Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40773.t1 | RHN80872.1 | 82.8 | 145 | 25 | 0 | 1 | 145 | 32 | 176 | 4.60E-59 | 237.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40773.t1 | B7FM98 | 82.8 | 145 | 25 | 0 | 1 | 145 | 1 | 145 | 3.3e-59 | 237.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049346 | MS.gene40773 | 0.856379 | 3.35E-62 | -1.69E-46 |
| MS.gene049351 | MS.gene40773 | 0.826735 | 2.30E-54 | -1.69E-46 |
| MS.gene049356 | MS.gene40773 | 0.857457 | 1.61E-62 | -1.69E-46 |
| MS.gene049369 | MS.gene40773 | 0.829362 | 5.37E-55 | -1.69E-46 |
| MS.gene049491 | MS.gene40773 | 0.818534 | 1.86E-52 | -1.69E-46 |
| MS.gene049661 | MS.gene40773 | 0.834282 | 3.28E-56 | -1.69E-46 |
| MS.gene049662 | MS.gene40773 | 0.829734 | 4.36E-55 | -1.69E-46 |
| MS.gene049681 | MS.gene40773 | 0.808353 | 3.23E-50 | -1.69E-46 |
| MS.gene049758 | MS.gene40773 | 0.853561 | 2.20E-61 | -1.69E-46 |
| MS.gene049907 | MS.gene40773 | 0.811428 | 7.03E-51 | -1.69E-46 |
| MS.gene050076 | MS.gene40773 | 0.831475 | 1.63E-55 | -1.69E-46 |
| MS.gene050099 | MS.gene40773 | 0.814119 | 1.81E-51 | -1.69E-46 |
| MS.gene050128 | MS.gene40773 | 0.825692 | 4.08E-54 | -1.69E-46 |
| MS.gene050301 | MS.gene40773 | 0.876628 | 1.20E-68 | -1.69E-46 |
| MS.gene050397 | MS.gene40773 | 0.839211 | 1.81E-57 | -1.69E-46 |
| MS.gene050406 | MS.gene40773 | 0.803925 | 2.76E-49 | -1.69E-46 |
| MS.gene050432 | MS.gene40773 | 0.883444 | 4.46E-71 | -1.69E-46 |
| MS.gene050520 | MS.gene40773 | 0.808613 | 2.84E-50 | -1.69E-46 |
| MS.gene050529 | MS.gene40773 | 0.801645 | 8.16E-49 | -1.69E-46 |
| MS.gene050670 | MS.gene40773 | 0.844733 | 6.30E-59 | -1.69E-46 |
| MS.gene050767 | MS.gene40773 | 0.805174 | 1.52E-49 | -1.69E-46 |
| MS.gene050770 | MS.gene40773 | 0.807554 | 4.78E-50 | -1.69E-46 |
| MS.gene050900 | MS.gene40773 | 0.803427 | 3.50E-49 | -1.69E-46 |
| MS.gene05101 | MS.gene40773 | 0.836414 | 9.49E-57 | -1.69E-46 |
| MS.gene05105 | MS.gene40773 | 0.859581 | 3.75E-63 | -1.69E-46 |
| MS.gene051255 | MS.gene40773 | 0.841417 | 4.81E-58 | -1.69E-46 |
| MS.gene051360 | MS.gene40773 | 0.855416 | 6.40E-62 | -1.69E-46 |
| MS.gene051387 | MS.gene40773 | 0.802617 | 5.15E-49 | -1.69E-46 |
| MS.gene051525 | MS.gene40773 | 0.81034 | 1.21E-50 | -1.69E-46 |
| MS.gene051582 | MS.gene40773 | 0.824565 | 7.54E-54 | -1.69E-46 |
| MS.gene051663 | MS.gene40773 | 0.806182 | 9.32E-50 | -1.69E-46 |
| MS.gene051692 | MS.gene40773 | 0.81732 | 3.51E-52 | -1.69E-46 |
| MS.gene051860 | MS.gene40773 | 0.808298 | 3.32E-50 | -1.69E-46 |
| MS.gene052011 | MS.gene40773 | -0.810952 | 8.92E-51 | -1.69E-46 |
| MS.gene052077 | MS.gene40773 | 0.81165 | 6.30E-51 | -1.69E-46 |
| MS.gene052078 | MS.gene40773 | 0.84188 | 3.63E-58 | -1.69E-46 |
| MS.gene052178 | MS.gene40773 | 0.80175 | 7.77E-49 | -1.69E-46 |
| MS.gene052188 | MS.gene40773 | 0.880406 | 5.63E-70 | -1.69E-46 |
| MS.gene052207 | MS.gene40773 | 0.818959 | 1.49E-52 | -1.69E-46 |
| MS.gene052253 | MS.gene40773 | 0.855144 | 7.68E-62 | -1.69E-46 |
| MS.gene052255 | MS.gene40773 | 0.803426 | 3.51E-49 | -1.69E-46 |
| MS.gene052287 | MS.gene40773 | 0.812207 | 4.76E-51 | -1.69E-46 |
| MS.gene052310 | MS.gene40773 | 0.859665 | 3.53E-63 | -1.69E-46 |
| MS.gene052322 | MS.gene40773 | 0.81741 | 3.35E-52 | -1.69E-46 |
| MS.gene052326 | MS.gene40773 | 0.811124 | 8.19E-51 | -1.69E-46 |
| MS.gene052340 | MS.gene40773 | 0.886258 | 4.00E-72 | -1.69E-46 |
| MS.gene052421 | MS.gene40773 | 0.804893 | 1.74E-49 | -1.69E-46 |
| MS.gene052481 | MS.gene40773 | 0.820687 | 6.01E-53 | -1.69E-46 |
| MS.gene052484 | MS.gene40773 | 0.817275 | 3.59E-52 | -1.69E-46 |
| MS.gene052485 | MS.gene40773 | 0.881543 | 2.20E-70 | -1.69E-46 |
| MS.gene052508 | MS.gene40773 | 0.80431 | 2.30E-49 | -1.69E-46 |
| MS.gene053073 | MS.gene40773 | 0.802204 | 6.27E-49 | -1.69E-46 |
| MS.gene05316 | MS.gene40773 | 0.811085 | 8.35E-51 | -1.69E-46 |
| MS.gene053209 | MS.gene40773 | 0.887513 | 1.34E-72 | -1.69E-46 |
| MS.gene053449 | MS.gene40773 | 0.805779 | 1.13E-49 | -1.69E-46 |
| MS.gene053557 | MS.gene40773 | 0.828615 | 8.15E-55 | -1.69E-46 |
| MS.gene053758 | MS.gene40773 | 0.805945 | 1.04E-49 | -1.69E-46 |
| MS.gene053813 | MS.gene40773 | 0.83187 | 1.31E-55 | -1.69E-46 |
| MS.gene053836 | MS.gene40773 | 0.852446 | 4.59E-61 | -1.69E-46 |
| MS.gene053928 | MS.gene40773 | 0.878754 | 2.17E-69 | -1.69E-46 |
| MS.gene054010 | MS.gene40773 | 0.824239 | 8.99E-54 | -1.69E-46 |
| MS.gene054011 | MS.gene40773 | 0.820411 | 6.96E-53 | -1.69E-46 |
| MS.gene054012 | MS.gene40773 | 0.823723 | 1.19E-53 | -1.69E-46 |
| MS.gene054013 | MS.gene40773 | 0.823228 | 1.55E-53 | -1.69E-46 |
| MS.gene054123 | MS.gene40773 | 0.838225 | 3.26E-57 | -1.69E-46 |
| MS.gene054349 | MS.gene40773 | 0.831557 | 1.56E-55 | -1.69E-46 |
| MS.gene054376 | MS.gene40773 | 0.822058 | 2.90E-53 | -1.69E-46 |
| MS.gene054473 | MS.gene40773 | 0.815864 | 7.43E-52 | -1.69E-46 |
| MS.gene054556 | MS.gene40773 | 0.8237 | 1.20E-53 | -1.69E-46 |
| MS.gene05455 | MS.gene40773 | 0.804966 | 1.68E-49 | -1.69E-46 |
| MS.gene054930 | MS.gene40773 | 0.810859 | 9.35E-51 | -1.69E-46 |
| MS.gene055116 | MS.gene40773 | 0.888999 | 3.58E-73 | -1.69E-46 |
| MS.gene055277 | MS.gene40773 | 0.87392 | 1.01E-67 | -1.69E-46 |
| MS.gene055390 | MS.gene40773 | 0.835971 | 1.23E-56 | -1.69E-46 |
| MS.gene055422 | MS.gene40773 | 0.839069 | 1.98E-57 | -1.69E-46 |
| MS.gene055428 | MS.gene40773 | 0.85305 | 3.09E-61 | -1.69E-46 |
| MS.gene05553 | MS.gene40773 | 0.827146 | 1.84E-54 | -1.69E-46 |
| MS.gene055604 | MS.gene40773 | 0.857859 | 1.22E-62 | -1.69E-46 |
| MS.gene055633 | MS.gene40773 | 0.813093 | 3.05E-51 | -1.69E-46 |
| MS.gene055666 | MS.gene40773 | 0.824696 | 7.02E-54 | -1.69E-46 |
| MS.gene055712 | MS.gene40773 | 0.802661 | 5.05E-49 | -1.69E-46 |
| MS.gene055859 | MS.gene40773 | 0.802322 | 5.93E-49 | -1.69E-46 |
| MS.gene05602 | MS.gene40773 | 0.812991 | 3.21E-51 | -1.69E-46 |
| MS.gene056191 | MS.gene40773 | 0.871759 | 5.35E-67 | -1.69E-46 |
| MS.gene056407 | MS.gene40773 | 0.868806 | 4.97E-66 | -1.69E-46 |
| MS.gene056410 | MS.gene40773 | 0.852206 | 5.38E-61 | -1.69E-46 |
| MS.gene056511 | MS.gene40773 | 0.815455 | 9.16E-52 | -1.69E-46 |
| MS.gene056609 | MS.gene40773 | 0.836368 | 9.75E-57 | -1.69E-46 |
| MS.gene056612 | MS.gene40773 | 0.820407 | 6.97E-53 | -1.69E-46 |
| MS.gene056659 | MS.gene40773 | 0.849951 | 2.32E-60 | -1.69E-46 |
| MS.gene056723 | MS.gene40773 | 0.805342 | 1.40E-49 | -1.69E-46 |
| MS.gene056796 | MS.gene40773 | 0.818913 | 1.53E-52 | -1.69E-46 |
| MS.gene056902 | MS.gene40773 | 0.832334 | 1.00E-55 | -1.69E-46 |
| MS.gene056931 | MS.gene40773 | 0.805334 | 1.40E-49 | -1.69E-46 |
| MS.gene056933 | MS.gene40773 | 0.801454 | 8.94E-49 | -1.69E-46 |
| MS.gene05702 | MS.gene40773 | 0.871103 | 8.82E-67 | -1.69E-46 |
| MS.gene057121 | MS.gene40773 | 0.814693 | 1.35E-51 | -1.69E-46 |
| MS.gene057124 | MS.gene40773 | 0.838559 | 2.68E-57 | -1.69E-46 |
| MS.gene05733 | MS.gene40773 | 0.846281 | 2.40E-59 | -1.69E-46 |
| MS.gene057396 | MS.gene40773 | 0.82316 | 1.61E-53 | -1.69E-46 |
| MS.gene057448 | MS.gene40773 | 0.871207 | 8.14E-67 | -1.69E-46 |
| MS.gene057449 | MS.gene40773 | 0.850143 | 2.05E-60 | -1.69E-46 |
| MS.gene057450 | MS.gene40773 | 0.872225 | 3.75E-67 | -1.69E-46 |
| MS.gene057469 | MS.gene40773 | 0.83164 | 1.49E-55 | -1.69E-46 |
| MS.gene057749 | MS.gene40773 | 0.878208 | 3.38E-69 | -1.69E-46 |
| MS.gene057796 | MS.gene40773 | 0.827846 | 1.25E-54 | -1.69E-46 |
| MS.gene057823 | MS.gene40773 | 0.878007 | 3.98E-69 | -1.69E-46 |
| MS.gene057825 | MS.gene40773 | 0.812721 | 3.68E-51 | -1.69E-46 |
| MS.gene057927 | MS.gene40773 | 0.809848 | 1.54E-50 | -1.69E-46 |
| MS.gene058002 | MS.gene40773 | 0.881213 | 2.89E-70 | -1.69E-46 |
| MS.gene058007 | MS.gene40773 | 0.809796 | 1.58E-50 | -1.69E-46 |
| MS.gene058019 | MS.gene40773 | 0.801919 | 7.17E-49 | -1.69E-46 |
| MS.gene058082 | MS.gene40773 | 0.828704 | 7.75E-55 | -1.69E-46 |
| MS.gene05811 | MS.gene40773 | 0.870706 | 1.19E-66 | -1.69E-46 |
| MS.gene058142 | MS.gene40773 | 0.811704 | 6.13E-51 | -1.69E-46 |
| MS.gene058233 | MS.gene40773 | 0.824479 | 7.89E-54 | -1.69E-46 |
| MS.gene058293 | MS.gene40773 | 0.861913 | 7.34E-64 | -1.69E-46 |
| MS.gene058365 | MS.gene40773 | 0.878658 | 2.35E-69 | -1.69E-46 |
| MS.gene05842 | MS.gene40773 | 0.811017 | 8.64E-51 | -1.69E-46 |
| MS.gene058660 | MS.gene40773 | 0.849771 | 2.61E-60 | -1.69E-46 |
| MS.gene058710 | MS.gene40773 | 0.820136 | 8.04E-53 | -1.69E-46 |
| MS.gene058787 | MS.gene40773 | 0.834161 | 3.52E-56 | -1.69E-46 |
| MS.gene05886 | MS.gene40773 | 0.822645 | 2.12E-53 | -1.69E-46 |
| MS.gene058928 | MS.gene40773 | 0.825989 | 3.47E-54 | -1.69E-46 |
| MS.gene058948 | MS.gene40773 | 0.824354 | 8.45E-54 | -1.69E-46 |
| MS.gene058949 | MS.gene40773 | 0.855457 | 6.23E-62 | -1.69E-46 |
| MS.gene058993 | MS.gene40773 | 0.803718 | 3.05E-49 | -1.69E-46 |
| MS.gene058995 | MS.gene40773 | 0.812871 | 3.41E-51 | -1.69E-46 |
| MS.gene059011 | MS.gene40773 | 0.804156 | 2.47E-49 | -1.69E-46 |
| MS.gene05907 | MS.gene40773 | 0.880536 | 5.06E-70 | -1.69E-46 |
| MS.gene059285 | MS.gene40773 | 0.825436 | 4.69E-54 | -1.69E-46 |
| MS.gene059294 | MS.gene40773 | 0.817395 | 3.37E-52 | -1.69E-46 |
| MS.gene059366 | MS.gene40773 | 0.837154 | 6.15E-57 | -1.69E-46 |
| MS.gene059420 | MS.gene40773 | 0.859933 | 2.94E-63 | -1.69E-46 |
| MS.gene059518 | MS.gene40773 | 0.839402 | 1.62E-57 | -1.69E-46 |
| MS.gene059597 | MS.gene40773 | 0.852043 | 5.98E-61 | -1.69E-46 |
| MS.gene059674 | MS.gene40773 | 0.824592 | 7.42E-54 | -1.69E-46 |
| MS.gene059676 | MS.gene40773 | 0.872707 | 2.59E-67 | -1.69E-46 |
| MS.gene059688 | MS.gene40773 | 0.885446 | 8.08E-72 | -1.69E-46 |
| MS.gene059692 | MS.gene40773 | 0.908036 | 2.65E-81 | -1.69E-46 |
| MS.gene059695 | MS.gene40773 | 0.904923 | 7.39E-80 | -1.69E-46 |
| MS.gene059708 | MS.gene40773 | 0.821902 | 3.15E-53 | -1.69E-46 |
| MS.gene059711 | MS.gene40773 | 0.827313 | 1.68E-54 | -1.69E-46 |
| MS.gene059807 | MS.gene40773 | 0.818576 | 1.82E-52 | -1.69E-46 |
| MS.gene05984 | MS.gene40773 | 0.845632 | 3.60E-59 | -1.69E-46 |
| MS.gene059894 | MS.gene40773 | 0.848888 | 4.60E-60 | -1.69E-46 |
| MS.gene059974 | MS.gene40773 | 0.866495 | 2.73E-65 | -1.69E-46 |
| MS.gene060005 | MS.gene40773 | 0.826946 | 2.05E-54 | -1.69E-46 |
| MS.gene06037 | MS.gene40773 | 0.884221 | 2.31E-71 | -1.69E-46 |
| MS.gene060403 | MS.gene40773 | 0.835319 | 1.80E-56 | -1.69E-46 |
| MS.gene060407 | MS.gene40773 | 0.834835 | 2.38E-56 | -1.69E-46 |
| MS.gene060469 | MS.gene40773 | 0.808863 | 2.51E-50 | -1.69E-46 |
| MS.gene060495 | MS.gene40773 | 0.803485 | 3.41E-49 | -1.69E-46 |
| MS.gene060569 | MS.gene40773 | 0.864943 | 8.45E-65 | -1.69E-46 |
| MS.gene060804 | MS.gene40773 | 0.824785 | 6.69E-54 | -1.69E-46 |
| MS.gene060837 | MS.gene40773 | 0.841112 | 5.79E-58 | -1.69E-46 |
| MS.gene060986 | MS.gene40773 | 0.825504 | 4.52E-54 | -1.69E-46 |
| MS.gene061049 | MS.gene40773 | 0.887864 | 9.81E-73 | -1.69E-46 |
| MS.gene061051 | MS.gene40773 | -0.867231 | 1.59E-65 | -1.69E-46 |
| MS.gene061154 | MS.gene40773 | 0.838906 | 2.18E-57 | -1.69E-46 |
| MS.gene061159 | MS.gene40773 | 0.802488 | 5.48E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40773.t1 | MTR_1g084200 | 82.759 | 145 | 25 | 0 | 1 | 145 | 1 | 145 | 2.49e-83 | 242 |
| MS.gene40773.t1 | MTR_7g103200 | 66.406 | 128 | 43 | 0 | 1 | 128 | 1 | 128 | 5.36e-58 | 177 |
| MS.gene40773.t1 | MTR_7g111900 | 61.268 | 142 | 55 | 0 | 1 | 142 | 1 | 142 | 9.28e-58 | 177 |
| MS.gene40773.t1 | MTR_2g019480 | 43.609 | 133 | 73 | 1 | 11 | 141 | 15 | 147 | 6.52e-34 | 116 |
| MS.gene40773.t1 | MTR_2g046150 | 50.893 | 112 | 54 | 1 | 2 | 113 | 6 | 116 | 2.86e-33 | 115 |
| MS.gene40773.t1 | MTR_4g122930 | 53.636 | 110 | 49 | 1 | 1 | 110 | 1 | 108 | 1.16e-32 | 113 |
| MS.gene40773.t1 | MTR_2g091215 | 44.643 | 112 | 62 | 0 | 9 | 120 | 4 | 115 | 1.06e-21 | 84.7 |
| MS.gene40773.t1 | MTR_2g091210 | 39.252 | 107 | 65 | 0 | 14 | 120 | 9 | 115 | 1.32e-21 | 84.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40773.t1 | AT1G09310 | 67.227 | 119 | 39 | 0 | 1 | 119 | 1 | 119 | 4.46e-56 | 173 |
| MS.gene40773.t1 | AT1G56580 | 62.602 | 123 | 46 | 0 | 1 | 123 | 1 | 123 | 6.28e-54 | 167 |
| MS.gene40773.t1 | AT1G30020 | 47.959 | 98 | 51 | 0 | 13 | 110 | 15 | 112 | 1.98e-30 | 107 |
| MS.gene40773.t1 | AT5G49600 | 47.934 | 121 | 51 | 3 | 3 | 113 | 1 | 119 | 1.96e-29 | 105 |
| MS.gene40773.t1 | AT5G46230 | 39.496 | 119 | 71 | 1 | 9 | 126 | 11 | 129 | 3.89e-29 | 103 |
| MS.gene40773.t1 | AT4G24130 | 43.925 | 107 | 60 | 0 | 9 | 115 | 13 | 119 | 7.47e-29 | 103 |
Find 36 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGCTTGTTTGTTATGTTTC+AGG | 0.117943 | 1.2:-59279798 | MS.gene40773:CDS |
| CTTTGATTATGCTGCTCTTA+TGG | 0.168737 | 1.2:-59279773 | MS.gene40773:CDS |
| ATTATAATTTCATATATTCT+TGG | 0.252583 | 1.2:+59279733 | None:intergenic |
| CTATGTCCTTCAAAGGTAAA+AGG | 0.309948 | 1.2:+59280316 | None:intergenic |
| TCCATTGTATACCTCTGATT+TGG | 0.345924 | 1.2:+59280398 | None:intergenic |
| GTTGATAGGCTTGTGACTTA+TGG | 0.355562 | 1.2:-59280216 | MS.gene40773:CDS |
| TGCTTGTTTGTTATGTTTCA+GGG | 0.360540 | 1.2:-59279797 | MS.gene40773:CDS |
| TAGAAGAATGTGGATATCAT+AGG | 0.386766 | 1.2:-59280296 | MS.gene40773:CDS |
| ACAAGTTTGAGAAAGTTGAT+AGG | 0.420157 | 1.2:-59280230 | MS.gene40773:CDS |
| GGTAAGATCAAGAAGCTAAC+TGG | 0.432806 | 1.2:-59280165 | MS.gene40773:CDS |
| TATTTCCAAGAGAAGTTCCT+TGG | 0.437882 | 1.2:+59280353 | None:intergenic |
| AGGTGAAGGAGCTCTTGGTT+TGG | 0.449978 | 1.2:-59280137 | MS.gene40773:CDS |
| AAAGTCCAAGGAACTTCTCT+TGG | 0.460248 | 1.2:-59280358 | MS.gene40773:CDS |
| GAAGTTCCTTGGACTTTGCT+TGG | 0.463735 | 1.2:+59280364 | None:intergenic |
| CAGTTGGTGGATCATCCAAC+TGG | 0.480486 | 1.2:+59280097 | None:intergenic |
| TTCAAAGGTAAAAGGCCATT+GGG | 0.484390 | 1.2:+59280324 | None:intergenic |
| TGTGAAGGTGAAGGAGCTCT+TGG | 0.487405 | 1.2:-59280142 | MS.gene40773:CDS |
| CAGTTGGATGATCCACCAAC+TGG | 0.513052 | 1.2:-59280096 | MS.gene40773:intron |
| CTTCAAAGGTAAAAGGCCAT+TGG | 0.515656 | 1.2:+59280323 | None:intergenic |
| CATTCTTCTATGTCCTTCAA+AGG | 0.524774 | 1.2:+59280309 | None:intergenic |
| TGTGGATATCATAGGGAGAG+TGG | 0.541120 | 1.2:-59280288 | MS.gene40773:CDS |
| TCCACTTCATGAAATCCAGT+TGG | 0.548501 | 1.2:-59280112 | MS.gene40773:CDS |
| AAGGTAAAAGGCCATTGGGT+AGG | 0.553037 | 1.2:+59280328 | None:intergenic |
| AGAAGAATGTGGATATCATA+GGG | 0.557026 | 1.2:-59280295 | MS.gene40773:CDS |
| TCCAACTGGATTTCATGAAG+TGG | 0.564020 | 1.2:+59280111 | None:intergenic |
| CAAGAAGCTAACTGGTGTGA+AGG | 0.582814 | 1.2:-59280157 | MS.gene40773:CDS |
| TGATTTGGCTCTGATCTCAT+CGG | 0.588078 | 1.2:+59280413 | None:intergenic |
| GCTAACTGGTGTGAAGGTGA+AGG | 0.589102 | 1.2:-59280151 | MS.gene40773:CDS |
| GCCAAATCAGAGGTATACAA+TGG | 0.617305 | 1.2:-59280399 | MS.gene40773:CDS |
| TGAAGTTACTGCCACTGTTG+AGG | 0.621958 | 1.2:-59280190 | MS.gene40773:CDS |
| TTGAAGGACATAGAAGAATG+TGG | 0.634606 | 1.2:-59280306 | MS.gene40773:CDS |
| GTTACTGCCACTGTTGAGGT+TGG | 0.637022 | 1.2:-59280186 | MS.gene40773:CDS |
| TTGGAAATAAACCTACCCAA+TGG | 0.663914 | 1.2:-59280339 | MS.gene40773:CDS |
| AATCTGCCAAGCAAAGTCCA+AGG | 0.673023 | 1.2:-59280370 | MS.gene40773:CDS |
| TGAGATCAGAGCCAAATCAG+AGG | 0.680456 | 1.2:-59280409 | MS.gene40773:CDS |
| GATCTTACCAACCTCAACAG+TGG | 0.683396 | 1.2:+59280179 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CTTTTGGATAAACTTATTTA+AGG | + | chr1.2:59280286-59280305 | None:intergenic | 20.0% |
| !! | AACATAACATAAGTAGCTTT+TGG | + | chr1.2:59280302-59280321 | None:intergenic | 25.0% |
| AACTGGTAAAATCACATTCA+GGG | - | chr1.2:59280081-59280100 | MS.gene40773:intron | 30.0% | |
| AGAAGAATGTGGATATCATA+GGG | - | chr1.2:59279865-59279884 | MS.gene40773:intron | 30.0% | |
| TAGAAGAATGTGGATATCAT+AGG | - | chr1.2:59279864-59279883 | MS.gene40773:intron | 30.0% | |
| ! | ACAAGTTTGAGAAAGTTGAT+AGG | - | chr1.2:59279930-59279949 | MS.gene40773:intron | 30.0% |
| !! | ATGCTTGTTTGTTATGTTTC+AGG | - | chr1.2:59280362-59280381 | MS.gene40773:CDS | 30.0% |
| !! | TGCTTGTTTGTTATGTTTCA+GGG | - | chr1.2:59280363-59280382 | MS.gene40773:CDS | 30.0% |
| CAACTGGTAAAATCACATTC+AGG | - | chr1.2:59280080-59280099 | MS.gene40773:intron | 35.0% | |
| CATTCTTCTATGTCCTTCAA+AGG | + | chr1.2:59279854-59279873 | None:intergenic | 35.0% | |
| CTATGTCCTTCAAAGGTAAA+AGG | + | chr1.2:59279847-59279866 | None:intergenic | 35.0% | |
| CTTTGATTATGCTGCTCTTA+TGG | - | chr1.2:59280387-59280406 | MS.gene40773:CDS | 35.0% | |
| TATTTCCAAGAGAAGTTCCT+TGG | + | chr1.2:59279810-59279829 | None:intergenic | 35.0% | |
| TCCATTGTATACCTCTGATT+TGG | + | chr1.2:59279765-59279784 | None:intergenic | 35.0% | |
| TGAGATAGAAGGAGAAGTTA+AGG | - | chr1.2:59280144-59280163 | MS.gene40773:CDS | 35.0% | |
| TTCAAAGGTAAAAGGCCATT+GGG | + | chr1.2:59279839-59279858 | None:intergenic | 35.0% | |
| TTGAAGGACATAGAAGAATG+TGG | - | chr1.2:59279854-59279873 | MS.gene40773:intron | 35.0% | |
| TTGGAAATAAACCTACCCAA+TGG | - | chr1.2:59279821-59279840 | MS.gene40773:intron | 35.0% | |
| ! | AATGTGATTTTACCAGTTGG+TGG | + | chr1.2:59280079-59280098 | None:intergenic | 35.0% |
| ! | ATAACAAACAAGCATTGCTG+TGG | + | chr1.2:59280357-59280376 | None:intergenic | 35.0% |
| ! | CTGAATGTGATTTTACCAGT+TGG | + | chr1.2:59280082-59280101 | None:intergenic | 35.0% |
| AAAGTCCAAGGAACTTCTCT+TGG | - | chr1.2:59279802-59279821 | MS.gene40773:CDS | 40.0% | |
| AGGAGAAGTTAAGGATGTGA+AGG | - | chr1.2:59280153-59280172 | MS.gene40773:CDS | 40.0% | |
| ATCACATTCAGGGCTATTAC+AGG | - | chr1.2:59280091-59280110 | MS.gene40773:intron | 40.0% | |
| CTTCAAAGGTAAAAGGCCAT+TGG | + | chr1.2:59279840-59279859 | None:intergenic | 40.0% | |
| GGTAAGATCAAGAAGCTAAC+TGG | - | chr1.2:59279995-59280014 | MS.gene40773:intron | 40.0% | |
| TCCAACTGGATTTCATGAAG+TGG | + | chr1.2:59280052-59280071 | None:intergenic | 40.0% | |
| TCCACTTCATGAAATCCAGT+TGG | - | chr1.2:59280048-59280067 | MS.gene40773:intron | 40.0% | |
| TCCTTCTATCTCAAAAGCTG+AGG | + | chr1.2:59280137-59280156 | None:intergenic | 40.0% | |
| ! | CAATGGCCTTTTACCTTTGA+AGG | - | chr1.2:59279838-59279857 | MS.gene40773:intron | 40.0% |
| ! | GCCAAATCAGAGGTATACAA+TGG | - | chr1.2:59279761-59279780 | MS.gene40773:CDS | 40.0% |
| ! | GTTGATAGGCTTGTGACTTA+TGG | - | chr1.2:59279944-59279963 | MS.gene40773:intron | 40.0% |
| !! | TGATTTGGCTCTGATCTCAT+CGG | + | chr1.2:59279750-59279769 | None:intergenic | 40.0% |
| !!! | ATAGGGAGAGTGGTTTTGTT+TGG | - | chr1.2:59279882-59279901 | MS.gene40773:intron | 40.0% |
| AAGGTAAAAGGCCATTGGGT+AGG | + | chr1.2:59279835-59279854 | None:intergenic | 45.0% | |
| AATCTGCCAAGCAAAGTCCA+AGG | - | chr1.2:59279790-59279809 | MS.gene40773:CDS | 45.0% | |
| CAAGAAGCTAACTGGTGTGA+AGG | - | chr1.2:59280003-59280022 | MS.gene40773:intron | 45.0% | |
| CTATCTCAAAAGCTGAGGCA+GGG | + | chr1.2:59280132-59280151 | None:intergenic | 45.0% | |
| GAAGTTCCTTGGACTTTGCT+TGG | + | chr1.2:59279799-59279818 | None:intergenic | 45.0% | |
| GATCTTACCAACCTCAACAG+TGG | + | chr1.2:59279984-59280003 | None:intergenic | 45.0% | |
| TCTATCTCAAAAGCTGAGGC+AGG | + | chr1.2:59280133-59280152 | None:intergenic | 45.0% | |
| TGAAGTTACTGCCACTGTTG+AGG | - | chr1.2:59279970-59279989 | MS.gene40773:intron | 45.0% | |
| TGAGATCAGAGCCAAATCAG+AGG | - | chr1.2:59279751-59279770 | MS.gene40773:CDS | 45.0% | |
| TGCTGCAGTTGAAGTCAAAG+AGG | - | chr1.2:59280225-59280244 | MS.gene40773:CDS | 45.0% | |
| TGTGGATATCATAGGGAGAG+TGG | - | chr1.2:59279872-59279891 | MS.gene40773:intron | 45.0% | |
| !!! | GCCTCAGCTTTTGAGATAGA+AGG | - | chr1.2:59280133-59280152 | MS.gene40773:CDS | 45.0% |
| AGGTGAAGGAGCTCTTGGTT+TGG | - | chr1.2:59280023-59280042 | MS.gene40773:intron | 50.0% | |
| CAGTTGGATGATCCACCAAC+TGG | - | chr1.2:59280064-59280083 | MS.gene40773:intron | 50.0% | |
| CATTGCTGTGGAGCGAGAAA+CGG | + | chr1.2:59280345-59280364 | None:intergenic | 50.0% | |
| GCTAACTGGTGTGAAGGTGA+AGG | - | chr1.2:59280009-59280028 | MS.gene40773:intron | 50.0% | |
| GTTACTGCCACTGTTGAGGT+TGG | - | chr1.2:59279974-59279993 | MS.gene40773:intron | 50.0% | |
| TGTGAAGGTGAAGGAGCTCT+TGG | - | chr1.2:59280018-59280037 | MS.gene40773:intron | 50.0% | |
| !! | CAGTTGGTGGATCATCCAAC+TGG | + | chr1.2:59280066-59280085 | None:intergenic | 50.0% |
| CTGTGGAGCGAGAAACGGAA+AGG | + | chr1.2:59280340-59280359 | None:intergenic | 55.0% | |
| ACTGCAGCAGCAGTAGCAGC+AGG | + | chr1.2:59280214-59280233 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 59279734 | 59280448 | 59279734 | ID=MS.gene40773 |
| chr1.2 | mRNA | 59279734 | 59280448 | 59279734 | ID=MS.gene40773.t1;Parent=MS.gene40773 |
| chr1.2 | exon | 59280097 | 59280448 | 59280097 | ID=MS.gene40773.t1.exon1;Parent=MS.gene40773.t1 |
| chr1.2 | CDS | 59280097 | 59280448 | 59280097 | ID=cds.MS.gene40773.t1;Parent=MS.gene40773.t1 |
| chr1.2 | exon | 59279734 | 59279822 | 59279734 | ID=MS.gene40773.t1.exon2;Parent=MS.gene40773.t1 |
| chr1.2 | CDS | 59279734 | 59279822 | 59279734 | ID=cds.MS.gene40773.t1;Parent=MS.gene40773.t1 |
| Gene Sequence |
| Protein sequence |
