AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1320070

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1320070 MtrunA17_Chr8g0351091 97.044 609 10 2 69 670 5 612 0.0 1228
Msa1320070 MtrunA17_Chr7g0246371 81.848 606 99 3 72 670 63 664 0.0 1018
Msa1320070 MtrunA17_Chr3g0138381 56.571 624 241 6 65 668 50 663 0.0 712
Msa1320070 MtrunA17_Chr2g0290961 53.675 585 252 5 95 669 91 666 0.0 634
Msa1320070 MtrunA17_Chr8g0383241 56.612 484 193 3 197 670 15 491 0.0 581
Msa1320070 MtrunA17_Chr2g0283461 48.085 470 224 6 214 669 70 533 8.50e-163 478
Msa1320070 MtrunA17_Chr7g0235161 56.934 411 171 4 261 668 151 558 5.78e-158 467
Msa1320070 MtrunA17_Chr4g0061711 47.007 451 223 4 226 668 92 534 4.20e-150 446
Msa1320070 MtrunA17_Chr2g0308241 46.652 448 229 4 226 668 92 534 8.46e-149 442
Msa1320070 MtrunA17_Chr6g0456071 51.190 420 198 5 254 668 136 553 1.09e-146 437
Msa1320070 MtrunA17_Chr7g0260711 45.161 465 218 4 215 669 216 653 6.38e-145 436
Msa1320070 MtrunA17_Chr2g0277591 43.312 471 221 8 214 669 70 509 1.50e-133 402
Msa1320070 MtrunA17_Chr1g0184751 43.432 472 224 8 208 668 158 597 1.60e-132 402
Msa1320070 MtrunA17_Chr7g0218571 41.845 466 235 10 227 670 86 537 5.09e-123 376
Msa1320070 MtrunA17_Chr2g0319311 41.106 416 228 5 270 668 124 539 9.49e-114 352
Msa1320070 MtrunA17_Chr6g0454101 44.019 418 215 7 271 670 116 532 6.16e-111 345
Msa1320070 MtrunA17_Chr4g0076011 39.130 414 226 5 281 668 58 471 3.83e-104 325
Msa1320070 MtrunA17_Chr3g0097091 38.653 401 222 7 285 668 135 528 1.32e-93 299
Msa1320070 MtrunA17_Chr7g0245621 31.612 484 292 7 201 668 183 643 3.70e-74 251
Msa1320070 MtrunA17_Chr4g0076331 57.000 200 56 3 473 669 1 173 9.61e-68 219
Msa1320070 MtrunA17_Chr8g0351141 88.406 69 7 1 1 68 83 151 3.03e-33 130
Msa1320070 MtrunA17_Chr8g0351121 79.104 67 14 0 2 68 118 184 1.32e-26 112
Msa1320070 MtrunA17_Chr6g0451981 26.627 338 199 12 345 663 64 371 5.68e-24 104
Msa1320070 MtrunA17_Chr7g0259321 32.000 175 114 4 474 643 151 325 1.12e-21 97.1
Msa1320070 MtrunA17_Chr5g0414981 27.928 333 190 15 341 658 76 373 5.17e-21 96.7
Msa1320070 MtrunA17_Chr4g0066331 27.413 259 157 8 416 643 62 320 6.87e-21 94.7
Msa1320070 MtrunA17_Chr3g0127611 30.612 196 128 5 474 661 140 335 7.07e-21 94.7
Msa1320070 MtrunA17_Chr7g0269751 27.358 318 181 11 355 660 74 353 8.15e-21 94.7
Msa1320070 MtrunA17_Chr8g0351111 63.768 69 24 1 1 68 80 148 1.17e-20 93.6
Msa1320070 MtrunA17_Chr3g0141321 28.497 193 125 6 474 655 139 329 3.83e-19 89.4
Msa1320070 MtrunA17_Chr8g0373671 27.751 209 134 6 442 640 115 316 5.15e-18 86.3
Msa1320070 MtrunA17_Chr1g0154521 28.866 194 124 6 474 655 165 356 4.48e-17 83.6
Msa1320070 MtrunA17_Chr7g0246361 58.209 67 25 1 1 67 61 124 1.05e-16 81.3
Msa1320070 MtrunA17_Chr1g0196511 31.429 175 115 3 474 643 154 328 2.13e-16 81.3
Msa1320070 MtrunA17_Chr5g0403501 29.480 173 116 4 474 640 166 338 8.87e-16 79.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1320070 AT3G61130.1 71.028 642 171 5 40 670 36 673 0.0 947
Msa1320070 AT4G38270.2 54.397 614 249 6 83 670 68 676 0.0 702
Msa1320070 AT4G38270.1 60.189 530 200 2 152 670 151 680 0.0 697
Msa1320070 AT2G46480.3 59.057 530 205 4 149 670 15 540 0.0 653
Msa1320070 AT2G46480.4 59.057 530 205 4 149 670 28 553 0.0 653
Msa1320070 AT5G47780.1 62.174 460 163 2 216 668 159 614 0.0 627
Msa1320070 AT2G46480.2 55.660 530 198 5 149 670 15 515 0.0 598
Msa1320070 AT2G46480.1 55.660 530 198 5 149 670 28 528 0.0 597
Msa1320070 AT3G25140.1 56.161 422 176 5 253 668 139 557 4.10e-170 498
Msa1320070 AT1G18580.1 48.330 449 218 5 226 666 92 534 5.58e-156 461
Msa1320070 AT3G02350.1 51.716 437 203 5 238 668 125 559 3.70e-149 444
Msa1320070 AT2G20810.1 48.230 452 221 6 226 669 90 536 8.26e-148 440
Msa1320070 AT2G30575.1 43.348 466 228 4 215 670 171 610 1.40e-136 414
Msa1320070 AT1G06780.1 43.656 465 227 3 215 669 149 588 3.26e-135 409
Msa1320070 AT1G06780.2 43.656 465 227 3 215 669 162 601 1.34e-134 408
Msa1320070 AT3G01040.2 44.498 418 215 5 270 670 115 532 7.29e-118 363
Msa1320070 AT3G01040.3 44.498 418 215 5 270 670 115 532 7.29e-118 363
Msa1320070 AT5G15470.1 45.084 417 212 5 271 670 116 532 2.13e-117 362
Msa1320070 AT5G54690.1 39.252 428 236 6 265 668 105 532 3.31e-111 346
Msa1320070 AT3G01040.1 41.627 418 194 6 270 670 115 499 1.69e-102 322
Msa1320070 AT3G58790.2 37.931 406 224 8 285 668 7 406 1.32e-88 283
Msa1320070 AT3G58790.1 36.385 426 243 8 265 668 119 538 1.43e-87 284
Msa1320070 AT2G38650.2 33.954 483 279 9 201 669 162 618 2.94e-87 286
Msa1320070 AT2G38650.1 33.954 483 279 9 201 669 162 618 2.94e-87 286
Msa1320070 AT2G38650.3 34.447 389 216 8 201 576 162 524 1.20e-65 226
Msa1320070 AT1G02720.1 27.900 319 185 11 355 662 77 361 2.20e-22 99.8
Msa1320070 AT1G02720.2 27.900 319 185 11 355 662 77 361 2.20e-22 99.8
Msa1320070 AT4G02130.3 26.543 324 193 10 350 662 57 346 5.92e-22 98.2
Msa1320070 AT4G02130.1 26.543 324 193 10 350 662 57 346 5.92e-22 98.2
Msa1320070 AT4G02130.2 26.543 324 193 10 350 662 57 346 5.92e-22 98.2
Msa1320070 AT3G06260.1 30.857 175 116 3 474 643 150 324 6.72e-22 98.2
Msa1320070 AT3G62660.1 26.498 317 185 10 355 660 79 358 1.20e-21 97.8
Msa1320070 AT1G70090.1 26.349 315 191 11 357 660 80 364 3.52e-21 96.7
Msa1320070 AT1G70090.2 26.349 315 191 11 357 660 80 364 3.52e-21 96.7
Msa1320070 AT3G28340.1 29.536 237 150 9 440 664 126 357 1.14e-20 94.7
Msa1320070 AT2G45630.1 61.765 68 26 0 1 68 95 162 1.02e-19 87.8
Msa1320070 AT2G45630.2 46.789 109 54 1 1 109 95 199 7.96e-19 89.0
Msa1320070 AT1G19300.1 30.857 175 111 5 474 640 150 322 8.07e-19 89.0
Msa1320070 AT1G24170.1 24.606 317 198 10 355 660 81 367 2.57e-18 88.2
Msa1320070 AT3G50760.1 26.633 199 133 5 472 659 134 330 1.08e-15 79.3
Msa1320070 AT1G13250.1 25.926 324 197 11 350 663 51 341 1.78e-15 79.0
Msa1320070 AT1G12550.1 58.929 56 23 0 1 56 79 134 2.04e-14 75.5

Find 165 sgRNAs with CRISPR-Local

Find 284 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCCATTTCTTTCATCTTTC+TGG -0.058023 8_4:-24266187 Msa1320070:three_prime_UTR
GTTACTGATAAACTCAATTT+TGG 0.156608 8_4:-24267164 Msa1320070:CDS
TTGAGCATCGAGAGATATTT+TGG 0.169108 8_4:+24266939 None:intergenic
GCATTGACTCTGTTCCTTCT+AGG 0.177965 8_4:+24266116 None:intergenic
CCAGGACTAATGACATTCTA+TGG 0.236359 8_4:-24266456 Msa1320070:CDS
AGAAGGATTTGACAGGATTA+TGG 0.240515 8_4:-24266839 Msa1320070:CDS
TGTATCCAGCACAGATGATA+TGG 0.277199 8_4:-24269319 Msa1320070:CDS
TTCGGAGGATGTTGCTGATA+TGG 0.291825 8_4:-24270607 Msa1320070:CDS
GTGTCGGCGTCGGGGAATTC+AGG 0.311339 8_4:-24270652 Msa1320070:CDS
CGATGTCATCATCAAGAAAA+AGG 0.314739 8_4:+24266868 None:intergenic
TGACAAAGTATCGGCCTTAC+TGG 0.316170 8_4:-24266299 Msa1320070:CDS
TCGTGGCATGTCCTTGGTTT+GGG 0.323379 8_4:-24266411 Msa1320070:CDS
TCTAATTTAGGATAAACTTC+TGG 0.323521 8_4:+24266897 None:intergenic
CCTCGACGTGTGTCGGCGTC+GGG 0.328540 8_4:-24270661 Msa1320070:CDS
GCTTCAGCCGCCTGCATCTT+GGG 0.330328 8_4:-24270523 Msa1320070:CDS
ATATCACAAATGGCAAAATA+TGG 0.336591 8_4:-24266625 Msa1320070:intron
ATTATGGGAAGTTGATCTTC+AGG 0.339063 8_4:-24266823 Msa1320070:CDS
ATCGTGGCATGTCCTTGGTT+TGG 0.342505 8_4:-24266412 Msa1320070:CDS
GATCTTCAGGGGAAAGTAAA+TGG 0.342595 8_4:-24266810 Msa1320070:CDS
TTCTTAACCTTGTTTCAAAT+AGG 0.344791 8_4:+24266011 None:intergenic
ATCTGTTGAGTCAGCCGTAC+TGG 0.356409 8_4:-24268057 Msa1320070:CDS
TGATATGGCTGTGGCTTTGT+TGG 0.364353 8_4:-24270592 Msa1320070:CDS
AAAGAGAGTCAACATGCTTT+AGG 0.370001 8_4:-24267879 Msa1320070:CDS
ATTCTTCTGTTCGGTTTATG+AGG 0.374399 8_4:-24267989 Msa1320070:CDS
GTGAAGGAAGGAAGATCTTC+AGG 0.374727 8_4:-24269049 Msa1320070:CDS
TATGAGGGACCAGATCATTA+TGG 0.376231 8_4:-24267973 Msa1320070:CDS
GAAGGATTTGACAGGATTAT+GGG 0.385355 8_4:-24266838 Msa1320070:CDS
TCTTGTGTAACATGTGTTGT+TGG 0.388574 8_4:+24269082 None:intergenic
AATGAAAGAGTATTATTTCA+AGG 0.389390 8_4:-24267015 Msa1320070:CDS
TACTGGGTAAATACAGCATT+TGG 0.389876 8_4:-24268040 Msa1320070:CDS
GGCTTCAGCCGCCTGCATCT+TGG 0.390288 8_4:-24270524 Msa1320070:CDS
GACAGGGTGCTTTGGAAGCT+GGG 0.390513 8_4:-24266489 Msa1320070:CDS
CCCGTGCCTTTGACAGAACT+TGG 0.391358 8_4:+24267712 None:intergenic
TAGTACACGTCAAGAAATAA+AGG 0.392316 8_4:-24267742 Msa1320070:CDS
AGACAGCTGATGAGAAAGTT+AGG 0.401919 8_4:-24267638 Msa1320070:CDS
ACATGCCACGATTTATTTAG+TGG 0.403063 8_4:+24266423 None:intergenic
GATTCAAGCTGCCGCAATAC+AGG 0.410074 8_4:+24267044 None:intergenic
AATGCTTGTGGTTGGGCATA+TGG 0.411331 8_4:-24266699 Msa1320070:CDS
TCAAAGTTTCTTGCAATATG+TGG 0.412409 8_4:+24266726 None:intergenic
TATGCCCAACCACAAGCATT+TGG 0.412414 8_4:+24266702 None:intergenic
AGTTAAGATATTTGTCAAAT+CGG 0.415942 8_4:+24266757 None:intergenic
GTTCCCTAGGAGTGAGAACT+TGG 0.416886 8_4:-24267493 Msa1320070:CDS
GGAATTCAGGTCGCCAATGC+TGG 0.417890 8_4:-24270639 Msa1320070:CDS
GAGGTCAATGTGATTAGTTC+CGG 0.424802 8_4:+24270680 None:intergenic
TGCCAGAAAGATGAAAGAAA+TGG 0.425310 8_4:+24266185 None:intergenic
GCCAGAAAGATGAAAGAAAT+GGG 0.426450 8_4:+24266186 None:intergenic
GTGCAGAGGGTCTTACTCAC+TGG 0.437169 8_4:-24268958 Msa1320070:intron
AGACAGGGTGCTTTGGAAGC+TGG 0.437302 8_4:-24266490 Msa1320070:CDS
CAAAAGAAACCACATGTTCA+TGG 0.440335 8_4:+24267139 None:intergenic
AATTCCACTTGGTATGGTCT+TGG 0.447689 8_4:+24267575 None:intergenic
TGGGTTTCTATATTTCATAT+TGG 0.450004 8_4:+24266959 None:intergenic
TGGTCAGTCTTGAACCTAGA+AGG 0.460339 8_4:-24266130 Msa1320070:three_prime_UTR
AGATGGAAAACATGCTTTGA+AGG 0.460806 8_4:+24267188 None:intergenic
GACTTCAAATGCTGCCTATG+AGG 0.462603 8_4:-24269232 Msa1320070:intron
AAAATGCTGATTCTTCTGTT+CGG 0.464420 8_4:-24267998 Msa1320070:CDS
TTAACATATTTAGTCCAGTA+AGG 0.464878 8_4:+24266285 None:intergenic
TAACTTGCATTTCTGCAGAT+AGG 0.469528 8_4:+24266251 None:intergenic
CCAAGTTCTGTCAAAGGCAC+GGG 0.472286 8_4:-24267712 Msa1320070:CDS
AATAAATCGTGGCATGTCCT+TGG 0.472321 8_4:-24266417 Msa1320070:CDS
AAAGAGTATTATTTCAAGGC+AGG 0.473099 8_4:-24267011 Msa1320070:CDS
ACCTCGACGTGTGTCGGCGT+CGG 0.475926 8_4:-24270662 Msa1320070:CDS
AGAATGAAGACAGGGTGCTT+TGG 0.476034 8_4:-24266497 Msa1320070:CDS
TGAGTTTATCAGTAACAAGA+TGG 0.477741 8_4:+24267171 None:intergenic
ACGTCAAGAAATAAAGGCTA+TGG 0.478191 8_4:-24267736 Msa1320070:CDS
CTAAAATTGTCAAGACTCAA+AGG 0.478518 8_4:+24269294 None:intergenic
CACATTAGTCCAAACCTCAT+AGG 0.483893 8_4:+24269218 None:intergenic
TGCTGCTGAGTTGGTAAAGA+TGG 0.489018 8_4:-24268129 Msa1320070:CDS
TGATCCAAATGCTTGTGGTT+GGG 0.490330 8_4:-24266706 Msa1320070:CDS
TGTTGCAAGAGCTGCAGTCT+AGG 0.490652 8_4:-24267905 Msa1320070:CDS
CTGATTCAGATCTTCATCAC+AGG 0.491823 8_4:-24267845 Msa1320070:intron
TATTGGAAGCACTAGAAGAA+AGG 0.491924 8_4:+24266976 None:intergenic
GCACTAGAAGAAAGGGTGGT+TGG 0.493295 8_4:+24266984 None:intergenic
TAGTGCATAATGATAAAGAC+TGG 0.499652 8_4:+24267464 None:intergenic
AGGCCCCATATCATCTGTGC+TGG 0.501711 8_4:+24269314 None:intergenic
TCTGCGTCATGGAGAGTTGT+TGG 0.501808 8_4:-24269261 Msa1320070:CDS
CCCTTCGGACTTGTCGTGTA+GGG 0.504060 8_4:+24268927 None:intergenic
GTGGCTTTGTTGGTTGATGT+TGG 0.505017 8_4:-24270582 Msa1320070:CDS
TATGACAGCAAGTTGGTCAC+TGG 0.506011 8_4:-24267681 Msa1320070:CDS
ATTGGAAGCACTAGAAGAAA+GGG 0.507070 8_4:+24266977 None:intergenic
ATACCCTAGCCATAATGATC+TGG 0.507931 8_4:+24267964 None:intergenic
TTCTCCAAGTTCTCACTCCT+AGG 0.508939 8_4:+24267489 None:intergenic
TTCATCATGACCTCCAGTGC+CGG 0.509827 8_4:-24270699 None:intergenic
TTATGGGAAGTTGATCTTCA+GGG 0.517146 8_4:-24266822 Msa1320070:CDS
GTATCCAGCACAGATGATAT+GGG 0.517429 8_4:-24269318 Msa1320070:CDS
TTCTTCTGTTCGGTTTATGA+GGG 0.518856 8_4:-24267988 Msa1320070:CDS
TGATGACATCGTTGTTCAGA+AGG 0.518905 8_4:-24266856 Msa1320070:CDS
GGAAGATTTCAGCGGCGGAT+CGG 0.519872 8_4:-24270557 Msa1320070:CDS
AATGTGATTAGTTCCGGCAC+TGG 0.522357 8_4:+24270686 None:intergenic
GTTGTTCAGAAGGATTTGAC+AGG 0.522397 8_4:-24266846 Msa1320070:CDS
TCACTGGCATATATCACAAA+TGG 0.525538 8_4:-24266635 Msa1320070:CDS
TTCAGCTGCTGCAAATAGAC+AGG 0.526888 8_4:-24269599 Msa1320070:intron
GCAATGAATTCCACTTGGTA+TGG 0.527610 8_4:+24267569 None:intergenic
GGAAAAGCGTGCTGCTGAGT+TGG 0.528593 8_4:-24268138 Msa1320070:CDS
CCATAGAATGTCATTAGTCC+TGG 0.529892 8_4:+24266456 None:intergenic
ATCATTGATCGATCTACAGT+TGG 0.532779 8_4:+24266381 None:intergenic
CTTTGTTGGTTGATGTTGGT+AGG 0.535021 8_4:-24270578 Msa1320070:CDS
TTGATCCAAATGCTTGTGGT+TGG 0.535817 8_4:-24266707 Msa1320070:CDS
TTCTGATCTGATGTTCAGAC+AGG 0.545504 8_4:-24265947 Msa1320070:three_prime_UTR
CATGAACGTATTTGATCTCA+AGG 0.549062 8_4:-24266676 Msa1320070:CDS
TAGAAGAAAGGGTGGTTGGA+TGG 0.549689 8_4:+24266988 None:intergenic
GCCAAGTTCTGTCAAAGGCA+CGG 0.552166 8_4:-24267713 Msa1320070:CDS
AGTGGAAAATCCCAAGATGC+AGG 0.552755 8_4:+24270513 None:intergenic
TCTGTTGAGTCAGCCGTACT+GGG 0.556493 8_4:-24268056 Msa1320070:CDS
CTAGTGTCTTGCTAGAATGA+TGG 0.558306 8_4:-24266150 Msa1320070:three_prime_UTR
CTGCCGCAATACAGGGCAGT+AGG 0.560064 8_4:+24267052 None:intergenic
CATCCTACTGCCCTGTATTG+CGG 0.562221 8_4:-24267055 Msa1320070:CDS
AACTTTGATCCAAATGCTTG+TGG 0.562703 8_4:-24266711 Msa1320070:CDS
ACCCTTCGGACTTGTCGTGT+AGG 0.562800 8_4:+24268926 None:intergenic
GGACCAGATCATTATGGCTA+GGG 0.565156 8_4:-24267967 Msa1320070:intron
CAGGACTAATGACATTCTAT+GGG 0.566407 8_4:-24266455 Msa1320070:CDS
GGGACCAGATCATTATGGCT+AGG 0.569661 8_4:-24267968 Msa1320070:CDS
CAAGTTCTGTCAAAGGCACG+GGG 0.571586 8_4:-24267711 Msa1320070:CDS
CTGGAAGATAGAATCTAAGA+TGG 0.575970 8_4:+24266916 None:intergenic
AATGTGGAAAGGAATGATGT+TGG 0.577511 8_4:+24266076 None:intergenic
TGGGTAAATACAGCATTTGG+AGG 0.579641 8_4:-24268037 Msa1320070:CDS
GTGATTAGTTCCGGCACTGG+AGG 0.582358 8_4:+24270689 None:intergenic
ATGCAAGTTACATGAATAGA+CGG 0.582875 8_4:-24266238 Msa1320070:exon
AATGCTGTATTTACCCAGTA+CGG 0.587071 8_4:+24268043 None:intergenic
GACTCATATCAGTAAAATCA+CGG 0.588030 8_4:-24265828 Msa1320070:three_prime_UTR
CGTCAAGAAATAAAGGCTAT+GGG 0.590218 8_4:-24267735 Msa1320070:CDS
GGATGTTGCTGATATGGCTG+TGG 0.591447 8_4:-24270601 Msa1320070:CDS
AAGCACATTCCTCAGTCAGT+TGG 0.593627 8_4:-24267601 Msa1320070:CDS
TATGGGAAGTTGATCTTCAG+GGG 0.594151 8_4:-24266821 Msa1320070:CDS
CTCAGCTGTTACAAATGCAA+AGG 0.595673 8_4:-24267403 Msa1320070:intron
ACATTGACCTCGACGTGTGT+CGG 0.597975 8_4:-24270668 Msa1320070:CDS
AAGCAAGAATCAATGTGGAA+AGG 0.599134 8_4:+24266065 None:intergenic
ATTCAAGCTGCCGCAATACA+GGG 0.605492 8_4:+24267045 None:intergenic
CGATTGCCATGACAAAGTAT+CGG 0.606224 8_4:-24266308 Msa1320070:CDS
TCTCCAAGTTCTCACTCCTA+GGG 0.606744 8_4:+24267490 None:intergenic
GCCCTACACGACAAGTCCGA+AGG 0.608010 8_4:-24268928 Msa1320070:CDS
GATCAGAATATTGTACAATG+AGG 0.611184 8_4:+24265962 None:intergenic
ATTCTCACCTATTTGAAACA+AGG 0.613661 8_4:-24266018 Msa1320070:three_prime_UTR
AAGCTGGGGACATTACCTCC+AGG 0.613770 8_4:-24266474 Msa1320070:CDS
GTCATGGCAATCGCTAGCCA+CGG 0.617762 8_4:+24266318 None:intergenic
TATGGGCCAAGTTCTGTCAA+AGG 0.623145 8_4:-24267718 Msa1320070:CDS
CCAGAAAGATGAAAGAAATG+GGG 0.626206 8_4:+24266187 None:intergenic
ACAGGGTGCTTTGGAAGCTG+GGG 0.627413 8_4:-24266488 Msa1320070:CDS
GCAATTGTATGACAGCAAGT+TGG 0.629238 8_4:-24267688 Msa1320070:CDS
CTCGACGTGTGTCGGCGTCG+GGG 0.632956 8_4:-24270660 Msa1320070:CDS
TCTTGGCAGCCAACTGACTG+AGG 0.635206 8_4:+24267592 None:intergenic
TGGTAGGAAGATTTCAGCGG+CGG 0.635656 8_4:-24270562 Msa1320070:CDS
CCCTACACGACAAGTCCGAA+GGG 0.638020 8_4:-24268927 Msa1320070:intron
CACATCCACTAAATAAATCG+TGG 0.639212 8_4:-24266428 Msa1320070:CDS
ACGTATTTGATCTCAAGGAG+TGG 0.640381 8_4:-24266671 Msa1320070:CDS
GATAAGCAATGAATTCCACT+TGG 0.641239 8_4:+24267564 None:intergenic
GTGGAAAGGAATGATGTTGG+AGG 0.644209 8_4:+24266079 None:intergenic
AGTGCATAATGATAAAGACT+GGG 0.649951 8_4:+24267465 None:intergenic
GCAGCAGTTGTACATTACAA+TGG 0.655408 8_4:-24266351 Msa1320070:CDS
GTAAGGCCGATACTTTGTCA+TGG 0.657953 8_4:+24266302 None:intergenic
TAGAATGTCATTAGTCCTGG+AGG 0.659704 8_4:+24266459 None:intergenic
GGAGGTAGTAATCTATTGTG+AGG 0.664103 8_4:+24267535 None:intergenic
ATTCAAAGCAAGAATCAATG+TGG 0.665211 8_4:+24266060 None:intergenic
TATCCAGCACAGATGATATG+GGG 0.668316 8_4:-24269317 Msa1320070:CDS
CAGAAAGATGAAAGAAATGG+GGG 0.671244 8_4:+24266188 None:intergenic
GGAAGCACTAGAAGAAAGGG+TGG 0.674642 8_4:+24266980 None:intergenic
TGTTGGTAGGAAGATTTCAG+CGG 0.675352 8_4:-24270565 Msa1320070:CDS
GGAAAATCCCAAGATGCAGG+CGG 0.680887 8_4:+24270516 None:intergenic
AACAGCTGAGTTCACAACAA+CGG 0.690951 8_4:+24267416 None:intergenic
ACAATGGTAATATGAAGCCG+TGG 0.696694 8_4:-24266335 Msa1320070:CDS
TTGGACTATAACCCAAACCA+AGG 0.706567 8_4:+24266400 None:intergenic
CCCGACGCCGACACACGTCG+AGG 0.714708 8_4:+24270661 None:intergenic
AAGGAAGGAAGATCTTCAGG+TGG 0.717716 8_4:-24269046 Msa1320070:intron
GCTGCCAAGACCATACCAAG+TGG 0.721158 8_4:-24267579 Msa1320070:CDS
AATGGTGCAGTTGAAACATG+TGG 0.724609 8_4:-24266792 Msa1320070:CDS
AAGTTCTGTCAAAGGCACGG+GGG 0.753594 8_4:-24267710 Msa1320070:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTATACTTATTTTAAATAC+AGG + chr8_4:24267351-24267370 None:intergenic 10.0%
!!! TTTAAATTTTCTAAGATTTA+AGG + chr8_4:24267983-24268002 None:intergenic 10.0%
!! TTAAAGTAGATTATCAAAAA+TGG + chr8_4:24266480-24266499 None:intergenic 15.0%
!!! ATGGTTGATTTTTTAATTAA+AGG - chr8_4:24267408-24267427 Msa1320070:CDS 15.0%
!!! ATATTTTCTCAAATCATATA+TGG + chr8_4:24269256-24269275 None:intergenic 15.0%
!!! AAAAGGATTTTATCTAATTT+AGG + chr8_4:24269639-24269658 None:intergenic 15.0%
!! ATTAATGATAAACTGAATGA+TGG - chr8_4:24266315-24266334 Msa1320070:CDS 20.0%
!! GTAGTGTTTGATAAAATTAA+CGG - chr8_4:24266362-24266381 Msa1320070:CDS 20.0%
!!! GTATATTTATTAGTTTAAGC+CGG - chr8_4:24266859-24266878 Msa1320070:CDS 20.0%
!!! TTTATTTTTGACATCAACAA+AGG - chr8_4:24267721-24267740 Msa1320070:CDS 20.0%
!! AATTGTAAAATCAAATAGTG+AGG + chr8_4:24267824-24267843 None:intergenic 20.0%
!! AAATATTTCAGAAAGTAAAC+AGG - chr8_4:24268008-24268027 Msa1320070:CDS 20.0%
!! AATTATTCACTGTCTATAAA+AGG + chr8_4:24269192-24269211 None:intergenic 20.0%
!!! AAAATGTAGATGATTTTAAG+TGG - chr8_4:24269436-24269455 Msa1320070:intron 20.0%
!!! AATGAAAGAGTATTATTTCA+AGG - chr8_4:24269506-24269525 Msa1320070:intron 20.0%
!! AGTTAAGATATTTGTCAAAT+CGG + chr8_4:24269767-24269786 None:intergenic 20.0%
!! AAATCAGATTAAAAAAAGCT+AGG + chr8_4:24270003-24270022 None:intergenic 20.0%
!!! CAATGCTGGAAATATTTTTT+CGG - chr8_4:24265896-24265915 Msa1320070:three_prime_UTR 25.0%
! ACATTTGAACAACAAATACA+TGG + chr8_4:24266111-24266130 None:intergenic 25.0%
!! ATCAAAGTTGTCTTTTCAAA+TGG - chr8_4:24266269-24266288 Msa1320070:CDS 25.0%
! TCAAAAATGGCTATAAGATT+TGG + chr8_4:24266467-24266486 None:intergenic 25.0%
! CTTAGCTTATATTTATTCTC+TGG - chr8_4:24266506-24266525 Msa1320070:intron 25.0%
!!! TACAAAGTGTTTTCAAATAG+TGG - chr8_4:24266743-24266762 Msa1320070:CDS 25.0%
! TTGGCTATGTAACTAAATAA+TGG - chr8_4:24266964-24266983 Msa1320070:CDS 25.0%
! ATCAACCATGAATTAACATT+TGG + chr8_4:24267397-24267416 None:intergenic 25.0%
!!! CAATTATTTTTTTGTGCAGA+GGG - chr8_4:24267550-24267569 Msa1320070:CDS 25.0%
! ATCAACAAAGGAAAAAGTAA+GGG - chr8_4:24267733-24267752 Msa1320070:CDS 25.0%
! GAAAATGTAAACATGAAACA+CGG + chr8_4:24267760-24267779 None:intergenic 25.0%
!!! ACATTTTTTATGAGAGAAGA+CGG + chr8_4:24267938-24267957 None:intergenic 25.0%
!!! TTTTCTAAGATTTAAGGAGA+CGG + chr8_4:24267977-24267996 None:intergenic 25.0%
! CTAAAAGCTAATATCATTTG+AGG + chr8_4:24268048-24268067 None:intergenic 25.0%
!!! TAGAGCATTTTATTCTCTTT+TGG - chr8_4:24268128-24268147 Msa1320070:CDS 25.0%
!! GAGAATATTTTCAATCATGT+TGG + chr8_4:24268181-24268200 None:intergenic 25.0%
!! ACTTTGAGTGTTTTTCATTT+TGG - chr8_4:24268300-24268319 Msa1320070:intron 25.0%
!! CTTTGAGTGTTTTTCATTTT+GGG - chr8_4:24268301-24268320 Msa1320070:intron 25.0%
! TGCAGCAATTAATAGAAAAA+AGG - chr8_4:24268358-24268377 Msa1320070:intron 25.0%
! TAAGCAGAAAATTCAGAAAA+AGG + chr8_4:24268737-24268756 None:intergenic 25.0%
! ATAAAAGGAAATAATGCACA+CGG + chr8_4:24269177-24269196 None:intergenic 25.0%
!! GTTACTGATAAACTCAATTT+TGG - chr8_4:24269357-24269376 Msa1320070:intron 25.0%
!! AATCATCTACATTTTGAACA+TGG + chr8_4:24269431-24269450 None:intergenic 25.0%
! TGGGTTTCTATATTTCATAT+TGG + chr8_4:24269565-24269584 None:intergenic 25.0%
!! TCTAATTTAGGATAAACTTC+TGG + chr8_4:24269627-24269646 None:intergenic 25.0%
! ATATCACAAATGGCAAAATA+TGG - chr8_4:24269896-24269915 Msa1320070:intron 25.0%
! TTAACATATTTAGTCCAGTA+AGG + chr8_4:24270239-24270258 None:intergenic 25.0%
!! TTCTTAACCTTGTTTCAAAT+AGG + chr8_4:24270513-24270532 None:intergenic 25.0%
CGAAAAAATATTTCCAGCAT+TGG + chr8_4:24265898-24265917 None:intergenic 30.0%
CACATGATGTTAGAAAAACA+AGG - chr8_4:24266240-24266259 Msa1320070:exon 30.0%
AATTTCAAGGTCATAGAAGA+TGG + chr8_4:24266439-24266458 None:intergenic 30.0%
!! AGATTTGGTTGGTAATTTCA+AGG + chr8_4:24266452-24266471 None:intergenic 30.0%
! AAATGGCTATAAGATTTGGT+TGG + chr8_4:24266463-24266482 None:intergenic 30.0%
! CTTTCGAAAAGGTTGATTTA+AGG + chr8_4:24267081-24267100 None:intergenic 30.0%
! TAAATCAACCTTTTCGAAAG+AGG - chr8_4:24267081-24267100 Msa1320070:CDS 30.0%
CTAAAATTGTCAAGACTCAA+AGG + chr8_4:24267230-24267249 None:intergenic 30.0%
TGGAGGCAATAAAACAATAA+TGG + chr8_4:24267377-24267396 None:intergenic 30.0%
AACCATGAATTAACATTTGG+AGG + chr8_4:24267394-24267413 None:intergenic 30.0%
TGTTACACAAGAAAAACGAA+AGG - chr8_4:24267450-24267469 Msa1320070:CDS 30.0%
CCTCTGCACAAAAAAATAAT+TGG + chr8_4:24267552-24267571 None:intergenic 30.0%
!!! CCAATTATTTTTTTGTGCAG+AGG - chr8_4:24267549-24267568 Msa1320070:CDS 30.0%
TTCTTGTTAGAACTTGCATT+TGG - chr8_4:24267638-24267657 Msa1320070:CDS 30.0%
AACTATCACTAACATAAGGA+AGG + chr8_4:24267678-24267697 None:intergenic 30.0%
TGCAAACTATCACTAACATA+AGG + chr8_4:24267682-24267701 None:intergenic 30.0%
CATCAACAAAGGAAAAAGTA+AGG - chr8_4:24267732-24267751 Msa1320070:CDS 30.0%
! TGCTAAAACAGTGTTTTCAT+TGG - chr8_4:24267881-24267900 Msa1320070:CDS 30.0%
AAAGCTAATATCATTTGAGG+TGG + chr8_4:24268045-24268064 None:intergenic 30.0%
! GTTGGAGAATATTACGTTTT+AGG + chr8_4:24268163-24268182 None:intergenic 30.0%
TTCTCATTCTTACTGAATTG+TGG - chr8_4:24268196-24268215 Msa1320070:intron 30.0%
!! AGTGTTTTTCATTTTGGGAA+TGG - chr8_4:24268306-24268325 Msa1320070:intron 30.0%
!! GTGTTTTTCATTTTGGGAAT+GGG - chr8_4:24268307-24268326 Msa1320070:intron 30.0%
GCAATTAATAGAAAAAAGGC+AGG - chr8_4:24268362-24268381 Msa1320070:intron 30.0%
!! AAAATGCTGATTCTTCTGTT+CGG - chr8_4:24268523-24268542 Msa1320070:intron 30.0%
TAGTACACGTCAAGAAATAA+AGG - chr8_4:24268779-24268798 Msa1320070:intron 30.0%
AGTGCATAATGATAAAGACT+GGG + chr8_4:24269059-24269078 None:intergenic 30.0%
TAGTGCATAATGATAAAGAC+TGG + chr8_4:24269060-24269079 None:intergenic 30.0%
! ACTATTTTCAGACAATGTCT+TGG - chr8_4:24269076-24269095 Msa1320070:CDS 30.0%
!! ATAATTTGTTCCTTGACTGT+AGG - chr8_4:24269206-24269225 Msa1320070:intron 30.0%
!! TAATTTGTTCCTTGACTGTA+GGG - chr8_4:24269207-24269226 Msa1320070:intron 30.0%
AACATAAAGACAATGAGAGT+TGG + chr8_4:24269291-24269310 None:intergenic 30.0%
TGAGTTTATCAGTAACAAGA+TGG + chr8_4:24269353-24269372 None:intergenic 30.0%
!! AAAGAGTATTATTTCAAGGC+AGG - chr8_4:24269510-24269529 Msa1320070:intron 30.0%
TCAAAGTTTCTTGCAATATG+TGG + chr8_4:24269798-24269817 None:intergenic 30.0%
!!! TCTGATTTTAGAATGAAGAC+AGG - chr8_4:24270015-24270034 Msa1320070:intron 30.0%
!!! CTGATTTTAGAATGAAGACA+GGG - chr8_4:24270016-24270035 Msa1320070:intron 30.0%
ATGCAAGTTACATGAATAGA+CGG - chr8_4:24270283-24270302 Msa1320070:intron 30.0%
ATTCAAAGCAAGAATCAATG+TGG + chr8_4:24270464-24270483 None:intergenic 30.0%
! ATTCTCACCTATTTGAAACA+AGG - chr8_4:24270503-24270522 Msa1320070:intron 30.0%
! TTGTACAATGAGGAGTTTTA+TGG + chr8_4:24270552-24270571 None:intergenic 30.0%
GATCAGAATATTGTACAATG+AGG + chr8_4:24270562-24270581 None:intergenic 30.0%
!!! TGCTGGAAATATTTTTTCGG+AGG - chr8_4:24265899-24265918 Msa1320070:three_prime_UTR 35.0%
CAAATGTATACAACCTCCAT+TGG - chr8_4:24266124-24266143 Msa1320070:three_prime_UTR 35.0%
TCACTTATTCACTATTCGCT+AGG + chr8_4:24266175-24266194 None:intergenic 35.0%
! TGCATTTCAAGTAAGTAGAC+CGG + chr8_4:24266881-24266900 None:intergenic 35.0%
!!! TAGCTTGTTTTGTGCGATAT+TGG - chr8_4:24266945-24266964 Msa1320070:CDS 35.0%
GAAAAAATATGTCTGCGTCA+TGG - chr8_4:24267249-24267268 Msa1320070:intron 35.0%
TGCCTCCAAATGTTAATTCA+TGG - chr8_4:24267389-24267408 Msa1320070:intron 35.0%
TCTTGTGTAACATGTGTTGT+TGG + chr8_4:24267442-24267461 None:intergenic 35.0%
ACAAGAAAAACGAAAGGTGA+AGG - chr8_4:24267456-24267475 Msa1320070:CDS 35.0%
! GAACTTGCATTTGGCTTATT+TGG - chr8_4:24267647-24267666 Msa1320070:CDS 35.0%
CAAAGTCGAAACACAATGTT+TGG + chr8_4:24268286-24268305 None:intergenic 35.0%
AATGCTGTATTTACCCAGTA+CGG + chr8_4:24268481-24268500 None:intergenic 35.0%
TACTGGGTAAATACAGCATT+TGG - chr8_4:24268481-24268500 Msa1320070:intron 35.0%
ATTCTTCTGTTCGGTTTATG+AGG - chr8_4:24268532-24268551 Msa1320070:intron 35.0%
TTCTTCTGTTCGGTTTATGA+GGG - chr8_4:24268533-24268552 Msa1320070:intron 35.0%
AAAGAGAGTCAACATGCTTT+AGG - chr8_4:24268642-24268661 Msa1320070:intron 35.0%
ACGTCAAGAAATAAAGGCTA+TGG - chr8_4:24268785-24268804 Msa1320070:intron 35.0%
CGTCAAGAAATAAAGGCTAT+GGG - chr8_4:24268786-24268805 Msa1320070:intron 35.0%
GATAAGCAATGAATTCCACT+TGG + chr8_4:24268960-24268979 None:intergenic 35.0%
AAATAATGCACACGGAACAT+CGG + chr8_4:24269169-24269188 None:intergenic 35.0%
ATATTCAAACCCTACAGTCA+AGG + chr8_4:24269219-24269238 None:intergenic 35.0%
AGATGGAAAACATGCTTTGA+AGG + chr8_4:24269336-24269355 None:intergenic 35.0%
CAAAAGAAACCACATGTTCA+TGG + chr8_4:24269385-24269404 None:intergenic 35.0%
!! ATTGGAAGCACTAGAAGAAA+GGG + chr8_4:24269547-24269566 None:intergenic 35.0%
!! TATTGGAAGCACTAGAAGAA+AGG + chr8_4:24269548-24269567 None:intergenic 35.0%
!!! TGAGCATCGAGAGATATTTT+GGG + chr8_4:24269584-24269603 None:intergenic 35.0%
!!! TTGAGCATCGAGAGATATTT+TGG + chr8_4:24269585-24269604 None:intergenic 35.0%
CTGGAAGATAGAATCTAAGA+TGG + chr8_4:24269608-24269627 None:intergenic 35.0%
CGATGTCATCATCAAGAAAA+AGG + chr8_4:24269656-24269675 None:intergenic 35.0%
AGAAGGATTTGACAGGATTA+TGG - chr8_4:24269682-24269701 Msa1320070:intron 35.0%
GAAGGATTTGACAGGATTAT+GGG - chr8_4:24269683-24269702 Msa1320070:intron 35.0%
! ATTATGGGAAGTTGATCTTC+AGG - chr8_4:24269698-24269717 Msa1320070:intron 35.0%
! TTATGGGAAGTTGATCTTCA+GGG - chr8_4:24269699-24269718 Msa1320070:intron 35.0%
AACTTTGATCCAAATGCTTG+TGG - chr8_4:24269810-24269829 Msa1320070:intron 35.0%
CATGAACGTATTTGATCTCA+AGG - chr8_4:24269845-24269864 Msa1320070:intron 35.0%
TGGAAAAAGAAGGATATCAC+TGG - chr8_4:24269870-24269889 Msa1320070:intron 35.0%
TCACTGGCATATATCACAAA+TGG - chr8_4:24269886-24269905 Msa1320070:intron 35.0%
CAGGACTAATGACATTCTAT+GGG - chr8_4:24270066-24270085 Msa1320070:intron 35.0%
CACATCCACTAAATAAATCG+TGG - chr8_4:24270093-24270112 Msa1320070:intron 35.0%
ACATGCCACGATTTATTTAG+TGG + chr8_4:24270101-24270120 None:intergenic 35.0%
ATCATTGATCGATCTACAGT+TGG + chr8_4:24270143-24270162 None:intergenic 35.0%
! TAACTTGCATTTCTGCAGAT+AGG + chr8_4:24270273-24270292 None:intergenic 35.0%
CAGAAAGATGAAAGAAATGG+GGG + chr8_4:24270336-24270355 None:intergenic 35.0%
CCAGAAAGATGAAAGAAATG+GGG + chr8_4:24270337-24270356 None:intergenic 35.0%
GCCAGAAAGATGAAAGAAAT+GGG + chr8_4:24270338-24270357 None:intergenic 35.0%
TGCCAGAAAGATGAAAGAAA+TGG + chr8_4:24270339-24270358 None:intergenic 35.0%
AATGTGGAAAGGAATGATGT+TGG + chr8_4:24270448-24270467 None:intergenic 35.0%
AAGCAAGAATCAATGTGGAA+AGG + chr8_4:24270459-24270478 None:intergenic 35.0%
!! GAGGAGTTTTATGGTAAACA+AGG + chr8_4:24270543-24270562 None:intergenic 35.0%
! GAGGTCAATGTGATTAGTTC+CGG + chr8_4:24265844-24265863 None:intergenic 40.0%
! CTTTGTTGGTTGATGTTGGT+AGG - chr8_4:24265943-24265962 Msa1320070:three_prime_UTR 40.0%
! TGTTGGTAGGAAGATTTCAG+CGG - chr8_4:24265956-24265975 Msa1320070:three_prime_UTR 40.0%
!!! GGATTTTCCACTTGGTTCTA+AGG - chr8_4:24266019-24266038 Msa1320070:three_prime_UTR 40.0%
CTCCATTGGACAAAAAGTAG+CGG - chr8_4:24266138-24266157 Msa1320070:three_prime_UTR 40.0%
!!! ATCCGCTACTTTTTGTCCAA+TGG + chr8_4:24266143-24266162 None:intergenic 40.0%
CATGTGAGTACGAGACAAAA+TGG + chr8_4:24266226-24266245 None:intergenic 40.0%
TGTATCCAGCACAGATGATA+TGG - chr8_4:24267202-24267221 Msa1320070:intron 40.0%
GTATCCAGCACAGATGATAT+GGG - chr8_4:24267203-24267222 Msa1320070:intron 40.0%
TATCCAGCACAGATGATATG+GGG - chr8_4:24267204-24267223 Msa1320070:intron 40.0%
CACATTAGTCCAAACCTCAT+AGG + chr8_4:24267306-24267325 None:intergenic 40.0%
GAAAAACGAAAGGTGAAGGA+AGG - chr8_4:24267460-24267479 Msa1320070:CDS 40.0%
ACAAAATGATGCTCACCCTT+CGG + chr8_4:24267612-24267631 None:intergenic 40.0%
AGAACATCGTTGTCAATGCT+TGG + chr8_4:24268256-24268275 None:intergenic 40.0%
TGGGTAAATACAGCATTTGG+AGG - chr8_4:24268484-24268503 Msa1320070:intron 40.0%
TATGAGGGACCAGATCATTA+TGG - chr8_4:24268548-24268567 Msa1320070:intron 40.0%
ATACCCTAGCCATAATGATC+TGG + chr8_4:24268560-24268579 None:intergenic 40.0%
CTGATTCAGATCTTCATCAC+AGG - chr8_4:24268676-24268695 Msa1320070:intron 40.0%
CTTCATCACAGGTTCGAATT+AGG - chr8_4:24268687-24268706 Msa1320070:intron 40.0%
GCAATTGTATGACAGCAAGT+TGG - chr8_4:24268833-24268852 Msa1320070:intron 40.0%
AGACAGCTGATGAGAAAGTT+AGG - chr8_4:24268883-24268902 Msa1320070:intron 40.0%
AATTCCACTTGGTATGGTCT+TGG + chr8_4:24268949-24268968 None:intergenic 40.0%
GCAATGAATTCCACTTGGTA+TGG + chr8_4:24268955-24268974 None:intergenic 40.0%
GGAGGTAGTAATCTATTGTG+AGG + chr8_4:24268989-24269008 None:intergenic 40.0%
!!! ACTTCCTTTTTTCAGGAGGA+AGG + chr8_4:24269010-24269029 None:intergenic 40.0%
!!! CTAGGGAACTTCCTTTTTTC+AGG + chr8_4:24269017-24269036 None:intergenic 40.0%
CTGAAAAAAGGAAGTTCCCT+AGG - chr8_4:24269015-24269034 Msa1320070:intron 40.0%
AACAGCTGAGTTCACAACAA+CGG + chr8_4:24269108-24269127 None:intergenic 40.0%
CTCAGCTGTTACAAATGCAA+AGG - chr8_4:24269118-24269137 Msa1320070:intron 40.0%
GTTCTTGTGTTCAATGTTGC+AGG - chr8_4:24269308-24269327 Msa1320070:CDS 40.0%
!!! ATTTTGGAGCCATGAACATG+TGG - chr8_4:24269373-24269392 Msa1320070:intron 40.0%
!! ACATGGATTGTAGCTTTTCC+AGG + chr8_4:24269414-24269433 None:intergenic 40.0%
TGATGACATCGTTGTTCAGA+AGG - chr8_4:24269665-24269684 Msa1320070:intron 40.0%
GTTGTTCAGAAGGATTTGAC+AGG - chr8_4:24269675-24269694 Msa1320070:intron 40.0%
! TATGGGAAGTTGATCTTCAG+GGG - chr8_4:24269700-24269719 Msa1320070:intron 40.0%
GATCTTCAGGGGAAAGTAAA+TGG - chr8_4:24269711-24269730 Msa1320070:intron 40.0%
AATGGTGCAGTTGAAACATG+TGG - chr8_4:24269729-24269748 Msa1320070:intron 40.0%
TTGATCCAAATGCTTGTGGT+TGG - chr8_4:24269814-24269833 Msa1320070:intron 40.0%
TGATCCAAATGCTTGTGGTT+GGG - chr8_4:24269815-24269834 Msa1320070:intron 40.0%
ACGTATTTGATCTCAAGGAG+TGG - chr8_4:24269850-24269869 Msa1320070:intron 40.0%
TCTCAAGGAGTGGAAAAAGA+AGG - chr8_4:24269860-24269879 Msa1320070:intron 40.0%
TAGAATGTCATTAGTCCTGG+AGG + chr8_4:24270065-24270084 None:intergenic 40.0%
CCATAGAATGTCATTAGTCC+TGG + chr8_4:24270068-24270087 None:intergenic 40.0%
CCAGGACTAATGACATTCTA+TGG - chr8_4:24270065-24270084 Msa1320070:intron 40.0%
AATAAATCGTGGCATGTCCT+TGG - chr8_4:24270104-24270123 Msa1320070:intron 40.0%
TTGGACTATAACCCAAACCA+AGG + chr8_4:24270124-24270143 None:intergenic 40.0%
GCAGCAGTTGTACATTACAA+TGG - chr8_4:24270170-24270189 Msa1320070:intron 40.0%
ACAATGGTAATATGAAGCCG+TGG - chr8_4:24270186-24270205 Msa1320070:intron 40.0%
CGATTGCCATGACAAAGTAT+CGG - chr8_4:24270213-24270232 Msa1320070:intron 40.0%
CCCCATTTCTTTCATCTTTC+TGG - chr8_4:24270334-24270353 Msa1320070:intron 40.0%
CTAGTGTCTTGCTAGAATGA+TGG - chr8_4:24270371-24270390 Msa1320070:intron 40.0%
TTCTGATCTGATGTTCAGAC+AGG - chr8_4:24270574-24270593 Msa1320070:CDS 40.0%
! AATGTGATTAGTTCCGGCAC+TGG + chr8_4:24265838-24265857 None:intergenic 45.0%
TTCGGAGGATGTTGCTGATA+TGG - chr8_4:24265914-24265933 Msa1320070:three_prime_UTR 45.0%
!! TGATATGGCTGTGGCTTTGT+TGG - chr8_4:24265929-24265948 Msa1320070:three_prime_UTR 45.0%
!! GTGGCTTTGTTGGTTGATGT+TGG - chr8_4:24265939-24265958 Msa1320070:three_prime_UTR 45.0%
AGTGGAAAATCCCAAGATGC+AGG + chr8_4:24266011-24266030 None:intergenic 45.0%
! GCATCTTGGGATTTTCCACT+TGG - chr8_4:24266011-24266030 Msa1320070:three_prime_UTR 45.0%
TTGCAGACCTTAGAACCAAG+TGG + chr8_4:24266029-24266048 None:intergenic 45.0%
!!! CGCTACTTTTTGTCCAATGG+AGG + chr8_4:24266140-24266159 None:intergenic 45.0%
TTCAGCTGCTGCAAATAGAC+AGG - chr8_4:24266922-24266941 Msa1320070:CDS 45.0%
TGCATGCCATTCTCTCCATT+TGG + chr8_4:24267050-24267069 None:intergenic 45.0%
TGAGCTTGCCTCTTTCGAAA+AGG + chr8_4:24267092-24267111 None:intergenic 45.0%
! TTTTCGAAAGAGGCAAGCTC+AGG - chr8_4:24267091-24267110 Msa1320070:CDS 45.0%
GACTTCAAATGCTGCCTATG+AGG - chr8_4:24267289-24267308 Msa1320070:intron 45.0%
CAAATGCTGCCTATGAGGTT+TGG - chr8_4:24267294-24267313 Msa1320070:intron 45.0%
GTGAAGGAAGGAAGATCTTC+AGG - chr8_4:24267472-24267491 Msa1320070:CDS 45.0%
AAGGAAGGAAGATCTTCAGG+TGG - chr8_4:24267475-24267494 Msa1320070:CDS 45.0%
AAGACGGAAGACTATCTTGG+AGG + chr8_4:24267922-24267941 None:intergenic 45.0%
GAGAAGACGGAAGACTATCT+TGG + chr8_4:24267925-24267944 None:intergenic 45.0%
CGTTGTCAATGCTTGGCAAT+TGG + chr8_4:24268249-24268268 None:intergenic 45.0%
!! TGCTGCTGAGTTGGTAAAGA+TGG - chr8_4:24268392-24268411 Msa1320070:intron 45.0%
GGACCAGATCATTATGGCTA+GGG - chr8_4:24268554-24268573 Msa1320070:intron 45.0%
TATGGGCCAAGTTCTGTCAA+AGG - chr8_4:24268803-24268822 Msa1320070:intron 45.0%
!! TATGACAGCAAGTTGGTCAC+TGG - chr8_4:24268840-24268859 Msa1320070:intron 45.0%
AAGCACATTCCTCAGTCAGT+TGG - chr8_4:24268920-24268939 Msa1320070:intron 45.0%
ACCTCCTTCCTCCTGAAAAA+AGG - chr8_4:24269003-24269022 Msa1320070:intron 45.0%
!!! TCCTTTTTTCAGGAGGAAGG+AGG + chr8_4:24269007-24269026 None:intergenic 45.0%
!!! GGGAACTTCCTTTTTTCAGG+AGG + chr8_4:24269014-24269033 None:intergenic 45.0%
TCTCCAAGTTCTCACTCCTA+GGG + chr8_4:24269034-24269053 None:intergenic 45.0%
TTCTCCAAGTTCTCACTCCT+AGG + chr8_4:24269035-24269054 None:intergenic 45.0%
! TGGTTTCTTTTGAACCCTCC+TGG - chr8_4:24269393-24269412 Msa1320070:intron 45.0%
! GGATTGTAGCTTTTCCAGGA+GGG + chr8_4:24269410-24269429 None:intergenic 45.0%
! TGGATTGTAGCTTTTCCAGG+AGG + chr8_4:24269411-24269430 None:intergenic 45.0%
ATTCAAGCTGCCGCAATACA+GGG + chr8_4:24269479-24269498 None:intergenic 45.0%
TAGAAGAAAGGGTGGTTGGA+TGG + chr8_4:24269536-24269555 None:intergenic 45.0%
TATGCCCAACCACAAGCATT+TGG + chr8_4:24269822-24269841 None:intergenic 45.0%
AATGCTTGTGGTTGGGCATA+TGG - chr8_4:24269822-24269841 Msa1320070:intron 45.0%
! AGAATGAAGACAGGGTGCTT+TGG - chr8_4:24270024-24270043 Msa1320070:intron 45.0%
GTAAGGCCGATACTTTGTCA+TGG + chr8_4:24270222-24270241 None:intergenic 45.0%
!! TGACAAAGTATCGGCCTTAC+TGG - chr8_4:24270222-24270241 Msa1320070:intron 45.0%
ATGAAAGAAATGGGGGATGC+TGG + chr8_4:24270329-24270348 None:intergenic 45.0%
TGGTCAGTCTTGAACCTAGA+AGG - chr8_4:24270391-24270410 Msa1320070:intron 45.0%
!! GCATTGACTCTGTTCCTTCT+AGG + chr8_4:24270408-24270427 None:intergenic 45.0%
GTGGAAAGGAATGATGTTGG+AGG + chr8_4:24270445-24270464 None:intergenic 45.0%
!! AAAAAATTAAATGTTAAATA+TGG + chr8_4:24267133-24267152 None:intergenic 5.0%
!!! TCTAATTAATATTATATTTT+AGG - chr8_4:24267166-24267185 Msa1320070:CDS 5.0%
ACATTGACCTCGACGTGTGT+CGG - chr8_4:24265853-24265872 Msa1320070:three_prime_UTR 50.0%
GGATGTTGCTGATATGGCTG+TGG - chr8_4:24265920-24265939 Msa1320070:three_prime_UTR 50.0%
TGGTAGGAAGATTTCAGCGG+CGG - chr8_4:24265959-24265978 Msa1320070:three_prime_UTR 50.0%
GGAAAATCCCAAGATGCAGG+CGG + chr8_4:24266008-24266027 None:intergenic 50.0%
CATTCTCTCCATTTGGCAGC+TGG + chr8_4:24267043-24267062 None:intergenic 50.0%
CAGCTGCCAAATGGAGAGAA+TGG - chr8_4:24267041-24267060 Msa1320070:CDS 50.0%
TCTGCGTCATGGAGAGTTGT+TGG - chr8_4:24267260-24267279 Msa1320070:intron 50.0%
TGGACTAATGTGCTTCTGCG+TGG - chr8_4:24267314-24267333 Msa1320070:intron 50.0%
ATCTGTTGAGTCAGCCGTAC+TGG - chr8_4:24268464-24268483 Msa1320070:intron 50.0%
TCTGTTGAGTCAGCCGTACT+GGG - chr8_4:24268465-24268484 Msa1320070:intron 50.0%
GGGACCAGATCATTATGGCT+AGG - chr8_4:24268553-24268572 Msa1320070:intron 50.0%
TGTTGCAAGAGCTGCAGTCT+AGG - chr8_4:24268616-24268635 Msa1320070:intron 50.0%
GCCAAGTTCTGTCAAAGGCA+CGG - chr8_4:24268808-24268827 Msa1320070:intron 50.0%
! CCAAGTTCTGTCAAAGGCAC+GGG - chr8_4:24268809-24268828 Msa1320070:intron 50.0%
! CAAGTTCTGTCAAAGGCACG+GGG - chr8_4:24268810-24268829 Msa1320070:intron 50.0%
! AAGTTCTGTCAAAGGCACGG+GGG - chr8_4:24268811-24268830 Msa1320070:intron 50.0%
GTTCCCTAGGAGTGAGAACT+TGG - chr8_4:24269028-24269047 Msa1320070:intron 50.0%
CATCCTACTGCCCTGTATTG+CGG - chr8_4:24269466-24269485 Msa1320070:intron 50.0%
GATTCAAGCTGCCGCAATAC+AGG + chr8_4:24269480-24269499 None:intergenic 50.0%
GCACTAGAAGAAAGGGTGGT+TGG + chr8_4:24269540-24269559 None:intergenic 50.0%
!! GGAAGCACTAGAAGAAAGGG+TGG + chr8_4:24269544-24269563 None:intergenic 50.0%
ATCGTGGCATGTCCTTGGTT+TGG - chr8_4:24270109-24270128 Msa1320070:intron 50.0%
TCGTGGCATGTCCTTGGTTT+GGG - chr8_4:24270110-24270129 Msa1320070:intron 50.0%
! GTGATTAGTTCCGGCACTGG+AGG + chr8_4:24265835-24265854 None:intergenic 55.0%
GGAATTCAGGTCGCCAATGC+TGG - chr8_4:24265882-24265901 Msa1320070:three_prime_UTR 55.0%
GGAAGATTTCAGCGGCGGAT+CGG - chr8_4:24265964-24265983 Msa1320070:three_prime_UTR 55.0%
!! CGGCGGATCGGTTTTTGAGA+AGG - chr8_4:24265976-24265995 Msa1320070:three_prime_UTR 55.0%
AGGCCCCATATCATCTGTGC+TGG + chr8_4:24267210-24267229 None:intergenic 55.0%
GTGCAGAGGGTCTTACTCAC+TGG - chr8_4:24267563-24267582 Msa1320070:CDS 55.0%
CCCTTCGGACTTGTCGTGTA+GGG + chr8_4:24267597-24267616 None:intergenic 55.0%
CCCTACACGACAAGTCCGAA+GGG - chr8_4:24267594-24267613 Msa1320070:CDS 55.0%
ACCCTTCGGACTTGTCGTGT+AGG + chr8_4:24267598-24267617 None:intergenic 55.0%
! GGAAAAGCGTGCTGCTGAGT+TGG - chr8_4:24268383-24268402 Msa1320070:intron 55.0%
CCCGTGCCTTTGACAGAACT+TGG + chr8_4:24268812-24268831 None:intergenic 55.0%
TCTTGGCAGCCAACTGACTG+AGG + chr8_4:24268932-24268951 None:intergenic 55.0%
GCTGCCAAGACCATACCAAG+TGG - chr8_4:24268942-24268961 Msa1320070:CDS 55.0%
!! AGACAGGGTGCTTTGGAAGC+TGG - chr8_4:24270031-24270050 Msa1320070:intron 55.0%
!! GACAGGGTGCTTTGGAAGCT+GGG - chr8_4:24270032-24270051 Msa1320070:intron 55.0%
!! ACAGGGTGCTTTGGAAGCTG+GGG - chr8_4:24270033-24270052 Msa1320070:intron 55.0%
AAGCTGGGGACATTACCTCC+AGG - chr8_4:24270047-24270066 Msa1320070:intron 55.0%
GTCATGGCAATCGCTAGCCA+CGG + chr8_4:24270206-24270225 None:intergenic 55.0%
GCTTCAGCCGCCTGCATCTT+GGG - chr8_4:24265998-24266017 Msa1320070:three_prime_UTR 60.0%
GACTGCAGCCAGCTGCCAAA+TGG - chr8_4:24267032-24267051 Msa1320070:CDS 60.0%
GCCCTACACGACAAGTCCGA+AGG - chr8_4:24267593-24267612 Msa1320070:CDS 60.0%
CTGCCGCAATACAGGGCAGT+AGG + chr8_4:24269472-24269491 None:intergenic 60.0%
! ACCTCGACGTGTGTCGGCGT+CGG - chr8_4:24265859-24265878 Msa1320070:three_prime_UTR 65.0%
! GTGTCGGCGTCGGGGAATTC+AGG - chr8_4:24265869-24265888 Msa1320070:three_prime_UTR 65.0%
GGCTTCAGCCGCCTGCATCT+TGG - chr8_4:24265997-24266016 Msa1320070:three_prime_UTR 65.0%
! CCTCGACGTGTGTCGGCGTC+GGG - chr8_4:24265860-24265879 Msa1320070:three_prime_UTR 70.0%
! CTCGACGTGTGTCGGCGTCG+GGG - chr8_4:24265861-24265880 Msa1320070:three_prime_UTR 70.0%
CCCGACGCCGACACACGTCG+AGG + chr8_4:24265863-24265882 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 24265828 24270715 24265828 ID=Msa1320070;Name=Msa1320070
chr8_4 mRNA 24265828 24270715 24265828 ID=Msa1320070-mRNA-1;Parent=Msa1320070;Name=Msa1320070-mRNA-1;_AED=0.10;_eAED=0.10;_QI=0|0.55|0.5|0.9|0.55|0.7|10|414|670
chr8_4 exon 24265828 24266517 24265828 ID=Msa1320070-mRNA-1:exon:8349;Parent=Msa1320070-mRNA-1
chr8_4 exon 24266626 24267213 24266626 ID=Msa1320070-mRNA-1:exon:8348;Parent=Msa1320070-mRNA-1
chr8_4 exon 24267404 24267761 24267404 ID=Msa1320070-mRNA-1:exon:8347;Parent=Msa1320070-mRNA-1
chr8_4 exon 24267846 24267946 24267846 ID=Msa1320070-mRNA-1:exon:8346;Parent=Msa1320070-mRNA-1
chr8_4 exon 24267968 24268180 24267968 ID=Msa1320070-mRNA-1:exon:8345;Parent=Msa1320070-mRNA-1
chr8_4 exon 24268928 24268974 24268928 ID=Msa1320070-mRNA-1:exon:8344;Parent=Msa1320070-mRNA-1
chr8_4 exon 24269047 24269113 24269047 ID=Msa1320070-mRNA-1:exon:8343;Parent=Msa1320070-mRNA-1
chr8_4 exon 24269233 24269355 24269233 ID=Msa1320070-mRNA-1:exon:8342;Parent=Msa1320070-mRNA-1
chr8_4 exon 24269600 24269634 24269600 ID=Msa1320070-mRNA-1:exon:8341;Parent=Msa1320070-mRNA-1
chr8_4 exon 24270511 24270715 24270511 ID=Msa1320070-mRNA-1:exon:8340;Parent=Msa1320070-mRNA-1
chr8_4 CDS 24270511 24270715 24270511 ID=Msa1320070-mRNA-1:cds;Parent=Msa1320070-mRNA-1
chr8_4 CDS 24269600 24269634 24269600 ID=Msa1320070-mRNA-1:cds;Parent=Msa1320070-mRNA-1
chr8_4 CDS 24269233 24269355 24269233 ID=Msa1320070-mRNA-1:cds;Parent=Msa1320070-mRNA-1
chr8_4 CDS 24269047 24269113 24269047 ID=Msa1320070-mRNA-1:cds;Parent=Msa1320070-mRNA-1
chr8_4 CDS 24268928 24268974 24268928 ID=Msa1320070-mRNA-1:cds;Parent=Msa1320070-mRNA-1
chr8_4 CDS 24267968 24268180 24267968 ID=Msa1320070-mRNA-1:cds;Parent=Msa1320070-mRNA-1
chr8_4 CDS 24267846 24267946 24267846 ID=Msa1320070-mRNA-1:cds;Parent=Msa1320070-mRNA-1
chr8_4 CDS 24267404 24267761 24267404 ID=Msa1320070-mRNA-1:cds;Parent=Msa1320070-mRNA-1
chr8_4 CDS 24266626 24267213 24266626 ID=Msa1320070-mRNA-1:cds;Parent=Msa1320070-mRNA-1
chr8_4 CDS 24266242 24266517 24266242 ID=Msa1320070-mRNA-1:cds;Parent=Msa1320070-mRNA-1
chr8_4 three_prime_UTR 24265828 24266241 24265828 ID=Msa1320070-mRNA-1:three_prime_utr;Parent=Msa1320070-mRNA-1
Gene Sequence

>Msa1320070

ATGACCTCCAGTGCCGGAACTAATCACATTGACCTCGACGTGTGTCGGCGTCGGGGAATTCAGGTCGCCAATGCTGGAAATATTTTTTCGGAGGATGTTGCTGATATGGCTGTGGCTTTGTTGGTTGATGTTGGTAGGAAGATTTCAGCGGCGGATCGGTTTTTGAGAAGGCTTCAGCCGCCTGCATCTTGGGATTTTCCACTTGATCAAGATGATATTTCAGCTGCTGCAAATAGACAGGTTTTCGATGTTATTGTATCCAGCACAGATGATATGGGGCCTTTGAGTCTTGACAATTTTAGAAAAAATATGTCTGCGTCATGGAGAGTTGTTGGATTAGAGACTTCAAATGCTGCCTATGAGGTAAAGCAACCAACAACACATGTTACACAAGAAAAACGAAAGGTGAAGGAAGGAAGATCTTCAGGTGAGGGTCTTACTCACTGGACTGATAGCCCTACACGACAAGTCCGAAGGCAATTAATAGAAAAAAGGCAGGAAAAGCGTGCTGCTGAGTTGGTAAAGATGGACAATGAAGTAATCGTAAAACTTGAAAATGAAGCTATTGAACGTTCAAGATCTGTTGAGTCAGCCGTACTGGGTAAATACAGCATTTGGAGGAAAGAAATTGAGAATGAAAATGCTGATTCTTCTGTTCGGTTTATGAGGGACCAGATCATTATGGCTAGGATGAACAACAAGCTTGAACTGTTGCAAGAGCTGCAGTCTAGGCTCAAAGAGAGTCAACATGCTTTAGGTGATGCAATTGCTGATTCAGATCTTCATCACAGTACACGTCAAGAAATAAAGGCTATGGGCCAAGTTCTGTCAAAGGCACGGGGGCAATTGTATGACAGCAAGTTGGTCACTGGAAAATTGAGAGCAATGCTACAGACAGCTGATGAGAAAGTTAGGAGCTTGAAGAAACAAAGCACATTCCTCAGTCAGTTGGCTGCCAAGACCATACCAAGTGGAATTCATTGCTTATCAATGCGCCTCACAATAGATTACTACCTCCTTCCTCCTGAAAAAAGGAAGTTCCCTAGGAGTGAGAACTTGGAGAATCCCAGTCTTTATCATTATGCACTATTTTCAGACAATGTCTTGGCTGCATCCGTTGTTGTGAACTCAGCTGTTACAAATGCAAAGGATCCTTCAAAGCATGTTTTCCATCTTGTTACTGATAAACTCAATTTTGGAGCCATGAACATGTGGTTTCTTTTGAACCCTCCTGGAAAAGCTACAATCCATGTTCAAAATGTAGATGATTTTAAGTGGTTGAACTCATCCTACTGCCCTGTATTGCGGCAGCTTGAATCTGCAAAAATGAAAGAGTATTATTTCAAGGCAGGCCATCCAACCACCCTTTCTTCTAGTGCTTCCAATATGAAATATAGAAACCCAAAATATCTCTCGATGCTCAACCATCTTAGATTCTATCTTCCAGAAGTTTATCCTAAATTAGATAAAATCCTTTTTCTTGATGATGACATCGTTGTTCAGAAGGATTTGACAGGATTATGGGAAGTTGATCTTCAGGGGAAAGTAAATGGTGCAGTTGAAACATGTGGTGAAAGCTTTCACCGATTTGACAAATATCTTAACTTTTCAAATCCACATATTGCAAGAAACTTTGATCCAAATGCTTGTGGTTGGGCATATGGCATGAACGTATTTGATCTCAAGGAGTGGAAAAAGAAGGATATCACTGGCATATATCACAAATGGCAAAATATGAATGAAGACAGGGTGCTTTGGAAGCTGGGGACATTACCTCCAGGACTAATGACATTCTATGGGCTGACACATCCACTAAATAAATCGTGGCATGTCCTTGGTTTGGGTTATAGTCCAACTGTAGATCGATCAATGATAGAGAATGCAGCAGTTGTACATTACAATGGTAATATGAAGCCGTGGCTAGCGATTGCCATGACAAAGTATCGGCCTTACTGGACTAAATATGTTAAATACAATCATTCCTATCTGCAGAAATGCAAGTTACATGAATAG
Protein sequence

>Msa1320070

MTSSAGTNHIDLDVCRRRGIQVANAGNIFSEDVADMAVALLVDVGRKISAADRFLRRLQPPASWDFPLDQDDISAAANRQVFDVIVSSTDDMGPLSLDNFRKNMSASWRVVGLETSNAAYEVKQPTTHVTQEKRKVKEGRSSGEGLTHWTDSPTRQVRRQLIEKRQEKRAAELVKMDNEVIVKLENEAIERSRSVESAVLGKYSIWRKEIENENADSSVRFMRDQIIMARMNNKLELLQELQSRLKESQHALGDAIADSDLHHSTRQEIKAMGQVLSKARGQLYDSKLVTGKLRAMLQTADEKVRSLKKQSTFLSQLAAKTIPSGIHCLSMRLTIDYYLLPPEKRKFPRSENLENPSLYHYALFSDNVLAASVVVNSAVTNAKDPSKHVFHLVTDKLNFGAMNMWFLLNPPGKATIHVQNVDDFKWLNSSYCPVLRQLESAKMKEYYFKAGHPTTLSSSASNMKYRNPKYLSMLNHLRFYLPEVYPKLDKILFLDDDIVVQKDLTGLWEVDLQGKVNGAVETCGESFHRFDKYLNFSNPHIARNFDPNACGWAYGMNVFDLKEWKKKDITGIYHKWQNMNEDRVLWKLGTLPPGLMTFYGLTHPLNKSWHVLGLGYSPTVDRSMIENAAVVHYNGNMKPWLAIAMTKYRPYWTKYVKYNHSYLQKCKLHE*