chr8g0364291 39.514 329 187 5 10 336 7 325 7.15e-68 216
Msa1417220 MtrunA17_Chr1g0188431 38.720 328 184 7 8 335 17 327 1.18e-67 215
Msa1417220 MtrunA17_Chr4g0046131 37.941 340 190 7 7 331 9 342 9.87e-66 211
Msa1417220 MtrunA17_Chr7g0254201 40.397 302 167 7 34 335 24 312 1.30e-65 209
Msa1417220 MtrunA17_Chr7g0225221 40.391 307 171 5 30 335 25 320 1.61e-65 209
Msa1417220 MtrunA17_Chr1g0181431 39.672 305 169 5 34 335 31 323 2.02e-65 209
Msa1417220 MtrunA17_Chr5g0401431 38.369 331 192 7 8 334 6 328 3.14e-65 209
Msa1417220 MtrunA17_Chr1g0157051 38.671 331 186 7 1 331 1 314 1.21e-64 207
Msa1417220 MtrunA17_Chr1g0181481 39.672 305 169 5 34 335 31 323 1.01e-63 205
Msa1417220 MtrunA17_Chr1g0148021 38.623 334 190 6 5 335 7 328 3.24e-63 204
Msa1417220 MtrunA17_Chr4g0075241 35.135 333 206 5 4 335 4 327 1.43e-62 202
Msa1417220 MtrunA17_Chr2g0278671 41.214 313 164 10 30 335 24 323 1.64e-62 201
Msa1417220 MtrunA17_Chr4g0072091 37.908 306 176 6 34 335 28 323 2.20e-61 199
Msa1417220 MtrunA17_Chr2g0316481 35.714 350 185 8 18 334 63 405 2.52e-61 201
Msa1417220 MtrunA17_Chr2g0316791 36.080 352 181 9 18 334 63 405 3.44e-61 201
Msa1417220 MtrunA17_Chr1g0197411 35.329 334 203 6 5 335 7 330 4.00e-61 198
Msa1417220 MtrunA17_Chr6g0467251 37.015 335 191 8 5 336 12 329 4.03e-61 199
Msa1417220 MtrunA17_Chr2g0329641 36.257 342 194 10 5 335 7 335 1.16e-60 197
Msa1417220 MtrunA17_Chr6g0467121 35.652 345 190 10 2 335 40 363 1.57e-60 198
Msa1417220 MtrunA17_Chr2g0316831 36.000 350 184 9 18 334 99 441 2.16e-60 199
Msa1417220 MtrunA17_Chr6g0467161 34.226 336 196 7 8 335 18 336 4.29e-60 196
Msa1417220 MtrunA17_Chr4g0074851 35.758 330 194 6 8 331 7 324 8.10e-60 195
Msa1417220 MtrunA17_Chr2g0279061 45.489 266 130 7 74 335 12 266 1.27e-59 192
Msa1417220 MtrunA17_Chr3g0136751 39.672 305 170 5 34 335 29 322 2.55e-59 193
Msa1417220 MtrunA17_Chr4g0053871 37.994 329 188 7 6 331 5 320 2.91e-59 193
Msa1417220 MtrunA17_Chr2g0311241 32.902 386 187 9 18 334 47 429 7.56e-59 195
Msa1417220 MtrunA17_Chr2g0316191 34.473 351 189 8 18 334 63 406 7.74e-59 194
Msa1417220 MtrunA17_Chr6g0451571 33.735 332 203 7 7 335 17 334 4.66e-57 188
Msa1417220 MtrunA17_Chr5g0413051 35.669 314 189 7 28 335 71 377 1.48e-55 185
Msa1417220 MtrunA17_Chr2g0311251 34.535 333 178 8 34 334 15 339 3.31e-53 178
Msa1417220 MtrunA17_Chr3g0114461 50.000 182 84 1 154 335 18 192 4.23e-52 171
Msa1417220 MtrunA17_Chr2g0292281 42.326 215 106 5 123 335 18 216 1.74e-42 147
Msa1417220 MtrunA17_Chr3g0114491 60.714 112 44 0 30 141 18 129 1.66e-41 141
Msa1417220 MtrunA17_Chr2g0316811 37.037 243 129 5 18 242 37 273 2.63e-41 147
Msa1417220 MtrunA17_Chr4g0004591 28.660 321 163 5 34 334 81 355 2.83e-35 131
Msa1417220 MtrunA17_Chr2g0316141 29.932 294 148 9 76 336 11 279 5.79e-32 120
Msa1417220 MtrunA17_Chr2g0316181 29.932 294 148 9 76 336 11 279 5.79e-32 120
Msa1417220 MtrunA17_Chr1g0181501 36.413 184 107 4 155 335 13 189 6.51e-32 118
Msa1417220 MtrunA17_Chr5g0432321 46.324 136 64 3 144 279 6 132 1.11e-29 110
Msa1417220 MtrunA17_Chr3g0104101 41.135 141 79 3 199 335 9 149 7.49e-26 100
Msa1417220 MtrunA17_Chr1g0181491 46.729 107 55 2 34 140 16 120 1.75e-23 94.7
Msa1417220 MtrunA17_Chr6g0468731 62.162 74 24 2 69 142 22 91 6.16e-22 88.6
Msa1417220 MtrunA17_Chr5g0432361 42.056 107 55 3 229 334 8 108 7.04e-18 78.2
Msa1417220 MtrunA17_Chr7g0268491 50.667 75 35 2 261 335 25 97 8.10e-16 72.0
Msa1417220 MtrunA17_Chr1g0196191 53.425 73 33 1 72 144 27 98 7.86e-15 71.2
Msa1417220 MtrunA17_Chr4g0013811 49.333 75 34 1 145 219 9 79 1.12e-14 68.6
Msa1417220 MtrunA17_Chr7g0254181 36.207 116 61 3 91 201 15 122 5.10e-13 66.2
Msa1417220 MtrunA17_Chr7g0254171 43.662 71 39 1 75 145 3 72 9.19e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1417220 AT5G66390.1 71.976 339 87 4 1 336 1 334 0.0 504
Msa1417220 AT4G36430.1 67.183 323 101 2 14 336 14 331 1.57e-160 452
Msa1417220 AT2G18150.1 65.476 336 105 3 7 336 7 337 4.34e-160 451
Msa1417220 AT2G18140.1 61.607 336 119 3 6 336 6 336 1.36e-147 419
Msa1417220 AT3G50990.1 61.934 331 116 3 11 336 17 342 1.92e-144 412
Msa1417220 AT1G44970.1 63.192 307 107 3 30 335 45 346 2.62e-130 376
Msa1417220 AT2G35380.1 51.757 313 137 5 30 335 30 335 4.45e-103 306
Msa1417220 AT5G06720.1 48.860 307 151 3 30 335 32 333 2.83e-102 304
Msa1417220 AT5G05340.1 47.756 312 148 5 26 335 26 324 6.77e-99 295
Msa1417220 AT1G14550.1 46.809 329 157 5 8 335 10 321 7.33e-98 292
Msa1417220 AT4G08770.1 47.742 310 154 3 30 336 24 328 1.25e-96 290
Msa1417220 AT5G19890.1 49.541 327 151 6 13 336 13 328 1.61e-96 289
Msa1417220 AT4G08780.1 47.588 311 153 4 30 336 24 328 2.23e-96 290
Msa1417220 AT5G58400.1 44.578 332 162 7 7 335 13 325 2.09e-95 286
Msa1417220 AT5G06730.1 45.603 307 161 3 30 335 33 334 3.59e-95 287
Msa1417220 AT1G49570.1 50.662 302 140 5 33 331 51 346 2.97e-94 284
Msa1417220 AT3G32980.1 47.419 310 155 4 30 336 31 335 9.79e-94 283
Msa1417220 AT3G49110.1 46.855 318 161 4 22 336 25 337 1.58e-93 283
Msa1417220 AT2G38390.1 45.455 308 161 4 30 335 31 333 3.82e-93 281
Msa1417220 AT4G16270.1 49.175 303 145 5 34 335 54 348 1.05e-92 280
Msa1417220 AT3G49120.1 47.097 310 156 4 30 336 32 336 1.75e-90 275
Msa1417220 AT2G38380.1 45.455 308 161 4 30 335 31 333 7.02e-89 270
Msa1417220 AT5G58390.1 44.611 334 163 7 5 335 2 316 5.74e-88 267
Msa1417220 AT1G14540.1 42.857 329 168 3 7 335 7 315 6.26e-88 267
Msa1417220 AT2G22420.1 46.078 306 156 5 30 335 23 319 1.35e-87 266
Msa1417220 AT1G68850.1 42.090 335 183 6 7 335 6 335 1.27e-85 261
Msa1417220 AT3G03670.1 41.945 329 178 4 7 335 6 321 4.20e-83 254
Msa1417220 AT5G19880.1 39.759 332 185 5 8 335 9 329 4.36e-79 244
Msa1417220 AT5G15180.1 44.702 302 159 5 34 335 36 329 8.34e-79 244
Msa1417220 AT4G11290.1 43.750 304 163 5 34 335 29 326 7.32e-77 239
Msa1417220 AT5G64120.1 41.722 302 164 7 34 335 39 328 1.81e-76 238
Msa1417220 AT1G05260.1 40.419 334 189 6 5 336 1 326 2.86e-75 235
Msa1417220 AT2G39040.1 43.438 320 166 7 23 335 39 350 3.59e-75 235
Msa1417220 AT5G17820.1 38.806 335 183 7 1 335 1 313 1.24e-73 230
Msa1417220 AT1G71695.1 40.698 344 177 10 5 335 10 339 5.45e-73 230
Msa1417220 AT5G17820.2 38.806 335 183 7 1 335 54 366 1.69e-72 229
Msa1417220 AT3G01190.1 42.638 326 171 7 10 335 12 321 2.79e-72 227
Msa1417220 AT2G35380.2 48.031 254 118 5 89 335 1 247 3.66e-72 224
Msa1417220 AT1G05240.1 41.722 302 162 7 34 331 30 321 3.78e-72 227
Msa1417220 AT1G05250.1 41.722 302 162 7 34 331 30 321 3.78e-72 227
Msa1417220 AT3G21770.1 42.071 309 165 6 27 331 26 324 1.12e-71 226
Msa1417220 AT1G30870.1 43.631 314 160 8 26 336 47 346 3.27e-71 225
Msa1417220 AT5G42180.1 42.295 305 165 5 30 334 23 316 9.75e-71 223
Msa1417220 AT2G41480.1 42.020 307 162 6 34 335 33 328 5.42e-70 221
Msa1417220 AT5G51890.1 38.739 333 181 10 7 335 9 322 6.35e-70 221
Msa1417220 AT5G39580.1 41.584 303 164 7 34 335 29 319 1.84e-69 219
Msa1417220 AT2G37130.1 40.064 312 173 5 27 335 27 327 9.68e-69 218
Msa1417220 AT2G37130.2 40.328 305 168 5 34 335 3 296 1.49e-68 216
Msa1417220 AT5G47000.1 37.349 332 193 7 7 336 13 331 2.11e-66 212
Msa1417220 AT5G64100.1 41.776 304 162 6 34 335 41 331 2.70e-66 212
Msa1417220 AT4G33870.1 36.176 340 175 6 29 333 67 399 6.39e-66 213
Msa1417220 AT1G24110.1 39.032 310 178 6 30 336 22 323 5.60e-65 208
Msa1417220 AT4G26010.1 39.009 323 177 8 15 336 7 310 1.30e-64 207
Msa1417220 AT4G26010.2 39.009 323 177 8 15 336 35 338 1.69e-64 207
Msa1417220 AT4G37530.1 38.188 309 182 4 30 335 27 329 1.28e-63 205
Msa1417220 AT5G22410.1 37.463 339 191 10 2 336 5 326 1.79e-63 204
Msa1417220 AT5G64110.1 40.656 305 166 7 34 335 38 330 4.73e-63 204
Msa1417220 AT5G14130.1 36.474 329 191 6 8 335 19 330 5.04e-63 203
Msa1417220 AT3G28200.1 37.662 308 178 6 30 335 21 316 1.13e-62 202
Msa1417220 AT4G37520.1 37.217 309 185 4 30 335 27 329 1.30e-62 202
Msa1417220 AT5G14130.2 36.474 329 191 6 8 335 60 371 1.84e-62 203
Msa1417220 AT2G18980.1 36.578 339 195 7 1 335 1 323 1.45e-61 199
Msa1417220 AT4G33420.1 38.024 334 184 9 2 335 15 325 2.05e-61 199
Msa1417220 AT4G37520.2 36.570 309 184 4 30 335 27 326 2.64e-61 199
Msa1417220 AT2G34060.1 36.607 336 197 7 7 332 14 343 3.08e-61 199
Msa1417220 AT2G24800.1 39.672 305 171 6 34 335 35 329 5.13e-61 198
Msa1417220 AT4G21960.1 38.033 305 174 5 34 335 35 327 5.56e-61 198
Msa1417220 AT4G33420.2 38.024 334 184 9 2 335 62 372 6.38e-61 199
Msa1417220 AT1G77100.1 38.961 308 166 9 34 335 28 319 6.63e-61 197
Msa1417220 AT4G17690.1 37.338 308 180 6 30 335 26 322 7.32e-61 198
Msa1417220 AT4G25980.1 37.134 307 178 6 34 335 30 326 7.73e-61 197
Msa1417220 AT5G40150.1 36.859 312 184 6 26 335 28 328 8.35e-61 197
Msa1417220 AT4G30170.1 36.825 315 182 7 26 335 23 325 1.48e-60 197
Msa1417220 AT1G34510.1 37.888 322 180 8 16 336 8 310 1.61e-60 196
Msa1417220 AT4G31760.1 38.158 304 176 5 34 335 33 326 3.43e-60 196
Msa1417220 AT5G67400.1 37.540 309 184 4 30 335 27 329 9.31e-59 192
Msa1417220 AT3G49960.1 36.393 305 185 4 34 335 31 329 1.61e-56 187
Msa1417220 AT2G43480.1 37.171 304 174 7 37 335 44 335 8.28e-55 182
Msa1417220 AT4G37530.2 38.976 254 147 4 34 284 31 279 1.52e-53 177
Msa1417220 AT5G24070.1 32.849 344 206 8 5 335 4 335 2.90e-53 179
Msa1417220 AT3G17070.1 32.849 344 190 11 13 335 16 339 9.20e-50 169
Msa1417220 AT4G25980.2 43.195 169 87 4 34 202 75 234 7.95e-38 137
Msa1417220 AT5G39580.2 43.182 176 90 4 34 209 29 194 2.50e-36 131
Msa1417220 AT2G24800.2 42.857 168 87 2 34 201 35 193 2.59e-34 126
Msa1417220 AT3G17070.2 31.541 279 156 10 72 335 18 276 4.98e-33 124

Find 79 sgRNAs with CRISPR-Local

Find 240 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCACCCCTTGATCCTGTTAA+AGG 0.173311 tig0024455:+13171 None:intergenic
CTTGACAATCTCTTCAACTT+TGG 0.243603 tig0024455:+14601 None:intergenic
AGTCTAGGACAAACAGATTT+TGG 0.271787 tig0024455:+13385 None:intergenic
CAACTACTACAAGAACTTAT+TGG 0.285025 tig0024455:-13337 Msa1417220:CDS
TAATAAGATGTTTCAAGAAT+TGG 0.295395 tig0024455:-12945 Msa1417220:three_prime_UTR
CTGACTTGCCATTCCCAGTT+TGG 0.295882 tig0024455:+13472 None:intergenic
ATGGTTTGGAATGTATTGTT+AGG 0.303652 tig0024455:+13849 None:intergenic
GTTGATCTAGTTGCTCTATC+TGG 0.307619 tig0024455:-13792 Msa1417220:intron
GGAAACATTACTCCTTTAAC+AGG 0.313337 tig0024455:-13183 Msa1417220:CDS
TGTGTCCTGAGTATAAAATT+TGG 0.318039 tig0024455:-13010 Msa1417220:three_prime_UTR
CTTAACTAAATTCAAGCTTA+AGG 0.333245 tig0024455:-13826 Msa1417220:CDS
TGAATTTAGTTAAGATGGTT+TGG 0.336203 tig0024455:+13835 None:intergenic
TTGACAATCTCTTCAACTTT+GGG 0.338379 tig0024455:+14602 None:intergenic
CAAAGACTCTACAACCAAAC+TGG 0.339170 tig0024455:-13486 Msa1417220:CDS
TTAACTAAATTCAAGCTTAA+GGG 0.348519 tig0024455:-13825 Msa1417220:CDS
CAAATCCTCTAGCTGAATTA+CGG 0.358640 tig0024455:+14147 None:intergenic
GAGTATAAAATTTGGGCTTT+TGG 0.362183 tig0024455:-13002 Msa1417220:three_prime_UTR
GTGTCCTGAGTATAAAATTT+GGG 0.365686 tig0024455:-13009 Msa1417220:three_prime_UTR
CGCACTCGGTGCCCAAGATC+TGG 0.377599 tig0024455:-13414 Msa1417220:CDS
CCTCTAGCTGAATTACGGTT+TGG 0.384144 tig0024455:+14152 None:intergenic
AAGCTTGAATTTAGTTAAGA+TGG 0.387881 tig0024455:+13830 None:intergenic
GCAATTGAGCTGCATAGTTT+TGG 0.388025 tig0024455:+13436 None:intergenic
TATCAGCACAAGAGACTGTC+TGG 0.410605 tig0024455:+14081 None:intergenic
TTTGGTCTCCACCAGATCTT+GGG 0.428573 tig0024455:+13403 None:intergenic
TCAGACTGGTGGACCAAGTT+GGG 0.428730 tig0024455:-13939 Msa1417220:intron
TGCCAAGTCCATGGTTAAGA+TGG 0.429935 tig0024455:-13205 Msa1417220:CDS
ACCAAGTTGGGATGTACCTT+TGG 0.431284 tig0024455:-13927 Msa1417220:CDS
TGGTTTGGAATGTATTGTTA+GGG 0.440392 tig0024455:+13850 None:intergenic
TCCCATCTTAACCATGGACT+TGG 0.441177 tig0024455:+13203 None:intergenic
ACAGAATTTCATCAGAACTT+AGG 0.456756 tig0024455:+13301 None:intergenic
CAGCAAGGAAGCAGCCATGC+GGG 0.463288 tig0024455:+14547 None:intergenic
GTAGAGTCTTTGTCTAAAGC+TGG 0.469738 tig0024455:+13497 None:intergenic
TTACTCCTTTAACAGGATCA+AGG 0.477963 tig0024455:-13176 Msa1417220:CDS
TGGAATGTATTGTTAGGGGC+AGG 0.478296 tig0024455:+13855 None:intergenic
ATGCAGCTCAATTGCGCACT+CGG 0.490653 tig0024455:-13428 Msa1417220:CDS
TCTCTTGGTGCAAGCATAAG+TGG 0.493124 tig0024455:-13894 Msa1417220:CDS
CAAGAACTTATTGGCCAACA+AGG 0.495886 tig0024455:-13328 Msa1417220:CDS
CACGAATCATCGTAAAATTG+AGG 0.496099 tig0024455:+14626 None:intergenic
CTCTACAACCAAACTGGGAA+TGG 0.498579 tig0024455:-13480 Msa1417220:CDS
AGAACTTAGGAGACCCTTGT+TGG 0.510984 tig0024455:+13314 None:intergenic
TTTGGAAGAAGAGATTCTCT+TGG 0.512677 tig0024455:-13909 Msa1417220:CDS
TACTCCTTTAACAGGATCAA+GGG 0.516424 tig0024455:-13175 Msa1417220:CDS
GGTGCACCGCGAATCTCCTA+TGG 0.523145 tig0024455:+13518 None:intergenic
ATTCAATCCTTTCAGACTGG+TGG 0.533760 tig0024455:-13950 Msa1417220:intron
AGCGACAGCCTTAGCAACTA+CGG 0.534152 tig0024455:+14577 None:intergenic
TTCAGACTGGTGGACCAAGT+TGG 0.534896 tig0024455:-13940 Msa1417220:intron
GCCAAGTCCATGGTTAAGAT+GGG 0.535394 tig0024455:-13204 Msa1417220:CDS
TGAACAATTTGCCAAGTCCA+TGG 0.536039 tig0024455:-13214 Msa1417220:CDS
TCCAAAGGTACATCCCAACT+TGG 0.541037 tig0024455:+13926 None:intergenic
TGTCAAGTCCGTAGTTGCTA+AGG 0.541793 tig0024455:-14585 Msa1417220:CDS
CATCGTAAAATTGAGGGTAG+AGG 0.558315 tig0024455:+14633 None:intergenic
ACGAATCATCGTAAAATTGA+GGG 0.561360 tig0024455:+14627 None:intergenic
TTACCGGTGTGACAAAGTCT+AGG 0.570159 tig0024455:+13370 None:intergenic
CCAAACCGTAATTCAGCTAG+AGG 0.570737 tig0024455:-14152 Msa1417220:CDS
GAACAATCATCAGCGAAAAG+AGG 0.590852 tig0024455:-14181 Msa1417220:CDS
AATGTTTCCCATCTTAACCA+TGG 0.593838 tig0024455:+13197 None:intergenic
AGCAAGGAAGCAGCCATGCG+GGG 0.595766 tig0024455:+14548 None:intergenic
ATACCTTGACAAAACAGTCA+TGG 0.599142 tig0024455:+14513 None:intergenic
ATCAGCACAAGAGACTGTCT+GGG 0.599732 tig0024455:+14082 None:intergenic
GTTATAGGTAGCCACACCAT+AGG 0.605653 tig0024455:-13534 Msa1417220:intron
GCCACACCATAGGAGATTCG+CGG 0.611411 tig0024455:-13524 Msa1417220:CDS
AAGAGAATCTCTTCTTCCAA+AGG 0.614698 tig0024455:+13911 None:intergenic
TGTCGCTAAAGAACCCCGCA+TGG 0.629260 tig0024455:-14561 Msa1417220:CDS
ACCGCGAATCTCCTATGGTG+TGG 0.629403 tig0024455:+13523 None:intergenic
GGGACACTCTTTCTCTACAG+CGG 0.636108 tig0024455:+14103 None:intergenic
AAAGACTCTACAACCAAACT+GGG 0.636762 tig0024455:-13485 Msa1417220:CDS
ACTCGGTGCCCAAGATCTGG+TGG 0.639184 tig0024455:-13411 Msa1417220:CDS
TGTCCTAGACTTTGTCACAC+CGG 0.643380 tig0024455:-13373 Msa1417220:CDS
GGACACAGCGACTATGCCAA+TGG 0.644492 tig0024455:+13026 None:intergenic
ACTCCTTTAACAGGATCAAG+GGG 0.648428 tig0024455:-13174 Msa1417220:CDS
ATGGAAATGTAGTCTCAGCA+AGG 0.653675 tig0024455:+14532 None:intergenic
TCAGCAAGGAAGCAGCCATG+CGG 0.656575 tig0024455:+14546 None:intergenic
AAATACTATCATTTCCACCA+TGG 0.660518 tig0024455:-14801 Msa1417220:five_prime_UTR
GGTTTGGAATGTATTGTTAG+GGG 0.668824 tig0024455:+13851 None:intergenic
AAGAACTTATTGGCCAACAA+GGG 0.679001 tig0024455:-13327 Msa1417220:CDS
TCAGTGTTGCTAGATAGCAG+TGG 0.688736 tig0024455:-14203 Msa1417220:CDS
TCAGCACAAGAGACTGTCTG+GGG 0.742569 tig0024455:+14083 None:intergenic
GGATTGGATGAATACCATGG+TGG 0.761315 tig0024455:+14787 None:intergenic
ACTGGATTGGATGAATACCA+TGG 0.762767 tig0024455:+14784 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTAAACAATGTTTAAAAATG+TGG - tig0024455:13254-13273 Msa1417220:CDS 20.0%
!!! TGTTATTTTTTAAAACCTTG+TGG - tig0024455:13361-13380 Msa1417220:CDS 20.0%
!!! ACTTGATATTTAACTTTTGA+TGG + tig0024455:13759-13778 None:intergenic 20.0%
!! TTAACTAAATTCAAGCTTAA+GGG - tig0024455:13909-13928 Msa1417220:CDS 20.0%
!! GAAGAAAATTGAGATAAATT+TGG + tig0024455:14123-14142 None:intergenic 20.0%
!!! AATTTTCTTCATTTGAGTTT+TGG - tig0024455:14133-14152 Msa1417220:CDS 20.0%
!! ACAAATTCTTGTAATGTTAT+AGG - tig0024455:14185-14204 Msa1417220:CDS 20.0%
!! TAATAAGATGTTTCAAGAAT+TGG - tig0024455:14789-14808 Msa1417220:five_prime_UTR 20.0%
!! GTAAACAATGTTTAAAAATG+TGG - tig0024455:13254-13273 Msa1417220:CDS 20.0%
!!! TGTTATTTTTTAAAACCTTG+TGG - tig0024455:13361-13380 Msa1417220:CDS 20.0%
!!! ACTTGATATTTAACTTTTGA+TGG + tig0024455:13759-13778 None:intergenic 20.0%
!! TTAACTAAATTCAAGCTTAA+GGG - tig0024455:13909-13928 Msa1417220:CDS 20.0%
!! GAAGAAAATTGAGATAAATT+TGG + tig0024455:14123-14142 None:intergenic 20.0%
!!! AATTTTCTTCATTTGAGTTT+TGG - tig0024455:14133-14152 Msa1417220:CDS 20.0%
!! ACAAATTCTTGTAATGTTAT+AGG - tig0024455:14185-14204 Msa1417220:CDS 20.0%
!! TAATAAGATGTTTCAAGAAT+TGG - tig0024455:14789-14808 Msa1417220:five_prime_UTR 20.0%
! TGATCTTGAAAGAAAAACAA+TGG - tig0024455:12996-13015 Msa1417220:three_prime_UTR 25.0%
! AATGTTTAAAAATGTGGTCA+TGG - tig0024455:13260-13279 Msa1417220:CDS 25.0%
! TGATCTATGAGTTGTATATT+GGG - tig0024455:13424-13443 Msa1417220:CDS 25.0%
! TGAATTTAGTTAAGATGGTT+TGG + tig0024455:13902-13921 None:intergenic 25.0%
! AAGCTTGAATTTAGTTAAGA+TGG + tig0024455:13907-13926 None:intergenic 25.0%
! CTTAACTAAATTCAAGCTTA+AGG - tig0024455:13908-13927 Msa1417220:CDS 25.0%
! AGATGAAATAGATTAGTACT+AGG + tig0024455:13974-13993 None:intergenic 25.0%
! TGATCTTGAAAGAAAAACAA+TGG - tig0024455:12996-13015 Msa1417220:three_prime_UTR 25.0%
! AATGTTTAAAAATGTGGTCA+TGG - tig0024455:13260-13279 Msa1417220:CDS 25.0%
! TGATCTATGAGTTGTATATT+GGG - tig0024455:13424-13443 Msa1417220:CDS 25.0%
! TGAATTTAGTTAAGATGGTT+TGG + tig0024455:13902-13921 None:intergenic 25.0%
! AAGCTTGAATTTAGTTAAGA+TGG + tig0024455:13907-13926 None:intergenic 25.0%
! CTTAACTAAATTCAAGCTTA+AGG - tig0024455:13908-13927 Msa1417220:CDS 25.0%
! AGATGAAATAGATTAGTACT+AGG + tig0024455:13974-13993 None:intergenic 25.0%
AAATACTATCATTTCCACCA+TGG - tig0024455:12933-12952 Msa1417220:three_prime_UTR 30.0%
! CAAAATGTTTTCAACTGGAT+TGG + tig0024455:12966-12985 None:intergenic 30.0%
! CAGAACAAAATGTTTTCAAC+TGG + tig0024455:12971-12990 None:intergenic 30.0%
! TTCTTTTTACAGTGACAGAA+GGG + tig0024455:13069-13088 None:intergenic 30.0%
! TTTCTTTTTACAGTGACAGA+AGG + tig0024455:13070-13089 None:intergenic 30.0%
CACTGTAAAAAGAAAGTTGA+TGG - tig0024455:13075-13094 Msa1417220:three_prime_UTR 30.0%
ACGAATCATCGTAAAATTGA+GGG + tig0024455:13110-13129 None:intergenic 30.0%
TTGACAATCTCTTCAACTTT+GGG + tig0024455:13135-13154 None:intergenic 30.0%
CATAAAAAACCTTAACGATG+TGG - tig0024455:13321-13340 Msa1417220:CDS 30.0%
! ATTTTCTATTTGCATCCACA+AGG + tig0024455:13379-13398 None:intergenic 30.0%
GTGATCTATGAGTTGTATAT+TGG - tig0024455:13423-13442 Msa1417220:CDS 30.0%
!! ATGACAAAAGTTGATTTTGC+AGG - tig0024455:13497-13516 Msa1417220:CDS 30.0%
!! TGACAAAAGTTGATTTTGCA+GGG - tig0024455:13498-13517 Msa1417220:CDS 30.0%
!! CTTTTGATGGTCATTGATAA+GGG + tig0024455:13746-13765 None:intergenic 30.0%
!! ACTTTTGATGGTCATTGATA+AGG + tig0024455:13747-13766 None:intergenic 30.0%
AATATTCAATCCTTTCAGAC+TGG - tig0024455:13781-13800 Msa1417220:intron 30.0%
! TGGTTTGGAATGTATTGTTA+GGG + tig0024455:13887-13906 None:intergenic 30.0%
! ATGGTTTGGAATGTATTGTT+AGG + tig0024455:13888-13907 None:intergenic 30.0%
CAAGTATGTGTTGAAAACAA+TGG + tig0024455:13997-14016 None:intergenic 30.0%
! TAGTTGTTGTCGAATTTTAC+CGG + tig0024455:14383-14402 None:intergenic 30.0%
CAACTACTACAAGAACTTAT+TGG - tig0024455:14397-14416 Msa1417220:intron 30.0%
ACAGAATTTCATCAGAACTT+AGG + tig0024455:14436-14455 None:intergenic 30.0%
CCAATGGCTTCATAAAAAAA+TGG + tig0024455:14695-14714 None:intergenic 30.0%
!!! CCATTTTTTTATGAAGCCAT+TGG - tig0024455:14692-14711 Msa1417220:CDS 30.0%
TGTGTCCTGAGTATAAAATT+TGG - tig0024455:14724-14743 Msa1417220:exon 30.0%
GTGTCCTGAGTATAAAATTT+GGG - tig0024455:14725-14744 Msa1417220:exon 30.0%
! AAAGCCCAAATTTTATACTC+AGG + tig0024455:14732-14751 None:intergenic 30.0%
! GAGTATAAAATTTGGGCTTT+TGG - tig0024455:14732-14751 Msa1417220:exon 30.0%
AAATACTATCATTTCCACCA+TGG - tig0024455:12933-12952 Msa1417220:three_prime_UTR 30.0%
! CAAAATGTTTTCAACTGGAT+TGG + tig0024455:12966-12985 None:intergenic 30.0%
! CAGAACAAAATGTTTTCAAC+TGG + tig0024455:12971-12990 None:intergenic 30.0%
! TTCTTTTTACAGTGACAGAA+GGG + tig0024455:13069-13088 None:intergenic 30.0%
! TTTCTTTTTACAGTGACAGA+AGG + tig0024455:13070-13089 None:intergenic 30.0%
CACTGTAAAAAGAAAGTTGA+TGG - tig0024455:13075-13094 Msa1417220:three_prime_UTR 30.0%
ACGAATCATCGTAAAATTGA+GGG + tig0024455:13110-13129 None:intergenic 30.0%
TTGACAATCTCTTCAACTTT+GGG + tig0024455:13135-13154 None:intergenic 30.0%
CATAAAAAACCTTAACGATG+TGG - tig0024455:13321-13340 Msa1417220:CDS 30.0%
! ATTTTCTATTTGCATCCACA+AGG + tig0024455:13379-13398 None:intergenic 30.0%
GTGATCTATGAGTTGTATAT+TGG - tig0024455:13423-13442 Msa1417220:CDS 30.0%
!! ATGACAAAAGTTGATTTTGC+AGG - tig0024455:13497-13516 Msa1417220:CDS 30.0%
!! TGACAAAAGTTGATTTTGCA+GGG - tig0024455:13498-13517 Msa1417220:CDS 30.0%
!! CTTTTGATGGTCATTGATAA+GGG + tig0024455:13746-13765 None:intergenic 30.0%
!! ACTTTTGATGGTCATTGATA+AGG + tig0024455:13747-13766 None:intergenic 30.0%
AATATTCAATCCTTTCAGAC+TGG - tig0024455:13781-13800 Msa1417220:intron 30.0%
! TGGTTTGGAATGTATTGTTA+GGG + tig0024455:13887-13906 None:intergenic 30.0%
! ATGGTTTGGAATGTATTGTT+AGG + tig0024455:13888-13907 None:intergenic 30.0%
CAAGTATGTGTTGAAAACAA+TGG + tig0024455:13997-14016 None:intergenic 30.0%
! TAGTTGTTGTCGAATTTTAC+CGG + tig0024455:14383-14402 None:intergenic 30.0%
CAACTACTACAAGAACTTAT+TGG - tig0024455:14397-14416 Msa1417220:intron 30.0%
ACAGAATTTCATCAGAACTT+AGG + tig0024455:14436-14455 None:intergenic 30.0%
CCAATGGCTTCATAAAAAAA+TGG + tig0024455:14695-14714 None:intergenic 30.0%
!!! CCATTTTTTTATGAAGCCAT+TGG - tig0024455:14692-14711 Msa1417220:CDS 30.0%
TGTGTCCTGAGTATAAAATT+TGG - tig0024455:14724-14743 Msa1417220:exon 30.0%
GTGTCCTGAGTATAAAATTT+GGG - tig0024455:14725-14744 Msa1417220:exon 30.0%
! AAAGCCCAAATTTTATACTC+AGG + tig0024455:14732-14751 None:intergenic 30.0%
! GAGTATAAAATTTGGGCTTT+TGG - tig0024455:14732-14751 Msa1417220:exon 30.0%
AGACTAGAAGAGAAAAAAGC+AGG + tig0024455:13041-13060 None:intergenic 35.0%
CACGAATCATCGTAAAATTG+AGG + tig0024455:13111-13130 None:intergenic 35.0%
CTTGACAATCTCTTCAACTT+TGG + tig0024455:13136-13155 None:intergenic 35.0%
! TTTCCATGACTGTTTTGTCA+AGG - tig0024455:13218-13237 Msa1417220:CDS 35.0%
ATACCTTGACAAAACAGTCA+TGG + tig0024455:13224-13243 None:intergenic 35.0%
! TTTTATATATAACCGCGACG+AGG - tig0024455:13291-13310 Msa1417220:CDS 35.0%
ATAACAAGTGACTGCAATTG+TGG + tig0024455:13347-13366 None:intergenic 35.0%
ATGAGTTGTATATTGGGTGT+TGG - tig0024455:13430-13449 Msa1417220:CDS 35.0%
CAAATCCTCTAGCTGAATTA+CGG + tig0024455:13590-13609 None:intergenic 35.0%
GCATTAGAGTAGAAAACAAG+AGG + tig0024455:13724-13743 None:intergenic 35.0%
AAGAGAATCTCTTCTTCCAA+AGG + tig0024455:13826-13845 None:intergenic 35.0%
TTTGGAAGAAGAGATTCTCT+TGG - tig0024455:13825-13844 Msa1417220:CDS 35.0%
! GGTTTGGAATGTATTGTTAG+GGG + tig0024455:13886-13905 None:intergenic 35.0%
AAAGACTCTACAACCAAACT+GGG - tig0024455:14249-14268 Msa1417220:intron 35.0%
!! AGTCTAGGACAAACAGATTT+TGG + tig0024455:14352-14371 None:intergenic 35.0%
AAGAACTTATTGGCCAACAA+GGG - tig0024455:14407-14426 Msa1417220:intron 35.0%
AATGTTTCCCATCTTAACCA+TGG + tig0024455:14540-14559 None:intergenic 35.0%
GGAAACATTACTCCTTTAAC+AGG - tig0024455:14551-14570 Msa1417220:CDS 35.0%
TTACTCCTTTAACAGGATCA+AGG - tig0024455:14558-14577 Msa1417220:CDS 35.0%
TACTCCTTTAACAGGATCAA+GGG - tig0024455:14559-14578 Msa1417220:CDS 35.0%
AGACTAGAAGAGAAAAAAGC+AGG + tig0024455:13041-13060 None:intergenic 35.0%
CACGAATCATCGTAAAATTG+AGG + tig0024455:13111-13130 None:intergenic 35.0%
CTTGACAATCTCTTCAACTT+TGG + tig0024455:13136-13155 None:intergenic 35.0%
! TTTCCATGACTGTTTTGTCA+AGG - tig0024455:13218-13237 Msa1417220:CDS 35.0%
ATACCTTGACAAAACAGTCA+TGG + tig0024455:13224-13243 None:intergenic 35.0%
! TTTTATATATAACCGCGACG+AGG - tig0024455:13291-13310 Msa1417220:CDS 35.0%
ATAACAAGTGACTGCAATTG+TGG + tig0024455:13347-13366 None:intergenic 35.0%
ATGAGTTGTATATTGGGTGT+TGG - tig0024455:13430-13449 Msa1417220:CDS 35.0%
CAAATCCTCTAGCTGAATTA+CGG + tig0024455:13590-13609 None:intergenic 35.0%
GCATTAGAGTAGAAAACAAG+AGG + tig0024455:13724-13743 None:intergenic 35.0%
AAGAGAATCTCTTCTTCCAA+AGG + tig0024455:13826-13845 None:intergenic 35.0%
TTTGGAAGAAGAGATTCTCT+TGG - tig0024455:13825-13844 Msa1417220:CDS 35.0%
! GGTTTGGAATGTATTGTTAG+GGG + tig0024455:13886-13905 None:intergenic 35.0%
AAAGACTCTACAACCAAACT+GGG - tig0024455:14249-14268 Msa1417220:intron 35.0%
!! AGTCTAGGACAAACAGATTT+TGG + tig0024455:14352-14371 None:intergenic 35.0%
AAGAACTTATTGGCCAACAA+GGG - tig0024455:14407-14426 Msa1417220:intron 35.0%
AATGTTTCCCATCTTAACCA+TGG + tig0024455:14540-14559 None:intergenic 35.0%
GGAAACATTACTCCTTTAAC+AGG - tig0024455:14551-14570 Msa1417220:CDS 35.0%
TTACTCCTTTAACAGGATCA+AGG - tig0024455:14558-14577 Msa1417220:CDS 35.0%
TACTCCTTTAACAGGATCAA+GGG - tig0024455:14559-14578 Msa1417220:CDS 35.0%
! ACTGGATTGGATGAATACCA+TGG + tig0024455:12953-12972 None:intergenic 40.0%
CATCGTAAAATTGAGGGTAG+AGG + tig0024455:13104-13123 None:intergenic 40.0%
ATGGAAATGTAGTCTCAGCA+AGG + tig0024455:13205-13224 None:intergenic 40.0%
GAACAATCATCAGCGAAAAG+AGG - tig0024455:13553-13572 Msa1417220:intron 40.0%
ATTCAATCCTTTCAGACTGG+TGG - tig0024455:13784-13803 Msa1417220:intron 40.0%
GTTGATCTAGTTGCTCTATC+TGG - tig0024455:13942-13961 Msa1417220:intron 40.0%
TTATGCCACTGATGCAACTA+CGG + tig0024455:14165-14184 None:intergenic 40.0%
! GTAGAGTCTTTGTCTAAAGC+TGG + tig0024455:14240-14259 None:intergenic 40.0%
CAAAGACTCTACAACCAAAC+TGG - tig0024455:14248-14267 Msa1417220:intron 40.0%
! GCAATTGAGCTGCATAGTTT+TGG + tig0024455:14301-14320 None:intergenic 40.0%
CAAGAACTTATTGGCCAACA+AGG - tig0024455:14406-14425 Msa1417220:intron 40.0%
TGAACAATTTGCCAAGTCCA+TGG - tig0024455:14520-14539 Msa1417220:CDS 40.0%
ACTCCTTTAACAGGATCAAG+GGG - tig0024455:14560-14579 Msa1417220:CDS 40.0%
! ACTGGATTGGATGAATACCA+TGG + tig0024455:12953-12972 None:intergenic 40.0%
CATCGTAAAATTGAGGGTAG+AGG + tig0024455:13104-13123 None:intergenic 40.0%
ATGGAAATGTAGTCTCAGCA+AGG + tig0024455:13205-13224 None:intergenic 40.0%
GAACAATCATCAGCGAAAAG+AGG - tig0024455:13553-13572 Msa1417220:intron 40.0%
ATTCAATCCTTTCAGACTGG+TGG - tig0024455:13784-13803 Msa1417220:intron 40.0%
GTTGATCTAGTTGCTCTATC+TGG - tig0024455:13942-13961 Msa1417220:intron 40.0%
TTATGCCACTGATGCAACTA+CGG + tig0024455:14165-14184 None:intergenic 40.0%
! GTAGAGTCTTTGTCTAAAGC+TGG + tig0024455:14240-14259 None:intergenic 40.0%
CAAAGACTCTACAACCAAAC+TGG - tig0024455:14248-14267 Msa1417220:intron 40.0%
! GCAATTGAGCTGCATAGTTT+TGG + tig0024455:14301-14320 None:intergenic 40.0%
CAAGAACTTATTGGCCAACA+AGG - tig0024455:14406-14425 Msa1417220:intron 40.0%
TGAACAATTTGCCAAGTCCA+TGG - tig0024455:14520-14539 Msa1417220:CDS 40.0%
ACTCCTTTAACAGGATCAAG+GGG - tig0024455:14560-14579 Msa1417220:CDS 40.0%
GGATTGGATGAATACCATGG+TGG + tig0024455:12950-12969 None:intergenic 45.0%
TGTCAAGTCCGTAGTTGCTA+AGG - tig0024455:13149-13168 Msa1417220:CDS 45.0%
CAATTGTGGCCACATCGTTA+AGG + tig0024455:13333-13352 None:intergenic 45.0%
! TCAGTGTTGCTAGATAGCAG+TGG - tig0024455:13531-13550 Msa1417220:intron 45.0%
CCTCTAGCTGAATTACGGTT+TGG + tig0024455:13585-13604 None:intergenic 45.0%
CCAAACCGTAATTCAGCTAG+AGG - tig0024455:13582-13601 Msa1417220:intron 45.0%
ATCAGCACAAGAGACTGTCT+GGG + tig0024455:13655-13674 None:intergenic 45.0%
TATCAGCACAAGAGACTGTC+TGG + tig0024455:13656-13675 None:intergenic 45.0%
! ACCAAGTTGGGATGTACCTT+TGG - tig0024455:13807-13826 Msa1417220:CDS 45.0%
! TCCAAAGGTACATCCCAACT+TGG + tig0024455:13811-13830 None:intergenic 45.0%
! TCTCTTGGTGCAAGCATAAG+TGG - tig0024455:13840-13859 Msa1417220:CDS 45.0%
TGGAATGTATTGTTAGGGGC+AGG + tig0024455:13882-13901 None:intergenic 45.0%
CTGCAACACAATCTCTATCG+CGG + tig0024455:14063-14082 None:intergenic 45.0%
TTCTTCCGTAGTTGCATCAG+TGG - tig0024455:14157-14176 Msa1417220:CDS 45.0%
GTTATAGGTAGCCACACCAT+AGG - tig0024455:14200-14219 Msa1417220:CDS 45.0%
CTCTACAACCAAACTGGGAA+TGG - tig0024455:14254-14273 Msa1417220:intron 45.0%
TTTGGTCTCCACCAGATCTT+GGG + tig0024455:14334-14353 None:intergenic 45.0%
! TTTTGGTCTCCACCAGATCT+TGG + tig0024455:14335-14354 None:intergenic 45.0%
TGTCCTAGACTTTGTCACAC+CGG - tig0024455:14361-14380 Msa1417220:intron 45.0%
TTACCGGTGTGACAAAGTCT+AGG + tig0024455:14367-14386 None:intergenic 45.0%
AGAACTTAGGAGACCCTTGT+TGG + tig0024455:14423-14442 None:intergenic 45.0%
TGCCAAGTCCATGGTTAAGA+TGG - tig0024455:14529-14548 Msa1417220:CDS 45.0%
GCCAAGTCCATGGTTAAGAT+GGG - tig0024455:14530-14549 Msa1417220:CDS 45.0%
TCCCATCTTAACCATGGACT+TGG + tig0024455:14534-14553 None:intergenic 45.0%
TCACCCCTTGATCCTGTTAA+AGG + tig0024455:14566-14585 None:intergenic 45.0%
GGATTGGATGAATACCATGG+TGG + tig0024455:12950-12969 None:intergenic 45.0%
TGTCAAGTCCGTAGTTGCTA+AGG - tig0024455:13149-13168 Msa1417220:CDS 45.0%
CAATTGTGGCCACATCGTTA+AGG + tig0024455:13333-13352 None:intergenic 45.0%
! TCAGTGTTGCTAGATAGCAG+TGG - tig0024455:13531-13550 Msa1417220:intron 45.0%
CCTCTAGCTGAATTACGGTT+TGG + tig0024455:13585-13604 None:intergenic 45.0%
CCAAACCGTAATTCAGCTAG+AGG - tig0024455:13582-13601 Msa1417220:intron 45.0%
ATCAGCACAAGAGACTGTCT+GGG + tig0024455:13655-13674 None:intergenic 45.0%
TATCAGCACAAGAGACTGTC+TGG + tig0024455:13656-13675 None:intergenic 45.0%
! ACCAAGTTGGGATGTACCTT+TGG - tig0024455:13807-13826 Msa1417220:CDS 45.0%
! TCCAAAGGTACATCCCAACT+TGG + tig0024455:13811-13830 None:intergenic 45.0%
! TCTCTTGGTGCAAGCATAAG+TGG - tig0024455:13840-13859 Msa1417220:CDS 45.0%
TGGAATGTATTGTTAGGGGC+AGG + tig0024455:13882-13901 None:intergenic 45.0%
CTGCAACACAATCTCTATCG+CGG + tig0024455:14063-14082 None:intergenic 45.0%
TTCTTCCGTAGTTGCATCAG+TGG - tig0024455:14157-14176 Msa1417220:CDS 45.0%
GTTATAGGTAGCCACACCAT+AGG - tig0024455:14200-14219 Msa1417220:CDS 45.0%
CTCTACAACCAAACTGGGAA+TGG - tig0024455:14254-14273 Msa1417220:intron 45.0%
TTTGGTCTCCACCAGATCTT+GGG + tig0024455:14334-14353 None:intergenic 45.0%
! TTTTGGTCTCCACCAGATCT+TGG + tig0024455:14335-14354 None:intergenic 45.0%
TGTCCTAGACTTTGTCACAC+CGG - tig0024455:14361-14380 Msa1417220:intron 45.0%
TTACCGGTGTGACAAAGTCT+AGG + tig0024455:14367-14386 None:intergenic 45.0%
AGAACTTAGGAGACCCTTGT+TGG + tig0024455:14423-14442 None:intergenic 45.0%
TGCCAAGTCCATGGTTAAGA+TGG - tig0024455:14529-14548 Msa1417220:CDS 45.0%
GCCAAGTCCATGGTTAAGAT+GGG - tig0024455:14530-14549 Msa1417220:CDS 45.0%
TCCCATCTTAACCATGGACT+TGG + tig0024455:14534-14553 None:intergenic 45.0%
TCACCCCTTGATCCTGTTAA+AGG + tig0024455:14566-14585 None:intergenic 45.0%
AGCGACAGCCTTAGCAACTA+CGG + tig0024455:13160-13179 None:intergenic 50.0%
TTATGTGTCTGACCTCGTCG+CGG + tig0024455:13306-13325 None:intergenic 50.0%
GGGACACTCTTTCTCTACAG+CGG + tig0024455:13634-13653 None:intergenic 50.0%
TCAGCACAAGAGACTGTCTG+GGG + tig0024455:13654-13673 None:intergenic 50.0%
ACTTGGTCCACCAGTCTGAA+AGG + tig0024455:13794-13813 None:intergenic 50.0%
! TTCAGACTGGTGGACCAAGT+TGG - tig0024455:13794-13813 Msa1417220:CDS 50.0%
! TCAGACTGGTGGACCAAGTT+GGG - tig0024455:13795-13814 Msa1417220:CDS 50.0%
! CTGACTTGCCATTCCCAGTT+TGG + tig0024455:14265-14284 None:intergenic 50.0%
ATGCAGCTCAATTGCGCACT+CGG - tig0024455:14306-14325 Msa1417220:intron 50.0%
AGCGACAGCCTTAGCAACTA+CGG + tig0024455:13160-13179 None:intergenic 50.0%
TTATGTGTCTGACCTCGTCG+CGG + tig0024455:13306-13325 None:intergenic 50.0%
GGGACACTCTTTCTCTACAG+CGG + tig0024455:13634-13653 None:intergenic 50.0%
TCAGCACAAGAGACTGTCTG+GGG + tig0024455:13654-13673 None:intergenic 50.0%
ACTTGGTCCACCAGTCTGAA+AGG + tig0024455:13794-13813 None:intergenic 50.0%
! TTCAGACTGGTGGACCAAGT+TGG - tig0024455:13794-13813 Msa1417220:CDS 50.0%
! TCAGACTGGTGGACCAAGTT+GGG - tig0024455:13795-13814 Msa1417220:CDS 50.0%
! CTGACTTGCCATTCCCAGTT+TGG + tig0024455:14265-14284 None:intergenic 50.0%
ATGCAGCTCAATTGCGCACT+CGG - tig0024455:14306-14325 Msa1417220:intron 50.0%
TGTCGCTAAAGAACCCCGCA+TGG - tig0024455:13173-13192 Msa1417220:CDS 55.0%
TCAGCAAGGAAGCAGCCATG+CGG + tig0024455:13191-13210 None:intergenic 55.0%
GCCACACCATAGGAGATTCG+CGG - tig0024455:14210-14229 Msa1417220:CDS 55.0%
ACCGCGAATCTCCTATGGTG+TGG + tig0024455:14214-14233 None:intergenic 55.0%
GGACACAGCGACTATGCCAA+TGG + tig0024455:14711-14730 None:intergenic 55.0%
TGTCGCTAAAGAACCCCGCA+TGG - tig0024455:13173-13192 Msa1417220:CDS 55.0%
TCAGCAAGGAAGCAGCCATG+CGG + tig0024455:13191-13210 None:intergenic 55.0%
GCCACACCATAGGAGATTCG+CGG - tig0024455:14210-14229 Msa1417220:CDS 55.0%
ACCGCGAATCTCCTATGGTG+TGG + tig0024455:14214-14233 None:intergenic 55.0%
GGACACAGCGACTATGCCAA+TGG + tig0024455:14711-14730 None:intergenic 55.0%
AGCAAGGAAGCAGCCATGCG+GGG + tig0024455:13189-13208 None:intergenic 60.0%
CAGCAAGGAAGCAGCCATGC+GGG + tig0024455:13190-13209 None:intergenic 60.0%
GGTGCACCGCGAATCTCCTA+TGG + tig0024455:14219-14238 None:intergenic 60.0%
!! ACTCGGTGCCCAAGATCTGG+TGG - tig0024455:14323-14342 Msa1417220:intron 60.0%
AGCAAGGAAGCAGCCATGCG+GGG + tig0024455:13189-13208 None:intergenic 60.0%
CAGCAAGGAAGCAGCCATGC+GGG + tig0024455:13190-13209 None:intergenic 60.0%
GGTGCACCGCGAATCTCCTA+TGG + tig0024455:14219-14238 None:intergenic 60.0%
!! ACTCGGTGCCCAAGATCTGG+TGG - tig0024455:14323-14342 Msa1417220:intron 60.0%
!! CGCACTCGGTGCCCAAGATC+TGG - tig0024455:14320-14339 Msa1417220:intron 65.0%
!! CGCACTCGGTGCCCAAGATC+TGG - tig0024455:14320-14339 Msa1417220:intron 65.0%
Chromosome Type Strat End Strand Name
tig0024455 gene 12900 14856 12900 ID=Msa1417220;Name=Msa1417220
tig0024455 mRNA 12900 14856 12900 ID=Msa1417220-mRNA-1;Parent=Msa1417220;Name=Msa1417220-mRNA-1;_AED=0.07;_eAED=0.07;_QI=115|0.66|0.75|1|1|1|4|237|336
tig0024455 exon 12900 13549 12900 ID=Msa1417220-mRNA-1:exon:23301;Parent=Msa1417220-mRNA-1
tig0024455 exon 13793 13957 13793 ID=Msa1417220-mRNA-1:exon:23300;Parent=Msa1417220-mRNA-1
tig0024455 exon 14030 14237 14030 ID=Msa1417220-mRNA-1:exon:23299;Parent=Msa1417220-mRNA-1
tig0024455 exon 14517 14856 14517 ID=Msa1417220-mRNA-1:exon:23298;Parent=Msa1417220-mRNA-1
tig0024455 five_prime_UTR 14742 14856 14742 ID=Msa1417220-mRNA-1:five_prime_utr;Parent=Msa1417220-mRNA-1
tig0024455 CDS 14517 14741 14517 ID=Msa1417220-mRNA-1:cds;Parent=Msa1417220-mRNA-1
tig0024455 CDS 14030 14237 14030 ID=Msa1417220-mRNA-1:cds;Parent=Msa1417220-mRNA-1
tig0024455 CDS 13793 13957 13793 ID=Msa1417220-mRNA-1:cds;Parent=Msa1417220-mRNA-1
tig0024455 CDS 13137 13549 13137 ID=Msa1417220-mRNA-1:cds;Parent=Msa1417220-mRNA-1
tig0024455 three_prime_UTR 12900 13136 12900 ID=Msa1417220-mRNA-1:three_prime_utr;Parent=Msa1417220-mRNA-1
Gene Sequence

>Msa1417220

ATGGTCAATTCTATAAACTTTCTCCTGCTTTTTTCTCTTCTAGTCTTTGCTCCCTTCTGTCACTGTAAAAAGAAAGTTGATGGTTACCTCTACCCTCAATTTTACGATGATTCGTGTCCCAAAGTTGAAGAGATTGTCAAGTCCGTAGTTGCTAAGGCTGTCGCTAAAGAACCCCGCATGGCTGCTTCCTTGCTGAGACTACATTTCCATGACTGTTTTGTCAAGGGTTGTGATGCATCAGTGTTGCTAGATAGCAGTGGAACAATCATCAGCGAAAAGAGGTCAAATCCAAACCGTAATTCAGCTAGAGGATTTGAAGTCATTGAAGAAATTAAATCCGCTGTAGAGAAAGAGTGTCCCCAGACAGTCTCTTGTGCTGATATTTTGACTCTAGCTGCTAGAGATTCAACTGTTCTTGTAAATATTTCCTCTTACTGGTGGACCAAGTTGGGATGTACCTTTGGAAGAAGAGATTCTCTTGGTGCAAGCATAAGTGGCTCAAACAACAACATTCCTGCCCCTAACAATACATTCCAAACCATCTTAACTAAATTCAAGCTTAAGGGACTTAACATTGTTGATCTAGTTGCTCTATCTGGTAGCCACACCATAGGAGATTCGCGGTGCACCAGCTTTAGACAAAGACTCTACAACCAAACTGGGAATGGCAAGTCAGATTTCACTCTTGACCAAAACTATGCAGCTCAATTGCGCACTCGGTGCCCAAGATCTGGTGGAGACCAAAATCTGTTTGTCCTAGACTTTGTCACACCGGTAAAATTCGACAACAACTACTACAAGAACTTATTGGCCAACAAGGGTCTCCTAAGTTCTGATGAAATTCTGTTGACAAAGAATCAAGTATCTGCAGATTTAGTGAAAAAGTATGCAGAAAGCAATGATCTTTTCTTTGAACAATTTGCCAAGTCCATGGTTAAGATGGGAAACATTACTCCTTTAACAGGATCAAGGGGTGAAATCAGAAAACGTTGCAGAAAGATTAACAACTGA
Protein sequence

>Msa1417220

MVNSINFLLLFSLLVFAPFCHCKKKVDGYLYPQFYDDSCPKVEEIVKSVVAKAVAKEPRMAASLLRLHFHDCFVKGCDASVLLDSSGTIISEKRSNPNRNSARGFEVIEEIKSAVEKECPQTVSCADILTLAARDSTVLVNISSYWWTKLGCTFGRRDSLGASISGSNNNIPAPNNTFQTILTKFKLKGLNIVDLVALSGSHTIGDSRCTSFRQRLYNQTGNGKSDFTLDQNYAAQLRTRCPRSGGDQNLFVLDFVTPVKFDNNYYKNLLANKGLLSSDEILLTKNQVSADLVKKYAESNDLFFEQFAKSMVKMGNITPLTGSRGEIRKRCRKINN*