Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68849.t1 | XP_003610460.1 | 96.3 | 375 | 8 | 1 | 1 | 375 | 1 | 369 | 1.30E-190 | 675.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68849.t1 | Q5E930 | 55.5 | 326 | 134 | 4 | 27 | 344 | 12 | 334 | 3.2e-89 | 330.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68849.t1 | G7JJA0 | 96.3 | 375 | 8 | 1 | 1 | 375 | 1 | 369 | 9.4e-191 | 675.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene68849 | -0.860888 | 1.51E-63 | -1.69E-46 |
| MS.gene049217 | MS.gene68849 | -0.819553 | 1.09E-52 | -1.69E-46 |
| MS.gene049222 | MS.gene68849 | -0.83437 | 3.12E-56 | -1.69E-46 |
| MS.gene049285 | MS.gene68849 | -0.836531 | 8.86E-57 | -1.69E-46 |
| MS.gene049320 | MS.gene68849 | -0.830354 | 3.08E-55 | -1.69E-46 |
| MS.gene049611 | MS.gene68849 | 0.823592 | 1.28E-53 | -1.69E-46 |
| MS.gene049633 | MS.gene68849 | -0.861877 | 7.53E-64 | -1.69E-46 |
| MS.gene049634 | MS.gene68849 | 0.845079 | 5.08E-59 | -1.69E-46 |
| MS.gene050163 | MS.gene68849 | -0.826748 | 2.29E-54 | -1.69E-46 |
| MS.gene050165 | MS.gene68849 | -0.831131 | 1.99E-55 | -1.69E-46 |
| MS.gene050170 | MS.gene68849 | -0.827917 | 1.20E-54 | -1.69E-46 |
| MS.gene050323 | MS.gene68849 | -0.842933 | 1.91E-58 | -1.69E-46 |
| MS.gene050333 | MS.gene68849 | -0.835294 | 1.82E-56 | -1.69E-46 |
| MS.gene050338 | MS.gene68849 | 0.827691 | 1.36E-54 | -1.69E-46 |
| MS.gene050381 | MS.gene68849 | 0.80959 | 1.76E-50 | -1.69E-46 |
| MS.gene050444 | MS.gene68849 | -0.879143 | 1.59E-69 | -1.69E-46 |
| MS.gene050496 | MS.gene68849 | -0.834256 | 3.33E-56 | -1.69E-46 |
| MS.gene050504 | MS.gene68849 | -0.848705 | 5.17E-60 | -1.69E-46 |
| MS.gene050518 | MS.gene68849 | -0.816152 | 6.41E-52 | -1.69E-46 |
| MS.gene050577 | MS.gene68849 | -0.835291 | 1.83E-56 | -1.69E-46 |
| MS.gene050612 | MS.gene68849 | -0.873444 | 1.46E-67 | -1.69E-46 |
| MS.gene050675 | MS.gene68849 | -0.845624 | 3.62E-59 | -1.69E-46 |
| MS.gene050699 | MS.gene68849 | -0.829398 | 5.26E-55 | -1.69E-46 |
| MS.gene05079 | MS.gene68849 | 0.834672 | 2.62E-56 | -1.69E-46 |
| MS.gene050809 | MS.gene68849 | 0.84837 | 6.40E-60 | -1.69E-46 |
| MS.gene05080 | MS.gene68849 | -0.857922 | 1.17E-62 | -1.69E-46 |
| MS.gene05081 | MS.gene68849 | -0.812153 | 4.89E-51 | -1.69E-46 |
| MS.gene050868 | MS.gene68849 | -0.857897 | 1.19E-62 | -1.69E-46 |
| MS.gene050871 | MS.gene68849 | -0.853488 | 2.31E-61 | -1.69E-46 |
| MS.gene050904 | MS.gene68849 | -0.832564 | 8.79E-56 | -1.69E-46 |
| MS.gene050986 | MS.gene68849 | -0.815218 | 1.03E-51 | -1.69E-46 |
| MS.gene051044 | MS.gene68849 | 0.805557 | 1.26E-49 | -1.69E-46 |
| MS.gene05104 | MS.gene68849 | 0.831408 | 1.70E-55 | -1.69E-46 |
| MS.gene051127 | MS.gene68849 | 0.80201 | 6.87E-49 | -1.69E-46 |
| MS.gene051138 | MS.gene68849 | 0.86091 | 1.49E-63 | -1.69E-46 |
| MS.gene051228 | MS.gene68849 | -0.804946 | 1.69E-49 | -1.69E-46 |
| MS.gene051268 | MS.gene68849 | -0.823208 | 1.57E-53 | -1.69E-46 |
| MS.gene051317 | MS.gene68849 | -0.856239 | 3.68E-62 | -1.69E-46 |
| MS.gene051617 | MS.gene68849 | -0.819478 | 1.14E-52 | -1.69E-46 |
| MS.gene051632 | MS.gene68849 | 0.819551 | 1.09E-52 | -1.69E-46 |
| MS.gene051651 | MS.gene68849 | 0.85118 | 1.05E-60 | -1.69E-46 |
| MS.gene051749 | MS.gene68849 | 0.809939 | 1.48E-50 | -1.69E-46 |
| MS.gene051810 | MS.gene68849 | -0.813813 | 2.12E-51 | -1.69E-46 |
| MS.gene051838 | MS.gene68849 | -0.830381 | 3.03E-55 | -1.69E-46 |
| MS.gene051874 | MS.gene68849 | -0.832119 | 1.13E-55 | -1.69E-46 |
| MS.gene051907 | MS.gene68849 | 0.81415 | 1.78E-51 | -1.69E-46 |
| MS.gene051949 | MS.gene68849 | -0.83426 | 3.32E-56 | -1.69E-46 |
| MS.gene051973 | MS.gene68849 | 0.804329 | 2.28E-49 | -1.69E-46 |
| MS.gene052129 | MS.gene68849 | 0.884505 | 1.81E-71 | -1.69E-46 |
| MS.gene052164 | MS.gene68849 | -0.824506 | 7.78E-54 | -1.69E-46 |
| MS.gene052379 | MS.gene68849 | -0.803833 | 2.89E-49 | -1.69E-46 |
| MS.gene05240 | MS.gene68849 | 0.802747 | 4.85E-49 | -1.69E-46 |
| MS.gene052606 | MS.gene68849 | 0.805717 | 1.17E-49 | -1.69E-46 |
| MS.gene052615 | MS.gene68849 | -0.811365 | 7.26E-51 | -1.69E-46 |
| MS.gene052802 | MS.gene68849 | 0.804596 | 2.00E-49 | -1.69E-46 |
| MS.gene052805 | MS.gene68849 | 0.815321 | 9.81E-52 | -1.69E-46 |
| MS.gene052870 | MS.gene68849 | -0.843284 | 1.54E-58 | -1.69E-46 |
| MS.gene052872 | MS.gene68849 | -0.861647 | 8.86E-64 | -1.69E-46 |
| MS.gene052916 | MS.gene68849 | -0.801172 | 1.02E-48 | -1.69E-46 |
| MS.gene052966 | MS.gene68849 | -0.835287 | 1.83E-56 | -1.69E-46 |
| MS.gene053203 | MS.gene68849 | -0.830837 | 2.35E-55 | -1.69E-46 |
| MS.gene053284 | MS.gene68849 | -0.812037 | 5.19E-51 | -1.69E-46 |
| MS.gene053285 | MS.gene68849 | -0.804683 | 1.92E-49 | -1.69E-46 |
| MS.gene053420 | MS.gene68849 | 0.817134 | 3.86E-52 | -1.69E-46 |
| MS.gene053479 | MS.gene68849 | -0.842337 | 2.75E-58 | -1.69E-46 |
| MS.gene053488 | MS.gene68849 | 0.81185 | 5.70E-51 | -1.69E-46 |
| MS.gene053561 | MS.gene68849 | 0.801231 | 9.93E-49 | -1.69E-46 |
| MS.gene053632 | MS.gene68849 | -0.825407 | 4.77E-54 | -1.69E-46 |
| MS.gene053776 | MS.gene68849 | 0.809913 | 1.50E-50 | -1.69E-46 |
| MS.gene053824 | MS.gene68849 | -0.842108 | 3.16E-58 | -1.69E-46 |
| MS.gene053916 | MS.gene68849 | -0.820704 | 5.96E-53 | -1.69E-46 |
| MS.gene054290 | MS.gene68849 | 0.806699 | 7.25E-50 | -1.69E-46 |
| MS.gene054344 | MS.gene68849 | -0.803449 | 3.47E-49 | -1.69E-46 |
| MS.gene054595 | MS.gene68849 | -0.811577 | 6.53E-51 | -1.69E-46 |
| MS.gene054600 | MS.gene68849 | -0.804961 | 1.68E-49 | -1.69E-46 |
| MS.gene054642 | MS.gene68849 | -0.824048 | 9.97E-54 | -1.69E-46 |
| MS.gene054706 | MS.gene68849 | 0.833287 | 5.82E-56 | -1.69E-46 |
| MS.gene05470 | MS.gene68849 | -0.836841 | 7.39E-57 | -1.69E-46 |
| MS.gene054884 | MS.gene68849 | 0.816261 | 6.06E-52 | -1.69E-46 |
| MS.gene05494 | MS.gene68849 | -0.82776 | 1.31E-54 | -1.69E-46 |
| MS.gene054954 | MS.gene68849 | -0.830744 | 2.47E-55 | -1.69E-46 |
| MS.gene055158 | MS.gene68849 | 0.818113 | 2.32E-52 | -1.69E-46 |
| MS.gene055206 | MS.gene68849 | -0.83521 | 1.92E-56 | -1.69E-46 |
| MS.gene055362 | MS.gene68849 | -0.821143 | 4.72E-53 | -1.69E-46 |
| MS.gene055502 | MS.gene68849 | -0.857285 | 1.81E-62 | -1.69E-46 |
| MS.gene055577 | MS.gene68849 | -0.859607 | 3.68E-63 | -1.69E-46 |
| MS.gene055748 | MS.gene68849 | -0.880211 | 6.62E-70 | -1.69E-46 |
| MS.gene055750 | MS.gene68849 | -0.849377 | 3.36E-60 | -1.69E-46 |
| MS.gene055751 | MS.gene68849 | -0.88255 | 9.49E-71 | -1.69E-46 |
| MS.gene055776 | MS.gene68849 | -0.807006 | 6.24E-50 | -1.69E-46 |
| MS.gene05589 | MS.gene68849 | -0.860181 | 2.47E-63 | -1.69E-46 |
| MS.gene055941 | MS.gene68849 | -0.81904 | 1.43E-52 | -1.69E-46 |
| MS.gene055942 | MS.gene68849 | 0.808083 | 3.69E-50 | -1.69E-46 |
| MS.gene056224 | MS.gene68849 | 0.817422 | 3.32E-52 | -1.69E-46 |
| MS.gene056283 | MS.gene68849 | -0.822362 | 2.47E-53 | -1.69E-46 |
| MS.gene056387 | MS.gene68849 | -0.828911 | 6.91E-55 | -1.69E-46 |
| MS.gene056504 | MS.gene68849 | -0.846617 | 1.94E-59 | -1.69E-46 |
| MS.gene056510 | MS.gene68849 | -0.844343 | 8.02E-59 | -1.69E-46 |
| MS.gene056657 | MS.gene68849 | -0.822989 | 1.76E-53 | -1.69E-46 |
| MS.gene056678 | MS.gene68849 | 0.834813 | 2.41E-56 | -1.69E-46 |
| MS.gene056688 | MS.gene68849 | 0.817904 | 2.59E-52 | -1.69E-46 |
| MS.gene056692 | MS.gene68849 | -0.824799 | 6.64E-54 | -1.69E-46 |
| MS.gene056697 | MS.gene68849 | 0.822871 | 1.88E-53 | -1.69E-46 |
| MS.gene056856 | MS.gene68849 | 0.885124 | 1.07E-71 | -1.69E-46 |
| MS.gene056857 | MS.gene68849 | 0.813382 | 2.63E-51 | -1.69E-46 |
| MS.gene056903 | MS.gene68849 | -0.810439 | 1.15E-50 | -1.69E-46 |
| MS.gene057246 | MS.gene68849 | -0.802086 | 6.63E-49 | -1.69E-46 |
| MS.gene05753 | MS.gene68849 | -0.805103 | 1.57E-49 | -1.69E-46 |
| MS.gene057658 | MS.gene68849 | -0.808598 | 2.86E-50 | -1.69E-46 |
| MS.gene057717 | MS.gene68849 | 0.824561 | 7.55E-54 | -1.69E-46 |
| MS.gene057768 | MS.gene68849 | 0.812226 | 4.72E-51 | -1.69E-46 |
| MS.gene057831 | MS.gene68849 | -0.829199 | 5.88E-55 | -1.69E-46 |
| MS.gene057857 | MS.gene68849 | -0.807889 | 4.05E-50 | -1.69E-46 |
| MS.gene058099 | MS.gene68849 | 0.810443 | 1.15E-50 | -1.69E-46 |
| MS.gene058158 | MS.gene68849 | 0.833477 | 5.21E-56 | -1.69E-46 |
| MS.gene058171 | MS.gene68849 | 0.800398 | 1.47E-48 | -1.69E-46 |
| MS.gene058175 | MS.gene68849 | 0.80544 | 1.33E-49 | -1.69E-46 |
| MS.gene058334 | MS.gene68849 | 0.849716 | 2.70E-60 | -1.69E-46 |
| MS.gene058338 | MS.gene68849 | -0.819718 | 1.00E-52 | -1.69E-46 |
| MS.gene05837 | MS.gene68849 | -0.828301 | 9.70E-55 | -1.69E-46 |
| MS.gene058418 | MS.gene68849 | 0.81309 | 3.05E-51 | -1.69E-46 |
| MS.gene058419 | MS.gene68849 | -0.839295 | 1.73E-57 | -1.69E-46 |
| MS.gene058452 | MS.gene68849 | 0.803648 | 3.15E-49 | -1.69E-46 |
| MS.gene058544 | MS.gene68849 | -0.826411 | 2.75E-54 | -1.69E-46 |
| MS.gene058603 | MS.gene68849 | 0.855538 | 5.90E-62 | -1.69E-46 |
| MS.gene058723 | MS.gene68849 | -0.814986 | 1.16E-51 | -1.69E-46 |
| MS.gene058773 | MS.gene68849 | -0.832273 | 1.04E-55 | -1.69E-46 |
| MS.gene058838 | MS.gene68849 | -0.845371 | 4.24E-59 | -1.69E-46 |
| MS.gene059022 | MS.gene68849 | -0.840054 | 1.10E-57 | -1.69E-46 |
| MS.gene059023 | MS.gene68849 | -0.855601 | 5.66E-62 | -1.69E-46 |
| MS.gene059079 | MS.gene68849 | -0.814848 | 1.25E-51 | -1.69E-46 |
| MS.gene059102 | MS.gene68849 | 0.802736 | 4.87E-49 | -1.69E-46 |
| MS.gene05914 | MS.gene68849 | -0.829194 | 5.90E-55 | -1.69E-46 |
| MS.gene05926 | MS.gene68849 | 0.833126 | 6.38E-56 | -1.69E-46 |
| MS.gene059304 | MS.gene68849 | 0.803239 | 3.83E-49 | -1.69E-46 |
| MS.gene059526 | MS.gene68849 | 0.812255 | 4.65E-51 | -1.69E-46 |
| MS.gene05960 | MS.gene68849 | 0.815417 | 9.35E-52 | -1.69E-46 |
| MS.gene059677 | MS.gene68849 | -0.838161 | 3.39E-57 | -1.69E-46 |
| MS.gene059699 | MS.gene68849 | 0.851611 | 7.93E-61 | -1.69E-46 |
| MS.gene059701 | MS.gene68849 | 0.822401 | 2.42E-53 | -1.69E-46 |
| MS.gene059703 | MS.gene68849 | 0.854151 | 1.49E-61 | -1.69E-46 |
| MS.gene059718 | MS.gene68849 | -0.831313 | 1.79E-55 | -1.69E-46 |
| MS.gene059756 | MS.gene68849 | -0.80471 | 1.90E-49 | -1.69E-46 |
| MS.gene059865 | MS.gene68849 | -0.844146 | 9.06E-59 | -1.69E-46 |
| MS.gene059955 | MS.gene68849 | 0.805293 | 1.43E-49 | -1.69E-46 |
| MS.gene06000 | MS.gene68849 | -0.829221 | 5.81E-55 | -1.69E-46 |
| MS.gene06027 | MS.gene68849 | -0.800506 | 1.40E-48 | -1.69E-46 |
| MS.gene060405 | MS.gene68849 | 0.839485 | 1.54E-57 | -1.69E-46 |
| MS.gene060762 | MS.gene68849 | -0.863022 | 3.35E-64 | -1.69E-46 |
| MS.gene060778 | MS.gene68849 | -0.810645 | 1.04E-50 | -1.69E-46 |
| MS.gene06077 | MS.gene68849 | -0.823732 | 1.18E-53 | -1.69E-46 |
| MS.gene060823 | MS.gene68849 | 0.826244 | 3.02E-54 | -1.69E-46 |
| MS.gene060982 | MS.gene68849 | -0.855198 | 7.41E-62 | -1.69E-46 |
| MS.gene061044 | MS.gene68849 | 0.845971 | 2.91E-59 | -1.69E-46 |
| MS.gene061150 | MS.gene68849 | -0.839392 | 1.63E-57 | -1.69E-46 |
| MS.gene061231 | MS.gene68849 | -0.809656 | 1.70E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68849.t1 | MTR_4g132400 | 96.267 | 375 | 8 | 1 | 1 | 375 | 1 | 369 | 0.0 | 729 |
| MS.gene68849.t1 | MTR_4g049640 | 52.055 | 365 | 174 | 1 | 1 | 365 | 1 | 364 | 4.54e-112 | 332 |
| MS.gene68849.t1 | MTR_3g112530 | 53.030 | 330 | 153 | 2 | 45 | 373 | 43 | 371 | 2.21e-104 | 312 |
| MS.gene68849.t1 | MTR_4g101880 | 37.846 | 325 | 192 | 5 | 39 | 362 | 177 | 492 | 7.59e-65 | 216 |
| MS.gene68849.t1 | MTR_6g034030 | 35.077 | 325 | 201 | 5 | 38 | 362 | 227 | 541 | 1.06e-63 | 214 |
| MS.gene68849.t1 | MTR_0549s0020 | 34.615 | 338 | 210 | 7 | 25 | 362 | 143 | 469 | 1.20e-63 | 212 |
| MS.gene68849.t1 | MTR_6g045200 | 34.769 | 325 | 202 | 6 | 38 | 362 | 218 | 532 | 3.08e-62 | 210 |
| MS.gene68849.t1 | MTR_6g034030 | 37.891 | 256 | 151 | 4 | 38 | 293 | 227 | 474 | 1.66e-52 | 182 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68849.t1 | AT1G62280 | 53.693 | 352 | 152 | 4 | 26 | 369 | 11 | 359 | 2.06e-122 | 358 |
| MS.gene68849.t1 | AT1G62262 | 51.397 | 358 | 152 | 4 | 19 | 371 | 13 | 353 | 2.59e-117 | 345 |
| MS.gene68849.t1 | AT5G24030 | 37.812 | 320 | 189 | 4 | 43 | 362 | 253 | 562 | 3.34e-67 | 224 |
| MS.gene68849.t1 | AT1G12480 | 35.398 | 339 | 209 | 4 | 25 | 362 | 166 | 495 | 6.30e-59 | 200 |
| MS.gene68849.t1 | AT4G27970 | 34.985 | 323 | 194 | 5 | 43 | 362 | 138 | 447 | 2.84e-58 | 197 |
| MS.gene68849.t1 | AT4G27970 | 34.985 | 323 | 194 | 5 | 43 | 362 | 138 | 447 | 2.84e-58 | 197 |
| MS.gene68849.t1 | AT4G27970 | 35.370 | 311 | 185 | 5 | 55 | 362 | 1 | 298 | 8.10e-58 | 192 |
| MS.gene68849.t1 | AT4G27970 | 35.370 | 311 | 185 | 5 | 55 | 362 | 1 | 298 | 8.10e-58 | 192 |
| MS.gene68849.t1 | AT5G24030 | 38.846 | 260 | 151 | 3 | 43 | 302 | 253 | 504 | 6.49e-54 | 186 |
| MS.gene68849.t1 | AT4G27970 | 37.008 | 254 | 146 | 4 | 43 | 293 | 138 | 380 | 7.31e-48 | 167 |
| MS.gene68849.t1 | AT4G27970 | 37.008 | 254 | 146 | 4 | 43 | 293 | 138 | 380 | 7.31e-48 | 167 |
Find 61 sgRNAs with CRISPR-Local
Find 74 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTCAACCTGGTTTCTGTTAT+AGG | 0.207016 | 8.4:+1611164 | MS.gene68849:CDS |
| CTTATATAATGCAGGCTTGC+AGG | 0.280775 | 8.4:+1611535 | MS.gene68849:intron |
| GCTAAGCCAAGGAATGTTAA+AGG | 0.296789 | 8.4:-1611618 | None:intergenic |
| TCAACCTGGTTTCTGTTATA+GGG | 0.297612 | 8.4:+1611165 | MS.gene68849:CDS |
| GACCTACCTGAGAAATAAAA+AGG | 0.303132 | 8.4:-1611448 | None:intergenic |
| GAATGTGATGGCTTGGTGAT+TGG | 0.310351 | 8.4:-1610675 | None:intergenic |
| GCAATTTCAATGTATTCATT+AGG | 0.313578 | 8.4:+1611236 | MS.gene68849:CDS |
| CTGTTGCTTATCTATTACTT+TGG | 0.340533 | 8.4:+1610844 | MS.gene68849:CDS |
| GATGAATTTGCACATCATAT+TGG | 0.345252 | 8.4:+1610945 | MS.gene68849:CDS |
| TCATTCCCTTTAACATTCCT+TGG | 0.356219 | 8.4:+1611612 | MS.gene68849:CDS |
| CAAGCTAGACTTGCCATACT+TGG | 0.362804 | 8.4:-1611365 | None:intergenic |
| CACACAATTATGTCAATTGT+TGG | 0.378332 | 8.4:-1610603 | None:intergenic |
| ACAATGAATCACAAACTCTT+TGG | 0.388818 | 8.4:+1610799 | MS.gene68849:CDS |
| AATGTGATGGCTTGGTGATT+GGG | 0.406822 | 8.4:-1610674 | None:intergenic |
| TGCTTCAAGCATCACCGCCT+TGG | 0.430111 | 8.4:+1611009 | MS.gene68849:CDS |
| GCTCAAGTTATGACAGAAAT+CGG | 0.453671 | 8.4:+1611200 | MS.gene68849:CDS |
| ATAACAGAAACCAGGTTGAC+AGG | 0.456215 | 8.4:-1611161 | None:intergenic |
| TATATGTATTGTGTCTGTGC+TGG | 0.461043 | 8.4:+1611698 | MS.gene68849:CDS |
| GGTTTCTGTTATAGGGAACT+TGG | 0.463993 | 8.4:+1611172 | MS.gene68849:CDS |
| TTGGTGCTCAAGCTTTGCTA+TGG | 0.471950 | 8.4:+1610757 | MS.gene68849:CDS |
| TTAACTACATGTATACTCCT+TGG | 0.476184 | 8.4:+1610970 | MS.gene68849:CDS |
| CTAAGCCAAGGAATGTTAAA+GGG | 0.477768 | 8.4:-1611617 | None:intergenic |
| TGACTCTGAATGTGATGGCT+TGG | 0.485943 | 8.4:-1610682 | None:intergenic |
| GAGCATATAAAGAAAGGATA+AGG | 0.489737 | 8.4:-1610892 | None:intergenic |
| GCATGGATCTTAGTTAAGAA+TGG | 0.489812 | 8.4:-1610708 | None:intergenic |
| TTCAATGTATTCATTAGGAA+TGG | 0.492135 | 8.4:+1611241 | MS.gene68849:CDS |
| CAAGTATGGCAAGTCTAGCT+TGG | 0.498344 | 8.4:+1611366 | MS.gene68849:CDS |
| CTAGCTTGGAAATCCATCTC+AGG | 0.499285 | 8.4:+1611380 | MS.gene68849:CDS |
| GCACTTGAGCATATAAAGAA+AGG | 0.500953 | 8.4:-1610898 | None:intergenic |
| TGTTCAAGAAAACTATGAAA+AGG | 0.514872 | 8.4:+1611565 | MS.gene68849:CDS |
| AGCACTAGTAGTATTGTTCG+TGG | 0.515674 | 8.4:-1610631 | None:intergenic |
| GTGATGGCTTGGTGATTGGG+TGG | 0.520148 | 8.4:-1610671 | None:intergenic |
| AGTAGCAAACCCTGTCAACC+TGG | 0.530614 | 8.4:+1611151 | MS.gene68849:CDS |
| TTCTGTTATAGGGAACTTGG+TGG | 0.536173 | 8.4:+1611175 | MS.gene68849:CDS |
| CATGGCCTCTGCATTAATGC+TGG | 0.539503 | 8.4:+1611674 | MS.gene68849:CDS |
| GAATCACAAACTCTTTGGCA+TGG | 0.539825 | 8.4:+1610804 | MS.gene68849:CDS |
| AATGGTTCACTACTGAAAAG+AGG | 0.541292 | 8.4:+1611117 | MS.gene68849:CDS |
| TAAGCTGGTCTTAGCACTAC+AGG | 0.541582 | 8.4:-1611323 | None:intergenic |
| ACTATAGAATAGAATGGGTG+AGG | 0.549206 | 8.4:+1610569 | None:intergenic |
| GACGAGAAAAGCACCTGAGA+TGG | 0.550784 | 8.4:-1611393 | None:intergenic |
| AAGTTATGACAGAAATCGGT+TGG | 0.554540 | 8.4:+1611204 | MS.gene68849:CDS |
| TGATGGCTTGGTGATTGGGT+GGG | 0.556380 | 8.4:-1610670 | None:intergenic |
| CTTGCACTTTAATGCAGTGA+AGG | 0.559661 | 8.4:+1610923 | MS.gene68849:CDS |
| TAGCAAAGCTTGAGCACCAA+AGG | 0.566448 | 8.4:-1610754 | None:intergenic |
| TTCTTAACTAAGATCCATGC+AGG | 0.573358 | 8.4:+1610711 | MS.gene68849:CDS |
| TGAAAAGGCTAAACGTTACA+TGG | 0.577666 | 8.4:+1611580 | MS.gene68849:CDS |
| TAACAGAAACCAGGTTGACA+GGG | 0.582523 | 8.4:-1611160 | None:intergenic |
| AGTAATAGATAAGCAACAGA+AGG | 0.591520 | 8.4:-1610840 | None:intergenic |
| ATGAGAAGATATGAAATCCA+AGG | 0.593130 | 8.4:-1610987 | None:intergenic |
| CATATAAAGAAAGGATAAGG+TGG | 0.598970 | 8.4:-1610889 | None:intergenic |
| GGTTGTGACTCTGAATGTGA+TGG | 0.605496 | 8.4:-1610687 | None:intergenic |
| ATTCAGCACAAGCTAAGCCA+AGG | 0.637854 | 8.4:-1611629 | None:intergenic |
| TTGTGCTGAATATGCTCATG+AGG | 0.642748 | 8.4:+1611641 | MS.gene68849:CDS |
| TATAACCAGCATTAATGCAG+AGG | 0.645479 | 8.4:-1611679 | None:intergenic |
| CAAGTTCTCGAAACAATCCA+AGG | 0.648724 | 8.4:-1611026 | None:intergenic |
| GACATCATAATTCATTTACG+AGG | 0.664907 | 8.4:-1611783 | None:intergenic |
| TCATGAGGTGAAAAGTAGCA+TGG | 0.675843 | 8.4:+1611656 | MS.gene68849:CDS |
| CTTCTCAACTTTGAGCACAG+TGG | 0.684753 | 8.4:-1611742 | None:intergenic |
| AAAGGCTAAACGTTACATGG+TGG | 0.692493 | 8.4:+1611583 | MS.gene68849:CDS |
| AGTTCCCTATAACAGAAACC+AGG | 0.725065 | 8.4:-1611169 | None:intergenic |
| GTTCTCGAAACAATCCAAGG+CGG | 0.791324 | 8.4:-1611023 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAAGCAACTAAATATATAT+AGG | - | chr8.4:1611483-1611502 | None:intergenic | 15.0% |
| !! | TAAGCAACTAAATATATATA+GGG | - | chr8.4:1611482-1611501 | None:intergenic | 15.0% |
| !!! | AAATATATATAGGGTAATTT+TGG | - | chr8.4:1611473-1611492 | None:intergenic | 15.0% |
| ! | GCAATTTCAATGTATTCATT+AGG | + | chr8.4:1611236-1611255 | MS.gene68849:CDS | 25.0% |
| ! | TGTTCAAGAAAACTATGAAA+AGG | + | chr8.4:1611565-1611584 | MS.gene68849:CDS | 25.0% |
| ! | TTCAATGTATTCATTAGGAA+TGG | + | chr8.4:1611241-1611260 | MS.gene68849:CDS | 25.0% |
| !! | ATGTAAAACTTTTTGGACAA+TGG | + | chr8.4:1611099-1611118 | MS.gene68849:CDS | 25.0% |
| !! | TTTTTCATTTGTGTTTCCTT+TGG | + | chr8.4:1610738-1610757 | MS.gene68849:CDS | 25.0% |
| !!! | TTTGTTCCTTTTTATTTCTC+AGG | + | chr8.4:1611442-1611461 | MS.gene68849:CDS | 25.0% |
| ACAATGAATCACAAACTCTT+TGG | + | chr8.4:1610799-1610818 | MS.gene68849:CDS | 30.0% | |
| AGTAATAGATAAGCAACAGA+AGG | - | chr8.4:1610843-1610862 | None:intergenic | 30.0% | |
| ATGAGAAGATATGAAATCCA+AGG | - | chr8.4:1610990-1611009 | None:intergenic | 30.0% | |
| CACACAATTATGTCAATTGT+TGG | - | chr8.4:1610606-1610625 | None:intergenic | 30.0% | |
| CATATAAAGAAAGGATAAGG+TGG | - | chr8.4:1610892-1610911 | None:intergenic | 30.0% | |
| CCATTTCATTGTAATGATCA+AGG | + | chr8.4:1611512-1611531 | MS.gene68849:intron | 30.0% | |
| CCTTGATCATTACAATGAAA+TGG | - | chr8.4:1611515-1611534 | None:intergenic | 30.0% | |
| GAGCATATAAAGAAAGGATA+AGG | - | chr8.4:1610895-1610914 | None:intergenic | 30.0% | |
| GATGAATTTGCACATCATAT+TGG | + | chr8.4:1610945-1610964 | MS.gene68849:CDS | 30.0% | |
| GCAAAAAACAAGAAATAAGC+TGG | - | chr8.4:1611341-1611360 | None:intergenic | 30.0% | |
| TTAACTACATGTATACTCCT+TGG | + | chr8.4:1610970-1610989 | MS.gene68849:CDS | 30.0% | |
| ! | CTGCTTGATGTAAAACTTTT+TGG | + | chr8.4:1611092-1611111 | MS.gene68849:CDS | 30.0% |
| ! | CTGTTGCTTATCTATTACTT+TGG | + | chr8.4:1610844-1610863 | MS.gene68849:CDS | 30.0% |
| !! | TTCCTTTTTATTTCTCAGGT+AGG | + | chr8.4:1611446-1611465 | MS.gene68849:intron | 30.0% |
| AAATGAAAAAGTAGCCTGCA+TGG | - | chr8.4:1610728-1610747 | None:intergenic | 35.0% | |
| AAGTTATGACAGAAATCGGT+TGG | + | chr8.4:1611204-1611223 | MS.gene68849:CDS | 35.0% | |
| AATGGTTCACTACTGAAAAG+AGG | + | chr8.4:1611117-1611136 | MS.gene68849:CDS | 35.0% | |
| CTAAGCCAAGGAATGTTAAA+GGG | - | chr8.4:1611620-1611639 | None:intergenic | 35.0% | |
| GACCTACCTGAGAAATAAAA+AGG | - | chr8.4:1611451-1611470 | None:intergenic | 35.0% | |
| GATCAAGGCTTATATAATGC+AGG | + | chr8.4:1611527-1611546 | MS.gene68849:intron | 35.0% | |
| GCACTTGAGCATATAAAGAA+AGG | - | chr8.4:1610901-1610920 | None:intergenic | 35.0% | |
| GCATGGATCTTAGTTAAGAA+TGG | - | chr8.4:1610711-1610730 | None:intergenic | 35.0% | |
| GCTCAAGTTATGACAGAAAT+CGG | + | chr8.4:1611200-1611219 | MS.gene68849:CDS | 35.0% | |
| TATAACCAGCATTAATGCAG+AGG | - | chr8.4:1611682-1611701 | None:intergenic | 35.0% | |
| TATATGTATTGTGTCTGTGC+TGG | + | chr8.4:1611698-1611717 | MS.gene68849:CDS | 35.0% | |
| TCAACCTGGTTTCTGTTATA+GGG | + | chr8.4:1611165-1611184 | MS.gene68849:CDS | 35.0% | |
| TCATTCCCTTTAACATTCCT+TGG | + | chr8.4:1611612-1611631 | MS.gene68849:CDS | 35.0% | |
| TGAAAAGGCTAAACGTTACA+TGG | + | chr8.4:1611580-1611599 | MS.gene68849:CDS | 35.0% | |
| TTCTTAACTAAGATCCATGC+AGG | + | chr8.4:1610711-1610730 | MS.gene68849:CDS | 35.0% | |
| ! | ATAGTTTTCTTGAACAGTGC+TGG | - | chr8.4:1611561-1611580 | None:intergenic | 35.0% |
| AAAGGCTAAACGTTACATGG+TGG | + | chr8.4:1611583-1611602 | MS.gene68849:CDS | 40.0% | |
| AGCACTAGTAGTATTGTTCG+TGG | - | chr8.4:1610634-1610653 | None:intergenic | 40.0% | |
| AGTTCCCTATAACAGAAACC+AGG | - | chr8.4:1611172-1611191 | None:intergenic | 40.0% | |
| CAAGTTCTCGAAACAATCCA+AGG | - | chr8.4:1611029-1611048 | None:intergenic | 40.0% | |
| CTTATATAATGCAGGCTTGC+AGG | + | chr8.4:1611535-1611554 | MS.gene68849:intron | 40.0% | |
| CTTGCACTTTAATGCAGTGA+AGG | + | chr8.4:1610923-1610942 | MS.gene68849:CDS | 40.0% | |
| GAATCACAAACTCTTTGGCA+TGG | + | chr8.4:1610804-1610823 | MS.gene68849:CDS | 40.0% | |
| GCTAAGCCAAGGAATGTTAA+AGG | - | chr8.4:1611621-1611640 | None:intergenic | 40.0% | |
| GGTTTCTGTTATAGGGAACT+TGG | + | chr8.4:1611172-1611191 | MS.gene68849:CDS | 40.0% | |
| GTCAACCTGGTTTCTGTTAT+AGG | + | chr8.4:1611164-1611183 | MS.gene68849:CDS | 40.0% | |
| TCATGAGGTGAAAAGTAGCA+TGG | + | chr8.4:1611656-1611675 | MS.gene68849:CDS | 40.0% | |
| TTCTGTTATAGGGAACTTGG+TGG | + | chr8.4:1611175-1611194 | MS.gene68849:CDS | 40.0% | |
| TTGTGCTGAATATGCTCATG+AGG | + | chr8.4:1611641-1611660 | MS.gene68849:CDS | 40.0% | |
| ! | ATAACAGAAACCAGGTTGAC+AGG | - | chr8.4:1611164-1611183 | None:intergenic | 40.0% |
| ! | TAACAGAAACCAGGTTGACA+GGG | - | chr8.4:1611163-1611182 | None:intergenic | 40.0% |
| !! | AATGTGATGGCTTGGTGATT+GGG | - | chr8.4:1610677-1610696 | None:intergenic | 40.0% |
| !!! | GTTTTTTGCTGCACCAAGTA+TGG | + | chr8.4:1611352-1611371 | MS.gene68849:CDS | 40.0% |
| ATTCAGCACAAGCTAAGCCA+AGG | - | chr8.4:1611632-1611651 | None:intergenic | 45.0% | |
| CAAGCTAGACTTGCCATACT+TGG | - | chr8.4:1611368-1611387 | None:intergenic | 45.0% | |
| CAAGTATGGCAAGTCTAGCT+TGG | + | chr8.4:1611366-1611385 | MS.gene68849:CDS | 45.0% | |
| CTAGCTTGGAAATCCATCTC+AGG | + | chr8.4:1611380-1611399 | MS.gene68849:CDS | 45.0% | |
| CTTCTCAACTTTGAGCACAG+TGG | - | chr8.4:1611745-1611764 | None:intergenic | 45.0% | |
| GGTTGTGACTCTGAATGTGA+TGG | - | chr8.4:1610690-1610709 | None:intergenic | 45.0% | |
| GTTCTCGAAACAATCCAAGG+CGG | - | chr8.4:1611026-1611045 | None:intergenic | 45.0% | |
| TAGCAAAGCTTGAGCACCAA+AGG | - | chr8.4:1610757-1610776 | None:intergenic | 45.0% | |
| TGACTCTGAATGTGATGGCT+TGG | - | chr8.4:1610685-1610704 | None:intergenic | 45.0% | |
| ! | TTGGTGCTCAAGCTTTGCTA+TGG | + | chr8.4:1610757-1610776 | MS.gene68849:CDS | 45.0% |
| !! | GAATGTGATGGCTTGGTGAT+TGG | - | chr8.4:1610678-1610697 | None:intergenic | 45.0% |
| !! | TAAGCTGGTCTTAGCACTAC+AGG | - | chr8.4:1611326-1611345 | None:intergenic | 45.0% |
| AGTAGCAAACCCTGTCAACC+TGG | + | chr8.4:1611151-1611170 | MS.gene68849:CDS | 50.0% | |
| CATGGCCTCTGCATTAATGC+TGG | + | chr8.4:1611674-1611693 | MS.gene68849:CDS | 50.0% | |
| !! | GACGAGAAAAGCACCTGAGA+TGG | - | chr8.4:1611396-1611415 | None:intergenic | 50.0% |
| !! | TGATGGCTTGGTGATTGGGT+GGG | - | chr8.4:1610673-1610692 | None:intergenic | 50.0% |
| TGCTTCAAGCATCACCGCCT+TGG | + | chr8.4:1611009-1611028 | MS.gene68849:CDS | 55.0% | |
| !! | GTGATGGCTTGGTGATTGGG+TGG | - | chr8.4:1610674-1610693 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 1610582 | 1611794 | 1610582 | ID=MS.gene68849 |
| chr8.4 | mRNA | 1610582 | 1611794 | 1610582 | ID=MS.gene68849.t1;Parent=MS.gene68849 |
| chr8.4 | exon | 1610582 | 1611463 | 1610582 | ID=MS.gene68849.t1.exon1;Parent=MS.gene68849.t1 |
| chr8.4 | CDS | 1610582 | 1611463 | 1610582 | ID=cds.MS.gene68849.t1;Parent=MS.gene68849.t1 |
| chr8.4 | exon | 1611549 | 1611794 | 1611549 | ID=MS.gene68849.t1.exon2;Parent=MS.gene68849.t1 |
| chr8.4 | CDS | 1611549 | 1611794 | 1611549 | ID=cds.MS.gene68849.t1;Parent=MS.gene68849.t1 |
| Gene Sequence |
| Protein sequence |
