AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene45532


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45532.t1 MTR_2g097680 92.398 171 7 4 1 170 18 183 5.09e-80 235
MS.gene45532.t1 MTR_1g071380 90.643 171 8 6 1 170 1 164 8.29e-77 226
MS.gene45532.t1 MTR_2g097680 89.831 118 6 4 1 117 18 130 2.81e-57 176
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45532.t1 AT2G29180 41.772 158 75 5 20 163 5 159 4.46e-24 92.8

Find 29 sgRNAs with CRISPR-Local

Find 73 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCTTATCCGTTATCACTAT+TGG 0.245133 2.1:+6418811 MS.gene45532:CDS
GCTTATCCGTTATCACTATT+GGG 0.260636 2.1:+6418812 MS.gene45532:CDS
TCTGGTTGTAGTAGTGATAA+TGG 0.300412 2.1:-6418617 None:intergenic
GAAAGGAAGGTTGCAATCTT+TGG 0.300675 2.1:-6418520 None:intergenic
GCTGTAGCAGCAACAGGATT+TGG 0.334339 2.1:-6418676 None:intergenic
TTCAAAGGAAGTCCAAACTA+TGG 0.343791 2.1:+6418653 MS.gene45532:CDS
CATTGATGCTTCGGAGCAAT+TGG 0.360695 2.1:+6418720 MS.gene45532:CDS
CTGGTTGTAGTAGTGATAAT+GGG 0.369858 2.1:-6418616 None:intergenic
AACAGGATTTGGCCATAGTT+TGG 0.372494 2.1:-6418665 None:intergenic
AAAAGAAAAGAAAAGTAGTA+AGG 0.384781 2.1:+6419801 MS.gene45532:CDS
AGGGCAGGACCTAAAGGATT+TGG 0.404020 2.1:+6419821 MS.gene45532:CDS
GAGAAAGATGAAGAAGTTGT+TGG 0.423658 2.1:-6418592 None:intergenic
TGCTACAGCCATTGATGCTT+CGG 0.431580 2.1:+6418711 MS.gene45532:CDS
TCAACAGCTGTAGCAGCAAC+AGG 0.437132 2.1:-6418682 None:intergenic
CAATTGCTCCGAAGCATCAA+TGG 0.447559 2.1:-6418719 None:intergenic
AGAAGATGATGTTGAAAGGA+AGG 0.478894 2.1:-6418533 None:intergenic
TCATCAACTACCACTGATGA+TGG 0.480753 2.1:+6418745 MS.gene45532:CDS
AAAGAAAAGAAAAGTAGTAA+GGG 0.491413 2.1:+6419802 MS.gene45532:CDS
AAGAAGAAGATGATGTTGAA+AGG 0.496918 2.1:-6418537 None:intergenic
GTAGTAAGGGCAGGACCTAA+AGG 0.510055 2.1:+6419815 MS.gene45532:CDS
CTTATCCGTTATCACTATTG+GGG 0.525510 2.1:+6418813 MS.gene45532:CDS
CATCAACTACCACTGATGAT+GGG 0.525641 2.1:+6418746 MS.gene45532:CDS
AAAAGAAAAGTAGTAAGGGC+AGG 0.536130 2.1:+6419806 MS.gene45532:CDS
ATATACCCCAATAGTGATAA+CGG 0.550650 2.1:-6418818 None:intergenic
TTTCTTGACAAAGAGAGAGA+AGG 0.571247 2.1:+6419739 MS.gene45532:CDS
ATCAACTACCACTGATGATG+GGG 0.608198 2.1:+6418747 MS.gene45532:CDS
AAGAGACACCCCATCATCAG+TGG 0.647668 2.1:-6418755 None:intergenic
GAGAAAGAAGAAGCAGCTAG+CGG 0.687353 2.1:+6419770 MS.gene45532:CDS
TGATGATGCAAATAATGCAG+TGG 0.718262 2.1:-6418488 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTCTATATTAATAAAAGAAT+TGG - chr2.1:6418878-6418897 None:intergenic 15.0%
!! TATTCAAAATGAACTAATTA+GGG - chr2.1:6419227-6419246 None:intergenic 15.0%
!!! AAATGATTATGAACTAATTT+TGG - chr2.1:6419200-6419219 None:intergenic 15.0%
!!! AATAGAATTTTACAATATTG+AGG + chr2.1:6419243-6419262 MS.gene45532:intron 15.0%
!!! CAATTCTTTTATTAATATAG+AGG + chr2.1:6418876-6418895 MS.gene45532:intron 15.0%
!!! TTTTATAAATGTAAATGACT+TGG + chr2.1:6419587-6419606 MS.gene45532:intron 15.0%
!!! TTTTTCGTTTTGTAATTTTA+TGG + chr2.1:6419447-6419466 MS.gene45532:intron 15.0%
!! AAAAGAAAAGAAAAGTAGTA+AGG + chr2.1:6419801-6419820 MS.gene45532:CDS 20.0%
!! AAAGAAAAGAAAAGTAGTAA+GGG + chr2.1:6419802-6419821 MS.gene45532:CDS 20.0%
!! CTAAATTGATTATGAAAGTT+AGG - chr2.1:6419141-6419160 None:intergenic 20.0%
!! CTATTCAAAATGAACTAATT+AGG - chr2.1:6419228-6419247 None:intergenic 20.0%
!!! GAAAATCCATAATTTTTCAA+AGG + chr2.1:6418638-6418657 MS.gene45532:CDS 20.0%
!!! TATTGACATTACATGTTTTA+GGG + chr2.1:6419070-6419089 MS.gene45532:intron 20.0%
!!! TTATTGACATTACATGTTTT+AGG + chr2.1:6419069-6419088 MS.gene45532:intron 20.0%
!!! TTGAAAAATTATGGATTTTC+TGG - chr2.1:6418638-6418657 None:intergenic 20.0%
!!! TTGCGATTTTAATACATTAA+GGG - chr2.1:6419411-6419430 None:intergenic 20.0%
!!! TTTGCGATTTTAATACATTA+AGG - chr2.1:6419412-6419431 None:intergenic 20.0%
! TTACTTAGCATTAGTATTCA+GGG + chr2.1:6419661-6419680 MS.gene45532:intron 25.0%
! TTAGTGAAAATTATTTCCCT+TGG + chr2.1:6418986-6419005 MS.gene45532:intron 25.0%
!! TTTTGATCTTTGAATATGTG+AGG - chr2.1:6419553-6419572 None:intergenic 25.0%
AAGAAGAAGATGATGTTGAA+AGG - chr2.1:6418540-6418559 None:intergenic 30.0%
AGTAAAAACGTGTGTTGAAA+TGG - chr2.1:6418567-6418586 None:intergenic 30.0%
ATATACCCCAATAGTGATAA+CGG - chr2.1:6418821-6418840 None:intergenic 30.0%
CACATATTTAGAGACCTAAA+TGG - chr2.1:6419370-6419389 None:intergenic 30.0%
CTAGTTTATACTTTGTAGTG+TGG - chr2.1:6419044-6419063 None:intergenic 30.0%
GTTACTTAGCATTAGTATTC+AGG + chr2.1:6419660-6419679 MS.gene45532:intron 30.0%
TTTAGGTCTCTAAATATGTG+AGG + chr2.1:6419370-6419389 MS.gene45532:intron 30.0%
! ATCTTTTGACCAAATCCTTT+AGG - chr2.1:6419833-6419852 None:intergenic 30.0%
! CCTCCTATCTAAAACTATTT+CGG + chr2.1:6419636-6419655 MS.gene45532:intron 30.0%
! CTTTTCTTCATTGCTTTCAT+TGG + chr2.1:6418790-6418809 MS.gene45532:CDS 30.0%
! GGACTTCCTTTGAAAAATTA+TGG - chr2.1:6418647-6418666 None:intergenic 30.0%
! TTTAAAGCAAACAGCCATTT+AGG + chr2.1:6419353-6419372 MS.gene45532:intron 30.0%
!! TTTTTGTTTGTTTGTGTTGC+AGG + chr2.1:6419694-6419713 MS.gene45532:intron 30.0%
!!! ACACCGAAATAGTTTTAGAT+AGG - chr2.1:6419642-6419661 None:intergenic 30.0%
AAAAGAAAAGTAGTAAGGGC+AGG + chr2.1:6419806-6419825 MS.gene45532:CDS 35.0%
AGAAGATGATGTTGAAAGGA+AGG - chr2.1:6418536-6418555 None:intergenic 35.0%
ATTCCATGTCATATTGTCCA+AGG - chr2.1:6419006-6419025 None:intergenic 35.0%
CTGGTTGTAGTAGTGATAAT+GGG - chr2.1:6418619-6418638 None:intergenic 35.0%
CTTATCCGTTATCACTATTG+GGG + chr2.1:6418813-6418832 MS.gene45532:CDS 35.0%
GAGAAAGATGAAGAAGTTGT+TGG - chr2.1:6418595-6418614 None:intergenic 35.0%
GCTTATCCGTTATCACTATT+GGG + chr2.1:6418812-6418831 MS.gene45532:CDS 35.0%
TCTGGTTGTAGTAGTGATAA+TGG - chr2.1:6418620-6418639 None:intergenic 35.0%
TTCAAAGGAAGTCCAAACTA+TGG + chr2.1:6418653-6418672 MS.gene45532:CDS 35.0%
TTCCATGTCATATTGTCCAA+GGG - chr2.1:6419005-6419024 None:intergenic 35.0%
TTGAGACATGCTTGCAATTT+CGG - chr2.1:6418913-6418932 None:intergenic 35.0%
TTTCTTGACAAAGAGAGAGA+AGG + chr2.1:6419739-6419758 MS.gene45532:CDS 35.0%
! TGATGATGCAAATAATGCAG+TGG - chr2.1:6418491-6418510 None:intergenic 35.0%
!!! CCGAAATAGTTTTAGATAGG+AGG - chr2.1:6419639-6419658 None:intergenic 35.0%
AACAGGATTTGGCCATAGTT+TGG - chr2.1:6418668-6418687 None:intergenic 40.0%
AATCGCAAACACATCTCTAG+TGG + chr2.1:6419423-6419442 MS.gene45532:intron 40.0%
ACACATGCAGTCAACTTACT+AGG - chr2.1:6419166-6419185 None:intergenic 40.0%
CACATGCAGTCAACTTACTA+GGG - chr2.1:6419165-6419184 None:intergenic 40.0%
CATCAACTACCACTGATGAT+GGG + chr2.1:6418746-6418765 MS.gene45532:CDS 40.0%
CTTAGCATTAGTATTCAGGG+AGG + chr2.1:6419664-6419683 MS.gene45532:intron 40.0%
GAAAGGAAGGTTGCAATCTT+TGG - chr2.1:6418523-6418542 None:intergenic 40.0%
GGCAAATGGTCACGATTTAA+AGG + chr2.1:6419303-6419322 MS.gene45532:intron 40.0%
GGCTTATCCGTTATCACTAT+TGG + chr2.1:6418811-6418830 MS.gene45532:CDS 40.0%
TCATCAACTACCACTGATGA+TGG + chr2.1:6418745-6418764 MS.gene45532:CDS 40.0%
TTCCCTTGGACAATATGACA+TGG + chr2.1:6419000-6419019 MS.gene45532:intron 40.0%
! AGTAAGTTGACTGCATGTGT+TGG + chr2.1:6419166-6419185 MS.gene45532:intron 40.0%
! ATCAACTACCACTGATGATG+GGG + chr2.1:6418747-6418766 MS.gene45532:CDS 40.0%
CATTGATGCTTCGGAGCAAT+TGG + chr2.1:6418720-6418739 MS.gene45532:CDS 45.0%
GAGAAAGAAGAAGCAGCTAG+CGG + chr2.1:6419770-6419789 MS.gene45532:CDS 45.0%
TGCTACAGCCATTGATGCTT+CGG + chr2.1:6418711-6418730 MS.gene45532:CDS 45.0%
! CAATTGCTCCGAAGCATCAA+TGG - chr2.1:6418722-6418741 None:intergenic 45.0%
! GCCAAAACGAGTGACTAGTT+TGG + chr2.1:6419282-6419301 MS.gene45532:intron 45.0%
! GCCAAACTAGTCACTCGTTT+TGG - chr2.1:6419286-6419305 None:intergenic 45.0%
!! CGAGTGACTAGTTTGGCAAA+TGG + chr2.1:6419289-6419308 MS.gene45532:intron 45.0%
AAGAGACACCCCATCATCAG+TGG - chr2.1:6418758-6418777 None:intergenic 50.0%
AGGGCAGGACCTAAAGGATT+TGG + chr2.1:6419821-6419840 MS.gene45532:CDS 50.0%
GCTGTAGCAGCAACAGGATT+TGG - chr2.1:6418679-6418698 None:intergenic 50.0%
GTAGTAAGGGCAGGACCTAA+AGG + chr2.1:6419815-6419834 MS.gene45532:CDS 50.0%
TCAACAGCTGTAGCAGCAAC+AGG - chr2.1:6418685-6418704 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 6418484 6419877 6418484 ID=MS.gene45532
chr2.1 mRNA 6418484 6419877 6418484 ID=MS.gene45532.t1;Parent=MS.gene45532
chr2.1 exon 6418484 6418834 6418484 ID=MS.gene45532.t1.exon1;Parent=MS.gene45532.t1
chr2.1 CDS 6418484 6418834 6418484 ID=cds.MS.gene45532.t1;Parent=MS.gene45532.t1
chr2.1 exon 6419716 6419877 6419716 ID=MS.gene45532.t1.exon2;Parent=MS.gene45532.t1
chr2.1 CDS 6419716 6419877 6419716 ID=cds.MS.gene45532.t1;Parent=MS.gene45532.t1
Gene Sequence

>MS.gene45532

ATGGCCACTGCATTATTTGCATCATCATCATCATCTCCAAAGATTGCAACCTTCCTTTCAACATCATCTTCTTCTTCTTTCCATTTCAACACACGTTTTTACTCACTTCCAACAACTTCTTCATCTTTCTCTCCCATTATCACTACTACAACCAGAAAATCCATAATTTTTCAAAGGAAGTCCAAACTATGGCCAAATCCTGTTGCTGCTACAGCTGTTGAAGATGCTGCTACAGCCATTGATGCTTCGGAGCAATTGGTTTCATCAACTACCACTGATGATGGGGTGTCTCTTATCGTATCAGCTCTTTTCTTCATTGCTTTCATTGGCTTATCCGTTATCACTATTGGGGTATATTCGTCATTTTCTTCTATTCTCACTTTTTTGTTTTCCAATTCTTTTATTAATATAGAGGTAAATTATCATCCGAAATTGCAAGCATGTCTCAAAATAGTGTTTGATTTTTGTGAATTGACAAACATATTTGTGAAATTGTAAAATGTTAGTGAAAATTATTTCCCTTGGACAATATGACATGGAATCTAACATCAATGTTACCACACTACAAAGTATAAACTAGTTTGTTTATTGACATTACATGTTTTAGGGACTAATTTGTCAATAATTAATTAGTTCATGTATATTTGTAAATTACCTAACTTTCATAATCAATTTAGTCCCTAGTAAGTTGACTGCATGTGTTGGATCAACGTCCAAAATTAGTTCATAATCATTTTAGTCCCTAATTAGTTCATTTTGAATAGAATTTTACAATATTGAGGATGTATATGTCAATTCGCCAAAACGAGTGACTAGTTTGGCAAATGGTCACGATTTAAAGGATCAATTTGACTATGCGCACTCATTTGTTTAAAGCAAACAGCCATTTAGGTCTCTAAATATGTGAGGTGTTGTTAGTACAGTCCCTTAATGTATTAAAATCGCAAACACATCTCTAGTGGCTTTTTCGTTTTGTAATTTTATGGATTATATTGACTAACGAATAAAAAAAAAAACATGTTGATATGCTTGTAATTCGATTCACTCAGTGACTATAGTGACAACACCTCACATATTCAAAGATCAAAATGATTGATTATCGTTTTTATAAATGTAAATGACTTGGTAAGTTAGTAGTATTTGAACTTTTGACCTCCTATCTAAAACTATTTCGGTGTTACTTAGCATTAGTATTCAGGGAGGTTCTGTATTTTTGTTTGTTTGTGTTGCAGGTTATCTATTTAGCAGTAACAGATTTCTTGACAAAGAGAGAGAAGGAAAAATTTGAGAAAGAAGAAGCAGCTAGCGGAAAGAAGAAAAAGAAAAGAAAAGTAGTAAGGGCAGGACCTAAAGGATTTGGTCAAAAGATTGTTCAAACTGAAGATGATGATTGA

Protein sequence

>MS.gene45532.t1

MATALFASSSSSPKIATFLSTSSSSSFHFNTRFYSLPTTSSSFSPIITTTTRKSIIFQRKSKLWPNPVAATAVEDAATAIDASEQLVSSTTTDDGVSLIVSALFFIAFIGLSVITIGVIYLAVTDFLTKREKEKFEKEEAASGKKKKKRKVVRAGPKGFGQKIVQTEDDD